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Luiz Tadeu Moraes Figueiredo - One of the best experts on this subject based on the ideXlab platform.
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Oropouche Virus is detected in peripheral blood leukocytes from patients
Journal of Medical Virology, 2017Co-Authors: Luciano Kleber De Souza Luna, Rodrigo Ivo Marques Santos, Alcir Humberto Rodrigues, Maria Lucia Pereira Da Silva, Renata Sesticosta, Miria Ferreira Criado, Ronaldo B Martins, Luana Delcaro, Jose Luiz Proencamodena, Luiz Tadeu Moraes FigueiredoAbstract:Oropouche Virus (OROV) is a frequent cause of arboviral febrile disease in the Amazon. The present report describes studies done in two patients, one of them the first OROV human case acquired outside of the Amazon, which have revealed for the first time the presence of OROV in peripheral blood leukocytes. This novel finding raises important issues regarding pathogenesis of human infections and may offer a new tool, for the rapid diagnosis of this neglected infection. This article is protected by copyright. All rights reserved
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Clinical and virological descriptive study in the 2011 outbreak of dengue in the Amazonas, Brazil.
PLOS ONE, 2014Co-Authors: Valquiria Do Carmo Alves Martins, Felipe Gomes Naveca, Michele De Souza Bastos, João Bosco Lima Gimaque, Rajendranath Ramasawmy, Maurício Lacerda Nogueira, Wornei Silva Miranda Braga, Regina Pinto De Figueiredo, Sergio Nozawa, Luiz Tadeu Moraes FigueiredoAbstract:Background Dengue is a vector-borne disease in the tropical and subtropical region of the world and is transmitted by the mosquito Aedes aegypti. In the state of Amazonas, Brazil during the 2011 outbreak of dengue all the four Dengue Virus (DENV) serotypes circulating simultaneously were observed. The aim of the study was to describe the clinical epidemiology of dengue in Manaus, the capital city of the state of the Amazonas, where all the four DENV serotypes were co-circulating simultaneously. Methodology Patients with acute febrile illness during the 2011 outbreak of dengue, enrolled at the Fundacao de Medicina Tropical Dr. Heitor Viera Dourado (FMT-HVD), a referral centre for tropical and infectious diseases in Manaus, were invited to participate in a clinical and virological descriptive study. Sera from 677 patients were analyzed by RT-nested-PCRs for flaviViruses (DENV 1–4, Saint Louis encephalitis Virus-SLEV, Bussuquara Virus-BSQV and Ilheus Virus-ILHV), alphaVirus (Mayaro Virus-MAYV) and orthobunyaVirus (Oropouche Virus-OROV). Principal Findings Only dengue Viruses were detected in 260 patients (38.4%). Thirteen patients were co-infected with more than one DENV serotype and six (46.1%) of them had a more severe clinical presentation of the disease. Nucleotide sequencing showed that DENV-1 belonged to genotype V, DENV-2 to the Asian/American genotype, DENV-3 to genotype III and DENV-4 to genotype II. Conclusions Co-infection with more than one DENV serotype was observed. This finding should be warning signs to health authorities in situations of the large dispersal of serotypes that are occurring in the world.
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Detection of HerpesVirus, EnteroVirus, and ArboVirus infection in patients with suspected central nervous system viral infection in the Western Brazilian Amazon.
Journal of Medical Virology, 2014Co-Authors: Michele De Souza Bastos, Felipe Gomes Naveca, Luiz Tadeu Moraes Figueiredo, Rossicleia Lins Monte, Natália Lessa, Rajendranath Ramasawmy, Wornei Silva Miranda Braga, Maria Paula Gomes MourãoAbstract:Acute infections of the central nervous system (CNS) can be caused by various pathogens. In this study, the presence of herpesViruses (HHV), enteroViruses (EVs), and arboViruses were investigated in CSF samples from 165 patients with suspected CNS viral infection through polymerase chain reaction (PCR) and reverse transcriptase PCR. The genomes of one or more viral agents were detected in 29.7% (49/165) of the CSF samples. EVs were predominant (16/49; 32.6%) followed by Epstein-Barr Virus (EBV) (22.4%), Varicella-Zoster Virus (VZV) (20.4%), CytomegaloVirus (CMV) (18.4%), herpes simplex Virus (HSV-1) (4.1%), (HSV-2) (4.1%), and the arboViruses (14.3%). Four of the arboViruses were of dengue Virus (DENV) and three of Oropouche Virus (OROV). The detection of different Viruses in the CNS of patients with meningitis or encephalitis highlight the importance of maintaining an active laboratory monitoring diagnostics with rapid methodology of high sensitivity in areas of viral hyperendemicity that may assist in clinical decisions and in the choice of antiviral therapy. J. Med. Virol. 86:1522–1527, 2014. © 2014 Wiley Periodicals, Inc.
