The Experts below are selected from a list of 112176 Experts worldwide ranked by ideXlab platform
Eun Kyung Oh - One of the best experts on this subject based on the ideXlab platform.
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abstract 2316 anti tumor effects of on the combination of photodynamic therapy with arsenic compound in tc 1 cells implanted c57bl 6 mice
Cancer Research, 2009Co-Authors: Eun Kyung OhAbstract:AACR Annual Meeting-- Apr 18-22, 2009; Denver, CO Objective: We studied the effects of As4O6 as adjuvant on photodynamic therapy of TC-1 cells in order to provide higher efficiency and explored the mechanisms underlying these effects. As4O6 is considered to have anticancer activity via several biological actions such as free radical producing and inhibition of VEGF expression. Methods and Materials : The present study was consisted of two part. In vitro experiments, cell proliferation and morphology was determined by MTT assay, when TC-1 cells, which overexpressed the HPV16-E6/E7, were exposed to either photodynamic therapy or As4O6 alone, or vehicle alone. Also, quantitative PCR array was performed to assay the signal transduction regulation. Additionally, this study was supported by the finding that combination of photodynamic therapy and As4O6 shows a inhibition effect of tumor growth in C57BL/6 mice with TC-1 cells xenographs in vivo . Results and conclusions; Cell proliferation was first estimated using an MTT assay after TC-1 cells were treated with either Radachlorin or As4O6 at various concentrations. Radachlorin and As4O6 significantly inhibited TC-1 cell proliferation in a dose-dependent manner (P<0.05). Antiproliferative effect of combination treatment was significantly higher than those of TC-1 cells treated with either photodynamic therapy or As4O6 (62.4 and 52.5% decrease, respectively, compared to photodynamic therapy or As4O6 alone, P <0.05). In addition, cell proliferation in combination of photodynamic therapy and As4O6 treatment significantly decreased by 77.4% compared to vehicle-only treated TC-1 cells (P<0.05). This inhibition in turn led to induce the apoptotic morphological features such as cell shrinkage and blebbing. Cell survival pathway (Naip1, Tert and Aip1) and p53-dependent pathway (Bax, p21Cip1, Fas, Gadd45, IGFBP-3 and Mdm-2) were markedly increased by combination treatment of photodynamic therapy and As4O6. Besides, the immunology response NEAT pathway (Ly-12, CD178 and IL-2) also modulated after combination treatment of photodynamic therapy and As4O6. This combination effect apparently shows a same pattern in vivo model. These findings suggest the benefit of the combination treatment of photodynamic therapy and As4O6 for the inhibition of cervical cancer growth Citation Information: In: Proc Am Assoc Cancer Res; 2009 Apr 18-22; Denver, CO. Philadelphia (PA): AACR; 2009. Abstract nr 2316.
Ralph Devere W White - One of the best experts on this subject based on the ideXlab platform.
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novel theranostic nanoporphyrins for photodynamic diagnosis and trimodal therapy for bladder cancer
Biomaterials, 2016Co-Authors: Yuanpei Li, Jui Lin Chen, Hongyong Zhang, Hua Zhang, Katherine W Ferrara, Sebastian Wachsmannhogiu, Tianhong Li, Susan D Airhart, Ralph Devere W WhiteAbstract:The overall prognosis of bladder cancer has not been improved over the last 30 years and therefore, there is a great medical need to develop novel diagnosis and therapy approaches for bladder cancer. We developed a multifunctional nanoporphyrin platform that was coated with a bladder cancer-specific ligand named PLZ4. PLZ4-nanoporphyrin (PNP) integrates photodynamic diagnosis, image-guided photodynamic therapy, photothermal therapy and targeted chemotherapy in a single procedure. PNPs are spherical, relatively small (around 23 nm), and have the ability to preferably emit fluorescence/heat/reactive oxygen species upon illumination with near infrared light. Doxorubicin (DOX) loaded PNPs possess slower drug release and dramatically longer systemic circulation time compared to free DOX. The fluorescence signal of PNPs efficiently and selectively increased in bladder cancer cells but not normal urothelial cells in vitro and in an orthotopic patient derived bladder cancer xenograft (PDX) models, indicating their great potential for photodynamic diagnosis. Photodynamic therapy with PNPs was significantly more potent than 5-aminolevulinic acid, and eliminated orthotopic PDX bladder cancers after intravesical treatment. Image-guided photodynamic and photothermal therapies synergized with targeted chemotherapy of DOX and significantly prolonged overall survival of mice carrying PDXs. In conclusion, this uniquely engineered targeting PNP selectively targeted tumor cells for photodynamic diagnosis, and served as effective triple-modality (photodynamic/photothermal/chemo) therapeutic agents against bladder cancers. This platform can be easily adapted to individualized medicine in a clinical setting and has tremendous potential to improve the management of bladder cancer in the clinic.
