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Senne Dillen - One of the best experts on this subject based on the ideXlab platform.
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nutrient dependent control oF short Neuropeptide F transcript levels via components oF the insulin igF signaling pathway in the desert locust schistocerca gregaria
Insect Biochemistry and Molecular Biology, 2016Co-Authors: Senne Dillen, Ziwei ChenAbstract:Peptides oF the short Neuropeptide F (sNPF) Family modulate Feeding behavior in a wide variety oF insect species, including the desert locust, Schistocerca gregaria. Likewise, the nutritional state oF the animal can strongly aFFect sNPF expression. Although several studies have been published describing these nutrient-dependent eFFects, it remains largely unclear how they are achieved. In this study, we describe a series oF in vivo experiments which indicate that it is not the act oF Feeding in se, but rather the consequent availability oF nutrients in the insect's hemolymph that gives rise to the postprandial modulation oF sNPF expression. Furthermore, by perForming a series oF RNAi-mediated knockdown experiments, we were able to show that components oF the evolutionarily conserved insulin/insulin-related growth Factor (IGF) signaling pathway Form a Functional link between nutrient levels and sNPF transcript levels.
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identiFication oF the short Neuropeptide F precursor in the desert locust evidence For an inhibitory role oF snpF in the control oF Feeding
Peptides, 2014Co-Authors: Senne Dillen, Sven Zels, Rik Verdonck, Pieter Van WielendaeleAbstract:Peptides oF the short Neuropeptide F (sNPF) Family have been shown to modulate Feeding behavior in a wide variety oF insect species. While these peptides stimulate Feeding and Food-searching behavior in Drosophila melanogaster and Apis melliFera, an opposite eFFect has recently been demonstrated in the desert locust, Schistocerca gregaria. In this study, we elaborate on these observations with the identiFication oF the nucleotide sequence encoding the Schgr-sNPF precursor and the study oF its role in the regulation oF locust Feeding behavior. We conFirm that both Schgr-sNPF-like peptides, previously identiFied in mass spectrometric studies, are genuine precursor-encoded peptides. RNA interFerence mediated silencing oF the Schgr-sNPF precursor transcript generates novel evidence For an inhibitory role oF Schgr-sNPF in the regulation oF Feeding in S. gregaria. Furthermore, we show that starvation reduces the Schgr-sNPF precursor transcript level in the optic lobes, the primary visual centers oF the locust brain. Our data indicate that Schgr-sNPF exerts an inhibitory eFFect on Food uptake in the desert locust, which contrasts with eFFects oF sNPF reported For several other insect species.
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In vivo eFFect oF Neuropeptide F on ecdysteroidogenesis in adult Female desert locusts (Schistocerca gregaria).
Journal of insect physiology, 2013Co-Authors: Pieter Van Wielendaele, Niels Wynant, Senne Dillen, Liesbeth Badisco, Elisabeth Marchal, Jozef Vanden BroeckAbstract:Neuropeptides are important regulatory Factors that mediate key liFe processes, both in vertebrates and invertebrates. Many insect Neuropeptides display pleiotropic activities, which means that they can inFluence multiple aspects oF insect physiology. In the Fruit Fly, Drosophila melanogaster, Neuropeptide F (NPF) mediates diverse physiological processes, such as learning, stress responses, Feeding and male courtship behavior. In locusts, only a truncated Form oF the predicted "Full-length" NPF, the nonapeptide "trNPF", has been isolated. This nonapeptide previously proved to be biologically active, since it was shown to inFluence Food intake and weight increase, as well as oocyte growth in adult Female desert locusts (Schistocerca gregaria [Forskål]). In the present study, we have Further analyzed the eFFect oF trNPF on Female reproductive physiology in S. gregaria. We conFirmed that daily trNPF injections in adult Females elicit an increase oF oocyte size. In addition, an RNAi-mediated knockdown oF the Schgr-NPF precursor transcript in adult Female locusts resulted in the opposite eFFect, i.e. signiFicantly smaller oocytes. Moreover, we discovered that daily injections oF trNPF in adult Female S. gregaria, caused higher ecdysteroid titers in the ovaries and accelerated the appearance oF ecdysteroid peaks in the hemolymph oF these animals. The RNAi-based knockdown oF the Schgr-NPF precursor transcript clearly resulted in reduction oF both hemolymph and ovarian ecdysteroid concentrations, conFirming the stimulatory eFFects oF trNPF injections on adult Female ecdysteroid levels. The observed results are discussed in relation to previous reports on NPF activities in locusts and other insects.
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Neuropeptide F regulates male reproductive processes in the desert locust, Schistocerca gregaria.