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Identification of Oropouche OrthobunyaVirus in the cerebrospinal fluid of three patients in the Amazonas, Brazil.
American Journal of Tropical Medicine and Hygiene, 2012Co-Authors: Michele De Souza Bastos, Felipe Gomes Naveca, Luiz Tadeu Moraes Figueiredo, Rossicleia Lins Monte, Natália Lessa, Regina Maria Pinto De Figueiredo, João Bosco Lima Gimaque, Guilherme Augusto Pivoto João, Rajendranath Ramasawmy, Maria Paula Gomes MourãoAbstract:Oropouche fever is the second most frequent arboviral infection in Brazil, surpassed only by dengue. Oropouche Virus (OROV) causes large and explosive outbreaks of acute febrile illness in cities and villages in the Amazon and Central-Plateau regions. Cerebrospinal fluid (CSF) samples from 110 meningoencephalitis patients were analyzed. The RNA extracted from fluid was submitted to reverse transcription-polymerase chain reaction and sequencing to identify OROV. Three CSF samples showed the presence of OROV causing infection in the central nervous system (CNS). These patients are adults. Two of the patients had other diseases affecting CNS and immune systems: neurocysticercosis and acquired immunodeficiency syndrome, respectively. Nucleotide sequence analysis showed that the OROV from the CSF of these patients belonged to genotype I. We show here that severe Oropouche disease is occurring during outbreaks of this Virus in Brazil
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Oropouche fever outbreak, Manaus, Brazil, 2007-2008.
Emerging Infectious Diseases, 2009Co-Authors: Maria Paula G. Mourão, Eurico Arruda, João Bosco Lima Gimaque, Michelle S. Bastos, Bruno Rafaelle Mota, Giselle S. Souza, Gustavo Henrique N. Grimmer, Elizabeth Dos Santos Galusso, Luiz Tadeu Moraes FigueiredoAbstract:To the Editor: Oropouche Virus (OROV) is an arboVirus, OrthobunyaVirus, transmitted among sloths, marsupials, primates, and birds by the mosquitoes Aedes serratus and Culex quinquefasciatus. Notably, this Virus has adapted to an urban cycle involving man, with midges (Culicoides paraensis) as the main vector (1). Oropouche fever is the second most frequent arboviral disease in Brazil, surpassed only by dengue. OROV causes large, explosive outbreaks of acute febrile illness in cities and villages in the Amazon and central regions of Brazil. An estimated 500,000 cases of OROV infection have occurred in Brazil in the past 48 years. In addition to outbreaks, OROV can also cause sporadic human infections (2). The Tropical Medicine Foundation of Amazonas State (TMF-AM) is a tertiary care center specializing in tropical and infectious diseases and is located in the city of Manaus. Syndromic surveillance for acute febrile illness has been conducted by TMF-AM since 1998. During January 2007 through November 2008, we obtained blood samples from 631 patients who had acute febrile illness for ≥5 days but who had negative results at initial screening for malaria (thick blood smear) and dengue (MAC-ELISA). Blood samples were tested for OROV immunoglobulin (Ig) M antibodies by an indirect enzyme immune assay using infected cells as antigen, as previously reported for dengue (3). For the indirect enzyme immune assay using infected cells as antigen, C6/36 A. albopictus cells were grown in 96 well microplates; these cells were infected with OROV (BeAn 1991 strain). After 4 days, the cells were fixed in the wells with 7% formalin buffered at pH 7.0. The microplate was blocked with 5% skim milk and, after washing the wells, 100 µL of serum diluted 1:400 was added into infected and uninfected wells. After incubation and washing the wells, a peroxidase-conjugated goat anti-human IgM was added; finally, the ABTS substrate (KPL, Inc., Gaithersburg, MD, USA) was added into the wells. The plates were incubated and read on a spectrophotometer at 405 nm. The cutoff for the test was determined to be the mean of optical densities read in all wells containing uninfected cells plus 3 standard deviations. Of the 631 patients in the study, 128 (20.3%) had IgM antibodies to OROV. The age range was 2–81 years (mean 29.5 ± 14 years), and 77 (60.2%) were women or girls. Most of the cases occurred November through March during the rainy season. In addition to fever, the patients had headache (93 [72.7%]), myalgia (90 [70.3%]), and arthralgia (74 [57.8%]). Rash was observed in 54 patients (42.2%), and hemorrhagic phenomena (petechiae, epistaxis, and gingival bleeding) were observed in 20 patients (15.5%). All patients recovered without sequelae and were not