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Min Wang - One of the best experts on this subject based on the ideXlab platform.
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the comparison of biocompatibility and Osteoinductivity between multi walled and single walled carbon nanotube phbv composites
Journal of Materials Science: Materials in Medicine, 2018Co-Authors: Xun Xiao, Jinle Li, Qin Shan, Neel R. Nabar, Shibing Deng, Ye Tian, Min WangAbstract:The applications of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in tissue engineering have been widely studied. This study aimed to compare the biocompatibility and Osteoinductivity of single-walled carbon nanotubes (SWCNTs)/PHBV composites with multi-walled CNTs (MWCNTs)/PHBV composites. CNTs were dispersed in PHBV by ultrasonication and composites were created using thermal injection moulding. In order to test their biocompatibility and Osteoinductivity. Rat osteoblasts (rOBs) were then cultured and seeded on the composites. The composites were implanted in rat femoral bone defects. Our results showed that lower weight percentages of SWCNTs and MWCNTs (2–4%) improved both their mechanical and thermal decomposition properties. However, further reduction of rOBs cell death was observed in MWCNTs/PHBV. SWCNTs were shown to upregulate the expression of Runx-2 and Bmp-2 in early stage significantly, while MWCNTs showed a stronger long-term effect on Opn and Ocn. The in vivo result was that MWCNTs/PHBV composites induced intact rounding new bone, increased integration with new bone, and earlier completed bone remodeling when compared with SWCNTs. Immunohistochemistry also detected higher expression of RUNX-2 around MWCNTs/PHBV composites. In conclusion, there were no differences observed between SWCNTs and MWCNTs in the reinforcement of PHBV, while MWCNTs/PHBV composites showed better biocompatibility and Osteoinductivity both in vitro and in vivo.
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The comparison of biocompatibility and Osteoinductivity between multi-walled and single-walled carbon nanotube/PHBV composites
Journal of Materials Science: Materials in Medicine, 2018Co-Authors: Weiyi Pan, Jinle Li, Qin Shan, Xun Xiao, Yuan Yue, Neel R. Nabar, Shibing Deng, Ye Tian, Min Wang, Liang HaoAbstract:The applications of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in tissue engineering have been widely studied. This study aimed to compare the biocompatibility and Osteoinductivity of single-walled carbon nanotubes (SWCNTs)/PHBV composites with multi-walled CNTs (MWCNTs)/PHBV composites. CNTs were dispersed in PHBV by ultrasonication and composites were created using thermal injection moulding. In order to test their biocompatibility and Osteoinductivity. Rat osteoblasts (rOBs) were then cultured and seeded on the composites. The composites were implanted in rat femoral bone defects. Our results showed that lower weight percentages of SWCNTs and MWCNTs (2–4%) improved both their mechanical and thermal decomposition properties. However, further reduction of rOBs cell death was observed in MWCNTs/PHBV. SWCNTs were shown to upregulate the expression of Runx-2 and Bmp-2 in early stage significantly, while MWCNTs showed a stronger long-term effect on Opn and Ocn. The in vivo result was that MWCNTs/PHBV composites induced intact rounding new bone, increased integration with new bone, and earlier completed bone remodeling when compared with SWCNTs. Immunohistochemistry also detected higher expression of RUNX-2 around MWCNTs/PHBV composites. In conclusion, there were no differences observed between SWCNTs and MWCNTs in the reinforcement of PHBV, while MWCNTs/PHBV composites showed better biocompatibility and Osteoinductivity both in vitro and in vivo.