Insect Biochemistry and Molecular Biology, 2013Co-Authors: Pieter Van Wielendaele, Niels Wynant, Senne Dillen, Sven Zels, Liesbeth Badisco, Jozef Vanden BroeckAbstract:Although Neuropeptide F (NPF) has been identiFied in diFFerent insect species, its Function has mainly been studied in the Fruit Fly, Drosophila melanogaster, where it regulates diverse physiological processes, such as learning, stress responses and male courtship behavior. In locusts, only a truncated Form oF the “Full-length” NPF (the biologically active “trNPF”) has been isolated. This 9 AA peptide stimulates oocyte maturation, Food intake and weight increase in adult desert locusts (Schistocerca gregaria [Forskal]). In this study, we investigated whether this peptide is also involved in the regulation oF male reproductive physiology in this orthopteran species. Daily injections oF trNPF in adult males resulted in proportionally heavier testes and seminal vesicles, while RNAi-mediated knockdown oF the Schgr-NPF precursor transcript gave rise to proportionally lighter testes and seminal vesicles. Furthermore, adult males precociously displayed courtship behavior when injected daily with trNPF, while this behavior was inhibited or delayed by RNAi knockdown oF the Schgr-NPF precursor transcript. In order to Further analyze these eFFects oF trNPF on male reproductive physiology, Fertility oF males was tested by analyzing progeny numbers Following copulation with untreated Females. In this way, we showed that daily trNPF injection in adult males resulted in a larger egg pod size and a higher percentage oF hatched eggs per egg pod aFter copulation, while RNAi knockdown caused the opposite eFFects. Taken together, we provide clear evidence For a role oF trNPF in the regulation oF reproductive physiology in adult males oF the desert locust, S. gregaria. Possible modes oF action oF trNPF in inFluencing these reproductive processes in male locusts are discussed.
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Functional characterization oF the short Neuropeptide F receptor in the desert locust schistocerca gregaria
PLOS ONE, 2013Co-Authors: Senne Dillen, Sven Zels, Heleen Verlinden, Jornt Spit, Pieter Van WielendaeleAbstract:Whereas short Neuropeptide F (sNPF) has already been reported to stimulate Feeding behaviour in a variety oF insect species, the opposite eFFect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA From the desert locust, Schistocerca gregaria. Cell-based Functional analysis oF this receptor indicated that it is activated by both known isoForms oF Schgr-sNPF in a concentration dependent manner, with EC50 values in the nanomolar range. This Schgr-sNPF receptor constitutes the First Functionally characterized peptide GPCR in locusts. The in vivo eFFects oF the sNPF signalling pathway on the regulation oF Feeding in locusts were Further studied by knocking down the newly identiFied Schgr-sNPF receptor by means oF RNA interFerence, as well as by means oF peptide injection studies. While injection oF sNPF caused an inhibitory eFFect on Food uptake in the desert locust, knocking down the corresponding peptide receptor resulted in an increase oF total Food uptake when compared to control animals. This is the First comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and Feeding, prompting a reconsideration oF the diverse roles oF sNPFs in the physiology oF insects.
Pieter Van Wielendaele - One of the best experts on this subject based on the ideXlab platform.
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identiFication oF the short Neuropeptide F precursor in the desert locust evidence For an inhibitory role oF snpF in the control oF Feeding
Peptides, 2014Co-Authors: Senne Dillen, Sven Zels, Rik Verdonck, Pieter Van WielendaeleAbstract:Peptides oF the short Neuropeptide F (sNPF) Family have been shown to modulate Feeding behavior in a wide variety oF insect species. While these peptides stimulate Feeding and Food-searching behavior in Drosophila melanogaster and Apis melliFera, an opposite eFFect has recently been demonstrated in the desert locust, Schistocerca gregaria. In this study, we elaborate on these observations with the identiFication oF the nucleotide sequence encoding the Schgr-sNPF precursor and the study oF its role in the regulation oF locust Feeding behavior. We conFirm that both Schgr-sNPF-like peptides, previously identiFied in mass spectrometric studies, are genuine precursor-encoded peptides. RNA interFerence mediated silencing oF the Schgr-sNPF precursor transcript generates novel evidence For an inhibitory role oF Schgr-sNPF in the regulation oF Feeding in S. gregaria. Furthermore, we show that starvation reduces the Schgr-sNPF precursor transcript level in the optic lobes, the primary visual centers oF the locust brain. Our data indicate that Schgr-sNPF exerts an inhibitory eFFect on Food uptake in the desert locust, which contrasts with eFFects oF sNPF reported For several other insect species.
