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Donald C. Behringer - One of the best experts on this subject based on the ideXlab platform.
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white spot syndrome virus and the caribbean spiny lobster Panulirus Argus susceptibility and behavioral immunity
Journal of Invertebrate Pathology, 2019Co-Authors: Erica P Ross, Donald C. Behringer, Jamie BojkoAbstract:The Caribbean spiny lobster Panulirus Argus is susceptible to infection by Panulirus Argus Virus 1 (PaV1), the only virus known to naturally infect any lobster species. However, P. Argus is able to mitigate PaV1 transmission risk by avoiding infected individuals. P. Argus may also be susceptible to another lethal virus, White Spot Syndrome Virus (WSSV). WSSV has not been documented in wild populations of spiny lobsters, but has been experimentally transmitted to six other lobster species from the genus Panulirus. Although WSSV has been detected intermittently in wild populations of shrimp in the Caribbean region, the risk to P. Argus has not been evaluated. Potential emergence of the disease could result in fisheries losses and ecological disruption. To assess the risk to P. Argus, we tested its susceptibility to WSSV via injection and waterborne transmission. We also tested whether healthy lobsters can detect and avoid conspecifics with qPCR-quantifiable WSSV infections. We found P. Argus to be highly susceptible to WSSV via intramuscular injection, with mortality reaching 88% four weeks post inoculation. Panulirus Argus was also susceptible to WSSV via waterborne transmission, but WSSV burden was low after four weeks via qPCR. Behavioral assays indicated that P. Argus can detect and avoid conspecifics infected with WSSV and the avoidance response was strongest for the most heavily infected individuals - a response comparable to PaV1-infected conspecifics. Panulirus Argus is the first spiny lobster found to be susceptible to WSSV in the Americas, but it is possible that a generalized avoidance response by healthy lobsters against infected conspecifics provides a behavioral defense and may reduce WSSV infection potential and prevalence. Preliminary evidence suggests that the infiltration of hemolymph constituents into the urine may be the source of the avoidance behavior and could therefore extend to other directly transmitted pathogens in spiny lobster populations preventing them from becoming common in their population.
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white spot syndrome virus and the caribbean spiny lobster Panulirus Argus susceptibility and behavioral immunity
bioRxiv, 2018Co-Authors: Erica P Ross, Donald C. Behringer, Jamie BojkoAbstract:Abstract The Caribbean spiny lobster Panulirus Argus is susceptible to infection by Panulirus Argus Virus 1 (PaV1), the only virus known to naturally infect any lobster species. However, P. Argus is able to mitigate PaV1 transmission risk by avoiding infected individuals. White Spot Syndrome Virus (WSSV) has a particularly wide host range. WSSV has not been documented in wild populations of spiny lobsters, but has been experimentally transmitted to six other lobster species from the genus Panulirus spp. While WSSV has been detected intermittently in wild populations of shrimp in the Caribbean region, the risk to P. Argus has not been evaluated. Potential emergence of the disease could result in fisheries losses and ecological disruption. To assess the risk to P. Argus, we tested its susceptibility to WSSV via injection and waterborne transmission. We also tested whether healthy lobsters can detect and avoid conspecifics with qPCR-quantifiable WSSV infections. We found P. Argus to be highly susceptible to WSSV via intramuscular injection, with mortality reaching 88% four weeks post inoculation. Panulirus Argus was also susceptible to WSSV via waterborne transmission, but WSSV burden was low after four weeks via qPCR. Behavioral assays indicated that P. Argus can detect and avoid conspecifics infected with WSSV and the avoidance response was strongest for the most heavily infected individuals – a response comparable to PaV1-infected conspecifics. Panulirus Argus is the first spiny lobster found to be susceptible to WSSV in the Americas, but it is possible that a generalized avoidance response by healthy lobsters against infected conspecifics provides a behavioral defense and may reduce WSSV infection potential and prevalence. Such avoidance may extend to other directly transmitted pathogens in spiny lobster populations preventing them from becoming common in their population. Author Summary Erica P. Ross is a PhD candidate at the University of Florida, studying the disease ecology of the Caribbean spiny lobster, with a focus on chemosensory ecology. Donald C. Behringer is an associate professor at the University of Florida and his research focuses on disease ecology, epidemiology, and fishery ecology, with a focus on crustaceans and other marine invertebrates. Jamie Bojko received his PhD from the University of Leeds and is currently a post-doctorate associate at the University of Florida studying experimental and systemic crustacean pathology.
