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Carlos Simón - One of the best experts on this subject based on the ideXlab platform.
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endometrial thickness and serum Oestradiol concentrations as predictors of outcome in oocyte donation
Human Reproduction, 1997Co-Authors: J Remohi, Carlos Simón, Gerardo Ardiles, J A Garciavelasco, P Gaitan, A PellicerAbstract:Adequate endometrial preparation with exogenous steroidsis mandatory for successful ovum donation. This study wasundertaken to assess the value of endometrial thickness byultrasound and serum Oestradiol as predictors of ovumdonation outcome and to analyse the correlation betweenserum Oestradiol concentrations and the endometrial thick-ness. Endometrial thickness and serum Oestradiol concen-trations on the day of oocyte donation were recorded andcompared to several in-vitro fertilization parameters. Thecycles (n J 465) were classified according to serum oest-radiol values and endometrial thickness. Comparison ofthe groups showed that endometrial thickness was signi-ficantly (P J 0.002) higher when serum Oestradiol was
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endometrial thickness and serum Oestradiol concentrations as predictors of outcome in oocyte donation
Human Reproduction, 1997Co-Authors: J Remohi, Carlos Simón, Gerardo Ardiles, J A Garciavelasco, P Gaitan, A PellicerAbstract:Adequate endometrial preparation with exogenous steroids is mandatory for successful ovum donation. This study was undertaken to assess the value of endometrial thickness by ultrasound and serum Oestradiol as predictors of ovum donation outcome and to analyse the correlation between serum Oestradiol concentrations and the endometrial thickness. Endometrial thickness and serum Oestradiol concentrations on the day of oocyte donation were recorded and compared to several in-vitro fertilization parameters. The cycles (n = 465) were classified according to serum Oestradiol values and endometrial thickness. Comparison of the groups showed that endometrial thickness was significantly (P = 0.002) higher when serum Oestradiol was >400 pg/ml as compared to <100 pg/ml. Pregnancy and implantation rates did not differ among the groups, women with serum Oestradiol <50 pg/ml having similar outcome to the remaining cases. Endometrial thickness showed a similar picture in terms of pregnancy and implantation. Also, women with an endometrium <4 mm in size had normal pregnancy and implantation rates. There was a positive correlation (P = 0.0044) between endometrial thickness and implantation, as well as between endometrial thickness and serum Oestradiol (P = 0.0184). None of the parameters examined was able to predict ovum donation outcome. It is concluded that endometrial thickness is preferred to serum Oestradiol for the monitoring of endometrial development, although neither is able to predict success in oocyte donation.
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clinical evidence for a detrimental effect on uterine receptivity of high serum Oestradiol concentrations in high and normal responder patients
Human Reproduction, 1995Co-Authors: Carlos Simón, Fidel Cano, Diana Valbuena, Antonio PellicerAbstract:This study was undertaken to investigate an empirical observation that 'high responder patients have poorer in-vitro fertilization (IVF) outcome than normal responder patients'. The aim of our study was to analyse the effect of high serum Oestradiol and progesterone concentrations at the day of human chorionic gonadotrophin (HCG) administration on endometrial receptivity and oocyte-embryo quality in high and normal responder patients. The IVF patients were divided into two groups : 59 high responder patients who voluntarily donated some of their oocytes, and a control group consisting of 105 normal responder patients. Both groups were compared in terms of the number and quality of oocytes retrieved, embryos transferred, fertilization, implantation and gestation rates, serum Oestradiol and progesterone concentrations and the Oestradiol :progesterone ratio on the day of HCG injection. To ascertain oocyte-embryo quality, a second control group of 96 women undergoing oocyte donation (receiving oocytes from high responder patients) was considered. To assess the impact of steroid concentrations on endometrial receptivity, high responder patients were divided into two subgroups according to Oestradiol concentration, above or below the minimal Oestradiol and progesterone concentrations (mean - SD) in this group. The normal responder patients were divided into two subgroups according to Oestradiol concentration, above or below the maximal Oestradiol and progesterone concentrations (mean + SD) in this group. To assess further the relevance of Oestradiol concentration on endometrial receptivity, patients were divided into different subgroups according to increasing Oestradiol concentration, regardless of whether they were high or normal responders. High responder patients had significantly decreased implantation and pregnancy rates per cycle compared with normal responder patients (33.3 versus 16.3 and 11.1 versus 5.4% respectively ; P 1700 pg/ml compared with those having Oestradiol concentrations ≤1700 pg/ml, as well as in normal responder patients with serum Oestradiol concentrations >2200 pg/ml compared with those having Oestradiol concentrations ≤2200 pg/ml. Considering all the patients together, significant decreases in pregnancy and implantation rates were observed when Oestradiol concentrations were >2500 pg/ml compared with patients having lower Oestradiol concentrations. Our clinical results demonstrate that high serum Oestradiol concentrations on the day of HCG injection in high and normal responder patients, regardless of the number of oocytes retrieved and the serum progesterone concentration, are detrimental to uterine receptivity without affecting embryo quality.