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In vivo eFFect oF Neuropeptide F on ecdysteroidogenesis in adult Female desert locusts (Schistocerca gregaria).
Journal of insect physiology, 2013Co-Authors: Pieter Van Wielendaele, Niels Wynant, Senne Dillen, Liesbeth Badisco, Elisabeth Marchal, Jozef Vanden BroeckAbstract:Neuropeptides are important regulatory Factors that mediate key liFe processes, both in vertebrates and invertebrates. Many insect Neuropeptides display pleiotropic activities, which means that they can inFluence multiple aspects oF insect physiology. In the Fruit Fly, Drosophila melanogaster, Neuropeptide F (NPF) mediates diverse physiological processes, such as learning, stress responses, Feeding and male courtship behavior. In locusts, only a truncated Form oF the predicted "Full-length" NPF, the nonapeptide "trNPF", has been isolated. This nonapeptide previously proved to be biologically active, since it was shown to inFluence Food intake and weight increase, as well as oocyte growth in adult Female desert locusts (Schistocerca gregaria [Forskål]). In the present study, we have Further analyzed the eFFect oF trNPF on Female reproductive physiology in S. gregaria. We conFirmed that daily trNPF injections in adult Females elicit an increase oF oocyte size. In addition, an RNAi-mediated knockdown oF the Schgr-NPF precursor transcript in adult Female locusts resulted in the opposite eFFect, i.e. signiFicantly smaller oocytes. Moreover, we discovered that daily injections oF trNPF in adult Female S. gregaria, caused higher ecdysteroid titers in the ovaries and accelerated the appearance oF ecdysteroid peaks in the hemolymph oF these animals. The RNAi-based knockdown oF the Schgr-NPF precursor transcript clearly resulted in reduction oF both hemolymph and ovarian ecdysteroid concentrations, conFirming the stimulatory eFFects oF trNPF injections on adult Female ecdysteroid levels. The observed results are discussed in relation to previous reports on NPF activities in locusts and other insects.
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Functional characterization oF the short Neuropeptide F receptor in the desert locust schistocerca gregaria
PLOS ONE, 2013Co-Authors: Senne Dillen, Sven Zels, Heleen Verlinden, Jornt Spit, Pieter Van WielendaeleAbstract:Whereas short Neuropeptide F (sNPF) has already been reported to stimulate Feeding behaviour in a variety oF insect species, the opposite eFFect was observed in the desert locust. In the present study, we cloned a G protein-coupled receptor (GPCR) cDNA From the desert locust, Schistocerca gregaria. Cell-based Functional analysis oF this receptor indicated that it is activated by both known isoForms oF Schgr-sNPF in a concentration dependent manner, with EC50 values in the nanomolar range. This Schgr-sNPF receptor constitutes the First Functionally characterized peptide GPCR in locusts. The in vivo eFFects oF the sNPF signalling pathway on the regulation oF Feeding in locusts were Further studied by knocking down the newly identiFied Schgr-sNPF receptor by means oF RNA interFerence, as well as by means oF peptide injection studies. While injection oF sNPF caused an inhibitory eFFect on Food uptake in the desert locust, knocking down the corresponding peptide receptor resulted in an increase oF total Food uptake when compared to control animals. This is the First comprehensive study in which a clearly negative correlation is described between the sNPF signalling pathway and Feeding, prompting a reconsideration oF the diverse roles oF sNPFs in the physiology oF insects.
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regulation oF Feeding by Neuropeptide F in the desert locust schistocerca gregaria
Insect Biochemistry and Molecular Biology, 2013Co-Authors: Pieter Van Wielendaele, Senne Dillen, Sven Zels, Liesbeth BadiscoAbstract:Our knowledge on the physiological Function oF the insect Neuropeptide F (NPF) mostly comes From studies in the Fruit Fly, Drosophila melanogaster, where NPF was shown to regulate diverse processes, such as Feeding, learning and responding to stress. In the desert locust, Schistocerca gregaria, only a truncated Form oF the “Full-length” NPF (the biologically active “trNPF”) has been isolated. In this study, we investigated whether this peptide is involved in the regulation oF Feeding in this orthopteran species. In the S. gregaria EST-database, an NPF-precursor encoding transcript was Found. Alignment with other insect NPF-precursors showed relatively highest sequence conservation within the trNPF region (and the Flanking dibasic cleavage site), as compared to other regions oF the NPF-precursor. Quantitative real-time RT-PCR revealed that the Schgr-NPF-precursor encoding transcript occurs throughout the central nervous system with relatively high transcript levels in the brain, optic lobes and suboesophageal ganglion. It was also detected at relatively high levels in the midgut, which suggests that the encoded peptide also Functions in the digestive system. Moreover, Schgr-NPF-transcript levels were notably higher in starved animals than in animals Fed ad libitum, while transcript levels were also shown to be regulated aFter the consumption oF a meal. Injection oF locust trNPF in adults stimulated Food intake, while RNAi knockdown reduced Food intake. Furthermore, injection oF trNPF in adults stimulated weight increase, while RNAi knockdown reduced weight gain. This eFFect oF trNPF on body weight gain may result From its stimulatory eFFect on Food intake. Taken together, we provide clear evidence For an important role oF trNPF in the regulation oF Feeding in the desert locust, S. gregaria.
