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Songlin Li - One of the best experts on this subject based on the ideXlab platform.
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chemical markers for quality control of bran fried sulfur fumigated paeoniae radix alba
Journal of Pharmaceutical and Biomedical Analysis, 2018Co-Authors: Yayun Xu, Jindi Xu, Fang Long, Yeqing Zhang, Ming Kong, Songlin LiAbstract:Abstract In traditional Chinese medicine practice, crude herbs are often subjected to traditional processing (Paozhi in Chinese) for a special medicinal purpose. Bran-frying is one of processing methods for Paeoniae Radix Alba (PRA). Previous studies found that Paeoniflorin and Paeoniflorin sulfonate, a principle bioactive compound and its sulfur-fumigation induced characteristic sulfur-containing derivative, could be used together with sulfur dioxide residue as chemical markers for the quality control of sulfur-fumigated PRA crude material. In this paper, the feasibility of these three markers used for the quality control of bran-fried sulfur-fumigated PRA was further investigated. First, homemade samples of sulfur-fumigated PRA with different sulfur-fumigation duration (0.5–6 h) were bran-fried, and stored for 12 months. Second, the contents of sulfur dioxide residue, Paeoniflorin and Paeoniflorin sulfonate were dynamically quantified respectively. Third, the variation of the marker contents and their correlation during bran-frying and storage was evaluated. A validation was conducted using commercial bran-fried PRA samples. The results showed that bran-frying caused an averaged reduction of 20% in the content of sulfur dioxide residue, and during the first two months of storage the content of sulfur dioxide residue was decreased by up to 27%, then the content was tending towards stability for the subsequent ten months of storage (RSD = 3.92%). Meanwhile, Paeoniflorin and Paeoniflorin sulfonate were relatively stable, the contents of which were not affected by bran-frying processing and 12 months of storage. The correlations between the contents of sulfur dioxide residue and Paeoniflorin/Paeoniflorin sulfonate were obviously influenced by storage duration. Since sulfur dioxide residue is a safety marker, while Paeoniflorin and Paeoniflorin sulfonate can reflect the inner quality and the impact extent of sulfur-fumigation on the quality of bran-fried PRA respectively, these three chemicals might be used together as markers for the quality control, and consequently to ensure the safety and efficacy of bran-fried PRA.
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paeonifiorin sulfonate as a characteristic marker for specifically inspecting chinese patent medicine liu wei di huang wan contained sulfur fumigated moutan cortex
Journal of Pharmaceutical and Biomedical Analysis, 2017Co-Authors: Xiuyang Li, Jindi Xu, Fang Long, Yeqing Zhang, Ming Kong, Hong Shen, Songlin LiAbstract:Abstract Sulfur fumigation can induce chemical transformation of bioactive components, consequently the alteration of bioactivities or even toxicities of medicinal herbs. Inspecting Chinese patent medicines (CPM) contained sulfur-fumigated constituent herbs is crucial for ensuring the safety and efficacy of CPM. Paeonifiorin sulfonate is a sulfur-fumigation induced compound of Moutan Cortex (MC), one of the main constituent herbs of a commonly used CPM Liu-Wei-Di-Huang-Wan (LWDHW). Herein, we investigated the approach of paeonifiorin sulfonate as a characteristic marker for specifically inspecting LWDHW potentially contained sulfur-fumigated MC (SFMC). First, mimic LWDHW samples contained SFMC (SFMC-LWDHW) and non-fumigated MC (NFMC-LWDHW) were prepared respectively. Second, an LC–MS method was developed and validated to qualitatively and quantitatively determine paeonifiorin sulfonate in the mimic LWDHW samples. Third, the established method was applied to analyze the commercial LWDHW samples. The results showed that Paeoniflorin sulfonate could only be detectable in SFMC-LWDHW, but not in NFMC-LWDHW samples. The CPM matrix could enhance the response of Paeoniflorin sulfonate in mass spectrometry analysis. In addition, the LOQ, linearity, precision, accuracy and stability were also demonstrated to be acceptable for quantifying Paeoniflorin sulfonate in LWDHW. Commercial samples analysis indicated that Paeoniflorin sulfonate were detectable in 9 of 10 commercial LWDHW samples, with the content varied between 105.53 μg/g and 438.61 μg/g. All the results suggested that Paeoniflorin sulfonate could be used as a characteristic and reliable chemical marker for specifically inspecting commercial LWDHW contained SFMC. This study also provides a new strategy for the quality control of other CPMs potentially produced from sulfur-fumigated constituent herbs.