hospitalized. Despite the knowledge of the occurrence of several arboViruses in the Amazon region, most cases of arboviral diseases remain undiagnosed, probably because of their generally mild and self-limited clinical manifestations. Patients usually recover completely after a couple of days. However, even more severe cases may remain undiagnosed, especially because of long distances to health care facilities, difficulties in sample transportation, and lack of laboratory facilities capable of conducting the diagnostic assays. With regard to OROV infections, diagnosis of OROV may be easily confused with other acute febrile illness, including malaria and dengue, both of which are highly endemic in Manaus. In the present study, an inhouse enzyme immune assay for IgM using infected cell culture as antigen was found suitable for the diagnosis of OROV infections in the acute phase. Thus, a combination of a systematic surveillance for acute febrile illnesses and efficient laboratory diagnosis for OROV resulted in the discovery of an outbreak, which would probably have been overlooked if it had occurred in any region simultaneously with large dengue outbreaks or in the absence of laboratory diagnosis. The cases of OROV fever reported here likely represent a small portion of the cases; a much higher number of cases probably occurred in Manaus during the study period. The clinical characteristics of most cases of OROV fever in this outbreak were similar to previously reported descriptions of the illness. Notably, however, 20 (15.5%) patients from Manaus had spontaneous hemorrhagic phenomena (petecchiae, epistaxis, and gingival bleeding) that had not previously been described in OROV fever (4–6). Moreover, symptoms of involvement of the central nervous system were not observed. In recent years, the area of circulation and the epidemic potential of OROV have increased, and this Virus has emerged as a public health problem in Brazil and other countries in the Americas. Presently, OROV is the most common of the Brazilian zoonotic arboViruses infecting humans (7). Further evidence of the spread of OROV was its isolation in 2003 from a small primate, a marmoset (Callithrix), in the state of Minas Gerais in southeast Brazil, far from the Amazon region (8). Considering that midges (Culicoides paraensis) occur in most low altitude areas of the Americas, it is conceivable that environmental destruction and climate changes could result in OROV outbreaks in the large cities of Brazil, as well as in other parts of the Western Hemisphere (9).
Marcio Roberto Teixeira Nunes - One of the best experts on this subject based on the ideXlab platform.
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Oropouche fever: an overview of the epidemiological and molecular aspects in the brazilian Amazon region
2020Co-Authors: Febre Do, Marcio Roberto Teixeira Nunes, Jannifer Oliveira Chiang, Helena Baldez Vasconcelos, S. G. Rodrigues, Livia Caricio MartinsAbstract:The Oropouche Virus (OROV; Bunyaviridae, OrthobunyaVirus) is one of the most important arboVirus that infect humans in the Amazon region causing an arboviral disease denominated Oropouche fever. Between 1961 and 2006, several outbreaks were reported in different urban areas of Para (Belem, Santa Isabel, Castanhal, Santarem, Oriximina, Serra Pelada, zone Bragantina – Igarape Acu, Maracana e Magalhaes Barata), Amazonas (Manaus e Barcelos), Acre (Xapuri), Amapa (Mazagao), Maranhao (Porto Franco), Tocantins (Tocantinopolis) and Rondonia (Ariquemes e Oro Preto D’Oeste) states. Molecular studies recently conducted have demonstrated the circulation of at least three major OROV lineages in the Amazon region (genotypes I, II and III). The genotypes I and II are more frequently detected in the western and eastern Amazon, respectively. The genotype III, previously recognized only in Panama, was recently described in Southeast region of Brazil. The association of molecular and epidemiological data has contributed substantially for the genetic characterization of OROV strains isolated during different outbreaks in the past four decades, as well as providing a better understanding regarding its molecular epidemiology in terms of emergence of new lineages and dynamic of evolution of these arboViruses in the Americas mainly in the Amazon region. This work aims to present a comprehensive review regarding the epidemiological and molecular aspects of the OROV emphasizing its geographic distribution, dynamics of the outbreaks occurred between 1961 and 2006, as well as the genotype dispersion in Brazil.
