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Guan Ye - One of the best experts on this subject based on the ideXlab platform.
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a new phenolic glycoside from Paeonia lactiflora
Fitoterapia, 2006Co-Authors: Guan YeAbstract:A new phenolic glycoside , 2-methoxy-5-(E)-propenyl-phenol-β-vicianoside, was isolated from the root of Paeonia lactiflora. The structure of the new glycoside was elucidated by chemical and spectroscopic methods.
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A new phenolic glycoside from Paeonia lactiflora.
Fitoterapia, 2006Co-Authors: Guan YeAbstract:A new phenolic glycoside , 2-methoxy-5-(E)-propenyl-phenol-beta-vicianoside, was isolated from the root of Paeonia lactiflora. The structure of the new glycoside was elucidated by chemical and spectroscopic methods.
Daqiu Zhao - One of the best experts on this subject based on the ideXlab platform.
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Effects of drought stress on physiological responses and gene expression changes in herbaceous peony (Paeonia lactiflora Pall.).
Plant Signaling & Behavior, 2020Co-Authors: Tingting Li, Rong Wang, Daqiu ZhaoAbstract:Herbaceous peony (Paeonia lactiflora Pall.) is known as the flower phase. This phase is somewhat resistant to drought, but long-term drought and severe water shortage will affect its normal growth ...
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age associated methylation change of chi promoter in herbaceous peony Paeonia lactiflora pall
Bioscience Reports, 2018Co-Authors: Yanqing Wu, Daqiu ZhaoAbstract:Chalcone isomerase gene ( CHI ) is a key gene that regulates the formation of yellow traits in petals. To reveal transcriptional regulatory mechanisms of CHI gene in petals of Paeonia lactiflora, we investigated the CHI expression using qPCR, the pigment content by HPLC, and methylation levels using BSP+Miseq sequencing in 9Huangjinlun9 variety during different developmental stages including flower-bud stage (S1), initiating bloom (S2), bloom stage (S3), and withering stage (S4). Results showed that the expression level of CHI gene at S2 stage was significantly higher than that at other stages ( P < 0.05), and at S4 stage was extremely significantly lower than other stages ( P < 0.01). Besides, total anthocyanin, anthoxanthin and flavonoid contents in petals presented a similar trend with CHI expression during developmental stages. A total of 16 CpG sites varying methylation levels were detected in CHI gene core promoter region, of which the methylation levels at mC-4 and mC-16 sites were extremely significantly negatively correlated with CHI mRNA expression ( P < 0.01). mC-16 site is located in the binding region of C/EBPα transcription factor, suggesting that methylation at the mC-16 site may inhibit the binding of C/EBPα to CHI promoter DNA, thereby regulating the tissue-specific expression of CHI gene. Our study revealed the expression pattern of CHI gene in petal tissues of Paeonia lactiflora at different developmental stages, which is related to promoter methylation. Moreover, the important transcription regulation element-C/EBPα was identified, providing theoretical reference for in-depth study on the function of CHI gene in Paeonia lactiflora .
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Characterization of the Complete Chloroplast Genomes of Sequences of Two Diploid Species: Paeonia lactiflora ‘Da Fugui’ and Paeonia ostii ‘Fengdan’ in the Paeoniaceae Family
Journal of Horticulture, 2018Co-Authors: Ming Chen, Yujiang, Daqiu ZhaoAbstract:Paeonia ostii ‘Fengdan’ and Paeonia lactiflora ‘Da Fugui’ are two species of the same genus that are closely related. Paeonia ostii ‘Fengdan’ has a high nutritional and health value in woody oil crops, and is also a good parent material for intraspecific and interspecific hybridization; Paeonia lactiflora ‘Da Fugui’ is a kind of peony variety with high ornamental value. Both ‘Fengdan’ and ‘Da Fugui’are diploid species. The complete cp genome sizes of Paeonia ostii ‘Fengdan’ and Paeonia lactiflora ‘Da Fugui’ were 152731 and 157372bp, respectively. Paeonia ostii ‘Fengdan’ Small Single Copy Area (SSC) is 16968 bp, the Large Single Copy Area (LSC) is 84402 bp, the Inverse Repeat region (IR) is 25681 bp. Paeonia ostii ‘Fengdan’ encods a total of 151 genes, including 101 protein-coding genes, 43 tRNA genes and 7 rRNA genes. In addition, the Paeonia lactiflora ‘Da Fugui’ Small Single Copy Area (SSC) is 14822 bp, the Large Single Copy Area (LSC) is 82,043 bp, the Ieverse Repeat region (IR) is 30254 bp. Paeonia lactiflora ‘Da Fugui’ encods a total of 165 genes, including 110 protein-coding genes, 47 tRNA genes and 8 rRNA genes. The cluster analysis of the NJ(Neighbor-joining) tree showed that Paeoniae genus is distributed in a large branch, and is closely related to the other three genera. Paeonia ostii ‘Fengdan’ and Paeonia sp. Sd0052 are clustered together with herbaceous peony, while Paeonia lactiflora ‘Da Fugui’ is clustered more closely to peony.