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partial validation of a taqman real time quantitative pcr assay for the detection of Panulirus Argus virus 1
Diseases of Aquatic Organisms, 2018Co-Authors: Abigail S Clark, Donald C. Behringer, Jessica Moss Small, Thomas B WaltzekAbstract:The Caribbean spiny lobster Panulirus Argus supports important fisheries throughout the greater Caribbean and is also the only known host for the pathogenic virus Panulirus Argus virus 1 (PaV1). While discovered nearly 2 decades ago, gaps still exist in our knowledge of PaV1, such as the dose required to establish infection and its viability outside of the host. To help answer such questions and to enhance diagnostic capabilities, we developed a TaqMan real-time quantitative polymerase chain reaction (qPCR) assay for PaV1. Of the advantages offered by qPCR, one of the most important benefits is its ability to accurately quantify viral DNA copies in a clinical sample. The qPCR assay was found to be efficient (mean ± SD: 99.19 ± 4.67%) and sensitive, detecting as few as 10 copies of PaV1 plasmid DNA. Its diagnostic sensitivity and specificity determined using a set of 165 lobster samples (138 from Florida, USA, and 27 from across the Caribbean) were 100 and 84%, respectively. The qPCR assay should thus prove useful as a research tool and for detecting and quantifying PaV1 infection severity in Caribbean spiny lobsters.
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distribution prevalence and genetic analysis of Panulirus Argus virus 1 pav1 from the caribbean sea
Diseases of Aquatic Organisms, 2013Co-Authors: Jessica Moss, Donald C. Behringer, Jeffrey D. Shields, Antonio Baeza, Alfonso Aguilarperera, Phillippe G Bush, Clement Dromer, Alejandro Herreramoreno, Lester Gittens, Thomas R MatthewsAbstract:The pathogenic virus Panulirus Argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus Argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We col- lected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence was lowest in the south- western Caribbean. DNA sequence analysis was performed on a fragment of the viral DNA to examine the genetic diversity of PaV1 on a Caribbean-wide scale. Sequence variation in the viral DNA fragment was high, with 61 unique alleles identified from 9 areas. The sharing of viral alleles in lobsters from distant locations supports the hypothesis of a strong genetic connectivity among lobsters within the Caribbean, and further supports the hypothesis that postlarvae infected with PaV1 may serve to disperse the virus over long distances.
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spatial dynamics in the social lobster Panulirus Argus in response to diseased conspecifics
Marine Ecology Progress Series, 2013Co-Authors: Joshua R Anderson, Donald C. BehringerAbstract:Pathogens can influence the behaviors of their hosts, including avoidance by healthy individuals. However, the spatial structuring consequences of these behaviors are often unknown, especially in the marine environment. The Caribbean spiny lobster Panulirus Argus (Latreille, 1804) avoids sheltering with conspecifics infected with P. Argus virus 1 (PaV1). We sought to deter- mine the behavioral cue and if this behavior alters population spatial structure in the wild. We used a series of laboratory Y-maze experiments to determine the mechanism of detection and also employed artificial shelter arrays placed in different flow conditions in the field to determine the effect of diseased lobster aversion on healthy lobster spatial dynamics. Results showed that avoid- ance is driven by the chemoreception of cues based in the urine. The chemical cue alone was as effective as having a diseased lobster present and visible. The presence of single PaV1-infected lobsters altered the small-scale spatial structure of wild populations, especially under low flow regimes, via redistribution of neighboring individuals. In shelter-limited environments, disease avoidance has the potential to increase juvenile mortality, as nearby conspecifics are displaced from dens and exposed to a higher risk of predation.
Jeffrey D. Shields - One of the best experts on this subject based on the ideXlab platform.
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Appendix A. Supplemental testing, sensitivity, and results for the Panulirus Argus-PaV1 model.
2016Co-Authors: Thomas W. Dolan, Mark J Butler, Jeffrey D. ShieldsAbstract:Supplemental testing, sensitivity, and results for the Panulirus Argus-PaV1 model
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Supplement 1. C++ code for the Panulirus Argus-PaV1 spatially explicit, individual-based model.