Sven Zels - One of the best experts on this subject based on the ideXlab platform.
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Regulation oF Feeding and Metabolism by Neuropeptide F and Short Neuropeptide F in Invertebrates.
Frontiers in endocrinology, 2019Co-Authors: Melissa Fadda, Sven Zels, Isabel Beets, Ilayda Hasakiogullari, Liesbet Temmerman, Liliane SchoofsAbstract:Numerous Neuropeptide systems have been implicated to coordinately control energy homeostasis, both centrally and peripherally. However, the vertebrate Neuropeptide Y (NPY) system has emerged as the best described one regarding this biological process. The protostomian ortholog oF NPY is Neuropeptide F, characterized by an RXRF(Y)amide carboxyterminal motiF. A second Neuropeptide system is short NPF, characterized by an M/T/L/FRF(W)amide carboxyterminal motiF. Although both short and long NPF Neuropeptide systems display carboxyterminal sequence similarities, they are evolutionary distant and likely already arose as separate signaling systems in the common ancestor oF deuterostomes and protostomes, indicating the Functional importance oF both. Both NPF and short-NPF systems seem to have roles in the coordination oF Feeding across bilaterian species, but during chordate evolution, the short NPF system appears to have been lost or evolved into the prolactin releasing peptide signaling system, which regulates Feeding and has been suggested to be orthologous to sNPF. Here we review the roles oF both NPF and sNPF systems in the regulation oF Feeding and metabolism in invertebrates.
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Table_1_Regulation oF Feeding and Metabolism by Neuropeptide F and Short Neuropeptide F in Invertebrates.docx
2019Co-Authors: Melissa Fadda, Sven Zels, Isabel Beets, Ilayda Hasakiogullari, Liesbet Temmerman, Liliane SchoofsAbstract:Numerous Neuropeptide systems have been implicated to coordinately control energy homeostasis, both centrally and peripherally. However, the vertebrate Neuropeptide Y (NPY) system has emerged as the best described one regarding this biological process. The protostomian ortholog oF NPY is Neuropeptide F, characterized by an RXRF(Y)amide carboxyterminal motiF. A second Neuropeptide system is short NPF, characterized by an M/T/L/FRF(W)amide carboxyterminal motiF. Although both short and long NPF Neuropeptide systems display carboxyterminal sequence similarities, they are evolutionary distant and likely already arose as separate signaling systems in the common ancestor oF deuterostomes and protostomes, indicating the Functional importance oF both. Both NPF and short-NPF systems seem to have roles in the coordination oF Feeding across bilaterian species, but during chordate evolution, the short NPF system appears to have been lost or evolved into the prolactin releasing peptide signaling system, which regulates Feeding and has been suggested to be orthologous to sNPF. Here we review the roles oF both NPF and sNPF systems in the regulation oF Feeding and metabolism in invertebrates.
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characterization oF a Neuropeptide F receptor in the tsetse Fly glossina morsitans morsitans
Journal of Insect Physiology, 2016Co-Authors: Jelle Caers, Sven Zels, Jan Van Den Abbeele, Matthias B Van Hiel, Katleen Peymen, Liesbeth Van Rompay, Liliane Schoofs, Isabel BeetsAbstract:Neuropeptides related to mammalian Neuropeptide Y (NPY) and insect Neuropeptide F (NPF) are conserved throughout Metazoa and intimately involved in a wide range oF biological processes. In insects NPF is involved in regulating Feeding, learning, stress and reproductive behavior. Here we identiFied and characterized an NPF receptor oF the tsetse Fly, Glossina morsitans morsitans, the sole transmitter oF Trypanosoma parasites causing sleeping sickness. We isolated cDNA sequences encoding tsetse NPF (Glomo-NPF) and its receptor (Glomo-NPFR), and examined their spatial and temporal expression patterns using quantitative PCR. In tsetse Flies, npFr transcripts are expressed throughout development and most abundantly in the central nervous system, whereas low expression is Found in the Flight muscles and posterior midgut. Expression oF npF, by contrast, shows low transcript levels during development but is strongly expressed in the posterior midgut and brain oF adult Flies. Expression oF Glomo-npF and its receptor in the brain and digestive system suggests that NPF may have conserved neuromodulatory or hormonal Functions in tsetse Flies, such as in the regulation oF Feeding behavior. Cell-based activity studies oF the Glomo-NPFR showed that Glomo-NPF activates the receptor up to nanomolar concentrations. The molecular data oF Glomo-NPF and Glomo-NPFR paves the way For Further investigation oF its Functions in tsetse Flies.
