The Experts below are selected from a list of 315 Experts worldwide ranked by ideXlab platform
Guanghong Zhou - One of the best experts on this subject based on the ideXlab platform.
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effect of pre emulsification of Plant Lipid treated by pulsed ultrasound on the functional properties of chicken breast myofibrillar protein composite gel
Food Research International, 2014Co-Authors: Yingying Zhao, Peng Wang, Ke Li, Zhuangli Kang, Xinglian Xu, Guanghong ZhouAbstract:Abstract To explore novel methods for reducing the saturated fatty acid content in meat products, pre-emulsified Plant Lipids prepared using pulsed ultrasound were used to replace animal fat. Chicken breast myofibrillar protein (MP)–soybean oil emulsion composite gels (SMG) were prepared from 3% chicken breast MP with 27.5% soybean oil incorporated as pre-emulsification with 0.5% sodium caseinate (0.6 M NaCl and 50 mM phosphate solution, pH 6.25). One-factor-at-a-time experiments were carried out to investigate the effect of different ultrasound treatment times on the texture [rheology and texture profile analysis (TPA)], water- and fat-binding (WFB) capacities, and microstructural properties of SMG. Rheological tests showed that ultrasound-treated samples formed a more viscoelastic gel than the control. WFB and textural properties were also significantly improved by pulsed ultrasound ( P 72 °C and G″ 72 °C were highly correlated ( P
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Effect of pre-emulsification of Plant Lipid treated by pulsed ultrasound on the functional properties of chicken breast myofibrillar protein composite gel
Food Research International, 2014Co-Authors: Yingying Zhao, Peng Wang, Ke Li, Zhuangli Kang, Xinglian Xu, Guanghong ZhouAbstract:Abstract To explore novel methods for reducing the saturated fatty acid content in meat products, pre-emulsified Plant Lipids prepared using pulsed ultrasound were used to replace animal fat. Chicken breast myofibrillar protein (MP)–soybean oil emulsion composite gels (SMG) were prepared from 3% chicken breast MP with 27.5% soybean oil incorporated as pre-emulsification with 0.5% sodium caseinate (0.6 M NaCl and 50 mM phosphate solution, pH 6.25). One-factor-at-a-time experiments were carried out to investigate the effect of different ultrasound treatment times on the texture [rheology and texture profile analysis (TPA)], water- and fat-binding (WFB) capacities, and microstructural properties of SMG. Rheological tests showed that ultrasound-treated samples formed a more viscoelastic gel than the control. WFB and textural properties were also significantly improved by pulsed ultrasound (P
Christoph Benning - One of the best experts on this subject based on the ideXlab platform.
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WITHDRAWN: Functional diversity of glyceroLipid acylhydrolases in Plant metabolism and physiology
Progress in Lipid Research, 2019Co-Authors: Kun Wang, Timothy P Durrett, Christoph BenningAbstract:Abstract Most current knowledge about Plant Lipid metabolism has focused on the biosynthesis of Lipids and their transport between different organelles. However, Lipid composition changes during development and in response to environmental cues often go beyond adjustments of Lipid biosynthesis. When Lipids have to be removed to adjust the extent of membranes during down regulation of photosynthesis, or Lipid composition has to be adjusted to alter the biophysical properties of membranes, or Lipid derived chemical signals have to be produced, Lipid-degrading enzymes come into play. This review focuses on glyceroLipid acylhydrolases that remove acyl groups from glyceroLipids and will highlight their roles in Lipid remodeling and Lipid-derived signal generation. One emerging theme is that these enzymes are involved in the dynamic movement of acyl groups through different Lipid pools, for example from polar membrane Lipids to neutral Lipids sequestered in Lipid droplets during de novo triacylglycerol synthesis. Another example of acyl group sequestration in the form of triacylglycerols in Lipid droplets is membrane Lipid remodeling in response to abiotic stresses. Fatty acids released for membrane Lipids can also give rise to potent signaling molecules and acylhydrolases are therefore often the first step in initiating the formation of these Lipid signals.