2016Co-Authors: Thomas W. Dolan, Mark J Butler, Jeffrey D. ShieldsAbstract:File List Supplement_Model_Source_Code.txt (MD5: 4ee8fd3f75fef441a16d2db546f1c767) Description The file, Supplement_Model_Source_Code.txt, contains a complete source listing of C++ data and control structures for the Panulirus Argus-PaV1 model. The model was programmed in Microsoft Visual Studio 2008. The various modules in the source listing may be copied into any development environment to be compiled, but GUI elements could not be included, and no effort has been made to ensure portability.
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distribution prevalence and genetic analysis of Panulirus Argus virus 1 pav1 from the caribbean sea
Diseases of Aquatic Organisms, 2013Co-Authors: Jessica Moss, Donald C. Behringer, Jeffrey D. Shields, Antonio Baeza, Alfonso Aguilarperera, Phillippe G Bush, Clement Dromer, Alejandro Herreramoreno, Lester Gittens, Thomas R MatthewsAbstract:The pathogenic virus Panulirus Argus virus 1 (PaV1) was first discovered in Caribbean spiny lobsters Panulirus Argus from the Florida Keys (USA) in 1999 and has since been reported in Belize, Mexico, and Cuba; its distribution in the wider Caribbean is unknown. We col- lected tissue samples from adult spiny lobsters from 30 locations in 14 countries bordering the Caribbean Sea and used molecular diagnostics to assay for the presence of PaV1. PaV1 occurred primarily in the northern areas of the Caribbean, where its prevalence was highest. The virus was not found in lobsters from the southeastern Caribbean, and its prevalence was lowest in the south- western Caribbean. DNA sequence analysis was performed on a fragment of the viral DNA to examine the genetic diversity of PaV1 on a Caribbean-wide scale. Sequence variation in the viral DNA fragment was high, with 61 unique alleles identified from 9 areas. The sharing of viral alleles in lobsters from distant locations supports the hypothesis of a strong genetic connectivity among lobsters within the Caribbean, and further supports the hypothesis that postlarvae infected with PaV1 may serve to disperse the virus over long distances.
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pav1 infection in the florida spiny lobster Panulirus Argus fishery and its effects on trap function and disease transmission
Canadian Journal of Fisheries and Aquatic Sciences, 2012Co-Authors: Donald C. Behringer, Mark J Butler, Jessica Moss, Jeffrey D. ShieldsAbstract:The Caribbean spiny lobster (Panulirus Argus) supports the most economically valuable fishery in the Caribbean. In Florida, USA, the majority of the catch is landed in traps "baited" with live, sublegal-sized lobsters that attract other lob- sters due to their social nature. This species is also commonly infected by the pathogenic virus Panulirus Argus Virus 1 (PaV1). Here we describe a polymerase chain reaction (PCR) based assessment of the prevalence of PaV1 in the lobster fishery from the Florida Keys. We tested the effect of PaV1-infected lobsters in traps on catch and on transmission to other trapped, uninfected lobsters. We found that 11% of the lobsters caught in commercial traps were positive for the virus by PCR, but none of these animals showed visible signs of disease. We also tested whether healthy lobsters avoid diseased lob- sters in traps. Traps into which we introduced an infected lobster caught significantly fewer lobsters than traps containing an uninfected lobster. Moreover, uninfected lobsters confined in traps with infected lobsters acquired significantly more PaV1 infections than those confined with uninfected lobsters. This study demonstrates the indirect effects that pathogens can have on fisheries and the unintended consequences of certain fishery practices on the epidemiology of a marine pathogen.
