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Wei Zhou - One of the best experts on this subject based on the ideXlab platform.
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Study on the main components interaction from Flos Lonicerae and Fructus Forsythiae and their dissolution in vitro and intestinal absorption in rats.
PloS one, 2014Co-Authors: Wei Zhou, Jinjun Shan, Xiaobin Tan, Shouchuan Wang, Ailing Yin, Baochang CaiAbstract:The Flos Lonicerae-Fructus Forsythiae herb couple is the basic components of Chinese herbal preparations (Shuang-Huang-Lian tablet, Yin-Qiao-Jie-Du tablet and Fufang Qin-Lan oral liquid), and its pharmacological effects were significantly higher than that in Flos Lonicerae or Fructus Forsythiae, but the reasons remained unknown. In the present study, pattern recognition analysis (hierarchical cluster analysis (HCA) and principal component analysis (PCA)) combined with UHPLC-ESI/LTQ-Orbitrap MS system were performed to study the chemical constitution difference between co-decoction and mixed decoction in the term of chemistry. Besides, the pharmacokinetics in vivo and intestinal absorption in vitro combined with pattern recognition analysis were used to reveal the discrepancy between herb couple and single herbs in the view of biology. The observation from the chemical view in vitro showed that there was significant difference in quantity between co-decoction and mixed decoction by HCA, and the exposure level of isoforsythoside and 3, 5-dicaffeoylquinic Acid in co-decoction, higher than that in mixed decoction, directly resulted in the discrepancy between co-decoction and mixed decoction using both PCA and HCA. The observation from the pharmacokinetics displayed that the exposure level in vivo of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A, higher than that in single herbs, was the main factor contributing to the difference by both PCA and HCA, interestingly consistent with the results obtained from Caco-2 cells in vitro, which indicated that it was because of intestinal absorption improvement of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A that resulted in a better efficacy of herb couple than that of single herbs from the perspective of biology. The results above illustrated that caffeic Acid derivatives in Flos Lonicerae-Fructus Forsythiae herb couple could be considered as chemical markers for quality control of its preparations.
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simultaneous determination of caffeic Acid derivatives by uplc ms ms in rat plasma and its application in pharmacokinetic study after oral administration of flos lonicerae fructus forsythiae herb combination
Journal of Chromatography B, 2014Co-Authors: Wei Zhou, Wenzheng Ju, Jinjun Shan, Minxin Meng, Shouquan Wang, Liuqing DiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of eight caffeic Acid derivatives (forsythoside A, isoforsythoside, forsythoside B, Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae–Fructus Forsythiae herb combination in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine the eight caffeic Acid derivatives simultaneously in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with n-butyl alcohol/ethyl acetate (7:3, v/v). The separation was performed on an Acquity UPLC HSS T3 C18 column (100 mm × 2.1 mm, 1.8 μm) at a flow rate of 0.4 mL min−1, and acetonitrile/methanol (4:1, v/v)–0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive and negative ionization modes. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 1.097–2246 ng mL−1 for Neochlorogenic Acid, 6.535–6692 ng mL−1 for chlorogenic Acid, 2.103–2153 ng mL−1 for cryptochlorogenic Acid, 0.5058–129.5 ng mL−1 for 3,5-dicaffeoylquinic Acid, 0.3205–82.05 ng mL−1 for 3,4-dicaffeoylquinic Acid, 1.002–512.8 ng mL−1 for isoforsythoside, 0.4795–982.1 ng mL−1 for forsythoside A and 0.7587–776.9 ng mL−1 for forsythoside B, respectively. The intra- and inter-batch precisions were all within 15% and the accuracy (relative error, RE%) all ranged from 85.68% to 114.7%. It was shown from pharmacokinetic parameters that the rank order of AUC0–t, Cmax and T1/2k for phenolic Acids was chlorogenic Acid > Neochlorogenic Acid ≥ cryptochlorogenic Acid > 3,4-dicaffeoylquinic Acid ≥ 3,5-dicaffeoylquinic Acid (most of them had significant differences), which corresponded to their administration dosages to rats, but that of MRT0–t and T1/2z were opposite. Besides, the AUC0–t, Cmax, MRT and T1/2z except T1/2k of isoforsythoside and forsythoside B had no significant difference, compared to that of forsythoside A though their administration dosages were significantly lower than that of forsythoside A. All results showed that the method was applied to the pharmacokinetic study of the eight caffeic Acid derivatives in rat plasma successfully after oral administration of Flos Lonicerae–Fructus Forsythiae herb combination, and there were significant differences of caffeic Acid derivatives even isomers in the pharmacokinetic parameters.
