The Experts below are selected from a list of 4368 Experts worldwide ranked by ideXlab platform

Manuela E. Gomes - One of the best experts on this subject based on the ideXlab platform.

  • Cellulose nanocrystals of variable sulfation degrees can sequester specific Platelet Lysate-derived biomolecules to modulate stem cell response.
    Chemical communications (Cambridge England), 2020
    Co-Authors: Bárbara B. Mendes, Rui L. Reis, Manuel Gómez-florit, Hugo Osório, Adriana Vilaça, Rui M. A. Domingues, Manuela E. Gomes
    Abstract:

    The surface chemistry of cellulose nanocrystals was engineered to show variable sulfation degrees, which was exploited to modulate Platelet Lysate-derived biomolecule sequestration and presentation. The protein coronas developed on CNC surfaces were characterized and it was demonstrated how they promote different signaling effects on human adipose-derived stem cell behavior.

  • Platelet Lysate cell-laden hydrogel-coated suture threads for tendon repair
    2018
    Co-Authors: Raquel Costa-almeida, Rui M. A. Domingues, Rui L. Reis, Isabel Calejo, Manuela E. Gomes
    Abstract:

    Tendon injuries constitute a major healthcare burden owing to the limited healing ability of these tissues and the poor clinical outcomes of surgical repair treatments. Recent advances in tendon tissue engineering (TTE) strategies, particularly through the use of biotextile technologies, hold great promise toward the generation of artificial living tendon constructs. We have previously developed a braided construct based on suture threads coated with gelMA:alginate hydrogel encapsulating human tendon cells. These cell-laden composite fibers enabled the replication of cell and tissue-level properties simultaneously. Based on this concept, in this study we explored the use of Platelet Lysate (PL), a pool of supra-physiological concentrations of growth factors (GFs), to generate a hydrogel layer, which is envisioned to act as a depot of therapeutic factors to induce tenogenic differentiation of encapsulated human adipose stem cells (hASCs). For this purpose, commercially available suture threads were first e...

  • Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology
    ACS Biomaterials Science & Engineering, 2017
    Co-Authors: Pedro S. Babo, Rui L. Reis, Ricardo Leandro Pires, Lívia Santos, Albina R. Franco, Fernando Rodrigues, Isabel B. Leonor, Manuela E. Gomes
    Abstract:

    The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However, an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated hyaluronic acid (me-HA) and incorporating Platelet Lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, deg...

  • Engineering Enriched Microenvironments with Gradients of Platelet Lysate in Hydrogel Fibers.
    Biomacromolecules, 2016
    Co-Authors: Vítor E. Santo, Rui L. Reis, João F. Mano, Pedro S. Babo, Miguel Amador, Cláudia Correia, Bárbara Cunha, Daniela F. Coutinho, Nuno M. Neves, Manuela E. Gomes
    Abstract:

    Gradients of physical and chemical cues are characteristic of specific tissue microenvironments and contribute toward morphogenesis and tissue regeneration upon injury. Recent advances on microfluidics and hydrogel manipulation raised the possibility of generating biomimetic biomaterials enriched with bioactive factors and encapsulating cells following designs specifically tailored for a target application. The novelty of this work relies on the combination of methacrylated gellan gum (MeGG) with Platelet Lysate (PL), aiming to generate novel advanced 3D PL-enriched photo-cross-linkable hydrogels and overcoming the lack of adhesion sites provided by the native MeGG hydrogels. This combination takes advantage of the availability, enriched growth factor composition, and potential autologous application of PL while simultaneously preserving the ability provided by MeGG to tailor mechanical properties, protein release kinetics, and shape of the construct according to the desired goal. Incorporation of PL in t...

  • Platelet Lysate-based pro-angiogenic nanocoatings.
    Acta biomaterialia, 2015
    Co-Authors: Sara M. Oliveira, Rogério P. Pirraco, Alexandra P. Marques, Vítor E. Santo, Manuela E. Gomes, Rui L. Reis, João F. Mano
    Abstract:

    Abstract Human Platelet Lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with Platelet Lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.

Rui L. Reis - One of the best experts on this subject based on the ideXlab platform.