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functional diversity of glyceroLipid acylhydrolases in Plant metabolism and physiology
Progress in Lipid Research, 2019Co-Authors: Kun Wang, Timothy P Durrett, Christoph BenningAbstract:Abstract Most current knowledge about Plant Lipid metabolism has focused on the biosynthesis of Lipids and their transport between different organelles. However, Lipid composition changes during development and in response to environmental cues often go beyond adjustments of Lipid biosynthesis. When Lipids have to be removed to adjust the extent of membranes during down regulation of photosynthesis, or Lipid composition has to be adjusted to alter the biophysical properties of membranes, or Lipid derived chemical signals have to be produced, Lipid-degrading enzymes come into play. This review focuses on glyceroLipid acylhydrolases that remove acyl groups from glyceroLipids and will highlight their roles in Lipid remodeling and Lipid-derived signal generation. One emerging theme is that these enzymes are involved in the dynamic movement of acyl groups through different Lipid pools, for example from polar membrane Lipids to neutral Lipids sequestered in Lipid droplets during de novo triacylglycerol synthesis. Another example of acyl group sequestration in the form of triacylglycerols in Lipid droplets is membrane Lipid remodeling in response to abiotic stresses. Fatty acids released for membrane Lipids can also give rise to potent signaling molecules and acylhydrolases are therefore often the first step in initiating the formation of these Lipid signals.
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cellular organization and regulation of Plant glyceroLipid metabolism
Plant and Cell Physiology, 2019Co-Authors: Anastasiya Lavell, Christoph BenningAbstract:: Great strides have been made in understanding how membranes and Lipid droplets are formed and maintained in land Plants, yet much more is to be learned given the complexity of Plant Lipid metabolism. A complicating factor is the multi-organellar presence of biosynthetic enzymes and unique compositional requirements of different membrane systems. This necessitates a rich network of transporters and transport mechanisms that supply fatty acids, membrane Lipids and storage Lipids to their final cellular destination. Though we know a large number of the biosynthetic enzymes involved in Lipid biosynthesis and a few transport proteins, the regulatory mechanisms, in particular, coordinating expression and/or activity of the majority remain yet to be described. Plants undergoing stress alter their membranes' compositions, and Lipids such as phosphatidic acid have been implicated in stress signaling. Additionally, Lipid metabolism in chloroplasts supplies precursors for jasmonic acid (JA) biosynthesis, and perturbations in Lipid homeostasis has consequences on JA signaling. In this review, several aspects of Plant Lipid metabolism are discussed that are currently under investigation: cellular transport of Lipids, regulation of Lipid biosynthesis, roles of Lipids in stress signaling, and lastly the structural and oligomeric states of Lipid enzymes.
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The Plant Lipidome in human and environmental health
Science, 2016Co-Authors: Patrick J Horn, Christoph BenningAbstract:Lipids and oils derived from Plant and algal photosynthesis constitute much of human daily caloric intake and provide the basis for high-energy bioproducts, chemical feedstocks for countless applications, and even fossil fuels over geological time scales. Sustainable production of high-energy compounds from Plants is essential to preserving fossil fuel sources and ensuring the well-being of future generations. As a result of progress in basic research on Plant and algal Lipid metabolism, in combination with advances in synthetic biology, we can now tailor Plant Lipids for desirable biological, physical, and chemical properties. We highlight recent advances in Plant Lipid translational biology and discuss untapped areas of research that might expand the application of Plant Lipids.
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Lipid trafficking in Plant cells
Traffic, 2014Co-Authors: Anna K Hurlock, Kun Wang, Rebecca L Roston, Christoph BenningAbstract:Plant cells contain unique organelles such as chloroplasts with an extensive photosynthetic membrane. In addition, specialized epidermal cells produce an extracellular cuticle composed primarily of Lipids, and storage cells accumulate large amounts of storage Lipids. As Lipid assembly is associated only with discrete membranes or organelles, there is a need for extensive Lipid trafficking within Plant cells, more so in specialized cells and sometimes also in response to changing environmental conditions such as phosphate deprivation. Because of the complexity of Plant Lipid metabolism and the inherent recalcitrance of membrane Lipid transporters, the mechanisms of Lipid transport within Plant cells are not yet fully understood. Recently, several new proteins have been implicated in different aspects of Plant Lipid trafficking. While these proteins provide only first insights into limited aspects of Lipid transport phenomena in Plant cells, they represent exciting opportunities for further studies.
Yingying Zhao - One of the best experts on this subject based on the ideXlab platform.