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clinical evidence for a detrimental effect on uterine receptivity of high serum Oestradiol concentrations in high and normal responder patients
Human Reproduction, 1995Co-Authors: Carlos Simón, Fidel Cano, Diana Valbuena, J Remohi, Antonio PellicerAbstract:This study was undertaken to investigate an empirical observation that 'high responder patients have poorer in-vitro fertilization (IVF) outcome than normal responder patients'. The aim of our study was to analyse the effect of high serum Oestradiol and progesterone concentrations at the day of human chorionic gonadotrophin (HCG) administration on endometrial receptivity and oocyte-embryo quality in high and normal responder patients. The IVF patients were divided into two groups: 59 high responder patients who voluntarily donated some of their oocytes, and a control group consisting of 105 normal responder patients. Both groups were compared in terms of the number and quality of oocytes retrieved, embryos transferred, fertilization, implantation and gestation rates, serum Oestradiol and progesterone concentrations and the Oestradiol:progesterone ratio on the day of HCG injection. To ascertain oocyte-embryo quality, a second control group of 96 women undergoing oocyte donation (receiving oocytes from high responder patients) was considered. To assess the impact of steroid concentrations on endometrial receptivity, high responder patients were divided into two subgroups according to Oestradiol concentration, above or below the minimal Oestradiol and progesterone concentrations (mean--SD) in this group. The normal responder patients were divided into two subgroups according to Oestradiol concentration, above or below the maximal Oestradiol and progesterone concentrations (mean+SD) in this group. To assess further the relevance of Oestradiol concentration on endometrial receptivity, patients were divided into different subgroups according to increasing Oestradiol concentration, regardless of whether they were high or normal responders. High responder patients had significantly decreased implantation and pregnancy rates per cycle compared with normal responder patients (33.3 versus 16.3 and 11.1 versus 5.4% respectively; P 1700 pg/ml compared with those having Oestradiol concentrations 2200 pg/ml compared with those having Oestradiol concentrations 2500 pg/ml compared with patients having lower Oestradiol concentrations. Our clinical results demonstrate that high serum Oestradiol concentrations on the day of HCG injection in high and normal responder patients, regardless of the number of oocytes retrieved and the serum progesterone concentration, are detrimental to uterine receptivity without affecting embryo quality.
Antonio Pellicer - One of the best experts on this subject based on the ideXlab platform.
