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Soonwook Yang - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory activity of Paenibacillus Polymyxa on the biofilm formation of Cronobacter spp. on stainless steel surfaces.
Journal of Food Science, 2013Co-Authors: Soonwook YangAbstract:The objective of this study was to control the survival or biofilm formation of Cronobacter spp. on stainless steel surfaces using Paenibacillus Polymyxa. The antibacterial activity of a cell-free culture supernatant (CFCS) of P. Polymyxa against Cronobacter spp. was found to vary with P. Polymyxa incubation time. Maximum activity occurred when P. Polymyxa was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to Cronobacter spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited Cronobacter biofilm formation. Additionally, stainless steel surfaces with a preformed P. Polymyxa biofilm were exposed to Cronobacter spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The Cronobacter population significantly decreased on this surface, regardless of inoculum level or carrier, when the P. Polymyxa biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that P. Polymyxa is able to use a promising candidate competitive-exclusion microorganism to control Cronobacter spp. Practical Application This study demonstrated inhibitory activity of Paenibacillus Polymyxa against biofilm formation of Cronobacter spp. on stainless steel surfaces and showed possibility of P. Polymyxa as a competitive-exclusion microorganism against Cronobacter spp. These findings will be useful when developing strategies to control Cronobacter spp. in food processing plants using P. Polymyxa.
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Inhibitory activity of Paenibacillus Polymyxa on the biofilm formation of Cronobacter spp. on stainless steel surfaces.
Journal of food science, 2013Co-Authors: Soonwook Yang, Seonhwa Kim, Jee Hoon Ryu, Hoikyung KimAbstract:The objective of this study was to control the survival or biofilm formation of Cronobacter spp. on stainless steel surfaces using Paenibacillus Polymyxa. The antibacterial activity of a cell-free culture supernatant (CFCS) of P. Polymyxa against Cronobacter spp. was found to vary with P. Polymyxa incubation time. Maximum activity occurred when P. Polymyxa was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to Cronobacter spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited Cronobacter biofilm formation. Additionally, stainless steel surfaces with a preformed P. Polymyxa biofilm were exposed to Cronobacter spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The Cronobacter population significantly decreased on this surface, regardless of inoculum level or carrier, when the P. Polymyxa biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that P. Polymyxa is able to use a promising candidate competitive-exclusion microorganism to control Cronobacter spp.
Hoikyung Kim - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory activity of Paenibacillus Polymyxa on the biofilm formation of Cronobacter spp. on stainless steel surfaces.
Journal of food science, 2013Co-Authors: Soonwook Yang, Seonhwa Kim, Jee Hoon Ryu, Hoikyung KimAbstract:The objective of this study was to control the survival or biofilm formation of Cronobacter spp. on stainless steel surfaces using Paenibacillus Polymyxa. The antibacterial activity of a cell-free culture supernatant (CFCS) of P. Polymyxa against Cronobacter spp. was found to vary with P. Polymyxa incubation time. Maximum activity occurred when P. Polymyxa was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to Cronobacter spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited Cronobacter biofilm formation. Additionally, stainless steel surfaces with a preformed P. Polymyxa biofilm were exposed to Cronobacter spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The Cronobacter population significantly decreased on this surface, regardless of inoculum level or carrier, when the P. Polymyxa biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that P. Polymyxa is able to use a promising candidate competitive-exclusion microorganism to control Cronobacter spp.
Sanfeng Chen - One of the best experts on this subject based on the ideXlab platform.
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Identification of Genes Involved in Fe–S Cluster Biosynthesis of Nitrogenase in Paenibacillus Polymyxa WLY78
International Journal of Molecular Sciences, 2021Co-Authors: Sanfeng ChenAbstract:NifS and NifU (encoded by nifS and nifU) are generally dedicated to biogenesis of the nitrogenase Fe–S cluster in diazotrophs. However, nifS and nifU are not found in N2-fixing Paenibacillus strains, and the mechanisms involved in Fe–S cluster biosynthesis of nitrogenase is not clear. Here, we found that the genome of Paenibacillus Polymyxa WLY78 contains the complete sufCDSUB operon, a partial sufC2D2B2 operon, a nifS-like gene, two nifU-like genes (nfuA-like and yutI), and two iscS genes. Deletion and complementation studies showed that the sufC, sufD, and sufB genes of the sufCDSUB operon, and nifS-like and yutI genes were involved in the Fe–S cluster biosynthesis of nitrogenase. Heterologous complementation studies demonstrated that the nifS-like gene of P. Polymyxa WLY78 is interchangeable with Klebsiella oxytoca nifS, but P. Polymyxa WLY78 SufCDB cannot be functionally replaced by K. oxytoca NifU. In addition, K. oxytoca nifU and Escherichia coli nfuA are able to complement the P. Polymyxa WLY78 yutI mutant. Our findings thus indicate that the NifS-like and SufCDB proteins are the specific sulfur donor and the molecular scaffold, respectively, for the Fe–S cluster formation of nitrogenase in P. Polymyxa WLY78. YutI can be an Fe–S cluster carrier involved in nitrogenase maturation in P. Polymyxa WLY78.
