The Experts below are selected from a list of 312 Experts worldwide ranked by ideXlab platform
P Vijayagopal - One of the best experts on this subject based on the ideXlab platform.
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evaluation of dietary Oleoresins on the enhancement of skin coloration and growth in the marine ornamental clown fish amphiprion ocellaris cuvier 1830
Aquaculture, 2020Co-Authors: Sanal Ebeneezar, Linga D Prabu, S Chandrasekar, C S Tejpal, K Madhu, P Sayooj, P VijayagopalAbstract:Abstract A 60-day feeding trial was carried out to investigate the effects of dietary oleoresin inclusion on skin colour intensity, carotenoid content, growth performance and digestive enzyme activities of a high value marine ornamental clown fish, A. ocellaris. Experimental diets were formulated with natural oleoresin pigments namely, paprika, turmeric and chlorophyll, with dietary inclusion at the rate of 20 gkg−1 in the diets. Experimental units were set in triplicates with six numbers of fish in each tank and maintained in filtered sea water of 20‰ salinity in a recirculatory system. Clown fish with average initial weight of 150.6 ± 0.10 mg and initial length of 8 mm were fed five different diets viz., control/ basal diet (C), diets with inclusion at the rate of 20 gkg−1 each of paprika (PAP), turmeric (TUR) and chlorophyll (CHL) Oleoresins and combination of Oleoresins (COM) at the rate of 20 gkg−1. Skin colour intensity in terms of red and yellow hues and total carotenoid levels differed significantly (P .05) with respect to feed efficiency, digestive enzyme activities, viscero somatic index, visceral length index, condition factor and whole body proximate composition among different treatments. These results suggested that the dietary pigments have a direct influence on the skin pigmentation in A. ocellaris without adverse effects on digestive functions and body condition. Colour imparted in fish skin due to paprika oleoresin was found to be superior.
Sanal Ebeneezar - One of the best experts on this subject based on the ideXlab platform.
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evaluation of dietary Oleoresins on the enhancement of skin coloration and growth in the marine ornamental clown fish amphiprion ocellaris cuvier 1830
Aquaculture, 2020Co-Authors: Sanal Ebeneezar, Linga D Prabu, S Chandrasekar, C S Tejpal, K Madhu, P Sayooj, P VijayagopalAbstract:Abstract A 60-day feeding trial was carried out to investigate the effects of dietary oleoresin inclusion on skin colour intensity, carotenoid content, growth performance and digestive enzyme activities of a high value marine ornamental clown fish, A. ocellaris. Experimental diets were formulated with natural oleoresin pigments namely, paprika, turmeric and chlorophyll, with dietary inclusion at the rate of 20 gkg−1 in the diets. Experimental units were set in triplicates with six numbers of fish in each tank and maintained in filtered sea water of 20‰ salinity in a recirculatory system. Clown fish with average initial weight of 150.6 ± 0.10 mg and initial length of 8 mm were fed five different diets viz., control/ basal diet (C), diets with inclusion at the rate of 20 gkg−1 each of paprika (PAP), turmeric (TUR) and chlorophyll (CHL) Oleoresins and combination of Oleoresins (COM) at the rate of 20 gkg−1. Skin colour intensity in terms of red and yellow hues and total carotenoid levels differed significantly (P .05) with respect to feed efficiency, digestive enzyme activities, viscero somatic index, visceral length index, condition factor and whole body proximate composition among different treatments. These results suggested that the dietary pigments have a direct influence on the skin pigmentation in A. ocellaris without adverse effects on digestive functions and body condition. Colour imparted in fish skin due to paprika oleoresin was found to be superior.
Jairo Kenupp Bastos - One of the best experts on this subject based on the ideXlab platform.
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reliable methods for analyses of volatile compounds of copaifera Oleoresins combining headspace and gas chromatography
Chemistry & Biodiversity, 2020Co-Authors: Jonas Joaquim Mangabeira Da Silva, Victor Pena Ribeiro, Herve Rogez, Marivane Lemos, Antonio Eduardo Miller Crotti, Jairo Kenupp BastosAbstract:Two analytical methods were developed in this study for direct and fast chemical investigation of authentic Copaifera Oleoresins (COR) and commercial products. Polydimethylsiloxane microfiber coupled to gas chromatography-mass spectrometry (HS-SPME-GC/MS) showed the best results for oleoresin qualitative analysis, setting the following extraction conditions: equilibrium time of 15 min, extraction time of 30 min, extraction temperature at 60 °C and constant stirring of 400 rpm. Sesquiterpenes α-copaene, β-elemene, β-caryophyllene and trans-α-bergamotene were found in all investigated samples. Quantitative analysis by gas chromatography coupled with flame ionization detector (GC-FID) measured the content of the four sesquiterpenes in all samples. Qualitative and quantitative results showed important differences between COR of distinct species and commercial products. Data regarding the volatile composition of C. oblongifolia and C. trapezifolia Oleoresins were first presented in this study and two new analytical methods were reported for direct and fast qualitative and quantitative analysis of COR.
