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Jerzy Falandysz - One of the best experts on this subject based on the ideXlab platform.

  • Selenium and 17 other largely essential and toxic metals in muscle and Organ Meats of Red Deer (Cervus elaphus) — Consequences to human health
    Environment international, 2011
    Co-Authors: Grażyna Jarzyńska, Jerzy Falandysz
    Abstract:

    Concentrations, composition and interrelationships of selenium and metallic elements (Ag, Ba, Cd, Co, Cr, Cs, Cu, Ga, Mn, Mo, Pb, Rb, Sb, Sr, Tl, V and Zn) have been examined in muscle and Organ Meats of Red Deer hunted in Poland. The analytical data obtained were also discussed in terms of Se supplementation and deficit to Deer as well as the benefits and risk to humans associated with the essential and toxic metals intake resulting from consumption of Deer meat and products. These elements were determined in 20 adult animals of both sexes that were obtained in the 2000/2001 hunting season from Warmia and Mazury in the north-eastern part of Poland. The whole kidneys contained Ba, Cd, Cr, Ga, Pb, Se, Sr and Tl at statistically greater concentrations than liver or muscle tissue from the same animal. Liver showed statistically greater concentrations of Ag, Co, Cu, Mn and Mo than kidneys or muscle tissue, and muscle tissue was richer in Zn, when compared to the kidneys or liver. Cs and Rb were similarly distributed between all three tissue types, while V was less abundant in liver than kidneys or muscle tissue. There were significant associations between some metallic elements retained in Red Deer demonstrated by Principal Component Analysis (PCA) of the data set. In Organ and muscle Meats (kidneys, liver and muscle tissue considered together) the first principal component (PC1) was strongly influenced by positively correlated variables describing Se, Ba and Cd and negatively correlated variables describing Ag, Co, Cs, Mn, Pb, Tl and V; PC2, respectively, by Cu, Mn and Mo (+) and Zn (-); PC3 by Ga (+) and PC4 by Sb (+). Selenium occurred in muscle tissue, liver and kidneys at median concentrations of 0.13, 0.19 and 4.0mg/g dry weight, respectively. These values can be defined as marginally deficient (< 0.6mg Se/kg liver dw) or satisfactory (≤ 3.0mg Se/kg kidneys dw) for the amount required to maintain the Deer's body condition and health, depending on the criterion for supplementation used. In terms of human nutritional needs, a relatively high selenium content of kidneys can be beneficial. The muscle meat, liver and kidneys of Red Deer can be considered as a very good source of essential Co, Cr, Cu, Mo, Mn, Se and Zn in the human diet. Lead is generally considered as toxic, and the concentrations found in Red Deer (via the food chain intake) were well below the European Union tolerance limit. Pb from the lead bullets can always create food hygienic problem, if not well recognized during sanitary inspection, and this was noted for one muscle meat sample in this study (5% surveyed). There is no tolerance limit of Cd in game animal Meats. The median values of Cd noted in fresh muscle tissue, liver and whole kidneys were 0.07, 0.18, and 3.3mg/kg wet weight, respectively. Cd exists as a chemical element present at trace levels in plants and mushrooms in Deer's food chain in background (uncontaminated) areas. When these are consumed by the Deer, the amount of Cd sequestered with metallothioneins and retained in the Organ and muscle meat in this study is low enough to be considered safe for human consumption.

  • Mercury, lead, cadmium, manganese, copper, iron and zinc concentrations in poultry, rabbit and sheep from the northern part of Poland
    The Science of the total environment, 1994
    Co-Authors: Jerzy Falandysz, Wanda Kotecka, Kurunthachalam Kannan
    Abstract:

    The concentrations of mercury, lead, cadmium, manganese, copper, iron and zinc have been determined in poultry, rabbit and sheep from the northern part of Poland between 1988 and 1991. The method of measurement was cold vapour flameless AAS for mercury and flame AAS for other trace metals. Rabbits contained somewhat higher concentrations of lead in liver and kidneys than other species examined and the same could be observed in the case of cadmium in Organ Meats of duck and geese with apparently higher contamination of the rabbit's kidneys. While for an essential trace metals the values obtained seem to be species/tissue specific and typical.