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the evolutionary dynamics of Oropouche Virus orov in south america
bioRxiv, 2019Co-Authors: Bernardo Gutierrez, Emma L. Wise, Christopher H. Logue, Thomas A. Bowden, Steven T Pullan, Gabriel Trueba, Marcio Roberto Teixeira NunesAbstract:The Amazon basin is host to numerous arthropod-borne viral pathogens that cause febrile disease in humans. Among these, Oropouche orthobunyaVirus (OROV) is a relatively understudied member of the Peribunyavirales that causes periodic outbreaks in human populations in Brazil and other South American countries. Although several studies have described the genetic diversity of the Virus, the evolutionary processes that shape the viral genome remain poorly understood. Here we present a comprehensive study of the genomic dynamics of OROV that encompasses phylogenetic analysis, evolutionary rate estimates, inference of natural selective pressures, recombination and reassortment, and structural analysis of OROV variants. Our study includes all available published sequences, as well as a set of new OROV genomes sequences obtained from patients in Ecuador, representing the first set of viral genomes from this country. Our results show that differing evolutionary processes on the three segments that encompass the viral genome lead to variable evolutionary rates and TMRCAs that could be explained by cryptic reassortment. We also present the discovery of previously unobserved putative N-linked glycosylation sites, and codons which evolve under positive selection on the viral surface proteins, and discuss the potential role of these features in the evolution of the Virus through a combined phylogenetic and structural approach.
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Oropouche Virus clinical epidemiological and molecular aspects of a neglected orthobunyaVirus
American Journal of Tropical Medicine and Hygiene, 2017Co-Authors: Jorge Fernando Soares Travassos Da Rosa, Marcio Roberto Teixeira Nunes, Gustavo Olszanski Acrani, William Marciel De Souza, Francisco De Paula Pinheiro, Mario Luiz Figueiredo, Jedson Ferreira CardosoAbstract:Oropouche Virus (OROV) is an important cause of arboviral illness in Latin American countries, more specifically in the Amazon region of Brazil, Venezuela and Peru, as well as in other countries such as Panama. In the past decades, the clinical, epidemiological, pathological, and molecular aspects of OROV have been published and provide the basis for a better understanding of this important human pathogen. Here, we describe the milestones in a comprehensive review of OROV epidemiology, pathogenesis, and molecular biology, including a description of the first isolation of the Virus, the outbreaks during the past six decades, clinical aspects of OROV infection, diagnostic methods, genome and genetic traits, evolution, and viral dispersal.
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genetic analysis of members of the species Oropouche Virus and identification of a novel m segment sequence
Journal of General Virology, 2015Co-Authors: Sueli Guerreiro Rodrigues, Raimunda Do Socorro Da Silva Azevedo, Pedro Fernando Da Costa Vasconcelos, Gustavo Olszanski Acrani, Natasha L Tilstonlunel, Joseph Hughes, Daisy Elaine Andrade Da Silva, Marcio Roberto Teixeira NunesAbstract:Oropouche Virus (OROV) is a public health threat in South America, and in particular in northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil, in 2009. We also report the complete genome sequences of two OROV reassortants isolatd from two marmosets in Minas Gerais state, south-east Brazil, in 2012 that contained a novel M genome segment. Interestingly, all 10 isolates possessed a 947 nt S segment that lacked 11 residues in the S-segment 3′ UTR compared with the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated, and the identification of yet another reassortant highlights the importance of bunyaVirus surveillance in South America.
