The Experts below are selected from a list of 1851 Experts worldwide ranked by ideXlab platform
Liang-sheng Wang - One of the best experts on this subject based on the ideXlab platform.
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Chemical Mechanism of Flower Color Microvariation in Paeonia with Yellow Flowers
Horticultural Plant Journal, 2020Co-Authors: Yong Yang, Cheng-yong Feng, Qianyu Wang, Liang-sheng WangAbstract:ABSTRACT Paeonia species are important ornamental plants, including three types: tree Peony, herbaceous Peony, and Itoh hybrid Peony. Paeonia with yellow color is desired and becoming popular. However, the chemical mechanism of the formation of yellow flowers in Paeonia is still unclear. In this study, 14 representative samples were selected: four samples of three species of Paeonia, four herbaceous Peony cultivars, three lutea hybrid tree Peony cultivars, and three Itoh hybrid Peony cultivars. The petal extracts of the samples were determined by HPLC-DAD and HPLC-Q-TOF-MS/MS. In total, 29 flavonoids were isolated and identified, including 28 flavonol glycosides and one chalcone derivative. There were significant differences in the composition and content of flavonoids in petals of different cultivars (species). The total content of flavonoids was between 19.430 and 143.043 mg • g−1 DW. Flavonol glycosides were detected in all samples. Chalconaringenin 2′-O-glucoside was detected from one herbaceous Peony cultivar ‘Golden Wheel’ lutea hybrid tree Peony cultivars, and Itoh Peony cultivars. The content of chalconaringenin 2′-O-glucoside in the samples was more than 43% of total flavonoids. The pigment type and content of lutea hybrid tree peonies and Itoh hybrid peonies are similar. Total flavonoids, especially quercetin 3-O-galloylglucoside and chalconaringenin 2′-O-glucoside contribute greatly to the formation of yellow flowers of Paeonia. The results of this study provide a comprehensive understanding of the chemical mechanism for yellow flower coloration of Paeonia, and lay the foundation for yellow flower breeding of Paeonia.
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a novel r2r3 myb transcription factor contributes to petal blotch formation by regulating organ specific expression of pschs in tree Peony paeonia suffruticosa
Plant and Cell Physiology, 2019Co-Authors: Jin Zhu, Qianyu Wang, Qingyan Shu, Qing Hao, Zhengan Liu, Yaowu Yuan, Yuanwen Duan, Siqi Men, Qinzheng Hou, Liang-sheng WangAbstract:Flower color patterns play critical roles in plant-pollinator interactions and represent one of the most common adaptations during angiosperm evolution. However, the molecular mechanisms underlying flower color pattern formation are less understood in non-model organisms. The aim of this study was to identify genes involved in the formation of petal blotches in tree Peony (Paeonia suffruticosa) through transcriptome profiling and functional experiments. We identified an R2R3-MYB gene, PsMYB12, representing a distinct R2R3-MYB subgroup, with a spatiotemporal expression pattern tightly associated with petal blotch development. We further demonstrated that PsMYB12 interacts with a basic helix-loop-helix (bHLH) and a WD40 protein in a regulatory complex that directly activates PsCHS expression, which is also specific to the petal blotches. Together, these findings advance our understanding of the molecular mechanisms of pigment pattern formation beyond model plants. They also benefit molecular breeding of tree Peony cultivars with novel color patterns and promote germplasm innovation.
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fatty acid desaturase 3 psfad3 from paeonia suffruticosa reveals high α linolenic acid accumulation
Plant Science, 2018Co-Authors: Dandan Yin, Cheng-yong Feng, Qingyan Shu, Liang-sheng WangAbstract:Abstract α-linolenic acid (ALA) deficiency and a skewed ω6: ω3 fatty acid ratio in the diet are thought to be a major cause for the high incidence of cardiovascular, inflammatory, and autoimmune diseases. Recent years, tree Peony (Paeonia suffruticosa Andr.) with the high proportion of ALA (more than 45% in seed oil) is widely concerned. However, the underlying accumulation mechanism of the ALA in tree Peony seeds remains unknown. In this study, comparative transcriptome analysis was performed between two cultivars (‘Saiguifei’ and ‘Jingshenhuanfa’) with different ALA contents. The analysis of the metabolic enzymes associated with ALA biosynthesis and temporal accumulation patterns of unsaturated fatty acids demonstrated the importance of microsomal ω-3 fatty acid desaturase 3 (FAD3). Moreover, PsFAD3 gene was identified from tree Peony seeds, which was located in endoplasmic reticulum and the expression levels of PsFAD3 were consistent with ALA accumulation patterns in seeds. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated PsFAD3 protein could catalyze ALA synthesis. These results indicated that PsFAD3 was involved in the synthesis of ALA in seeds and could be exploited by the genetic breeding of new cultivars with high ALA content in tree Peony as well as other potential crops.
