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Nianzhi Jiao - One of the best experts on this subject based on the ideXlab platform.
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profiling Planctomycetales diversity with reference to anammox related bacteria in a south china sea deep sea sediment
Marine Ecology, 2008Co-Authors: Nianzhi JiaoAbstract:A systematic assessment of Planctomycetales diversity in a South China Sea, deep-sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla-46-F/1392-R showed that the Planctomycetales existed within a limited range of sediment depths (≤ 5 m), and had a decreasing trend in diversity with increasing depth. The majority of the retrieved Pla-46-F/1392-R sequences belonged to Pirellula-related Planctomycetales, and two sequences retrieved from the 0.1-m layer (GenBank accession numbers: DQ996944 and DQ996945) shared the same anammox-related signature oligonucleotides and were closely related to commonly recognized anammox organisms. To identify new anammox-related biomarkers, three primer sets were designed for amplifying the fragments of hydroxylamine oxidoreductase and S-adenosylmethionine radical enzyme genes, but no related sequences were found. Our multiple 16S rRNA gene primer sets (Journal of Rapid Methods and Automation in Microbiology, 2008, in press) revealed even an higher diversity of Planctomycetales in the 0.1-m layer of the sediment, especially at genus level. Our data profiled the distribution pattern of Planctomycetales diversity along sediment depths, and provided molecular evidence for the existence of anammox-related bacteria in a new location, which broadens our understanding of Planctomycetales diversity in deep sea sediments.
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Profiling Planctomycetales diversity with reference to anammox-related bacteria in a South China Sea, deep-sea sediment
Marine Ecology, 2008Co-Authors: Qinglong Shu, Nianzhi JiaoAbstract:A systematic assessment of Planctomycetales diversity in a South China Sea, deep-sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla-46-F/1392-R showed that the Planctomycetales existed within a limited range of sediment depths (
Svetlana N. Dedysh - One of the best experts on this subject based on the ideXlab platform.
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Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Irina S. Kulichevskaya, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Jaap S. Sinninghe Damsté, Svetlana N. DedyshAbstract:Four novel strains of budding bacteria, designated MOB10T, PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0-6.2) and at temperatures between 4 and 33 degrees C (optimum growth at 20-26 degrees C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C16 : 0, C18 : 1omega9c and C18 : 2omega6c,12c. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8-59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10T, PO2, MPL1015 and BG32 were members of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Isosphaera, exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete Isosphaera pallida and 95-95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, 'Nostocoida limicola' III. However, compared with 'Nostocoida limicola' III and Isosphaera pallida, the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order Planctomycetales, for which the name Singulisphaera acidiphila gen. nov., sp. nov. is proposed. The type strain of Singulisphaera acidiphila is MOB10T (=ATCC BAA-1392T =VKM B-2454T =DSM 18658T).
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Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Irina S. Kulichevskaya, Jaap S. Sinninghe Damsté, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Svetlana E. Belova, G A Zavarzin, W Irene C Rijpstra, Svetlana N. DedyshAbstract:Three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic Sphagnum-dominated boreal wetlands of northern Russia and were designated strains MPL7T, MOB77 and SB2. The presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. These isolates are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4.2 and 7.5 (with an optimum at pH 5.0-6.2) and at temperatures between 4 and 32 degrees C (optimum 15-26 degrees C). The major fatty acids are C16:0 and C16:1omega7c; the major quinone is MK-6. The G+C content of the DNA is 54.4-56.5 mol%. Strains MPL7T, MOB77 and SB2 possess nearly identical 16S rRNA gene sequences and belong to the planctomycete lineage defined by the genus Planctomyces, being most closely related to Planctomyces limnophilus DSM 3776T (86.9-87.1% sequence similarity). However, strain MPL7T showed only 28% DNA-DNA hybridization with P. limnophilus DSM 3776T. Compared with currently described members of the genus Planctomyces, the isolates from northern wetlands do not form long and distinctive stalks, have greater tolerance of acidic conditions and low temperatures, are more sensitive to NaCl, lack pigmentation and degrade a wider range of biopolymers. The data therefore suggest that strains MPL7T, MOB77 and SB2 represent a novel genus and species, for which the name Schlesneria paludicola gen. nov., sp. nov., is proposed. Strain MPL7T (=ATCC BAA-1393T=VKM B-2452T) is the type strain of Schlesneria paludicola.