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identiFication oF the short Neuropeptide F precursor in the desert locust evidence For an inhibitory role oF snpF in the control oF Feeding
Peptides, 2014Co-Authors: Senne Dillen, Sven Zels, Rik Verdonck, Pieter Van WielendaeleAbstract:Peptides oF the short Neuropeptide F (sNPF) Family have been shown to modulate Feeding behavior in a wide variety oF insect species. While these peptides stimulate Feeding and Food-searching behavior in Drosophila melanogaster and Apis melliFera, an opposite eFFect has recently been demonstrated in the desert locust, Schistocerca gregaria. In this study, we elaborate on these observations with the identiFication oF the nucleotide sequence encoding the Schgr-sNPF precursor and the study oF its role in the regulation oF locust Feeding behavior. We conFirm that both Schgr-sNPF-like peptides, previously identiFied in mass spectrometric studies, are genuine precursor-encoded peptides. RNA interFerence mediated silencing oF the Schgr-sNPF precursor transcript generates novel evidence For an inhibitory role oF Schgr-sNPF in the regulation oF Feeding in S. gregaria. Furthermore, we show that starvation reduces the Schgr-sNPF precursor transcript level in the optic lobes, the primary visual centers oF the locust brain. Our data indicate that Schgr-sNPF exerts an inhibitory eFFect on Food uptake in the desert locust, which contrasts with eFFects oF sNPF reported For several other insect species.
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Neuropeptide F regulates male reproductive processes in the desert locust, Schistocerca gregaria.
Insect Biochemistry and Molecular Biology, 2013Co-Authors: Pieter Van Wielendaele, Niels Wynant, Senne Dillen, Sven Zels, Liesbeth Badisco, Jozef Vanden BroeckAbstract:Although Neuropeptide F (NPF) has been identiFied in diFFerent insect species, its Function has mainly been studied in the Fruit Fly, Drosophila melanogaster, where it regulates diverse physiological processes, such as learning, stress responses and male courtship behavior. In locusts, only a truncated Form oF the “Full-length” NPF (the biologically active “trNPF”) has been isolated. This 9 AA peptide stimulates oocyte maturation, Food intake and weight increase in adult desert locusts (Schistocerca gregaria [Forskal]). In this study, we investigated whether this peptide is also involved in the regulation oF male reproductive physiology in this orthopteran species. Daily injections oF trNPF in adult males resulted in proportionally heavier testes and seminal vesicles, while RNAi-mediated knockdown oF the Schgr-NPF precursor transcript gave rise to proportionally lighter testes and seminal vesicles. Furthermore, adult males precociously displayed courtship behavior when injected daily with trNPF, while this behavior was inhibited or delayed by RNAi knockdown oF the Schgr-NPF precursor transcript. In order to Further analyze these eFFects oF trNPF on male reproductive physiology, Fertility oF males was tested by analyzing progeny numbers Following copulation with untreated Females. In this way, we showed that daily trNPF injection in adult males resulted in a larger egg pod size and a higher percentage oF hatched eggs per egg pod aFter copulation, while RNAi knockdown caused the opposite eFFects. Taken together, we provide clear evidence For a role oF trNPF in the regulation oF reproductive physiology in adult males oF the desert locust, S. gregaria. Possible modes oF action oF trNPF in inFluencing these reproductive processes in male locusts are discussed.
Prasert Sobhon - One of the best experts on this subject based on the ideXlab platform.