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use of spinning band distillation equipment for fractionation of volatile compounds of copaifera Oleoresins for developing a validated gas chromatographic method and evaluating antimicrobial activity
Biomedical Chromatography, 2019Co-Authors: Victor Pena Ribeiro, Caroline Arruda, Jonas Joaquim Mangabeira Da Silva, Jennyfer Andrea Aldana Mejia, Niege Aracari Jacometti Cardoso Furtado, Jairo Kenupp BastosAbstract:Copaifera is a tree that produces an oleoresin that has great historical and economic importance. These Oleoresins display several pharmacological properties, such as anti-inflammatory and antimicrobial, among others. The commercialization of Copaifera oleoresin occurs, in many cases, without any quality control, which facilitates its adulteration. Validated analytical methods can provide a safe quality control. In this work, the 800 Automatic Spinning Band Distillation equipment was used to perform the fractionation of the volatile oils obtained by hydrodistillation of Copaifera multijuga, C. paupera, C. Publifora and C. langsdorffii, aiming to isolate and purify the major compounds present in these oils. For purification, classical column chromatography was used, furnishing six isolated sesquiterpenes. The sesquiterpenes were used as standards in the development and validation of the method by GC-FID. The evaluated parameters were selectivity, linearity, precision, accuracy and robustness and they are all in accordance with ANVISA and International Conference on Harmonization guidelines. The developed method is reliable for the quantification of sesquiterpenes in Copaifera Oleoresins. Both volatile oils and isolated sesquiterpenes had their minimum inhibitory concentration determined against strains of Gram-negative and Gram-positive bacteria and yeasts. Copaifera langsdorffi oleoresin was the only one active against all of the evaluated microorganisms, displaying good antimicrobial potential.
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in vitro studies of the antibacterial activity of copaifera spp Oleoresins sodium hypochlorite and peracetic acid against clinical and environmental isolates recovered from a hemodialysis unit
Antimicrobial Resistance and Infection Control, 2018Co-Authors: Rosimara G L Vieira, Thais Da Silva Moraes, Larissa De Oliveira Silva, Thamires Chiquini Bianchi, Rodrigo Cassio Sola Veneziani, Sergio R Ambrosio, Jairo Kenupp Bastos, Regina Helena Pires, Carlos Henrique Gomes MartinsAbstract:Patients submitted to hemodialysis therapy are more susceptible to infection, especially to infection by Gram-positive bacteria. Various research works have attempted to discover new antimicrobial agents from plant extracts and other natural products. The present study aimed to assess the antibacterial activities of Copaifera duckei, C. reticulata, and C. oblongifolia Oleoresins; sodium hypochlorite; and peracetic acid against clinical and environmental isolates recovered from a Hemodialysis Unit. The Minimum Inhibitory Concentration and the Fractionated Inhibitory Concentration Index were determined; the ability of the tested compounds/extracts to inhibit biofilm formation was evaluated by calculating the MICB50 and IC50. C. duckei was the most efficient among the assayed Copaifera species, and its oleoresin was more effective than peracetic acid and sodium hypochlorite. Copaifera Oleoresins and disinfectants did not act synergistically at any of the tested combinations. Certain of C. duckei oleoresin, peracetic acid, and sodium hypochlorite concentrations inhibited biofilm formation and eradicated 50% of the biofilm population. C. duckei oleoresin is a potential candidate for disinfectant formulations. Based on these results and given the high incidence of multi-resistant bacteria in hemodialysis patients, it is imperative that new potential antibacterial agents like C. duckei oleoresin, which is active against Staphylococcus, be included in disinfectant formulations.
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In vitro studies of the antibacterial activity of Copaifera spp. Oleoresins, sodium hypochlorite, and peracetic acid against clinical and environmental isolates recovered from a hemodialysis unit
BMC, 2018Co-Authors: Rosimara G L Vieira, Thais Da Silva Moraes, Larissa De Oliveira Silva, Thamires Chiquini Bianchi, Rodrigo Cassio Sola Veneziani, Sergio R Ambrosio, Jairo Kenupp Bastos, Regina Helena Pires, Carlos Henrique Gomes MartinsAbstract:Abstract Background Patients submitted to hemodialysis therapy are more susceptible to infection, especially to infection by Gram-positive bacteria. Various research works have attempted to discover new antimicrobial agents from plant extracts and other natural products. Methods The present study aimed to assess the antibacterial activities of Copaifera duckei, C. reticulata, and C. oblongifolia Oleoresins; sodium hypochlorite; and peracetic acid against clinical and environmental isolates recovered from a Hemodialysis Unit. The Minimum Inhibitory Concentration and the Fractionated Inhibitory Concentration Index were determined; the ability of the tested compounds/extracts to inhibit biofilm formation was evaluated by calculating the MICB50 and IC50. Results C. duckei was the most efficient among the assayed Copaifera species, and its oleoresin was more effective than peracetic acid and sodium hypochlorite. Copaifera Oleoresins and disinfectants did not act synergistically at any of the tested combinations. Certain of C. duckei oleoresin, peracetic acid, and sodium hypochlorite concentrations inhibited biofilm formation and eradicated 50% of the biofilm population. Conclusion C. duckei oleoresin is a potential candidate for disinfectant formulations. Based on these results and given the high incidence of multi-resistant bacteria in hemodialysis patients, it is imperative that new potential antibacterial agents like C. duckei oleoresin, which is active against Staphylococcus, be included in disinfectant formulations