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detection of sulfur fumigated paeoniae alba radix in complex preparations by high performance liquid chromatography tandem mass spectrometry
Molecules, 2012Co-Authors: Jie Wu, Hong Shen, Jun Xu, Songlin LiAbstract:Detection of sulfur-fumigated Paeoniae Alba Radix (PAR) in different complex preparations is challenging due to the relatively lower content of PAR and interference from more complicated components in complex preparations with different multiple constituent herbs. In this study, a high performance liquid chromatography- triple-quadrupole tandem mass spectrometry method was developed for detecting sulfur-fumigated PAR in different complex preparations. Paeoniflorin, the major component of PAR, and Paeoniflorin sulfonate, the characteristic artifact transformed from Paeoniflorin during sulfur-fumigation of PAR, were used as chemical markers. Multiple reaction monitoring (MRM) scan was employed to maximize sensitivity and selectivity. Through optimizing full mass scan and daughter ion scan conditions, two mass transitions were selected and employed respectively for unequivocal identification of Paeoniflorin and Paeoniflorin sulfonate. The detection limits for Paeoniflorin and Paeoniflorin sulfonate using MRM were much lower than those detected with UV 270 nm. Paeoniflorin and Paeoniflorin sulfonate could be simultaneously detected in different commercial PAR-containing complex preparations without interference of other components using the established method, indicating that the newly established method was selective and sensitive enough for screening sulfur-fumigated PAR in commercial complex preparations.
Lingyi Kong - One of the best experts on this subject based on the ideXlab platform.
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Paeoniflorin attenuates amyloid beta peptide induced neurotoxicity by ameliorating oxidative stress and regulating the ngf mediated signaling in rats
Brain Research, 2013Co-Authors: Lvyi Chen, Qiang Fu, Weiwei Ji, Shuyuan Wang, Zhaohui Liang, Rong Qu, Lingyi KongAbstract:Abstract Paeoniflorin is a monoterpene glycoside isolated from the aqueous extract of the dry root of Paeonia. It has been identified to exhibit many pharmacological effects including enhancing the cognitive ability, producing anti-depressant-like effect and reducing the MTPT-induced toxicity. In our previous study, it has shown that Paeoniflorin improved the cognitive ability and attenuated the oxidative stress in the Aβ(1–42)-treated rats. In order to further elucidate the possible molecular mechanisms of Paeoniflorin on the cognitive ability, rats were injected with Aβ(1–42) (1 μg/μL) and later with Paeoniflorin (15 mg/kg and 30 mg/kg, i.p.) and donepezil hydrochloride (2 mg/kg, i.p.) daily for 20 days in this study. The results showed that the long-term treatment of Paeoniflorin or donepezil enhanced the cognitive performances in the Morris water maze test, restored the decreased activities of superoxide dismutase and catalase and the increased level of malondialdehyde, and reversed the alterations of matrix metallopeptidase-9 and tissue-inhibitor of metalloproteinase-1 in the hippocampus of Aβ(1–42)-treated rats. Paeoniflorin also up-regulated the activity of choline acetyltrasferase and the expression of tyrosine kinase A receptor, and down-regulated the activity of acetylcholine esterase in the hippocampus of Aβ(1–42)-treated rats. These results demonstrate that Paeoniflorin ameliorates the spatial learning and memory deficits by attenuating oxidative stress and regulating the nerve growth factor-mediated signaling to reinforce cholinergic functions in the hippocampus of the Aβ(1–42)-treated rats.
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comparative pharmacokinetics of Paeoniflorin in plasma of vascular dementia and normal rats orally administrated with danggui shaoyao san or pure Paeoniflorin
Fitoterapia, 2011Co-Authors: Junsong Wang, Lingyi KongAbstract:Danggui-Shaoyao-San (DSS), a traditional Chinese medicinal prescription, is clinically used for the treatment of vascular dementia (VD). This study compared the pharmacokinetics of Paeoniflorin, the main active ingredients in Paeonia lactiflora Pall, in VD and normal rats administrated with DSS or pure Paeoniflorin. The absorptions of Paeoniflorin in both VD and normal group of rats were better when orally administrated with DSS than that with pure Paeoniflorin, which justified the combined use as formula DSS. Moreover, a better absorption of Paeoniflorin in rats of the VD than in normal group was observed, which is helpful for the treatment of VD.