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systematic qualitative and quantitative assessment of fatty acids in the seeds of 60 tree Peony paeonia section moutan dc cultivars by gc ms
Food Chemistry, 2015Co-Authors: Ruyu Yuan, Liang-sheng Wang, Liguang Chen, Xianghong Hao, Lijin Wang, Xuchen ZhengAbstract:Seeds from Paeonia ostii and Paeoniarockii have been recently identified as novel resources of α-linolenic acid (ALA) in China. To assess whether tree Peony cultivars can be used as oil resource, fatty acids (FAs) in 60 cultivars were monitored and evaluated in this study. The results indicated that the composition and content of FAs varied dramatically among different cultivars, in which ALA, linoleic acid, oleic acid, palmitic acid, and stearic acid were the dominant. The 60 cultivars were classified into six clusters by hierarchical cluster analysis, and they were quite distinct from each other. Finally, six cultivars with high yield and high quality were screened out, comprising of ‘Liuliguanzhu’, ‘Hongguanyupei’, ‘LSS-2’, ‘LSS-1’, ‘Jingshenhuanfa’ and ‘LSS-11’. These cultivars were appropriately applied in practical oil production. Overall, tree Peony oil with abundant unsaturated fatty acids especially ALA was proved to be a top-grade source for edible oil and nutritional supplements.
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fatty acid composition of developing tree Peony paeonia section moutan dc seeds and transcriptome analysis during seed development
BMC Genomics, 2015Co-Authors: Liang-sheng Wang, Qingyan Shu, Liguang Chen, Shuai Shao, Dandan YinAbstract:Tree Peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree Peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipid accumulation contents were measured using GC-MS methods across developing tree Peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. This study provides the first comprehensive genomic resources characterizing tree Peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.
Yanlong Zhang - One of the best experts on this subject based on the ideXlab platform.
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Virus-induced gene silencing in the perennial woody Paeonia ostii
PeerJ Inc., 2019Co-Authors: Lihang Xie, Lixin Niu, Qingyu Zhang, Daoyang Sun, Weizong Yang, Yanlong ZhangAbstract:Tree Peony is a perennial deciduous shrub with great ornamental and medicinal value. A limitation of its current functional genomic research is the lack of effective molecular genetic tools. Here, the first application of a Tobacco rattle virus (TRV)-based virus-induced gene silencing (VIGS) in the tree Peony species Paeonia ostii is presented. Two different approaches, leaf syringe-infiltration and seedling vacuum-infiltration, were utilized for Agrobacterium-mediated inoculation. The vacuum-infiltration was shown to result in a more complete Agrobacterium penetration than syringe-infiltration, and thereby determined as an appropriate inoculation method. The silencing of reporter gene PoPDS encoding phytoene desaturase was achieved in TRV-PoPDS-infected triennial tree Peony plantlets, with a typical photobleaching phenotype shown in uppermost newly-sprouted leaves. The endogenous PoPDS transcripts were remarkably down-regulated in VIGS photobleached leaves. Moreover, the green fluorescent protein (GFP) fluorescence was detected in leaves and roots of plants inoculated with TRV-GFP, suggesting the capability of TRV to silence genes in various tissues. Taken together, the data demonstrated that the TRV-based VIGS technique could be adapted for high-throughput functional characterization of genes in tree Peony
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Psycho-Physiological Effects of a Peony-Viewing Program on Middle-Aged and Elderly Individuals at Different Phenological Stages