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development of a validated ultra high performance liquid chromatography tandem mass spectrometry method for determination of acid diterpenes in copaifera Oleoresins
Journal of Chromatography A, 2017Co-Authors: Jonas Joaquim Mangabeira Da Silva, Rodrigo Cassio Sola Veneziani, Luiza J. Carneiro, Sérgio R. Ambrósio, Eduardo J Crevelin, Herve Rogez, Luiz Alberto Beraldo De Moraes, Jairo Kenupp BastosAbstract:Abstract Species of Copaifera genus (Fabaceae – Caesalpinoiodidaeae) produces an important commercial oleoresin that displays many medicinal properties. Copaifera Oleoresins (COR) are composed mainly of a mixture of diterpenes and sequiterpenes, and the main reported acid diterpenes for this genus are kaurenoic, copalic, hardwickiic and polyaltic acids. An ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for identification and quantification of nine acid diterpenes. The developed method was applied in the analyses of 10 authentic COR samples collected in the North and Southeast of Brazil and six commercial COR samples. Samples preparation consisted of simple dilution of Oleoresins in methanol followed by filtration. Validation parameters of the method for nine acid diterpenes were satisfactory: selectivity/specificity was defined by retention time and MS/MS analyses for each analyte; generally all analytical curves presented r2 > 0.99, Lack-of-fit test not significant and RSD
C S Tejpal - One of the best experts on this subject based on the ideXlab platform.
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evaluation of dietary Oleoresins on the enhancement of skin coloration and growth in the marine ornamental clown fish amphiprion ocellaris cuvier 1830
Aquaculture, 2020Co-Authors: Sanal Ebeneezar, Linga D Prabu, S Chandrasekar, C S Tejpal, K Madhu, P Sayooj, P VijayagopalAbstract:Abstract A 60-day feeding trial was carried out to investigate the effects of dietary oleoresin inclusion on skin colour intensity, carotenoid content, growth performance and digestive enzyme activities of a high value marine ornamental clown fish, A. ocellaris. Experimental diets were formulated with natural oleoresin pigments namely, paprika, turmeric and chlorophyll, with dietary inclusion at the rate of 20 gkg−1 in the diets. Experimental units were set in triplicates with six numbers of fish in each tank and maintained in filtered sea water of 20‰ salinity in a recirculatory system. Clown fish with average initial weight of 150.6 ± 0.10 mg and initial length of 8 mm were fed five different diets viz., control/ basal diet (C), diets with inclusion at the rate of 20 gkg−1 each of paprika (PAP), turmeric (TUR) and chlorophyll (CHL) Oleoresins and combination of Oleoresins (COM) at the rate of 20 gkg−1. Skin colour intensity in terms of red and yellow hues and total carotenoid levels differed significantly (P .05) with respect to feed efficiency, digestive enzyme activities, viscero somatic index, visceral length index, condition factor and whole body proximate composition among different treatments. These results suggested that the dietary pigments have a direct influence on the skin pigmentation in A. ocellaris without adverse effects on digestive functions and body condition. Colour imparted in fish skin due to paprika oleoresin was found to be superior.
K Madhu - One of the best experts on this subject based on the ideXlab platform.
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evaluation of dietary Oleoresins on the enhancement of skin coloration and growth in the marine ornamental clown fish amphiprion ocellaris cuvier 1830
Aquaculture, 2020Co-Authors: Sanal Ebeneezar, Linga D Prabu, S Chandrasekar, C S Tejpal, K Madhu, P Sayooj, P VijayagopalAbstract:Abstract A 60-day feeding trial was carried out to investigate the effects of dietary oleoresin inclusion on skin colour intensity, carotenoid content, growth performance and digestive enzyme activities of a high value marine ornamental clown fish, A. ocellaris. Experimental diets were formulated with natural oleoresin pigments namely, paprika, turmeric and chlorophyll, with dietary inclusion at the rate of 20 gkg−1 in the diets. Experimental units were set in triplicates with six numbers of fish in each tank and maintained in filtered sea water of 20‰ salinity in a recirculatory system. Clown fish with average initial weight of 150.6 ± 0.10 mg and initial length of 8 mm were fed five different diets viz., control/ basal diet (C), diets with inclusion at the rate of 20 gkg−1 each of paprika (PAP), turmeric (TUR) and chlorophyll (CHL) Oleoresins and combination of Oleoresins (COM) at the rate of 20 gkg−1. Skin colour intensity in terms of red and yellow hues and total carotenoid levels differed significantly (P .05) with respect to feed efficiency, digestive enzyme activities, viscero somatic index, visceral length index, condition factor and whole body proximate composition among different treatments. These results suggested that the dietary pigments have a direct influence on the skin pigmentation in A. ocellaris without adverse effects on digestive functions and body condition. Colour imparted in fish skin due to paprika oleoresin was found to be superior.
