The Experts below are selected from a list of 201 Experts worldwide ranked by ideXlab platform
Bernard Ollivier - One of the best experts on this subject based on the ideXlab platform.
-
Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, Nitrate-Reducing Bacterium isolated from an Australian oil well.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Monica Bonilla Salinas, Bharat K C Patel, Marie-laure Fardeau, Jean-luc Cayol, Laurence Casalot, Pierre Thomas, Jean-louis Garcia, Bernard OllivierAbstract:A novel Gram-negative, aerobic and moderately thermophilic Bacterium, strain 4BON(T), was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 degrees C, pH 6.9 and 0.5 % NaCl. Strain 4BON(T) was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or Nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58.6 mol%. The closest phylogenetic relative of strain 4BON(T) was Hydrogenophilus thermoluteolus (similarity of 91.8 %), of the beta-Proteobacteria. As strain 4BON(T) is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BON(T) (=DSM 15512(T)=CIP 107790(T)).
-
Reclassification of the sulfate- and Nitrate-Reducing Bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Alejandro López-cortés, Marie-laure Fardeau, Guy Fauque, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use Nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other Nitrate- and sulfate-Reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
-
Caldinitratiruptor microaerophilus, gen. nov., sp. nov. isolated from a French hot spring (Chaudes-Aigues, Massif Central): a novel cultivated facultative microaerophilic anaerobic thermophile pertaining to the SymbioBacterium branch within the Firmi
Extremophiles, 2010Co-Authors: Marie-laure Fardeau, Jean-luc Cayol, Vanessa Barsotti, Sophie Guasco, Valérie Michotey, Manon Joseph, Patricia Bonin, Bernard OllivierAbstract:A novel facultative microaerophilic Nitrate-Reducing Bacterium designated CA62N^T was isolated from a thermal spring in France. Cells were non-motile rods (2–3 × 0.2 μm) and showed low cytoplasmic density when observed under a phase-contrast microscope. Strain CA62N^T grew at temperatures between 50 and 75°C (optimum 65°C) and at a pH between 6.3 and 7.9 (optimum 7.0). NaCl was not required for growth but was tolerated up to 10 gl^−1. Sulfate, thiosulfate, elemental sulfur, sulfite, and nitrite were not used as electron acceptors. Nitrate was reduced to nitrite. Strain CA62N^T used lactate, pyruvate, glucose, mannose, fructose, and casamino acids and some amino acids as electron donors only in the presence of Nitrate as electron acceptor. None of these substrates was fermented. The main end-products of glucose oxidation were acetate, CO_2, and traces of H_2. The G + C content of the genomic DNA was 70.3 mol% (HPLC techniques). Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain CA62N^T was affiliated to the SymbioBacterium branch within the Firmicutes and had SymbioBacterium thermophilum and “ S. toebii ” as its closest phylogenetic relatives. On the basis of phylogenetical and physiological characteristics, strain CA62N^T is proposed to be the type strain for the novel species in the novel genus, Caldinitratiruptor microaerophilus gen. nov., sp. nov. (DSM 22660, JCM 16183).
-
reclassification of the sulfate and Nitrate Reducing Bacterium desulfovibrio vulgaris subsp oxamicus as desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Marie-laure Fardeau, Guy Fauque, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use Nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other Nitrate- and sulfate-Reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
-
petrobacter succinatimandens gen nov sp nov a moderately thermophilic Nitrate Reducing Bacterium isolated from an australian oil well
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Monica Bonilla Salinas, Bharat K C Patel, Marie-laure Fardeau, Jean-luc Cayol, Laurence Casalot, Pierre Thomas, Jean-louis Garcia, Bernard OllivierAbstract:A novel Gram-negative, aerobic and moderately thermophilic Bacterium, strain 4BONT, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BONT was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or Nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BONT was Hydrogenophilus thermoluteolus (similarity of 91·8 %), of the β-Proteobacteria. As strain 4BONT is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BONT (=DSM 15512T=CIP 107790T).
Marie-laure Fardeau - One of the best experts on this subject based on the ideXlab platform.
