The Experts below are selected from a list of 360 Experts worldwide ranked by ideXlab platform
Julie A Theriot - One of the best experts on this subject based on the ideXlab platform.
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microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell target interactions
Nature Communications, 2020Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (<50 nm) accuracy using conventional confocal microscopy. We introduce a reference-free computational method allowing inference of traction forces with high sensitivity directly from the particle shape. We illustrate the potential of this approach by revealing subcellular force patterns throughout phagocytic engulfment and force dynamics in the cytotoxic T-cell immunological synapse. This strategy can readily be adapted for studying cellular forces in a wide range of applications. Traction force microscopy is an effective method for measuring cellular forces but it is limited by Planar Geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.
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superresolved microparticle traction force microscopy reveals subcellular force patterns in immune cell target interactions
bioRxiv, 2019Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Yifan Wang, Wei Cai, Julie A TheriotAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish a straightforward batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles, which can also be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
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superresolved and reference free microparticle traction force microscopy mp tfm reveals the complexity of the mechanical interaction in phagocytosis
bioRxiv, 2018Co-Authors: Daan Vorselen, Matthew J Footer, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish an accessible batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles. In addition, they can be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
Daan Vorselen - One of the best experts on this subject based on the ideXlab platform.
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microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell target interactions
Nature Communications, 2020Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (<50 nm) accuracy using conventional confocal microscopy. We introduce a reference-free computational method allowing inference of traction forces with high sensitivity directly from the particle shape. We illustrate the potential of this approach by revealing subcellular force patterns throughout phagocytic engulfment and force dynamics in the cytotoxic T-cell immunological synapse. This strategy can readily be adapted for studying cellular forces in a wide range of applications. Traction force microscopy is an effective method for measuring cellular forces but it is limited by Planar Geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.
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superresolved microparticle traction force microscopy reveals subcellular force patterns in immune cell target interactions
bioRxiv, 2019Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Yifan Wang, Wei Cai, Julie A TheriotAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish a straightforward batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles, which can also be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
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superresolved and reference free microparticle traction force microscopy mp tfm reveals the complexity of the mechanical interaction in phagocytosis
bioRxiv, 2018Co-Authors: Daan Vorselen, Matthew J Footer, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish an accessible batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles. In addition, they can be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
Matthew J Footer - One of the best experts on this subject based on the ideXlab platform.
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microparticle traction force microscopy reveals subcellular force exertion patterns in immune cell target interactions
Nature Communications, 2020Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing spatial force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy for studying cellular interactions. We establish a straightforward batch approach for synthesizing uniform, deformable and tuneable hydrogel particles, which can also be easily derivatized. The 3D shape of such particles can be resolved with superresolution (<50 nm) accuracy using conventional confocal microscopy. We introduce a reference-free computational method allowing inference of traction forces with high sensitivity directly from the particle shape. We illustrate the potential of this approach by revealing subcellular force patterns throughout phagocytic engulfment and force dynamics in the cytotoxic T-cell immunological synapse. This strategy can readily be adapted for studying cellular forces in a wide range of applications. Traction force microscopy is an effective method for measuring cellular forces but it is limited by Planar Geometry. Here the authors develop a facile method to produce deformable hydrogel particles and a reference-free computational method to resolve surface traction forces from particle shape deformation.
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superresolved microparticle traction force microscopy reveals subcellular force patterns in immune cell target interactions
bioRxiv, 2019Co-Authors: Daan Vorselen, Miguel M De Jesus, Matthew J Footer, Morgan Huse, Pavak K Shah, Yifan Wang, Wei Cai, Julie A TheriotAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish a straightforward batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles, which can also be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
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superresolved and reference free microparticle traction force microscopy mp tfm reveals the complexity of the mechanical interaction in phagocytosis
bioRxiv, 2018Co-Authors: Daan Vorselen, Matthew J Footer, Julie A Theriot, Yifan WangAbstract:Force exertion is an integral part of cellular behavior. Traction force microscopy (TFM) has been instrumental for studying such forces, providing both spatial and directional force measurements at subcellular resolution. However, the applications of classical TFM are restricted by the typical Planar Geometry. Here, we develop a particle-based force sensing strategy, specifically designed for studying ligand-dependent cellular interactions. We establish an accessible batch approach for synthesizing highly uniform, deformable and tunable hydrogel particles. In addition, they can be easily derivatized to trigger specific cellular behavior. The 3D shape of such particles can be resolved with superresolution (
Mark A Anastasio - One of the best experts on this subject based on the ideXlab platform.
