The Experts below are selected from a list of 99 Experts worldwide ranked by ideXlab platform
Katarzyna Slowinska - One of the best experts on this subject based on the ideXlab platform.
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
Alicia Gamboa - One of the best experts on this subject based on the ideXlab platform.
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
Vitaly Citovsky - One of the best experts on this subject based on the ideXlab platform.
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Nucleic Acid Transport in plant microbe interactions the molecules that walk through the walls
Annual Review of Microbiology, 2000Co-Authors: Tzvi Tzfira, Yoon Rhee, Minhuei Chen, Talya Kunik, Vitaly CitovskyAbstract:▪ Abstract Many microbes “genetically invade” plants by introducing DNA or RNA molecules into the host cells. For example, plant viruses Transport their genomes between host cells, whereas Agrobacterium spp. transfer T-DNA to the cell nucleus and integrate it into the plant DNA. During these events, the Transported Nucleic Acids must negotiate several barriers, such as plant cell walls, plasma membranes, and nuclear envelopes. This review describes the microbial and host proteins that participate in cell-to-cell Transport and nuclear import of Nucleic Acids during infection by plant viruses and Agrobacterium spp. Possible molecular mechanisms by which these Transport processes occur are discussed.
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Nucleic Acid Transport in plant pathogen interactions
Genetic engineering, 1997Co-Authors: Robert T Lartey, Vitaly CitovskyAbstract:Transport of Nucleic Acid molecules is central to many plant-pathogen interactions. Nucleic Acids are Transported between cells when plant viruses move their genomes from the infected into adjacent uninfected cells through plant intercellular connections, the plasmodesmata. DNA and RNA molecules are also Transported from the host cell cytoplasm into the nucleus during many viral infections. In addition, nuclear import of DNA is a central event in genetic transformation of plant cells by a soil microorganism Agrobacterium tumefaciens. Recent studies suggest that both intercellular Transport and nuclear import of Nucleic Acids are mediated by specific pathogen-encoded proteins. These proteins are thought to form complexes with the Transported Nucleic Acid molecule and supply it with the targeting signals which specify the Transport process. In this review, we focus on the cell-to-cell movement proteins of plant viruses and the DNA nuclear import proteins of Agrobacterium.
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Transport of protein—Nucleic Acid complexes within and between plant cells
Membrane Protein Transport, 1995Co-Authors: Vitaly Citovsky, Patricia ZambryskiAbstract:Publisher Summary In eukaryotic organisms, Nucleic Acid molecules are constantly Transported between the nucleus and the cytoplasm. This Transport includes nuclear export and import of RNAs and nuclear import of retrotransposable elements and invading viruses. Higher plants have an additional Nucleic Acid Transport system that allows infectious viral genomes to be Transported between adjacent host cells. Nuclear import and plasmodesmal Transport of Nucleic Acids in plants exhibit several structural and functional similarities. This chapter focuses on two processes of Nucleic Acid Transport in plants: (1) the Transport of Agrobacterium single-stranded (ss) DNA through nuclear pores and (2) the movement of tobacco mosaic virus (TMV) genomic RNA through plant intercellular connections, the plasmodesmata. Both processes involve single-stranded Nucleic Acids that are Transported as complexes with specialized Transport proteins. These proteins bind ssDNA and RNA, producing thin unfolded filaments of protein–Nucleic Acid Transport complexes. In addition to shaping Nucleic Acid molecules in a transferable form, the Transport proteins also provide specific signals for targeting and interaction with the nuclear pores or plasmodesmal channels.
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Transport of protein Nucleic Acid complexes within and between plant cells
Membrane Protein Transport, 1995Co-Authors: Vitaly Citovsky, Patricia ZambryskiAbstract:Publisher Summary In eukaryotic organisms, Nucleic Acid molecules are constantly Transported between the nucleus and the cytoplasm. This Transport includes nuclear export and import of RNAs and nuclear import of retrotransposable elements and invading viruses. Higher plants have an additional Nucleic Acid Transport system that allows infectious viral genomes to be Transported between adjacent host cells. Nuclear import and plasmodesmal Transport of Nucleic Acids in plants exhibit several structural and functional similarities. This chapter focuses on two processes of Nucleic Acid Transport in plants: (1) the Transport of Agrobacterium single-stranded (ss) DNA through nuclear pores and (2) the movement of tobacco mosaic virus (TMV) genomic RNA through plant intercellular connections, the plasmodesmata. Both processes involve single-stranded Nucleic Acids that are Transported as complexes with specialized Transport proteins. These proteins bind ssDNA and RNA, producing thin unfolded filaments of protein–Nucleic Acid Transport complexes. In addition to shaping Nucleic Acid molecules in a transferable form, the Transport proteins also provide specific signals for targeting and interaction with the nuclear pores or plasmodesmal channels.