MDPI AG, 2019Co-Authors: Ren-lin Zhao, Lixin Niu, Gang Zhang, Xi Wang, Bo-tong Zhang, Li-na Guo, Yanlong ZhangAbstract:To address the question of whether the behavior of humans to view different phenological Peony flowers has various effects on their physical and mental parameters, we investigated psycho-physiological responses of 74 participants (61.3 ± 10.78 years old) to environments of pre- and post-viewing tree peonies at four stages, including the exhibition leaf stage (ELS), initial bloom stage (IBS), full bloom stage (FBS), and terminal bloom stage (TBS). Physiological factors were examined using systolic blood pressure (SBP), diastolic blood pressure (DBP), heart rate (HR), fingertip pulse (FP), blood oxygen saturation (SpO2), and psychological evaluation, which was carried out using the Profile of Mood States (POMS) and State⁻Trait Anxiety Inventory (STAI). The results indicated that the SBP, DBP, HR, and FP levels of participants were significantly reduced after viewing tree peonies, whereas no remarkable alterations in SpO2 were found. The POMS scores of anger⁻hostility (A⁻H), fatigue⁻inertia (F⁻I), tension⁻anxiety (T⁻A), confusion⁻bewilderment (C⁻B), and depression⁻dejection (D⁻D) were significantly lower, but of vigor⁻activity (V⁻A) was higher post-viewing than pre-viewing. Furthermore, participants exhibited markedly decreased anxiety levels according to the STAI. Notably, the changes in measurement indices were more pronounced at the FBS. Our studies demonstrated that a short Peony-viewing program, especially at the FBS with completely opened and large tree Peony flowers, would be a promising therapeutic method for improving physiological functions as well as an effective psychological relaxation strategy for middle-aged and elderly individuals
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fatty acid and associated gene expression analyses of three tree Peony species reveal key genes for α linolenic acid synthesis in seeds
Frontiers in Plant Science, 2018Co-Authors: Qingyu Zhang, Lixin Niu, Lihang Xie, Mahbubur Rahman, Aruna Kilaru, Yanlong ZhangAbstract:The increasing demand for healthy edible oil has generated the need to identify promising oil crops. Tree Peony (Paeonia section Moutan DC.) is a woody oil crop with α-linolenic acid (ALA) contributing for 45% of the total fatty acid (FA) content in seeds. Molecular and genetic differences that contribute to varied FA content and composition among the wild Peony species are, however, poorly understood. Analyses of FA content and composition during seed development in three tree Peony species (Paeonia rockii, P. potaninii, and P. lutea) showed varied FA content among them with highest in P. rockii, followed by P. potaninii, and P. lutea. Total FA content among these species increased with seed development and reached its maximum in its final stage. Seed FA composition analysis of the three species also revealed that ALA (C18:3) was the most abundant, followed by oleic (C18:1) and linoleic (C18:2) acids. Additionally, quantitative real-time RT-PCR analyses of 10 key seed oil synthesis genes in the three tree Peony species revealed that FAD3, FAD2, β-PDHC, LPAAT, and Oleosin gene expression levels positively correlate with total FA content and rate of accumulation. Specifically, the abundance of FAD3 transcripts in P. rockii compared with P. potaninii, and P. lutea suggests that FAD3 might play an important role in synthesis of ALA via phosphatidylcholine-derived pathway. Overall, comparative analyses of FA content and composition in three different Peony species revealed a correlation between efficient lipid accumulation and lipid gene expression during seed development. Further characterization and metabolic engineering of these key genes from peonies will allow for subsequent improvement of tree Peony oil quality and production.
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effects of foliage boron spraying on seed yields and fatty acid composition of tree Peony paeonia ostii feng dan
Wuhan University Journal of Natural Sciences, 2018Co-Authors: Jiayi Sun, Jingxuan Yang, Shuangyu Wei, Yanlong ZhangAbstract:The influence of the foliage boron(B)-spaying concentration on the yield and fatty acid composition of tree Peony (Paeonia ostii ‘Feng Dan’) was explored in the experiment of 2014-2015. In this research, a statistically significant correlation was found between the mass concentration of foliage boron- spraying and tree Peony seed yield. Maximum yield increment of tree Peony seeds was obtained when the boron-spraying concentration was 4 g/L. The composition of fatty acid was analyzed by gas chromatography-mass spectrometry (GC-MS), and the main components of the fatty acid composition were quantified by internal standard method. The increase in the oil extraction rate after foliage boron-spraying nutrition of different concentration was significant and ranged between 0.04% and 11.43%. Seed oil production and oil extraction rate were also increased due to the increase of seed yields. Furthermore, foliage boron-spraying had a significant effect on the content of linolenic acid and linoleic acid in seed oil.