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Genome Analysis of Fimbriiglobus ruber SP5T, a Planctomycete with Confirmed Chitinolytic Capability
Applied and Environmental Microbiology, 2018Co-Authors: Nikolai V. Ravin, Irina S. Kulichevskaya, Andrey L. Rakitin, Anastasia A. Ivanova, Alexey V. Beletsky, Andrey V. Mardanov, Svetlana N. DedyshAbstract:Members of the bacterial order Planctomycetales have often been observed in associations with Crustacea. The ability to degrade chitin, however, has never been reported for any of the cultured planctomycetes, although utilization of N-acetylglucosamine (GlcNAc) as sole carbon and nitrogen source is well-recognized for these bacteria. Here, we demonstrate the chitinolytic capability of a member of the family Gemmataceae , Fimbriiglobus ruber SP5 T , which was isolated from a peat bog. As revealed by metatranscriptomic analysis of chitin-amended peat, the pool of 16S rRNA reads from F. ruber increased in response to chitin availability. Strain SP5 T displayed only a weak growth on amorphous chitin as a sole source of carbon but grew well with chitin as a source of nitrogen. The genome of F. ruber SP5 T is 12.364 Mb in size and is the largest among all currently determined planctomycete genomes. It encodes several enzymes putatively involved in chitin degradation including two chitinases affiliated with the glycoside hydrolase (GH) family GH18, GH20 family β- N -acetylglucosaminidase as well as the complete set of enzymes required for utilization of GlcNAc. The gene encoding one of the predicted chitinases was expressed in E. coli and the endochitinase activity of the recombinant enzyme was confirmed. The genome also contains genes required for the assembly of type IV pili, which may be used to adhere to chitin and possibly other biopolymers. The ability to use chitin as a source of nitrogen is of special importance for planctomycetes that inhabit N-depleted ombrotrophic wetlands. Importance Planctomycetes represent an important part of the microbial community in Sphagnum -dominated peatlands but their potential functions in these ecosystems remain poorly understood. This study reports the presence of chitinolytic potential in one of the recently described peat-inhabiting members of the family Gemmataceae , Fimbriiglobus ruber SP5 T . This planctomycete uses chitin, a major constituent of fungal cell walls and exoskeletons of peat-inhabiting arthropods, as a source of nitrogen in N-depleted ombrotrophic Sphagnum -dominated peatlands. This study reports the chitin-degrading capability of representatives of the order Planctomycetales .
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A novel filamentous planctomycete of the Isosphaera-Singulisphaera group isolated from a Sphagnum peat bog
Microbiology, 2012Co-Authors: Irina S. Kulichevskaya, Anastasia A. Ivanova, Svetlana E. Belova, Svetlana N. DedyshAbstract:Planctomycetes are common inhabitants of northern wetlands. A significant proportion of these bacteria revealed in peat with the Planctomycetes-specific probes PLA46 and PLA886 is represented by filamentous forms which have not been cultured under laboratory conditions. In the present work, one of such organisms was isolated from a Sphagnum peat bog in a monoculture. The organism had large spherical cells assembled in long filaments. It could grow only in associations with bacterial satellites; attempts to isolate it in pure culture were unsuccessful. The organism was identified by PCR amplification, cloning, and subsequent analysis of its 16S rRNA gene fragment. Comparative sequence analysis revealed its affiliation with the Isosphaera-Singulisphaera group within the order Planctomycetales. The nucleotide sequence of the 16S rRNA gene of the new organism exhibited 94–96% similarity to those of the unicellular peat-inhabiting planctomycete Singulisphaera acidiphila and uncharacterized filamentous planctomycete “Nostocoida limicola III” from activated sludge. The new planctomycete utilized heteropolysaccharides of microbial origin as growth substrates and could grow at the low pH and temperatures typical of the northern wetlands.
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Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands.
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Irina S. Kulichevskaya, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Jaap S. Sinninghe Damsté, Svetlana N. DedyshAbstract:Four novel strains of budding bacteria, designated MOB10T, PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0–6.2) and at temperatures between 4 and 33 °C (optimum growth at 20–26 °C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C16 : 0, C18 : 1 ω9c and C18 : 2 ω6c,12c. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8–59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10T, PO2, MPL1015 and BG32 were members of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Isosphaera, exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete Isosphaera pallida and 95–95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, ‘Nostocoida limicola’ III. However, compared with ‘Nostocoida limicola’ III and Isosphaera pallida, the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order Planctomycetales, for which the name Singulisphaera acidiphila gen. nov., sp. nov. is proposed. The type strain of Singulisphaera acidiphila is MOB10T (=ATCC BAA-1392T =VKM B-2454T =DSM 18658T).