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Comparative Analysis of Headspace Volatiles of Different Herbaceous Peony (Paeonia lactiflora Pall.) Cultivars
Journal of Essential Oil Bearing Plants, 2016Co-Authors: Liguo Feng, Yuemei Li, Lixia Sheng, Tinglin Li, Daqiu ZhaoAbstract:AbstractFlower fragrance not only plays an important role for reproduction of ornamental plants, but also improves the aesthetic value of cut flowers and potted plants, the quality of flower products and their economic value. In this study, the floral headspace compounds of different herbaceous peony cultivars were isolated by a headspace solid-phase microextraction, and followed by gas chromatography-mass spectrometry for identification and quantification. Up to 34 volatile compounds were identified from the 21 herbaceous peony cultivars, including 14 terpenes, 9 alcohols, 7 esters, 1 aldehydes, 1 ketones and 2 arenes. Terpenes, alcohols and esters were the major headspace compounds, whereas the main floral constituents identified in herbaceous peony were caryophyllene, β-citronellol and cinnamic acid methyl ester. Paeonia lactiflora ‘Zi Rong Xi Yao’, Paeonia lactiflora ‘Mo Zi Han Jin’, Paeonia lactiflora ‘Zi Yu Lian’ and Paeonia lactiflora ‘Xi Xia Ying Xue’ had a high total amount of β-citronellol, gera...
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an actin gene as the internal control for gene expression analysis in herbaceous peony Paeonia lactiflora pall
African Journal of Agricultural Research, 2012Co-Authors: Daqiu Zhao, Jintao GeAbstract:As an internal control gene, actin plays an important role in gene expression analysis. In this study, the full length cDNA and genomic DNA sequences of an actin gene were isolated from Chinese herbaceous peony (Paeonia lactiflora Pall.) for the first time. Sequence analysis indicated that the full length of cDNA was 1657 bp, containing one open reading frame (ORF) of 1134 bp that encodes 377 amino acids, and the genomic DNA sequence was 1817 bp, containing three exons and two introns. They all had been submitted to GenBank and obtained the accession number JN105299 and FJ713744, respectively. Homology analysis showed that the cDNA and its deduced protein of P. lactiflora actin (Plactin) shared high similarities with those from other plants. Protein structure prediction showed that the deduced protein did not have signal peptide and transmembrane topological structure, and was a hydrophilic and stable protein. Temporal and spatial expression analysis of Plactin, which was performed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), displayed its stable expression which conformed to the regulation of using as an internal control gene. The results suggested that actin of herbaceous peony could be used as an alternative internal control gene for the study of the targeted genes expression and regulation characteristics in herbaceous peony. Key words: Paeonia lactiflora, actin, internal control gene, gene expression.
Liu You-ping - One of the best experts on this subject based on the ideXlab platform.
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Determination and comparative study on contents of total phenols and tannin of Radix Paeoniae Rubra from Paeonia lactiflora Pall.and Paeonia veitchii Lynch.
Pharmacy and Clinics of Chinese Materia Medica, 2020Co-Authors: Liu You-pingAbstract:Objective:To determine and compare the contents of total phenols and tannin of Radix Paeoniae Rubra from Paeonia lactiflora Pall.and Paeonia veitchii Lynch.Method:The contents of total phenols and tannins in the 41 batches of Radix Paeoniae Rubra(including 12 batches Paeonia lactiflora Pall.and 29 batches Paeonia veitchii Lynch.) were determined by spectrophotometric scopic method with phosphomolybdium tungstic acid-casein reaction.And Mann-Whitney U test was used to analyze the results with software of SPSS18.0.Result:There was significant difference(P0.05) in the contents of tannin and total phenols between Paeonia lactiflora Pall.and Paeonia veitchii Lynch.The average concentration of total phenols were 57.60 mg·g-1and 96.42mg·g-1,tannin were 29.60 mg·g-1 and 59.73 mg·g-1 in Paeonia lactiflora Pall.and Paeonia veitchii Lynch.respectively.Conclusion:Significant difference is found in the contents of tannin and total phenols between Paeonia lactiflora Pall.and Paeonia veitchii Lynch.,and which in Paeonia veitchii Lynch.are more than Paeonia lactiflora Pall.
