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Hans-jürgen Busse - One of the best experts on this subject based on the ideXlab platform.
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novosphingobium lubricantis sp nov isolated from a coolant lubricant emulsion
International Journal of Systematic and Evolutionary Microbiology, 2018Co-Authors: Peter Kampfer, Hans-jürgen Busse, Stefanie P GlaeserAbstract:A yellow-pigmented, Gram-stain-negative, rod-shaped, non-spore-forming bacterium (strain KSS165-70T) was isolated from a coolant lubricant emulsion. The 16S rRNA gene sequence analysis of strain KSS165-70T showed high sequence similarity to the type strains of Novosphingobium subterraneum (98.1 %), Novosphingobium lentum (97.9 %) and Novosphingobium taihuense (97.8 %). Sequence similarities to type strains of all other Novosphingobium species were below 97.5 %. Ubiquinone Q-10 was detected as the major respiratory quinone. The predominant fatty acid C18 : 1ω7c and the typical 2-hydroxy fatty acid C14 : 0 2-OH were detected. The Polar Lipid profile contained the major Lipids diphosphatidylglycerol, phosphatedylethanolamine, sphingoglycoLipid, phosphatidylcholine and two unidentified phosphoLipids. The polyamine pattern contained the major compound spermidine. Characterization by 16S rRNA gene sequence analysis, physiological parameters, pigment analysis, and ubiquinone, Polar Lipid and fatty acid composition revealed that strain KSS165-70T represents a new species of the genus Novosphingobium . For this reason, we propose the name Novosphingobium lubricantis sp. nov. with the type strain KSS165-70T (=CIP 111490T=CCM 8814T).
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review of the taxonomy of the genus arthrobacter emendation of the genus arthrobacter sensu lato proposal to reclassify selected species of the genus arthrobacter in the novel genera glutamicibacter gen nov paeniglutamicibacter gen nov pseudoglutamic
International Journal of Systematic and Evolutionary Microbiology, 2016Co-Authors: Hans-jürgen BusseAbstract:In this paper, the taxonomy of the genus Arthrobacter is discussed, from its first description in 1947 to the present state. Emphasis is given to intrageneric phylogeny and chemotaxonomic characteristics, concentrating on quinone systems, peptidoglycan compositions and Polar Lipid profiles. Internal groups within the genus Arthrobacter indicated from homogeneous chemotaxonomic traits and corresponding to phylogenetic grouping and/or high 16S rRNA gene sequence similarities are highlighted. Furthermore, Polar Lipid profiles and quinone systems of selected species are shown, filling some gaps concerning these chemotaxonomic traits. Based on phylogenetic groupings, 16S rRNA gene sequence similarities and homogeneity in peptidoglycan types, quinone systems and Polar Lipid profiles, a description of the genus Arthrobacter sensu lato and an emended description of Arthrobacter roseus are provided. Furthermore, reclassifications of selected species of the genus Arthrobacter into novel genera are proposed, namely Glutamicibacter gen. nov. (nine species), Paeniglutamicibacter gen. nov. (six species), Pseudoglutamicibacter gen. nov. (two species), Paenarthrobacter gen. nov. (six species) and Pseudarthrobacter gen. nov. (ten species).
