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Anne Willems - One of the best experts on this subject based on the ideXlab platform.
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Phyllobacterium zundukense sp. nov., a novel species of rhizobia isolated from root nodules of the legume species Oxytropis triphylla (Pall.) Pers.
International journal of systematic and evolutionary microbiology, 2018Co-Authors: Vera I. Safronova, Anne Willems, Andrey A. Belimov, Anna L. Sazanova, Janna P. Popova, Evgeny E. Andronov, Alla V. Verkhozina, Irina G. Kuznetsova, Elizaveta R. Chirak, Igor A. TikhonovichAbstract:Gram-negative strains Tri-36, Tri-38, Tri-48T and Tri-53 were isolated from root nodules of the relict legume Oxytropis triphylla (Pall.) Pers. originating from Zunduk Cape (Baikal Lake region, Russia). 16S rRNA gene sequencing showed that the novel isolates were phylogenetically closest to the type strains Phyllobacterium sophorae LMG 27899T, Phyllobacterium brassicacearum LMG 22836T, Phyllobacterium endophyticum LMG 26470T and Phyllobacterium bourgognense LMG 22837 T while similarity levels between the isolates and the most closely related strain P. endophyticum LMG 26470T were 98.8–99.5 %. The recA and glnII genes of the isolates showed highest sequence similarities with P. sophorae LMG 27899T (95.4 and 89.5 %, respectively) and P. brassicacearum LMG 22836T (91.4 and 85.1 %, respectively). Comparative analysis of phenotypic properties between the novel isolates and the closest reference strains P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T was performed using a microassay system. Average nucleotide identities between the whole genome sequences of the isolates Tri-38 and Tri-48T and P. sophorae LMG 27899T, P. brassicacearum LMG 22836T and P. endophyticum LMG 26470T ranged from 79.23 % for P. endophyticum LMG 26470T to 85.74 % for P. sophorae LMG 27899T. The common nodABC genes required for legume nodulation were absent from strains Tri-38 and Tri-48T, although some other symbiotic nod and fix genes were detected. On the basis of genotypic and phenotypic analysis, a novel species, Phyllobacterium zundukense sp. nov. (type strain Tri-48T=LMG 30371T=RCAM 03910T), is proposed.
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18 The Family Phyllobacteriaceae
The Prokaryotes, 2014Co-Authors: Anne WillemsAbstract:The family Phyllobacteriaceae belongs to the order Rhizobiales in the Alphaproteobacteria and currently comprises the 72 species in 13 genera: Ahrensia, Aliihoeflea, Aminobacter (including Chelatobacter), Aquamicrobium (including Defluvibacter), Chelativorans, Hoeflea, Lentilitoribacter, Mesorhizobium, Nitratireductor, Phyllobacterium, Pseudahrensia, Pseudaminobacter, and Thermovum. They form a single cluster within the 16S rRNA gene phylogeny. The family consists of environmental (soil, water) and plant-associated bacteria that have a heterotrophic respiratory metabolism with oxygen as terminal electron acceptor. One Aquamicrobium species can use nitrate as an alternative terminal electron acceptor. One Mesorhizobium species is facultatively chemolithotrophic using thiosulfate or elemental sulfur as sole energy source. Candidatus Liberibacter, a group of uncultivated phloem-inhabiting bacteria that are associated with various plant diseases in citrus and Solanaceae or are endophytic in pear plants, is also associated with the family. However, comprehensive phylogenetic analyses indicate the position of this group as a member of the Phyllobacteriaceae is uncertain.
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diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh like gene within the genera microbacterium and starkeya
Microbial Ecology, 2006Co-Authors: Frédéric Zakhia, H. Jeder, Monique Gillis, Bernard Dreyfus, Anne Willems, Philippe De LajudieAbstract:We characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of Tunisia by 16S rDNA polymerase chain reaction (PCR)–restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE), 16S rDNA and 16S–23S rDNA internal transcribed spacer sequencing. Phylogenetically, these isolates belong to the branches containing the genera Inquilinus, Bosea, Rhodopseudomonas, Paracraurococcus, Phyllobacterium, Ochrobactrum, Starkeya, Sphingomonas, Pseudomonas, Agromyces, Microbacterium, Ornithinicoccus, Bacillus, and Paenibacillus. These strains did not induce any nodule formation when inoculated on the wide host spectrum legume species M. atropurpureum (Siratro) and no nodA gene could be amplified by PCR. However, nifH sequences, most similar to those of Sinorhizobium meliloti, were detected within strains related to the genera Microbacterium, Agromyces, Starkeya and Phyllobacterium.
