Pinguecula

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Vitor F O Miranda - One of the best experts on this subject based on the ideXlab platform.

  • flower nectar trichome structure of carnivorous plants from the genus butterworts pinguicula l lentibulariaceae
    Protoplasma, 2020
    Co-Authors: Krzysztof Lustofin, Piotr świątek, Vitor F O Miranda, Bartosz J Plachno
    Abstract:

    Pinguicula (Lentibulariaceae) is a genus comprising around 96 species of herbaceous, carnivorous plants, which are extremely diverse in flower size, colour and spur length and structure as well as pollination strategy. In Pinguicula, nectar is formed in the flower spur; however, there is a gap in the knowledge about the nectary trichome structure in this genus. Our aim was to compare the nectary trichome structure of various Pinguicula species in order to determine whether there are any differences among the species in this genus. The taxa that were sampled were Pinguicula moctezumae, P. moranensis, P. rectifolia, P. emarginata and P. esseriana. We used light microscopy, histochemistry, scanning and transmission electron microscopy to address those aims. We show a conservative nectary trichome structure and spur anatomy in various Mexican Pinguicula species. The gross structural similarities between the examined species were the spur anatomy, the occurrence of papillae, the architecture of the nectary trichomes and the ultrastructure characters of the trichome cells. However, there were some differences in the spur length, the size of spur trichomes, the occurrence of starch grains in the spur parenchyma and the occurrence of cell wall ingrowths in the terminal cells of the nectary trichomes. Similar nectary capitate trichomes, as are described here, were recorded in the spurs of species from other Lentibulariaceae genera. There are many ultrastructural similarities between the cells of nectary trichomes in Pinguicula and Utricularia.

  • the chloroplast genome of utricularia reniformis sheds light on the evolution of the ndh gene complex of terrestrial carnivorous plants from the lentibulariaceae family
    PLOS ONE, 2016
    Co-Authors: Saura R Silva, Vitor F O Miranda, Todd P Michael, Daniel G Pinheiro, Yani C A Diaz, Helen A Penha, Camila C Fernandes, Alessandro M Varani
    Abstract:

    Lentibulariaceae is the richest family of carnivorous plants spanning three genera including Pinguicula, Genlisea, and Utricularia. Utricularia is globally distributed, and, unlike Pinguicula and Genlisea, has both aquatic and terrestrial forms. In this study we present the analysis of the chloroplast (cp) genome of the terrestrial Utricularia reniformis. U. reniformis has a standard cp genome of 139,725bp, encoding a gene repertoire similar to essentially all photosynthetic organisms. However, an exclusive combination of losses and pseudogenization of the plastid NAD(P)H-dehydrogenase (ndh) gene complex were observed. Comparisons among aquatic and terrestrial forms of Pinguicula, Genlisea, and Utricularia indicate that, whereas the aquatic forms retained functional copies of the eleven ndh genes, these have been lost or truncated in terrestrial forms, suggesting that the ndh function may be dispensable in terrestrial Lentibulariaceae. Phylogenetic scenarios of the ndh gene loss and recovery among Pinguicula, Genlisea, and Utricularia to the ancestral Lentibulariaceae cladeare proposed. Interestingly, RNAseq analysis evidenced that U. reniformis cp genes are transcribed, including the truncated ndh genes, suggesting that these are not completely inactivated. In addition, potential novel RNA-editing sites were identified in at least six U. reniformis cp genes, while none were identified in the truncated ndh genes. Moreover, phylogenomic analyses support that Lentibulariaceae is monophyletic, belonging to the higher core Lamiales clade, corroborating the hypothesis that the first Utricularia lineage emerged in terrestrial habitats and then evolved to epiphytic and aquatic forms. Furthermore, several truncated cp genes were found interspersed with U. reniformis mitochondrial and nuclear genome scaffolds, indicating that as observed in other smaller plant genomes, such as Arabidopsis thaliana, and the related and carnivorous Genlisea nigrocaulis and G. hispidula, the endosymbiotic gene transfer may also shape the U. reniformis genome in a similar fashion. Overall the comparative analysis of the U. reniformis cp genome provides new insight into the ndh genes and cp genome evolution of carnivorous plants from Lentibulariaceae family.