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determination of the phenolic content profile and antioxidant activity of seeds from nine tree Peony paeonia section moutan dc species native to china
Food Research International, 2017Co-Authors: Xiaoxiao Zhang, Qianqian Shi, Lixin Niu, Yanlong ZhangAbstract:As an important resource of functional food, the seeds of tree Peony are rich in phenolic compounds, which are associated with antioxidant activity. However, so far there has not been systematic study on phenolic compositions and antioxidant activity of the seeds from wild tree Peony species. The aim of this study was to determine the phenolic content, antioxidant compounds and antioxidant activity of seeds from nine tree Peony species native to China. Among the seed samples, Paeonia rockii had the highest total flavonoid content, strongest DPPH and ABTS radical scavenging activities, and strongest cupric reducing capacity; P. decomposita subsp. rotundiloba had the highest total phenolic and flavanol contents, as well as the strongest hydroxyl radical scavenging activity. Sixteen individual phenolic compounds were quantitatively measured, with (+)-catechin being the most abundant component. The content of the phenolic compounds luteolin, paeonol, and the total flavonoid content were significantly correlated with four antioxidant activities. Hierarchical cluster analysis showed that P. rockii and P. decomposita subsp. rotundiloba could be clustered in a group having a high phenolic content and strong antioxidant activity. These results suggest P. rockii and P. decomposita subsp. rotundiloba are the most promising candidates as useful sources of natural antioxidants.
Qingyan Shu - One of the best experts on this subject based on the ideXlab platform.
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a novel r2r3 myb transcription factor contributes to petal blotch formation by regulating organ specific expression of pschs in tree Peony paeonia suffruticosa
Plant and Cell Physiology, 2019Co-Authors: Jin Zhu, Qianyu Wang, Qingyan Shu, Qing Hao, Zhengan Liu, Yaowu Yuan, Yuanwen Duan, Siqi Men, Qinzheng Hou, Liang-sheng WangAbstract:Flower color patterns play critical roles in plant-pollinator interactions and represent one of the most common adaptations during angiosperm evolution. However, the molecular mechanisms underlying flower color pattern formation are less understood in non-model organisms. The aim of this study was to identify genes involved in the formation of petal blotches in tree Peony (Paeonia suffruticosa) through transcriptome profiling and functional experiments. We identified an R2R3-MYB gene, PsMYB12, representing a distinct R2R3-MYB subgroup, with a spatiotemporal expression pattern tightly associated with petal blotch development. We further demonstrated that PsMYB12 interacts with a basic helix-loop-helix (bHLH) and a WD40 protein in a regulatory complex that directly activates PsCHS expression, which is also specific to the petal blotches. Together, these findings advance our understanding of the molecular mechanisms of pigment pattern formation beyond model plants. They also benefit molecular breeding of tree Peony cultivars with novel color patterns and promote germplasm innovation.
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fatty acid desaturase 3 psfad3 from paeonia suffruticosa reveals high α linolenic acid accumulation
Plant Science, 2018Co-Authors: Dandan Yin, Cheng-yong Feng, Qingyan Shu, Liang-sheng WangAbstract:Abstract α-linolenic acid (ALA) deficiency and a skewed ω6: ω3 fatty acid ratio in the diet are thought to be a major cause for the high incidence of cardiovascular, inflammatory, and autoimmune diseases. Recent years, tree Peony (Paeonia suffruticosa Andr.) with the high proportion of ALA (more than 45% in seed oil) is widely concerned. However, the underlying accumulation mechanism of the ALA in tree Peony seeds remains unknown. In this study, comparative transcriptome analysis was performed between two cultivars (‘Saiguifei’ and ‘Jingshenhuanfa’) with different ALA contents. The analysis of the metabolic enzymes associated with ALA biosynthesis and temporal accumulation patterns of unsaturated fatty acids demonstrated the importance of microsomal ω-3 fatty acid desaturase 3 (FAD3). Moreover, PsFAD3 gene was identified from tree Peony seeds, which was located in endoplasmic reticulum and the expression levels of PsFAD3 were consistent with ALA accumulation patterns in seeds. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated PsFAD3 protein could catalyze ALA synthesis. These results indicated that PsFAD3 was involved in the synthesis of ALA in seeds and could be exploited by the genetic breeding of new cultivars with high ALA content in tree Peony as well as other potential crops.