  • Cellulose nanocrystals of variable sulfation degrees can sequester specific Platelet Lysate-derived biomolecules to modulate stem cell response.
    Chemical communications (Cambridge England), 2020
    Co-Authors: Bárbara B. Mendes, Rui L. Reis, Manuel Gómez-florit, Hugo Osório, Adriana Vilaça, Rui M. A. Domingues, Manuela E. Gomes
    Abstract:

    The surface chemistry of cellulose nanocrystals was engineered to show variable sulfation degrees, which was exploited to modulate Platelet Lysate-derived biomolecule sequestration and presentation. The protein coronas developed on CNC surfaces were characterized and it was demonstrated how they promote different signaling effects on human adipose-derived stem cell behavior.

  • Platelet Lysate cell-laden hydrogel-coated suture threads for tendon repair
    2018
    Co-Authors: Raquel Costa-almeida, Rui M. A. Domingues, Rui L. Reis, Isabel Calejo, Manuela E. Gomes
    Abstract:

    Tendon injuries constitute a major healthcare burden owing to the limited healing ability of these tissues and the poor clinical outcomes of surgical repair treatments. Recent advances in tendon tissue engineering (TTE) strategies, particularly through the use of biotextile technologies, hold great promise toward the generation of artificial living tendon constructs. We have previously developed a braided construct based on suture threads coated with gelMA:alginate hydrogel encapsulating human tendon cells. These cell-laden composite fibers enabled the replication of cell and tissue-level properties simultaneously. Based on this concept, in this study we explored the use of Platelet Lysate (PL), a pool of supra-physiological concentrations of growth factors (GFs), to generate a hydrogel layer, which is envisioned to act as a depot of therapeutic factors to induce tenogenic differentiation of encapsulated human adipose stem cells (hASCs). For this purpose, commercially available suture threads were first e...

  • Platelet Lysate-Loaded Photocrosslinkable Hyaluronic Acid Hydrogels for Periodontal Endogenous Regenerative Technology
    ACS Biomaterials Science & Engineering, 2017
    Co-Authors: Pedro S. Babo, Rui L. Reis, Ricardo Leandro Pires, Lívia Santos, Albina R. Franco, Fernando Rodrigues, Isabel B. Leonor, Manuela E. Gomes
    Abstract:

    The integrity and function of the periodontium can be compromised by traumatic injuries or periodontitis. Currently available clinical therapies are able to stop the progression of periodontitis and allow the healing of periodontal tissue. However, an optimal strategy capable of restoring the anatomy and functionality of the lost periodontal tissue is still to be achieved. Herein is proposed the development of an injectable hydrogel system able to release a growth factors and cells to the periodontal defect. This injectable system is based on a photocrosslinkable hydrogel, prepared from methacrylated hyaluronic acid (me-HA) and incorporating Platelet Lysate (PL). The delivery of growth factors and cells in situ is expected to enhance regeneration of the periodontium. Various formulations of me-HA containing increasing PL concentrations were studied for achieving the formation of stable photocrosslinkable hydrogels. The produced hydrogels were subsequently characterized to assess mechanical properties, deg...

  • Engineering Enriched Microenvironments with Gradients of Platelet Lysate in Hydrogel Fibers.
    Biomacromolecules, 2016
    Co-Authors: Vítor E. Santo, Rui L. Reis, João F. Mano, Pedro S. Babo, Miguel Amador, Cláudia Correia, Bárbara Cunha, Daniela F. Coutinho, Nuno M. Neves, Manuela E. Gomes
    Abstract:

    Gradients of physical and chemical cues are characteristic of specific tissue microenvironments and contribute toward morphogenesis and tissue regeneration upon injury. Recent advances on microfluidics and hydrogel manipulation raised the possibility of generating biomimetic biomaterials enriched with bioactive factors and encapsulating cells following designs specifically tailored for a target application. The novelty of this work relies on the combination of methacrylated gellan gum (MeGG) with Platelet Lysate (PL), aiming to generate novel advanced 3D PL-enriched photo-cross-linkable hydrogels and overcoming the lack of adhesion sites provided by the native MeGG hydrogels. This combination takes advantage of the availability, enriched growth factor composition, and potential autologous application of PL while simultaneously preserving the ability provided by MeGG to tailor mechanical properties, protein release kinetics, and shape of the construct according to the desired goal. Incorporation of PL in t...

