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Youngmin Lee - One of the best experts on this subject based on the ideXlab platform.
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agrimonia Pilosa ledeb aqueous extract improves impaired glucose tolerance in high fat diet fed rats by decreasing the inflammatory response
BMC Complementary and Alternative Medicine, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. Pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. Pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05). Our findings indicate that A. Pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
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Agrimonia Pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
BMC, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Abstract Background Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Methods Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. Results The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p
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Effects of Agrimonia Pilosa Ledeb. Water Extract on α-Glucosidase Inhibition and Glucose Uptake in C2C12 Skeletal Muscle Cells
2016Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/ ㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A
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effects of agrimonia Pilosa ledeb water extract on α glucosidase inhibition and glucose uptake in c2c12 skeletal muscle cells
The Korean Journal of Food And Nutrition, 2013Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Abstract Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A. Pilosa is useful in the prevention of diabetes and the treatment of hyperglycemic disorders. Key words: anti-diabetes, insulin resistance, skeletal muscle cells, 2-NBDG, Glut 4
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estrogen like activity of aqueous extract from agrimonia Pilosa ledeb in mcf 7 cells
BMC Complementary and Alternative Medicine, 2012Co-Authors: Youngmin Lee, Ji Hyun Bae, Hwanhee Jang, Jung Bong Kim, Jong Suk Lee, Pansoo Kim, Haeng Ran KimAbstract:Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia Pilosa Ledeb. The estrogenic activity of A. Pilosa was investigated by using several in vitro assays. The binding activity of A. Pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. Pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. Pilosa on the expression of 3 estrogen-dependent genes was assessed. Using liquid chromatography-mass spectrometry, the 3 major peaks of A. Pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. Pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. Pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05). These results suggest A. Pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.
Hwanhee Jang - One of the best experts on this subject based on the ideXlab platform.
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agrimonia Pilosa ledeb aqueous extract improves impaired glucose tolerance in high fat diet fed rats by decreasing the inflammatory response
BMC Complementary and Alternative Medicine, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. Pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. Pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05). Our findings indicate that A. Pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
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Agrimonia Pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
BMC, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Abstract Background Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Methods Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. Results The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p
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Effects of Agrimonia Pilosa Ledeb. Water Extract on α-Glucosidase Inhibition and Glucose Uptake in C2C12 Skeletal Muscle Cells
2016Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/ ㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A
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effects of agrimonia Pilosa ledeb water extract on α glucosidase inhibition and glucose uptake in c2c12 skeletal muscle cells
The Korean Journal of Food And Nutrition, 2013Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Abstract Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A. Pilosa is useful in the prevention of diabetes and the treatment of hyperglycemic disorders. Key words: anti-diabetes, insulin resistance, skeletal muscle cells, 2-NBDG, Glut 4
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estrogen like activity of aqueous extract from agrimonia Pilosa ledeb in mcf 7 cells
BMC Complementary and Alternative Medicine, 2012Co-Authors: Youngmin Lee, Ji Hyun Bae, Hwanhee Jang, Jung Bong Kim, Jong Suk Lee, Pansoo Kim, Haeng Ran KimAbstract:Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia Pilosa Ledeb. The estrogenic activity of A. Pilosa was investigated by using several in vitro assays. The binding activity of A. Pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. Pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. Pilosa on the expression of 3 estrogen-dependent genes was assessed. Using liquid chromatography-mass spectrometry, the 3 major peaks of A. Pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. Pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. Pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05). These results suggest A. Pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.
Haeng Ran Kim - One of the best experts on this subject based on the ideXlab platform.
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agrimonia Pilosa ledeb aqueous extract improves impaired glucose tolerance in high fat diet fed rats by decreasing the inflammatory response
BMC Complementary and Alternative Medicine, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. Pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. Pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05). Our findings indicate that A. Pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
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Agrimonia Pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
BMC, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Abstract Background Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Methods Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. Results The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p
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estrogen like activity of aqueous extract from agrimonia Pilosa ledeb in mcf 7 cells
BMC Complementary and Alternative Medicine, 2012Co-Authors: Youngmin Lee, Ji Hyun Bae, Hwanhee Jang, Jung Bong Kim, Jong Suk Lee, Pansoo Kim, Haeng Ran KimAbstract:Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia Pilosa Ledeb. The estrogenic activity of A. Pilosa was investigated by using several in vitro assays. The binding activity of A. Pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. Pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. Pilosa on the expression of 3 estrogen-dependent genes was assessed. Using liquid chromatography-mass spectrometry, the 3 major peaks of A. Pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. Pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. Pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05). These results suggest A. Pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.
Jung Bong Kim - One of the best experts on this subject based on the ideXlab platform.
