The Experts below are selected from a list of 48 Experts worldwide ranked by ideXlab platform
Fuqiang Zhang - One of the best experts on this subject based on the ideXlab platform.
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Hepatitis Virus in Long-Fingered Bats, Myanmar
2016Co-Authors: Quanshui Fan, Fanli Yang, Ye Feng, Fuqiang Zhang, Wei Qiu, Huancheng GuoAbstract:During an analysis of the virome of bats from Myanmar, a large number of reads were annotated to orthohepadnavi-ruses. We present the full genome sequence and a morpho-logical analysis of an Orthohepadnavirus circulating in bats. This virus is substantially different from currently known members of the genus Orthohepadnavirus and represents a new species. The family Hepadnaviridae comprises 2 genera (Ortho-hepadnavirus and Avihepadnavirus), and viruses clas-sified within these genera have a narrow host range. The genus Orthohepadnavirus consists of pathogens that infect mammals, and it currently contains 4 species: hepatitis B virus, woodchuck hepatitis virus, ground squirrel hepati-tis virus, and woolly monkey hepatitis B virus. The genus Avihepadnavirus contains 2 avian species: duck hepatitis B virus and heron hepatitis B virus (1). Hepadnaviruses mainly infect the liver cells of their hosts and, in humans, cause hepatitis B, cirrhosis, and hepatocellular carcinoma (2). Approximately 2 billion persons worldwide are infect-ed with hepatitis B virus (HBV), and 600,000 persons die every year from the consequences of hepatitis B (3). Bats are associated with an increasing number of emerging and reemerging viruses, many of which pose major threats to public health (4). We conducted a viral metagenomic analysis of 6 species of bats from Myanmar. The analysis revealed a large number of viral contigs an-notated to Orthohepadnavirus with <70 % nt identity (B. He, unpub. data), suggesting the presence of orthohepad-naviruses in these animals. We describe the virus by full genomic analysis and morphologic observation
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identification of a novel Orthohepadnavirus in pomona roundleaf bats in china
Archives of Virology, 2015Co-Authors: Fuqiang Zhang, Ye Feng, Quanshui Fan, Wei Qiu, Lele Xia, Gang Chen, Huancheng GuoAbstract:Bats in Myanmar, Gabon, and Panama have been found to harbor diverse hepadnaviruses. Here, we report a novel hepadnavirus in 4 of 20 pomona roundleaf bats from Yunnan province, China. This virus contains 3,278 nucleotides (nt) in the full circularized genome, with four predicted open frames (ORFs) reading in the same direction. Full genomic sequence comparisons and evolutionary analysis indicate that this virus is a member of a new species within the genus Orthohepadnavirus
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Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses
PLOS ONE, 2013Co-Authors: Biao He, Zuosheng Li, Junfeng Zheng, Fanli Yang, Nan Su, Ye Feng, Yiyin Wang, Fuqiang ZhangAbstract:Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86% were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2%) contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45% (12,086/26,698) were related to vertebrate viruses, 28% (7,443/26,698) to insect viruses, 27% (7,074/26,698) to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats.
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Virome profiling of bats from myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses. PLoS One 8: e61950
2013Co-Authors: Fanli Yang, Junfeng Zheng, Ye Feng, Yiyin Wang, Huancheng Guo, Fuqiang ZhangAbstract:Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86 % were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2%) contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45 % (12,086/26,698) were related to vertebrate viruses, 28 % (7,443/26,698) to insect viruses, 27 % (7,074/26,698) to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats
Humberto J. Debat - One of the best experts on this subject based on the ideXlab platform.
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Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated with a concave-eared torrent frog (Odorrana tormota)
Archives of Virology, 2019Co-Authors: Humberto J. DebatAbstract:Viruses of the family Hepadnaviridae are characterized by partially dsDNA circular genomes of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses have a broad host range, which includes humans (hepatitis B virus), other mammals (genus Orthohepadnavirus ), and birds (genus Avihepadnavirus ). The known host specificity of hepadnaviruses has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. Tibetan frog hepatitis B virus (TFHBV) was recently discovered in a member of the species Nanorana parkeri (family Dicroglossidae) from Tibet. To increase our understanding of hepadnaviruses that infect amphibian hosts, we identified the full-length genome of a divergent strain, TFHBV-Ot, associated with a concave-eared torrent frog ( Odorrana tormota , family Ranidae) from China by searching deep-sequencing data. TFHBV-Ot shared a genomic organization and 76.6% overall genome sequence nucleotide identity with the prototype TFHBV associated with N. parkeri (TFHBV-Np). The pairwise amino acid sequence identity between the predicted gene products of TFHBV-Ot and TFHBV-Np ranged between 63.9% and 77.9%. Multiple tissue/organ-specific RNAseq datasets suggested a broad tropism of TFHBV, including muscle, gonads and brain. In addition, we provide information about putative virus-derived small RNAs from an amphibian hepadnavirus. The results presented here expand the known genetic diversity and host range of TFHBV to Ranidae frogs, and warrant an investigation of hepadnaviral infection of amphibian brains.