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Distribution oF Neuropeptide F in the ventral nerve cord and its possible role on testicular development and germ cell proliFeration in the giant Freshwater prawn, Macrobrachium rosenbergii
Cell and Tissue Research, 2019Co-Authors: Sirorat Thongrod, Chaitip Wanichanon, Prasert SobhonAbstract:Neuropeptide F in invertebrates is a homolog oF Neuropeptide Y in mammals and it is a member oF FMRFamide-related peptides. In arthropods, such as insects, there are two types oF Neuropeptide F comprising long Neuropeptide F (NPF) and short Neuropeptide F (sNPF). Both NPFs are known to play a crucial role in the regulations oF Foraging, Feeding-related behaviors, circadian rhythm, stress responses, aggression and reproduction in invertebrates. We have earlier Found that in the giant Freshwater prawn, Macrobrachium rosenbergii , there are three isoForms oF NPF and Four isoForms oF sNPF and that NPFs are expressed in the eyestalks and brain. In the present study, we investigate Further the tissue distribution oF NPF-I in the ventral nerve cord (VNC) and its role in the development oF testes in small male (SM) Macrobrachium rosenbergii . By immunolocalization, using the rabbit polyclonal antibody against NPF-I as a probe, we could detect NPF-I immunoreactivity in the neuropils and neuronal clusters oF the subesophageal ganglia (SEG), thoracic ganglia (TG) and abdominal ganglia (AG) oF the SM prawns. In Functional assays, the administrations oF synthetic NPF-I (KPDPTQLAAMADALKYLQELDKYYSQVSRPRFamide) and sNPF (APALRLRFamide) peptides signiFicantly increased the growth rates oF SM prawns and signiFicantly increased the gonadosomatic index (GSI) and proliFerations oF early germ cells in the seminiFerous tubules oF their testes. It is, thereFore, suggestive that NPFs may play critical roles in energy homeostasis towards promoting growth as well as testicular development in prawns that could be applied in the aquaculture oF this species.
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Neuropeptide F stimulates ovarian development and spawning in the Female giant Freshwater prawn macrobrachium rosenbergii and its expression in the ovary during ovarian maturation cycle
Aquaculture, 2017Co-Authors: Yotsawan Tinikul, Sirorat Thongrod, Prasert Sobhon, Thanapong Kruangkum, Attakorn Engsusophon, Ruchanok TinikulAbstract:Abstract Neuropeptide F (NPF) plays important roles in the regulation oF a variety oF physiological processes, including stimulation oF Food intake. At present, there is a current lack oF basic knowledge regarding the eFFect oF NPF in the control oF Female crustacean reproduction. In this study, we investigated expression oF NPF in ovaries oF Female Macrobrachium rosenbergii (Mr) during ovarian development cycle, and its eFFects on ovarian maturation and spawning. MrNPF-immunoreactivity (-ir) was detected in the ovaries and its levels Fluctuated during the ovarian maturation cycle: MrNPF-ir was more intense in the early- and mid-stages oF ovarian development, and suddenly decreased in the mature stages. By RT-PCR, MrNPF transcript levels also increased during the early- and middle stages oF ovarian development, and suddenly Fell at late stage. The prawns were treated with varied doses oF MrNPF and the eFFects were investigated by assessing the ovarian maturation period, oocyte diameter (OD), gonado-somatic index (GSI), and hemolymph vitellogenin (Vg) concentration. MrNPF-treated prawns showed signiFicantly decreased ovarian maturation periods, and increased GSI, OD, and Vg levels compared with those oF control groups. MrNPF also caused signiFicantly earlier spawning compared with the control groups, while the mean Fecundity between MrNPF-treated prawns and controls did not diFFer signiFicantly. However, the numbers oF eggs per spawn among experimental groups were higher compared with those oF the controls. This work provides a novel and useFul basic knowledge oF NPF on Female reproduction in crustaceans, and its possible use in aquaculture For accelerating and enhancing reproduction oF the Female prawns. Statement oF relevance Alternative techniques to eyestalk ablation to stimulate ovarian maturation in captive prawn broodstock are needed in order to avoid cruel practice on cultured prawns. Attempts have been made in using neurohormones, including Neuropeptides to prime Female broodstocks to stimulate growth and reproduction to replace the eyestalk ablation. NPF is a Neuropeptide known For its control oF Feeding; however, there is currently no report on the role oF NPF in Female M. rosenbergii reproduction. We are the First to report the eFFects oF NPF on ovarian maturation and spawning, and its expression and Fluctuation in ovaries during the ovarian cycle in the Female decapod crustacean. Our Findings are novel and the data obtained could be useFul For accelerating and enhancing reproduction oF the Female Freshwater prawns.
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Tissue-speciFic expression oF Macrobrachium rosenbergii Neuropeptide genes, using RT-PCR.