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Novel Electrospun Bicomponent Scaffolds for Bone Tissue Engineering: Fabrication, Characterization and Sustained Release of Growth Factor
MRS Proceedings, 2012Co-Authors: Chong Wang, Min Wang, Xiao-yan YuanAbstract:Electrospinning is a versatile technique for fabricating three-dimensional (3D) nanofibrous scaffolds and the scaffolds have been found to elicit desirable cellular behavior for tissue regeneration because the nanofibrous structures mimic the nanofibrous extracellular matrix (ECM) of biological tissues. From the material point of view, the ECM of bone is a nanofibrous nanocomposite consisting of an organic matrix (mainly collagen) and inorganic bone apatite nanoparticles. Therefore, for bone tissue engineering scaffolds, it is natural to construct nanofibrous nanocomposites having a biodegradable polymer matrix and nanosized bioactive bioceramics. Our previous studies demonstrated: (1) electrospun nanocomposite fiber loaded with calcium phosphate (Ca-P) were osteoconductive and could promote osteoblastic cell proliferation and differentiation better than pure polymer fibers; (2) The controlled release of recombinant human bone morphogenetic protein (rhBMP-2) from scaffolds provided the scaffolds with desired Osteoinductivity. In the current investigation, novel bicomponent scaffolds for bone tissue engineering were produced using our established dual-source dual-power electrospinning technique to achieve both osteoconductivity and Osteoinductivity. In the bicomponent scaffolds, one fibrous component was electrospun Ca-P/PLGA nanocomposite fibers and the other component was emulsion electrospun PDLLA nanofibers incorporated with rhBMP-2. Through electrospinning optimization, both fibers were evenly distributed in bicomponent scaffolds. The mass ratio of rhBMP-2/PDLLA fibers to Ca-P/PLGA fibers in bicomponent scaffolds could be controlled using multiple syringes. The structure and morphology of mono- and bicomponent scaffolds were examined. The in vitro release of rhBMP-2 from mono- and bicomponent scaffolds showed different release amount but similar release profile, exhibiting an initial burst release. Blending PDLLA with polyethylene glycol (PEG) could reduce the initial burst release of rhBMP-2. © 2012 Materials Research Society.
Xingdong Zhang - One of the best experts on this subject based on the ideXlab platform.
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correlations between macrophage polarization and osteoinduction of porous calcium phosphate ceramics
Acta Biomaterialia, 2020Co-Authors: Xuening Chen, Menglu Wang, Xiangfeng Li, Yumei Xiao, Fuying Chen, Jing Wang, Jie Liang, Xingdong ZhangAbstract:Abstract The host immune response is critical for in situ osteogenesis, but correlations between local inflammatory reactions and biomaterial osteoinduction are still poorly understood. This study used a murine intramuscular implantation model to demonstrate that calcium phosphate ceramics with different phase compositions exhibited divergent osteoinductivities. The osteoinductive potential of each ceramic was closely associated with the immunomodulatory capacity of the material, and especially with the regulation of macrophage polarization and functional status. Biphasic calcium phosphate (BCP) ceramics with superior osteoinductive potential enhanced the fraction of CD206+ M2 macrophages, up-regulated expression of M2 phenotypic markers in vitro, and increased the ARG+ M2 population in vivo. This suggested that BCP ceramics could ameliorate long-term inflammation and build a pro-osteogenic microenvironment. However, β-tricalcium phosphate (β-TCP) ceramics with no obvious Osteoinductivity increased the fraction of CCR7+ M1 macrophages, promoted the secretion of M1 phenotypic markers in vitro, and maintained a high proportion of iNOS+ M1 macrophages in vivo. It indicated that β-TCP ceramics could exacerbate inflammation and inhibit ectopic bone formation. Hydroxyapatite ceramics with an intermediate Osteoinductivity exhibited a moderate amount of both M1 and M2 macrophages. These findings highlight the critical role of macrophage polarization in biomaterial-dependent osteoinduction, which not only deepens our understanding of osteoinductive mechanisms but also provides a strategy to design bone substitutes by endowing materials with the proper immunomodulatory abilities to achieve the desired clinic performance. Statement of Significance Calcium phosphate (CaP) ceramics with osteoinductive capacities are able to induce ectopic bone formation in non-osseous sites. However, its underlying mechanism is largely unknown. Previous studies have demonstrated an indispensable role of macrophages in osteogenesis, inspiring us that local inflammatory reaction may affect material-dependent osteoinduction. This study indicated that CaP ceramics with different phase composition could present divergent osteoinductive capacities through modulating polarization and functional status of macrophages, as biphasic calcium phosphate with potent Osteoinductivity ameliorated long-term inflammation and induced a healing-associated M2 phenotype to initiate bone formation. These findings not only get an insight into the mechanism of CaP-involved osteoinduction, but also help the design of tissue-inducing implants by endowing biomaterials with proper immunomodulatory ability.