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fatty acid composition of developing tree Peony paeonia section moutan dc seeds and transcriptome analysis during seed development
BMC Genomics, 2015Co-Authors: Liang-sheng Wang, Qingyan Shu, Liguang Chen, Shuai Shao, Dandan YinAbstract:Tree Peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree Peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipid accumulation contents were measured using GC-MS methods across developing tree Peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. This study provides the first comprehensive genomic resources characterizing tree Peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.
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two est derived marker systems for cultivar identification in tree Peony
Plant Cell Reports, 2012Co-Authors: Jian Zhang, Liang-sheng Wang, Qingyan Shu, Zhengan Liu, Hongxu Ren, E De KeyserAbstract:Tree Peony (Paeonia suffruticosa Andrews), a woody deciduous shrub, belongs to the section Moutan DC. in the genus of Paeonia of the Paeoniaceae family. To increase the efficiency of breeding, two EST-derived marker systems were developed based on a tree Peony expressed sequence tag (EST) database. Using target region amplification polymorphism (TRAP), 19 of 39 primer pairs showed good amplification for 56 accessions with amplicons ranging from 120 to 3,000 bp long, among which 99.3% were polymorphic. In contrast, 7 of 21 primer pairs demonstrated adequate amplification with clear bands for simple sequence repeats (SSRs) developed from ESTs, and a total of 33 alleles were found in 56 accessions. The similarity matrices generated by TRAP and EST-SSR markers were compared, and the Mantel test (r = 0.57778, P = 0.0020) showed a moderate correlation between the two types of molecular markers. TRAP markers were suitable for DNA fingerprinting and EST-SSR markers were more appropriate for discriminating synonyms (the same cultivars with different names due to limited information exchanged among different geographic areas). The two sets of EST-derived markers will be used further for genetic linkage map construction and quantitative trait locus detection in tree Peony.
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flavonoid composition and antioxidant activity of tree Peony paeonia section moutan yellow flowers
Journal of Agricultural and Food Chemistry, 2009Co-Authors: Liang-sheng Wang, Qingyan Shu, Yuanrun Zheng, Jingjing Zhang, Jie Zhang, Ruizhen YangAbstract:Tree Peony flowers are edible and traditional Chinese medicine materials. In the present study, 26 flavonoids were identified and quantified in yellow flowers of tree Peony by high-performance liquid chromatography with diode array detector (HPLC-DAD) and by HPLC−electrospray ionization−mass spectrometry (HPLC-ESI-MS). Seventeen of them were first reported in flowers of tree Peony, and glycosides of kaempferol, luteolin, and apigenin as well as isosalipurposide were the main flavonoids investigated. Furthermore, the petal extracts showed high antioxidant activity according to DPPH•, ABTS•+, and OH• scavenging assays and ferric reducing antioxidant power assay. There were significant correlations between antioxidant activity and both the total polyphenol content (determined by Folin−Ciocalteu method) and the total content of quercetin, kaempferol, and luteolin glycosides. This work is valuable for elucidation of phenolic composition in tree Peony flowers and for further utilization of them as functional fo...
Dandan Yin - One of the best experts on this subject based on the ideXlab platform.
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fatty acid desaturase 3 psfad3 from paeonia suffruticosa reveals high α linolenic acid accumulation
Plant Science, 2018Co-Authors: Dandan Yin, Cheng-yong Feng, Qingyan Shu, Liang-sheng WangAbstract:Abstract α-linolenic acid (ALA) deficiency and a skewed ω6: ω3 fatty acid ratio in the diet are thought to be a major cause for the high incidence of cardiovascular, inflammatory, and autoimmune diseases. Recent years, tree Peony (Paeonia suffruticosa Andr.) with the high proportion of ALA (more than 45% in seed oil) is widely concerned. However, the underlying accumulation mechanism of the ALA in tree Peony seeds remains unknown. In this study, comparative transcriptome analysis was performed between two cultivars (‘Saiguifei’ and ‘Jingshenhuanfa’) with different ALA contents. The analysis of the metabolic enzymes associated with ALA biosynthesis and temporal accumulation patterns of unsaturated fatty acids demonstrated the importance of microsomal ω-3 fatty acid desaturase 3 (FAD3). Moreover, PsFAD3 gene was identified from tree Peony seeds, which was located in endoplasmic reticulum and the expression levels of PsFAD3 were consistent with ALA accumulation patterns in seeds. Heterologous expression in Saccharomyces cerevisiae and Arabidopsis thaliana confirmed that the isolated PsFAD3 protein could catalyze ALA synthesis. These results indicated that PsFAD3 was involved in the synthesis of ALA in seeds and could be exploited by the genetic breeding of new cultivars with high ALA content in tree Peony as well as other potential crops.
