The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Hans Clevers - One of the best experts on this subject based on the ideXlab platform.
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patient derived Oral Mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
PLOS ONE, 2020Co-Authors: Else Driehuis, Natanja Oosterom, Sandra G Heil, I B Muller, Marry Lin, S Kolders, Gerrit Jansen, R De Jonge, R Pieters, Hans CleversAbstract:We have recently established a protocol to grow wildtype human Oral Mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the Oral Mucosa. Here, we validate the use of this model to study the effect of Leucovorin (LV) on Methotrexate (MTX)-induced toxicity. MTX is a chemotherapeutic agent used in the treatment of pediatric acute lymphoblastic leukemia. Although effective, the use of MTX often results in severe side-effects, including Oral mucositis, which is characterized by epithelial cell death. Here, we show that organoids are sensitive to MTX, and that the addition of LV reduces MTX toxicity, in both a concentration- and timing-dependent manner. Additionally, we show that a 24 hour 'pretreatment' with LV reduces MTX-induced cell death, suggesting that such a pretreatment could decrease mucositis in patients. Taken together, we provide the first in vitro model to study the effect of MTX on wildtype Oral Mucosa cells. Our findings underscore the relevance of the clinically applied LV regimen and highlight the potential of this model to further optimize modifications in dosing and timing of Leucovorin on Oral Mucosa cells.
Else Driehuis - One of the best experts on this subject based on the ideXlab platform.
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patient derived Oral Mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
PLOS ONE, 2020Co-Authors: Else Driehuis, Natanja Oosterom, Sandra G Heil, I B Muller, Marry Lin, S Kolders, Gerrit Jansen, R De Jonge, R Pieters, Hans CleversAbstract:We have recently established a protocol to grow wildtype human Oral Mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the Oral Mucosa. Here, we validate the use of this model to study the effect of Leucovorin (LV) on Methotrexate (MTX)-induced toxicity. MTX is a chemotherapeutic agent used in the treatment of pediatric acute lymphoblastic leukemia. Although effective, the use of MTX often results in severe side-effects, including Oral mucositis, which is characterized by epithelial cell death. Here, we show that organoids are sensitive to MTX, and that the addition of LV reduces MTX toxicity, in both a concentration- and timing-dependent manner. Additionally, we show that a 24 hour 'pretreatment' with LV reduces MTX-induced cell death, suggesting that such a pretreatment could decrease mucositis in patients. Taken together, we provide the first in vitro model to study the effect of MTX on wildtype Oral Mucosa cells. Our findings underscore the relevance of the clinically applied LV regimen and highlight the potential of this model to further optimize modifications in dosing and timing of Leucovorin on Oral Mucosa cells.
Martin H Thornhill - One of the best experts on this subject based on the ideXlab platform.
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tissue engineered Oral Mucosa
Journal of Dental Research, 2012Co-Authors: Keyvan Moharamzadeh, Helen E Colley, Craig Murdoch, Vanessa Hearnden, Wen Lin Chai, I M Brook, Martin H Thornhill, Sheila MacneilAbstract:Advances in tissue engineering have permitted the three-dimensional (3D) reconstruction of human Oral Mucosa for various in vivo and in vitro applications. Tissue-engineered Oral Mucosa have been further optimized in recent years for clinical applications as a suitable graft material for intra-Oral and extra-Oral repair and treatment of soft-tissue defects. Novel 3D in vitro models of Oral diseases such as cancer, Candida, and bacterial invasion have been developed as alternatives to animal models for investigation of disease phenomena, their progression, and treatment, including evaluation of drug delivery systems. The introduction of 3D Oral Mucosal reconstructs has had a significant impact on the approaches to biocompatibility evaluation of dental materials and Oral healthcare products as well as the study of implant-soft tissue interfaces. This review article discusses the recent advances in tissue engineering and applications of tissue-engineered human Oral Mucosa.