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enhanced Osteoinductivity of porous biphasic calcium phosphate ceramic beads with high content of strontium incorporated calcium deficient hydroxyapatite
Journal of Materials Chemistry B, 2018Co-Authors: Yanglong Deng, Xuening Chen, Menglu Wang, Xiangfeng Li, Yumei Xiao, Xingdong ZhangAbstract:Further biomimicking natural bone and enhancing Osteoinductivity to meet the requirements of regenerative medicine is the key development direction of biphasic calcium phosphate (BCP) ceramics. Bone mineral is a kind of Ca-deficient hydroxyapatite (CDHA) with many kinds of trace ions incorporated; however, little is actually known regarding the incorporation of trace ions in CDHA, and trace-ions-incorporated CDHA in BCP ceramics has seldom been studied. The present study introduces an effective approach to fabricate porous BCP ceramic beads with a high content of strontium (Sr)-incorporated CDHA (BCP-Sr), and investigated its biological performance, especially with regard to Osteoinductivity. The obtained BCP-Sr possessed a good spherical shape, interconnected pore structure, and a high content of Sr-incorporated CDHA phase. Compared to the commercial BCP ceramic irregular granules (BAM® P2040, BCP-C), BCP-Sr had more micropores, relatively faster degradation, better bone-like apatite formation, higher protein adsorption abilities, and was more likely to promote the related osteogenic genes and protein expressions of BMSCs. Further in vivo canine intramuscular implantation confirmed that BCP-Sr had higher Osteoinductivity than BCP-C. Collectively, the enhanced Osteoinductivity of porous BCP ceramic beads by introducing a high content of Sr-incorporated CDHA has significant implications for designing highly bioactive BCP ceramics for applying in regenerative medicine.
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The material and biological characteristics of osteoinductive calcium phosphate ceramics
Regenerative Biomaterials, 2017Co-Authors: Zhurong Tang, Xiangfeng Li, Xingdong ZhangAbstract:The discovery of Osteoinductivity of calcium phosphate (Ca-P) ceramics has set an enduring paradigm of conferring biological regenerative activity to materials with carefully designed structural characteristics. The unique phase composition and porous structural features of osteoinductive Ca-P ceramics allow it to interact with signaling molecules and extracellular matrices in the host system, creating a local environment conducive to new bone formation. Mounting evidence now indicate that the osteoinductive activity of Ca-P ceramics is linked to their physicochemical and three-dimensional structural properties. Inspired by this conceptual breakthrough, many laboratories have shown that other materials can be also enticed to join the rank of tissue-inducing biomaterials, and besides the bones, other tissues such as cartilage, nerves and blood vessels were also regenerated with the assistance of biomaterials. Here, we give a brief historical recount about the discovery of the Osteoinductivity of Ca-P ceramics, summarize the underlying material factors and biological characteristics, and discuss the mechanism of osteoinduction concerning protein adsorption, and the interaction with different types of cells, and the involvement of the vascular and immune systems.
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Surface structural biomimetics and the osteoinduction of calcium phosphate biomaterials.
Journal of Nanoscience and Nanotechnology, 2007Co-Authors: Toshiyuki Ikoma, Junzo Tanaka, Xingdong ZhangAbstract:From the point of view of materials, many natural tissues could be regarded as materials with certain nanostructure thus have special biological function. These natural nanostructures are generally formed under soft biological condition and show excellent biocompatibility and biological functions. To get materials with certain biological function, it is expectable to construct some nanostructure by mimicking the natural soft process. In this study, the porous biphasic calcium phosphate (BCP) ceramic was used to study the relationship of surface micro/nano structure and the biological function of Osteoinductivity. Surface structural biomimetic was achieved by a soft technology of surface treating with simulated body fluid (SBF) or SBF containing protein molecules (BSA-SBF). The biological function of osteoinduction was tested by studying the bone formation in the samples implanted in dog nonosseous sites. The results showed that by the soft biomimetic process, the surface was re-constructed and the surface characteristics varied with the biomimetic process. The biological function of Osteoinductivity depended on the surface characteristics. The present of protein molecules was likely to construct a surface structure which was different from that without the protein molecules and showed higher Osteoinductivity. The study gave the evidence of material surface structural biomimetic to promote biological function as well as the possibility of the application of soft-nano technology to construct a micro/nano structural surface/interface of biomaterials with certain biological function.