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review of Panulirus Argus virus 1 a decade after its discovery
Diseases of Aquatic Organisms, 2011Co-Authors: Donald C. Behringer, Mark J Butler, Jeffrey D. Shields, Jessica MossAbstract:In 2000, a pathogenic virus was discovered in juvenile Caribbean spiny lobsters Pan- ulirus Argus from the Florida Keys, USA. Panulirus Argus virus 1 (PaV1) is the first naturally occurring pathogenic virus reported from lobsters, and it profoundly affects their ecology and physiology. PaV1 is widespread in the Caribbean with infections reported in Florida (USA), St. Croix, St. Kitts, Yucatan (Mexico), Belize, and Cuba. It is most prevalent and nearly always lethal in the smallest juvenile lob- sters, but this declines with increasing lobster size; adults harbor the virus, but do not present the characteristic signs of the disease. No other PaV1 hosts are known. The prevalence of PaV1 in juve- nile lobsters from the Florida Keys has been stable since 1999, but has risen to nearly 11% in the east- ern Yucatan since 2001. Heavily infected lobsters become sedentary, cease feeding, and die of meta- bolic exhaustion. Experimental routes of viral transmission include ingestion, contact, and for newly settled juveniles, free virus particles in seawater. Prior to infectiousness, healthy lobsters tend to avoid diseased lobsters and so infected juvenile lobsters mostly dwell alone, which appears to reduce disease transmission. However, avoidance of diseased individuals may result in increased shelter competition between healthy and diseased lobsters, and greater predation on infected lobsters. Little is known about PaV1 outside of Mexico and the USA, but it poses a potential threat to P. Argus fish- eries throughout the Caribbean.
Enrique Lozanoalvarez - One of the best experts on this subject based on the ideXlab platform.
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energy cost of the onshore transport of postlarvae of the caribbean spiny lobster Panulirus Argus
Bulletin of Marine Science, 2018Co-Authors: Ali F Espinosamagana, Patricia Brionesfourzan, Andrew Jeffs, Enrique LozanoalvarezAbstract:Recent studies suggest that energy reserves play a crucial role in the recruitment of postlarval spiny lobsters. After a protracted pelagic larval phase that develops in oceanic waters, the final stage phyllosoma larva metamorphoses into a non-feeding postlarva (puerulus) that actively swims shoreward to settle in shallow coastal habitats and does not resume feeding until after the molt into juvenile. We examined the content of total protein (TP), total lipid (TL), and lipid classes in five developmental stages of the spiny lobster, Panulirus Argus (Latreille, 1804), involved in the transition from pelagic to benthic life: final stage phyllosomata, nektonic pueruli, transparent benthic pueruli (newly settled), pigmented pueruli, and first-instar juveniles; collected in autumn 2012 and spring 2013. TL decreased progressively with development, from 26% of dry weight in phyllosomata to 7% in juveniles in autumn (a 73% decrease), and from 25% to 6% in spring (a 76% decrease). In all stages, phospholipid accounted for approximately 80%–87% of TL. TP was higher in all three pueruli substages than in phyllosomata and juveniles. Although season did not significantly affect either TL or TP content, the decline in TL between final phyllosomata and nektonic pueruli was greater in autumn, suggesting a greater energy cost of metamorphosis at warmer temperatures, whereas the decline in TL between nektonic and transparent benthic pueruli was more marked in spring, when the Yucatan Current is stronger, potentially increasing the energy cost of shoreward swimming. These findings may partially explain the interannual seasonal settlement patterns observed in this species.
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variability in prevalence of cymatocarpus solearis trematoda brachycoeliidae in caribbean spiny lobsters Panulirus Argus decapoda palinuridae from bahia de la ascension mexico
Journal of Invertebrate Pathology, 2016Co-Authors: Patricia Brionesfourzan, Ruben Munoz De Cotehernandez, Enrique LozanoalvarezAbstract:Abstract Cymatocarpus solearis, a brachycoeliid trematode that parasitizes sea turtles, uses spiny lobsters Panulirus Argus as second intermediate hosts. We examined variability in infection by C. solearis in Bahia de la Ascension, Mexico, a tropical bay with a highly productive fishery for P. Argus that is based on the extensive use of casitas (large artificial shelters), which can harbor multiple juveniles and adults of this gregarious lobster species. We sampled 3956 lobsters from 466 casitas distributed over three bay zones within two closed seasons and one fishing season. In these lobsters (9.5–115.2 mm carapace length, CL), the average infection prevalence was 11.7% but the probability of infection increased significantly with size. Prevalence varied with season but was consistently higher in one zone than in the other two zones. Infection with C. solearis was negatively related with clinical infection with Panulirus Argus Virus 1 (PaV1). We also sampled 405 lobsters from the commercial catch obtained throughout the bay at the onset of the fishing season. In these relatively larger lobsters (70.3–168.0 mm CL), average prevalence of C. solearis was higher (23.5%) but was not affected by lobster size or sex. Encysted metacercariae occurred in both abdominal and cephalothoracic muscles. The effects of C. solearis on phenotypic traits of P. Argus that may potentially impact the host population dynamics and fisheries remain to be investigated.