  • Platelet Lysate-based pro-angiogenic nanocoatings.
    Acta biomaterialia, 2015
    Co-Authors: Sara M. Oliveira, Rogério P. Pirraco, Alexandra P. Marques, Vítor E. Santo, Manuela E. Gomes, Rui L. Reis, João F. Mano
    Abstract:

    Abstract Human Platelet Lysate (PL) is a cost-effective and human source of autologous multiple and potent pro-angiogenic factors, such as vascular endothelial growth factor A (VEGF A), fibroblast growth factor b (FGF b) and angiopoietin-1. Nanocoatings previously characterized were prepared by layer-by-layer assembling incorporating PL with marine-origin polysaccharides and were shown to activate human umbilical vein endothelial cells (HUVECs). Within 20 h of incubation, the more sulfated coatings induced the HUVECS to the form tube-like structures accompanied by an increased expression of angiogenic-associated genes, such as angiopoietin-1 and VEGF A. This may be a cost-effective approach to modify 2D/3D constructs to instruct angiogenic cells towards the formation of neo-vascularization, driven by multiple and synergistic stimulations from the PL combined with sulfated polysaccharides. Statement of Significance The presence, or fast induction, of a stable and mature vasculature inside 3D constructs is crucial for new tissue formation and its viability. This has been one of the major tissue engineering challenges, limiting the dimensions of efficient tissue constructs. Many approaches based on cells, growth factors, 3D bioprinting and channel incorporation have been proposed. Herein, we explored a versatile technique, layer-by-layer assembling in combination with Platelet Lysate (PL), that is a cost-effective source of many potent pro-angiogenic proteins and growth factors. Results suggest that the combination of PL with sulfated polyelectrolytes might be used to introduce interfaces onto 2D/3D constructs with potential to induce the formation of cell-based tubular structures.

Giuseppina Sandri - One of the best experts on this subject based on the ideXlab platform.

  • Electrospun Gelatin–Chondroitin Sulfate Scaffolds Loaded with Platelet Lysate Promote Immature Cardiomyocyte Proliferation
    Polymers, 2018
    Co-Authors: Francesca Saporito, Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Claudia Del Fante, Barbara Vigani, Lorenzo Malavasi, Lauren D. Black, Franca Ferrari
    Abstract:

    The aim of the present work was the development of heart patches based on gelatin (G) and chondroitin sulfate (CS) to be used as implants to improve heart recovery after corrective surgery for critical congenital heart defects (CHD). Patches were prepared by means of electrospinning to obtain nanofibrous scaffolds and they were loaded with Platelet Lysate (PL) as a source of growth factors to further enhance the repair process. Scaffolds were characterized for morphology and mechanical properties and for the capability to support in vitro adhesion and proliferation of dermal fibroblasts in order to assess the system's general biocompatibility. Adhesion and proliferation of endothelial cells and cardiac cells (cardiomyocytes and cardiac fibroblasts from rat fetuses) onto PL-loaded patches was evaluated. Patches presented good elasticity and high stiffness suitable for in vivo adaptation to heart contraction. CS improved adhesion and proliferation of dermal fibroblasts, as proof of their biocompatibility. Moreover, they enhanced the adhesion and proliferation of endothelial cells, a crucial mediator of cardiac repair. Cell adhesion and proliferation could be related to elastic properties, which could favor cell motility. The presence of Platelet Lysate and CS was crucial for the adhesion and proliferation of cardiac cells and, in particular, of cardiomyocytes: G/CS scaffold embedded with PL appeared to selectively promote proliferation in cardiomyocytes but not cardiac fibroblasts. In conclusion, G/CS scaffold seems to be a promising system to assist myocardial-repair processes in young patient, preserving cardiomyocyte viability and preventing cardiac fibroblast proliferation, likely reducing subsequent uncontrolled collagen deposition by fibroblasts following repair.