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simultaneous determination of phenolic Acids by uplc ms ms in rat plasma and its application in pharmacokinetic study after oral administration of flos lonicerae preparations
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Wei Zhou, Wenzheng Ju, Jinjun Shan, Minxin Meng, Liuqing DiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of five phenolic Acids (Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to simultaneously determine the five phenolic Acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 ml min−1, and acetonitrile/methanol (4:1, v/v)-0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74–378 ng ml−1 for Neochlorogenic Acid, 0.50–1030 ng ml−1 for chlorogenic Acid, 1.9–250 ng ml−1 for cryptochlorogenic Acid, 0.74–380 ng ml−1 for 3,5-dicaffeoylquinic Acid, and 5.1–328 ng ml−1 for 3,4-dicaffeoylquinic Acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
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Simultaneous determination of phenolic Acids by UPLC–MS/MS in rat plasma and its application in pharmacokinetic study after oral administration of Flos Lonicerae preparations
Journal of pharmaceutical and biomedical analysis, 2013Co-Authors: Wei Zhou, Jinjun Shan, Minxin Meng, Shijia Liu, Baochang CaiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of five phenolic Acids (Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to simultaneously determine the five phenolic Acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 ml min−1, and acetonitrile/methanol (4:1, v/v)-0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74–378 ng ml−1 for Neochlorogenic Acid, 0.50–1030 ng ml−1 for chlorogenic Acid, 1.9–250 ng ml−1 for cryptochlorogenic Acid, 0.74–380 ng ml−1 for 3,5-dicaffeoylquinic Acid, and 5.1–328 ng ml−1 for 3,4-dicaffeoylquinic Acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
Michio Aitani - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC Complementary and Alternative Medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
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Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC Complementary and Alternative Medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:Background An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Methods Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). Results It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. Conclusion These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
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Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC complementary and alternative medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
Baochang Cai - One of the best experts on this subject based on the ideXlab platform.
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Study on the main components interaction from Flos Lonicerae and Fructus Forsythiae and their dissolution in vitro and intestinal absorption in rats.
PloS one, 2014Co-Authors: Wei Zhou, Jinjun Shan, Xiaobin Tan, Shouchuan Wang, Ailing Yin, Baochang CaiAbstract:The Flos Lonicerae-Fructus Forsythiae herb couple is the basic components of Chinese herbal preparations (Shuang-Huang-Lian tablet, Yin-Qiao-Jie-Du tablet and Fufang Qin-Lan oral liquid), and its pharmacological effects were significantly higher than that in Flos Lonicerae or Fructus Forsythiae, but the reasons remained unknown. In the present study, pattern recognition analysis (hierarchical cluster analysis (HCA) and principal component analysis (PCA)) combined with UHPLC-ESI/LTQ-Orbitrap MS system were performed to study the chemical constitution difference between co-decoction and mixed decoction in the term of chemistry. Besides, the pharmacokinetics in vivo and intestinal absorption in vitro combined with pattern recognition analysis were used to reveal the discrepancy between herb couple and single herbs in the view of biology. The observation from the chemical view in vitro showed that there was significant difference in quantity between co-decoction and mixed decoction by HCA, and the exposure level of isoforsythoside and 3, 5-dicaffeoylquinic Acid in co-decoction, higher than that in mixed decoction, directly resulted in the discrepancy between co-decoction and mixed decoction using both PCA and HCA. The observation from the pharmacokinetics displayed that the exposure level in vivo of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A, higher than that in single herbs, was the main factor contributing to the difference by both PCA and HCA, interestingly consistent with the results obtained from Caco-2 cells in vitro, which indicated that it was because of intestinal absorption improvement of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A that resulted in a better efficacy of herb couple than that of single herbs from the perspective of biology. The results above illustrated that caffeic Acid derivatives in Flos Lonicerae-Fructus Forsythiae herb couple could be considered as chemical markers for quality control of its preparations.