Irina S. Kulichevskaya - One of the best experts on this subject based on the ideXlab platform.
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Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Irina S. Kulichevskaya, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Jaap S. Sinninghe Damsté, Svetlana N. DedyshAbstract:Four novel strains of budding bacteria, designated MOB10T, PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0-6.2) and at temperatures between 4 and 33 degrees C (optimum growth at 20-26 degrees C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C16 : 0, C18 : 1omega9c and C18 : 2omega6c,12c. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8-59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10T, PO2, MPL1015 and BG32 were members of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Isosphaera, exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete Isosphaera pallida and 95-95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, 'Nostocoida limicola' III. However, compared with 'Nostocoida limicola' III and Isosphaera pallida, the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order Planctomycetales, for which the name Singulisphaera acidiphila gen. nov., sp. nov. is proposed. The type strain of Singulisphaera acidiphila is MOB10T (=ATCC BAA-1392T =VKM B-2454T =DSM 18658T).
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Schlesneria paludicola gen. nov., sp. nov., the first acidophilic member of the order Planctomycetales, from Sphagnum-dominated boreal wetlands.
International journal of systematic and evolutionary microbiology, 2020Co-Authors: Irina S. Kulichevskaya, Jaap S. Sinninghe Damsté, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Svetlana E. Belova, G A Zavarzin, W Irene C Rijpstra, Svetlana N. DedyshAbstract:Three strains of budding, ellipsoid-shaped and rosette-forming bacteria were isolated from acidic Sphagnum-dominated boreal wetlands of northern Russia and were designated strains MPL7T, MOB77 and SB2. The presence of crateriform pits and numerous fibrillar appendages on the cell surface and an unusual spur-like projection on one pole of the cell indicated a planctomycete morphotype. These isolates are moderately acidophilic, mesophilic organisms capable of growth at pH values between 4.2 and 7.5 (with an optimum at pH 5.0-6.2) and at temperatures between 4 and 32 degrees C (optimum 15-26 degrees C). The major fatty acids are C16:0 and C16:1omega7c; the major quinone is MK-6. The G+C content of the DNA is 54.4-56.5 mol%. Strains MPL7T, MOB77 and SB2 possess nearly identical 16S rRNA gene sequences and belong to the planctomycete lineage defined by the genus Planctomyces, being most closely related to Planctomyces limnophilus DSM 3776T (86.9-87.1% sequence similarity). However, strain MPL7T showed only 28% DNA-DNA hybridization with P. limnophilus DSM 3776T. Compared with currently described members of the genus Planctomyces, the isolates from northern wetlands do not form long and distinctive stalks, have greater tolerance of acidic conditions and low temperatures, are more sensitive to NaCl, lack pigmentation and degrade a wider range of biopolymers. The data therefore suggest that strains MPL7T, MOB77 and SB2 represent a novel genus and species, for which the name Schlesneria paludicola gen. nov., sp. nov., is proposed. Strain MPL7T (=ATCC BAA-1393T=VKM B-2452T) is the type strain of Schlesneria paludicola.
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Genome Analysis of Fimbriiglobus ruber SP5T, a Planctomycete with Confirmed Chitinolytic Capability
Applied and Environmental Microbiology, 2018Co-Authors: Nikolai V. Ravin, Irina S. Kulichevskaya, Andrey L. Rakitin, Anastasia A. Ivanova, Alexey V. Beletsky, Andrey V. Mardanov, Svetlana N. DedyshAbstract:Members of the bacterial order Planctomycetales have often been observed in associations with Crustacea. The ability to degrade chitin, however, has never been reported for any of the cultured planctomycetes, although utilization of N-acetylglucosamine (GlcNAc) as sole carbon and nitrogen source is well-recognized for these bacteria. Here, we demonstrate the chitinolytic capability of a member of the family Gemmataceae , Fimbriiglobus ruber SP5 T , which was isolated from a peat bog. As revealed by metatranscriptomic analysis of chitin-amended peat, the pool of 16S rRNA reads from F. ruber increased in response to chitin availability. Strain SP5 T displayed only a weak growth on amorphous chitin as a sole source of carbon but grew well with chitin as a source of nitrogen. The genome of F. ruber SP5 T is 12.364 Mb in size and is the largest among all currently determined planctomycete genomes. It encodes several enzymes putatively involved in chitin degradation including two chitinases affiliated with the glycoside hydrolase (GH) family GH18, GH20 family β- N -acetylglucosaminidase as well as the complete set of enzymes required for utilization of GlcNAc. The gene encoding one of the predicted chitinases was expressed in E. coli and the endochitinase activity of the recombinant enzyme was confirmed. The genome also contains genes required for the assembly of type IV pili, which may be used to adhere to chitin and possibly other biopolymers. The ability to use chitin as a source of nitrogen is of special importance for planctomycetes that inhabit N-depleted ombrotrophic wetlands. Importance Planctomycetes represent an important part of the microbial community in Sphagnum -dominated peatlands but their potential functions in these ecosystems remain poorly understood. This study reports the presence of chitinolytic potential in one of the recently described peat-inhabiting members of the family Gemmataceae , Fimbriiglobus ruber SP5 T . This planctomycete uses chitin, a major constituent of fungal cell walls and exoskeletons of peat-inhabiting arthropods, as a source of nitrogen in N-depleted ombrotrophic Sphagnum -dominated peatlands. This study reports the chitin-degrading capability of representatives of the order Planctomycetales .