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sphingobacterium psychroaquaticum sp nov a psychrophilic bacterium isolated from lake michigan water
International Journal of Systematic and Evolutionary Microbiology, 2013Co-Authors: Richard A Albert, Nancy E Waas, Shawn C Pavlons, Jamie L Pearson, Laura M Ketelboeter, Ramon Rossellomora, Hans-jürgen BusseAbstract:A psychrophilic, Gram-negative bacterium, designated MOL-1T, was isolated from water of Lake Michigan. 16S rRNA gene sequence analysis revealed that the sequence of strain MOL-1T has sequence similarity of 95.6, 94.8, 94.3, 94.3, 94.2 and 93.9 %, respectively, to the 16S rRNA gene sequences of Sphingobacterium shayense HS39T, S. lactis WCC 4512T, S. composti T5-12T, S. daejeonense TR6-04T, S. bambusae IBFC2009T and S. alimentarium WCC 4521T. The major cellular fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (iso-C15 : 0 2-OH and/or C16 : 1ω7c). Menaquinone MK-7 is the predominant respiratory quinone, while sym-homospermidine is the predominant polyamine. The Polar Lipid profile is composed of the predominant Lipids phosphatidylethanolamine and unidentified Polar Lipid L2, with moderate amounts of unidentified Polar Lipids L1, L5 and L6 and unidentified aminophosphoLipids APL1 and APL2 and minor to trace amounts of unidentified Polar Lipids L3, L4, L7, L8, L9 and L10, unidentified phosphoLipid PL4 and unidentified aminophosphoLipid APL3. After molecular and phenotypic studies, including chemotaxonomic analyses, it was concluded that strain MOL-1T represents a novel Sphingobacterium species, for which the name Sphingobacterium psychroaquaticum sp. nov. is proposed. The type strain is MOL-1T ( = NRRL B-59232T = DSM 22418T).
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identification of a bacterial strain isolated from the liver of a laboratory mouse as microbacterium paraoxydans and emended description of the species microbacterium paraoxydans laffineur et al 2003
Indian Journal of Microbiology, 2008Co-Authors: Sandra Buczolits, Maria Valens, Ramon Rossellomora, Peter Schumann, Hans-jürgen BusseAbstract:A rod-shaped, Gram-positive bacterial strain, designated C57-33, was isolated from the liver of the laboratory mouse strain C57Bl/6J and characterised by a polyphasic approach. 16S rRNA gene sequence similarity placed strain C57-33 in the genus Microbacterium with Microbacterium paraoxydans CF36T as the next relative (99.9% sequence similarity). Major fatty acids ai-C15:0, i-C16:0 and ai-C17:0 and peptidoglycan type B2β with ornithine as the diagnostic cell-wall diamino acid and glycolyl residues were in agreement with the description of Microbacterium paraoxydans. The quinone system of C57-33 (major menaquinones MK-12 and MK-11) and Polar Lipid profile (major Polar Lipids diphosphatidyl glycerol, phosphatidyl glycerol and two unknown glycoLipids) were in accordance with those of Microbacterium paraoxydans strains CF36T, CF7 and CF40 which were analysed in this study as well. The results of biochemical/physiological characterisation, DNA-DNA hybridization, MALDI-TOF mass spectrometry of cell extracts and comparison of protein patterns after SDS-PAGE demonstrated that our isolate C57-33 (= DSM 15461) is a strain of the species Microbacterium paraoxydans. Based on new characteristics such as quinone system, Polar Lipid profile and physiological traits analysed for strain C57-33, the type strain of Microbacterium paraoxydans and some additional strains an emended description of the species Microbacterium paraoxydans is provided.
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identification of a bacterial strain isolated from the liver of a laboratory mouse as microbacterium paraoxydans and emended description of the species microbacterium paraoxydans laffineur et al 2003
Indian Journal of Microbiology, 2008Co-Authors: Sandra Buczolits, Maria Valens, Ramon Rossellomora, Peter Schumann, Hans-jürgen BusseAbstract:A rod-shaped, Gram-positive bacterial strain, designated C57-33, was isolated from the liver of the laboratory mouse strain C57Bl/6J and characterised by a polyphasic approach. 16S rRNA gene sequence similarity placed strain C57-33 in the genus Microbacterium with Microbacterium paraoxydans CF36T as the next relative (99.9% sequence similarity). Major fatty acids ai-C15:0, i-C16:0 and ai-C17:0 and peptidoglycan type B2β with ornithine as the diagnostic cell-wall diamino acid and glycolyl residues were in agreement with the description of Microbacterium paraoxydans. The quinone system of C57-33 (major menaquinones MK-12 and MK-11) and Polar Lipid profile (major Polar Lipids diphosphatidyl glycerol, phosphatidyl glycerol and two unknown glycoLipids) were in accordance with those of Microbacterium paraoxydans strains CF36T, CF7 and CF40 which were analysed in this study as well. The results of biochemical/physiological characterisation, DNA-DNA hybridization, MALDI-TOF mass spectrometry of cell extracts and comparison of protein patterns after SDS-PAGE demonstrated that our isolate C57-33 (= DSM 15461) is a strain of the species Microbacterium paraoxydans. Based on new characteristics such as quinone system, Polar Lipid profile and physiological traits analysed for strain C57-33, the type strain of Microbacterium paraoxydans and some additional strains an emended description of the species Microbacterium paraoxydans is provided.
