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Serdar Sezer - One of the best experts on this subject based on the ideXlab platform.
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polypropylene composite hernia mesh with anti adhesion layer composed of polycaprolactone and Oxidized Regenerated Cellulose
Materials Science and Engineering: C, 2019Co-Authors: Umran Aydemir Sezer, Vildan Sanko, Ali Aktekin, Mehmet Gulmez, Gulderen Yanikkaya Demirel, Elif Sayman, Fugen Aker, Serdar SezerAbstract:Abstract Hernia surgeries are at the top of the general surgery operations. However, visceral adhesion, which is one of the worst complications of these operations, is still a major problem. One of the most preferred methods to prevent adhesion is the use of biomaterials. Polypropylene (PP) mesh is frequently preferred product in clinical applications owing to its mechanically robust structure against deformation within the body. However, PP meshes do not have anti-adhesive properties. Oxidized Regenerated Cellulose (ORC), on the other hand, is one of the most preferred products in preventing the adhesion in clinical use. ORC is not easily processable due to solubility limitations; and it must be used externally. In this study, for the first time, we designed a composite mesh structure with ORC and produced an antibacterial and anti-adhesive double-sided mesh by electro-spinning ORC micro-particles with poly(e‑caprolactone) (PCL) on PP mesh to form a composite structure. We conducted in vitro cell culture studies to determine bio-compatibility performances. We evaluated the anti-adhesion and comprehensive bio-compatibility studies through in vivo experiments. The results revealed that ORC presence and optimization of ORC degradation by coating with PCL play an important role in adhesion prevention and introduced a product prototype with efficient anti-adhesion properties.
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Oxidized Regenerated Cellulose cross linked gelatin microparticles for rapid and biocompatible hemostasis a versatile cross linking agent
Carbohydrate Polymers, 2018Co-Authors: Umran Aydemir Sezer, Isa Sahin, Zeynep Kocer, Gulderen Yanikkaya Demirel, Serdar SezerAbstract:Abstract Effective hemostatic materials are of utmost importance for preventing bleeding in emergencies and critical injuries. Combining biodegradability, good hemostatic properties and biocompatibility, gelatin is one of the most reliable materials clinically used for preventing internal bleeding in surgeries and for stopping external hemorrhage. Cross-linking is a useful method for enhancing the absorption capacity of gelatin and for controlling the degradation process. Existing and commonly used aldehyde-containing cross-linking agents lack reliability with respect to the control of hemostatic effect, solubility and toxicity. In this study; gelatin was cross-linked with sodium Oxidized Regenerated Cellulose (NaORC) to produce hemostatic microparticles. The NaORC was used at different ratios; and the studies on hemostatic efficiency and cytotoxicity under in vitro conditions demonstrated rapid arrest of bleeding alongside biocompatibility. These microparticles employing NaORC as a cross-linking agent for the first time demonstrated a unique structure for stopping bleeding with biocompatibility, and opened the way for different forms of cross-linked structures to be used in other biomaterials applications.
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Carboxymethyl Cellulose/Oxidized Regenerated Cellulose hydrogels as adhesion barriers: comparative study with different molecular weights and substitution degrees
Cellulose, 2016Co-Authors: Ali Aktekin, Umran Aydemir Sezer, Isa Sahin, Mehmet Gulmez, Selvinaz Ozkara, Serdar SezerAbstract:For hydrogel materials used in surgery to prevent post-operative adhesion formation, the ability to reduce adhesion formation effectively through ease of application is the most outstanding attribute for improving their clinical utility. In this study, hydrogel formulation with carboxymethyl Cellulose (CMC) and water soluble sodium Oxidized Regenerated Cellulose (ORC) powder was developed. The formulation was achieved with different molecular weights and degrees of substitution of the CMC to investigate the effects of these two variables on adhesion prevention. In vivo studies showed that hydrogel formulations with medium molecular weight and a higher degree of substitution gave the best anti-adhesion performance. Histological analyses indicated the materials did not damage the tissue at the surgery area. Promising results were obtained for the development of ORC containing hydrogel formulations for post-operative adhesion prevention applications.