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A novel filamentous planctomycete of the Isosphaera-Singulisphaera group isolated from a Sphagnum peat bog
Microbiology, 2012Co-Authors: Irina S. Kulichevskaya, Anastasia A. Ivanova, Svetlana E. Belova, Svetlana N. DedyshAbstract:Planctomycetes are common inhabitants of northern wetlands. A significant proportion of these bacteria revealed in peat with the Planctomycetes-specific probes PLA46 and PLA886 is represented by filamentous forms which have not been cultured under laboratory conditions. In the present work, one of such organisms was isolated from a Sphagnum peat bog in a monoculture. The organism had large spherical cells assembled in long filaments. It could grow only in associations with bacterial satellites; attempts to isolate it in pure culture were unsuccessful. The organism was identified by PCR amplification, cloning, and subsequent analysis of its 16S rRNA gene fragment. Comparative sequence analysis revealed its affiliation with the Isosphaera-Singulisphaera group within the order Planctomycetales. The nucleotide sequence of the 16S rRNA gene of the new organism exhibited 94–96% similarity to those of the unicellular peat-inhabiting planctomycete Singulisphaera acidiphila and uncharacterized filamentous planctomycete “Nostocoida limicola III” from activated sludge. The new planctomycete utilized heteropolysaccharides of microbial origin as growth substrates and could grow at the low pH and temperatures typical of the northern wetlands.
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Singulisphaera acidiphila gen. nov., sp. nov., a non-filamentous, Isosphaera-like planctomycete from acidic northern wetlands.
International Journal of Systematic and Evolutionary Microbiology, 2008Co-Authors: Irina S. Kulichevskaya, Anastasia O. Ivanova, Olga Baulina, Paul L. E. Bodelier, Jaap S. Sinninghe Damsté, Svetlana N. DedyshAbstract:Four novel strains of budding bacteria, designated MOB10T, PO2, MPL1015 and BG32, were isolated from acidic wetlands of northern Russia. Cells of these four strains were aerobic, non-motile spheres that occurred singly or in shapeless aggregates and attached to surfaces by means of a holdfast material. The isolates were moderately acidophilic, mesophilic organisms capable of growth between pH 4.2 and 7.5 (optimum growth at pH 5.0–6.2) and at temperatures between 4 and 33 °C (optimum growth at 20–26 °C). The strains possessed a complex intracellular membrane system that compartmentalized the cells. The major fatty acids were C16 : 0, C18 : 1 ω9c and C18 : 2 ω6c,12c. The major quinone was menaquinone-6 (MK-6). The G+C content of the DNA was 57.8–59.9 mol%. 16S rRNA gene sequence analysis showed that strains MOB10T, PO2, MPL1015 and BG32 were members of the order Planctomycetales and belonged to a phylogenetic lineage defined by the genus Isosphaera, exhibiting 90 % sequence similarity to the type strain of the thermophilic planctomycete Isosphaera pallida and 95–95.5 % sequence similarity to a taxonomically uncharacterized group of filamentous bacteria from activated sludge, ‘Nostocoida limicola’ III. However, compared with ‘Nostocoida limicola’ III and Isosphaera pallida, the new isolates from acidic wetlands were non-filamentous, unpigmented bacteria, which possessed highly distinctive phospholipid fatty acid profiles and were capable of growth and of degrading several biopolymers under acidic, microaerobic and cold conditions. The data suggest that the four isolates should be considered as representing a novel species of a new genus of the order Planctomycetales, for which the name Singulisphaera acidiphila gen. nov., sp. nov. is proposed. The type strain of Singulisphaera acidiphila is MOB10T (=ATCC BAA-1392T =VKM B-2454T =DSM 18658T).