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fatty acid composition of developing tree Peony paeonia section moutan dc seeds and transcriptome analysis during seed development
BMC Genomics, 2015Co-Authors: Liang-sheng Wang, Qingyan Shu, Liguang Chen, Shuai Shao, Dandan YinAbstract:Tree Peony (Paeonia section Moutan DC.) is known for its excellent ornamental and medicinal values. In 2011, seeds from P. ostii have been identified as novel resource of α-linolenic acid (ALA) for seed oil production and development in China. However, the molecular mechanism on biosynthesis of unsaturated fatty acids in tree Peony seeds remains unknown. Therefore, transcriptome data is needed to better understand the underlying mechanisms. In this study, lipid accumulation contents were measured using GC-MS methods across developing tree Peony seeds, which exhibited an extraordinary ALA content (49.3%) in P. ostii mature seeds. Transcriptome analysis was performed using Illumina sequencing platform. A total of 144 million 100-bp paired-end reads were generated from six libraries, which identified 175,874 contigs. In the KEGG Orthology enrichment of differentially expressed genes, lipid metabolism pathways were highly represented categories. Using this data we identified 388 unigenes that may be involved in de novo fatty acid and triacylglycerol biosynthesis. In particular, three unigenes (SAD, FAD2 and FAD8) encoding fatty acid desaturase with high expression levels in the fast oil accumulation stage compared with the initial stage of seed development were identified. This study provides the first comprehensive genomic resources characterizing tree Peony seeds gene expression at the transcriptional level. These data lay the foundation for further understanding of molecular mechanism responsible for lipid biosynthesis and the high unsaturated fatty acids (especially ALA) accumulation. Meanwhile, it provides theoretical base for potential oilseed application in the respect of n-6 to n-3 ratio for human diets and future regulation of target healthy components of oils.
Daqiu Zhao - One of the best experts on this subject based on the ideXlab platform.
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in vitro antioxidant and in vivo anti aging with stress resistance on caenorhabditis elegans of herbaceous Peony stamen tea
International Journal of Food Properties, 2021Co-Authors: Daqiu Zhao, Jiasong Meng, Menglin Cheng, Lei Liu, Jing Sun, Jorge Alberto Condoriapfata, Jun TaoAbstract:Herbaceous Peony (Paeonia lactiflora Pall.) is a traditional Chinese medicinal herb with roots; however, the flowers, specifically the stamens have not been exploited. In this study, the main activ...
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melatonin and expression of tryptophan decarboxylase gene tdc in herbaceous Peony paeonia lactiflora pall flowers
Molecules, 2018Co-Authors: Daqiu Zhao, Rong Wang, Yanqing WuAbstract:Melatonin is a bioactive, edible ingredient that promotes human health and exists widely in plants, but little is known about its biosynthetic routes and underlying molecular mechanisms in the herbaceous Peony. In this contribution, we found that herbaceous Peony flowers are rich in melatonin that is found in the greatest quantities in the white series, followed by the ink series, the red series and then the pink series. On this basis, the melatonin content fluctuates during flower development and peaks during the bloom stage. Moreover, it is apparent that sun exposure and blue light induce melatonin production whereas green light restrains it during a 24-h light/dark cycle of melatonin content, as there were ‘dual peaks’ at 2 p.m. and 2 a.m. Additionally, the corresponding expression pattern of the herbaceous Peony tryptophan decarboxylase gene (TDC) was positively related with melatonin production. These results suggest that color series, development stage and light play an important role in melatonin accumulation, and that TDC is a rate-limiting gene in melatonin biosynthesis.