-
Petrobacter succinatimandens gen. nov., sp. nov., a moderately thermophilic, Nitrate-Reducing Bacterium isolated from an Australian oil well.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Monica Bonilla Salinas, Bharat K C Patel, Marie-laure Fardeau, Jean-luc Cayol, Laurence Casalot, Pierre Thomas, Jean-louis Garcia, Bernard OllivierAbstract:A novel Gram-negative, aerobic and moderately thermophilic Bacterium, strain 4BON(T), was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 degrees C, pH 6.9 and 0.5 % NaCl. Strain 4BON(T) was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or Nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58.6 mol%. The closest phylogenetic relative of strain 4BON(T) was Hydrogenophilus thermoluteolus (similarity of 91.8 %), of the beta-Proteobacteria. As strain 4BON(T) is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BON(T) (=DSM 15512(T)=CIP 107790(T)).
-
Reclassification of the sulfate- and Nitrate-Reducing Bacterium Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Alejandro López-cortés, Marie-laure Fardeau, Guy Fauque, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925(T)) was found to use Nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925(T) was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other Nitrate- and sulfate-Reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925(T). This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 degrees C (range 16-50 degrees C) and the optimum NaCl concentration for growth was 0.1 % (range 0-5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2(T) (=DSM 1925(T)=NCIMB 9442(T)=ATCC 33405(T)).
-
Caldinitratiruptor microaerophilus, gen. nov., sp. nov. isolated from a French hot spring (Chaudes-Aigues, Massif Central): a novel cultivated facultative microaerophilic anaerobic thermophile pertaining to the SymbioBacterium branch within the Firmi
Extremophiles, 2010Co-Authors: Marie-laure Fardeau, Jean-luc Cayol, Vanessa Barsotti, Sophie Guasco, Valérie Michotey, Manon Joseph, Patricia Bonin, Bernard OllivierAbstract:A novel facultative microaerophilic Nitrate-Reducing Bacterium designated CA62N^T was isolated from a thermal spring in France. Cells were non-motile rods (2–3 × 0.2 μm) and showed low cytoplasmic density when observed under a phase-contrast microscope. Strain CA62N^T grew at temperatures between 50 and 75°C (optimum 65°C) and at a pH between 6.3 and 7.9 (optimum 7.0). NaCl was not required for growth but was tolerated up to 10 gl^−1. Sulfate, thiosulfate, elemental sulfur, sulfite, and nitrite were not used as electron acceptors. Nitrate was reduced to nitrite. Strain CA62N^T used lactate, pyruvate, glucose, mannose, fructose, and casamino acids and some amino acids as electron donors only in the presence of Nitrate as electron acceptor. None of these substrates was fermented. The main end-products of glucose oxidation were acetate, CO_2, and traces of H_2. The G + C content of the genomic DNA was 70.3 mol% (HPLC techniques). Phylogenetic analysis of the small-subunit (SSU) ribosomal RNA (rRNA) gene sequence indicated that strain CA62N^T was affiliated to the SymbioBacterium branch within the Firmicutes and had SymbioBacterium thermophilum and “ S. toebii ” as its closest phylogenetic relatives. On the basis of phylogenetical and physiological characteristics, strain CA62N^T is proposed to be the type strain for the novel species in the novel genus, Caldinitratiruptor microaerophilus gen. nov., sp. nov. (DSM 22660, JCM 16183).
-
reclassification of the sulfate and Nitrate Reducing Bacterium desulfovibrio vulgaris subsp oxamicus as desulfovibrio oxamicus sp nov comb nov
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Alejandro Lopezcortes, Marie-laure Fardeau, Guy Fauque, Catherine Joulian, Bernard OllivierAbstract:Desulfovibrio vulgaris subsp. oxamicus (type strain, DSM 1925T) was found to use Nitrate as a terminal electron acceptor, the latter being reduced to ammonium. Phylogenetic studies indicated that strain DSM 1925T was distantly related to the type strain of Desulfovibrio vulgaris (95.4 % similarity of the small-subunit rRNA gene) and had as its closest phylogenetic relatives two other Nitrate- and sulfate-Reducing bacteria, namely Desulfovibrio termitidis (99.4 % similarity) and Desulfovibrio longreachensis (98.4 % similarity). Additional experiments were conducted to characterize better strain DSM 1925T. This strain incompletely oxidized lactate and ethanol to acetate. It also oxidized butanol, pyruvate and citrate, but not glucose, fructose, acetate, propionate, butyrate, methanol, glycerol or peptone. The optimum temperature for growth was 37 °C (range 16–50 °C) and the optimum NaCl concentration for growth was 0.1 % (range 0–5 %). Because of significant genotypic and phenotypic differences from Desulfovibrio termitidis and Desulfovibrio longreachensis, reclassification of Desulfovibrio vulgaris subsp. oxamicus as Desulfovibrio oxamicus sp. nov., comb. nov., is proposed. The type strain is strain Monticello 2T (=DSM 1925T=NCIMB 9442T=ATCC 33405T).
