The Experts below are selected from a list of 912 Experts worldwide ranked by ideXlab platform
Jennifer S. Brodbelt - One of the best experts on this subject based on the ideXlab platform.
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Isolation and purification of closely related Citrus limonoid glucosides by flash chromatography
Phytochemical Analysis, 2005Co-Authors: Girija Raman, Jennifer S. Brodbelt, Bhimanagouda S. PatilAbstract:Several citrus limonoid glycosides have proved to be particularly difficult to purify using conventional techniques. A reversed-phase flash chromatographic technique has been developed for the separation and isolation of the closely related limonoid glucosides, nomilin 17-β-d-glucopyranoside and nomilinic acid 17-β-d-glucopyranoside, with confirmation of their identities by electrospray ionisation mass spectrometry. Furthermore, the semipurification of the mixture of glucosides enriched with flavanone glucosides such as naringin, Narirutin and other limonoid glucosides was obtained. The closely eluting glucosides were successfully separated to achieve a good yield and purity of 93%. Copyright © 2005 John Wiley & Sons, Ltd.
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Isolation of Structurally Similar Citrus Flavonoids by Flash Chromatography
Analytical Letters, 2004Co-Authors: Girija Raman, Guddadarangavvanahally K. Jayaprakasha, Jennifer S. Brodbelt, Bhimanagouda S. PatilAbstract:A rapid and efficient method for the extraction and isolation of naringin and Narirutin from citrus molasses is described. Naringin and Narirutin are structurally similar glucosides present in citrus molasses. The common problem in purification of plant extracts is the large number of constituents present that are similar in nature. A flash chromatographic technique has been developed for the separation of the main flavonoid glucosides present in citrus molasses. This method is a rapid, inexpensive technique and provides compounds with high purity that can be used for biological activity studies. The separation and yield of Narirutin and naringin obtained by this method is reproducible.
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screening flavonoid metabolites of naringin and Narirutin in urine after human consumption of grapefruit juice by lc ms and lc ms ms
Analyst, 2004Co-Authors: Junmei Zhang, Jennifer S. BrodbeltAbstract:The main flavonoids in grapefruit juice, naringin and Narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
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Screening flavonoid metabolites of naringin and Narirutin in urine after human consumption of grapefruit juice by LC-MS and LC-MS/MS
Analyst, 2004Co-Authors: Junmei Zhang, Jennifer S. BrodbeltAbstract:The main flavonoids in grapefruit juice, naringin and Narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
Inwook Choi - One of the best experts on this subject based on the ideXlab platform.
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Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice
Food and Chemical Toxicology, 2013Co-Authors: Hoyoung Park, Sang Keun Ha, Inwook ChoiAbstract:Abstract This study aimed to demonstrate protective activities of the Narirutin fraction from peels of Citrus unshiu against ethanol-induced hepatic damage through an animal study. Citrus Narirutin fraction (CNF), contained 75% of Narirutin, was obtained by an ultra-sonicated extraction and further purification. ICR mice were divided into four groups; normaldiet control, ethanol control (6.5 g ethanol/kg), low-CNF (ethanol + 150 mg CNF/kg) and high-CNF (ethanol + 300 mg CNF/kg) groups. Consumption of alcohol for 8 weeks induced severe liver damage with increases in prognostic indicators such as aspartate transaminase, alanine transaminase in serum whereas co-administration of CNF suppressed their increases. Excessive accumulations in liver TG and TC in ethanol control group were also suppressed by co-administration of CNF. Co-administration of CNF maintained SOD activity, GSH and malondialdehyde levels close to those of the normal diet group. Chronic consumption of alcohol also stimulated abrupt increases in pro-inflammatory cytokines such as nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver otherwise co-administration of CNF effectively suppressed production of these cytokines dose-dependently. These results indicate that co-administration of CNF with alcohol can alleviate alcohol induced liver damage through preventing lipid formation, protecting antioxidant system and suppressing productions of pro-inflammatory cytokines.