Yuefeng Li - One of the best experts on this subject based on the ideXlab platform.
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Study on the effects of Paeoniflorin in Sini San on sleep time
2011 International Conference on Remote Sensing Environment and Transportation Engineering, 2011Co-Authors: Yuefeng LiAbstract:To study on the effects of Paeoniflorin in Sini San on sleep time Method: Paeoniflorin in Sini San is studied by the method of prolonging sleep time in mice made by sodium pentobarbital. Results: Paeoniflorin in Sini San can increase duration of mice sleep time evidently. Conclusion: Study on the effects of Paeoniflorin in Sini San on sleep time provided a basic data for the further studies on effective constituents and the effecting mechanism of Sini San.
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Study on the effects of Paeoniflorin in Sini San on sleep time
2011 International Conference on Remote Sensing Environment and Transportation Engineering, 2011Co-Authors: Yuefeng LiAbstract:0bjective: To study on the effects of Paeoniflorin in Sini San on sleep time o Method: Paeoniflorin in Sini San is studied by the method of prolonging sleep time in mice made by sodium pentobarbital. Results: Paeoniflorin in Sini San can increase duration of mice sleep time evidently. Conclusion: Study on the effects of Paeoniflorin in Sini San on sleep time provided a basic data for the further studies on effective constituents and the effecting mechanism of Sini San.
Hong Shen - One of the best experts on this subject based on the ideXlab platform.
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protective effects of Paeoniflorin on tnbs induced ulcerative colitis through inhibiting nf kappab pathway and apoptosis in mice
International Immunopharmacology, 2017Co-Authors: Peiqing Gu, Lu Zhang, Hong ShenAbstract:Abstract Paeoniflorin is traditionally used to treat inflammatory disorders. In our laboratory, we have scientifically validated the anti-inflammatory effect of Paeoniflorin. In this study, it has been aimed to evaluate in vivo anti-inflammatory effect of Paeoniflorin isolated from the dried peeled root of Paeonia lactiflora Pall. It was further intended to find out the probable mechanism of anti-inflammatory effect of Paeoniflorin. The anti-inflammatory effect of Paeoniflorin (15, 30 and 45 mg/kg) was measured employing TNBS-induced ulcerative colitis model of acute inflammation. The TNBS injection resulted significant colitis formation when compared with un-injected mice. The anti-inflammatory effects of Paeoniflorin for ulcerative colitis were assessed by body weight, colonic weight and length, macroscopic scores, and histopathological examinations. In addition, the colonic tissue levels of inflammation markers, including myeloperoxidase (MPO), IL-2, IL-6, IL-10, IL-12, IL-1β, TNF-α and IFN-γ were also determined to assess the effect of Paeoniflorin. In addition, western blot demonstrated that Paeoniflorin inhibited NF-kappaB signaling pathway and apoptosis in TNBS-induced ulcerative colitis tissues. In conclusion, all the findings of this study suggested that Paeoniflorin has the anti-inflammatory effect in ulcerative colitis via inhibiting MAPK/NF-kappaB pathway and apoptosis in mice.