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Study on Seed Dormancy Breaking Method for Paeonia lactiflora
Liaoning Agricultural Sciences, 2020Co-Authors: Liu You-pingAbstract:Seeds of Paeonia lactiflora were used as test material.The seed dormancy breaking methods were studied by different treatments of rooting and germinating.The results indicated that the treatment of corrosion with concentrated H2SO4 for 1 min,then soaked at 300 mg / L GA3 for 24 h,and then with stratification in 15 ℃ reached the highest rooting rate,67.33%.seeds rooted after 27 days with lower mildew rate.The treatment of soaked at 300 mg / L GA3 for 24 h with temperature-accelerated stratification(4 ℃ for one month and then 15 ℃) could break the dormancy of hypocotyl after the length of root reaching 3 ～ 4 cm.Seeds germinated after 98 days and the rate of germination was 66.67%.
A M Pena - One of the best experts on this subject based on the ideXlab platform.
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the skin depigmenting potential of Paeonia lactiflora root extract and paeoniflorin in vitro evaluation using reconstructed pigmented human epidermis
International Journal of Cosmetic Science, 2016Co-Authors: M Chen, X Huang, J Chen, L Zhou, Peggy Sextius, A M Pena, S JeulinAbstract:Objective The roots of the herb Paeonia lactiflora (‘White Peony’) are used in association with other herbs in traditional clinical cosmetic practice in China as oral treatment for skin pigmentary disorders, such as brown or dark pigmentary spots. However, the skin-depigmenting potential of Paeonia lactiflora root extract and its main ingredient paeoniflorin has been scarcely investigated by topical application. The purpose of this study was to evaluate the efficacy of Paeonia lactiflora root extract and paeoniflorin as skin whitening agent in cosmetic application. Methods Paeonia lactiflora root extract (containing 53.25% of paeoniflorin) and paeoniflorin (97% purity) were applied topically on reconstructed pigmented human epidermis model, a three-dimensional (3D) human skin equivalent, showing morphological and functional characteristics similar to those of in vivo human skin. Two specific methods were used for quantifying melanin inside the reconstructed pigmented epidermis: Fontana–Masson staining (2D quantification) and multiphoton microscopy (3D quantification). Results Compared to vehicle (dimethyl sulfoxide DMSO), a significant decrease in 2D and 3D melanin content was observed after topical application on reconstructed pigmented epidermis of Paeonia lactiflora extract at 300 μg mL−1 (−28% and −27%, respectively) and paeoniflorin at 120 μg mL−1/250 μM (−30% and −23%, respectively), which is in the same order of magnitude as the positive reference 4-n-butylresorcinol at 83 μg mL−1/500 μM (−26% and −40%, respectively). Conclusion These results demonstrate, for the first time, the depigmenting potential of paeoniflorin and thus the potential interest of using Paeonia lactiflora root extracts containing paeoniflorin in cosmetic or dermatological applications for reducing the severity of some hyperpigmented skin disorders.
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The skin‐depigmenting potential of Paeonia lactiflora root extract and paeoniflorin: in vitro evaluation using reconstructed pigmented human epidermis
International Journal of Cosmetic Science, 2016Co-Authors: M Chen, X Huang, J Chen, L Zhou, Peggy Sextius, J. Ma, A M PenaAbstract:Objective The roots of the herb Paeonia lactiflora (‘White Peony’) are used in association with other herbs in traditional clinical cosmetic practice in China as oral treatment for skin pigmentary disorders, such as brown or dark pigmentary spots. However, the skin-depigmenting potential of Paeonia lactiflora root extract and its main ingredient paeoniflorin has been scarcely investigated by topical application. The purpose of this study was to evaluate the efficacy of Paeonia lactiflora root extract and paeoniflorin as skin whitening agent in cosmetic application. Methods Paeonia lactiflora root extract (containing 53.25% of paeoniflorin) and paeoniflorin (97% purity) were applied topically on reconstructed pigmented human epidermis model, a three-dimensional (3D) human skin equivalent, showing morphological and functional characteristics similar to those of in vivo human skin. Two specific methods were used for quantifying melanin inside the reconstructed pigmented epidermis: Fontana–Masson staining (2D quantification) and multiphoton microscopy (3D quantification). Results Compared to vehicle (dimethyl sulfoxide DMSO), a significant decrease in 2D and 3D melanin content was observed after topical application on reconstructed pigmented epidermis of Paeonia lactiflora extract at 300 μg mL−1 (−28% and −27%, respectively) and paeoniflorin at 120 μg mL−1/250 μM (−30% and −23%, respectively), which is in the same order of magnitude as the positive reference 4-n-butylresorcinol at 83 μg mL−1/500 μM (−26% and −40%, respectively). Conclusion These results demonstrate, for the first time, the depigmenting potential of paeoniflorin and thus the potential interest of using Paeonia lactiflora root extracts containing paeoniflorin in cosmetic or dermatological applications for reducing the severity of some hyperpigmented skin disorders.