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establishment of a minigenome system for Oropouche Virus reveals the s genome segment to be significantly longer than reported previously
Journal of General Virology, 2015Co-Authors: Marcio Roberto Teixeira Nunes, Gustavo Olszanski Acrani, Natasha L Tilstonlunel, Daisy Elaine Andrade Da Silva, Martin Spiegel, Manfred Weidmann, Meik Dilcher, Richard M ElliottAbstract:Oropouche Virus (OROV) is a medically important orthobunyaVirus, which causes frequent outbreaks of a febrile illness in the northern parts of Brazil. However, despite being the cause of an estimated half a million human infections since its first isolation in Trinidad in 1955, details of the molecular biology of this tripartite, negative-sense RNA Virus remain limited. We have determined the complete nucleotide sequence of the Brazilian prototype strain of OROV, BeAn 19991, and found a number of differences compared with sequences in the database. Most notable were that the S segment contained an additional 204 nt at the 3′ end and that there was a critical nucleotide mismatch at position 9 within the base-paired terminal panhandle structure of each genome segment. In addition, we obtained the complete sequence of the Trinidadian prototype strain TRVL-9760 that showed similar characteristics to the BeAn 19991 strain. By using a T7 RNA polymerase-driven minigenome system, we demonstrated that cDNA clones of the BeAn 19991 L and S segments expressed functional proteins, and also that the newly determined terminal untranslated sequences acted as functional promoters in the minigenome assay. By co-transfecting a cDNA to the viral glycoproteins, Virus-like particles were generated that packaged a minigenome and were capable of infecting naive cells.
Pedro Fernando Da Costa Vasconcelos - One of the best experts on this subject based on the ideXlab platform.
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Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like Viruses
Memorias Do Instituto Oswaldo Cruz, 2017Co-Authors: Felipe Gomes Naveca, Valdinete Alves Do Nascimento, Victor Costa De Souza, Bruno Tardelli Diniz Nunes, Daniela Sueli Guerreiro Rodrigues, Pedro Fernando Da Costa VasconcelosAbstract:We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like Viruses carrying the Oropouche S segment, as well as the Mayaro Virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control. The specificity of the multiplex assay was evaluated by Primer-Blast analysis against the entire GenBank database, and further against a panel of 17 RNA arboViruses. The results indicated an accurate and highly sensitive assay with amplification efficiency greater than 98% for both targets, and a limit of detection between two and 20 copies per reaction. We believe that the assay described here will provide a tool for Mayaro and Oropouche Virus detection, especially in areas where differential diagnosis of Dengue, Zika and Chikungunya Viruses should be performed.
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PREVALÊNCIA DE ANTICORPOS CONTRA ARBOVÍRUS DA FAMÍLIA Bunyaviridae EM BÚFALOS DE ÁGUA
Ciência Animal Brasileira, 2015Co-Authors: Alexandre Do Rosário Casseb, Livia Caricio Martins, Jannifer Oliveira Chiang, Livia Medeiros Neves Casseb, Sandro Patroca Da Silva, Pedro Fernando Da Costa VasconcelosAbstract:The State of Para comprises 26% of Brazilian Amazon region where a large diversity of arboViruses has been described. This study sought to assess the prevalence and distribution of haemagglutination-inhibition antibodies against antigens of nine different types of arboVirus of the Bunyaviridae family, where eight were OrthobunyaVirus : Guaroa Virus , Maguari Virus , Tacaiuma Virus , Utinga Virus , Belem Virus , Caraparu Virus , Oropouche Virus and Catu Virus , and one PhleboVirus : Icoaraci Virus in sera samples of water buffaloes in Para State, Brazil. For all ArboViruses investigated there were antibodies, with the exception of Belem Virus . Antibodies to Maguari Virus were more prevalent (7.33%). The water buffaloes of the present study showed variable levels of antibodies in monotypic and heterotypic reactions that may indicate there are movements from most bunyaVirus studied in domestic buffaloes in the state of Para, and the Maguari Virus presents the largest circulation. Therefore, further studies are needed to investigate the role of water buffalo in the maintenance and dispersal of arboViruses, as well as whether these Viruses can cause disease in that species, especially in cases of birth defects and abortions. Keywords : Bubalus bubalis ; Bunyaviridae ; Haemagglutination Inhibition Test; Maguari Virus .