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clinical evidence for a detrimental effect on uterine receptivity of high serum Oestradiol concentrations in high and normal responder patients
Human Reproduction, 1995Co-Authors: Carlos Simón, Fidel Cano, Diana Valbuena, Antonio PellicerAbstract:This study was undertaken to investigate an empirical observation that 'high responder patients have poorer in-vitro fertilization (IVF) outcome than normal responder patients'. The aim of our study was to analyse the effect of high serum Oestradiol and progesterone concentrations at the day of human chorionic gonadotrophin (HCG) administration on endometrial receptivity and oocyte-embryo quality in high and normal responder patients. The IVF patients were divided into two groups : 59 high responder patients who voluntarily donated some of their oocytes, and a control group consisting of 105 normal responder patients. Both groups were compared in terms of the number and quality of oocytes retrieved, embryos transferred, fertilization, implantation and gestation rates, serum Oestradiol and progesterone concentrations and the Oestradiol :progesterone ratio on the day of HCG injection. To ascertain oocyte-embryo quality, a second control group of 96 women undergoing oocyte donation (receiving oocytes from high responder patients) was considered. To assess the impact of steroid concentrations on endometrial receptivity, high responder patients were divided into two subgroups according to Oestradiol concentration, above or below the minimal Oestradiol and progesterone concentrations (mean - SD) in this group. The normal responder patients were divided into two subgroups according to Oestradiol concentration, above or below the maximal Oestradiol and progesterone concentrations (mean + SD) in this group. To assess further the relevance of Oestradiol concentration on endometrial receptivity, patients were divided into different subgroups according to increasing Oestradiol concentration, regardless of whether they were high or normal responders. High responder patients had significantly decreased implantation and pregnancy rates per cycle compared with normal responder patients (33.3 versus 16.3 and 11.1 versus 5.4% respectively ; P 1700 pg/ml compared with those having Oestradiol concentrations ≤1700 pg/ml, as well as in normal responder patients with serum Oestradiol concentrations >2200 pg/ml compared with those having Oestradiol concentrations ≤2200 pg/ml. Considering all the patients together, significant decreases in pregnancy and implantation rates were observed when Oestradiol concentrations were >2500 pg/ml compared with patients having lower Oestradiol concentrations. Our clinical results demonstrate that high serum Oestradiol concentrations on the day of HCG injection in high and normal responder patients, regardless of the number of oocytes retrieved and the serum progesterone concentration, are detrimental to uterine receptivity without affecting embryo quality.
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clinical evidence for a detrimental effect on uterine receptivity of high serum Oestradiol concentrations in high and normal responder patients
Human Reproduction, 1995Co-Authors: Carlos Simón, Fidel Cano, Diana Valbuena, J Remohi, Antonio PellicerAbstract:This study was undertaken to investigate an empirical observation that 'high responder patients have poorer in-vitro fertilization (IVF) outcome than normal responder patients'. The aim of our study was to analyse the effect of high serum Oestradiol and progesterone concentrations at the day of human chorionic gonadotrophin (HCG) administration on endometrial receptivity and oocyte-embryo quality in high and normal responder patients. The IVF patients were divided into two groups: 59 high responder patients who voluntarily donated some of their oocytes, and a control group consisting of 105 normal responder patients. Both groups were compared in terms of the number and quality of oocytes retrieved, embryos transferred, fertilization, implantation and gestation rates, serum Oestradiol and progesterone concentrations and the Oestradiol:progesterone ratio on the day of HCG injection. To ascertain oocyte-embryo quality, a second control group of 96 women undergoing oocyte donation (receiving oocytes from high responder patients) was considered. To assess the impact of steroid concentrations on endometrial receptivity, high responder patients were divided into two subgroups according to Oestradiol concentration, above or below the minimal Oestradiol and progesterone concentrations (mean--SD) in this group. The normal responder patients were divided into two subgroups according to Oestradiol concentration, above or below the maximal Oestradiol and progesterone concentrations (mean+SD) in this group. To assess further the relevance of Oestradiol concentration on endometrial receptivity, patients were divided into different subgroups according to increasing Oestradiol concentration, regardless of whether they were high or normal responders. High responder patients had significantly decreased implantation and pregnancy rates per cycle compared with normal responder patients (33.3 versus 16.3 and 11.1 versus 5.4% respectively; P 1700 pg/ml compared with those having Oestradiol concentrations 2200 pg/ml compared with those having Oestradiol concentrations 2500 pg/ml compared with patients having lower Oestradiol concentrations. Our clinical results demonstrate that high serum Oestradiol concentrations on the day of HCG injection in high and normal responder patients, regardless of the number of oocytes retrieved and the serum progesterone concentration, are detrimental to uterine receptivity without affecting embryo quality.