-
petrobacter succinatimandens gen nov sp nov a moderately thermophilic Nitrate Reducing Bacterium isolated from an australian oil well
International Journal of Systematic and Evolutionary Microbiology, 2004Co-Authors: Monica Bonilla Salinas, Bharat K C Patel, Marie-laure Fardeau, Jean-luc Cayol, Laurence Casalot, Pierre Thomas, Jean-louis Garcia, Bernard OllivierAbstract:A novel Gram-negative, aerobic and moderately thermophilic Bacterium, strain 4BONT, was isolated from a non-water-flooded Australian terrestrial oil reservoir. Cells were non-spore-forming straight rods, which were motile by means of a polar flagellum. The optimum growth conditions were 55 °C, pH 6·9 and 0·5 % NaCl. Strain 4BONT was oxidase- and catalase-positive; it grew on fumarate, pyruvate, succinate, formate, ethanol and yeast extract in the presence of oxygen or Nitrate as terminal electron acceptor. Nitrate was reduced to nitrous oxide. The DNA G+C content of the strain was 58·6 mol%. The closest phylogenetic relative of strain 4BONT was Hydrogenophilus thermoluteolus (similarity of 91·8 %), of the β-Proteobacteria. As strain 4BONT is physiologically and phylogenetically different from H. thermoluteolus, it is proposed that it be assigned to a novel species of a novel genus, Petrobacter succinatimandens gen. nov., sp. nov. The type strain is 4BONT (=DSM 15512T=CIP 107790T).
Erko Stackebrandt - One of the best experts on this subject based on the ideXlab platform.
-
vulcanibacillus modesticaldus gen nov sp nov a strictly anaerobic Nitrate Reducing Bacterium from deep sea hydrothermal vents
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Stephane Lharidon, Erko Stackebrandt, M L Miroshnichenko, N A Kostrikina, Brian J Tindall, Stefan Spring, Peter Schumann, Elizaveta A Bonchosmolovskaya, Christian JeanthonAbstract:A novel anaerobic, moderately thermophilic, spore-forming Bacterium, designated strain BR T , was isolated from deep-sea hydrothermal core samples collected at the Rainbow vent field on the Mid-Atlantic Ridge (366 149 N3 36 549 W). The cells were found to be rod-shaped, non-motile, Gram-positive and spore-forming. The organism grew in the temperature range 37‐60 6C, with an optimum at 55 6C, and at pH values in the range 6‐8?5, with an optimum around pH 7. NaCl concentrations for growth were in the range 10‐40 g l ”1 , with an optimum at 20‐30 g l ”1 . Strain BR T grew chemo-organoheterotrophically with carbohydrates, proteinaceous substrates and organic acidswith Nitrate as electron acceptor. Thenovel isolatewasnot ableto ferment. TheG+C content of the genomic DNA was 34?5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain BR T in the Bacillaceae within the class ‘Bacilli’. On the basis of the phenotypic and phylogenetic data, this isolate should be described as a member of a novel genus, for which the name Vulcanibacillus gen. nov. is proposed. The type species is Vulcanibacillus modesticaldus sp. nov., with the type strain BR T (=DSM 14931 T =JCM 12998 T ).