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genetic analysis reveals temporal population structure in caribbean spiny lobster Panulirus Argus within marine protected areas in mexico
Fisheries Research, 2015Co-Authors: Patricia Brionesfourzan, Enrique Lozanoalvarez, Nathan K Truelove, Kim Leycooper, Iris Seguragarcia, Bruce F Phillips, Stephen J Box, Richard F PreziosiAbstract:Abstract Management efforts for improving the sustainability of the Caribbean spiny lobster (Panulirus Argus) fishery require knowledge of population connectivity. The aim of this study is to investigate population connectivity of P. Argus at two levels: (1) spatially between two marine protected areas (MPAs) in the Caribbean coast of Mexico, and (2) temporally within MPAs; by genotyping discrete size classes lobsters using microsatellite markers. No evidence of population differentiation between lobster populations from Banco Chinchorro and Sian Ka’an MPAs was found (P = 0.139). In contrast significant levels of population differentiation among discrete size classes of lobsters was found (FST = 0.0054; P = 0.0052). Temporal variation among the genotypes of new larval recruits may explain these results. Future research will be required to directly test the genotypes of new larval recruits in Banco Chinchorro and Sian Ka’an MPAs to confirm this hypothesis.
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Panulirus Argus virus 1 pav1 infection prevalence and risk factors in a mexican lobster fishery employing casitas
Diseases of Aquatic Organisms, 2013Co-Authors: Juan Pablo Huchinmian, Patricia Brionesfourzan, Rossanna Rodriguezcanul, Enrique LozanoalvarezAbstract:In Bahia de la Ascension in Mexico, the Caribbean spiny lobster Panulirus Argus fish- ery is based on extensive use of artificial shelters (casitas) that can harbor both juveniles and adults of this highly gregarious species. There is concern that the use of casitas might increase contact transmission of Panulirus Argus virus 1 (PaV1). However, a previous study found no evi- dence for lobster crowding within casitas influencing the prevalence of clinical PaV1 disease, although differences in clinical prevalence were noted between different bay environments. To investigate this more closely, 683 lobsters were sampled from casitas in 2 zones in this bay (Vigia Chico, a shallow low-vegetation zone, and Punta Allen, a deeper dense-vegetation zone) previ- ously found to have the lowest and highest prevalence, respectively, of observed clinical signs. When hemolymph collected from these lobsters was tested by PCR, the prevalence of PaV1 infec- tion was found to be significantly lower in Vigia Chico relative to Punta Allen irrespective of sea- son or the size, sex, or presence of shell injuries on lobsters. Among 714 large commercial-catch lobsters collected throughout the bay, the prevalence of infection was low irrespective of year or sex. For all lobsters tested, the sensitivity (0.510) at which PaV1 infection was detected by observed clinical signs was about half that determined by PCR, but the specificity of clinical signs was absolute (1), indicating that a simple 2× correction factor can be used to accurately estimate PaV1 infection prevalence based on more easily conducted visual assessments of lobsters.
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estimates of exploitation rates of the spiny lobster fishery for Panulirus Argus from tagging within the bahia espiritu santa sian ka an biosphere reserve mexican caribbean
Marine Biology Research, 2013Co-Authors: K Leycooper, S De Lestang, Bruce Phillips, Enrique LozanoalvarezAbstract:Abstract The Caribbean spiny lobster (Panulirus Argus) fishery is currently being assessed for a certification process. It is the main economic activity within the Biosphere Reserve of Sian Ka'an-Mexico (SK), which is a marine-protected area where restricted access fishing is allowed. In this study, commercial catch rates were examined, and lobsters over a wide size range were tagged throughout the 2010/2011 fishing season, to assess fishing mortality rates and movement patterns in Bahia Espiritu Santo-SK. Lobster tag recovery data were aggregated into 2-week periods and analysed using a modified Brownie model that was parameterized to account for lobster tag-reporting, and the lobster tag-retention rates. This allowed the estimation of instantaneous rates of natural and fishing mortality, considering catchability and fishing effort. Independent aquaria trials were conducted to better estimate lobster tag retention, tagging-induced mortality and interviews with fishermen were conducted to better estimate ...
Patricia Brionesfourzan - One of the best experts on this subject based on the ideXlab platform.