Koen Dewettinck - One of the best experts on this subject based on the ideXlab platform.
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combined effects of milk fat globule membrane Polar Lipids and protein concentrate on the stability of oil in water emulsions
International Dairy Journal, 2016Co-Authors: Thi Thanh Que Phan, Davy Van De Walle, Paul Van Der Meeren, Koen DewettinckAbstract:Abstract Milk fat globule membrane (MFGM) material isolated from reconstituted buttermilk by microfiltration (i.e., whole MFGM) contains two major fractions, namely proteins (consisting mainly of MFGM-specific proteins and serum proteins) and Lipids (original membrane Polar Lipids and contaminating triglycerides from the globule core). In this study, MFGM proteins and Polar Lipid (PL) concentrate were separated from whole MFGM material using solvent extraction. The particle size distribution, stability, surface protein and Polar Lipid load of oil-in-water emulsions prepared with protein or PL concentrate, individually or in combination, at various concentrations were examined. At low emulsifier concentrations (
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thermocalcic aggregation of milk fat globule membrane fragments from acid buttermilk cheese whey
Journal of Dairy Science, 2007Co-Authors: Roeland Rombaut, Koen DewettinckAbstract:Fragments originating from the milk fat globule membrane (MFGM), which is rich in Polar Lipids and membrane-specific proteins, are gaining interest for their functional and nutritional properties. Acid buttermilk cheese whey was used as a source for MFGM purification, because its MFGM content is more than 5 times higher than that of standard rennet whey. Because Polar Lipids are the main constituent of the MFGM and only occur in membranous structures, the Polar Lipid content was taken as a parameter for the total MFGM fragment content. The process of thermocalcic aggregation was evaluated on its recovery of MFGM fragments in the pellet. This method, originally intended for whey clarification and defatting, is a combination of calcium addition, a pH increase, and a thermal treatment. The influence of pH (6.5 to 8), temperature (40 to 70°C), and calcium concentration (0.1 to 0.24 g/100 g) on the pellet mass and dry matter (DM) content and on recovery of protein and Polar Lipids (and thus indirectly on MFGM fragments) was investigated by means of a response surface Box-Behnken orthogonal design. Reduced quadratic models were fit to the experimental data and were found to be highly significant. No outliers were observed. The recovery of MFGM fragments was found to be highly dependent on the pH, and less dependent on temperature and calcium addition. Next to MFGM proteins, whey proteins were also found to be involved in the formation of aggregates. Optimal conditions were found at 55°C, pH 7.7, and 0.205 g of calcium/L of whey. Under these conditions, 91.0% of the whey Polar Lipids were recovered in a firm and compact pellet of only 7.86% of the original whey mass, with a Polar Lipid concentration of 8.34% on pellet DM. Washing with water and centrifugation of the pellet was successful because after one washing step, virtually all sugars were removed, whereas 75.9% of the whey Polar Lipids could still be recovered. As such, the Polar Lipid content of the washed pellet increased to 10.70% on a DM basis. However, a second washing step resulted in serious losses of MFGM material.