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the effect of sodium lauryl sulphate triclosan and zinc on the permeability of normal Oral Mucosa
Oral Diseases, 2008Co-Authors: Claire M Healy, Martin H Thornhill, A T Cruchley, D M WilliamsAbstract:OBJECTIVE: Sodium lauryl sulphate (SLS), an important component in many Oral health products, is well established as a contact irritant in skin. Recent studies have suggested that it may also affect the structural integrity of Oral Mucosa. SLS is rarely used alone in dentifrices or mouthwashes and the aim of this study was to establish the effect of SLS both alone and in combination with Triclosan (TCN) and zinc (Zn) on the permeability barrier properties of normal human Oral Mucosa. METHOD: Ventral tongue Mucosa was obtained from nine males and seven females within 60 h of death and stored frozen at -70 degrees C until use. The permeability of the tissue to tritiated water was measured after pretreatment for 15 min with SLS alone, SLS/TCN, SLS/Zn and a SLS/TCN/Zn mixture. Treatment with distilled water (DW) served as control. The histological appearance of the tissue before and after treatment was also examined by light microscopy. RESULTS: SLS treatment caused a significant increase in water permeability compared to control tissue (Kp = 11.7 +/- 1.00; 4.96 +/- 0.50 respectively; P < 0.005). Treatment with a SLS/TCN/Zn mixture, however, had no effect on the permeability to water (Kp = 5.5 +/- 0.56). Histological examination revealed that tissue exposed to SLS had a marked disruption of the epithelial surface whilst tissue treated with a SLS/TCN/Zn mixture was indistinguishable from controls. CONCLUSION: Although Mucosa exposed to SLS alone showed an increase in permeability to water, the addition of TCN and Zn to SLS appeared to prevent this effect. As SLS is included in some dental products to solubilise compounds such as TCN, its presence may have no effect on the permeability barrier property of Oral Mucosa.
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tissue engineered Oral Mucosa a review of the scientific literature
Journal of Dental Research, 2007Co-Authors: Keyvan Moharamzadeh, I M Brook, R Van Noort, Andy M Scutt, Martin H ThornhillAbstract:Tissue-engineered Oral Mucosal equivalents have been developed for clinical applications and also for in vitro studies of biocompatibility, Mucosal irritation, disease, and other basic Oral biology phenomena. This paper reviews different tissue-engineering strategies used for the production of human Oral Mucosal equivalents, their relative advantages and drawbacks, and their applications. Techniques used for skin tissue engineering that may possibly be used for in vitro reconstruction of human Oral Mucosa are also discussed.
Stina Syrjanen - One of the best experts on this subject based on the ideXlab platform.
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genotype specific incidence and clearance of human papillomavirus in Oral Mucosa of women a six year follow up study
PLOS ONE, 2013Co-Authors: Jaana Rautava, Jaana Willberg, Karolina Louvanto, Lilli Wideman, Kari Syrjanen, Seija Grenman, Stina SyrjanenAbstract:Background There are no previous longitudinal studies on genotype-specific natural history of human papillomavirus (HPV) infections in Oral Mucosa of women.
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human papillomavirus infections in the Oral Mucosa
Journal of the American Dental Association, 2011Co-Authors: Jaana Rautava, Stina SyrjanenAbstract:ABSTRACT Background Public awareness of human papillomavirus (HPV) as the causal agent of cervical cancer and of the availability of HPV vaccines has increased. As a result, more patients are asking their dentists about Oral HPV infection and its prevention by means of vaccination. Parents of pediatric dental patients also may be concerned when their children have HPV-associated Oral lesions, because HPV infection still often is considered a purely sexually transmitted disease. In this review, the authors provide the latest information for dental professionals about HPV infection in the Oral Mucosa and in general. Types of Studies Reviewed The authors searched PubMed for all studies regarding HPV infection in the Oral Mucosa, and they reviewed relevant publications focusing exclusively on HPV infections of the Oral cavity. In selecting studies for review, the authors made a clear distinction between studies regarding HPV infections in the mouth and those regarding HPV infection in the oropharynx or in other head and neck sites. Results HPV can infect Oral Mucosa. A subgroup of Oral cancer clearly is associated with HPV. Oral HPV infection is transmitted sexually but also can be transmitted from mouth to mouth and vertically from an infected mother during delivery. Conclusions and Clinical Implications Persistent HPV infection in the Oral Mucosa might increase the risk of developing Oral cancer. Regular and meticulous clinical examination is the dentist's most important tool in detecting HPV-associated changes in the Oral Mucosae. HPV-associated Oral cancer may affect a population younger than that typically affected by HPV-independent Oral cancer. Alcohol and tobacco use increase the risk of developing Oral cancer, so good practice includes encouraging patients to avoid these habits. The availabel HPV vaccines cover the HPV genotypes found most commonly in the Oral Mucosa, but their protective effect against Oral cancer remains to be elucidated.