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use of Oxidized Regenerated Cellulose as bactericidal filler for food packaging applications
Cellulose, 2016Co-Authors: Umran Aydemir Sezer, Vildan Sanko, Zehra Nur Yuksekdag, Dilek Uzundag, Serdar SezerAbstract:The packaging of food is one of the most essential parts of the food manufacturing process, since it provides sustainability for the quality and storage of food products. In this study, bactericidal materials to be used as food packaging applications were developed. Oxidized Regenerated Cellulose microparticles (ORCs) were synthesized and used as filler in a poly(e-caprolactone) (PCL) matrix for food packaging materials. In order to examine its usage in food packaging, the water uptake, water vapor and oxygen permeability were tested. Compared to pure PCL, addition of 4 % ORC decreased 93 and 70 % of the oxygen and water permeability, respectively. In vitro bactericidal activity was indicated against gram-negative Escherichia coli and gram-positive Staphylococcus aureus. Bactericidal activity against Listeria monocytogenes was also examined on salami to investigate the preservation and maintenance of food safety. Fifty percent of the total colony-forming units on salami was dead after 14 days of being packaged in ORC containing PCL composite material. Taken together, these results are promising for the development of ORC-containing active food packaging materials.
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development of metal ion binded Oxidized Regenerated Cellulose powder as hemostatic agent a comparative study with in vivo performance
Industrial & Engineering Chemistry Research, 2015Co-Authors: Zeynep Basagaoglu Demirekin, Umran Aydemir Sezer, Dilek Ulusoy Karatopuk, Serdar SezerAbstract:For hemostatic materials used in hemorrhage, the capacities to prevent blood loss and biofilm formation through bactericidal activity are the most important issues for improving their clinical utility. In this study, novel Oxidized Regenerated Cellulose (ORC) powder, which is a potassium salt form of ORC and sodium salt of ORC after NO2 mediated oxidation, was developed via a new and practical method. The materials indicated bactericidal effect against S. Aureus. Chemical characterizations indicated that Regenerated Cellulose was Oxidized selectively, and their crystalline structures are not affected by the oxidation treatment or synthesis procedure. In vivo hemostatic activity performances with rat injury models were studied. The in vivo studies showed that powder materials performed rapid blood coagulation. Histological analyses demonstrated the powder materials did not damage the tissue at the application area. Promising results were obtained for the development of biodegradable, bactericidal, and ease...
Hideo Ichimura - One of the best experts on this subject based on the ideXlab platform.
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Covering the staple line with polyglycolic acid sheet versus Oxidized Regenerated Cellulose mesh after thoracoscopic bullectomy for primary spontaneous pneumothorax
General Thoracic and Cardiovascular Surgery, 2018Co-Authors: Yuichiro Ozawa, Mitsuaki Sakai, Hideo IchimuraAbstract:Objective The aim of this study was to compare coverage with Oxidized Regenerated Cellulose mesh and that with polyglycolic acid sheet to decrease the incidence of postoperative recurrent pneumothorax. Methods From August 2010 to August 2014, a total of 112 patients with primary spontaneous pneumothorax undergoing thoracoscopic bullectomy were enrolled. We compared the clinicopathological characteristics between recurrent and non-recurrent cases and examined their association with the material used for visceral pleural coverage: polyglycolic acid sheet versus Oxidized Regenerated Cellulose mesh. Results 57 patients underwent thoracoscopic bullectomy plus coverage using Oxidized Regenerated Cellulose mesh and 55 underwent thoracoscopic bullectomy plus coverage using polyglycolic acid sheet. The recurrence rate among all patients was 13.3%. No severe postoperative complications were observed in either group. There were no significant differences in the perioperative outcomes. However, the postoperative recurrence rate was significantly higher in the Oxidized Regenerated Cellulose mesh group than in the polyglycolic acid sheet group (22.8 vs 3.6%). Conclusions Our results suggest that coverage with Oxidized regerated Cellulose mesh was not superior to coverage with polyglycolic acid sheet for postoperative recurrent pneumothorax.
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Covering the staple line with polyglycolic acid sheet versus Oxidized Regenerated Cellulose mesh after thoracoscopic bullectomy for primary spontaneous pneumothorax.