  • dominguez y panfet valdes c m miranda v f o 2014 pinguicula filifolia subsp alba lentibulariaceae a new subspecies with an extremely restricted distribution in pinar del rio cuba phytotaxa 158 1 85 92
    Phytotaxa, 2015
    Co-Authors: Yoannis Dominguez, Cristina Panfet Valdes, Vitor F O Miranda
    Abstract:

    The carnivorous plant  Pinguicula filifolia  occurs in western Cuba and shows a restricted distribution due to habitat specificity associated with poor wet soils. Its populations are distributed mainly in south Pinar del Rio (Cuba) and in Isla de la Juventud. Plants from one isolated location in NW Pinar del Rio shows morphological differences with respect to the rest of the known populations observed in nature. Subsequent detailed morphological analysis of specimens led to the description of  Pinguicula filifolia  subsp.  alba  as a new subspecies. It differs from the typical subspecies in several reproductive traits involving flower, fruit and seed characters and is so far known only from the type locality.

  • inter and intra specific diversity of cuban pinguicula lentibulariaceae based on morphometric analyses and its relation with geographical distribution
    Plant Ecology & Diversity, 2014
    Co-Authors: Yoannis Dominguez, Saura R Silva, Cristina Panfet Valdes, Vitor F O Miranda
    Abstract:

    Background: Phenotypic variations have been observed in populations of west Cuban Pinguicula species. Such populations occur in patches under different ecological conditions associated with specialised habitats and separated by geographical and ecological barriers, which can lead to morphological differentiation.Aims: To analyse morphological diversity among species and populations of Cuban Pinguicula; and to test if morphological variability is associated with geographical distribution and distance between populations.Methods: We sampled a total of eight populations of P. albida, P. cubensis and P. filifolia and tested for morphometric differences among them by Principal Component Analysis (PCA) and analysis of variance (ANOVA) based on 31 quantitative traits. We also assessed the geographical isolation with respect to morphological distance.Results: The studied species showed high morphological variability at both species and population level. Reproductive characteristics appeared to be more promissory ...

  • pinguicula filifolia subsp alba lentibulariaceae a new subspecies with an extremely restricted distribution in pinar del rio cuba
    Phytotaxa, 2014
    Co-Authors: Yoannis Dominguez, Cristina Panfet Valdes, Vitor F O Miranda
    Abstract:

    The carnivorous plant Pinguicula filifolia occurs in western Cuba and shows a restricted distribution due to habitat specificity associated with poor wet soils. Its populations are distributed mainly in south Pinar del Rio (Cuba) and in Isla de la Juventud. Plants from one isolated location in NW Pinar del Rio shows morphological differences with respect to the rest of the known populations observed in nature. Subsequent detailed morphological analysis of specimens led to the description of Pinguicula filifolia subsp. alba as a new subspecies. It differs from the typical subspecies in several reproductive traits involving flower, fruit and seed characters and is so far known only from the type locality.

Paul Mitchell - One of the best experts on this subject based on the ideXlab platform.

  • pterygium Pinguecula and 5 year incidence of cataract
    American Journal of Ophthalmology, 2005
    Co-Authors: Thuan Quoc Pham, Jie Jin Wang, Elena Rochtchina, Paul Mitchell
    Abstract:

    Purpose To assess longitudinal associations between pterygium, Pinguecula, and 5-year incident cataract. Design Population-based cohort study. Methods The Blue Mountains Eye Study examined 3,654 residents aged ≥49 years during 1992 to 1994 and reexamined 2,335 (75.1% of survivors) 5 years later. Slit-lamp examination recorded pterygium and Pinguecula. Cataract was assessed using masked grading of lens photographs. Incidence was assessed in participants without cataract at baseline. Eye-specific data were analyzed using generalized estimating equation models, adjusting for age, sex, smoking, diabetes, and corticosteroid use. Results Pinguecula was associated with a borderline-significant, increased risk of developing cortical cataract (adjusted odds ratio, 1.3; 95% confidence interval, 1.0 to 1.7). We found no significant association between baseline pterygium and the incidence of cortical, nuclear, or posterior subcapsular cataract. Conclusions Longitudinal data from the same study population provide weak support for cross-sectional associations between Pinguecula and cortical cataract previously reported from our study.