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filtration of milk fat globule membrane fragments from acid buttermilk cheese whey
Journal of Dairy Science, 2007Co-Authors: Roeland Rombaut, Veerle Dejonckheere, Koen DewettinckAbstract:The proteins and Polar Lipids present in milk fat globule membrane (MFGM) fragments are gaining attention for their technological and nutritional properties. These MFGM fragments are preferentially enriched in side streams of the dairy industry, like butter serum, buttermilk, and whey. The objective of this study was to recover MFGM fragments from whey by tangential filtration techniques. Acid buttermilk cheese whey was chosen as a source for purification by tangential membrane filtration because it is relatively rich in MFGM-fragments and because casein micelles are absent. Polyethersulfone and cellulose acetate membranes of different pore sizes were evaluated on Polar Lipid and MFGM-protein retention upon filtration at 40°C. All fractions were analyzed for dry matter, ash, Lipids, proteins, reducing sugars, Polar Lipid content by HPLC, and for the presence of MFGM proteins by sodium dodecyl sulfate-PAGE. A fouling coefficient was calculated. It was found that a thermocalcic aggregation whey pretreatment was very effective in the clarification of the whey, but resulted in low permeate fluxes and high retention of ash and whey proteins. By means of an experimental design, the influence of pH and temperature on the fouling and the retention of Polar Lipids (and thus MFGM fragments), proteins, and total Lipids upon microfiltration with 0.15 μM cellulose acetate membrane was investigated. All models were highly significant, and no outliers were observed. By increasing the pH from 4.6 to 7.5, Polar Lipid retention at 50°C increased from 64 to 98%, whereas fouling of the filtration membrane was minimized. A 3-step diafiltration of acid whey under these conditions resulted in a Polar Lipid concentration of 6.79 g/100 g of dry matter. As such, this study shows that tangential filtration techniques are suited for the purification of MFGM fragments.
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Analysis of Phospho- and SphingoLipids in Dairy Products by a New HPLC Method
Journal of Dairy Science, 2005Co-Authors: Roeland Rombaut, John Van Camp, Koen DewettinckAbstract:Dairy phospho- and sphingoLipids are gaining interest due to their nutritional and technological properties. A new HPLC method, using an evaporative laser light-scattering detector, was developed, which enabled excellent separation of glucosylceramide, lactosylceramide, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine in less than 21 min, including the regeneration of the column. No loss of column performance was observed after 1500 runs because an acid buffer was used. The output signal of the evaporative laser light scattering detector was highly dependent of the flow of the carrier gas and the temperature of the nebulizer, and was maximized by means of a response surface experimental design. Finally, raw milk, cream, butter, buttermilk, Cheddar whey, quarg, and Cheddar cheese were analyzed for their Polar Lipid content. The absolute values varied substantially (0.018 to 0.181 g/100 g of product). Significant differences were found in the relative content of each Polar Lipid class among the analyzed products.
David A. Sullivan - One of the best experts on this subject based on the ideXlab platform.
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complete androgen insensitivity syndrome effect on human meibomian gland secretions
Archives of Ophthalmology, 2002Co-Authors: Benjamin D Sullivan, James E. Evans, Kathleen L Krenzer, Jennifer M Cermak, Reza Dana, David A. SullivanAbstract:Objective To determine whether androgen receptors affect the fatty acid profiles of neutral and Polar Lipids in human meibomian gland secretions. Methods Meibomian gland secretion samples were obtained from both eyes of (1) women with complete androgen insensitivity syndrome, a condition characterized by dysfunctional androgen receptors, and (2) age-matched female and male controls. Samples were processed for high-performance liquid chromatography, mass spectrometry, or both and for analysis of the mass spectra of neutral and Polar Lipid fatty acid fragment ions by 3 different methods. Results Androgen receptor dysfunction is associated with significant alterations in the appearance of numerous molecular species in the neutral and Polar Lipid fractions of meibomian gland secretions. The ability to detect these differences, and to assess their nature and extent, was facilitated by the use of several analytic approaches. Sex-related differences exist in the expression of a variety of neutral and, especially, Polar fatty acid products in meibomian gland secretions. Conclusions Androgens exert a significant effect on neutral and Polar Lipids in human meibomian gland secretions, and these hormonal effects may be mediated through androgen receptors.