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numerical investigation of the effects of shear waves in transcranial photoacoustic tomography with a Planar Geometry
Journal of Biomedical Optics, 2012Co-Authors: Robert W Schoonover, Lihong V Wang, Mark A AnastasioAbstract:Using a recently developed reconstruction method for photoacoustic tomography (PAT) valid for a Planar measurement Geometry parallel to a layered medium, we investigate the effects of shear wave propagation in the solid layer upon the ability to estimate Fourier components of the object. We examine this ability as a function of the thickness of the layer supporting shear waves as well as of the incidence angle of the field in the planewave representation. Examples are used to demonstrate the importance of accounting for shear waves in transcranial PAT. Error measures are introduced to quantify the error found when omitting shear waves from the forward model in PAT.
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analysis of the role of shear waves in transcranial photoacoustic tomography with a Planar Geometry
Proceedings of SPIE, 2012Co-Authors: Robert W Schoonover, Lihong V Wang, Mark A AnastasioAbstract:We report on an investigation of the role of shear waves in transcranial PAT brain imaging. Using a recently developed PAT image reconstruction method for use with layered media, we quantify the extent to which accounting for shear waves in the reconstruction method can improve image quality. The effects of shear waves propagating in the solid layer on the ability to estimate Fourier components of the object are investigated as a function of the thickness of the layer supporting shear waves as well as the incidence angle of the field in the planewave representation. These results clarify the role of shear waves in transcranial PAT image formation and indicate that further research is warranted to develop reconstruction algorithms that account for shear waves.
David P Pappas - One of the best experts on this subject based on the ideXlab platform.
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radiation suppressed superconducting quantum bit in a Planar Geometry
Applied Physics Letters, 2013Co-Authors: Martin Sandberg, Michael Vissers, Thomas A Ohki, Jiansong Gao, Jose Aumentado, Martin Weides, David P PappasAbstract:We present a superconducting transmon qubit circuit design based on large, coPlanar capacitor plates and a microstrip resonator. The microstrip Geometry, with the ground plane on the back, enhances access to the circuit for state preparation and measurement relative to other designs. The device is fabricated on a silicon substrate using low loss, stoichiometric titanium nitride for the capacitor plates and a single small aluminium/aluminium-oxide/aluminium junction. We observe relaxation and coherence times of 11.7 ± 0.2 μs and 9.6 ± 0.5 μs, respectively, using spin echo. Calculations show that the close proximity of the superconducting back-plane has the added advantage of suppressing the otherwise high radiation loss of the qubit.
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long lived radiation suppressed superconducting quantum bit in a Planar Geometry
arXiv: Superconductivity, 2012Co-Authors: Martin Sandberg, Michael Vissers, Thomas A Ohki, Jiansong Gao, Jose Aumentado, Martin Weides, David P PappasAbstract:We present a superconducting qubit design that is fabricated in a 2D Geometry over a superconducting ground plane to enhance the lifetime. The qubit is coupled to a microstrip resonator for readout. The circuit is fabricated on a silicon substrate using low loss, stoichiometric titanium nitride for capacitor pads and small, shadow-evaporated aluminum/aluminum-oxide junctions. We observe qubit relaxation and coherence times ($T_1$ and $T_2$) of 11.7 $\pm$ 0.2 $\mu$s and 8.7 $\pm$ 0.3 $\mu$s, respectively. Calculations show that the proximity of the superconducting plane suppresses the otherwise high radiation loss of the qubit. A significant increase in $T_1$ is projected for a reduced qubit-to-superconducting plane separation.