Marianne Horby Jorgensen - One of the best experts on this subject based on the ideXlab platform.
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oestrogenic potencies of zeranol Oestradiol diethylstilboestrol bisphenol a and genistein implications for exposure assessment of potential endocrine disrupters
Apmis, 2001Co-Authors: Henrik Leffers, Michael Naesby, Brian Vendelbo, Marianne Horby JorgensenAbstract:We have compared the oestrogenic potency of the synthetic oestrogen Zeranol, used as a growth promoter in meat production, and five related compounds, with the potency of 17β-Oestradiol, diethylstilboestrol (DES), genistein, and Bisphenol-A. The potency was assayed by analysing differences in expression levels of endogenous oestrogen-regulated genes in human MCF7 cells, treated with different concentrations of the compounds. Zeranol, 17β-Oestradiol and DES were about equally potent, genistein was four to six orders of magnitude less potent than 17β-Oestradiol but an order of magnitude more potent than Bisphenol-A. There were gene specific differences, the PS2 and TGFβ3 genes were about equally sensitive to Zeranol, 17β-Oestradiol and DES whereas a down-regulation of MRG1/p35srj could be detected at fmol/l concentrations of Zeranol whereas 17β-oestradioI was several orders of magnitude less potent. GST mu3 was sensitive to fmol/l concentrations of 17β-Oestradiol but much less sensitive to Zeranol and DES. The very high potency of Zeranol compared with other potential endocrine disrupters suggests that Zeranol intake from beef products could have greater impact on consumers than the amounts of the known or suspected endocrine disrupters that have been found in food. Since little data is available in man, there is an urgent need for reliable measurements of the concentration of Zeranol in human serum after ingestion of meat products from treated animals.
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oestrogenic potencies of zeranol Oestradiol diethylstilboestrol bisphenol a and genistein implications for exposure assessment of potential endocrine disrupters
Human Reproduction, 2001Co-Authors: Henrik Leffers, Michael Naesby, Brian Vendelbo, Marianne Horby JorgensenAbstract:We have compared the oestrogenic potency of the synthetic oestrogen Zeranol, used as a growth promoter in meat production, and five related compounds, with the potency of 17beta-Oestradiol, diethylstilboestrol (DES), genistein, and Bisphenol-A. The potency was assayed by analysing differences in expression levels of endogenous oestrogen-regulated genes in human MCF7 cells, treated with different concentrations of the compounds. Zeranol, 17beta-Oestradiol and DES were about equally potent, genistein was four to six orders of magnitude less potent than 17beta-Oestradiol but an order of magnitude more potent than Bisphenol-A. There were gene specific differences, the PS2 and TGFbeta3 genes were about equally sensitive to Zeranol, 17beta-Oestradiol and DES whereas a down-regulation of MRG1/p35srj could be detected at fmol/l concentrations of Zeranol whereas 17beta-Oestradiol was several orders of magnitude less potent. GST mu3 was sensitive to fmol/l concentrations of 17beta-Oestradiol but much less sensitive to Zeranol and DES. The very high potency of Zeranol compared with other potential endocrine disrupters suggests that Zeranol intake from beef products could have greater impact on consumers than the amounts of the known or suspected endocrine disrupters that have been found in food. Since little data is available in man, there is an urgent need for reliable measurements of the concentration of Zeranol in human serum after ingestion of meat products from treated animals.
J Remohi - One of the best experts on this subject based on the ideXlab platform.