The Japanese Journal of Thoracic and Cardiovascular Surgery, 2018Co-Authors: Yuichiro Ozawa, Mitsuaki Sakai, Hideo IchimuraAbstract:Objective The aim of this study was to compare coverage with Oxidized Regenerated Cellulose mesh and that with polyglycolic acid sheet to decrease the incidence of postoperative recurrent pneumothorax.
Jin Mei He - One of the best experts on this subject based on the ideXlab platform.
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Hemostatic Effect of Aminated Multiwalled Carbon Nanotubes/Oxidized Regenerated Cellulose Nanocomposites.
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, A N AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC gauze is discussed.
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hemostatic effect of aminated multiwalled carbon nanotubes Oxidized Regenerated Cellulose nanocomposites
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, A N AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC gauze is discussed.
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manufacturing and physical characterization of absorbable Oxidized Regenerated Cellulose braided surgical sutures
International Journal of Biological Macromolecules, 2019Co-Authors: Feng Cheng, Hongbin Li, Carolina Chavezmadero, Ji Won Choi, Xiaotong Yi, Ting Zheng, Jin Mei HeAbstract:Abstract Suture is an important part of surgical operation, and closure of the wound associated with this procedure continuous to be a challenge in postoperative care. Currently, Oxidized Regenerated Cellulose (ORC) is widely used in the absorption of hemostatic materials. However, there is no ORC medical suture product in the market. The objective of this article was to prepare novel braided sutures by TEMPO-mediated oxidation Regenerated Cellulose (TORC) to achieve a suturable material with biodegradability and ideal mechanical properties. Regenerated Cellulose (RC) strands were made into sutures on a circular braiding machine, and TEMPO-mediated oxidation treatment was introduced alternatively after braiding. The RC sutures under different oxidation time were characterized by ATR-FTIR, electrical conductivity, XRD analysis, physical properties and in vitro degradation property. We further demonstrate that the RC sutures were Oxidized and formed the carboxylic (-COOH) functional group. With the extension of oxidation duration, the carboxyl content in TORC sutures increased gradually from 5.1 to 10.4% and the strength, weight, and diameter of TORC sutures decreased gradually. Moreover, we proved that the knot-pull strength of TORC-45 declined by 77.8% after 28 days, thus this sutures fulfilled U.S. Pharmacopeia requirement of knot-pull strength. We have shown that TEMPO oxidation reaction significantly promoted the degradation of TORC sutures. Overall, TORC sutures were successfully produced with favorable biodegradability, revealing potential prospects of clinical applications.
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Aminated multiwalled carbon nanotubes/Oxidized Regenerated Cellulose nanocomposites for haemostatic applications
Materials Today: Proceedings, 2018Co-Authors: Ali Nabipour Chakoli, Jin Mei He, Saeed Ahmadi, Yu Dong HuangAbstract:Abstract Commercial surgical absorbable hemostatic agents have been widely applied in various surgeries and played an important role on stopping the bleeding. The commercial Oxidized Regenerated Cellulose (ORC) has also shown several inherent disadvantages. To improve the hemostatic effect of ORC, it has been modified by incorporating aminated MWCNTs (MWCNT-NH2). For modification of neat ORC, the MWCNT-NH2s was reacted with neat ORC. The analytical results revealed a relatively strong interaction existed between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results revealed significant increment in water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composites. The haemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits showed that the aminated MWCNTs increased the rate of blood stopping, decreasing the blood loosing from injured sites.
