The Experts below are selected from a list of 189 Experts worldwide ranked by ideXlab platform
Masahiro Fujishima - One of the best experts on this subject based on the ideXlab platform.
-
draft genome sequences of three holospora species holospora obtusa holospora undulata and holospora elegans endonuclear symbiotic bacteria of the ciliate Paramecium caudatum
Fems Microbiology Letters, 2014Co-Authors: Hideo Dohra, Masahiro Fujishima, Tomohiro Suzuki, Kenya Tanaka, Haruo SuzukiAbstract:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum ; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans . We investigate functions of orthologous core genes conserved across the three Holospora species, which may be essential for the infection and survival in the host nucleus.
-
Draft Genome Sequence of Holospora undulata Strain HU1, a Micronucleus-Specific Symbiont of the Ciliate Paramecium caudatum.
Genome announcements, 2013Co-Authors: Hideo Dohra, Haruo Suzuki, Tomohiro Suzuki, Kenya Tanaka, Masahiro FujishimaAbstract:ABSTRACT Holospora undulata is a micronucleus-specific symbiont of the ciliate Paramecium caudatum. We report here the draft genome sequence of H. undulata strain HU1. This genome information will contribute to the study of symbiosis between H. undulata and the host P. caudatum.
-
A macronuclear nucleoli-specific protein of the ciliate Paramecium caudatum
European Journal of Protistology, 1996Co-Authors: Masahiro Fujishima, Masaaki Iwamoto, Agnes K. Fok, Richard D. AllenAbstract:Summary A monoclonal antibody, mAb MA-1 raised against the macronuclei of the ciliate Paramecium caudatum, has been shown to react with macronuclear nucleoli by cryo-immunoelectron microscopy. Immunoblots of two-dimensional sodium dodecylsulfate Polyacrylamide gel electrophoresis showed that the MA-1 antigen was 48 kilodaltons in molecular weight and was the most abundant protein of the isolated macronuclei.
James M. Valles - One of the best experts on this subject based on the ideXlab platform.
-
Aligning Paramecium caudatum with Static Magnetic Fields
Biophysical journal, 2006Co-Authors: Karine Guevorkian, James M. VallesAbstract:As they negotiate their environs, unicellular organisms adjust their swimming in response to various physical fields such as temperature, chemical gradients, and electric fields. Because of the weak magnetic properties of most biological materials, however, they do not respond to the earth's magnetic field (5 x 10(-5) Tesla) except in rare cases. Here, we show that the trajectories of Paramecium caudatum align with intense static magnetic fields >3 Tesla. Otherwise straight trajectories curve in magnetic fields and eventually orient parallel or antiparallel to the applied field direction. Neutrally buoyant immobilized paramecia also align with their long axis in the direction of the field. We model this magneto-orientation as a strictly passive, nonphysiological response to a magnetic torque exerted on the diamagnetically anisotropic components of the paramecia. We have determined the average net anisotropy of the diamagnetic susceptibility, Deltachi(p), of a whole Paramecium: Deltachi(p) = (6.7+/- 0.7) x 10(-23) m(3). We show how the measured Deltachi(p) compares to the anisotropy of the diamagnetic susceptibilities of the components in the cell. We suggest that magnetic fields can be exploited as a novel, noninvasive, quantitative means to manipulate swimming populations of unicellular organisms.
Karine Guevorkian - One of the best experts on this subject based on the ideXlab platform.
-
Aligning Paramecium caudatum with Static Magnetic Fields
Biophysical journal, 2006Co-Authors: Karine Guevorkian, James M. VallesAbstract:As they negotiate their environs, unicellular organisms adjust their swimming in response to various physical fields such as temperature, chemical gradients, and electric fields. Because of the weak magnetic properties of most biological materials, however, they do not respond to the earth's magnetic field (5 x 10(-5) Tesla) except in rare cases. Here, we show that the trajectories of Paramecium caudatum align with intense static magnetic fields >3 Tesla. Otherwise straight trajectories curve in magnetic fields and eventually orient parallel or antiparallel to the applied field direction. Neutrally buoyant immobilized paramecia also align with their long axis in the direction of the field. We model this magneto-orientation as a strictly passive, nonphysiological response to a magnetic torque exerted on the diamagnetically anisotropic components of the paramecia. We have determined the average net anisotropy of the diamagnetic susceptibility, Deltachi(p), of a whole Paramecium: Deltachi(p) = (6.7+/- 0.7) x 10(-23) m(3). We show how the measured Deltachi(p) compares to the anisotropy of the diamagnetic susceptibilities of the components in the cell. We suggest that magnetic fields can be exploited as a novel, noninvasive, quantitative means to manipulate swimming populations of unicellular organisms.
