The Experts below are selected from a list of 291 Experts worldwide ranked by ideXlab platform
Jaeyoung Choi - One of the best experts on this subject based on the ideXlab platform.
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complementary effect of Phloxine b on the insecticidal efficacy of isaria fumosorosea sfp 198 wettable powder against greenhouse whitefly trialeurodes vaporariorum west
Pest Management Science, 2010Co-Authors: Yeon Ho Je, Jaeyoung ChoiAbstract:BACKGROUND: Insecticidal activities of five photoactive dyes against greenhouse whitefly (GWF), Trialeurodes vaporariorum West., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, Isaria fumosorosea SFP-198 wettable powder (WP). Azorubine, Eosin B, Erythrosine, Brilliant Green and Phloxine B were used in this work, accompanying pyriproxyfen emulsifiable concentrate (EC) as a commercial standard for comparison. RESULTS: Phloxine B had the highest control efficacy in glasshouse conditions. The most suitable dose of Phloxine B was determined as 0.005 g L−1, given the dosage-dependent control efficacy and phytotoxicity of the dye, and its influence on the germination of SFP-198 conidia. In field conditions, SFP-198 WP + Phloxine B (2 + 0.005 g L−1; tank mix) showed 89.1 and 95.3% control efficacy 7 and 14 days post-application, which was significantly higher than the efficacy of SFP-198 WP alone (43.5 and 64.0%), Phloxine B (47.5 and 30.7%) or pyriproxyfen EC (67.7 and 80.2%). CONCLUSION: Phloxine B cooperates with SFP-198 WP complementarily, possibly in the order of killing action (early: Phloxine B; late: SFP-198 WP). The dye can be incorporated into SFP-198 WP to improve its efficacy and applied to other Hypocrelean entomopathogenic fungal products. Copyright © 2010 Society of Chemical Industry
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Complementary effect of Phloxine B on the insecticidal efficacy of Isaria fumosorosea SFP‐198 wettable powder against greenhouse whitefly, Trialeurodes vaporariorum West.
Pest Management Science, 2010Co-Authors: Yeon Ho Je, Jaeyoung ChoiAbstract:BACKGROUND: Insecticidal activities of five photoactive dyes against greenhouse whitefly (GWF), Trialeurodes vaporariorum West., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, Isaria fumosorosea SFP-198 wettable powder (WP). Azorubine, Eosin B, Erythrosine, Brilliant Green and Phloxine B were used in this work, accompanying pyriproxyfen emulsifiable concentrate (EC) as a commercial standard for comparison. RESULTS: Phloxine B had the highest control efficacy in glasshouse conditions. The most suitable dose of Phloxine B was determined as 0.005 g L−1, given the dosage-dependent control efficacy and phytotoxicity of the dye, and its influence on the germination of SFP-198 conidia. In field conditions, SFP-198 WP + Phloxine B (2 + 0.005 g L−1; tank mix) showed 89.1 and 95.3% control efficacy 7 and 14 days post-application, which was significantly higher than the efficacy of SFP-198 WP alone (43.5 and 64.0%), Phloxine B (47.5 and 30.7%) or pyriproxyfen EC (67.7 and 80.2%). CONCLUSION: Phloxine B cooperates with SFP-198 WP complementarily, possibly in the order of killing action (early: Phloxine B; late: SFP-198 WP). The dye can be incorporated into SFP-198 WP to improve its efficacy and applied to other Hypocrelean entomopathogenic fungal products. Copyright © 2010 Society of Chemical Industry
Reuven Rasooly - One of the best experts on this subject based on the ideXlab platform.
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Phloxine b a versatile bacterial stain
Fems Immunology and Medical Microbiology, 2007Co-Authors: Reuven RasoolyAbstract:The data presented suggest that Phloxine B, a color additive for food, drugs, and cosmetics has a potential use as a nontoxic, faster (<2 min), inexpensive (350 tests for <1 cent material) and simpler to use alternative to Gram staining. Using Phloxine B staining it was possible to differentiate among Gram-negative and Gram-positive bacteria by visual determination under normal room lighting, light microscopy, fluorescence microscopy and confocal microscopy. This work demonstrated that Phloxine B can be used as a differential versatile bacterial stain and establishes a correlation between the staining properties of the dye and its bactericidal effect.