  • electrospun gelatin chondroitin sulfate scaffolds loaded with Platelet Lysate promote immature cardiomyocyte proliferation
    Polymers, 2018
    Co-Authors: Francesca Saporito, Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Claudia Del Fante, Barbara Vigani, Lorenzo Malavasi, Lauren D. Black, Franca Ferrari
    Abstract:

    The aim of the present work was the development of heart patches based on gelatin (G) and chondroitin sulfate (CS) to be used as implants to improve heart recovery after corrective surgery for critical congenital heart defects (CHD). Patches were prepared by means of electrospinning to obtain nanofibrous scaffolds and they were loaded with Platelet Lysate (PL) as a source of growth factors to further enhance the repair process. Scaffolds were characterized for morphology and mechanical properties and for the capability to support in vitro adhesion and proliferation of dermal fibroblasts in order to assess the system's general biocompatibility. Adhesion and proliferation of endothelial cells and cardiac cells (cardiomyocytes and cardiac fibroblasts from rat fetuses) onto PL-loaded patches was evaluated. Patches presented good elasticity and high stiffness suitable for in vivo adaptation to heart contraction. CS improved adhesion and proliferation of dermal fibroblasts, as proof of their biocompatibility. Moreover, they enhanced the adhesion and proliferation of endothelial cells, a crucial mediator of cardiac repair. Cell adhesion and proliferation could be related to elastic properties, which could favor cell motility. The presence of Platelet Lysate and CS was crucial for the adhesion and proliferation of cardiac cells and, in particular, of cardiomyocytes: G/CS scaffold embedded with PL appeared to selectively promote proliferation in cardiomyocytes but not cardiac fibroblasts. In conclusion, G/CS scaffold seems to be a promising system to assist myocardial-repair processes in young patient, preserving cardiomyocyte viability and preventing cardiac fibroblast proliferation, likely reducing subsequent uncontrolled collagen deposition by fibroblasts following repair.

  • Association of Alpha Tocopherol and Ag Sulfadiazine Chitosan Oleate Nanocarriers in Bioactive Dressings Supporting Platelet Lysate Application to Skin Wounds
    Marine drugs, 2018
    Co-Authors: Maria Cristina Bonferoni, Giuseppina Sandri, Silvia Rossi, Claudia Del Fante, Cesare Perotti, Eleonora Dellera, Alessandro Invernizzi, Cinzia Boselli, Antonia Icaro Cornaglia, Barbara Vigani
    Abstract:

    Chitosan oleate was previously proposed to encapsulate in nanocarriers some poorly soluble molecules aimed to wound therapy, such as the anti-infective silver sulfadiazine, and the antioxidant α tocopherol. Because nanocarriers need a suitable formulation to be administered to wounds, in the present paper, these previously developed nanocarriers were loaded into freeze dried dressings based on chitosan glutamate. These were proposed as bioactive dressings aimed to support the application to wounds of Platelet Lysate, a hemoderivative rich in growth factors. The dressings were characterized for hydration capacity, morphological aspect, and rheological and mechanical behavior. Although chitosan oleate nanocarriers clearly decreased the mechanical properties of dressings, these remained compatible with handling and application to wounds. Preliminary studies in vitro on fibroblast cell cultures demonstrated good compatibility of Platelet Lysate with nanocarriers and bioactive dressings. An in vivo study on a murine wound model showed an accelerating wound healing effect for the bioactive dressing and its suitability as support of the Platelet Lysate application to wounds.

  • Platelet Lysate and chondroitin sulfate loaded contact lenses to heal corneal lesions.
    International journal of pharmaceutics, 2016
    Co-Authors: Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Alessio Delfino, Federica Riva, Antonia Icaro Cornaglia, Giorgio Marrubini, Giorgio Musitelli, Claudia Del Fante, Cesare Perotti
    Abstract:

    Hemoderivative tear substitutes contain various ephiteliotrophic factors, such as growth factors (GF), involved in ocular surface homeostasis without immunogenic properties. The aim of the present work was the loading of Platelet Lysate into contact lenses to improve the precorneal permanence of Platelet Lysate growth factors on the ocular surface to enhance the treatment of corneal lesions. To this purpose, chondroitin sulfate, a sulfated glycosaminoglycan, which is normally present in the extracellular matrix, was associated with Platelet Lysate. In fact, chondroitin sulfate is capable of electrostatic interaction with positively charged growth factors, in particular, with bFGF, IGF, VEGF, PDGF and TGF-β, resulting in their stabilization and reduced degradation in solution. In the present work, various types of commercially available contact lenses have been loaded with chondroitin sulfate or chondroitin sulfate in association with Platelet Lysate to achieve a release of growth factors directly onto the corneal surface lesions. One type of contact lenses (PureVision(®)) showed in vitro good proliferation properties towards corneal cells and were able to enhance cut closure in cornea constructs.