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carbon nanotube modified Oxidized Regenerated Cellulose gauzes for hemostatic applications
Carbohydrate Polymers, 2018Co-Authors: Feng Cheng, Jin Mei He, Hongbin Li, Yunfeng Wang, Yunjia Song, Yu Dong HuangAbstract:Abstract Functionalized carbon nanotubes have recently received interest because of their unique properties, especially in the biomedical field. In this research, unmodified multiwalled carbon nanotubes (MWCNTs), and functionalized carbon nanotubes with amino groups (MWCNTs-NH2) and carboxyl groups (MWCNTs-COOH) were grafted to Oxidized Regenerated Cellulose (ORC) gauze to fabricate novel functionalized ORC, and the performance of the functionalized gauze was investigated. The functionalized ORC was characterized by FT-IR, XPS and SEM, which showed the different kinds of CNTs grafted on its surface. The XPS results demonstrated the successful incorporation of functionalized MWCNTs in the active layer of modified ORC gauze. Meanwhile, the specific surface area of the CNTs modified functionalized ORC gauze was improved in varying degrees, whereas the porosity was slightly decreased. Furthermore, hydrophilicity experiment results presented a significant increment in water uptake of the functionalized CNTs grafted to the surface of the ORC gauze. Results of the hemostatic performance test on rabbit ear artery and liver showed that compared with the original ORC gauze, the bleeding time was significantly reduced when using the functionalized CNTs modified ORC hemostatic gauze. Moreover, the results also showed that the MWCNTs-COOH/ORC functionalized gauze had outstanding hemostatic efficiency.
Michael P Diamond - One of the best experts on this subject based on the ideXlab platform.
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Effect of Oxidized Regenerated Cellulose (Interceed) on the expression of tissue plasminogen activator and plasminogen activator inhibitor-1 in human peritoneal fibroblasts and mesothelial cells.
Fertility and sterility, 2020Co-Authors: L April Gago, Ghassan M Saed, Eslam Elhammady, Michael P DiamondAbstract:To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to Oxidized Regenerated Cellulose (Interceed; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Control and Interceed-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). University research laboratory. Normal and adhesion fibroblasts and mesothelial cells. Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to Oxidized Regenerated Cellulose. Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA-PAI-1 ratio, an indicator of overall fibrinolytic activity. Interceed treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA-PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Interceed, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA-PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.
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effect of Oxidized Regenerated Cellulose interceed on the expression of tissue plasminogen activator and plasminogen activator inhibitor 1 in human peritoneal fibroblasts and mesothelial cells
Fertility and Sterility, 2006Co-Authors: April L Gago, Ghassan M Saed, Eslam Elhammady, Michael P DiamondAbstract:Objective To characterize the molecular changes that occur in normal fibroblasts, adhesion fibroblasts, and mesothelial cells as a result of exposure to Oxidized Regenerated Cellulose (Interceed®; Johnson & Johnson Medical, Inc., New Brunswick, NJ). Design Control and Interceed®-treated normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells in culture were assessed for messenger RNA levels of molecules known to be associated with adhesion development, using multiplex reverse transcriptase polymerase chain reaction (n = 4). Setting University research laboratory. Patient(s) Normal and adhesion fibroblasts and mesothelial cells. Intervention(s) Exposure of cells, normal fibroblasts, adhesion fibroblasts, and mesothelial cells to Oxidized Regenerated Cellulose. Main Outcome Measure(s) Real-time reverse transcriptase polymerase chain reaction expression of messenger RNA tissue plasminogen activator (tPA), plasminogen activator inhibitor-1 (PAI-1), and tPA–PAI-1 ratio, an indicator of overall fibrinolytic activity. Result(s) Interceed® treatment of normal peritoneal fibroblasts, adhesion fibroblasts, and mesothelial cells results in an increased expression of tPA in mesothelial cells and an increase in the tPA–PAI-1 ratio, signifying an overall increase in fibrinolytic activity. Conclusion(s) Interceed®, which has been shown in multiple human in vivo studies to decrease postoperative adhesion development, increases the expression of tPA and the tPA–PAI-1 ratio (an indicator of overall fibrinolytic activity), thereby promoting dissolution of fibrin and healing without adhesion development. Thus, the ability of Interceed® to reduce postoperative adhesion development may be derived from both a barrier and biologic effect.