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Narirutin fraction from citrus peels attenuates lps stimulated inflammatory response through inhibition of nf κb and mapks activation
Food and Chemical Toxicology, 2012Co-Authors: Sang Keun Ha, Hoyoung Park, Inwook ChoiAbstract:In this study, we examined the regulatory activity of Narirutin fraction from citrus peels on the production of inflammatory mediators managing acute or chronic inflammatory diseases in macrophages. Narirutin fraction inhibited the release, by lipopolysaccharide (LPS)-stimulated macrophages, of nitric oxide (NO) and prostaglandin E2 (PGE2) through suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. The release, by LPS stimulated macrophages, of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was also reduced by Narirutin fraction in a dose-dependent manner. Furthermore, Narirutin fraction inhibited the LPS-mediated activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs), which are signaling molecules involved in production of pro-inflammatory factors. As a result of these properties, Narirutin fraction has the potential to be used as a functional dietary supplement and effective anti-inflammatory agent.
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characteristics of alcohol fermentation in citrus hydrolysates through Narirutin addition
Korean Journal of Food Preservation, 2012Co-Authors: Nahye Sung, Yongjun Jo, Chanwoo Park, Seyoung Jang, Inwook Choi, Yongjin JeongAbstract:The characteristics of the Narirutin-added alcohol fermentation of citrus hydrolysates were investigated to produce functionally improved citrus wine. The pH of Narirutin-added citrus wine ranged from 3.38 to 3.51, and the titratable acidity was about 0.55% which show no significant differences attributable to the Narirutin quantity. With regard to the color and brown color, with the addition of more Narirutin, the a value was decreased while the b value and brown color increased. The sugar content decreased from 24 to 8.0-8.5 after alcohol fermentation, and the alcohol content appeared to be between 14.5 and 14.6% in all the sections. For the free sugar contents, the glucose and fructose contents were relatively high in every section. For the total polyphenol content, it was shown to increase when more Narirutin was added. and the 2%-Narirutin-added section showed the highest polyphenol content(46.44 mg%). The results of sensory evaluation were that the color, flavor and bitterness increased while the sweetness and overall acceptability were reduced by the additional Narirutin. Functionally improved citrus wine can be produced by using Narirutin, but more researches regarding quality improvement should be carried out to overcome the bitterness.
Junmei Zhang - One of the best experts on this subject based on the ideXlab platform.
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screening flavonoid metabolites of naringin and Narirutin in urine after human consumption of grapefruit juice by lc ms and lc ms ms
Analyst, 2004Co-Authors: Junmei Zhang, Jennifer S. BrodbeltAbstract:The main flavonoids in grapefruit juice, naringin and Narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
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Screening flavonoid metabolites of naringin and Narirutin in urine after human consumption of grapefruit juice by LC-MS and LC-MS/MS
Analyst, 2004Co-Authors: Junmei Zhang, Jennifer S. BrodbeltAbstract:The main flavonoids in grapefruit juice, naringin and Narirutin, were quantified by LC-MS with structural differentiation by LC-MS/MS. After human consumption of grapefruit juice, urine samples were collected for 24 hours and screened for flavonoid metabolites by LC-MS. The metabolite structures (glucuronides, sulfates, and glucuronide sulfates) were then confirmed via their unique fragmentation patterns by LC-MS/MS. To further verify the identity of the common aglycon (naringenin) shared by the metabolites, enzymatic hydrolysis was performed and the resulting products were analyzed. This work demonstrates that LC-MS and LC-MS/MS techniques can be used for fast metabolite screening without extensive sample preparation.
Sang Keun Ha - One of the best experts on this subject based on the ideXlab platform.
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Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice
Food and Chemical Toxicology, 2013Co-Authors: Hoyoung Park, Sang Keun Ha, Inwook ChoiAbstract:Abstract This study aimed to demonstrate protective activities of the Narirutin fraction from peels of Citrus unshiu against ethanol-induced hepatic damage through an animal study. Citrus Narirutin fraction (CNF), contained 75% of Narirutin, was obtained by an ultra-sonicated extraction and further purification. ICR mice were divided into four groups; normaldiet control, ethanol control (6.5 g ethanol/kg), low-CNF (ethanol + 150 mg CNF/kg) and high-CNF (ethanol + 300 mg CNF/kg) groups. Consumption of alcohol for 8 weeks induced severe liver damage with increases in prognostic indicators such as aspartate transaminase, alanine transaminase in serum whereas co-administration of CNF suppressed their increases. Excessive accumulations in liver TG and TC in ethanol control group were also suppressed by co-administration of CNF. Co-administration of CNF maintained SOD activity, GSH and malondialdehyde levels close to those of the normal diet group. Chronic consumption of alcohol also stimulated abrupt increases in pro-inflammatory cytokines such as nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver otherwise co-administration of CNF effectively suppressed production of these cytokines dose-dependently. These results indicate that co-administration of CNF with alcohol can alleviate alcohol induced liver damage through preventing lipid formation, protecting antioxidant system and suppressing productions of pro-inflammatory cytokines.