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surface characteristics and Osteoinductivity of biphasic calcium phosphate ceramics with different sintering temperature
Key Engineering Materials, 2006Co-Authors: Toshiyuki Ikoma, H L Wang, Ling Li Zhang, M Tanaka, Xingdong ZhangAbstract:Calcium phosphate (Ca-P) biomaterials have been proved to show Osteoinductivity, however the affecting factors and mechanism are still unclear now. In this study, the surface characteristics of biphasic Ca-P ceramics (hydroxyapatite/tricalcium phosphate; HA/TCP) sintered at the distinct temperature were investigated and the mechanism of the Osteoinductivity was discussed. The Osteoinductivity of HA/TCP ceramics increased with decreasing the sintering temperature. The different surface micro-structure resulted from different sintering temperature includes phase composition, surface micro-structure, and surface potential. These characteristics should be the important factors affecting Osteoinductivity.
G W Blunn - One of the best experts on this subject based on the ideXlab platform.
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intrinsic Osteoinductivity of porous titanium scaffold for bone tissue engineering
International Journal of Biomaterials, 2017Co-Authors: Maryam Tamaddon, Sorousheh Samizadeh, Ling Wang, G W BlunnAbstract:Large bone defects and nonunions are serious complications that are caused by extensive trauma or tumour. As traditional therapies fail to repair these critical-sized defects, tissue engineering scaffolds can be used to regenerate the damaged tissue. Highly porous titanium scaffolds, produced by selective laser sintering with mechanical properties in range of trabecular bone (compressive strength 35 MPa and modulus 73 MPa), can be used in these orthopaedic applications, if a stable mechanical fixation is provided. Hydroxyapatite coatings are generally considered essential and/or beneficial for bone formation; however, debonding of the coatings is one of the main concerns. We hypothesised that the titanium scaffolds have an intrinsic potential to induce bone formation without the need for a hydroxyapatite coating. In this paper, titanium scaffolds coated with hydroxyapatite using electrochemical method were fabricated and Osteoinductivity of coated and noncoated scaffolds was compared in vitro. Alizarin Red quantification confirmed osteogenesis independent of coating. Bone formation and ingrowth into the titanium scaffolds were evaluated in sheep stifle joints. The examinations after 3 months revealed 70% bone ingrowth into the scaffold confirming its osteoinductive capacity. It is shown that the developed titanium scaffold has an intrinsic capacity for bone formation and is a suitable scaffold for bone tissue engineering.
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osteoinduction of bone grafting materials for bone repair and regeneration
Bone, 2015Co-Authors: Elena Garciagareta, M J Coathup, G W BlunnAbstract:Abstract Regeneration of bone defects caused by trauma, infection, tumours or inherent genetic disorders is a clinical challenge that usually necessitates bone grafting materials. Autologous bone or autograft is still considered the clinical “gold standard” and the most effective method for bone regeneration. However, limited bone supply and donor site morbidity are the most important disadvantages of autografting. Improved biomaterials are needed to match the performance of autograft as this is still superior to that of synthetic bone grafts. Osteoinductive materials would be the perfect candidates for achieving this task. The aim of this article is to review the different groups of bone substitutes in terms of their most recently reported osteoinductive properties. The different factors influencing Osteoinductivity by biomaterials as well as the mechanisms behind this phenomenon are also presented, showing that it is very limited compared to Osteoinductivity shown by bone morphogenetic proteins (BMPs). Therefore, a new term to describe Osteoinductivity by biomaterials is proposed. Different strategies for adding Osteoinductivity (BMPs, stem cells) to bone substitutes are also discussed. The overall objective of this paper is to gather the current knowledge on Osteoinductivity of bone grafting materials for the effective development of new graft substitutes that enhance bone regeneration.