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effects of Oxidized Regenerated Cellulose on the expression of extracellular matrix and transforming growth factor β1 in human peritoneal fibroblasts and mesothelial cells
American Journal of Obstetrics and Gynecology, 2003Co-Authors: April L Gago, Ghassan M Saed, Rona Wang, Michael Kruger, Michael P DiamondAbstract:Abstract Objective The purpose of this study was to evaluate the potential biologic effects of Oxidized Regenerated Cellulose, which has been shown in multiple human in vivo studies to reduce postoperative adhesion development, on the messenger RNA levels of transforming growth factor-β1, type I collagen, type III collagen, and fibronectin. Study design The Oxidized Regenerated Cellulose was dissolved in saline solution and added to confluent, monolayer cultures of human normal fibroblasts and mesothelial cells. Control cells were maintained in media alone at the same pH. After 24 hours of treatment, total RNA was extracted from all cells. Real-time reverse transcription–polymerase chain reaction was performed to determine the relative change in messenger RNA levels of type I, type HI collagen, fibronectin, transforming growth factor-β1, and β-actin (housekeeping gene) in response to the Oxidized Regenerated Cellulose treatment (n = 4 cultures). Student t tests were performed for each cell type, which compared Oxidized Regenerated Cellulose–treated cells to control cells. Calculated power for the statistically significant findings ranged from 65% to 100%. Results Transforming growth factor-β1 messenger RNA was elevated by the Oxidized Regenerated Cellulose treatment in the mesothelial cells by 13% (control cells, 0.562±0.022; Oxidized Regenerated Cellulose–treated cells, 0.636±0.014; P = .03). In normal fibroblasts, transforming growth factor-β1 messenger RNA was slightly, but not significantly, decreased in Oxidized Regenerated Cellulose–exposed normal fibroblasts compared with controls (control cells, 0.622±0.062; Oxidized Regenerated Cellulose–treated cells, 0.609±0.006; P = .85). Type I collagen was found to be increased by exposure to Oxidized Regenerated Cellulose in both mesothelial cells and normal peritoneal fibroblasts. Type I collagen was increased by 23% in mesothelial cells (control cells [0.587±0.018] vs Oxidized Regenerated Cellulose–treated cells [0.722±0.010], P = .002), and 27% in normal fibroblasts (control cells, 0.522±0.018, Oxidized Regenerated Cellulose–treated cells, 0.665±0.009; P = .001). However, messenger RNA levels of type III collagen and fibronectin (other representative molecules of the extracellular matrix) were not altered significantly by Oxidized Regenerated Cellulose application in vitro. Conclusion Oxidized Regenerated Cellulose influences the expression of factors that are accepted commonly to be associated with adhesiogenesis. Oxidized Regenerated Cellulose increased the expression of transforming growth factor-β1 in mesothelial cells and type I collagen in mesothelial cells and normal peritoneal fibroblasts. Minimization of these Oxidized Regenerated Cellulose–induced molecular changes, if they occur in vivo, may improve the ability of Oxidized Regenerated Cellulose to reduce postoperative adhesion development.
Yu Dong Huang - One of the best experts on this subject based on the ideXlab platform.
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Hemostatic Effect of Aminated Multiwalled Carbon Nanotubes/Oxidized Regenerated Cellulose Nanocomposites.
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, A N AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC gauze is discussed.
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hemostatic effect of aminated multiwalled carbon nanotubes Oxidized Regenerated Cellulose nanocomposites
Journal of Nanoscience and Nanotechnology, 2019Co-Authors: Baode Zhang, Jin Mei He, Yu Dong Huang, Ali Nabipour Chakoli, A N AleshinAbstract:: We have investigated the covalent conjugation of aminated multiwalled carbon nanotubes (MWCNTNH₂)s with Oxidized Regenerated Cellulose (ORC) in order to enhance the hemostatic effect. The MWCNT-NH₂s were prepared by functionalization of pristine MWCNTs (pMWCNTs) using amine groups. Neat ORC gauze and MWCNT-NH₂s were reacted using glutamic acid as cross linking bridge. We investigated an amination of pMWCNTs as well as the dispersion of MWCNT-NH₂s in the ORC gauze as matrix and their interfacial interactions by SEM and FT-IR. The results revealed that relatively strong interaction exists between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results in the significant increment of water uptake of MWCNT-NH₂s/ORC composites with increasing the concentration of MWCNT-NH₂s in composite. The in-vitro procoagulation test shows that the MWCNT-NH₂s/ORC gauzes have significant procoagulant activity. The hemostatic evaluation of MWCNT-NH₂s/ORC composites on rabbits shows that the aminated MWCNTs increase the rate of blood stopping and hence they decrease the blood loosing from injured sites. Hemostatic evaluation indicates that the MWCNT-NH₂s/ORC gauze has a valuable hemostatic performance. The products of platelets release reaction, activated platelets glycoprotein and activated clotting enzymes were increased simultaneously. The mechanism of the hemostasis for MWCNT-NH₂s/ORC gauze is discussed.