M Chen - One of the best experts on this subject based on the ideXlab platform.
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the skin depigmenting potential of Paeonia lactiflora root extract and paeoniflorin in vitro evaluation using reconstructed pigmented human epidermis
International Journal of Cosmetic Science, 2016Co-Authors: M Chen, X Huang, J Chen, L Zhou, Peggy Sextius, A M Pena, S JeulinAbstract:Objective The roots of the herb Paeonia lactiflora (‘White Peony’) are used in association with other herbs in traditional clinical cosmetic practice in China as oral treatment for skin pigmentary disorders, such as brown or dark pigmentary spots. However, the skin-depigmenting potential of Paeonia lactiflora root extract and its main ingredient paeoniflorin has been scarcely investigated by topical application. The purpose of this study was to evaluate the efficacy of Paeonia lactiflora root extract and paeoniflorin as skin whitening agent in cosmetic application. Methods Paeonia lactiflora root extract (containing 53.25% of paeoniflorin) and paeoniflorin (97% purity) were applied topically on reconstructed pigmented human epidermis model, a three-dimensional (3D) human skin equivalent, showing morphological and functional characteristics similar to those of in vivo human skin. Two specific methods were used for quantifying melanin inside the reconstructed pigmented epidermis: Fontana–Masson staining (2D quantification) and multiphoton microscopy (3D quantification). Results Compared to vehicle (dimethyl sulfoxide DMSO), a significant decrease in 2D and 3D melanin content was observed after topical application on reconstructed pigmented epidermis of Paeonia lactiflora extract at 300 μg mL−1 (−28% and −27%, respectively) and paeoniflorin at 120 μg mL−1/250 μM (−30% and −23%, respectively), which is in the same order of magnitude as the positive reference 4-n-butylresorcinol at 83 μg mL−1/500 μM (−26% and −40%, respectively). Conclusion These results demonstrate, for the first time, the depigmenting potential of paeoniflorin and thus the potential interest of using Paeonia lactiflora root extracts containing paeoniflorin in cosmetic or dermatological applications for reducing the severity of some hyperpigmented skin disorders.
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The skin‐depigmenting potential of Paeonia lactiflora root extract and paeoniflorin: in vitro evaluation using reconstructed pigmented human epidermis
International Journal of Cosmetic Science, 2016Co-Authors: M Chen, X Huang, J Chen, L Zhou, Peggy Sextius, J. Ma, A M PenaAbstract:Objective The roots of the herb Paeonia lactiflora (‘White Peony’) are used in association with other herbs in traditional clinical cosmetic practice in China as oral treatment for skin pigmentary disorders, such as brown or dark pigmentary spots. However, the skin-depigmenting potential of Paeonia lactiflora root extract and its main ingredient paeoniflorin has been scarcely investigated by topical application. The purpose of this study was to evaluate the efficacy of Paeonia lactiflora root extract and paeoniflorin as skin whitening agent in cosmetic application. Methods Paeonia lactiflora root extract (containing 53.25% of paeoniflorin) and paeoniflorin (97% purity) were applied topically on reconstructed pigmented human epidermis model, a three-dimensional (3D) human skin equivalent, showing morphological and functional characteristics similar to those of in vivo human skin. Two specific methods were used for quantifying melanin inside the reconstructed pigmented epidermis: Fontana–Masson staining (2D quantification) and multiphoton microscopy (3D quantification). Results Compared to vehicle (dimethyl sulfoxide DMSO), a significant decrease in 2D and 3D melanin content was observed after topical application on reconstructed pigmented epidermis of Paeonia lactiflora extract at 300 μg mL−1 (−28% and −27%, respectively) and paeoniflorin at 120 μg mL−1/250 μM (−30% and −23%, respectively), which is in the same order of magnitude as the positive reference 4-n-butylresorcinol at 83 μg mL−1/500 μM (−26% and −40%, respectively). Conclusion These results demonstrate, for the first time, the depigmenting potential of paeoniflorin and thus the potential interest of using Paeonia lactiflora root extracts containing paeoniflorin in cosmetic or dermatological applications for reducing the severity of some hyperpigmented skin disorders.