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Simultaneous determination of phenolic Acids by UPLC–MS/MS in rat plasma and its application in pharmacokinetic study after oral administration of Flos Lonicerae preparations
Journal of pharmaceutical and biomedical analysis, 2013Co-Authors: Wei Zhou, Jinjun Shan, Minxin Meng, Shijia Liu, Baochang CaiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of five phenolic Acids (Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to simultaneously determine the five phenolic Acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 ml min−1, and acetonitrile/methanol (4:1, v/v)-0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74–378 ng ml−1 for Neochlorogenic Acid, 0.50–1030 ng ml−1 for chlorogenic Acid, 1.9–250 ng ml−1 for cryptochlorogenic Acid, 0.74–380 ng ml−1 for 3,5-dicaffeoylquinic Acid, and 5.1–328 ng ml−1 for 3,4-dicaffeoylquinic Acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
Jinjun Shan - One of the best experts on this subject based on the ideXlab platform.
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Study on the main components interaction from Flos Lonicerae and Fructus Forsythiae and their dissolution in vitro and intestinal absorption in rats.
PloS one, 2014Co-Authors: Wei Zhou, Jinjun Shan, Xiaobin Tan, Shouchuan Wang, Ailing Yin, Baochang CaiAbstract:The Flos Lonicerae-Fructus Forsythiae herb couple is the basic components of Chinese herbal preparations (Shuang-Huang-Lian tablet, Yin-Qiao-Jie-Du tablet and Fufang Qin-Lan oral liquid), and its pharmacological effects were significantly higher than that in Flos Lonicerae or Fructus Forsythiae, but the reasons remained unknown. In the present study, pattern recognition analysis (hierarchical cluster analysis (HCA) and principal component analysis (PCA)) combined with UHPLC-ESI/LTQ-Orbitrap MS system were performed to study the chemical constitution difference between co-decoction and mixed decoction in the term of chemistry. Besides, the pharmacokinetics in vivo and intestinal absorption in vitro combined with pattern recognition analysis were used to reveal the discrepancy between herb couple and single herbs in the view of biology. The observation from the chemical view in vitro showed that there was significant difference in quantity between co-decoction and mixed decoction by HCA, and the exposure level of isoforsythoside and 3, 5-dicaffeoylquinic Acid in co-decoction, higher than that in mixed decoction, directly resulted in the discrepancy between co-decoction and mixed decoction using both PCA and HCA. The observation from the pharmacokinetics displayed that the exposure level in vivo of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A, higher than that in single herbs, was the main factor contributing to the difference by both PCA and HCA, interestingly consistent with the results obtained from Caco-2 cells in vitro, which indicated that it was because of intestinal absorption improvement of Neochlorogenic Acid, 3, 4-dicaffeoylquinic Acid, isoforsythoside and forsythoside A that resulted in a better efficacy of herb couple than that of single herbs from the perspective of biology. The results above illustrated that caffeic Acid derivatives in Flos Lonicerae-Fructus Forsythiae herb couple could be considered as chemical markers for quality control of its preparations.