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daminozide reduces red color intensity in herbaceous Peony paeonia lactiflora pall flowers by inhibiting the expression of flavonoid biosynthetic genes
3 Biotech, 2018Co-Authors: Yuhan Tang, Daqiu ZhaoAbstract:Daminozide is a plant growth retardant that inhibits 2-oxoglutarate-dependent dioxygenase activity in the gibberellin biosynthesis pathway, but few studies have focused on its effect on the flavonoid biosynthesis pathway involved in flower color. In the present study, the effect of daminozide on the regulation of flower color was investigated. An herbaceous Peony (Paeonia lactiflora Pall.) cultivar ‘Fenzhu Pan’ was used as the material and treated with daminozide. Color parameters of petals were measured; flavonoid composition and content as well as flavonoid biosynthetic gene expression were monitored. The results showed that daminozide treatment caused less red coloration in herbaceous Peony. The content of total anthocyanins was decreased by 23%, and the content of total anthoxanthins was increased by 8%; thus, it appeared that less red coloration in daminozide-treated flowers was associated with restricted anthocyanin accumulation. Furthermore, the gene expression patterns of flavonoid 3′-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase were lower after daminozide treatment, which could decrease anthocyanin accumulation and lead to less red coloration in flowers. These findings would improve our understanding of daminozide regulation of flower color in herbaceous Peony.
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an actin gene as the internal control for gene expression analysis in herbaceous Peony paeonia lactiflora pall
African Journal of Agricultural Research, 2012Co-Authors: Daqiu Zhao, Jintao GeAbstract:As an internal control gene, actin plays an important role in gene expression analysis. In this study, the full length cDNA and genomic DNA sequences of an actin gene were isolated from Chinese herbaceous Peony (Paeonia lactiflora Pall.) for the first time. Sequence analysis indicated that the full length of cDNA was 1657 bp, containing one open reading frame (ORF) of 1134 bp that encodes 377 amino acids, and the genomic DNA sequence was 1817 bp, containing three exons and two introns. They all had been submitted to GenBank and obtained the accession number JN105299 and FJ713744, respectively. Homology analysis showed that the cDNA and its deduced protein of P. lactiflora actin (Plactin) shared high similarities with those from other plants. Protein structure prediction showed that the deduced protein did not have signal peptide and transmembrane topological structure, and was a hydrophilic and stable protein. Temporal and spatial expression analysis of Plactin, which was performed by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR), displayed its stable expression which conformed to the regulation of using as an internal control gene. The results suggested that actin of herbaceous Peony could be used as an alternative internal control gene for the study of the targeted genes expression and regulation characteristics in herbaceous Peony. Key words: Paeonia lactiflora, actin, internal control gene, gene expression.
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herbaceous Peony paeonia lactiflora pall as an alternative source of oleanolic and ursolic acids
International Journal of Molecular Sciences, 2011Co-Authors: Chunhua Zhou, Daqiu Zhao, Ying Zhang, Yanle Sheng, Sansan Lv, Yue HuAbstract:Oleanolic acid (OA) and ursolic acid (UA) have been proven to possess many biological activities, and much attention is focused on the search for plants which are rich in OA and UA. In this report, the OA and UA accumulation characteristics were investigated in 47 cultivars of Chinese herbaceous Peony (Paeonia lactiflora Pall.) and were followed in three cultivars over different developmental stages as measured by high performance liquid chromatography (HPLC). OA and UA levels in leaves and stems demonstrated an overall upward trend from May 1 to September 15 except for UA in the leaves of “Hong Feng”. The maximum values of OA and UA in leaves of “Yangfei Chu Yu”, “Fen Zhu Pan” and “Hong Feng” were 852.98, 575.60, 290.48 μg/g FW and 924.94, 827.36, 432.67 μg/g FW, respectively. The maximum values of OA and UA in stems of “Yangfei Chu Yu”, “Fen Zhu Pan” and “Hong Feng” were 359.28, 90.49, 43.90 μg/g FW and 326.86, 82.25, 56.63 μg/g FW, respectively. OA and UA contents in leaves of 47 different herbaceous Peony cultivars ranged from 66.73–618.12 and 36.23–665.14 μg/g FW, respectively, with average values of 171.62 and 227.57 μg/g FW, respectively. The results suggested that the aboveground parts of herbaceous Peony may be used as an alternative source of OA and UA for medicinal purposes in addition to its ornamental purposes.
