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Ingo Potrykus - One of the best experts on this subject based on the ideXlab platform.

  • Arabidopsis thaliana: protocol for Plant Regeneration from protoplasts.
    Plant cell reports, 1991
    Co-Authors: Hans Karesch, Roland Bilang, Ingo Potrykus
    Abstract:

    We report a protocol for Plant Regeneration from leaf protoplasts of Arabidopsis thaliana ecotype Zurich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in Plant Regeneration from protoplast-derived clones is highly variable. In the hands of one of us (H.K.) average shoot Regeneration frequency (% of calli regenerating at least one shoot) was ca. 60% and average Plant Regeneration frequency (% of calli yielding fertile Plants in soil) was ca. 40%.

Leslaw Przywara - One of the best experts on this subject based on the ideXlab platform.

  • Two pathways of Plant Regeneration in wheat anther culture
    Plant Cell Tissue and Organ Culture, 2003
    Co-Authors: Robert Konieczny, A.z. Czaplicki, Hieronim Golczyk, Leslaw Przywara
    Abstract:

    The anthers of 10 Polish winter wheat (Triticum aestivum L.) cultivars were used for the induction of androgenesis and Plant Regeneration. The highest rate of callus induction (9.1%) and green Plant production (0.8%) was obtained with the cultivar Apollo that was chosen for histological analysis. The first androgenic division was symmetrical and occurred after 3 weeks of culture. Further divisions of newly formed cells gave rise to multicellular structures which followed two developmental pathways: callus production or direct embryo formation. Plant Regeneration was observed in both pathways. Chromosome counting of Plantlets regenerated showed that haploid metaphases 2n=3x=21 were the most frequent.

Pawan K Jaiwal - One of the best experts on this subject based on the ideXlab platform.

  • Plant Regeneration from mature embryo of commercial indian bread wheat triticum aestivum l cultivars
    Physiology and Molecular Biology of Plants, 2012
    Co-Authors: Sanjay Singh Parmar, Manish Sainger, Darshna Chaudhary, Pawan K Jaiwal
    Abstract:

    A simple, efficient, reproducible and comparatively genotype-independent in vitro Plant Regeneration protocol was developed for ten commercial Indian bread wheat cultivars using mature embryos as the exPlants. Three different auxins and five different combinations of growth regulators in a modified Murashige and Skoog’s basal medium were assessed for their effect on callus induction and Plant Regeneration, respectively, in a high yielding and widely grown cultivar, PBW-343. The optimized conditions were further evaluated with nine other commercial cultivars. A simple novel approach of physical isolation of regenerable calli from non regenerable structures during the early callus phase was used to improve Plant Regeneration. Callus induced on 2.0 mg-1 2,4-dichlorophenoxyacetic acid (2,4-D) showed a Regeneration frequency of 86 % with 7.5 shoots per exPlants on hormone-free medium. A considerable improvement in the Regeneration frequency (up to 97 %) and the average of shoots (19 shoots per exPlants) was obtained with a combination of thidiazuron (TDZ) and 2,4-D.

Hans Karesch - One of the best experts on this subject based on the ideXlab platform.

  • Arabidopsis thaliana: protocol for Plant Regeneration from protoplasts.
    Plant cell reports, 1991
    Co-Authors: Hans Karesch, Roland Bilang, Ingo Potrykus
    Abstract:

    We report a protocol for Plant Regeneration from leaf protoplasts of Arabidopsis thaliana ecotype Zurich. The protocol has been established in 1988 and has since been in routine use in our laboratory. Whereas recovery of proliferating protoplast-derived clones is routine, the success in Plant Regeneration from protoplast-derived clones is highly variable. In the hands of one of us (H.K.) average shoot Regeneration frequency (% of calli regenerating at least one shoot) was ca. 60% and average Plant Regeneration frequency (% of calli yielding fertile Plants in soil) was ca. 40%.

Satbir Singh Gosal - One of the best experts on this subject based on the ideXlab platform.

  • In vitro direct Plant Regeneration protocol for tomato genotypes
    Indian Journal of Horticulture, 2013
    Co-Authors: Ruma Devi, Dhaliwal, Satbir Singh Gosal
    Abstract:

    A high frequency direct Plant Regeneration method was developed for four tomato genotypes, viz., Castle Rock, Punjab Upma, VFN-8 and IPA-3. Cotyledons of 8-day-old in vitro grown seedlings of the four genotypes were excised and cultured onto Murashige an Skoog (MS) medium supplemented with different concentrations and combinations of BAP and kinetin. The frequency of Plant Regeneration and number of shoots per exPlant was influenced by the genotype and concentration of growth regulators. Among the genotypes tested, IPA-3 recoded the maximum Plant Regeneration (95.14%) followed by Punjab Upma (93.13%), Castle Rock (90.02%) and VFN-8 (82.09%). Among the different media compositions tried maximum average Plant Regeneration (90.14%) and number of shoots (6.17) were obtained using MS medium supplemented with BAP 2.0 mg l−1 and kinetin 1.0 mg l−1. With either decrease or increase from the optimum level, decline in percent Plant Regeneration as well as number of shoots per exPlant was recorded. The shoots when attained approximately 2 cm height were transferred to the half-strength MS medium, which led to simultaneous shoot elongation as well as root formation. All the shoots recorded 100% rooting and were subsequently transferred to poly bags containing FYM: sand:soil (1:1:1) and kept in glasshouse for acclimatization.

  • Influence of antibiotic cefotaxime on somatic embryogenesis and Plant Regeneration in indica rice.
    Biotechnology journal, 2006
    Co-Authors: Deepinder Grewal, Raman Gill, Satbir Singh Gosal
    Abstract:

    An antibiotic, cefotaxime (Omnatax™) has been found to promote somatic embryogenesis and subsequent Plant Regeneration in vitro in indica-type basmati rice cultures. Response was highly genotype specific. The number, mass and morphology of the calli formed on the scutellar tissues were dependent on the growth medium (with or without cefotaxime). The embryogenic nature of nodular calli was confirmed through histological analysis and their Plant Regeneration ability. The calli of variety Pusa basmati 1 grown on medium supplemented with cefotaxime (100 mg/L) exhibited up to 70.5% Plant Regeneration as compared to control (51.51%). Plants regenerated from emryogenic calli were phenotypically normal and identical to seed-derived Plants and exhibited normal fertility. A limited humidity and an optimal aeration of the culture tubes further enhanced the frequency of somatic embryogenesis and Plant Regeneration.