Qinglong Shu - One of the best experts on this subject based on the ideXlab platform.
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Profiling Planctomycetales diversity with reference to anammox-related bacteria in a South China Sea, deep-sea sediment
Marine Ecology, 2008Co-Authors: Qinglong Shu, Nianzhi JiaoAbstract:A systematic assessment of Planctomycetales diversity in a South China Sea, deep-sea sediment (1657 m) was conducted using the 16S rRNA gene analysis approach. PCR amplification of the samples from seven sediment layers (0.1, 1, 3, 5, 7, 9 and 11 m below the surface sediment) using the primer set Pla-46-F/1392-R showed that the Planctomycetales existed within a limited range of sediment depths (
Mike S. M. Jetten - One of the best experts on this subject based on the ideXlab platform.
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Chapter 16 – Anammox
Biology of the Nitrogen Cycle, 2020Co-Authors: Huub J. M. Op Den Camp, Mike S. M. Jetten, Marc StrousAbstract:Publisher Summary This chapter describes the microbiological investigation of anaerobic NH4 oxidation. During the last 10 years, the anammox process evolved from a largely unexplored part of the biological N cycle to general textbook knowledge. In the last few years, it has become clear that anammox is a major player in the global N cycle. The principle of selective enrichment, as first developed by Beyerinck, demands that to isolate a particular microorganism, the organism first has to be provided with what might be described as the complement of its ecological niche. It shows the exponential increase in N load and the resulting accumulation of anammox biomass. The persisting stable and strongly selective conditions of the sequencing batch reactor (SBR) led to a high degree of enrichment. The SBR approach made possible the determination of several important physiological parameters, such as the biomass yield and the maximum specific growth rate. Because the common ancestors of the bacterial phyla cannot be discriminated phylogenetically, it follows that chemolithoautotrophy can be expected in every bacterial phylotaxonomic position. The order Planctomycetales is one of the many currently defined (more than 70) distinct divisions that form the domain bacteria. On the basis of 16S rRNA phylogeny, biochemical composition, and morphology, the genera Planctomyces (floating fungus), Pirellula (pear-shaped), Gemmata (bud-bearer), and Isosphaera (spheres of equal size) have been placed in a distinct order, Planctomycetales, and family, Planctomycetaceae. Recently, a detailed study of members of the genus Pirellula resulted in the definition of two new genera, Rhodopirellula and Blastopirellula.
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Caulifigura coniformis gen. nov., sp. nov., a novel member of the family Planctomycetaceae isolated from a red biofilm sampled in a hydrothermal area
Antonie van Leeuwenhoek, 2020Co-Authors: Nicolai Kallscheuer, Mike S. M. Jetten, Stijn H. Peeters, Sandra Wiegand, Mareike Jogler, Anja Heuer, Christian Boedeker, Manfred Rohde, Christian JoglerAbstract:Pan44^T, a novel strain belonging to the phylum Planctomycetes , was isolated from a red biofilm in a hydrothermal area close to the island Panarea in the Tyrrhenian Sea north of Sicily, Italy. The strain forms white colonies on solid medium and displays the following characteristics: cell division by budding, formation of rosettes, presence of matrix or fimbriae and long stalks. The cell surface has an interesting and characteristic texture made up of triangles and rectangles, which leads to a pine cone-like morphology of the strain. Strain Pan44^T is mesophilic (temperature optimum 26 °C), slightly alkaliphilic (pH optimum 8.0), aerobic and heterotrophic. The strain has a genome size of 6.76 Mb with a G + C content of 63.2%. Phylogenetically, the strain is a member of the family Planctomycetaceae , order Planctomycetales , class Planctomycetia . Our analysis supports delineation of strain Pan44^T from all known genera in this family, hence, we propose to assign it to a novel species within a novel genus, for which we propose the name Caulifigura coniformis gen. nov., sp. nov., represented by Pan44^T (DSM 29405^T = LMG 29788^T) as the type strain.