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Complete genome sequence of a divergent strain of Tibetan frog hepatitis B virus associated to concave-eared torrent frog Odorrana tormota
2018Co-Authors: Humberto J. DebatAbstract:The family Hepadnaviridae is characterized by partially dsDNA circular viruses of approximately 3.2 kb, which are reverse transcribed from RNA intermediates. Hepadnaviruses (HBVs) have a broad host range which includes humans (Hepatitis B virus), other mammals (genus Orthohepadnavirus), and birds (Avihepadnavirus). HBVs host specificity has been expanded by reports of new viruses infecting fish, amphibians, and reptiles. The tibetan frog hepatitis B virus (TFHBV) was recently discovered in Nanorana parkeri (Family Dicroglossidae) from Tibet. To increase understanding of hepadnavirus in amphibian host, we identified the full-length genome of a divergent strain TFHBV-Ot associated to the concave-eared torrent frog Odorrana tormota (Family Ranidae) from China by searching deep sequencing data. TFHBV-Ot shared the genomic organization and a 76.6% overall genome nucleotide identity to the prototype TFHBV associated to N. parkeri (TFHBV-Np). TFHBV-Ot amino acid pairwise identity with TFHBV-Np predicted gene products ranged between 63.9% and 77.9%. Multiple tissue/organ specific RNAseq datasets suggest a broad tropism of TFHBV including muscles, gonads and brains. In addition, we provide for the first time evidence of virus derived small RNA from an amphibian hepadnavirus, tentatively enriched in 19-20 nt species and cytidine as first base. The results presented here expand the genetic diversity and the host range of TFHBV to Ranidae frogs, and warrant investigation on hepadnaviral infection of amphibian brains.
Ye Feng - One of the best experts on this subject based on the ideXlab platform.
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Hepatitis Virus in Long-Fingered Bats, Myanmar
2016Co-Authors: Quanshui Fan, Fanli Yang, Ye Feng, Fuqiang Zhang, Wei Qiu, Huancheng GuoAbstract:During an analysis of the virome of bats from Myanmar, a large number of reads were annotated to orthohepadnavi-ruses. We present the full genome sequence and a morpho-logical analysis of an Orthohepadnavirus circulating in bats. This virus is substantially different from currently known members of the genus Orthohepadnavirus and represents a new species. The family Hepadnaviridae comprises 2 genera (Ortho-hepadnavirus and Avihepadnavirus), and viruses clas-sified within these genera have a narrow host range. The genus Orthohepadnavirus consists of pathogens that infect mammals, and it currently contains 4 species: hepatitis B virus, woodchuck hepatitis virus, ground squirrel hepati-tis virus, and woolly monkey hepatitis B virus. The genus Avihepadnavirus contains 2 avian species: duck hepatitis B virus and heron hepatitis B virus (1). Hepadnaviruses mainly infect the liver cells of their hosts and, in humans, cause hepatitis B, cirrhosis, and hepatocellular carcinoma (2). Approximately 2 billion persons worldwide are infect-ed with hepatitis B virus (HBV), and 600,000 persons die every year from the consequences of hepatitis B (3). Bats are associated with an increasing number of emerging and reemerging viruses, many of which pose major threats to public health (4). We conducted a viral metagenomic analysis of 6 species of bats from Myanmar. The analysis revealed a large number of viral contigs an-notated to Orthohepadnavirus with <70 % nt identity (B. He, unpub. data), suggesting the presence of orthohepad-naviruses in these animals. We describe the virus by full genomic analysis and morphologic observation
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identification of a novel Orthohepadnavirus in pomona roundleaf bats in china
Archives of Virology, 2015Co-Authors: Fuqiang Zhang, Ye Feng, Quanshui Fan, Wei Qiu, Lele Xia, Gang Chen, Huancheng GuoAbstract:Bats in Myanmar, Gabon, and Panama have been found to harbor diverse hepadnaviruses. Here, we report a novel hepadnavirus in 4 of 20 pomona roundleaf bats from Yunnan province, China. This virus contains 3,278 nucleotides (nt) in the full circularized genome, with four predicted open frames (ORFs) reading in the same direction. Full genomic sequence comparisons and evolutionary analysis indicate that this virus is a member of a new species within the genus Orthohepadnavirus
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Virome Profiling of Bats from Myanmar by Metagenomic Analysis of Tissue Samples Reveals More Novel Mammalian Viruses
PLOS ONE, 2013Co-Authors: Biao He, Zuosheng Li, Junfeng Zheng, Fanli Yang, Nan Su, Ye Feng, Yiyin Wang, Fuqiang ZhangAbstract:Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86% were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2%) contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45% (12,086/26,698) were related to vertebrate viruses, 28% (7,443/26,698) to insect viruses, 27% (7,074/26,698) to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats.