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Narirutin fraction from citrus peels attenuates lps stimulated inflammatory response through inhibition of nf κb and mapks activation
Food and Chemical Toxicology, 2012Co-Authors: Sang Keun Ha, Hoyoung Park, Inwook ChoiAbstract:In this study, we examined the regulatory activity of Narirutin fraction from citrus peels on the production of inflammatory mediators managing acute or chronic inflammatory diseases in macrophages. Narirutin fraction inhibited the release, by lipopolysaccharide (LPS)-stimulated macrophages, of nitric oxide (NO) and prostaglandin E2 (PGE2) through suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. The release, by LPS stimulated macrophages, of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was also reduced by Narirutin fraction in a dose-dependent manner. Furthermore, Narirutin fraction inhibited the LPS-mediated activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs), which are signaling molecules involved in production of pro-inflammatory factors. As a result of these properties, Narirutin fraction has the potential to be used as a functional dietary supplement and effective anti-inflammatory agent.
Hoyoung Park - One of the best experts on this subject based on the ideXlab platform.
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Narirutin fraction from citrus peels attenuates alcoholic liver disease in mice
Food and Chemical Toxicology, 2013Co-Authors: Hoyoung Park, Sang Keun Ha, Inwook ChoiAbstract:Abstract This study aimed to demonstrate protective activities of the Narirutin fraction from peels of Citrus unshiu against ethanol-induced hepatic damage through an animal study. Citrus Narirutin fraction (CNF), contained 75% of Narirutin, was obtained by an ultra-sonicated extraction and further purification. ICR mice were divided into four groups; normaldiet control, ethanol control (6.5 g ethanol/kg), low-CNF (ethanol + 150 mg CNF/kg) and high-CNF (ethanol + 300 mg CNF/kg) groups. Consumption of alcohol for 8 weeks induced severe liver damage with increases in prognostic indicators such as aspartate transaminase, alanine transaminase in serum whereas co-administration of CNF suppressed their increases. Excessive accumulations in liver TG and TC in ethanol control group were also suppressed by co-administration of CNF. Co-administration of CNF maintained SOD activity, GSH and malondialdehyde levels close to those of the normal diet group. Chronic consumption of alcohol also stimulated abrupt increases in pro-inflammatory cytokines such as nuclear factor (NF)-κB, tumor necrosis factor (TNF)-α and interleukin (IL)-1β in liver otherwise co-administration of CNF effectively suppressed production of these cytokines dose-dependently. These results indicate that co-administration of CNF with alcohol can alleviate alcohol induced liver damage through preventing lipid formation, protecting antioxidant system and suppressing productions of pro-inflammatory cytokines.
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Narirutin fraction from citrus peels attenuates lps stimulated inflammatory response through inhibition of nf κb and mapks activation
Food and Chemical Toxicology, 2012Co-Authors: Sang Keun Ha, Hoyoung Park, Inwook ChoiAbstract:In this study, we examined the regulatory activity of Narirutin fraction from citrus peels on the production of inflammatory mediators managing acute or chronic inflammatory diseases in macrophages. Narirutin fraction inhibited the release, by lipopolysaccharide (LPS)-stimulated macrophages, of nitric oxide (NO) and prostaglandin E2 (PGE2) through suppressing the expression of inducible NO synthase (iNOS) and cyclooxygenase-2 (COX-2), respectively. The release, by LPS stimulated macrophages, of interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) was also reduced by Narirutin fraction in a dose-dependent manner. Furthermore, Narirutin fraction inhibited the LPS-mediated activation of nuclear factor-κB (NF-κB) and mitogen-activated protein kinases (MAPKs), which are signaling molecules involved in production of pro-inflammatory factors. As a result of these properties, Narirutin fraction has the potential to be used as a functional dietary supplement and effective anti-inflammatory agent.