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the Osteoinductivity of silicate substituted calcium phosphate
Journal of Bone and Joint Surgery American Volume, 2011Co-Authors: M J Coathup, Sorousheh Samizadeh, Yvette S Fang, Thomas Buckland, Karin A Hing, G W BlunnAbstract:Background: The Osteoinductivity of silicate-substituted calcium phosphate and stoichiometric calcium phosphate was investigated with use of ectopic implantation. Implants with a macroporosity of 80% and a strut porosity of 30% were inserted into sites located in the left and right paraspinal muscles of six female sheep. Methods: After twelve weeks in vivo, a longitudinal thin section was prepared through the center of each implant. Bone formation within the implant, bone formation in contact with the implant surface, and implant resorption were quantified with use of a line intersection method. The specimens were also analyzed with use of backscattered scanning electron microscopy and energy-dispersive x-ray analysis. Results: Silicate substitution had a significant effect on the formation of bone both within the implant and on the implant surface during the twelve-week period. Bone area within the implant was greater in the silicate-substituted calcium phosphate group (mean, 7.65% ± 3.2%) than in the stoichiometric calcium phosphate group (0.99% ± 0.9%, p = 0.01). The amount of bone formed at the surface of the implant was also significantly greater in the silicate-substituted calcium phosphate group (mean, 26.00% ± 7.8%) than in the stoichiometric calcium phosphate group (2.2% ± 2.0%, p = 0.01). Scanning electron microscopy demonstrated bone formation within pores that were <5 mm in size, and energy-dispersive x-ray analysis confirmed the presence of silicon within the new bone in the silicate-substituted calcium phosphate group. Conclusions: The formation of bone within muscle during the twelve-week period showed both silicate-substituted calcium phosphate and stoichiometric calcium phosphate to be osteoinductive in an ovine model. Silicate substitution significantly increasedthe amount of bone that formed and the amount of bone attached to the implant surface. New bone formation occurred through an intramembranous process within the implant structure. Clinical Relevance: The use of a silicate-substituted calcium phosphate material instead of stoichiometric calcium phosphate ceramic during orthopaedic surgery may substantially augment repair and regeneration of bone.
Changqing Zhang - One of the best experts on this subject based on the ideXlab platform.
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strontium hydroxyapatite chitosan nanohybrid scaffolds with enhanced Osteoinductivity for bone tissue engineering
Materials Science and Engineering: C, 2017Co-Authors: Zhengliang Xu, Yi-xuan Chen, Qinfei Ke, Changqing ZhangAbstract:Abstract For the clinical application of bone tissue engineering with the combination of biomaterials and mesenchymal stem cells (MSCs), bone scaffolds should possess excellent biocompatibility and Osteoinductivity to accelerate the repair of bone defects. Herein, strontium hydroxyapatite [SrHAP, Ca10 − xSrx(PO4)6(OH)2]/chitosan (CS) nanohybrid scaffolds were fabricated by a freeze-drying method. The SrHAP nanocrystals with the different x values of 0, 1, 5 and 10 are abbreviated to HAP, Sr1HAP, Sr5HAP and Sr10HAP, respectively. With increasing x values from 0 to 10, the crystal cell volumes and axial lengths of SrHAP become gradually large because of the greater ion radius of Sr2 + than Ca2 +, while the crystal sizes of SrHAP decrease from 70.4 nm to 46.7 nm. The SrHAP/CS nanohybrid scaffolds exhibits three-dimensional (3D) interconnected macropores with pore sizes of 100–400 μm, and the SrHAP nanocrystals are uniformly dispersed within the scaffolds. In vitro cell experiments reveal that all the HAP/CS, Sr1HAP/CS, Sr5HAP/CS and Sr10HAP/CS nanohybrid scaffolds possess excellent cytocompatibility with the favorable adhesion, spreading and proliferation of human bone marrow mesenchymal stem cells (hBMSCs). The Sr5HAP nanocrystals in the scaffolds do not affect the adhesion, spreading of hBMSCs, but they contribute remarkably to cell proliferation and osteogenic differentiation. As compared with the HAP/CS nanohybrid scaffold, the released Sr2 + ions from the SrHAP/CS nanohybrid scaffolds enhance alkaline phosphatase (ALP) activity, extracellular matrix (ECM) mineralization and osteogenic-related COL-1 and ALP expression levels. Especially, the Sr5HAP/CS nanohybrid scaffolds exhibit the best Osteoinductivity among four groups because of the synergetic effect between Ca2 + and Sr2 + ions. Hence, the Sr5HAP/CS nanohybrid scaffolds with excellent cytocompatibility and osteogenic property have promising application for bone tissue engineering.