-
gordonia sihwensis sp nov a novel Nitrate Reducing Bacterium isolated from a wastewater treatment bioreactor
International Journal of Systematic and Evolutionary Microbiology, 2003Co-Authors: Reiner M Kroppenstedt, Erko StackebrandtAbstract:A Nitrate-Reducing Bacterium, strain SPR2T, was isolated from a sulphur-oxidizing, autotrophic denitrification reactor used for advanced treatment of wastewater from the lake of Sihwa, Korea. The strain was aerobic but could grow under anaerobic conditions. It was Gram-positive, exhibited rough white colonies on complex nutrient agar, produced elementary branching hyphae that fragmented into rod/coccus-like elements and showed chemotaxonomic markers that were consistent with classification in the genus Gordonia, i.e. meso-diaminopimelic acid, arabinose and galactose in whole-cell hydrolysates, N-glycolylmuramic acid in the peptidoglycan wall, unbranched saturated and monounsaturated fatty acids plus tuberculostearic acid (TBSA), mycolic acids that comprised 48–56 carbon atoms and MK-9(H2) as the predominant menaquinone. The 16S rDNA sequence of strain SPR2T showed highest similarity to Gordonia amicalis DSM 44461T and Gordonia hydrophobica DSM 44015T, with values of 98·2 and 97·9 %, respectively. These values were far below 99·5 % (usually found at the intraspecies level) and they were in the range that separates species at the intrageneric level. The separate phylogenetic position of SPR2T was supported by differences in fatty acid and mycolic acid compositions and in carbon utilization tests that distinguished strain SPR2T from all known Gordonia species. Combined genotypic and phenotypic data show that strain SPR2T merits recognition as a novel species within the genus Gordonia, for which the name Gordonia sihwensis sp. nov. is proposed; the type strain is SPR2T (=DSM 44576T=NRRL B-24155T).
-
isolation and characterization of a new gram negative acetone degrading Nitrate Reducing Bacterium from soil paracoccus solventivorans sp nov
International Journal of Systematic and Evolutionary Microbiology, 1996Co-Authors: Hubert Siller, Erko Stackebrandt, Fred A Rainey, J WinterAbstract:An acetone-degrading, Nitrate-Reducing, coccoid to rod-shaped Bacterium, strain L1, was isolated from soil on the site of a natural gas company. Cells of the logarithmic growth phase reacted gram positive, while those of the stationary growth phase were gram negative. Single organisms were 0.4 to 0.5 by 0.9 to 1.5 μm in size, nonmotile, and non-spore forming and had poly-β-hydroxybutyrate inclusions. The doubling time of strain L1 on acetone-CO2-Nitrate at the optimal pH of 7 to 8 and the optimal temperature of 30 to 37°C was 12 h. More than 0.2% NaCl or 10 mM thiosulfate inhibited growth. For oxygen or Nitrate respiration, acetone and a few organic chemicals were utilized as carbon sources whereas many others could not be used (for details, see Results). Bicarbonate (or CO2) was essential for growth on acetone but not for growth on acetoacetate. The growth yields for acetone-CO2 and acetoacetate were 28.3 and 27.3 g/mol, respectively. With acetone as the carbon source, poly-β-hydroxybutyrate accounted for up to 40% of the cellular dry weight The DNA of strain L1 had a G+C content of 68.5 mol% (as determined by high-performance liquid chromatography of nucleotides) or 70 mol% (as determined by the Tm method). The sequence of the gene coding for the 16S rRNA led to the classification of strain L1 in the paracoccus group of the alpha subclass of the Proteobacteria. The new isolate is named Paracoccus solventivorans sp. nov. DSM 6637.
Joonhong Park - One of the best experts on this subject based on the ideXlab platform.