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Phyllobacterium trifolii sp nov nodulating trifolium and lupinus in spanish soils
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, José M. Igual, Felix Fernandezsantosf Fernandezsantos, Nieves Vizcaino, Pedro F Mateos, Eustoquio Martinezmolina, Anne WillemsAbstract:Bacterial strain PETP02T was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02T belongs to a different species. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02T (=LMG 22712T=CECT 7015T). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or β-galactosidase. The DNA G+C content was 56·4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
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Phyllobacterium trifolii sp. nov., nodulating Trifolium and Lupinus in Spanish soils.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, Nieves Vizcaino, Pedro F Mateos, Félix Fernández-santos, Eustoquio Martínez-molina, José Mariano Igual, Anne WillemsAbstract:Bacterial strain PETP02(T) was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02(T) belongs to a different species. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02(T) (=LMG 22712(T)=CECT 7015(T)). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or beta-galactosidase. The DNA G+C content was 56.4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
Encarna Velázquez - One of the best experts on this subject based on the ideXlab platform.
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Rhizobium and Phyllobacterium bacterial inoculants increase bioactive compounds and quality of strawberries cultivated in field conditions.
Food research international (Ottawa Ont.), 2018Co-Authors: José David Flores-félix, Encarna Velázquez, Paula García-fraile, Fernando González-andrés, Luis Silva, Raúl RivasAbstract:Strawberries (Fragaria × ananassa Duch.) are widely demanded by the consumers because they contain several bioactive compounds, mainly vitamin C and anthocyanins, which may be increased by biofertilization with some plant growth promoting bacteria. In this work we have analysed two bacterial strains, PEPV15 and PEPV16, from genera Phyllobacterium and Rhizobium, respectively, which under microcosms conditions were able to promote the strawberry growth, increasing the content of some bioactive compounds, such as vitamin C or organic acids. Here we have analysed the effect on bioactive compounds in strawberries from plants biofertilized with the strains PEPV15 and PEPV16 in field conditions. Under these conditions, the anthocyanin content was increased when plants were biofertilized with the strain PEPV15 and the pelargonidin-3-O-rutinoside content significantly increased. Besides, citric acid, vitamin C and epicatechin contents were significantly higher when either of the two strains was used as biofertilizer. Our results showed that the inoculation with Phyllobacterium and Rhizobium strains is a good agronomical practice, which improve the content of several bioactive compounds of strawberries increasing the beneficial effects on human health.
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Phyllobacterium salinisoli sp. nov., isolated from a Lotus lancerottensis root nodule in saline soil from Lanzarote.
International journal of systematic and evolutionary microbiology, 2018Co-Authors: Milagros León-barrios, Martha-helena Ramírez-bahena, Alvaro Peix, José M. Igual, Encarna VelázquezAbstract:A Gram-negative rod, designated strain LLAN61T, was isolated from a root nodule of Lotus lancerottensis growing in a saline soil sample from Lanzarote (Canary Islands). The strain grew optimally at 0.5 % (w/v) NaCl and tolerated up to 3.5 %. The 16S rRNA gene sequence analysis showed that strain LLAN61T belonged to genus Phyllobacterium and that Phyllobacterium leguminum ORS 1419T and Phyllobacterium myrsinacearum IAM 13584T are the closest related species with 97.93 and 97.86% similarity values, respectively. In the atpD phylogeny, P. leguminum ORS 1419T and P. myrsinacearum ATCC 43591T, sharing similarities of 87.6 and 85.8% respectively, were also the closest species to strain LLAN61T. DNA–DNA hybridization showed an average value of 21 % between strain LLAN61T and P. leguminum LMG 22833T, and 6 % with P. myrsinacearum ATCC 43590T. The predominant fatty acids were C19 : 0 cyclo ω8c and C18 : 1ω6c/C18 : 1ω7c (summed feature 8). The DNA G+C content was 58.0 mol%. Strain LLAN61T differed from its closest relatives in some culture conditions and in assimilation of several carbon sources. Based upon the results of phylogeny, DNA–DNA hybridization, phenotypic tests and fatty acid analysis, this strain should be classified as a novel species of Phyllobacterium for which the name Phyllobacterium salinisoli sp. nov. is proposed (type strain LLAN61T=LMG 30173T = CECT 9417T).