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agrimonia Pilosa ledeb aqueous extract improves impaired glucose tolerance in high fat diet fed rats by decreasing the inflammatory response
BMC Complementary and Alternative Medicine, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. Pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. Pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05). Our findings indicate that A. Pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
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Agrimonia Pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
BMC, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Abstract Background Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Methods Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. Results The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p
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estrogen like activity of aqueous extract from agrimonia Pilosa ledeb in mcf 7 cells
BMC Complementary and Alternative Medicine, 2012Co-Authors: Youngmin Lee, Ji Hyun Bae, Hwanhee Jang, Jung Bong Kim, Jong Suk Lee, Pansoo Kim, Haeng Ran KimAbstract:Postmenopausal women experience estrogen deficiency-related menopausal symptoms (e.g., hot flashes and mood swings) and a dramatic increase in the incidence of chronic diseases. Although estrogen-replacement therapy (ERT) can reduce mortality from cardiovascular disease and improve osteoporosis and menopausal symptoms, its side effects have limited recent use. This study investigated the estrogen-like activity of aqueous extract from Agrimonia Pilosa Ledeb. The estrogenic activity of A. Pilosa was investigated by using several in vitro assays. The binding activity of A. Pilosa on estrogen receptors was examined using a fluorescence polarization-based competitive binding assay. The proliferative activity of A. Pilosa was also examined using MCF-7 cells. Furthermore, the effect of A. Pilosa on the expression of 3 estrogen-dependent genes was assessed. Using liquid chromatography-mass spectrometry, the 3 major peaks of A. Pilosa aqueous extract were identified as apigenin-hexose, luteolin-glucuronide, and apigenin-glucuronide. The aqueous extract induced the proliferation of estrogen receptor-positive MCF-7 cells (p < 0.05). A. Pilosa-stimulated proliferation was blocked on adding the estrogen antagonist ICI 182,780. Moreover, A. Pilosa treatment increased the mRNA expression of the estrogen-responsive genes pS2 and PR (p < 0.05). These results suggest A. Pilosa can be used to improve estrogen deficiency-related menopausal symptoms or to treat diseases in postmenopausal women.
Miju Kim - One of the best experts on this subject based on the ideXlab platform.
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agrimonia Pilosa ledeb aqueous extract improves impaired glucose tolerance in high fat diet fed rats by decreasing the inflammatory response
BMC Complementary and Alternative Medicine, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p < 0.05); however, supplementation with A. Pilosa decreased liver weight. Blood glucose levels in the HFA group were lower than levels measured in the HF group 30, 60, and 120 min after glucose administration (p < 0.05). In addition, dietary A. Pilosa supplementation decreased tumor necrosis factor α and interleukin 6 levels, while increasing serum adiponectin concentrations (p < 0.05 vs. the HF group). These effects were accompanied by reduced hepatic and adipose tissue expression of inflammation-related genes such as Tnf and Il1b (p < 0.05). Our findings indicate that A. Pilosa aqueous extract can ameliorate insulin resistance in high-fat diet-fed rats by decreasing the inflammatory response.
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Agrimonia Pilosa Ledeb. aqueous extract improves impaired glucose tolerance in high-fat diet-fed rats by decreasing the inflammatory response
BMC, 2017Co-Authors: Hwanhee Jang, Miju Kim, Songyee Nam, Jung Bong Kim, Haeng Ran Kim, Jeong Sook Choi, Youngmin LeeAbstract:Abstract Background Agrimonia Pilosa Ledeb. is a medicinal plant with physiological activities such as anti-cancer, antioxidant, anti-inflammatory activities and in vitro anti-diabetic activity. However, the effects of aqueous extracts from A. Pilosa on insulin-resistant rats have not yet been examined. We investigated the effects of aqueous extract from A. Pilosa on impaired glucose metabolism induced by a high-fat diet in rats. Methods Male Sprague-Dawley rats were assigned to the following groups: normal-fat diet (NF, n = 9); high-fat diet (HF, n = 9); high-fat diet with 0.1% A. Pilosa aqueous extract (HFA, n = 10). Experimental diets were administered for 16 weeks. At the end of the treatment, liver and fat tissues were isolated, and serum was collected for biochemical analysis. Results The HF group rats had a significantly higher liver weight than the NF group rats did, and increased hepatic lipid accumulation (p
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Effects of Agrimonia Pilosa Ledeb. Water Extract on α-Glucosidase Inhibition and Glucose Uptake in C2C12 Skeletal Muscle Cells
2016Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/ ㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A
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effects of agrimonia Pilosa ledeb water extract on α glucosidase inhibition and glucose uptake in c2c12 skeletal muscle cells
The Korean Journal of Food And Nutrition, 2013Co-Authors: Sangmi Kim, Youngmin Lee, Miju Kim, Songyee Nam, Sunghee Kim, Hwanhee JangAbstract:Abstract Agrimonia Pilosa Ledeb. is a medicinal plant with anti-tumor, anti-oxidant, anti-inflammatory and anti-hyperglycemic activities. However, few studies of the anti-diabetic effect of A. Pilosa on insulin resistance status have been performed. In the present study, the anti-diabetic effect of A. Pilosa water extract (AP) was determined by investigating its α-glucosidase inhibitory property, glucose utilization, and uptake, as well as insulin resistance mechanism of action in C2C12 skeletal muscle cells. Compared to positive control (acarbose), AP (10 ㎎/㎖) showed a similar α-glucosidase inhibitory capacity. Glucose uptake was significantly increased by 1 μM insulin treatment (p<0.05). However, palmitic acid (FFA, 1 mM) induced muscle insulin resistance and glucose uptake dysfunction. On the other hand, AP (10 ㎍/㎖) was capable of reversing the FFA-induced insulin resistance in C2C12 myotubes. Compared to control, AP (100 ㎍/㎖ without insulin) significantly increased the utilization of glucose (p<0.05) in C2Cl2 myotubes cultured in normal glucose (7 mM). AP treatment significantly increased the relative mRNA and protein expression levels of Akt. In particular, the effect of A. Pilosa on the insulin signaling system is associated with the up-regulation of Akt genes and glucose uptake in C2Cl2 myotubes. These results suggest that A. Pilosa is useful in the prevention of diabetes and the treatment of hyperglycemic disorders. Key words: anti-diabetes, insulin resistance, skeletal muscle cells, 2-NBDG, Glut 4