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paeonifiorin sulfonate as a characteristic marker for specifically inspecting chinese patent medicine liu wei di huang wan contained sulfur fumigated moutan cortex
Journal of Pharmaceutical and Biomedical Analysis, 2017Co-Authors: Xiuyang Li, Jindi Xu, Fang Long, Yeqing Zhang, Ming Kong, Hong Shen, Songlin LiAbstract:Abstract Sulfur fumigation can induce chemical transformation of bioactive components, consequently the alteration of bioactivities or even toxicities of medicinal herbs. Inspecting Chinese patent medicines (CPM) contained sulfur-fumigated constituent herbs is crucial for ensuring the safety and efficacy of CPM. Paeonifiorin sulfonate is a sulfur-fumigation induced compound of Moutan Cortex (MC), one of the main constituent herbs of a commonly used CPM Liu-Wei-Di-Huang-Wan (LWDHW). Herein, we investigated the approach of paeonifiorin sulfonate as a characteristic marker for specifically inspecting LWDHW potentially contained sulfur-fumigated MC (SFMC). First, mimic LWDHW samples contained SFMC (SFMC-LWDHW) and non-fumigated MC (NFMC-LWDHW) were prepared respectively. Second, an LC–MS method was developed and validated to qualitatively and quantitatively determine paeonifiorin sulfonate in the mimic LWDHW samples. Third, the established method was applied to analyze the commercial LWDHW samples. The results showed that Paeoniflorin sulfonate could only be detectable in SFMC-LWDHW, but not in NFMC-LWDHW samples. The CPM matrix could enhance the response of Paeoniflorin sulfonate in mass spectrometry analysis. In addition, the LOQ, linearity, precision, accuracy and stability were also demonstrated to be acceptable for quantifying Paeoniflorin sulfonate in LWDHW. Commercial samples analysis indicated that Paeoniflorin sulfonate were detectable in 9 of 10 commercial LWDHW samples, with the content varied between 105.53 μg/g and 438.61 μg/g. All the results suggested that Paeoniflorin sulfonate could be used as a characteristic and reliable chemical marker for specifically inspecting commercial LWDHW contained SFMC. This study also provides a new strategy for the quality control of other CPMs potentially produced from sulfur-fumigated constituent herbs.
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detection of sulfur fumigated paeoniae alba radix in complex preparations by high performance liquid chromatography tandem mass spectrometry
Molecules, 2012Co-Authors: Jie Wu, Hong Shen, Jun Xu, Songlin LiAbstract:Detection of sulfur-fumigated Paeoniae Alba Radix (PAR) in different complex preparations is challenging due to the relatively lower content of PAR and interference from more complicated components in complex preparations with different multiple constituent herbs. In this study, a high performance liquid chromatography- triple-quadrupole tandem mass spectrometry method was developed for detecting sulfur-fumigated PAR in different complex preparations. Paeoniflorin, the major component of PAR, and Paeoniflorin sulfonate, the characteristic artifact transformed from Paeoniflorin during sulfur-fumigation of PAR, were used as chemical markers. Multiple reaction monitoring (MRM) scan was employed to maximize sensitivity and selectivity. Through optimizing full mass scan and daughter ion scan conditions, two mass transitions were selected and employed respectively for unequivocal identification of Paeoniflorin and Paeoniflorin sulfonate. The detection limits for Paeoniflorin and Paeoniflorin sulfonate using MRM were much lower than those detected with UV 270 nm. Paeoniflorin and Paeoniflorin sulfonate could be simultaneously detected in different commercial PAR-containing complex preparations without interference of other components using the established method, indicating that the newly established method was selective and sensitive enough for screening sulfur-fumigated PAR in commercial complex preparations.
Hongbo Wang - One of the best experts on this subject based on the ideXlab platform.
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sensitive analysis and pharmacokinetic study of the isomers Paeoniflorin and albiflorin after oral administration of total glucosides of white paeony capsule in rats
Journal of Chromatography B, 2016Co-Authors: Hailing Yang, Ying Peng, Pei Wang, Shuyao Wang, Yuqing Zhao, Jingqiu Huang, Xiaoyi Yu, Siqi Feng, Na Yang, Hongbo WangAbstract:Abstract A sensitive and reliable method using liquid chromatography tandem mass spectrometry (LC–MS/MS) was established for the simultaneous assay of Paeoniflorin and albiflorin in bio-samples of rats after liquid–liquid extraction with ethylacetate. For the first time, the developed method was validated and successfully applied to the pharmacokinetics study of Paeoniflorin and albiflorin after oral administration of Total Glucosides Of White Paeony Capsule (TGP). Relative to the intravenous injection, the absolute bio-availabilities of Paeoniflorin and albiflorin were 2.8 and 1.7%, while their excretion in feces was 43.06 and 40.87%, respectively. Both Paeoniflorin and albiflorin showed dose-dependent exposure in plasma, with a half-life of approximately 1.8 h. No significant differences were observed between a single equal dose of Paeoniflorin or albiflorin and that of TGP for the pharmacokinetic parameters, including AUC, T1/2 and Cmax. Paeoniflorin and albiflorin were exposed at high levels in immune relevant organ/tissues, such as the spleen, thymus and bone, which could facilitate immuno-regulatory activities.