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Current Status of the Taxonomy of Bacteria Able to Establish Nitrogen-Fixing Legume Symbiosis
Microbes for Legume Improvement, 2017Co-Authors: Encarna Velázquez, Martha-helena Ramírez-bahena, Paula García-fraile, Raúl Rivas, Eustoquio Martínez-molinaAbstract:Bacteria forming nitrogen-fixing symbiosis with legumes, classically named rhizobia, currently include more than 100 species distributed in the old genera Allorhizobium, Azorhizobium, Bradyrhizobium, Ensifer (formerly Sinorhizobium), Mesorhizobium and Rhizobium and in the new genera Neorhizobium and Pararhizobium. In addition, several new rhizobia have been described in the twenty-first century belonging, as the classical rhizobia, to the alpha Proteobacteria genera Aminobacter, Devosia, Methylobacterium, Microvirga, Ochrobactrum, Phyllobacterium and Shinella and to the beta Proteobacteria Burkholderia, Paraburkholderia (formerly Burkholderia) and Cupriavidus. These species carry symbiotic genes encoding for nodulation and nitrogen fixation which are located on plasmids or symbiotic islands. These genes determine the host range and confer rhizobia the ability to fix nitrogen in the legume nodules. Depending on the harboured nodulation genes, several symbiovars have recently been described in the classical rhizobia genera. In this chapter, we review the different groups of bacteria able of forming symbiosis with legumes and their classification based on core genes (genera and species) as well as on auxiliary ones (symbiovars).
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plants probiotics as a tool to produce highly functional fruits the case of Phyllobacterium and vitamin c in strawberries
PLOS ONE, 2015Co-Authors: Jose David Floresfelix, Encarna Velázquez, Pedro F Mateos, Eustoquio Martinezmolina, Luis Silva, Lina P Rivera, Marta Marcosgarcia, Paula Garciafraile, Paula B Andrade, Raúl RivasAbstract:The increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alternative to the use of chemical fertilizers, but there are few studies about the effects of plant probiotics on the yield of functional fruits and, especially, on the content of bioactive compounds. In the present work we reported that a strain of genus Phyllobacterium able to produce biofilms and to colonize strawberry roots is able to increase the yield and quality of strawberry plants. In addition, the fruits from plants inoculated with this strain have significantly higher content in vitamin C, one of the most interesting bioactive compounds in strawberries. Therefore the use of selected plant probiotics benefits the environment and human health without agronomical losses, allowing the production of highly functional foods.
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Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Maximo Sánchez, Encarna Velázquez, Maria J Lorite, Alvaro Peix, Juan Sanjuán, Martha-helena Ramírez-bahena, Jorge MonzaAbstract:Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).
Raúl Rivas - One of the best experts on this subject based on the ideXlab platform.
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Rhizobium and Phyllobacterium bacterial inoculants increase bioactive compounds and quality of strawberries cultivated in field conditions.
Food research international (Ottawa Ont.), 2018Co-Authors: José David Flores-félix, Encarna Velázquez, Paula García-fraile, Fernando González-andrés, Luis Silva, Raúl RivasAbstract:Strawberries (Fragaria × ananassa Duch.) are widely demanded by the consumers because they contain several bioactive compounds, mainly vitamin C and anthocyanins, which may be increased by biofertilization with some plant growth promoting bacteria. In this work we have analysed two bacterial strains, PEPV15 and PEPV16, from genera Phyllobacterium and Rhizobium, respectively, which under microcosms conditions were able to promote the strawberry growth, increasing the content of some bioactive compounds, such as vitamin C or organic acids. Here we have analysed the effect on bioactive compounds in strawberries from plants biofertilized with the strains PEPV15 and PEPV16 in field conditions. Under these conditions, the anthocyanin content was increased when plants were biofertilized with the strain PEPV15 and the pelargonidin-3-O-rutinoside content significantly increased. Besides, citric acid, vitamin C and epicatechin contents were significantly higher when either of the two strains was used as biofertilizer. Our results showed that the inoculation with Phyllobacterium and Rhizobium strains is a good agronomical practice, which improve the content of several bioactive compounds of strawberries increasing the beneficial effects on human health.
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Current Status of the Taxonomy of Bacteria Able to Establish Nitrogen-Fixing Legume Symbiosis
Microbes for Legume Improvement, 2017Co-Authors: Encarna Velázquez, Martha-helena Ramírez-bahena, Paula García-fraile, Raúl Rivas, Eustoquio Martínez-molinaAbstract:Bacteria forming nitrogen-fixing symbiosis with legumes, classically named rhizobia, currently include more than 100 species distributed in the old genera Allorhizobium, Azorhizobium, Bradyrhizobium, Ensifer (formerly Sinorhizobium), Mesorhizobium and Rhizobium and in the new genera Neorhizobium and Pararhizobium. In addition, several new rhizobia have been described in the twenty-first century belonging, as the classical rhizobia, to the alpha Proteobacteria genera Aminobacter, Devosia, Methylobacterium, Microvirga, Ochrobactrum, Phyllobacterium and Shinella and to the beta Proteobacteria Burkholderia, Paraburkholderia (formerly Burkholderia) and Cupriavidus. These species carry symbiotic genes encoding for nodulation and nitrogen fixation which are located on plasmids or symbiotic islands. These genes determine the host range and confer rhizobia the ability to fix nitrogen in the legume nodules. Depending on the harboured nodulation genes, several symbiovars have recently been described in the classical rhizobia genera. In this chapter, we review the different groups of bacteria able of forming symbiosis with legumes and their classification based on core genes (genera and species) as well as on auxiliary ones (symbiovars).