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enhanced antibacterial activity and Osteoinductivity of ag loaded strontium hydroxyapatite chitosan porous scaffolds for bone tissue engineering
Journal of Materials Chemistry B, 2016Co-Authors: Zhengliang Xu, Yi-xuan Chen, Qinfei Ke, Changqing ZhangAbstract:The properties of bone scaffolds, including biocompatibility, Osteoinductivity and antibacterial activity, are of great importance for reconstruction of large bone defects and prevention of implant-associated infections. Herein, we develop an Ag-loaded strontium hydroxyapatite (SrHAP)/chitosan (CS) porous scaffold (Ag–SrHAP/CS) according to the following steps: (i) freeze-drying fabrication of a SrHAP/CS porous scaffold; and (ii) deposition of Ag nanoparticles on the above scaffold. In addition, HAP/CS and Ag–HAP/CS porous scaffolds are prepared under the same conditions without doping Sr element. All the HAP/CS, Ag–HAP/CS, SrHAP/CS and Ag–SrHAP/CS porous scaffolds provide a friendly environment for the adhesion, spreading and proliferation of human bone marrow mesenchymal stem cells (hBMSCs). The three-dimensional (3D) interconnected macropores with a pore size of 100–400 μm allow the spreading of hBMSCs throughout the whole scaffolds. Interestingly, the Sr ions and Ag ions released from the Ag–SrHAP/CS porous scaffolds significantly enhance their Osteoinductivity and antibacterial activity, respectively. The Sr element in the SrHAP/CS and Ag–SrHAP/CS porous scaffolds increase the alkaline phosphatase (ALP) activity of hBMSCs, extracellular matrix (ECM) mineralization, and the expression levels of osteogenic-related genes BMP-2 and COL-I. Moreover, the Ag ions released from the Ag–HAP/CS and Ag–SrHAP/CS scaffolds can effectively inhibit the growth and attachment of Staphylococcus aureus (S. aureus, ATCC 25923). In conclusion, the Ag–SrHAP/CS porous scaffold possesses excellent biocompatibility, Osteoinductivity and antibacterial activity, so it has great potential for application in bone tissue engineering to repair bone defects and avoid infections.
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Enhanced antibacterial activity and Osteoinductivity of Ag-loaded strontium hydroxyapatite/chitosan porous scaffolds for bone tissue engineering
Journal of Materials Chemistry B, 2016Co-Authors: Zhengliang Xu, Yong Lei, Wenjing Yin, Yi-xuan Chen, Qinfei Ke, Ya-ping Guo, Changqing ZhangAbstract:The properties of bone scaffolds, including biocompatibility, Osteoinductivity and antibacterial activity, are of great importance for reconstruction of large bone defects and prevention of implant-associated infections. Herein, we develop an Ag-loaded strontium hydroxyapatite (SrHAP)/chitosan (CS) porous scaffold (Ag–SrHAP/CS) according to the following steps: (i) freeze-drying fabrication of a SrHAP/CS porous scaffold; and (ii) deposition of Ag nanoparticles on the above scaffold. In addition, HAP/CS and Ag–HAP/CS porous scaffolds are prepared under the same conditions without doping Sr element. All the HAP/CS, Ag–HAP/CS, SrHAP/CS and Ag–SrHAP/CS porous scaffolds provide a friendly environment for the adhesion, spreading and proliferation of human bone marrow mesenchymal stem cells (hBMSCs). The three-dimensional (3D) interconnected macropores with a pore size of 100–400 μm allow the spreading of hBMSCs throughout the whole scaffolds. Interestingly, the Sr ions and Ag ions released from the Ag–SrHAP/CS porous scaffolds significantly enhance their Osteoinductivity and antibacterial activity, respectively. The Sr element in the SrHAP/CS and Ag–SrHAP/CS porous scaffolds increase the alkaline phosphatase (ALP) activity of hBMSCs, extracellular matrix (ECM) mineralization, and the expression levels of osteogenic-related genes BMP-2 and COL-I. Moreover, the Ag ions released from the Ag–HAP/CS and Ag–SrHAP/CS scaffolds can effectively inhibit the growth and attachment of Staphylococcus aureus (S. aureus, ATCC 25923). In conclusion, the Ag–SrHAP/CS porous scaffold possesses excellent biocompatibility, Osteoinductivity and antibacterial activity, so it has great potential for application in bone tissue engineering to repair bone defects and avoid infections.