  • Some toxic and essential trace metals in swine from Northern Poland
    The Science of the total environment, 1993
    Co-Authors: Jerzy Falandysz
    Abstract:

    Abstract The concentrations of mercury, cadmium, lead, copper, manganese, zinc and iron have been determined in muscle (meat), liver and kidneys of swine slaughtered in the northern part of Poland between 1987 and 1991. The method of measurement was cold vapour flameless AAS for mercury and flame ASS for other trace metals. The concentrations of such toxic metals as mercury, lead and cadmium were relatively low or very low. The arithmetic mean weighted concentrations obtained related to fresh weight for muscle, liver and kidneys of swine were 1.9, 3.7 and 7.0 μg kg −1 for mercury, 5, 41 and 240 μg kg −1 for cadmium, 20, 94 and 120 μg kg −1 for lead, 1.1, 8.5 and 8.4 mg kg −1 for copper, 0.11, 1.3 and 1.3 mg kg −1 for manganese, 26, 50 and 30 mg kg −1 for zinc and 13, 54 and 63 mg kg −1 for iron, respectively. No definite time trends of mercury, cadmium and lead concentrations in swine muscle and edible Organ Meats during the period studied were observed, while for an essential trace metals analysed the values obtained seem to be typical. The results noted are reviewed and discussed with the data reported for muscle, liver and kidneys of swine in Poland from other studies.

Nicolette Gibson Hall - One of the best experts on this subject based on the ideXlab platform.

M.c. López-martínez - One of the best experts on this subject based on the ideXlab platform.

  • Copper in foods, beverages and waters from South East Spain: influencing factors and daily dietary intake by the Andalusian population.
    Food additives & contaminants. Part A Chemistry analysis control exposure & risk assessment, 2008
    Co-Authors: Carlos Velasco-reynold, Miguel Navarro-alarcón, H. Lopez-ga De La Serrana, M.c. López-martínez
    Abstract:

    The copper content of 225 food, 49 beverage and twelve potable water samples were determined using atomic absorption spectrometry (AAS). Analyses of NIST and BCR reference materials demonstrated the accuracy of this technique. The highest copper levels were found in dried fruit and legumes, followed by Organ Meats, molluscs and crustaceans, cephalopods, cereals and sausages, respectively. In cereals, legumes and fruit, copper levels increased significantly with increasing levels of protein and decreasing carbohydrate content (p 

  • Determination of Selenium in Meat Products by Hydride Generation Atomic Absorption SpectrometrySelenium Levels in Meat, Organ Meats, and Sausages in Spain
    Journal of Agricultural and Food Chemistry, 1996
    Co-Authors: Juana P. Diaz-alarcon, Miguel Navarro-alarcón, And Herminia López-garcía De La Serrana, M.c. López-martínez
    Abstract:

    The selenium contents, in samples of meat, Organ Meats, and sausages, have been determined using hydride generation atomic absorption spectrometry. Repeatability expressed as relative standard deviation ranged from 2.28% to 4.65%. Mean Se concentrations varied from 0.028 μg/g (fresh weight) for lamb to 1.196 μg/g (fresh weight) for pork kidney. The daily dietary intake of selenium supplied by this source is estimated to be 18.468 μg/person, on the basis of considering the average daily individual consumption of these foods in Andalusia (southern Spain) and their edible fraction. In the meat group, pork and chicken (4.689 and 2.726 μg/person, respectively) provide useful amounts of Se because of their high concentration and consumption in the daily diet. Keywords: Selenium in meat, Organ Meats, and sausages; daily dietary intake; HG-AAS

Ayako Yoshida - One of the best experts on this subject based on the ideXlab platform.