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simultaneous determination of caffeic Acid derivatives by uplc ms ms in rat plasma and its application in pharmacokinetic study after oral administration of flos lonicerae fructus forsythiae herb combination
Journal of Chromatography B, 2014Co-Authors: Wei Zhou, Wenzheng Ju, Jinjun Shan, Minxin Meng, Shouquan Wang, Liuqing DiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of eight caffeic Acid derivatives (forsythoside A, isoforsythoside, forsythoside B, Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae–Fructus Forsythiae herb combination in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to determine the eight caffeic Acid derivatives simultaneously in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with n-butyl alcohol/ethyl acetate (7:3, v/v). The separation was performed on an Acquity UPLC HSS T3 C18 column (100 mm × 2.1 mm, 1.8 μm) at a flow rate of 0.4 mL min−1, and acetonitrile/methanol (4:1, v/v)–0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive and negative ionization modes. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 1.097–2246 ng mL−1 for Neochlorogenic Acid, 6.535–6692 ng mL−1 for chlorogenic Acid, 2.103–2153 ng mL−1 for cryptochlorogenic Acid, 0.5058–129.5 ng mL−1 for 3,5-dicaffeoylquinic Acid, 0.3205–82.05 ng mL−1 for 3,4-dicaffeoylquinic Acid, 1.002–512.8 ng mL−1 for isoforsythoside, 0.4795–982.1 ng mL−1 for forsythoside A and 0.7587–776.9 ng mL−1 for forsythoside B, respectively. The intra- and inter-batch precisions were all within 15% and the accuracy (relative error, RE%) all ranged from 85.68% to 114.7%. It was shown from pharmacokinetic parameters that the rank order of AUC0–t, Cmax and T1/2k for phenolic Acids was chlorogenic Acid > Neochlorogenic Acid ≥ cryptochlorogenic Acid > 3,4-dicaffeoylquinic Acid ≥ 3,5-dicaffeoylquinic Acid (most of them had significant differences), which corresponded to their administration dosages to rats, but that of MRT0–t and T1/2z were opposite. Besides, the AUC0–t, Cmax, MRT and T1/2z except T1/2k of isoforsythoside and forsythoside B had no significant difference, compared to that of forsythoside A though their administration dosages were significantly lower than that of forsythoside A. All results showed that the method was applied to the pharmacokinetic study of the eight caffeic Acid derivatives in rat plasma successfully after oral administration of Flos Lonicerae–Fructus Forsythiae herb combination, and there were significant differences of caffeic Acid derivatives even isomers in the pharmacokinetic parameters.
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simultaneous determination of phenolic Acids by uplc ms ms in rat plasma and its application in pharmacokinetic study after oral administration of flos lonicerae preparations
Journal of Pharmaceutical and Biomedical Analysis, 2013Co-Authors: Wei Zhou, Wenzheng Ju, Jinjun Shan, Minxin Meng, Liuqing DiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of five phenolic Acids (Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to simultaneously determine the five phenolic Acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 ml min−1, and acetonitrile/methanol (4:1, v/v)-0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74–378 ng ml−1 for Neochlorogenic Acid, 0.50–1030 ng ml−1 for chlorogenic Acid, 1.9–250 ng ml−1 for cryptochlorogenic Acid, 0.74–380 ng ml−1 for 3,5-dicaffeoylquinic Acid, and 5.1–328 ng ml−1 for 3,4-dicaffeoylquinic Acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
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Simultaneous determination of phenolic Acids by UPLC–MS/MS in rat plasma and its application in pharmacokinetic study after oral administration of Flos Lonicerae preparations