  • pterygium Pinguecula and the five year incidence of age related maculopathy
    American Journal of Ophthalmology, 2005
    Co-Authors: Thuan Quoc Pham, Jie Jin Wang, Elena Rochtchina, Paul Mitchell
    Abstract:

    Purpose To assess the relationship between baseline pterygium and Pinguecula and the five-year incidence of age-related maculopathy (ARM). Design Population-based longitudinal study. Methods The Blue Mountains Eye Study examined 3654 residents aged 49+ years during 1992 to 1994 and then re-examined 2335 (75.1% of survivors) after five years. Retinal photographs were graded using the Wisconsin Age-Related Maculopathy Grading System. Slit-lamp examination recorded pterygium and Pinguecula. Eye-specific data were analyzed using generalized estimating equation models. Results After adjusting for age, gender, and smoking, eyes with pterygium or previous pterygium surgery had a higher risk of incident late ARM, odds ratio (OR) 3.3, 95% confidence interval (CI) 1.1 to 10.3, early ARM (OR 1.8, CI 1.1 to 2.9) and soft drusen (OR 2.0, CI 1.9 to 3.4), than eyes without pterygium. We found no association between Pinguecula and incident ARM. Conclusions This study found that pterygium was associated with a two- to threefold increased risk of incident late and early ARM.

  • cataract associations with Pinguecula and pterygium the blue mountains eye study
    American Journal of Ophthalmology, 1998
    Co-Authors: Ridia Lim, Paul Mitchell, Robert G Cumming
    Abstract:

    Abstract PURPOSE: To determine associations between cataract types and Pinguecula and pterygium. METHODS: The Blue Mountains Eye Study examined 3,654 persons aged 49 to 97 years near Sydney, Australia. A questionnaire was used to collect information on cataract risk factors. Slit-lamp examination recorded Pinguecula and pterygium. Masked lens photograph grading assessed cataract. RESULTS: Pinguecula, found in 2,418 right eyes and 2,437 left eyes, was statistically significantly associated with cortical cataract (odds ratio [OR] = 1.40) after multivariate adjustment. Pterygium, found in 199 right eyes and 188 left eyes, was associated with posterior subcapsular cataract (OR = 1.90). CONCLUSIONS: Associations were found between cataract and the presence of either Pinguecula or pterygium. These findings provide indirect support for Watermen Study findings, which link ultraviolet radiation to cataract.

  • prevalence of pterygium and Pinguecula the blue mountains eye study
    Australian and New Zealand Journal of Ophthalmology, 1998
    Co-Authors: Jai Panchapakesan, Fleur Hourihan, Paul Mitchell
    Abstract:

    Purpose: The present study aimed to describe the prevalence of pterygium and Pinguecula in an older population and to examine associations with skin, hair and eye colour, skin sun sensitivity, sun-related skin damage and skin cancer. Methods: The Blue Mountains Eye Study examined 3564 people aged 49 years or older Slit-lamp examination recorded pterygium and Pinguecula and a questionnaire was used to collect information on physical variables. Results: Two hundred and sixty-six subjects (7.3%) had pterygium (or had a history of pterygium surgery) and 2521 (69.5%) had Pinguecula present in either eye. Significantly more men (11%) than women (4.5%) had pterygium (odds ratio (OR) 2.63; 95% confidence interval (Cl) 2.03-3.42). This sex difference was also found for Pinguecula, present in 73.6 and 66.3% of men and women, respectively (OR 1.5; 95% Cl 1.3-1.7). A slight age-related increase in prevalence was found for both pterygium and Pinguecula. Conclusions: The study found significant associations between pterygium and increased pigmentation (skin and hair colour), decreased skin sun sensitivity and sun-related skin damage. The age and sex-specific pterygium prevalence rates in the present study are similar to rates found in non-Aboriginals examined in the 1980 Australian Trachoma Programme.