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correlations between nutrient intake and the Polar Lipid profiles of meibomian gland secretions in women with sjogren s syndrome
Advances in Experimental Medicine and Biology, 2002Co-Authors: Benjamin D Sullivan, James E. Evans, Jennifer M Cermak, R M Sullivan, Athena Papas, M R Dana, David A. SullivanAbstract:Very recently, we discovered that women with Sjogren’s syndrome exhibit one of two distinct profiles in the Polar Lipid fraction of their meibomian gland secretions (Fig. 1). The first pattern is characterized by a single predominant peak eluting early on in the total ion current during HPLC analysis. The second pattern is characterized by multiple peaks spread over time. To begin to explore the mechanism(s) underlying these differences, we sought to determine whether the nutrient intake, and particularly the Lipid consumption, of Sjogren’s syndrome patients may vary between those individuals expressing the “single” versus the “multiple” profile.
Benjamin D Sullivan - One of the best experts on this subject based on the ideXlab platform.
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complete androgen insensitivity syndrome effect on human meibomian gland secretions
Archives of Ophthalmology, 2002Co-Authors: Benjamin D Sullivan, James E. Evans, Kathleen L Krenzer, Jennifer M Cermak, Reza Dana, David A. SullivanAbstract:Objective To determine whether androgen receptors affect the fatty acid profiles of neutral and Polar Lipids in human meibomian gland secretions. Methods Meibomian gland secretion samples were obtained from both eyes of (1) women with complete androgen insensitivity syndrome, a condition characterized by dysfunctional androgen receptors, and (2) age-matched female and male controls. Samples were processed for high-performance liquid chromatography, mass spectrometry, or both and for analysis of the mass spectra of neutral and Polar Lipid fatty acid fragment ions by 3 different methods. Results Androgen receptor dysfunction is associated with significant alterations in the appearance of numerous molecular species in the neutral and Polar Lipid fractions of meibomian gland secretions. The ability to detect these differences, and to assess their nature and extent, was facilitated by the use of several analytic approaches. Sex-related differences exist in the expression of a variety of neutral and, especially, Polar fatty acid products in meibomian gland secretions. Conclusions Androgens exert a significant effect on neutral and Polar Lipids in human meibomian gland secretions, and these hormonal effects may be mediated through androgen receptors.
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correlations between nutrient intake and the Polar Lipid profiles of meibomian gland secretions in women with sjogren s syndrome
Advances in Experimental Medicine and Biology, 2002Co-Authors: Benjamin D Sullivan, James E. Evans, Jennifer M Cermak, R M Sullivan, Athena Papas, M R Dana, David A. SullivanAbstract:Very recently, we discovered that women with Sjogren’s syndrome exhibit one of two distinct profiles in the Polar Lipid fraction of their meibomian gland secretions (Fig. 1). The first pattern is characterized by a single predominant peak eluting early on in the total ion current during HPLC analysis. The second pattern is characterized by multiple peaks spread over time. To begin to explore the mechanism(s) underlying these differences, we sought to determine whether the nutrient intake, and particularly the Lipid consumption, of Sjogren’s syndrome patients may vary between those individuals expressing the “single” versus the “multiple” profile.
Roeland Rombaut - One of the best experts on this subject based on the ideXlab platform.