2015Co-Authors: Saowaros Suwansa-ard, Tipsuda Thongbuakaew, Tianfang Wang, Min Zhao, Abigail Elizur, Peter J. Hanna, Prapee Sretarugsa, Scott F. Cummins, Prasert SobhonAbstract:Expression oF 11 Neuropeptide genes using gene-speciFic primers, as well as the β-actin gene. PCR used cDNA derived From 13 tissues oF Female (including 4 stages oF ovarian tissue) and 1 tissue oF male (testis). Negative control represents no cDNA in PCR. CCAP, Crustacean cardioactive peptide; CLDH, Calcitonin-like diuretic hormone; EH, Eclosion hormone; NPF, Neuropeptide F; SK, SulFakinin; SIF, SIFamide; NP, Neuroparsin.
Liliane Schoofs - One of the best experts on this subject based on the ideXlab platform.
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Regulation oF Feeding and Metabolism by Neuropeptide F and Short Neuropeptide F in Invertebrates.
Frontiers in endocrinology, 2019Co-Authors: Melissa Fadda, Sven Zels, Isabel Beets, Ilayda Hasakiogullari, Liesbet Temmerman, Liliane SchoofsAbstract:Numerous Neuropeptide systems have been implicated to coordinately control energy homeostasis, both centrally and peripherally. However, the vertebrate Neuropeptide Y (NPY) system has emerged as the best described one regarding this biological process. The protostomian ortholog oF NPY is Neuropeptide F, characterized by an RXRF(Y)amide carboxyterminal motiF. A second Neuropeptide system is short NPF, characterized by an M/T/L/FRF(W)amide carboxyterminal motiF. Although both short and long NPF Neuropeptide systems display carboxyterminal sequence similarities, they are evolutionary distant and likely already arose as separate signaling systems in the common ancestor oF deuterostomes and protostomes, indicating the Functional importance oF both. Both NPF and short-NPF systems seem to have roles in the coordination oF Feeding across bilaterian species, but during chordate evolution, the short NPF system appears to have been lost or evolved into the prolactin releasing peptide signaling system, which regulates Feeding and has been suggested to be orthologous to sNPF. Here we review the roles oF both NPF and sNPF systems in the regulation oF Feeding and metabolism in invertebrates.
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Table_1_Regulation oF Feeding and Metabolism by Neuropeptide F and Short Neuropeptide F in Invertebrates.docx
2019Co-Authors: Melissa Fadda, Sven Zels, Isabel Beets, Ilayda Hasakiogullari, Liesbet Temmerman, Liliane SchoofsAbstract:Numerous Neuropeptide systems have been implicated to coordinately control energy homeostasis, both centrally and peripherally. However, the vertebrate Neuropeptide Y (NPY) system has emerged as the best described one regarding this biological process. The protostomian ortholog oF NPY is Neuropeptide F, characterized by an RXRF(Y)amide carboxyterminal motiF. A second Neuropeptide system is short NPF, characterized by an M/T/L/FRF(W)amide carboxyterminal motiF. Although both short and long NPF Neuropeptide systems display carboxyterminal sequence similarities, they are evolutionary distant and likely already arose as separate signaling systems in the common ancestor oF deuterostomes and protostomes, indicating the Functional importance oF both. Both NPF and short-NPF systems seem to have roles in the coordination oF Feeding across bilaterian species, but during chordate evolution, the short NPF system appears to have been lost or evolved into the prolactin releasing peptide signaling system, which regulates Feeding and has been suggested to be orthologous to sNPF. Here we review the roles oF both NPF and sNPF systems in the regulation oF Feeding and metabolism in invertebrates.
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characterization oF a Neuropeptide F receptor in the tsetse Fly glossina morsitans morsitans
Journal of Insect Physiology, 2016Co-Authors: Jelle Caers, Sven Zels, Jan Van Den Abbeele, Matthias B Van Hiel, Katleen Peymen, Liesbeth Van Rompay, Liliane Schoofs, Isabel BeetsAbstract:Neuropeptides related to mammalian Neuropeptide Y (NPY) and insect Neuropeptide F (NPF) are conserved throughout Metazoa and intimately involved in a wide range oF biological processes. In insects NPF is involved in regulating Feeding, learning, stress and reproductive behavior. Here we identiFied and characterized an NPF receptor oF the tsetse Fly, Glossina morsitans morsitans, the sole transmitter oF Trypanosoma parasites causing sleeping sickness. We isolated cDNA sequences encoding tsetse NPF (Glomo-NPF) and its receptor (Glomo-NPFR), and examined their spatial and temporal expression patterns using quantitative PCR. In tsetse Flies, npFr transcripts are expressed throughout development and most abundantly in the central nervous system, whereas low expression is Found in the Flight muscles and posterior midgut. Expression oF npF, by contrast, shows low transcript levels during development but is strongly expressed in the posterior midgut and brain oF adult Flies. Expression oF Glomo-npF and its receptor in the brain and digestive system suggests that NPF may have conserved neuromodulatory or hormonal Functions in tsetse Flies, such as in the regulation oF Feeding behavior. Cell-based activity studies oF the Glomo-NPFR showed that Glomo-NPF activates the receptor up to nanomolar concentrations. The molecular data oF Glomo-NPF and Glomo-NPFR paves the way For Further investigation oF its Functions in tsetse Flies.