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Phloxine B, a versatile bacterial stain
Fems Immunology and Medical Microbiology, 2007Co-Authors: Reuven RasoolyAbstract:The data presented suggest that Phloxine B, a color additive for food, drugs, and cosmetics has a potential use as a nontoxic, faster (
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Expanding the bactericidal action of the food color additive Phloxine B to gram-negative bacteria
FEMS immunology and medical microbiology, 2005Co-Authors: Reuven RasoolyAbstract:Phloxine B (D&C red no. 28) is a color additive for food, drugs, and cosmetics. It has been previously shown to have anti-Staphylococcus aureus activities. In this work, the effect of Phloxine B on various gram-negative bacteria and other gram-positive bacteria including Bacillus cereus, Bacillus thuringiensis, Bacillus mycoides, Bacillus subtilis, Bacillus aureus, Salmonella, Escherichia coli and Shigella was studied, along with the mechanism of anti-microbial activity. In the presence of fluorescent light, the viable count for gram-positive bacteria, (Bacillus spp. and S. aureus) decreased in a dose and time dependent manner when incubated with Phloxine B. The viability of gram-positive bacteria was reduced by 99.99% in 40 min, while there was no effect on gram-negative bacteria (Salmonella choleraesuis, E. coli and Shigella flexneri). However, the use of ethylenediaminetetraacetic acid (EDTA) expands the spectrum of activity for Phloxine B to include gram-negative bacteria. EDTA increased membrane-permeability by releasing lipopolysaccharide. Overall, in an Agar diffusion test the light-dependent bactericidal activity of 1 μg of Phloxine B had a potency of 0.64 units of chloramphenicol and 0.5 units of tetracycline when tested on B. cereus, and had a potency of 0.7 units of chloramphenicol and 0.2 units of tetracycline when tested on S. aureus. The data suggest that the dye may have some potential anti-microbial applications.
Yeon Ho Je - One of the best experts on this subject based on the ideXlab platform.
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complementary effect of Phloxine b on the insecticidal efficacy of isaria fumosorosea sfp 198 wettable powder against greenhouse whitefly trialeurodes vaporariorum west
Pest Management Science, 2010Co-Authors: Yeon Ho Je, Jaeyoung ChoiAbstract:BACKGROUND: Insecticidal activities of five photoactive dyes against greenhouse whitefly (GWF), Trialeurodes vaporariorum West., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, Isaria fumosorosea SFP-198 wettable powder (WP). Azorubine, Eosin B, Erythrosine, Brilliant Green and Phloxine B were used in this work, accompanying pyriproxyfen emulsifiable concentrate (EC) as a commercial standard for comparison. RESULTS: Phloxine B had the highest control efficacy in glasshouse conditions. The most suitable dose of Phloxine B was determined as 0.005 g L−1, given the dosage-dependent control efficacy and phytotoxicity of the dye, and its influence on the germination of SFP-198 conidia. In field conditions, SFP-198 WP + Phloxine B (2 + 0.005 g L−1; tank mix) showed 89.1 and 95.3% control efficacy 7 and 14 days post-application, which was significantly higher than the efficacy of SFP-198 WP alone (43.5 and 64.0%), Phloxine B (47.5 and 30.7%) or pyriproxyfen EC (67.7 and 80.2%). CONCLUSION: Phloxine B cooperates with SFP-198 WP complementarily, possibly in the order of killing action (early: Phloxine B; late: SFP-198 WP). The dye can be incorporated into SFP-198 WP to improve its efficacy and applied to other Hypocrelean entomopathogenic fungal products. Copyright © 2010 Society of Chemical Industry
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Complementary effect of Phloxine B on the insecticidal efficacy of Isaria fumosorosea SFP‐198 wettable powder against greenhouse whitefly, Trialeurodes vaporariorum West.