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energy cost of the onshore transport of postlarvae of the caribbean spiny lobster Panulirus Argus
Bulletin of Marine Science, 2018Co-Authors: Ali F Espinosamagana, Patricia Brionesfourzan, Andrew Jeffs, Enrique LozanoalvarezAbstract:Recent studies suggest that energy reserves play a crucial role in the recruitment of postlarval spiny lobsters. After a protracted pelagic larval phase that develops in oceanic waters, the final stage phyllosoma larva metamorphoses into a non-feeding postlarva (puerulus) that actively swims shoreward to settle in shallow coastal habitats and does not resume feeding until after the molt into juvenile. We examined the content of total protein (TP), total lipid (TL), and lipid classes in five developmental stages of the spiny lobster, Panulirus Argus (Latreille, 1804), involved in the transition from pelagic to benthic life: final stage phyllosomata, nektonic pueruli, transparent benthic pueruli (newly settled), pigmented pueruli, and first-instar juveniles; collected in autumn 2012 and spring 2013. TL decreased progressively with development, from 26% of dry weight in phyllosomata to 7% in juveniles in autumn (a 73% decrease), and from 25% to 6% in spring (a 76% decrease). In all stages, phospholipid accounted for approximately 80%–87% of TL. TP was higher in all three pueruli substages than in phyllosomata and juveniles. Although season did not significantly affect either TL or TP content, the decline in TL between final phyllosomata and nektonic pueruli was greater in autumn, suggesting a greater energy cost of metamorphosis at warmer temperatures, whereas the decline in TL between nektonic and transparent benthic pueruli was more marked in spring, when the Yucatan Current is stronger, potentially increasing the energy cost of shoreward swimming. These findings may partially explain the interannual seasonal settlement patterns observed in this species.
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variability in prevalence of cymatocarpus solearis trematoda brachycoeliidae in caribbean spiny lobsters Panulirus Argus decapoda palinuridae from bahia de la ascension mexico
Journal of Invertebrate Pathology, 2016Co-Authors: Patricia Brionesfourzan, Ruben Munoz De Cotehernandez, Enrique LozanoalvarezAbstract:Abstract Cymatocarpus solearis, a brachycoeliid trematode that parasitizes sea turtles, uses spiny lobsters Panulirus Argus as second intermediate hosts. We examined variability in infection by C. solearis in Bahia de la Ascension, Mexico, a tropical bay with a highly productive fishery for P. Argus that is based on the extensive use of casitas (large artificial shelters), which can harbor multiple juveniles and adults of this gregarious lobster species. We sampled 3956 lobsters from 466 casitas distributed over three bay zones within two closed seasons and one fishing season. In these lobsters (9.5–115.2 mm carapace length, CL), the average infection prevalence was 11.7% but the probability of infection increased significantly with size. Prevalence varied with season but was consistently higher in one zone than in the other two zones. Infection with C. solearis was negatively related with clinical infection with Panulirus Argus Virus 1 (PaV1). We also sampled 405 lobsters from the commercial catch obtained throughout the bay at the onset of the fishing season. In these relatively larger lobsters (70.3–168.0 mm CL), average prevalence of C. solearis was higher (23.5%) but was not affected by lobster size or sex. Encysted metacercariae occurred in both abdominal and cephalothoracic muscles. The effects of C. solearis on phenotypic traits of P. Argus that may potentially impact the host population dynamics and fisheries remain to be investigated.
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genetic analysis reveals temporal population structure in caribbean spiny lobster Panulirus Argus within marine protected areas in mexico
Fisheries Research, 2015Co-Authors: Patricia Brionesfourzan, Enrique Lozanoalvarez, Nathan K Truelove, Kim Leycooper, Iris Seguragarcia, Bruce F Phillips, Stephen J Box, Richard F PreziosiAbstract:Abstract Management efforts for improving the sustainability of the Caribbean spiny lobster (Panulirus Argus) fishery require knowledge of population connectivity. The aim of this study is to investigate population connectivity of P. Argus at two levels: (1) spatially between two marine protected areas (MPAs) in the Caribbean coast of Mexico, and (2) temporally within MPAs; by genotyping discrete size classes lobsters using microsatellite markers. No evidence of population differentiation between lobster populations from Banco Chinchorro and Sian Ka’an MPAs was found (P = 0.139). In contrast significant levels of population differentiation among discrete size classes of lobsters was found (FST = 0.0054; P = 0.0052). Temporal variation among the genotypes of new larval recruits may explain these results. Future research will be required to directly test the genotypes of new larval recruits in Banco Chinchorro and Sian Ka’an MPAs to confirm this hypothesis.