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Virome profiling of bats from myanmar by metagenomic analysis of tissue samples reveals more novel Mammalian viruses. PLoS One 8: e61950
2013Co-Authors: Fanli Yang, Junfeng Zheng, Ye Feng, Yiyin Wang, Huancheng Guo, Fuqiang ZhangAbstract:Bats are reservoir animals harboring many important pathogenic viruses and with the capability of transmitting these to humans and other animals. To establish an effective surveillance to monitor transboundary spread of bat viruses between Myanmar and China, complete organs from the thorax and abdomen from 853 bats of six species from two Myanmar counties close to Yunnan province, China, were collected and tested for their virome through metagenomics by Solexa sequencing and bioinformatic analysis. In total, 3,742,314 reads of 114 bases were generated, and over 86 % were assembled into 1,649,512 contigs with an average length of 114 bp, of which 26,698 (2%) contigs were recognizable viral sequences belonging to 24 viral families. Of the viral contigs 45 % (12,086/26,698) were related to vertebrate viruses, 28 % (7,443/26,698) to insect viruses, 27 % (7,074/26,698) to phages and 95 contigs to plant viruses. The metagenomic results were confirmed by PCR of selected viruses in all bat samples followed by phylogenetic analysis, which has led to the discovery of some novel bat viruses of the genera Mamastrovirus, Bocavirus, Circovirus, Iflavirus and Orthohepadnavirus and to their prevalence rates in two bat species. In conclusion, the present study aims to present the bat virome in Myanmar, and the results obtained further expand the spectrum of viruses harbored by bats
Andrea Rasche - One of the best experts on this subject based on the ideXlab platform.
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a novel hepatitis b virus species discovered in capuchin monkeys sheds new light on the evolution of primate hepadnaviruses
Journal of Hepatology, 2018Co-Authors: Breno Frederico De Carvalho Dominguez Souza, Alexander Konig, Andrea Rasche, Ianei De Oliveira Carneiro, Nora Stephan, Victor M Corman, Pia Luise RoppertAbstract:Background & Aims All known hepatitis B virus (HBV) genotypes occur in humans and hominoid Old World non-human primates (NHPs). The divergent woolly monkey HBV (WMHBV) forms another Orthohepadnavirus species. The evolutionary origins of HBV are unclear. Methods We analysed sera from 124 Brazilian monkeys collected during 2012–2016 for hepadnaviruses using molecular and serological tools, and conducted evolutionary analyses. Results We identified a novel Orthohepadnavirus species in capuchin monkeys (capuchin monkey hepatitis B virus [CMHBV]). We found CMHBV-specific antibodies in five animals and high CMHBV concentrations in one animal. Non-inflammatory, probably chronic infection was consistent with an intact preCore domain, low genetic variability, core deletions in deep sequencing, and no elevated liver enzymes. Cross-reactivity of antisera against surface antigens suggested antigenic relatedness of HBV, CMHBV, and WMHBV. Infection-determining CMHBV surface peptides bound to the human HBV receptor (human sodium taurocholate co-transporting polypeptide), but preferentially interacted with the capuchin monkey receptor homologue. CMHBV and WMHBV pseudotypes infected human hepatoma cells via the human sodium taurocholate co-transporting polypeptide, and were poorly neutralised by HBV vaccine-derived antibodies, suggesting that cross-species infections may be possible. Ancestral state reconstructions and sequence distance comparisons associated HBV with humans, whereas primate hepadnaviruses as a whole were projected to NHP ancestors. Co-phylogenetic analyses yielded evidence for co-speciation of hepadnaviruses and New World NHP. Bayesian hypothesis testing yielded strong support for an association of the HBV stem lineage with hominoid ancestors. Neither CMHBV nor WMHBV was likely the ancestor of the divergent human HBV genotypes F/H found in American natives. Conclusions Our data suggest ancestral co-speciation of hepadnaviruses and NHP, and an Old World origin of the divergent HBV genotypes F/H. The identification of a novel primate hepadnavirus offers new perspectives for urgently needed animal models of chronic hepatitis B. Lay summary The origins of HBV are unclear. The new Orthohepadnavirus species from Brazilian capuchin monkeys resembled HBV in elicited infection patterns and could infect human liver cells using the same receptor as HBV. Evolutionary analyses suggested that primate HBV-related viruses might have emerged in African ancestors of New World monkeys millions of years ago. HBV was associated with hominoid primates, including humans and apes, suggesting evolutionary origins of HBV before the formation of modern humans. HBV genotypes found in American natives were divergent from those found in American monkeys, and likely introduced along prehistoric human migration. Our results elucidate the evolutionary origins and dispersal of primate HBV, identify a new Orthohepadnavirus reservoir, and enable new perspectives for animal models of hepatitis B.