Xun Xiao - One of the best experts on this subject based on the ideXlab platform.
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The comparison of biocompatibility and Osteoinductivity between multi-walled and single-walled carbon nanotube/PHBV composites
Journal of Materials Science: Materials in Medicine, 2018Co-Authors: Weiyi Pan, Jinle Li, Qin Shan, Xun Xiao, Yuan Yue, Neel R. Nabar, Shibing Deng, Ye Tian, Min Wang, Liang HaoAbstract:The applications of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in tissue engineering have been widely studied. This study aimed to compare the biocompatibility and Osteoinductivity of single-walled carbon nanotubes (SWCNTs)/PHBV composites with multi-walled CNTs (MWCNTs)/PHBV composites. CNTs were dispersed in PHBV by ultrasonication and composites were created using thermal injection moulding. In order to test their biocompatibility and Osteoinductivity. Rat osteoblasts (rOBs) were then cultured and seeded on the composites. The composites were implanted in rat femoral bone defects. Our results showed that lower weight percentages of SWCNTs and MWCNTs (2–4%) improved both their mechanical and thermal decomposition properties. However, further reduction of rOBs cell death was observed in MWCNTs/PHBV. SWCNTs were shown to upregulate the expression of Runx-2 and Bmp-2 in early stage significantly, while MWCNTs showed a stronger long-term effect on Opn and Ocn. The in vivo result was that MWCNTs/PHBV composites induced intact rounding new bone, increased integration with new bone, and earlier completed bone remodeling when compared with SWCNTs. Immunohistochemistry also detected higher expression of RUNX-2 around MWCNTs/PHBV composites. In conclusion, there were no differences observed between SWCNTs and MWCNTs in the reinforcement of PHBV, while MWCNTs/PHBV composites showed better biocompatibility and Osteoinductivity both in vitro and in vivo.
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the comparison of biocompatibility and Osteoinductivity between multi walled and single walled carbon nanotube phbv composites
Journal of Materials Science: Materials in Medicine, 2018Co-Authors: Xun Xiao, Jinle Li, Qin Shan, Neel R. Nabar, Shibing Deng, Ye Tian, Min WangAbstract:The applications of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) in tissue engineering have been widely studied. This study aimed to compare the biocompatibility and Osteoinductivity of single-walled carbon nanotubes (SWCNTs)/PHBV composites with multi-walled CNTs (MWCNTs)/PHBV composites. CNTs were dispersed in PHBV by ultrasonication and composites were created using thermal injection moulding. In order to test their biocompatibility and Osteoinductivity. Rat osteoblasts (rOBs) were then cultured and seeded on the composites. The composites were implanted in rat femoral bone defects. Our results showed that lower weight percentages of SWCNTs and MWCNTs (2–4%) improved both their mechanical and thermal decomposition properties. However, further reduction of rOBs cell death was observed in MWCNTs/PHBV. SWCNTs were shown to upregulate the expression of Runx-2 and Bmp-2 in early stage significantly, while MWCNTs showed a stronger long-term effect on Opn and Ocn. The in vivo result was that MWCNTs/PHBV composites induced intact rounding new bone, increased integration with new bone, and earlier completed bone remodeling when compared with SWCNTs. Immunohistochemistry also detected higher expression of RUNX-2 around MWCNTs/PHBV composites. In conclusion, there were no differences observed between SWCNTs and MWCNTs in the reinforcement of PHBV, while MWCNTs/PHBV composites showed better biocompatibility and Osteoinductivity both in vitro and in vivo.