Zhenzhong Zhang - One of the best experts on this subject based on the ideXlab platform.
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Photodynamic effect of functionalized single-walled carbon nanotubes: a potential sensitizer for photodynamic therapy
Nanoscale, 2014Co-Authors: Lei Wang, Jinjin Shi, Liu Ruiyuan, Yan Liu, Zhang Jing, Yu Xiaoyuan, Jun Gao, Chaofeng Zhang, Zhenzhong ZhangAbstract:Single-walled carbon nanotubes (SWNTs) possess unique physical and chemical properties, which make them very attractive for a wide range of applications. In particular, SWNTs and their composites have shown a great potential for photodynamic therapy (PDT). SWNTs have usually been used for photothermal therapy; herein, the photodynamic effect of two functionalized SWNTs are detected under visible light illumination in vitro and in vivo. The results indicated that the photodynamic effect is not entirely dependent on illumination time, but also on the modification method of the SWNTs. The ability of SWNTs complexes to combine with photodynamic therapy significantly improved the therapeutic efficacy of cancer treatment, and the combined treatment demonstrated a synergistic effect. These findings suggest that the SWNTs composite has great potential as sensitizer for PDT.
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the application of hyaluronic acid derivatized carbon nanotubes in hematoporphyrin monomethyl ether based photodynamic therapy for in vivo and in vitro cancer treatment
International Journal of Nanomedicine, 2013Co-Authors: Lei Wang, Jing Zhang, Lulu Li, Xiaoyuan Yu, Zhenzhong ZhangAbstract:Carbon nanotubes (CNTs) have shown great potential in both photothermal therapy and drug delivery. In this study, a CNT derivative, hyaluronic acid-derivatized CNTs (HA-CNTs) with high aqueous solubility, neutral pH, and tumor-targeting activity, were synthesized and characterized, and then a new photodynamic therapy agent, hematoporphyrin monomethyl ether (HMME), was adsorbed onto the functionalized CNTs to develop HMME-HA-CNTs. Tumor growth inhibition was investigated both in vivo and in vitro by a combination of photothermal therapy and photodynamic therapy using HMME-HA-CNTs. The ability of HMME-HA-CNT nanoparticles to combine local specific photodynamic therapy with external near-infrared photothermal therapy significantly improved the therapeutic efficacy of cancer treatment. Compared with photodynamic therapy or photothermal therapy alone, the combined treatment demonstrated a synergistic effect, resulting in higher therapeutic efficacy without obvious toxic effects to normal organs. Overall, it was demonstrated that HMME-HA-CNTs could be successfully applied to photodynamic therapy and photothermal therapy simultaneously in future tumor therapy.
Yuanpei Li - One of the best experts on this subject based on the ideXlab platform.
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novel theranostic nanoporphyrins for photodynamic diagnosis and trimodal therapy for bladder cancer
Biomaterials, 2016Co-Authors: Yuanpei Li, Jui Lin Chen, Hongyong Zhang, Hua Zhang, Katherine W Ferrara, Sebastian Wachsmannhogiu, Tianhong Li, Susan D Airhart, Ralph Devere W WhiteAbstract:The overall prognosis of bladder cancer has not been improved over the last 30 years and therefore, there is a great medical need to develop novel diagnosis and therapy approaches for bladder cancer. We developed a multifunctional nanoporphyrin platform that was coated with a bladder cancer-specific ligand named PLZ4. PLZ4-nanoporphyrin (PNP) integrates photodynamic diagnosis, image-guided photodynamic therapy, photothermal therapy and targeted chemotherapy in a single procedure. PNPs are spherical, relatively small (around 23 nm), and have the ability to preferably emit fluorescence/heat/reactive oxygen species upon illumination with near infrared light. Doxorubicin (DOX) loaded PNPs possess slower drug release and dramatically longer systemic circulation time compared to free DOX. The fluorescence signal of PNPs efficiently and selectively increased in bladder cancer cells but not normal urothelial cells in vitro and in an orthotopic patient derived bladder cancer xenograft (PDX) models, indicating their great potential for photodynamic diagnosis. Photodynamic therapy with PNPs was significantly more potent than 5-aminolevulinic acid, and eliminated orthotopic PDX bladder cancers after intravesical treatment. Image-guided photodynamic and photothermal therapies synergized with targeted chemotherapy of DOX and significantly prolonged overall survival of mice carrying PDXs. In conclusion, this uniquely engineered targeting PNP selectively targeted tumor cells for photodynamic diagnosis, and served as effective triple-modality (photodynamic/photothermal/chemo) therapeutic agents against bladder cancers. This platform can be easily adapted to individualized medicine in a clinical setting and has tremendous potential to improve the management of bladder cancer in the clinic.