-
nocardioides daeguensis sp nov a Nitrate Reducing Bacterium isolated from activated sludge of an industrial wastewater treatment plant
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Sahastranshu Sinha, Myung Suk Kang, Joonhong ParkAbstract:A Gram-reaction-positive, rod-shaped, non-spore-forming Bacterium (strain 2C1-5T) was isolated from activated sludge of an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, the closest phylogenetic relatives were the type strains of Nocardioides nitrophenolicus (98.6 % similarity), N. kongjuensis (98.5 %), N. caeni (98.4 %), N. simplex (98.3 %), N. aromaticivorans (98.1 %) and N. ginsengisoli (97.5 %); the phylogenetic distance from other species with validly published names within the genus Nocardioides was greater than 3 %. Strain 2C1-5T was characterized chemotaxonomically as having ll-2,6-diaminopimelic acid in the cell-wall peptidoglycan, MK-8(H4) as the predominant menaquinone and iso-C16 : 0, C16 : 0 and C17 : 1ω6c as the major fatty acids. The G+C content of the genomic DNA was 74.9 mol%. These chemotaxonomic properties and phenotypic characteristics supported the affiliation of strain 2C1-5T to the genus Nocardioides . The results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain 2C1-5T from existing species with validly published names. Therefore, strain 2C1-5T represents a novel species of the genus Nocardioides , for which the name Nocardioides daeguensis sp. nov. is proposed, with the type strain 2C1-5T ( = JCM 17460T = KCTC 19799T).
-
breviBacterium daeguense sp nov a Nitrate Reducing Bacterium isolated from a 4 chlorophenol enrichment culture
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Myung Suk Kang, Joonhong ParkAbstract:A Gram-reaction-positive, non-spore-forming, aerobic actinobacterial strain (2C6-41T) was isolated from the activated sludge from an industrial wastewater treatment plant in Daegu, South Korea. Its taxonomic position was investigated by using a polyphasic approach. On the basis of 16S rRNA gene sequence similarity, closest phylogenetic relatives to strain 2C6-41T were BreviBacterium pityocampae DSM 21720T (97.2 %), BreviBacterium salitolerans KCTC 19616T (96.7 %), BreviBacterium album KCTC 19173T (96.2 %) and BreviBacterium samyangense KCCM 42316T (96.2 %). The DNA G+C content of strain 2C6-41T was 66.4 mol%. Chemotaxonomic data, which included MK-8(H2) as the major menaquinone; meso-diaminopimelic acid, glutamic acid and alanine as cell-wall amino acids; ribose, mannose and glucose as major cell-wall sugars; and anteiso-C15 : 0, anteiso-C17 : 0, C16 : 0 and iso-C15 : 0 as major fatty acids, supported the affiliation of strain 2C6-41T to the genus BreviBacterium . The aromatic ring cleavage enzyme catechol 1,2-dioxygenase was not detected in strain 2C6-41T, but catechol 2,3-dioxygenase was detected. The results of physiological and biochemical tests, and the low level of DNA–DNA relatedness to the closest phylogenetic relative enabled strain 2C6-41T to be differentiated genotypically and phenotypically from recognized species of the genus BreviBacterium . The isolate is therefore considered to represent a novel species in the genus BreviBacterium , for which the name BreviBacterium daeguense sp. nov. is proposed. The type strain is 2C6-41T ( = KCTC 19800T = JCM 17458T).
-
ochrobactrum daejeonense sp nov a Nitrate Reducing Bacterium isolated from sludge of a leachate treatment plant
International Journal of Systematic and Evolutionary Microbiology, 2011Co-Authors: Joonhong ParkAbstract:A Gram-reaction-negative, non-spore-forming, rod-shaped, aerobic bacterial strain, designated MJ11(T), was isolated from sludge of a leachate treatment plant in Daejeon, South Korea, and was characterized taxonomically by using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain MJ11(T) belonged to the family Brucellaceae, class Alphaproteobacteria, and was most closely related to Ochrobactrum ciceri Ca-34(T) (97.9 % sequence similarity) and Ochrobactrum pituitosum CCUG 50899(T) (96.4 %). Comparative sequence analyses of the additional phylogenetic marker genes dnaK, groEL and gyrB confirmed the affiliation of strain MJ11(T) to the genus Ochrobactrum. The G+C content of the genomic DNA of strain MJ11(T) was 59.3 mol%. The detection of a quinone system with ubiquinone Q-10 as the predominant respiratory lipoquinone, a fatty acid profile with C(18 : 1)ω7c (62.6 %) and C(19 : 0) cyclo ω8c (14.2 %) as the major components, a polar lipid profile with phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylmonomethylethanolamine, diphosphatidylglycerol and unknown aminolipids AL1 and AL2 as major polar lipids and spermidine and putrescine as the predominant polyamines also supported the affiliation of strain MJ11(T) to the genus Ochrobactrum. The DNA-DNA relatedness between strain MJ11(T) and Ochrobactrum ciceri DSM 22292(T) was 29 ± 7 %, clearly showing that the isolate constitutes a new genospecies. Strain MJ11(T) could be clearly differentiated from its closest neighbours on the basis of its phenotypic, genotypic and chemotaxonomic features. Therefore, strain MJ11(T) represents a novel species of the genus Ochrobactrum, for which the name Ochrobactrum daejeonense sp. nov. is proposed. The type strain is MJ11(T) ( = KCTC 22458(T) = JCM 16234(T)).