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plants probiotics as a tool to produce highly functional fruits the case of Phyllobacterium and vitamin c in strawberries
PLOS ONE, 2015Co-Authors: Jose David Floresfelix, Encarna Velázquez, Pedro F Mateos, Eustoquio Martinezmolina, Luis Silva, Lina P Rivera, Marta Marcosgarcia, Paula Garciafraile, Paula B Andrade, Raúl RivasAbstract:The increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alternative to the use of chemical fertilizers, but there are few studies about the effects of plant probiotics on the yield of functional fruits and, especially, on the content of bioactive compounds. In the present work we reported that a strain of genus Phyllobacterium able to produce biofilms and to colonize strawberry roots is able to increase the yield and quality of strawberry plants. In addition, the fruits from plants inoculated with this strain have significantly higher content in vitamin C, one of the most interesting bioactive compounds in strawberries. Therefore the use of selected plant probiotics benefits the environment and human health without agronomical losses, allowing the production of highly functional foods.
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Phyllobacterium endophyticum sp. nov., isolated from nodules of Phaseolus vulgaris.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: José David Flores-félix, Encarna Velázquez, Lorena Carro, Angel Valverde, Eugenia Cerda-castillo, Paula García-fraile, Raúl RivasAbstract:A strain, PEPV15T, was isolated from a nodule on Phaseolus vulgaris grown in soil in northern Spain. Phylogenetic analyses of 16S rRNA and atpD genes showed that this strain belongs to the genus Phyllobacterium . The most closely related species were, in both cases, Phyllobacterium brassicacearum , Phyllobacterium bourgognense and Phyllobacterium trifolii , the type strains of which gave sequence similarities of 98.9, 98.6 and 98.4 %, respectively, in the 16S rRNA gene and 88.1, 87.5 and 88.7 %, respectively, in the atpD gene. PEPV15T contained Q-10 as the major quinone (88 %) and low amounts of Q-9 (12 %). It differed from its closest relatives in its growth in diverse culture conditions and in the assimilation of several carbon sources. The strain was not able to produce nodules in Phaseolus vulgaris. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium for which the name Phyllobacterium endophyticum sp. nov. is proposed; the type strain is PEPV15T ( = LMG 26470T = CECT 7949T). An emended description of the genus Phyllobacterium is also provided.
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Phyllobacterium trifolii sp nov nodulating trifolium and lupinus in spanish soils
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, José M. Igual, Felix Fernandezsantosf Fernandezsantos, Nieves Vizcaino, Pedro F Mateos, Eustoquio Martinezmolina, Anne WillemsAbstract:Bacterial strain PETP02T was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02T belongs to a different species. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02T (=LMG 22712T=CECT 7015T). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or β-galactosidase. The DNA G+C content was 56·4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
Angel Valverde - One of the best experts on this subject based on the ideXlab platform.
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Phyllobacterium endophyticum sp. nov., isolated from nodules of Phaseolus vulgaris.
International Journal of Systematic and Evolutionary Microbiology, 2012Co-Authors: José David Flores-félix, Encarna Velázquez, Lorena Carro, Angel Valverde, Eugenia Cerda-castillo, Paula García-fraile, Raúl RivasAbstract:A strain, PEPV15T, was isolated from a nodule on Phaseolus vulgaris grown in soil in northern Spain. Phylogenetic analyses of 16S rRNA and atpD genes showed that this strain belongs to the genus Phyllobacterium . The most closely related species were, in both cases, Phyllobacterium brassicacearum , Phyllobacterium bourgognense and Phyllobacterium trifolii , the type strains of which gave sequence similarities of 98.9, 98.6 and 98.4 %, respectively, in the 16S rRNA gene and 88.1, 87.5 and 88.7 %, respectively, in the atpD gene. PEPV15T contained Q-10 as the major quinone (88 %) and low amounts of Q-9 (12 %). It differed from its closest relatives in its growth in diverse culture conditions and in the assimilation of several carbon sources. The strain was not able to produce nodules in Phaseolus vulgaris. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium for which the name Phyllobacterium endophyticum sp. nov. is proposed; the type strain is PEPV15T ( = LMG 26470T = CECT 7949T). An emended description of the genus Phyllobacterium is also provided.