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Colonization of Wheat, Maize and Cucumber by Paenibacillus Polymyxa WLY78.
PloS one, 2017Co-Authors: Tianyi Hao, Sanfeng ChenAbstract:Paenibacillus Polymyxa WLY78 is a nitrogen fixer and it can be potentially applied to biofertilizer in agriculture. In this study, P. Polymyxa WLY78 is labelled with gfp gene. The GFP-labelled P. Polymyxa WLY78 is used to inoculate wheat, maize and cucumber seedlings grown in the gnotobiotic system and in soil, respectively. Observation by confocal laser scanning microscope reveals that the GFP-labeled bacterial cells are mainly located on the root surface and epidermis of wheat, and only a few cells are present within cortical cells. In maize and cucumber seedlings, bacterial cells were colonized in epidermal and cortical cells, intercellular spaces and vascular system of root, stem and leaf tissue interiors besides on root surfaces. Higher densities of the bacterial cells in roots, stems and leaves indicated that P. Polymyxa WLY78 cells could migrate from roots to stems and leaves of maize and cucumber. This study will provide insight into interaction between P. Polymyxa WLY78 and host cells.
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Cloning and sequence analysis ofglnB-like gene from nitrogen-fixingPaenibacillus Polymyxa G2
Annals of Microbiology, 2006Co-Authors: Yanqin Ding, Sanfeng ChenAbstract:A 240 bpglnB-like gene fragment was PCR amplified fromPaenibacillus Polymyxa G2 using universal degenerate primers. These degenerate primers, based on all knownglnB-like genes including thenrgB ofBacillus subtilis at the time of design, were chosen from highly conserved amino acid sequences. The GlnB-like sequence ofP. Polymyxa G2 had relatively high identities (more than 70%) to other bacteria GlnB, e.g.Escherichia coli (78%) andKlebsiella pneuomoniae (79%). However, the identity of theP. Polymyxa GlnB-like sequence to a GlnK homologue (NrgB) of B.subtilis was low (46%). This is the first report of the sequence of theglnB-like gene from the genusPaenibacillus. Knowledge of theglnB-like gene sequence ofP. Polymyxa will make us study deeply the function ofglnB in the genusPaenibacillus.
Jee Hoon Ryu - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory activity of Paenibacillus Polymyxa on the biofilm formation of Cronobacter spp. on stainless steel surfaces.
Journal of food science, 2013Co-Authors: Soonwook Yang, Seonhwa Kim, Jee Hoon Ryu, Hoikyung KimAbstract:The objective of this study was to control the survival or biofilm formation of Cronobacter spp. on stainless steel surfaces using Paenibacillus Polymyxa. The antibacterial activity of a cell-free culture supernatant (CFCS) of P. Polymyxa against Cronobacter spp. was found to vary with P. Polymyxa incubation time. Maximum activity occurred when P. Polymyxa was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to Cronobacter spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited Cronobacter biofilm formation. Additionally, stainless steel surfaces with a preformed P. Polymyxa biofilm were exposed to Cronobacter spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The Cronobacter population significantly decreased on this surface, regardless of inoculum level or carrier, when the P. Polymyxa biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that P. Polymyxa is able to use a promising candidate competitive-exclusion microorganism to control Cronobacter spp.
Seonhwa Kim - One of the best experts on this subject based on the ideXlab platform.
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Inhibitory activity of Paenibacillus Polymyxa on the biofilm formation of Cronobacter spp. on stainless steel surfaces.
Journal of food science, 2013Co-Authors: Soonwook Yang, Seonhwa Kim, Jee Hoon Ryu, Hoikyung KimAbstract:The objective of this study was to control the survival or biofilm formation of Cronobacter spp. on stainless steel surfaces using Paenibacillus Polymyxa. The antibacterial activity of a cell-free culture supernatant (CFCS) of P. Polymyxa against Cronobacter spp. was found to vary with P. Polymyxa incubation time. Maximum activity occurred when P. Polymyxa was incubated at 25 or 30 °C for 96 h. When the CFCS was introduced to Cronobacter spp. adhered to stainless steel strips at 25 °C for up to 72 h, the CFCS successfully inhibited Cronobacter biofilm formation. Additionally, stainless steel surfaces with a preformed P. Polymyxa biofilm were exposed to Cronobacter spp. suspensions in PBS or 0.1% peptone water at 3, 5, or 7 log CFU/mL to facilitate its attachment. The Cronobacter population significantly decreased on this surface, regardless of inoculum level or carrier, when the P. Polymyxa biofilm was present. However, the microbial population decreased within 6 h and remained unchanged thereafter when the surface was immersed in an inoculum suspended in 0.1% peptone water at 5 or 7 log CFU/mL. These results indicate that P. Polymyxa is able to use a promising candidate competitive-exclusion microorganism to control Cronobacter spp.