Journal of pharmaceutical and biomedical analysis, 2013Co-Authors: Wei Zhou, Jinjun Shan, Minxin Meng, Shijia Liu, Baochang CaiAbstract:Abstract The current study aims to investigate the pharmacokinetic study of five phenolic Acids (Neochlorogenic Acid, chlorogenic Acid, cryptochlorogenic Acid, 3,5-dicaffeoylquinic Acid and 3,4-dicaffeoylquinic Acid) following oral administration of Flos Lonicerae preparations in rats. A rapid and sensitive ultra performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed to simultaneously determine the five phenolic Acids in rat plasma. After mixing with the internal standard (IS) tinidazole, plasma samples were pretreated by liquid–liquid extraction with ethyl acetate/n-hexane (9:1, v/v). The separation was performed on an Acquity UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) at a flow rate of 0.4 ml min−1, and acetonitrile/methanol (4:1, v/v)-0.4% formic Acid was used as mobile phase. The detection was performed on a triple quadrupole tandem mass spectrometer by multiple reaction monitoring (MRM) via electrospray ionization (ESI) source with positive ionization mode. All calibration curves had good linearity (r > 0.991) over the concentration ranges of 0.74–378 ng ml−1 for Neochlorogenic Acid, 0.50–1030 ng ml−1 for chlorogenic Acid, 1.9–250 ng ml−1 for cryptochlorogenic Acid, 0.74–380 ng ml−1 for 3,5-dicaffeoylquinic Acid, and 5.1–328 ng ml−1 for 3,4-dicaffeoylquinic Acid. The intra-and inter-day precision were within 15% and the accuracy ranged from 86.2% to 114.1%.
Hiroshi Shimoda - One of the best experts on this subject based on the ideXlab platform.
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inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC Complementary and Alternative Medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
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Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC Complementary and Alternative Medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:Background An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Methods Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). Results It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. Conclusion These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
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Inhibitory effect of green coffee bean extract on fat accumulation and body weight gain in mice
BMC complementary and alternative medicine, 2006Co-Authors: Hiroshi Shimoda, Emi Seki, Michio AitaniAbstract:An epidemiological study conducted in Italy indicated that coffee has the greatest antioxidant capacity among the commonly consumed beverages. Green coffee bean is rich in chlorogenic Acid and its related compounds. The effect of green coffee bean extract (GCBE) on fat accumulation and body weight in mice was assessed with the objective of investigating the effect of GCBE on mild obesity. Male ddy mice were fed a standard diet containing GCBE and its principal constituents, namely, caffeine and chlorogenic Acid, for 14 days. Further, hepatic triglyceride (TG) level was also investigated after consecutive administration (13 days) of GCBE and its constituents. To examine the effect of GCBE and its constituents on fat absorption, serum TG changes were evaluated in olive oil-loaded mice. In addition, to investigate the effect on hepatic TG metabolism, carnitine palmitoyltransferase (CPT) activity in mice was evaluated after consecutive ingestion (6 days) of GCBE and its constituents (caffeine, chlorogenic Acid, Neochlorogenic Acid and feruloylquinic Acid mixture). It was found that 0.5% and 1% GCBE reduced visceral fat content and body weight. Caffeine and chlorogenic Acid showed a tendency to reduce visceral fat and body weight. Oral administration of GCBE (100 and 200 mg/kg· day) for 13 days showed a tendency to reduce hepatic TG in mice. In the same model, chlorogenic Acid (60 mg/kg· day) reduced hepatic TG level. In mice loaded with olive oil (5 mL/kg), GCBE (200 and 400 mg/kg) and caffeine (20 and 40 mg/kg) reduced serum TG level. GCBE (1%), Neochlorogenic Acid (0.028% and 0.055%) and feruloylquinic Acid mixture (0.081%) significantly enhanced hepatic CPT activity in mice. However, neither caffeine nor chlorogenic Acid alone was found to enhance CPT activity. These results suggest that GCBE is possibly effective against weight gain and fat accumulation by inhibition of fat absorption and activation of fat metabolism in the liver. Caffeine was found to be a suppressor of fat absorption, while chlorogenic Acid was found to be partially involved in the suppressive effect of GCBE that resulted in the reduction of hepatic TG level. Phenolic compounds such as Neochlorogenic Acid and feruloylquinic Acid mixture, except chlorogenic Acid, can enhance hepatic CPT activity.