Christian Jeanthon - One of the best experts on this subject based on the ideXlab platform.
-
Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, Nitrate-Reducing Bacterium from deep-sea hydrothermal vents.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: S L'haridon, M L Miroshnichenko, N A Kostrikina, Brian J Tindall, Stefan Spring, Peter Schumann, E Stackebrandt, E A Bonch-osmolovskaya, Christian JeanthonAbstract:A novel anaerobic, moderately thermophilic, spore-forming Bacterium, designated strain BRT, was isolated from deep-sea hydrothermal core samples collected at the Rainbow vent field on the Mid-Atlantic Ridge (36 degrees 14' N 33 degrees 54' W). The cells were found to be rod-shaped, non-motile, Gram-positive and spore-forming. The organism grew in the temperature range 37-60 degrees C, with an optimum at 55 degrees C, and at pH values in the range 6-8.5, with an optimum around pH 7. NaCl concentrations for growth were in the range 10-40 g l(-1), with an optimum at 20-30 g l(-1). Strain BRT grew chemo-organoheterotrophically with carbohydrates, proteinaceous substrates and organic acids with Nitrate as electron acceptor. The novel isolate was not able to ferment. The G+C content of the genomic DNA was 34.5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain BRT in the Bacillaceae within the class 'Bacilli'. On the basis of the phenotypic and phylogenetic data, this isolate should be described as a member of a novel genus, for which the name Vulcanibacillus gen. nov. is proposed. The type species is Vulcanibacillus modesticaldus sp. nov., with the type strain BRT (=DSM 14931T=JCM 12998T).
-
vulcanibacillus modesticaldus gen nov sp nov a strictly anaerobic Nitrate Reducing Bacterium from deep sea hydrothermal vents
International Journal of Systematic and Evolutionary Microbiology, 2006Co-Authors: Stephane Lharidon, Erko Stackebrandt, M L Miroshnichenko, N A Kostrikina, Brian J Tindall, Stefan Spring, Peter Schumann, Elizaveta A Bonchosmolovskaya, Christian JeanthonAbstract:A novel anaerobic, moderately thermophilic, spore-forming Bacterium, designated strain BR T , was isolated from deep-sea hydrothermal core samples collected at the Rainbow vent field on the Mid-Atlantic Ridge (366 149 N3 36 549 W). The cells were found to be rod-shaped, non-motile, Gram-positive and spore-forming. The organism grew in the temperature range 37‐60 6C, with an optimum at 55 6C, and at pH values in the range 6‐8?5, with an optimum around pH 7. NaCl concentrations for growth were in the range 10‐40 g l ”1 , with an optimum at 20‐30 g l ”1 . Strain BR T grew chemo-organoheterotrophically with carbohydrates, proteinaceous substrates and organic acidswith Nitrate as electron acceptor. Thenovel isolatewasnot ableto ferment. TheG+C content of the genomic DNA was 34?5 mol%. Phylogenetic analysis of the 16S rRNA gene sequence placed strain BR T in the Bacillaceae within the class ‘Bacilli’. On the basis of the phenotypic and phylogenetic data, this isolate should be described as a member of a novel genus, for which the name Vulcanibacillus gen. nov. is proposed. The type species is Vulcanibacillus modesticaldus sp. nov., with the type strain BR T (=DSM 14931 T =JCM 12998 T ).