Pest Management Science, 2010Co-Authors: Yeon Ho Je, Jaeyoung ChoiAbstract:BACKGROUND: Insecticidal activities of five photoactive dyes against greenhouse whitefly (GWF), Trialeurodes vaporariorum West., in tomatoes were investigated to improve the control efficacy of an entomopathogenic fungal product, Isaria fumosorosea SFP-198 wettable powder (WP). Azorubine, Eosin B, Erythrosine, Brilliant Green and Phloxine B were used in this work, accompanying pyriproxyfen emulsifiable concentrate (EC) as a commercial standard for comparison. RESULTS: Phloxine B had the highest control efficacy in glasshouse conditions. The most suitable dose of Phloxine B was determined as 0.005 g L−1, given the dosage-dependent control efficacy and phytotoxicity of the dye, and its influence on the germination of SFP-198 conidia. In field conditions, SFP-198 WP + Phloxine B (2 + 0.005 g L−1; tank mix) showed 89.1 and 95.3% control efficacy 7 and 14 days post-application, which was significantly higher than the efficacy of SFP-198 WP alone (43.5 and 64.0%), Phloxine B (47.5 and 30.7%) or pyriproxyfen EC (67.7 and 80.2%). CONCLUSION: Phloxine B cooperates with SFP-198 WP complementarily, possibly in the order of killing action (early: Phloxine B; late: SFP-198 WP). The dye can be incorporated into SFP-198 WP to improve its efficacy and applied to other Hypocrelean entomopathogenic fungal products. Copyright © 2010 Society of Chemical Industry
Jocelyn P. Alcantara-licudine - One of the best experts on this subject based on the ideXlab platform.
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Method for Determination of Xanthene Dyes in Guava Fruits and Its Application in a Field Dissipation Study
Journal of AOAC International, 2000Co-Authors: Jocelyn P. Alcantara-licudine, Grant T Mcquate, Ngoc Lan Bui, Steven L PeckAbstract:Xanthene dyes, i.e., Phloxine B and uranine or Phloxine B alone, are phototoxic to tephritid fruit flies infesting guava fruits. An analytical method was developed for determination of residues of these dyes used in bait solutions for suppression of the tephritid fruit fly population in guava fruits. The procedure involved solvent extraction, anion-exchange cleanup, and determination by liquid chromatography or capillary zone electrophoresis. The dyes were extracted from 50 g guava fruit at 45 degrees degrees with 400 mL methanol-acetonitrile (1 + 1) and 5 g magnesium oxide added as an alkaline and clarifying agent. The guava extract was adjusted to pH 8.5 and subjected to an amino column cleanup. Average recoveries of xanthene dyes added to guava purees ranged from 77 to 99% for Phloxine B and from 79 to 102% for uranine at spiking levels of 0.05-1.00 microg/g. The method was applied to the determination of Phloxine B residues in guava fruits collected from a dye-sprayed orchard. After Phloxine B was applied at a rate of 62.5 g/ha for 14 weekly sprayings, it was found on guava fruits at an average concentration of 111 +/- 18 ng/g 4 h after the llth spraying. The concentration of Phloxine B was 426 +/- 94 ng/g in selected fruits with high deposits of the dye 4 h after spraying. Average concentrations of Phloxine B 5 days after the 7th and 14th sprayings were 29 +/- 7 and 19 +/- 8 ng/g, respectively.
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Environmental analysis and fate of photoactive xanthene insecticides
1999Co-Authors: Jocelyn P. Alcantara-licudineAbstract:Phloxine B and uranine are xanthene dyes used as coloring additives in drugs and cosmetics in the U.S. A mixture of Phloxine B and uranine or Phloxine B alone mixed with protein baits has been shown to be a safe and effective insecticide for suppression of fruit flies and could replace malathion. Currently, malathion is the major insecticide being used to control fruit fly pests, but it has received strong public opposition because of perceived public health and environmental concerns. Methods were developed for the analysis of xanthene dyes in coffee, soil, sediment and water. Xanthene dyes in soil were quantitatively recovered by supercritical fluid extraction. Phloxine B and uranine were effectively extracted with a mixture of solvents from coffee cherries, green beans and roasted beans. Phloxine B and uranine in water were quantitatively extracted with solid phase extraction. A capillary zone electrophoretic method was developed to measure Phloxine B and uranine in sample extracts. A high-performance liquid chromatographic method was also developed for the analysis of xanthene dyes. These methods can detect Phloxine B and uranine at 50 parts per billion (ppb) in coffee cherries, 8 ppb in soil, and 10 ppb in water. Xanthene dyes were rapidly broken down in laboratory and field conditions. Half-life (t 0.5 ) of Phloxine B was less than one week on plants and soil in coffee fields. Uranine underwent faster degradation than Phloxine B. In laboratory conditions, Phloxine B t 0.5 was less than one-half hour in water under sunlight. Photolysis rate of Phloxine B is influenced by various factors including salts in water, water pH, and light intensity. Soil organic carbon/water partition coefficients (Koc) were 145-189 for Phloxine B and 69-89 for uranine. Rapid degradation and relative strong adsorption to soils indicated limited leaching potential into groundwater. However, their effects and degradation in the environment particularly to nontarget organisms such as aquatic species need further investigations.