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Panulirus Argus virus 1 pav1 infection prevalence and risk factors in a mexican lobster fishery employing casitas
Diseases of Aquatic Organisms, 2013Co-Authors: Juan Pablo Huchinmian, Patricia Brionesfourzan, Rossanna Rodriguezcanul, Enrique LozanoalvarezAbstract:In Bahia de la Ascension in Mexico, the Caribbean spiny lobster Panulirus Argus fish- ery is based on extensive use of artificial shelters (casitas) that can harbor both juveniles and adults of this highly gregarious species. There is concern that the use of casitas might increase contact transmission of Panulirus Argus virus 1 (PaV1). However, a previous study found no evi- dence for lobster crowding within casitas influencing the prevalence of clinical PaV1 disease, although differences in clinical prevalence were noted between different bay environments. To investigate this more closely, 683 lobsters were sampled from casitas in 2 zones in this bay (Vigia Chico, a shallow low-vegetation zone, and Punta Allen, a deeper dense-vegetation zone) previ- ously found to have the lowest and highest prevalence, respectively, of observed clinical signs. When hemolymph collected from these lobsters was tested by PCR, the prevalence of PaV1 infec- tion was found to be significantly lower in Vigia Chico relative to Punta Allen irrespective of sea- son or the size, sex, or presence of shell injuries on lobsters. Among 714 large commercial-catch lobsters collected throughout the bay, the prevalence of infection was low irrespective of year or sex. For all lobsters tested, the sensitivity (0.510) at which PaV1 infection was detected by observed clinical signs was about half that determined by PCR, but the specificity of clinical signs was absolute (1), indicating that a simple 2× correction factor can be used to accurately estimate PaV1 infection prevalence based on more easily conducted visual assessments of lobsters.
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detection of Panulirus Argus virus 1 pav1 in exported frozen tails of subadult adult caribbean spiny lobsters Panulirus Argus
Diseases of Aquatic Organisms, 2009Co-Authors: Juan Pablo Huchinmian, Patricia Brionesfourzan, Enrique Lozanoalvarez, Raul Simaalvarez, Yanis Cruzquintana, Juan Antonio Perezvega, Cristina Pascualjimenez, Rossanna RodriguezcanulAbstract:The Caribbean spiny lobster Panulirus Argus is a valuable fishing resource and the trade in frozen lobster tails is an important industry. However, the presence of the pathogenic virus Panulirus Argus Virus 1 (PaV1), which causes systemic infection in P. Argus and is particularly lethal to juvenile individuals, has not been previously examined in imported/exported lobster products. We used PCR assays to determine the presence of PaV1 in abdominal muscle tissue of 22 frozen P. Argus tails exported from Belize to Mexico. Based on their size, the tails belonged to subadult-adult lob- sters. Using specific primers targeted for PaV1 resulted in 11 tails showing a specific 499 bp band. The sequence of positive amplified fragments showed a high similarity to PaV1 (95% identity with GenBank accession no. EF206313.1). Although the pathogenicity of PaV1 was not evaluated in the present study, our results provide the first evidence of PaV1 in frozen lobster tails exported in the seafood industry as well as the first molecular evidence of PaV1 in adult lobsters.
Norberto Capetillo - One of the best experts on this subject based on the ideXlab platform.