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endometrial thickness and serum Oestradiol concentrations as predictors of outcome in oocyte donation
Human Reproduction, 1997Co-Authors: J Remohi, Carlos Simón, Gerardo Ardiles, J A Garciavelasco, P Gaitan, A PellicerAbstract:Adequate endometrial preparation with exogenous steroids is mandatory for successful ovum donation. This study was undertaken to assess the value of endometrial thickness by ultrasound and serum Oestradiol as predictors of ovum donation outcome and to analyse the correlation between serum Oestradiol concentrations and the endometrial thickness. Endometrial thickness and serum Oestradiol concentrations on the day of oocyte donation were recorded and compared to several in-vitro fertilization parameters. The cycles (n = 465) were classified according to serum Oestradiol values and endometrial thickness. Comparison of the groups showed that endometrial thickness was significantly (P = 0.002) higher when serum Oestradiol was >400 pg/ml as compared to <100 pg/ml. Pregnancy and implantation rates did not differ among the groups, women with serum Oestradiol <50 pg/ml having similar outcome to the remaining cases. Endometrial thickness showed a similar picture in terms of pregnancy and implantation. Also, women with an endometrium <4 mm in size had normal pregnancy and implantation rates. There was a positive correlation (P = 0.0044) between endometrial thickness and implantation, as well as between endometrial thickness and serum Oestradiol (P = 0.0184). None of the parameters examined was able to predict ovum donation outcome. It is concluded that endometrial thickness is preferred to serum Oestradiol for the monitoring of endometrial development, although neither is able to predict success in oocyte donation.
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endometrial thickness and serum Oestradiol concentrations as predictors of outcome in oocyte donation
Human Reproduction, 1997Co-Authors: J Remohi, Carlos Simón, Gerardo Ardiles, J A Garciavelasco, P Gaitan, A PellicerAbstract:Adequate endometrial preparation with exogenous steroidsis mandatory for successful ovum donation. This study wasundertaken to assess the value of endometrial thickness byultrasound and serum Oestradiol as predictors of ovumdonation outcome and to analyse the correlation betweenserum Oestradiol concentrations and the endometrial thick-ness. Endometrial thickness and serum Oestradiol concen-trations on the day of oocyte donation were recorded andcompared to several in-vitro fertilization parameters. Thecycles (n J 465) were classified according to serum oest-radiol values and endometrial thickness. Comparison ofthe groups showed that endometrial thickness was signi-ficantly (P J 0.002) higher when serum Oestradiol was
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clinical evidence for a detrimental effect on uterine receptivity of high serum Oestradiol concentrations in high and normal responder patients
Human Reproduction, 1995Co-Authors: Carlos Simón, Fidel Cano, Diana Valbuena, J Remohi, Antonio PellicerAbstract:This study was undertaken to investigate an empirical observation that 'high responder patients have poorer in-vitro fertilization (IVF) outcome than normal responder patients'. The aim of our study was to analyse the effect of high serum Oestradiol and progesterone concentrations at the day of human chorionic gonadotrophin (HCG) administration on endometrial receptivity and oocyte-embryo quality in high and normal responder patients. The IVF patients were divided into two groups: 59 high responder patients who voluntarily donated some of their oocytes, and a control group consisting of 105 normal responder patients. Both groups were compared in terms of the number and quality of oocytes retrieved, embryos transferred, fertilization, implantation and gestation rates, serum Oestradiol and progesterone concentrations and the Oestradiol:progesterone ratio on the day of HCG injection. To ascertain oocyte-embryo quality, a second control group of 96 women undergoing oocyte donation (receiving oocytes from high responder patients) was considered. To assess the impact of steroid concentrations on endometrial receptivity, high responder patients were divided into two subgroups according to Oestradiol concentration, above or below the minimal Oestradiol and progesterone concentrations (mean--SD) in this group. The normal responder patients were divided into two subgroups according to Oestradiol concentration, above or below the maximal Oestradiol and progesterone concentrations (mean+SD) in this group. To assess further the relevance of Oestradiol concentration on endometrial receptivity, patients were divided into different subgroups according to increasing Oestradiol concentration, regardless of whether they were high or normal responders. High responder patients had significantly decreased implantation and pregnancy rates per cycle compared with normal responder patients (33.3 versus 16.3 and 11.1 versus 5.4% respectively; P 1700 pg/ml compared with those having Oestradiol concentrations 2200 pg/ml compared with those having Oestradiol concentrations 2500 pg/ml compared with patients having lower Oestradiol concentrations. Our clinical results demonstrate that high serum Oestradiol concentrations on the day of HCG injection in high and normal responder patients, regardless of the number of oocytes retrieved and the serum progesterone concentration, are detrimental to uterine receptivity without affecting embryo quality.