Karin B Michels - One of the best experts on this subject based on the ideXlab platform.
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The causal effect of red blood cell folate on genome-wide methylation in cord blood: a Mendelian randomization approach
BMC Bioinformatics, 2013Co-Authors: Alexandra M Binder, Karin B MichelsAbstract:Background Investigation of the biological mechanism by which folate acts to affect fetal development can inform appraisal of expected benefits and risk management. This research is ethically imperative given the ubiquity of folic Acid fortified products in the US. Considering that folate is an essential component in the one-carbon metabolism pathway that provides methyl groups for DNA methylation, epigenetic modifications provide a putative molecular mechanism mediating the effect of folic Acid supplementation on neonatal and pediatric outcomes. Results In this study we use a Mendelian Randomization Unnecessary approach to assess the effect of red blood cell (RBC) folate on genome-wide DNA methylation in cord blood. Site-specific CpG methylation within the proximal promoter regions of approximately 14,500 genes was analyzed using the Illumina Infinium Human Methylation27 Bead Chip for 50 infants from the Epigenetic Birth Cohort at Brigham and Women’s Hospital in Boston. Using methylenetetrahydrofolate reductase genotype as the instrument, the Mendelian Randomization approach identified 7 CpG loci with a significant (mostly positive) association between RBC folate and methylation level. Among the genes in closest proximity to this significant subset of CpG loci, several enriched biologic processes were involved in Nucleic Acid Transport and metabolic processing. Compared to the standard ordinary least squares regression method, our estimates were demonstrated to be more robust to unmeasured confounding. Conclusions To the authors’ knowledge, this is the largest genome-wide analysis of the effects of folate on methylation pattern, and the first to employ Mendelian Randomization to assess the effects of an exposure on epigenetic modifications. These results can help guide future analyses of the causal effects of periconceptional folate levels on candidate pathways.
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The causal effect of red blood cell folate on genome-wide methylation in cord blood: a Mendelian randomization approach
BMC Bioinformatics, 2013Co-Authors: Alexandra M Binder, Karin B MichelsAbstract:Investigation of the biological mechanism by which folate acts to affect fetal development can inform appraisal of expected benefits and risk management. This research is ethically imperative given the ubiquity of folic Acid fortified products in the US. Considering that folate is an essential component in the one-carbon metabolism pathway that provides methyl groups for DNA methylation, epigenetic modifications provide a putative molecular mechanism mediating the effect of folic Acid supplementation on neonatal and pediatric outcomes. In this study we use a Mendelian Randomization Unnecessary approach to assess the effect of red blood cell (RBC) folate on genome-wide DNA methylation in cord blood. Site-specific CpG methylation within the proximal promoter regions of approximately 14,500 genes was analyzed using the Illumina Infinium Human Methylation27 Bead Chip for 50 infants from the Epigenetic Birth Cohort at Brigham and Women’s Hospital in Boston. Using methylenetetrahydrofolate reductase genotype as the instrument, the Mendelian Randomization approach identified 7 CpG loci with a significant (mostly positive) association between RBC folate and methylation level. Among the genes in closest proximity to this significant subset of CpG loci, several enriched biologic processes were involved in Nucleic Acid Transport and metabolic processing. Compared to the standard ordinary least squares regression method, our estimates were demonstrated to be more robust to unmeasured confounding. To the authors’ knowledge, this is the largest genome-wide analysis of the effects of folate on methylation pattern, and the first to employ Mendelian Randomization to assess the effects of an exposure on epigenetic modifications. These results can help guide future analyses of the causal effects of periconceptional folate levels on candidate pathways.
Katrina Hernandez - One of the best experts on this subject based on the ideXlab platform.
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
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Higher Order Architecture of Designer Peptides Forms Bioinspired 10 nm siRNA Delivery System
Scientific Reports, 2019Co-Authors: Alicia Gamboa, Selina F. Urfano, Katrina Hernandez, Deborah A. Fraser, Luladey Ayalew, Katarzyna SlowinskaAbstract:The higher-order architecture observed in biological systems, like viruses, is very effective in Nucleic Acid Transport. The replications of this system has been attempted with both synthetic and naturally occurring polymers with mixed results. Here we describe a peptide/siRNA quaternary complex that functions as an siRNA delivery system. The rational design of a peptide assembly is inspired by the viral capsids, but not derived from them. We selected the collagen peptide (COL) to provide the structural stability and the folding framework, and hybridize it with the cell penetrating peptide (CPP) that allows for effective penetration of biological barriers. The peptide/siRNA quaternary complex forms stoichiometric, 10 nm nanoparticles, that show fast cellular uptake (