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Calycomorphotria hydatis gen. nov., sp. nov., a novel species in the family Planctomycetaceae with conspicuous subcellular structures
Antonie van Leeuwenhoek, 2020Co-Authors: Torsten Schubert, Mike S. M. Jetten, Stijn H. Peeters, Sandra Wiegand, Nicolai Kallscheuer, Mareike Jogler, Anja Heuer, Christian Boedeker, Manfred Rohde, Christian JoglerAbstract:A novel strain belonging to the family Planctomycetaceae , designated V22^T, was isolated from sediment of a seawater fish tank in Braunschweig, Germany. The isolate forms pink colonies on solid medium and displays common characteristics of planctomycetal strains, such as division by budding, formation of rosettes, a condensed nucleoid and presence of crateriform structures and fimbriae. Unusual invaginations of the cytoplasmic membrane and filamentous putative cytoskeletal elements were observed in thin sections analysed by transmission electron microscopy. Strain V22^T is an aerobic heterotroph showing optimal growth at 30 °C and pH 8.5. During laboratory cultivations, strain V22^T reached generation times of 10 h (maximal growth rate of 0.069 h^−1). Its genome has a size of 5.2 Mb and a G + C content of 54.9%. Phylogenetically, the strain represents a novel genus and species in the family Planctomycetaceae , order Planctomycetales , class Planctomycetia . We propose the name Calycomorphotria hydatis gen. nov., sp. nov. for the novel taxon, represented by the type strain V22^T (DSM 29767^T = LMG 29080^T).
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Description of Polystyrenella longa gen. nov., sp. nov., isolated from polystyrene particles incubated in the Baltic Sea
Antonie van Leeuwenhoek, 2020Co-Authors: Stijn H. Peeters, Mike S. M. Jetten, Sandra Wiegand, Nicolai Kallscheuer, Mareike Jogler, Anja Heuer, Christian Boedeker, Manfred Rohde, Christian JoglerAbstract:Planctomycetes occur in almost all aquatic ecosystems on earth. They have a remarkable cell biology, and members of the orders Planctomycetales and Pirellulales feature cell division by polar budding, perform a lifestyle switch from sessile to motile cells and have an enlarged periplasmic space. Here, we characterise a novel planctomycetal strain, Pla110^T, isolated from the surface of polystyrene particles incubated in the Baltic Sea. After phylogenetic analysis, the strain could be placed in the family Planctomycetaceae . Strain Pla110^T performs cell division by budding, has crateriform structures and grows in aggregates or rosettes. The strain is a chemoheterotroph, grows under mesophilic and neutrophilic conditions, and exhibited a doubling time of 21 h. Based on our phylogenetic and morphological characterisation, strain Pla110^T (DSM 103387^T = LMG 29693^T) is concluded to represent a novel species belonging to a novel genus, for which we propose the name Polystyrenella longa gen. nov., sp. nov.
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Description of three bacterial strains belonging to the new genus Novipirellula gen. nov., reclassificiation of Rhodopirellula rosea and Rhodopirellula caenicola and readjustment of the genus threshold of the phylogenetic marker rpoB for Planctomycet
Antonie van Leeuwenhoek, 2019Co-Authors: Nicolai Kallscheuer, Mike S. M. Jetten, Stijn H. Peeters, Sandra Wiegand, Mareike Jogler, Anja Heuer, Christian Boedeker, Manfred Rohde, Patrick Rast, Christian JoglerAbstract:Access to axenic cultures of Planctomycetes is crucial for further investigating their complex lifestyle, uncommon cell biology and primary and secondary metabolism. As a contribution to achieve this goal in the future, we here describe three strains belonging to the novel genus Novipirellula gen. nov. The strains were isolated from biotic and abiotic surfaces in the Baltic Sea and from the island Heligoland in the North Sea. Colony colours range from white to light pink. Cells are acorn-shaped and grew optimally at neutral pH and temperatures between 27 and 30 °C. Phylogenetic analyses revealed that the isolated strains represent three novel species belonging to a new genus, Novipirellula gen. nov. Beyond that, our analysis suggests that Rhodopirellula rosea LHWP3^T, Rhodopirellula caenicola YM26-125^T and Rhodopirellula maiorica SM1 are also members of this novel genus. Splitting the current genus Rhodopirellula into a more strictly defined genus Rhodopirellula and Novipirellula also allowed readjusting the genus threshold value for the gene rpoB , encoding the RNA polymerase β-subunit, which is used as phylogenetic marker for Planctomycetales . A threshold range of 75.5–78% identity of the analysed partial rpoB sequence turned out to be reliable for differentiation of genera within the family Planctomycetaceae .