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genetic analysis of members of the species Oropouche Virus and identification of a novel m segment sequence
Journal of General Virology, 2015Co-Authors: Sueli Guerreiro Rodrigues, Raimunda Do Socorro Da Silva Azevedo, Pedro Fernando Da Costa Vasconcelos, Gustavo Olszanski Acrani, Natasha L Tilstonlunel, Joseph Hughes, Daisy Elaine Andrade Da Silva, Marcio Roberto Teixeira NunesAbstract:Oropouche Virus (OROV) is a public health threat in South America, and in particular in northern Brazil, causing frequent outbreaks of febrile illness. Using a combination of deep sequencing and Sanger sequencing approaches, we determined the complete genome sequences of eight clinical isolates that were obtained from patient sera during an Oropouche fever outbreak in Amapa state, northern Brazil, in 2009. We also report the complete genome sequences of two OROV reassortants isolatd from two marmosets in Minas Gerais state, south-east Brazil, in 2012 that contained a novel M genome segment. Interestingly, all 10 isolates possessed a 947 nt S segment that lacked 11 residues in the S-segment 3′ UTR compared with the recently redetermined Brazilian prototype OROV strain BeAn19991. OROV maybe circulating more widely in Brazil and in the non-human primate population than previously appreciated, and the identification of yet another reassortant highlights the importance of bunyaVirus surveillance in South America.
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Detection of arboViruses of public health interest in free-living New World primates (Sapajus spp.; Alouatta caraya) captured in Mato Grosso do Sul, Brazil
Revista Da Sociedade Brasileira De Medicina Tropical, 2013Co-Authors: Paulo Mira Batista, Sueli Guerreiro Rodrigues, Jannifer Oliveira Chiang, Renato Andreotti, Paulo Silva De Almeida, Alisson Marques, Pedro Fernando Da Costa VasconcelosAbstract:INTRODUCTION: A sero-epidemiological survey was undertaken to detect the circulation of arboViruses in free-living non-human primates. METHODS: Blood samples were obtained from 16 non-human primates (13 Sapajus spp. and three Alouatta caraya) that were captured using terrestrial traps and anesthetic darts in woodland regions in the municipalities of Campo Grande, Aquidauana, Jardim, Miranda and Corumba in the State of Mato Grosso do Sul, Brazil. The samples were sent to the Instituto Evandro Chagas (IEC) in Ananindeua, Para, Brazil, to detect antibodies against 19 species of arboViruses using a hemagglutination inhibition test (HI). RESULTS: Of the 16 primates investigated in the present study, five (31.2%) were serologically positive for an arboVirus. Of these five, two (12.5%) exhibited antibodies to the FlaviVirus genus, one (6.2%) exhibited a monotypic reaction to Cacipacore Virus, one (6.2%) was associated with Mayaro Virus, and one (6.2%) was positive for Oropouche Virus. CONCLUSIONS: Based on the positive serology observed in the present study, it was possible to conclude that arboViruses circulate among free-living primates. The Viruses in the areas studied might have been introduced by infected humans or by primates from endemic or enzootic areas. Studies of this nature, as well as efficient and continuous surveillance programs, are needed to monitor viral activities in endemic and enzootic regions.
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molecular epidemiology of Oropouche Virus brazil
Emerging Infectious Diseases, 2011Co-Authors: Helena Baldez Vasconcelos, Marcio Roberto Teixeira Nunes, Livia Medeiros Neves Casseb, Valeria Lima Carvalho, Eliana Vieira Pinto Da Silva, Mayra De Oliveira E Silva, Samir Mansour Moraes Casseb, Pedro Fernando Da Costa VasconcelosAbstract:Oropouche Virus (OROV) is the causative agent of Oropouche fever, an urban febrile arboviral disease widespread in South America, with >30 epidemics reported in Brazil and other Latin American countries during 1960– 2009. To describe the molecular epidemiology of OROV, we analyzed the entire N gene sequences (small RNA) of 66 strains and 35 partial Gn (medium RNA) and large RNA gene sequences. Distinct patterns of OROV strain clustered according to N, Gn, and large gene sequences, which suggests that each RNA segment had a different evolutionary history and that the classifi cation in genotypes must consider the genetic information for all genetic segments. Finally, time-scale analysis based on the N gene showed that OROV emerged in Brazil ≈223 years ago and that genotype I (based on N gene data) was responsible for the emergence of all other genotypes and for Virus dispersal.