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Influencia del ciclo lunar en la captura de langosta espinosa Panulirus Argus (Decapoda: Palinuridae) en el Golfo de Batabanó, Cuba
Vicerractoría Investigación, 2011Co-Authors: Alexander Lopeztegui, Julio A Baisre, Norberto CapetilloAbstract:Muchos procesos biológicos tales como los comportamientos reproductivos y migratorios se han asociado con los ciclos de la luna. Con el objetivo de determinar la relación entre las capturas de langosta espinosa Panulirus Argus (Decapoda: Palinuridae) y el ciclo lunar, en la zona del Golfo de Batabanó, se correlacionaron los niveles de iluminación nocturna del ciclo lunar (INT) con la captura diaria por barco (CDB) durante siete meses lunares correspondientes al período de pesca del 2002. Los datos de captura provinieron de las tres empresas pesqueras que realizan su actividad en la zona del Golfo de Batabanó. La relación entre el ciclo lunar y las capturas de langostas fue examinada mediante varios procedimientos estadísticos. Los primeros gráficos realizados, cualitativos, mostraron que no existe relación entre las tasas de captura y el ciclo lunar. Los coeficientes de Spearman calculados, modularmente menores que 0.1 en todos los casos, demostraron cuantitativamente que no existe correlación entre las mencionadas variables. La prueba de Kruskal-Wallis detectó diferencias sólo en una de las tres áreas estudiadas. Las correlaciones cruzadas no detectaron significación en ninguna de las zonas, con coeficientes de correlación cruzada modularmente inferiores a 0.1. A diferencia de lo reportado por otros autores, las capturas de P. Argus y el ciclo lunar no presentan correlación significativa en el Golfo de Batabanó
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Influencia del ciclo lunar en la captura de langosta espinosa Panulirus Argus (Decapoda: Palinuridae) en el Golfo de Batabanó, Cuba
Vicerractoría Investigación, 2011Co-Authors: Alexander Lopeztegui, Julio A Baisre, Norberto CapetilloAbstract:Muchos procesos biológicos tales como los comportamientos reproductivos y migratorios se han asociado con los ciclos de la luna. Con el objetivo de determinar la relación entre las capturas de langosta espinosa Panulirus Argus (Decapoda: Palinuridae) y el ciclo lunar, en la zona del Golfo de Batabanó, se correlacionaron los niveles de iluminación nocturna del ciclo lunar (INT) con la captura diaria por barco (CDB) durante siete meses lunares correspondientes al período de pesca del 2002. Los datos de captura provinieron de las tres empresas pesqueras que realizan su actividad en la zona del Golfo de Batabanó. La relación entre el ciclo lunar y las capturas de langostas fue examinada mediante varios procedimientos estadísticos. Los primeros gráficos realizados, cualitativos, mostraron que no existe relación entre las tasas de captura y el ciclo lunar. Los coeficientes de Spearman calculados, modularmente menores que 0.1 en todos los casos, demostraron cuantitativamente que no existe correlación entre las mencionadas variables. La prueba de Kruskal-Wallis detectó diferencias sólo en una de las tres áreas estudiadas. Las correlaciones cruzadas no detectaron significación en ninguna de las zonas, con coeficientes de correlación cruzada modularmente inferiores a 0.1. A diferencia de lo reportado por otros autores, las capturas de P. Argus y el ciclo lunar no presentan correlación significativa en el Golfo de Batabanó.Influence of lunar cycle on catches of spiny lobster Panulirus Argus (Decapoda: Palinuridae) in the Gulf of Batabanó, Cuba. Many biological processes such as reproductive and migratory behaviours have been associated with moon cycles. In this study, the nocturnal light levels associated with lunar cycle (INT) were correlated with daily catch rate of lobster P. Argus, during seven lunar months of 2002 fishing period, to determine a possible relationship between these variables. The lobster catches were obtained from three fishing companies that develop their activities in the Gulf of Batabanó: EPICOL that fishes in Coloma area; PESCAHABANA in Batabanó area and PESCAISLA in Isla area. Daily catch per boat (CDB) was used as a measurement of daily catch variations (catch rate). The correlation was analyzed showing it in chronological graphs based on average of CDB per lunar phases, comparing lobster catch rate per lunar phases -with the Kruskal-Wallis test-. Spearman rank correlation coefficient and cross correlation techniques were also applied. Similarities between lobster catch rate and the lunar cycle were not found. Spearman rank correlation coefficient was modularly smaller than 0.1 in all cases and demonstrated quantitatively that correlation between CDB and INT does not exist. Kruskal-Wallis test detected differences only in Batabanó area but not when making the analyses for the whole Gulf of Batabanó. Finally, the cross correlations do not detected significance in any zone, as well. It is concluded that, in opposition to what other authors have reported, the catch rates of P. Argus and the lunar cycle did not show significant correlation in the Gulf of Batabanó. This trend was independent of the fishing art, which varied according to the time of the year that was analyzed. Rev. Biol. Trop. 59 (1): 207-216. Epub 2011 March 01