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Analysis of Phloxine B and Uranine in Coffee by High-Performance Liquid Chromatography and Capillary Zone Electrophoresis after Solid Phase Extraction Cleanup
Journal of Agricultural and Food Chemistry, 1998Co-Authors: Jocelyn P. Alcantara-licudine, Ngoc Lan Bui, Michael K. KawateAbstract:A method was developed for the analysis of Phloxine B and uranine, photoactive dyes being evaluated as fruit fly toxicants, in coffee cherries and green and roasted beans. The analytes were measure...
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Determination of Phloxine B and uranine in water by capillary zone electrophoresis
Journal of chromatographic science, 1997Co-Authors: Jocelyn P. Alcantara-licudineAbstract:Phloxine B and uranine are color additives in drugs and cosmetics as well as potential photoactive insecticides. A capillary zone electrophoretic (CZE) method is developed to determine Phloxine B and uranine in water. A fused-silica capillary (67cm, 75-μm i.d.) and borate buffer are used. Migration of Phloxine B and uranine increases slightly as the pH of the running buffer increases between the range of 8-9. Although there are only slight effects of ionic strength on the analyte migration in the range of 0-20mM NaCl in the running buffer, the migration of Phloxine B and uranine increases as the percentage of methanol in the samples increases. Methanol shows little effect on the quantitation of Phloxine B and uranine. The CZE procedure is applied to determine Phloxine B and uranine fortified in tap and stream water samples. Solid- phase extraction is employed to recover the analytes spiked in the water samples. Recoveries range from 87-112% for Phloxine B spiked at 10-200 ppb in the tap and stream water. Uranine recoveries are 86-91% at fortification levels of 10-50 ppb in the water samples.
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Method for the analysis of Phloxine B, uranine, and related xanthene dyes in soil using supercritical fluid extraction and high-performance liquid chromatography
Journal of Agricultural and Food Chemistry, 1997Co-Authors: Jocelyn P. Alcantara-licudine, And Michael K. KawateAbstract:The use of supercritical fluid (SF) carbon dioxide (CO2) modified by organic solvents and inorganic salts or chelating reagents was investigated for the extraction of the xanthene dyes Phloxine B and uranine from soil. Methanol (MeOH), n-butylamine (n-BA), and a chelating agent, ethylenediaminetetraacetic acid tetrasodium salt (Na4EDTA), were the most effective modifiers of SF CO2 for quantitative recoveries of Phloxine B and uranine in soils with 10−20% moisture at 60 °C/476 atm and 60 °C/272 atm, respectively. At these supercritical fluid extraction (SFE) conditions, recoveries of related xanthene dyes (i.e., 2‘,7‘-dichlorofluorescein, 4,5,6,7-tetrachlorofluorescein, eosin Y lactone, erythrosin B, and rose bengal) fortified at 25 μg/g in Hawaiian soils ranged from 65 to 93%. Good separation of a mixture of these dyes was achieved by HPLC. A mixture of MeOH, n-BA, and sodium hexametaphosphate [(NaPO3)6] was effective for conventional solvent extraction of Phloxine B and uranine from fortified soils. Howe...
Stephan Kamrad - One of the best experts on this subject based on the ideXlab platform.
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pyphe a python toolbox for assessing microbial growth and cell viability in high throughput colony screens
eLife, 2020Co-Authors: Stephan Kamrad, Maria Rodriguezlopez, Cristina Cotobal, Clara Correiamelo, Markus Ralser, Jurg BahlerAbstract:Microbial fitness screens are a key technique in functional genomics. We present an all-in-one solution, pyphe, for automating and improving data analysis pipelines associated with large-scale fitness screens, including image acquisition and quantification, data normalisation, and statistical analysis. Pyphe is versatile and processes fitness data from colony sizes, viability scores from Phloxine B staining or colony growth curves, all obtained with inexpensive transilluminating flatbed scanners. We apply pyphe to show that the fitness information contained in late endpoint measurements of colony sizes is similar to maximum growth slopes from time series. We phenotype gene-deletion strains of fission yeast in 59,350 individual fitness assays in 70 conditions, revealing that colony size and viability provide complementary, independent information. Viability scores obtained from quantifying the redness of Phloxine-stained colonies accurately reflect the fraction of live cells within colonies. Pyphe is user-friendly, open-source and fully documented, illustrated by applications to diverse fitness analysis scenarios.