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effect of pre emulsification of Plant Lipid treated by pulsed ultrasound on the functional properties of chicken breast myofibrillar protein composite gel
Food Research International, 2014Co-Authors: Yingying Zhao, Peng Wang, Ke Li, Zhuangli Kang, Xinglian Xu, Guanghong ZhouAbstract:Abstract To explore novel methods for reducing the saturated fatty acid content in meat products, pre-emulsified Plant Lipids prepared using pulsed ultrasound were used to replace animal fat. Chicken breast myofibrillar protein (MP)–soybean oil emulsion composite gels (SMG) were prepared from 3% chicken breast MP with 27.5% soybean oil incorporated as pre-emulsification with 0.5% sodium caseinate (0.6 M NaCl and 50 mM phosphate solution, pH 6.25). One-factor-at-a-time experiments were carried out to investigate the effect of different ultrasound treatment times on the texture [rheology and texture profile analysis (TPA)], water- and fat-binding (WFB) capacities, and microstructural properties of SMG. Rheological tests showed that ultrasound-treated samples formed a more viscoelastic gel than the control. WFB and textural properties were also significantly improved by pulsed ultrasound ( P 72 °C and G″ 72 °C were highly correlated ( P
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Effect of pre-emulsification of Plant Lipid treated by pulsed ultrasound on the functional properties of chicken breast myofibrillar protein composite gel
Food Research International, 2014Co-Authors: Yingying Zhao, Peng Wang, Ke Li, Zhuangli Kang, Xinglian Xu, Guanghong ZhouAbstract:Abstract To explore novel methods for reducing the saturated fatty acid content in meat products, pre-emulsified Plant Lipids prepared using pulsed ultrasound were used to replace animal fat. Chicken breast myofibrillar protein (MP)–soybean oil emulsion composite gels (SMG) were prepared from 3% chicken breast MP with 27.5% soybean oil incorporated as pre-emulsification with 0.5% sodium caseinate (0.6 M NaCl and 50 mM phosphate solution, pH 6.25). One-factor-at-a-time experiments were carried out to investigate the effect of different ultrasound treatment times on the texture [rheology and texture profile analysis (TPA)], water- and fat-binding (WFB) capacities, and microstructural properties of SMG. Rheological tests showed that ultrasound-treated samples formed a more viscoelastic gel than the control. WFB and textural properties were also significantly improved by pulsed ultrasound (P
Kent D. Chapman - One of the best experts on this subject based on the ideXlab platform.
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Lipidomics in situ insights into Plant Lipid metabolism from high resolution spatial maps of metabolites
Progress in Lipid Research, 2014Co-Authors: Patrick J Horn, Kent D. ChapmanAbstract:The emergence of ‘omics’ technologies (i.e. genomics, proteomics, metabolomics, etc.) have revealed new avenues for exploring Plant metabolism through data-rich experimentation and integration of complementary methodologies. Over the past decade, the Lipidomics field has benefited from advances in instrumentation, especially mass spectrometry (MS)-based approaches that are well-suited for detailed Lipid analysis. The broad classification of what constitutes a Lipid lends itself to a structurally diverse range of molecules that contribute to a variety of biological processes in Plants including membrane structure and transport, primary and secondary metabolism, abiotic and biotic stress tolerances, extracellular and intracellular signaling, and energy-rich storage of carbon. Progress in these research areas has been advanced in part through approaches analyzing chemical compositions of Lipids in extracts from cells, tissues and/or whole organisms (e.g. shotgun Lipidomics), and through visualization approaches primarily through microscopy-based methodologies (e.g. fluorescence, bright field, electron microscopy, etc.). While these techniques on their own provide rich biochemical and biological information, coordinated analyses of the complexity of Lipid composition with the localization of these Lipids at a high spatial resolution will help to develop a new level of understanding of Lipid metabolism within the context of tissue/cellular compartmentation. This review will elaborate on recent advances of one such approach – mass spectrometry imaging (MSI) – that integrates in situ visualization with chemical-based Lipidomics. We will illustrate, with an emphasis on oilseed Lipid metabolism, how MS imaging can provide new insights and questions related to the spatial compartmentation of Lipid metabolism in Plants. Further it will be apparent that this MS imaging approach has broad application in Plant metabolic research well beyond that of triacylglycerol biosynthesis in oilseeds.
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Highlights of recent progress in Plant Lipid research.