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marginal periodontium as a potential reservoir of human papillomavirus in Oral Mucosa
Journal of Periodontology, 2005Co-Authors: Marketta Hormia, Jaana Willberg, Hellevi Ruokonen, Stina SyrjanenAbstract:Background: Although human papillomaviruses (HPVs) are associated with a number of proliferative epithelial lesions including squamous cell malignancies, they can also be detected in the normal Oral Mucosa in 10% to 20% of the adult population. However, the point of entry and the site of replication of HPV in the Oral cavity are not known. Since the gingival pocket is the only site in the Oral Mucosa where basal cells, known to be targets of HPV at other Mucosal sites, are normally exposed to the environment, we hypothesized that this could be the site of latent HPV. Methods: Gingival biopsies taken from 38 individuals with clinically diagnosed periodontal disease were examined. The presence of HPV DNA was studied by using nested PCR (polymerase chain reaction with MY09/MY11 and GP05+/GP06+ primers targeting the L1 region of HPV), followed by subsequent hybridization with a cocktail of 12 high-risk HPV oligoprobes and in situ hybridization (ISH) with probes for HPV screening and the HPV subtype 16. Result...
R De Jonge - One of the best experts on this subject based on the ideXlab platform.
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patient derived Oral Mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
PLOS ONE, 2020Co-Authors: Else Driehuis, Natanja Oosterom, Sandra G Heil, I B Muller, Marry Lin, S Kolders, Gerrit Jansen, R De Jonge, R Pieters, Hans CleversAbstract:We have recently established a protocol to grow wildtype human Oral Mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the Oral Mucosa. Here, we validate the use of this model to study the effect of Leucovorin (LV) on Methotrexate (MTX)-induced toxicity. MTX is a chemotherapeutic agent used in the treatment of pediatric acute lymphoblastic leukemia. Although effective, the use of MTX often results in severe side-effects, including Oral mucositis, which is characterized by epithelial cell death. Here, we show that organoids are sensitive to MTX, and that the addition of LV reduces MTX toxicity, in both a concentration- and timing-dependent manner. Additionally, we show that a 24 hour 'pretreatment' with LV reduces MTX-induced cell death, suggesting that such a pretreatment could decrease mucositis in patients. Taken together, we provide the first in vitro model to study the effect of MTX on wildtype Oral Mucosa cells. Our findings underscore the relevance of the clinically applied LV regimen and highlight the potential of this model to further optimize modifications in dosing and timing of Leucovorin on Oral Mucosa cells.
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Patient-derived Oral Mucosa organoids as an in vitro model for methotrexate induced toxicity in pediatric acute lymphoblastic leukemia
'Public Library of Science (PLoS)', 2020Co-Authors: Driehuis E., R De Jonge, Oosterom N., Heil S.g., Muller, I.b. B.), Lin M., Kolders S., Jansen G., Pieters R., Clevers H.c.Abstract:We have recently established a protocol to grow wildtype human Oral Mucosa organoids. These three-dimensional structures can be maintained in culture long-term, do not require immortalization, and recapitulate the multilayered composition of the epithelial lining of the Oral Mucosa. Here, we validate the use of this model to study the effect of Leucovorin (LV) on Methotrexate (MTX)-induced toxicity. MTX is a chemotherapeutic agent used in the treatment of pediatric acute lymphoblastic leukemia. Although effective, the use of MTX often results in severe side-effects, including Oral mucositis, which is characterized by epithelial cell death. Here, we show that organoids are sensitive to MTX, and that the add