Shiro Amano - One of the best experts on this subject based on the ideXlab platform.

  • conjunctivochalasis associations with Pinguecula in a hospital based study
    Acta Ophthalmologica, 2012
    Co-Authors: Tatsuya Mimura, Hideharu Funatsu, Hidetaka Noma, Mikiro Mori, Hiroto Obata, Tomohiko Usui, Satoru Yamagami, Shiro Amano
    Abstract:

    . Purpose:  To assess the relationship between the prevalence and severity of conjunctivochalasis and Pinguecula. Methods:  Cross-sectional, consecutive case study conducted at the university hospital of University of Tokyo Graduate School of Medicine. A total of 1061 patients aged from 1 to 94 years were enrolled. The grade and other parameters of conjunctivochalasis (classified into three locations: nasal, middle and temporal) and the grade of Pinguecula located on the nasal or temporal conjunctiva were determined in all subjects. Patients were also divided into 5 or 10 age groups. Results:  The severity of conjunctivochalasis affecting the nasal and temporal bulbar conjunctiva was significantly correlated with the grade of Pinguecula located on the nasal and temporal conjunctiva in each age group (p < 0.05). Pinguecula was independently associated with conjunctivochalasis (nasal: odds ratio [OR] = 1.44; temporal: OR = 1.43) after adjustment for age. Conclusion:  This was the first assessment of the relation between the grade of conjunctivochalasis and Pinguecula in a large consecutive series of patients. Our results suggest that the prevalence and severity of conjunctivochalasis are related to the presence of Pinguecula.

  • Pinguecula and diabetes mellitus
    Cornea, 2012
    Co-Authors: Tatsuya Mimura, Hideharu Funatsu, Hidetaka Noma, Mikiro Mori, Hiroto Obata, Tomohiko Usui, Satoru Yamagami, Shiro Amano
    Abstract:

    Purpose:To assess the relationship between age and the incidence and severity of Pinguecula in patients with diabetes mellitus, and to compare the grade of Pinguecula between control subjects and patients with diabetes.Methods:A total of 160 patients with type 2 diabetes aged 31 to 70 years (DM grou

  • severity and determinants of Pinguecula in a hospital based population
    Eye & Contact Lens-science and Clinical Practice, 2011
    Co-Authors: Tatsuya Mimura, Hideharu Funatsu, Hidetaka Noma, Mikiro Mori, Hiroto Obata, Tomohiko Usui, Satoru Yamagami, Kazuhiro Dou, Shiro Amano
    Abstract:

    Objectives:Pinguecula is a relatively frequent disease; however, there have been no reports about the grade of Pinguecula. We investigated the relationship between age and the prevalence and severity of Pinguecula by using a grading system and compared the grade of Pinguecula between men and women a

  • contact lens induced subconjunctival hemorrhage
    American Journal of Ophthalmology, 2010
    Co-Authors: Tatsuya Mimura, Hideharu Funatsu, Hidetaka Noma, Mikiro Mori, Tomohiko Usui, Satoru Yamagami, Shiro Amano
    Abstract:

    Purpose To present the first detailed assessment of the clinical features of CL-induced subconjunctival hemorrhage and associated risk factors. Design Cross-sectional and case-control study of age-matched randomized groups. Methods A total of 45 CL wearers with subconjunctival hemorrhage aged 18 to 45 years (CL-Hemorrhage group), 200 age-matched healthy control subjects (non-CL group), and 200 age-matched CL wearers (CL group) were enrolled. The conjunctiva was divided into the following 8 equal areas: superior, superior/nasal, nasal, inferior/nasal, inferior, inferior/temporal, temporal, and superior/temporal. The site of hemorrhage, the grade, and other parameters of conjunctivochalasis at 3 locations (nasal, middle, and temporal), and the grade of Pinguecula on the nasal or temporal conjunctiva were determined in all subjects. Results Typically, subconjunctival hemorrhage affected 1 or 2 regions of the temporal conjunctiva. The grade of conjunctivochalasis and Pinguecula was higher in both the affected and unaffected eyes of the CL-Hemorrhage group than the non-CL and CL groups (all P P P Conclusions These results suggest that the major risk factors for CL-induced subconjunctival hemorrhage are conjunctivochalasis and Pinguecula.