Plant Physiology and Biochemistry, 2009Co-Authors: René Lessire, Edgar B Cahoon, Kent D. Chapman, John M. Dyer, Peter J. Eastmond, E. HeinzAbstract:Raw fossil material reserves are not inexhaustible and as prices continue to raise it is necessary to find new sources of alternative and renewable energy. Oils from oleaginous field crops (sunflower and rape) with properties close to those of fossil fuel could constitute an alternative source of energy for the production of raw materials. This is the context in which the 18th International Symposium on Plant Lipids (ISPL) was held in Bordeaux from 20th to 25th July 2008 at ''La CiteMondiale''. The 18th ISPL gathered 270 researchers from 33 countries. Sixty nine oral communications and 136 posters were presented during the 12 sessions of the Symposium. The sessions have covered all the different aspects of the Plant Lipid field including: Surface Lipids: suberin, cutin and waxes, Fatty acids, GlyceroLipids, Plant Lipids as renewable sources of energy, Seed oils and bioengineering of metabolic pathways, Lipid catabolism, Models for Lipid studies: lower Plants, micro-organisms and others, Modifications of proteins by Lipids, SphingoLipids, sterols and isoprenoids, Lipid signaling and Plant stress responses, Lipid traf- ficking and membrane dynamics, New methods and technologies: functional Lipidomics, fluxome, modelling. During the ISPL 2008 Bordeaux, important and new information was reported in the different fields. A selection of these results is presented here.
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2009 Plant Lipids: Structure, Metabolism & Function Gordon Research Conference - February 1- 6 ,2009
2009Co-Authors: Kent D. ChapmanAbstract:The Gordon Research Conference on 'Plant Lipids: Structure, Metabolism and Function' has been instituted to accelerate research productivity in the field of Plant Lipids. This conference will facilitate wide dissemination of research breakthroughs, support recruitment of young scientists to the field of Plant Lipid metabolism and encourage broad participation of the Plant Lipid community in guiding future directions for research in Plant Lipids. This conference will build upon the strengths of the successful, previous biannual meetings of the National Plant Lipid Cooperative (www.PlantLipids.org) that began in 1993, but will reflect a broader scope of topics to include the biochemistry, cell biology, metabolic regulation, and signaling functions of Plant acyl Lipids. Most importantly, this conference also will serve as a physical focal point for the interaction of the Plant Lipid research community. Applications to attend this conference will be open to all researchers interested in Plant Lipids and will provide a venue for the presentation of the latest research results, networking opportunities for young scientists, and a forum for the development and exchange of useful Lipid resources and new ideas. By bringing together senior- and junior-level scientists involved in Plant Lipid metabolism, a broad range of insights will be shared andmore » the community of Plant Lipid researchers will function more as a network of vested partners. This is important for the vitality of the research community and for the perceived value that will encourage conference attendance into the future.« less
Enrique Martínez-force - One of the best experts on this subject based on the ideXlab platform.
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Genetic engineering of castor bean (Ricinus communis)
2019Co-Authors: A.j. Sánchez-Álvarez, Antonio Javier Moreno-pérez, Rafael Garcés Mancheño, Enrique Martínez-force, Noemí Ruiz-lópez, Joaquín J. SalasAbstract:Plant Lipids: Structure, Metabolism and Function Gordon Research Conference Integrating Novel Genomic, Biophysical, Computational and Visualization Approaches to Advance Plant Lipid Research January 27 - February 1, 2019
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Effect of a mutagenized acyl-ACP thioesterase FATA allele from sunflower with improved activity in tobacco leaves and Arabidopsis seeds
Planta, 2014Co-Authors: Antonio Javier Moreno-pérez, Ian A Graham, Joaquín J. Salas, Mónica Venegas-calerón, Fabián E. Vaistij, Tony R. Larson, Rafael Garcés, Enrique Martínez-forceAbstract:The substrate specificity of the acyl–acyl carrier protein (ACP) thioesterases significantly determines the type of fatty acids that are exported from plastids. Thus, designing acyl-ACP thioesterases with different substrate specificities or kinetic properties would be of interest for Plant Lipid biotechnology to produce oils enriched in specialty fatty acids. In the present work, the FatA thioesterase from Helianthus annuu s was used to test the impact of changes in the amino acids present in the binding pocket on substrate specificity and catalytic efficiency. Amongst all the mutated enzymes studied, Q215W was especially interesting as it had higher specificity towards saturated acyl-ACP substrates and higher catalytic efficiency compared to wild-type H. annuus FatA. Null, wild type and high-efficiency alleles were transiently expressed in tobacco leaves to check their effect on Lipid biosynthesis. Expression of active FatA thioesterases altered the composition of leaf triacylglycerols but did not alter total Lipid content. However, the expression of the wild type and the high-efficiency alleles in Arabidopsis thaliana transgenic seeds resulted in a strong reduction in oil content and an increase in total saturated fatty acid content. The role and influence of acyl-ACP thioesterases in Plant metabolism and their possible applications in Lipid biotechnology are discussed.