Delmar S Larsen - One of the best experts on this subject based on the ideXlab platform.
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Reverse Photodynamics of the Noncanonical Red/Green NpR3784 Cyanobacteriochrome from Nostoc punctiforme
2019Co-Authors: Julia S. Kirpich, Sean M Gottlieb, Peter W Kim, Chewei Chang, Shelley S Martin, Clark J Lagarias, Delmar S LarsenAbstract:In the companion paper (10.1021/acs.biochem.8b01274), we examined the forward Pr Photodynamics of NpR3784 (UniProtKB B2J457), a representative member of a noncanonical red/green (R/G) cyanobacteriochrome (CBCR) subfamily. Here the reverse Pg → Pr Photodynamics of NpR3784 was studied by broadband transient absorption pump–probe spectroscopy. Primary (100 fs to 10 ns) and secondary (10 ns to 1 ms) Photodynamics were characterized over nine decades of time, which also were complemented with temperature-jump cryokinetics measurements. In contrast with canonical R/G CBCRs, the NpR3784 reverse photoconversion yielded two spectrally distinct primary photoproducts, Lumi-Go and Lumi-Gr, which decay on different time scales. The two primary photoproducts of NpR3784 equilibrate on the 40 ns time scale and subsequently propagate as a single intermediate population into Pr. Such heterogeneity could arise from differences in the direction of D-ring rotation, in chromophore protonation or hydrogen bonding, or in the mobility of protein residues or of solvent water nearby the chromophore or some combination therein. We conclude that the atypical Photodynamics of NpR3784 reflects chromophore–protein interactions that differ from those present in the canonical R/G CBCR family
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primary and secondary Photodynamics of the violet orange dual cysteine npf2164g3 cyanobacteriochrome domain from nostoc punctiforme
Biochemistry, 2014Co-Authors: Sean M Gottlieb, Peter W Kim, Scott C Corley, Dorte Madsen, Samuel J Hanke, Chewei Chang, Nathan C Rockwell, Shelley S Martin, Clark J Lagarias, Delmar S LarsenAbstract:Cyanobacteriochromes (CBCRs) are cyanobacterial photoreceptors distantly related to phytochromes. Like phytochromes, CBCRs photointerconvert between two photostates that accompany photoisomerization of their bilin chromophores. While phytochromes typically exhibit red/far-red photocycles, CBCR photocycles are much more diverse, spanning the near-ultraviolet and the entire visible region. All CBCRs described to date have a conserved Cys residue covalently attached to the linear tetrapyrrole (bilin) chromophore; two CBCR subfamilies also exploit a second thioether linkage to the chromophore for detection of near-ultraviolet to blue light. Here, we present the photodynamic analysis of the insert-Cys CBCR NpF2164g3, a representative of the second class of two-cysteine CBCRs. Using broadband transient absorption pump–probe spectroscopy, we characterize the primary (100 fs to 10 ns) and secondary (10 ns to 1 ms) Photodynamics in both directions, examining Photodynamics over nine decades of time. Primary isomeri...
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modulating lov domain Photodynamics with a residue alteration outside the chromophore binding site
Biochemistry, 2011Co-Authors: Sanghun Song, Peter L Freddolino, Abigail I Nash, Elizabeth C Carroll, Klaus Schulten, Kevin H Gardner, Delmar S LarsenAbstract:Phototropins, a class of light-activated protein kinases, are essential for several blue light responses in plants and algae, including phototropism. These proteins contain two internal light, oxygen, and voltage sensitive (LOV) domains, which bind flavin chromophores and undergo a reversible photochemical formation of a cysteinyl-flavin adduct as part of the light sensing process. While the photodynamic properties of such photosensory domains are dictated by interactions between the chromophore and surrounding protein, more distant residues can play a significant role as well. Here we explore the role of the Phe434 residue in the photosensory response of the second LOV domain of Avena sativa phototropin 1 (AsLOV2), a model photochemical system for these LOV domains. Phe434 lies over 6 A from the FMN chromophore in AsLOV2; nevertheless, a F434Y point mutation is likely to change several structural features of the chromophore binding site, as we demonstrate using molecular dynamics simulations. Transient absorption signals spanning 15 decades in time were compared for wildtype AsLOV2 and the F434Y mutant, showing that the latter has significantly altered Photodynamics including (i) a faster intersystem crossing leading to triplet formation on a nanosecond time scale, (ii) biphasic formation of adduct state kinetics on the microsecond time scale, and (iii) greatly accelerated ground-state recovery kinetics on a second time scale. We present mechanistic models that link these spectroscopic differences to changes in the configuration of the critical cysteine residue and in the chromophore’s accessibility to solvent and oxygen according to MD trajectories and purging experiments. Taken together, these results demonstrate the importance of residues outside the chromophore-binding pocket in modulating LOV domain Photodynamics.