  • Development of nested multiplex polymerase chain reaction (PCR) assay for the detection of Toxocara canis, Toxocara cati and Ascaris suum contamination in meat and Organ Meats.
    Parasitology international, 2018
    Co-Authors: Zhenzhen Wang, Yen Thi Hoang Nguyen, Haruhiko Maruyama, Nariaki Nonaka, Miho Shibata, Yayoi Hayata, Ayako Yoshida
    Abstract:

    Abstract Ascarid Larva Migrans Syndrome (ascarid LMS) is a clinical syndrome in humans, caused by the migration of animal roundworm larvae such as Toxocara canis, Toxocara cati and Ascaris suum. Humans may acquire infection by ingesting embryonated eggs, or infective larvae of these parasites in contaminated meat and Organ Meats. To detect these pathogenic contaminations, a novel nested multiplex PCR system was developed. Our novel nested multiplex PCR assay showed specific amplification of T. canis, T. cati and Ascaris spp. Detection limit of the nested multiplex PCR was tested with serial dilution of T. canis, T. cati or A. suum genomic DNA (gDNA) from 100 pg to 100 ag and found to be 10 fg, 1 fg and 100 fg, respectively. When larvae were spiked into chicken liver tissue, DNA of T. canis and A. suum was detected from the liver spiked with a single larva, while the assay required at least 2 larvae of T. cati. Moreover, the ascarid DNA was detected from the liver of mice infected with 100 and 300 eggs of T. canis, T. cati or A. suum. This nested multiplex PCR assay could be useful for the detection of contamination with ascarid larvae in meat and Organ Meats.

  • evaluation of real time pcr assay for the detection of ascaris suum contamination in meat and Organ Meats
    Journal of Food Safety, 2017
    Co-Authors: Yen Thi Hoang Nguyen, Zhenzhen Wang, Haruhiko Maruyama, Yoichiro Horii, Nariaki Nonaka, Ayako Yoshida
    Abstract:

    Ascarid larva migrans syndrome (ascarid LMS) is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and Organ Meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and Organ Meats. Practical applications It has been considered that one of the most important risk factors for ascarid LMS in humans is the consumption of raw or undercooked meat or Organ Meats of domestic animals infected with Ascaris suum. Thus, we developed the novel real-time PCR with the high sensitivity and specificity in order to specifically detect A. suum DNA from animal tissues. This assay can be applied to the meat inspection procedure for the identification of parasite larval contamination that may adversely impact on public health as well as on animal health and welfare.

  • Evaluation of real‐time PCR assay for the detection of Ascaris suum contamination in meat and Organ Meats
    Journal of Food Safety, 2016
    Co-Authors: Yen Thi Hoang Nguyen, Zhenzhen Wang, Haruhiko Maruyama, Yoichiro Horii, Nariaki Nonaka, Ayako Yoshida
    Abstract:

    Ascarid larva migrans syndrome (ascarid LMS) is caused by ascarid roundworms including Ascaris suum. In East Asia, ascarid LMS has been considered as a food-borne disease in adulthood who has the dietary habitat of consuming raw or lightly cooked meat and Organ Meats. To evaluate the potential risk of A. suum infection from these foods, Ascaris specific real-time PCR was developed. The assay could constantly detect A. suum DNA up to 10 fg. Its specificity was confirmed by non-amplification with Toxocara canis and Toxocara cati DNA. A. suum DNA could be amplified from not only a single larva but also mouse liver spiked with a larva. Moreover, the assay could detect A. suum DNA in experimentally infected mouse liver, and showed higher sensitivity than a conventional digestion method. This real-time PCR assay would be useful for detecting the A. suum larval contamination in meat and Organ Meats. Practical applications It has been considered that one of the most important risk factors for ascarid LMS in humans is the consumption of raw or undercooked meat or Organ Meats of domestic animals infected with Ascaris suum. Thus, we developed the novel real-time PCR with the high sensitivity and specificity in order to specifically detect A. suum DNA from animal tissues. This assay can be applied to the meat inspection procedure for the identification of parasite larval contamination that may adversely impact on public health as well as on animal health and welfare.

Marina Bester - One of the best experts on this subject based on the ideXlab platform.