Alan D T Barrett - One of the best experts on this subject based on the ideXlab platform.
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Jatobal Virus is a reassortant containing the small RNA of Oropouche Virus
Virus Research, 2001Co-Authors: Mohammad F Saeed, Robert B Tesh, Heiman Wang, Robert E Shope, Miguel T Suderman, David W C Beasley, Li Li, Amelia P. A. Travassos Da Rosa, Alan D T BarrettAbstract:Jatobal (JAT) Virus was isolated in 1985 from a carnivore (Nasua nasua) in Tucurui, Para state, Brazil and was classified as a distinct member of the Simbu serogroup of the BunyaVirus genus, family Bunyaviridae on the basis of neutralization tests. On the basis of nucleotide sequencing, we have found that the small (S) RNA of JAT Virus is very similar (>95% identity) to that of Oropouche (ORO) Virus, in particular, the Peruvian genotype of ORO Virus. In comparison, limited nucleotide sequencing of the G2 protein gene, encoded by the middle (M) RNA, of JAT and ORO Viruses, revealed relatively little identity (
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jatobal Virus is a reassortant containing the small rna of Oropouche Virus
Virus Research, 2001Co-Authors: Mohammad F Saeed, Amelia Travassos P A Da Rosa, Robert B Tesh, Heiman Wang, Robert E Shope, Miguel T Suderman, David W C Beasley, Li Li, Alan D T BarrettAbstract:Jatobal (JAT) Virus was isolated in 1985 from a carnivore (Nasua nasua) in Tucurui, Para state, Brazil and was classified as a distinct member of the Simbu serogroup of the BunyaVirus genus, family Bunyaviridae on the basis of neutralization tests. On the basis of nucleotide sequencing, we have found that the small (S) RNA of JAT Virus is very similar (>95% identity) to that of Oropouche (ORO) Virus, in particular, the Peruvian genotype of ORO Virus. In comparison, limited nucleotide sequencing of the G2 protein gene, encoded by the middle (M) RNA, of JAT and ORO Viruses, revealed relatively little identity (<66%) between these two Viruses. Neutralization tests confirmed the lack of cross-reactivity between the Viruses. These results suggest that JAT Virus is a reassortant containing the S RNA of ORO Virus. JAT Virus was attenuated in hamsters compared to ORO Virus suggesting that the S RNA of ORO Virus is not directly involved in hamster virulence.
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diagnosis of Oropouche Virus infection using a recombinant nucleocapsid protein based enzyme immunoassay
Journal of Clinical Microbiology, 2001Co-Authors: Mohammad F Saeed, Marcio Roberto Teixeira Nunes, Amelia Travassos P A Da Rosa, Pedro Fernando Da Costa Vasconcelos, Robert B Tesh, Douglas M Watts, Robert E Shope, Kevin L Russell, Alan D T BarrettAbstract:Oropouche (ORO) Virus is an emerging infectious agent that has caused numerous outbreaks of an acute febrile (dengue-like) illness among humans in Brazil, Peru, and Panama. Diagnosis of ORO Virus infection is based mainly on serology. Two different antigens, hamster serum antigen (HSA) and Vero cell lysate antigen (VCLA), are currently used in enzyme immunoassays (EIAs) in Brazil and Peru, respectively, to investigate the epidemiology of ORO Virus infection. Both antigens involve use of infectious Virus, and for this reason their use is restricted. Consequently, the frequency and distribution of ORO Virus infection are largely unexplored in other countries of South America. This report describes the use of a bacterially expressed recombinant nucleocapsid (rN) protein of ORO Virus in EIAs for the diagnosis of ORO Virus infection. The data revealed that the purified rN protein is comparable to the authentic viral N protein in its antigenic characteristics and is highly sensitive and specific in EIAs. Among 183 serum samples tested, a high degree of concordance was found between rN protein-based EIA and HSA- and VCLA-based EIAs for the detection of both ORO Virus-specific immunoglobulin M (IgM) and IgG antibodies. The high sensitivity, specificity, and safety of the rN protein-based EIA make it a useful diagnostic technique that can be widely used to detect ORO Virus infection in South America.