Haruo Suzuki - One of the best experts on this subject based on the ideXlab platform.
-
draft genome sequences of three holospora species holospora obtusa holospora undulata and holospora elegans endonuclear symbiotic bacteria of the ciliate Paramecium caudatum
Fems Microbiology Letters, 2014Co-Authors: Hideo Dohra, Masahiro Fujishima, Tomohiro Suzuki, Kenya Tanaka, Haruo SuzukiAbstract:We present draft genome sequences of three Holospora species, hosted by the ciliate Paramecium caudatum ; that is, the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata and H. elegans . We investigate functions of orthologous core genes conserved across the three Holospora species, which may be essential for the infection and survival in the host nucleus.
-
Draft Genome Sequence of Holospora undulata Strain HU1, a Micronucleus-Specific Symbiont of the Ciliate Paramecium caudatum.
Genome announcements, 2013Co-Authors: Hideo Dohra, Haruo Suzuki, Tomohiro Suzuki, Kenya Tanaka, Masahiro FujishimaAbstract:ABSTRACT Holospora undulata is a micronucleus-specific symbiont of the ciliate Paramecium caudatum. We report here the draft genome sequence of H. undulata strain HU1. This genome information will contribute to the study of symbiosis between H. undulata and the host P. caudatum.
O. V. Karpukhina - One of the best experts on this subject based on the ideXlab platform.
-
influence of a medium s ph on Paramecium caudatum cells under exposure to the low temperature atmospheric pressure plasma
Russian Journal of Physical Chemistry B, 2020Co-Authors: I. G. Kalinina, A. N. Inozemtsev, O. V. Karpukhina, V. P. Savinov, V. G. Yakunin, V. A. Ryabyi, V. B. IvanovAbstract:It is shown that when exposed to low-temperature atmospheric pressure plasma (LTAPP) on a single-cell culture of Paramecium caudatum as a result of changes in the external conditions (ionic composition of the solution), the nature of the movement changes and the number of viable cells decreases. The viability of Paramecium caudatum cells depends on the time of exposure to the plasma and is correlated with the changes in the pH of the medium. The kinetic features of the processes discovered in the work are analyzed: the aftereffect of exposure to the plasma and the stepwise nature of the cells’ death.
-
Influence of a Medium’s pH on Paramecium caudatum Cells under Exposure to the Low Temperature Atmospheric Pressure Plasma
Russian Journal of Physical Chemistry B, 2020Co-Authors: I. G. Kalinina, A. N. Inozemtsev, O. V. Karpukhina, V. P. Savinov, V. G. Yakunin, V. A. Ryabyi, V. B. IvanovAbstract:It is shown that when exposed to low-temperature atmospheric pressure plasma (LTAPP) on a single-cell culture of Paramecium caudatum as a result of changes in the external conditions (ionic composition of the solution), the nature of the movement changes and the number of viable cells decreases. The viability of Paramecium caudatum cells depends on the time of exposure to the plasma and is correlated with the changes in the pH of the medium. The kinetic features of the processes discovered in the work are analyzed: the aftereffect of exposure to the plasma and the stepwise nature of the cells’ death.
-
Effect of low temperature plasma of atmospheric pressure on single-cell model organisms of ciliate Paramecium caudatum
Journal of Physics: Conference Series, 2019Co-Authors: A. N. Inozemtsev, O. V. Karpukhina, I. V. Melikhov, V. A. Riaby, V. P. Savinov, V. Y. Timoshenko, V. G. YakuninAbstract:We studied low-temperature plasma effects on Paramecium caudatum using the original electric arc plasmatron. The decrease in the number of experimental cells has been shown, which may indicate membrane destructive pathology due to oxidative stress induced by the plasma impact. It is concluded that reactions at the population level of Paramecium caudatum are effective indices of primary information about plasma radiation cytotoxicity.
-
Effect of long-wavelength coherent radiation on a biological object (unicellular organism Paramecium caudatum) in presence of silicon nanoparticles
Journal of Physics: Conference Series, 2019Co-Authors: A. F. Alykova, O. V. Karpukhina, N. V. Karpov, V A Oleshenko, O. M. Alykova, V V Bezotosniy, S. M. Klimentov, V. Yu. Timoshenko, Irina N. ZavestovskayaAbstract:We report an effect of continuous and pulsed near infrared (808 nm) laser radiation on cells (Paramecium caudatum) incubated with silicon (Si) nanoparticles (NPs), which were obtained by laser ablation of crystalline Si wafer in water. The data obtained indicate the possibility of enhancing the destructive effect on cells by using pulsed laser irradiation and Si NPs.