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bats carry pathogenic hepadnaviruses antigenically related to hepatitis b virus and capable of infecting human hepatocytes
Proceedings of the National Academy of Sciences of the United States of America, 2013Co-Authors: Jan Felix Drexler, Alexander Konig, Andrea Rasche, Victor M Corman, Andreas Geipel, Debby Van Riel, Lonneke M Leijten, Corinna M Bremer, Veronika M Cottontail, Gael Darren MagangaAbstract:The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative Orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV.
Victor M Corman - One of the best experts on this subject based on the ideXlab platform.
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a novel hepatitis b virus species discovered in capuchin monkeys sheds new light on the evolution of primate hepadnaviruses
Journal of Hepatology, 2018Co-Authors: Breno Frederico De Carvalho Dominguez Souza, Alexander Konig, Andrea Rasche, Ianei De Oliveira Carneiro, Nora Stephan, Victor M Corman, Pia Luise RoppertAbstract:Background & Aims All known hepatitis B virus (HBV) genotypes occur in humans and hominoid Old World non-human primates (NHPs). The divergent woolly monkey HBV (WMHBV) forms another Orthohepadnavirus species. The evolutionary origins of HBV are unclear. Methods We analysed sera from 124 Brazilian monkeys collected during 2012–2016 for hepadnaviruses using molecular and serological tools, and conducted evolutionary analyses. Results We identified a novel Orthohepadnavirus species in capuchin monkeys (capuchin monkey hepatitis B virus [CMHBV]). We found CMHBV-specific antibodies in five animals and high CMHBV concentrations in one animal. Non-inflammatory, probably chronic infection was consistent with an intact preCore domain, low genetic variability, core deletions in deep sequencing, and no elevated liver enzymes. Cross-reactivity of antisera against surface antigens suggested antigenic relatedness of HBV, CMHBV, and WMHBV. Infection-determining CMHBV surface peptides bound to the human HBV receptor (human sodium taurocholate co-transporting polypeptide), but preferentially interacted with the capuchin monkey receptor homologue. CMHBV and WMHBV pseudotypes infected human hepatoma cells via the human sodium taurocholate co-transporting polypeptide, and were poorly neutralised by HBV vaccine-derived antibodies, suggesting that cross-species infections may be possible. Ancestral state reconstructions and sequence distance comparisons associated HBV with humans, whereas primate hepadnaviruses as a whole were projected to NHP ancestors. Co-phylogenetic analyses yielded evidence for co-speciation of hepadnaviruses and New World NHP. Bayesian hypothesis testing yielded strong support for an association of the HBV stem lineage with hominoid ancestors. Neither CMHBV nor WMHBV was likely the ancestor of the divergent human HBV genotypes F/H found in American natives. Conclusions Our data suggest ancestral co-speciation of hepadnaviruses and NHP, and an Old World origin of the divergent HBV genotypes F/H. The identification of a novel primate hepadnavirus offers new perspectives for urgently needed animal models of chronic hepatitis B. Lay summary The origins of HBV are unclear. The new Orthohepadnavirus species from Brazilian capuchin monkeys resembled HBV in elicited infection patterns and could infect human liver cells using the same receptor as HBV. Evolutionary analyses suggested that primate HBV-related viruses might have emerged in African ancestors of New World monkeys millions of years ago. HBV was associated with hominoid primates, including humans and apes, suggesting evolutionary origins of HBV before the formation of modern humans. HBV genotypes found in American natives were divergent from those found in American monkeys, and likely introduced along prehistoric human migration. Our results elucidate the evolutionary origins and dispersal of primate HBV, identify a new Orthohepadnavirus reservoir, and enable new perspectives for animal models of hepatitis B.
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bats carry pathogenic hepadnaviruses antigenically related to hepatitis b virus and capable of infecting human hepatocytes
Proceedings of the National Academy of Sciences of the United States of America, 2013Co-Authors: Jan Felix Drexler, Alexander Konig, Andrea Rasche, Victor M Corman, Andreas Geipel, Debby Van Riel, Lonneke M Leijten, Corinna M Bremer, Veronika M Cottontail, Gael Darren MagangaAbstract:The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative Orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV.