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nucleotide sequences and phylogeny of the nucleocapsid gene of Oropouche Virus
Journal of General Virology, 2000Co-Authors: Mohammad F Saeed, Marcio Roberto Teixeira Nunes, Pedro Fernando Da Costa Vasconcelos, Robert B Tesh, Scott C Weaver, Douglas M Watts, Heiman Wang, Robert E Shope, Alan D T BarrettAbstract:The nucleotide sequence of the S RNA segment of the Oropouche (ORO) Virus prototype strain TRVL 9760 was determined and found to be 754 nucleotides in length. In the virion-complementary orientation, the RNA contained two overlapping open reading frames of 693 and 273 nucleotides that were predicted to encode proteins of 231 and 91 amino acids, respectively. Subsequently, the nucleotide sequences of the nucleocapsid genes of 27 additional ORO Virus strains, representing a 42 year interval and a wide geographical range in South America, were determined. Phylogenetic analyses revealed that all the ORO Virus strains formed a monophyletic group that comprised three distinct lineages. Lineage I contained the prototype strain from Trinidad and most of the Brazilian strains, lineage II contained six Peruvian strains isolated between 1992 and 1998, and two strains from western Brazil isolated in 1991, while lineage III comprised four strains isolated in Panama during 1989.
Marcos Lazaro Moreli - One of the best experts on this subject based on the ideXlab platform.
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Identification of Simbu, California and Bunyamwera serogroup bunyaViruses by nested RT-PCR
Transactions of The Royal Society of Tropical Medicine and Hygiene, 2004Co-Authors: Marcos Lazaro Moreli, Victor Hugo Aquino, Luiz Tadeu Moraes FigueiredoAbstract:Abstract We describe a reverse transcription-polymerase chain reaction (RT-PCR) with primers that anneal to the 5′ and 3′ ends and amplify the BunyaVirus S RNA segments. The RT-PCR was done on the fluids of C6/36 cells infected with each of 21 bunyaViruses. The bunyaViruses studied, with the exception of Catu Virus, produced amplicons having 700 to 1300 base pairs and probably contained the whole S RNA segment sequence. A nested PCR performed with these amplicons distinguished California and most Bunyamwera serogroup Viruses from other bunyaViruses by use of BBC specific internal primers for the S RNA segment, and distinguished Simbu serogroup Viruses from others by use of BS specific internal primers. The nested-PCR amplicons of Guaroa, Maguari, California encephalitis, Bunyamwera, and Oropouche Viruses were sequenced. The sequences were aligned with previously known sequences of the S RNA segment of the same Viruses, showing a high degree of homology and thus confirming the specific origin of these amplicons. The nested RT-PCR is suitable as a specific screening for most California and Bunyamwera serogroup and Simbu serogroup Viruses depending on the use of BBC or BS internal primers. Oropouche Virus is an important public health problem in Brazil and the nested PCR with BS primers could be used for the detection of this Virus in tissue culture and mouse brain isolates as well as in clinical samples.
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diagnosis of Oropouche Virus infection by rt nested pcr
Journal of Medical Virology, 2002Co-Authors: Marcos Lazaro Moreli, Victor Hugo Aquino, Ana Cecilia Ribeiro Cruz, Luiz Tadeu Moraes FigueiredoAbstract:Using the RT-PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaViruses and internal primers that anneal to the S segment of Simbu serogroup Viruses in a nested PCR it was possible to amplify the Oropouche Virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley-Liss, Inc.
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Diagnosis of Oropouche Virus infection by RT‐nested‐PCR
Journal of Medical Virology, 2001Co-Authors: Marcos Lazaro Moreli, Victor Hugo Aquino, Ana Cecilia Ribeiro Cruz, Luiz Tadeu Moraes FigueiredoAbstract:Using the RT-PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaViruses and internal primers that anneal to the S segment of Simbu serogroup Viruses in a nested PCR it was possible to amplify the Oropouche Virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections. J. Med. Virol. 66:139–142, 2002. © 2002 Wiley-Liss, Inc.