David E. Wildt - One of the best experts on this subject based on the ideXlab platform.
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excretion rates and metabolites of Oestradiol and progesterone in baboon papio cynocephalus cynocephalus faeces
Reproduction, 1994Co-Authors: Samuel K. Wasser, J Southers, Steven L. Monfort, David E. WildtAbstract:: Two unanaesthetized female yellow baboons (Papio cynocephalus cynocephalus) were infused (i.v.) with [3H]Oestradiol and two with [3H]progesterone, early in the follicular phases of their cycles. One month later, the two females infused with [3H]Oestradiol were simultaneously infused with [14C]progesterone and [3H]dehydroepiandrosterone. All urine and faeces were collected for 96 h after infusion. The proportion of steroid excreted in faeces (versus urine) was 10.0% for Oestradiol and 40% for progesterone. Peak excretion in urine occurred 4.5 h after infusion. Peak excretion in faeces occurred an average of 36.4 h after infusion, with remarkable consistency between steroids. Eighty per cent of faecal Oestradiol and progesterone metabolites were excreted as free (rather than conjugated) steroids. Simply boiling (20 min) the dried faecal sample in 90% ethanol proved to be the most rapid and efficient means of extracting these steroid metabolites. High pressure liquid chromatography and immunoreactivity studies revealed that Oestradiol was excreted in faeces as Oestradiol (36%), oestrone (44%) and a conjugated metabolite that co-eluted with oestrone sulfate (20%). Progesterone was excreted as eight different free forms, only a minor portion of which was progesterone, and what appeared to be a conjugated metabolite that co-eluted with pregnanediol-glucuronide (20%). The free progesterone metabolites were identified by gas-chromatography-mass-spectrometry as epimers of 5-pregnane-3-diol and 5-pregnane-3-ol-one. These data suggest that currently available immunoassays for free Oestradiol and oestrone should adequately characterize faecal oestrogen profiles in baboons. However, high variability in crossreactivities of various progesterone antisera to progesterone metabolites in baboons makes antiserum selection a more serious concern in attempts to quantify faecal progestogen dynamics.(ABSTRACT TRUNCATED AT 250 WORDS)
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rapid extraction of faecal steroids for measuring reproductive cyclicity and early pregnancy in free ranging yellow baboons papio cynocephalus cynocephalus
Reproduction, 1991Co-Authors: Samuel K. Wasser, Steven L. Monfort, David E. WildtAbstract:A rapid method was developed for extracting and assaying Oestradiol and progesterone in faeces (n = 242) of female yellow baboons, free-living in Tanzania. Dose response studies generated slopes of 1.02 (r2 = 0.99) for Oestradiol and 1.09 (r2 = 0.99) for progesterone, suggesting that this method accurately measured these steroids in faeces. Parallelism was proved by demonstrating that slopes produced from serially diluted samples were not different from those generated from standard curves (mean P value = 0.53 +/- 0.17 for Oestradiol and 0.44 +/- 0.13 for progesterone). Faecal progesterone concentrations measured over several cycles in 2 females increased and decreased in correspondence to visual markers of the luteal phase (i.e. the period between sex-skin detumescence and menses), but the presumed preovulatory Oestradiol peak was not observed consistently in all cycles. Progesterone profiles during early to midgestation in 3 females confirmed pregnancy by 25 days (14%) of gestation. Oestradiol profiles were more variable and were not indicative of pregnancy until 40 days (22%) of gestation. Radiolabel-infusion studies revealed that 32% of progesterone (n = 2) but only 11% of Oestradiol (n = 2), was cleared through faeces. The latter findings may account for the greater variation observed in temporal Oestradiol patterns during the baboon menstrual cycle and pregnancy. Compared with previous techniques, these new methods (i) save considerable time in assaying raw material and (ii) result in high extraction recoveries of faecal steroids (approximately 88% for Oestradiol and 91% for progesterone). This approach may be particularly useful for studying physiological function and endocrine-environmental interrelationships in free-living primate species.