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thermocalcic aggregation of milk fat globule membrane fragments from acid buttermilk cheese whey
Journal of Dairy Science, 2007Co-Authors: Roeland Rombaut, Koen DewettinckAbstract:Fragments originating from the milk fat globule membrane (MFGM), which is rich in Polar Lipids and membrane-specific proteins, are gaining interest for their functional and nutritional properties. Acid buttermilk cheese whey was used as a source for MFGM purification, because its MFGM content is more than 5 times higher than that of standard rennet whey. Because Polar Lipids are the main constituent of the MFGM and only occur in membranous structures, the Polar Lipid content was taken as a parameter for the total MFGM fragment content. The process of thermocalcic aggregation was evaluated on its recovery of MFGM fragments in the pellet. This method, originally intended for whey clarification and defatting, is a combination of calcium addition, a pH increase, and a thermal treatment. The influence of pH (6.5 to 8), temperature (40 to 70°C), and calcium concentration (0.1 to 0.24 g/100 g) on the pellet mass and dry matter (DM) content and on recovery of protein and Polar Lipids (and thus indirectly on MFGM fragments) was investigated by means of a response surface Box-Behnken orthogonal design. Reduced quadratic models were fit to the experimental data and were found to be highly significant. No outliers were observed. The recovery of MFGM fragments was found to be highly dependent on the pH, and less dependent on temperature and calcium addition. Next to MFGM proteins, whey proteins were also found to be involved in the formation of aggregates. Optimal conditions were found at 55°C, pH 7.7, and 0.205 g of calcium/L of whey. Under these conditions, 91.0% of the whey Polar Lipids were recovered in a firm and compact pellet of only 7.86% of the original whey mass, with a Polar Lipid concentration of 8.34% on pellet DM. Washing with water and centrifugation of the pellet was successful because after one washing step, virtually all sugars were removed, whereas 75.9% of the whey Polar Lipids could still be recovered. As such, the Polar Lipid content of the washed pellet increased to 10.70% on a DM basis. However, a second washing step resulted in serious losses of MFGM material.
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filtration of milk fat globule membrane fragments from acid buttermilk cheese whey
Journal of Dairy Science, 2007Co-Authors: Roeland Rombaut, Veerle Dejonckheere, Koen DewettinckAbstract:The proteins and Polar Lipids present in milk fat globule membrane (MFGM) fragments are gaining attention for their technological and nutritional properties. These MFGM fragments are preferentially enriched in side streams of the dairy industry, like butter serum, buttermilk, and whey. The objective of this study was to recover MFGM fragments from whey by tangential filtration techniques. Acid buttermilk cheese whey was chosen as a source for purification by tangential membrane filtration because it is relatively rich in MFGM-fragments and because casein micelles are absent. Polyethersulfone and cellulose acetate membranes of different pore sizes were evaluated on Polar Lipid and MFGM-protein retention upon filtration at 40°C. All fractions were analyzed for dry matter, ash, Lipids, proteins, reducing sugars, Polar Lipid content by HPLC, and for the presence of MFGM proteins by sodium dodecyl sulfate-PAGE. A fouling coefficient was calculated. It was found that a thermocalcic aggregation whey pretreatment was very effective in the clarification of the whey, but resulted in low permeate fluxes and high retention of ash and whey proteins. By means of an experimental design, the influence of pH and temperature on the fouling and the retention of Polar Lipids (and thus MFGM fragments), proteins, and total Lipids upon microfiltration with 0.15 μM cellulose acetate membrane was investigated. All models were highly significant, and no outliers were observed. By increasing the pH from 4.6 to 7.5, Polar Lipid retention at 50°C increased from 64 to 98%, whereas fouling of the filtration membrane was minimized. A 3-step diafiltration of acid whey under these conditions resulted in a Polar Lipid concentration of 6.79 g/100 g of dry matter. As such, this study shows that tangential filtration techniques are suited for the purification of MFGM fragments.
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Analysis of Phospho- and SphingoLipids in Dairy Products by a New HPLC Method
Journal of Dairy Science, 2005Co-Authors: Roeland Rombaut, John Van Camp, Koen DewettinckAbstract:Dairy phospho- and sphingoLipids are gaining interest due to their nutritional and technological properties. A new HPLC method, using an evaporative laser light-scattering detector, was developed, which enabled excellent separation of glucosylceramide, lactosylceramide, phosphatidic acid, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine, phosphatidylcholine, sphingomyelin, and lysophosphatidylcholine in less than 21 min, including the regeneration of the column. No loss of column performance was observed after 1500 runs because an acid buffer was used. The output signal of the evaporative laser light scattering detector was highly dependent of the flow of the carrier gas and the temperature of the nebulizer, and was maximized by means of a response surface experimental design. Finally, raw milk, cream, butter, buttermilk, Cheddar whey, quarg, and Cheddar cheese were analyzed for their Polar Lipid content. The absolute values varied substantially (0.018 to 0.181 g/100 g of product). Significant differences were found in the relative content of each Polar Lipid class among the analyzed products.