  • Pinguecula and contact lenses
    Eye, 2010
    Co-Authors: Tatsuya Mimura, Hideharu Funatsu, Hidetaka Noma, Mikiro Mori, Hiroto Obata, Tomohiko Usui, Satoru Yamagami, Hiroki Yamamoto, Norihiko Honda, Shiro Amano
    Abstract:

    To assess the relationship between age and the incidence and severity (determined by a grading system) of Pinguecula in contact lens (CL) wearers, and to compare the grade of Pinguecula between CL wearers and non-wearers. A total of 600 CL wearers (94 wore hard CLs (HCLs) and 506 wore soft CLs (SCLs)) aged 11–60 years and 579 non-wearers aged 10–60 years were enrolled. The age, gender, medical history, ocular history, and grade of Pinguecula at two locations (nasal and temporal) were determined in all subjects. There was an age-related increase in the grade of Pinguecula among both CL wearers and non-wearers. The grade of Pinguecula at the temporal conjunctiva was higher in CL wearers than in non-wearers (P=0.01907), whereas it was higher in HCL wearers than SCL wearers at both the nasal and temporal conjunctiva (P<0.00001 and P<0.00001). This was the first assessment of the severity of Pinguecula in a large consecutive series of CL wearers. Our results suggest that the use of CLs is an important risk factor for Pinguecula.

Lorenzo Peruzzi - One of the best experts on this subject based on the ideXlab platform.

  • disentangling phylogenetic relationships in a hotspot of diversity the butterworts pinguicula l lentibulariaceae endemic to italy
    PLOS ONE, 2016
    Co-Authors: Olga De Castro, Gianluigi Bacchetta, Michele Innangi, Antonietta Di Maio, Bruno Menale, Mathias M Pires, Virgile Noble, G Gestri, Fabio Conti, Lorenzo Peruzzi
    Abstract:

    The genus Pinguicula (Lentibulariaceae) consists of about 100 carnivorous species, also known as butterworts. Eleven taxa are endemic to Italy, which represents a biodiversity hotspot for butterworts in Europe. The aim of our study was to provide a phylogenetic framework for the Italian endemics, in order to: a) investigate the relationships between species in this group; b) evaluate their actual taxonomic value. To achieve this, we analysed all the taxa endemic to Italy, along with several other species, by means of ITS nrDNA analysis. Our results clarify the relationships between Italian endemics and other Pinguicula taxa identifying a basal polytomy defined by five clades. All of the Italian endemics (with the exception of P. lavalvae) fall within a single large clade, which includes P. vulgaris and allied species. Among them, P. poldinii represents the most isolated lineage. Other taxa show strong molecular similarities and form a single subclade, although their taxonomic ranks can be retained. Pinguicula lattanziae sp. nov., seemingly endemic to Liguria (NW Italy), is also described.

  • a new diploid butterwort species pinguicula lentibulariaceae from sardinia
    Phytotaxa, 2014
    Co-Authors: Gianluigi Bacchetta, Marcello Cannas, Lorenzo Peruzzi
    Abstract:

    A new diploid species belonging to Pinguicula sect. Pinguicula is described from Sardinia, an island where no butterwort was previously reported from. The taxonomic relationship of P. sehuensis sp. nov. with other species is briefly discussed.

  • a new butterwort species pinguicula lentibulariaceae from northern apennine italy
    Plant Biosystems, 2013
    Co-Authors: Lorenzo Peruzzi, Gaia Gestri
    Abstract:

    A morphometric and taxonomic study of Pinguicula in Northern Apennine was carried out. A new species belonging to sect. Pinguicula, previously referred erroneously to P. vulgaris L. and/or P. leptoceras Rchb., is here described as P. christinae sp. nov. The taxonomic relationship of P. christinae with other octoploid species, such as P. apuana Casper and Ansaldi, P. fiorii Tammaro and Pace, and P. vulgaris s.l. (including also its central Italian endemic subspecies) is discussed.