  • Platelet Lysate embedded scaffolds for skin regeneration.
    Expert opinion on drug delivery, 2014
    Co-Authors: Giuseppina Sandri, Maria Cristina Bonferoni, Silvia Rossi, Federica Riva, Franca Ferrari, Michela Mori, Marila Cervio, Ioannis Liakos, Athanassia Athanassiou, Francesca Saporito
    Abstract:

    Introduction: The work presents the development of acellular scaffolds extemporaneously embedded with Platelet Lysate (PL), as an innovative approach in the field of tissue regeneration/reparation. PL embedded scaffolds should have a tridimensional architecture to support cell migration and growth, in order to restore skin integrity. For this reason, chondroitin sulfate (CS) was associated with sodium alginate (SA) to prepare highly porous systems.Methods: The developed scaffolds were characterized for chemical stability to γ-radiation, morphology, hydration and mechanical properties. Moreover, the capability of fibroblasts and endothelial cells to populate the scaffold was evaluated by means of proliferation test 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and confocal laser scanning microscopy study. The scaffolds, not altered by sterilization, were characterized by limited swelling and high flexibility, by foam-like structure with bubbles that formed a high surface area and irreg...

Giorgio Soldani - One of the best experts on this subject based on the ideXlab platform.

  • bilayered fibrin based electrospun sprayed scaffold loaded with Platelet Lysate enhances wound healing in a diabetic mouse model
    Nanomaterials, 2020
    Co-Authors: Paola Losi, Tamer Al Kayal, Marianna Buscemi, Ilenia Foffa, Aida Cavallo, Giorgio Soldani
    Abstract:

    The present study examined the effects of a bilayered fibrin/poly(ether)urethane scaffold loaded with Platelet Lysate by a combination of electrospinning and spray, phase-inversion method for wound healing. In particular, the poly(ether)urethane layer was obtained using by a spray phase-inversion method and the fibrin fibers network were loaded with Platelet Lysate by electrospinning. The kinetics release and the bioactivity of growth factors released from Platelet Lysate-scaffold were investigated by ELISA and cell proliferation test using mouse fibroblasts, respectively. The in-vitro experiments demonstrated that a bilayered fibrin/poly(ether)urethane scaffold loaded with Platelet Lysate provides a sustained release of bioactive Platelet-derived growth factors. The effect of a bilayered fibrin/poly(ether)urethane scaffold loaded with Platelet Lysate on wound healing in diabetic mouse (db/db) was also investigated. The application of the scaffold on full-thickness skin wounds significantly accelerated wound closure at day 14 post-surgery when compared to scaffold without Platelet Lysates or commercially available polyurethane film, and at the same level of growth factor-loaded scaffold. Histological analysis demonstrated an increased re-epithelialization and collagen deposition in Platelet Lysate and growth factor loaded scaffolds. The ability of bilayered fibrin/poly(ether)urethane scaffold loaded with Platelet Lysate to promote in-vivo wound healing suggests its usefulness in clinical treatment of diabetic ulcers.

  • Healing effect of a fibrin-based scaffold loaded with Platelet Lysate in full-thickness skin wounds:
    Journal of Bioactive and Compatible Polymers, 2015
    Co-Authors: Paola Losi, Enrica Briganti, Elena Sanguinetti, Silvia Burchielli, Tamer Al Kayal, Giorgio Soldani
    Abstract:

    Chronic skin lesions are difficult to heal due to reduced levels and activity of endogenous growth factors. The Platelet Lysate, obtained by repeated freeze–thawing of Platelet-enriched blood samples, is an easily attainable source of a wide range of growth factors and bioactive mediators involved in tissue repair. In this study, a bio-synthetic scaffold composed of poly(ether)urethane–polydimethylsiloxane material and fibrin was developed for Platelet Lysate delivery to chronic skin wounds. The kinetics release and the bioactivity of growth factors released from Platelet Lysate–loaded poly(ether)urethane–polydimethylsiloxane–fibrin scaffold were investigated, respectively, by enzyme-linked immunosorbent assay and a cell proliferation test using human fibroblasts. The in vitro experiments demonstrated that the Platelet Lysate–loaded poly(ether)urethane–polydimethylsiloxane–fibrin scaffold provides a sustained release of Platelet derived growth factors. The cell growth in the presence of scaffold was compa...