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Phyllobacterium trifolii sp nov nodulating trifolium and lupinus in spanish soils
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, José M. Igual, Felix Fernandezsantosf Fernandezsantos, Nieves Vizcaino, Pedro F Mateos, Eustoquio Martinezmolina, Anne WillemsAbstract:Bacterial strain PETP02T was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02T belongs to a different species. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02T (=LMG 22712T=CECT 7015T). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or β-galactosidase. The DNA G+C content was 56·4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
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Phyllobacterium trifolii sp. nov., nodulating Trifolium and Lupinus in Spanish soils.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, Nieves Vizcaino, Pedro F Mateos, Félix Fernández-santos, Eustoquio Martínez-molina, José Mariano Igual, Anne WillemsAbstract:Bacterial strain PETP02(T) was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02(T) belongs to a different species. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02(T) (=LMG 22712(T)=CECT 7015(T)). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or beta-galactosidase. The DNA G+C content was 56.4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
Pedro F Mateos - One of the best experts on this subject based on the ideXlab platform.
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plants probiotics as a tool to produce highly functional fruits the case of Phyllobacterium and vitamin c in strawberries
PLOS ONE, 2015Co-Authors: Jose David Floresfelix, Encarna Velázquez, Pedro F Mateos, Eustoquio Martinezmolina, Luis Silva, Lina P Rivera, Marta Marcosgarcia, Paula Garciafraile, Paula B Andrade, Raúl RivasAbstract:The increasing interest in the preservation of the environment and the health of consumers is changing production methods and food consumption habits. Functional foods are increasingly demanded by consumers because they contain bioactive compounds involved in health protection. In this sense biofertilization using plant probiotics is a reliable alternative to the use of chemical fertilizers, but there are few studies about the effects of plant probiotics on the yield of functional fruits and, especially, on the content of bioactive compounds. In the present work we reported that a strain of genus Phyllobacterium able to produce biofilms and to colonize strawberry roots is able to increase the yield and quality of strawberry plants. In addition, the fruits from plants inoculated with this strain have significantly higher content in vitamin C, one of the most interesting bioactive compounds in strawberries. Therefore the use of selected plant probiotics benefits the environment and human health without agronomical losses, allowing the production of highly functional foods.
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Phyllobacterium trifolii sp nov nodulating trifolium and lupinus in spanish soils
International Journal of Systematic and Evolutionary Microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, José M. Igual, Felix Fernandezsantosf Fernandezsantos, Nieves Vizcaino, Pedro F Mateos, Eustoquio Martinezmolina, Anne WillemsAbstract:Bacterial strain PETP02T was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02T belongs to a different species. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02T (=LMG 22712T=CECT 7015T). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or β-galactosidase. The DNA G+C content was 56·4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.
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Phyllobacterium trifolii sp. nov., nodulating Trifolium and Lupinus in Spanish soils.
International journal of systematic and evolutionary microbiology, 2005Co-Authors: Angel Valverde, Encarna Velázquez, Raúl Rivas, Nieves Vizcaino, Pedro F Mateos, Félix Fernández-santos, Eustoquio Martínez-molina, José Mariano Igual, Anne WillemsAbstract:Bacterial strain PETP02(T) was isolated from nodules of Trifolium pratense growing in a Spanish soil. Phylogenetic analysis of the 16S rRNA gene sequence showed that this strain represents a member of the genus Phyllobacterium. However, divergence found with the 16S rRNA gene sequence of the single recognized species of this genus, Phyllobacterium myrsinacearum, indicated that strain PETP02(T) belongs to a different species. The results of DNA-DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain represents a novel species of the genus Phyllobacterium, for which the name Phyllobacterium trifolii sp. nov. is proposed. The type strain is PETP02(T) (=LMG 22712(T)=CECT 7015(T)). This strain was strictly aerobic and used several carbohydrates as carbon source. It was not able to reduce nitrate. Aesculin hydrolysis was negative. It did not produce urease, arginine dihydrolase, gelatinase or beta-galactosidase. The DNA G+C content was 56.4 mol%. The nodD gene of this strain showed a sequence closely related to those of strains able to nodulate Lupinus. Infectivity tests showed that this strain is able to produce nodules in both Trifolium repens and Lupinus albus.