Jiwon Kwon - One of the best experts on this subject based on the ideXlab platform.

  • the improvement of dry eye symptoms after Pinguecula excision and conjunctival autograft with fibrin glue
    Journal of Ophthalmology, 2019
    Co-Authors: Jinho Jeong, Gabriel M Rand, Taejung Kwon, Jiwon Kwon
    Abstract:

    Purpose. To evaluate the association between Pinguecula excision and subsequent improvement in dry eye syndrome. Methods. We included 30 consecutive patients with primary nasal Pinguecula and dry eye symptoms undergoing ocular surgery for the first time. Criteria for Pinguecula excision surgery were nasal location, yellowish color, and protrusion of conjunctiva at least 2 times thicker than adjacent normal conjunctiva as measured by anterior segment optical coherence tomography. Our primary outcomes were 3-month postoperative changes in tear film breakup time (TBUT), Schirmer test, and a dry eye symptom score. Results. 30 eyes from 30 different patients (12 men and 18 women) underwent Pinguecula excision and conjunctival autografting using fibrin glue. The mean age was 42.5 ± 8.35 (range 28–63) years. The preoperative protrusion ratio of Pinguecula was 2.33 ± 0.28 (range 2.00–2.90). Mean preoperative TBUT, Schirmer test, and dry eye symptom scores were 5.10 ± 1.27 seconds, 6.07 ± 2.27 mm, and 2.80 ± 0.76 points. Mean postoperative 3-month TBUT, Schirmer test, and dry eye symptom scores were 7.80 ± 1.13 seconds, 7.27 ± 2.02 mm, and 0.30 ± 0.47 points, respectively. The median pre- and postoperative changes were found to be statistically significant by Wilcoxon signed-rank tests for TBUT, Schirmer test score, and dry eye symptom score. Conclusion. Surgical excision of Pinguecula and conjunctival autograft using fibrin glue is an effective and safe method to improve symptoms of dry eye syndrome.

  • vascular regression after Pinguecula excision and conjunctival autograft using fibrin glue
    Eye & Contact Lens-science and Clinical Practice, 2017
    Co-Authors: Seungil Jung, Jiwon Kwon, Ho Sik Hwang, Roy S Chuck
    Abstract:

    To determine the change in local vascularization after Pinguecula excision and conjunctival autograft secured with fibrin glue at 12 months. Thirty-one eyes of 31 patients which underwent excision and conjunctival autografting with fibrin glue were retrospectively reviewed. Anterior segment photography was obtained before and after the surgery. Cosmetic outcome was evaluated by patient self-grading (five-point scale; excellent (5), good (4), acceptable (3), poor (2), and very poor (1)), and medical evaluation of treatment outcome was based on subjective evaluation of vascularization on anterior segment photography (four-point severity scale; 0 [low] to 3 [high]). Clinical outcome and complications are reported at 12 months. Overall cosmetic results were excellent or good in 93.5% (29 of 31) (score 4.68±0.60). Anterior segment photography showed that most cases demonstrated complete removal of Pinguecula and regression of surrounding vascularization with mean change in score from 2.13±0.34 preoperatively to 0.09±0.30 postoperatively. Pinguecula excision and conjunctival autograft using fibrin glue is an effective and safe method to remove Pinguecula for cosmetic purposes. The method presented facilitated regression of vascularization.

  • One-year outcome of argon laser photocoagulation of Pinguecula.
    Cornea, 2013
    Co-Authors: Seong Joon Ahn, Kyunghoon Shin, Won Ryang Wee, Mee Kum Kim, Jiwon Kwon
    Abstract:

    Purpose:To determine the efficacy and safety of argon laser photocoagulation of Pinguecula by evaluating 1-year outcomes.Methods:Twenty-one eyes of 15 patients treated with argon laser photocoagulation (photocoagulation group) and 23 eyes of 16 patients treated with surgical excision (excision group