  • effect of Platelet Lysate on human cells involved in different phases of wound healing
    PLOS ONE, 2013
    Co-Authors: Maria Chiara Barsotti, Paola Losi, Enrica Briganti, Elena Sanguinetti, Tamer Al Kayal, A Magera, Roberto Feriani, Rossella Di Stefano, Giorgio Soldani
    Abstract:

    Background Platelets are rich in mediators able to positively affect cell activity in wound healing. Aim of this study was to characterize the effect of different concentrations of human pooled allogeneic Platelet Lysate on human cells involved in the different phases of wound healing (inflammatory phase, angiogenesis, extracellular matrix secretion and epithelialization). Methodology/Principal Findings Platelet Lysate effect was studied on endothelial cells, monocytes, fibroblasts and keratinocytes, in terms of viability and proliferation, migration, angiogenesis, tissue repair pathway activation (ERK1/2) and inflammatory response evaluation (NFκB). Results were compared both with basal medium and with a positive control containing serum and growth factors. Platelet Lysate induced viability and proliferation at the highest concentrations tested (10% and 20% v/v). Whereas both Platelet Lysate concentrations increased cell migration, only 20% Platelet Lysate was able to significantly promote angiogenic activity (p<0.05 vs. control), comparably to the positive control. Both Platelet Lysate concentrations activated important inflammatory pathways such as ERK1/2 and NFκB with the same early kinetics, whereas the effect was different for later time-points. Conclusion/Significance These data suggest the possibility of using allogeneic Platelet Lysate as both an alternative to growth factors commonly used for cell culture and as a tool for clinical regenerative application for wound healing.

  • Effect of Platelet Lysate on human cells involved in different phases of wound healing.
    PloS one, 2013
    Co-Authors: Maria Chiara Barsotti, Paola Losi, Enrica Briganti, Elena Sanguinetti, Tamer Al Kayal, A Magera, Roberto Feriani, Rossella Di Stefano, Giorgio Soldani
    Abstract:

    Background Platelets are rich in mediators able to positively affect cell activity in wound healing. Aim of this study was to characterize the effect of different concentrations of human pooled allogeneic Platelet Lysate on human cells involved in the different phases of wound healing (inflammatory phase, angiogenesis, extracellular matrix secretion and epithelialization). Methodology/Principal Findings Platelet Lysate effect was studied on endothelial cells, monocytes, fibroblasts and keratinocytes, in terms of viability and proliferation, migration, angiogenesis, tissue repair pathway activation (ERK1/2) and inflammatory response evaluation (NFκB). Results were compared both with basal medium and with a positive control containing serum and growth factors. Platelet Lysate induced viability and proliferation at the highest concentrations tested (10% and 20% v/v). Whereas both Platelet Lysate concentrations increased cell migration, only 20% Platelet Lysate was able to significantly promote angiogenic activity (p

Katharina Schallmoser - One of the best experts on this subject based on the ideXlab platform.

  • Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human Platelet Lysate.
    Journal of translational medicine, 2019
    Co-Authors: Sandra Laner-plamberger, Dirk Strunk, Michaela Oeller, Cornelia Mrazek, Arnulf Hartl, Alina Sarah Sonderegger, Eva Rohde, Katharina Schallmoser
    Abstract:

    Background Innovative human stromal cell therapeutics require xeno-free culture conditions. Various formulations of human Platelet Lysate (HPL) are efficient alternatives for fetal bovine serum (FBS). However, a consistent lack of standardized manufacturing protocols and quality criteria hampers comparability of HPL-products. Aim of this study was to compare the biochemical composition of three differential HPL-preparations with FBS and to investigate their impact on stromal cell biology.

  • Human Platelet Lysate current standards and future developments
    Transfusion, 2019
    Co-Authors: Reinhard Henschler, Thierry Burnouf, Katharina Schallmoser, Christian Gabriel, Mickey Koh
    Abstract:

    A state-of-the-art workshop focused on the use of human Platelet Lysate (HPL) for cell therapy. The meeting established that HPL is used mainly as an adjunct material for ex vivo expansion of mesenchymal stem/progenitor cells (MSCs), where it is successfully used as a substitute for fetal bovine serum. HPL manufacturing as a cell expansion supplement is currently not yet uniformly standardized with regard to Platelet source and production methodology. There are very few reports of HPL preparations manufactured specifically for direct clinical use. There exists an urgent need for controlled clinical studies for HPL and for standardization of product definition. Workshop participants also stated a need for consensus minimum release criteria to allow for better product definition and to limit variability in performance. The increasing use of cell-based therapies including MSCs has led to an increasing demand for HPL, either produced in blood establishments or large-scale manufacture by biopharmaceutical companies. The use of pooled donor Platelets for HPL production may require the implementation of pathogen inactivation procedures and/or removal steps to improve the safety of advanced cell therapy products. There should also be a requirement for thorough risk assessments and risk mitigation steps, including the qualification of suppliers and identification of ingredients as well as meticulous monitoring of product quality and safety profiles. State-of-the-art regulatory approaches for HPL used for human cell propagation and PRP in direct clinical applications were reviewed.

  • human Platelet Lysate replacing fetal bovine serum as a gold standard for human cell propagation
    Biomaterials, 2016
    Co-Authors: Thierry Burnouf, Dirk Strunk, Katharina Schallmoser
    Abstract:

    The essential physiological role of Platelets in wound healing and tissue repair builds the rationale for the use of human Platelet derivatives in regenerative medicine. Abundant growth factors and cytokines stored in Platelet granules can be naturally released by thrombin activation and clotting or artificially by freeze/thaw-mediated Platelet lysis, sonication or chemical treatment. Human Platelet Lysate prepared by the various release strategies has been established as a suitable alternative to fetal bovine serum as culture medium supplement, enabling efficient propagation of human cells under animal serum-free conditions for a multiplicity of applications in advanced somatic cell therapy and tissue engineering. The rapidly increasing number of studies using Platelet derived products for inducing human cell proliferation and differentiation has also uncovered a considerable variability of human Platelet Lysate preparations which limits comparability of results. The main variations discussed herein encompass aspects of donor selection, preparation of the starting material, the possibility for pooling in plasma or additive solution, the implementation of pathogen inactivation and consideration of ABO blood groups, all of which can influence applicability. This review outlines the current knowledge about human Platelet Lysate as a powerful additive for human cell propagation and highlights its role as a prevailing supplement for human cell culture capable to replace animal serum in a growing spectrum of applications.

  • Mechanical fibrinogen-depletion supports heparin-free mesenchymal stem cell propagation in human Platelet Lysate
    Journal of translational medicine, 2015
    Co-Authors: Sandra Laner-plamberger, Thomas Lener, Doris Schmid, Doris Streif, Tina Salzer, Michaela Öller, Cornelia Hauser-kronberger, Thorsten Fischer, Volker R. Jacobs, Katharina Schallmoser
    Abstract:

    Background Pooled human Platelet Lysate (pHPL) is an efficient alternative to xenogenic supplements for ex vivo expansion of mesenchymal stem cells (MSCs) in clinical studies. Currently, porcine heparin is used in pHPL-supplemented medium to prevent clotting due to plasmatic coagulation factors. We therefore searched for an efficient and reproducible medium preparation method that avoids clot formation while omitting animal-derived heparin.

  • Generation of a pool of human Platelet Lysate and efficient use in cell culture.
    Methods in molecular biology (Clifton N.J.), 2012
    Co-Authors: Katharina Schallmoser, Dirk Strunk
    Abstract:

    Human Platelets represent a promising source of bioactive substances as growth factors not just for in vivo wound healing and tissue repair, but also for the expansion of human stem and progenitor cells in vitro. The replacement of fetal bovine serum (FBS) as a standard culture supplement by human Platelet-derived growth factors now allows for the GMP-compliant implementation of various cell therapeutics in the growing field of regenerative medicine.For this purpose a protocol for the preparation of human Platelet Lysate (HPL) by several freeze-thaw cycles has been developed, resulting in Platelet fragmentation and the release of stored growth factors. By pooling up to 15 U of HPL derived from individual blood donors, a virtually standardized product is achieved. The depletion of Platelet particles and fragments in a final centrifugation step reduces the risk of alloimmunization against Platelet antigens and the formation of aggregates in cell culture.The successful application of pooled human Platelet Lysate (pHPL) as a culture medium supplement for the ex vivo propagation of human mesenchymal stem/progenitor cells (MSPCs) and endothelial colony forming progenitor cells (ECFCs) indicates the feasibility of this animal serum-free source of growth factors. Further studies will evaluate efficacy and safety of pHPL.