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Aminated multiwalled carbon nanotubes/Oxidized Regenerated Cellulose nanocomposites for haemostatic applications
Materials Today: Proceedings, 2018Co-Authors: Ali Nabipour Chakoli, Jin Mei He, Saeed Ahmadi, Yu Dong HuangAbstract:Abstract Commercial surgical absorbable hemostatic agents have been widely applied in various surgeries and played an important role on stopping the bleeding. The commercial Oxidized Regenerated Cellulose (ORC) has also shown several inherent disadvantages. To improve the hemostatic effect of ORC, it has been modified by incorporating aminated MWCNTs (MWCNT-NH2). For modification of neat ORC, the MWCNT-NH2s was reacted with neat ORC. The analytical results revealed a relatively strong interaction existed between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results revealed significant increment in water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composites. The haemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits showed that the aminated MWCNTs increased the rate of blood stopping, decreasing the blood loosing from injured sites.
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carbon nanotube modified Oxidized Regenerated Cellulose gauzes for hemostatic applications
Carbohydrate Polymers, 2018Co-Authors: Feng Cheng, Jin Mei He, Hongbin Li, Yunfeng Wang, Yunjia Song, Yu Dong HuangAbstract:Abstract Functionalized carbon nanotubes have recently received interest because of their unique properties, especially in the biomedical field. In this research, unmodified multiwalled carbon nanotubes (MWCNTs), and functionalized carbon nanotubes with amino groups (MWCNTs-NH2) and carboxyl groups (MWCNTs-COOH) were grafted to Oxidized Regenerated Cellulose (ORC) gauze to fabricate novel functionalized ORC, and the performance of the functionalized gauze was investigated. The functionalized ORC was characterized by FT-IR, XPS and SEM, which showed the different kinds of CNTs grafted on its surface. The XPS results demonstrated the successful incorporation of functionalized MWCNTs in the active layer of modified ORC gauze. Meanwhile, the specific surface area of the CNTs modified functionalized ORC gauze was improved in varying degrees, whereas the porosity was slightly decreased. Furthermore, hydrophilicity experiment results presented a significant increment in water uptake of the functionalized CNTs grafted to the surface of the ORC gauze. Results of the hemostatic performance test on rabbit ear artery and liver showed that compared with the original ORC gauze, the bleeding time was significantly reduced when using the functionalized CNTs modified ORC hemostatic gauze. Moreover, the results also showed that the MWCNTs-COOH/ORC functionalized gauze had outstanding hemostatic efficiency.
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aminated multiwalled carbon nanotubes Oxidized Regenerated Cellulose nanocomposites for haemostatic applications
Materials Today: Proceedings, 2018Co-Authors: Ali Nabipour Chakoli, Jin Mei He, Saeed Ahmadi, Yu Dong HuangAbstract:Abstract Commercial surgical absorbable hemostatic agents have been widely applied in various surgeries and played an important role on stopping the bleeding. The commercial Oxidized Regenerated Cellulose (ORC) has also shown several inherent disadvantages. To improve the hemostatic effect of ORC, it has been modified by incorporating aminated MWCNTs (MWCNT-NH2). For modification of neat ORC, the MWCNT-NH2s was reacted with neat ORC. The analytical results revealed a relatively strong interaction existed between aminated MWCNTs and the ORC macromolecules. The hydrophilicity test results revealed significant increment in water uptake of MWCNT-NH2s/ORC composites with increasing the concentration of MWCNT-NH2s in composites. The haemostatic evaluation of MWCNT-NH2s/ORC composites on rabbits showed that the aminated MWCNTs increased the rate of blood stopping, decreasing the blood loosing from injured sites.