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molecular characterization oF a short Neuropeptide F signaling system in the tsetse Fly glossina morsitans morsitans
General and Comparative Endocrinology, 2016Co-Authors: Jelle Caers, Jan Van Den Abbeele, Matthias B Van Hiel, Katleen Peymen, Liesbeth Van Rompay, Liliane Schoofs, Isabel BeetsAbstract:Neuropeptides oF the short Neuropeptide F (sNPF) Family are widespread among arthropods and Found in every sequenced insect genome so Far. Functional studies have mainly Focused on the regulatory role oF sNPF in Feeding behavior, although this Neuropeptide Family has pleiotropic eFFects including in the control oF locomotion, osmotic homeostasis, sleep, learning and memory. Here, we set out to characterize and determine possible roles oF sNPF signaling in the haematophagous tsetse Fly Glossina morsitans morsitans, a vector oF AFrican Trypanosoma parasites causing human and animal AFrican trypanosomiasis. We cloned the G. m. morsitans cDNA sequences oF an sNPF-like receptor (Glomo-sNPFR) and precursor protein encoding Four Glomo-sNPF Neuropeptides. All Four Glomo-sNPF peptides concentration-dependently activated Glomo-sNPFR in a cell-based calcium mobilization assay, with EC50 values in the nanomolar range. Gene expression proFiles in adult Female tsetse Flies indicate that the Glomo-sNPF system is mainly restricted to the nervous system. Glomo-snpFr transcripts were also detected in the hindgut oF adult Females. In contrast to the Drosophila sNPF system, tsetse larvae lack expression oF Glomo-snpF and Glomo-snpFr genes. While Glomo-snpF transcript levels are upregulated in pupae, the onset oF Glomo-snpFr expression is delayed to adulthood. Expression proFiles in adult tissues are similar to those in other insects suggesting that the tsetse sNPF system may have similar Functions such as a regulatory role in Feeding behavior, together with a possible involvement oF sNPFR signaling in osmotic homeostasis. Our molecular data will enable Further investigations into the Functions oF sNPF signaling in tsetse Flies.
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Functional characterization oF a short Neuropeptide F-related receptor in a lophotrochozoan, the mollusk Crassostrea gigas
Journal of Experimental Biology, 2014Co-Authors: Laetitia Bigot, Liliane Schoofs, Isabel Beets, Marie-pierre Dubos, Pierre Boudry, Pascal FavrelAbstract:Members oF the short Neuropeptide F (sNPF) Family oF peptides and their cognate receptors play key roles in a variety oF physiological processes in arthropods. In silico screening oF GigasDatabase, a speciFic expressed sequence tag database From the PaciFic oyster Crassostrea gigas, resulted in the identiFication oF a receptor (Cg-sNPFR-like) phylogenetically closely related to sNPF receptors (sNPFRs) oF insects. A reverse endocrinology approach was undertaken to identiFy the peptide ligand(s) oF this orphan receptor. Though structurally distinct From insect sNPFs, three RFamide peptides derived From the same precursor, i.e. GSLFRFamide, SSLFRFamide and GALFRFamide, speciFically activate the receptor in a dose-dependent manner, with respective EC50 values (halF-maximal eFFective concentrations) oF 1.1, 2.1 and 4.1 mu mol l(-1). We Found that both Cg-sNPFR-like receptor and LFRFamide encoding transcripts are expressed in the oyster central nervous system and in other tissues as well, albeit at lower levels. Mass spectrometry analysis conFirmed the wide distribution oF LFRFamide mature peptides in several central and peripheral tissues. The Cg-sNPFR-like receptor was more abundantly expressed in ganglia oF Females than oF males, and upregulated in starved oysters. In the gonad area, highest receptor gene expression occurred at the start oF gametogenesis, when storage activity is maximal. Our results suggest that signaling oF LFRFamide peptides through the Cg-sNPFR-like receptor might play a role in the coordination oF nutrition, energy storage and metabolism in C. gigas, possibly by promoting storage at the expense oF reproduction.