The Experts below are selected from a list of 246 Experts worldwide ranked by ideXlab platform

Shangjin Cui - One of the best experts on this subject based on the ideXlab platform.

  • Genome Sequence of Chinese Porcine Parvovirus Strain PPV2010
    Journal of virology, 2012
    Co-Authors: Jin Cui, Xin Wang, Yudong Ren, Shangjin Cui, Xiaofeng Ren
    Abstract:

    Porcine Parvovirus (PPV) isolate PPV2010 has recently emerged in China. Herein, we analyze the complete genome sequence of PPV2010. Our results indicate that the genome of PPV2010 bears mixed characteristics of virulent PPV and vaccine strains. Importantly, PPV2010 has the potential to be a naturally attenuated candidate vaccine strain.

  • The epitope of the VP1 protein of Porcine Parvovirus.
    Virology journal, 2010
    Co-Authors: Hong-ling Xie, Shangjin Cui, Zhao Wang, Chao-fan Zhang, Yu-dong Cui
    Abstract:

    Porcine Parvovirus (PPV) is the major causative agent in a syndrome of reproductive failure in swine. Much has been learned about the structure and function of PPV in recent years, but nothing is known about the epitopes of the structural protein VP1, which is an important antigen of PPV. In this study, the monoclonal antibody C4 against VP1 of PPV was prepared and was used to biopan a 12-mer phage peptide library three times. The selected phage clones were identified by ELISA and then sequencing. The amino acid sequences detected by phage display were analyzed, and a mimic immuno-dominant epitope was identified. The epitope of VP1 is located in the N-terminal and contains the role amino acid sequence R-K-R. Immunization of mice indicated that the phage-displayed peptide induces antibodies against PPV. This study shows that peptide mimotopes have potential as alternatives to the complex antigens currently used for diagnosis of PPV infection or for development of vaccines.

  • detection of Porcine Parvovirus by loop mediated isothermal amplification
    Journal of Virological Methods, 2009
    Co-Authors: Changmu Chen, Shangjin Cui
    Abstract:

    Abstract Loop-mediated isothermal amplification is a novel method for rapid amplification of DNA. It has been adopted widely for the detection of virus because of its simplicity, rapidity, and specificity. A loop-mediated isothermal amplification assay was developed for the detection of Porcine Parvovirus. Four primers specific for six regions of PPV non-structural protein 1 gene were designed with an online software. After amplifying at a constant temperature of 59–65 °C by Bst enzyme, a clear result was visible after 2.5% agarose gel electrophoresis. The sensitivity and specificity of this assay were evaluated by comparison with the polymerase chain reaction. The detection limit of the assay was shown to be equivalent to 5 PPV copies/reaction. Due to its specificity and simplicity, the assay should be a useful diagnostic tool for epidemiologic studies of PPV.

Peter Tijssen - One of the best experts on this subject based on the ideXlab platform.

  • Biology of Porcine Parvovirus (Ungulate Parvovirus 1)
    Viruses, 2017
    Co-Authors: István Mészáros, Peter Tijssen, Ferenc Olasz, Attila Cságola, Zoltan Zadori
    Abstract:

    Porcine Parvovirus (PPV) is among the most important infectious agents causing infertility in pigs. Until recently, it was thought that the virus had low genetic variance, and that prevention of its harmful effect on pig fertility could be well-controlled by vaccination. However, at the beginning of the third millennium, field observations raised concerns about the effectiveness of the available vaccines against newly emerging strains. Subsequent investigations radically changed our view on the evolution and immunology of PPV, revealing that the virus is much more diverse than it was earlier anticipated, and that some of the "new" highly virulent isolates cannot be neutralized effectively by antisera raised against "old" PPV vaccine strains. These findings revitalized PPV research that led to significant advancements in the understanding of early and late viral processes during PPV infection. Our review summarizes the recent results of PPV research and aims to give a comprehensive update on the present understanding of PPV biology.

  • Phylogeny and evolution of Porcine Parvovirus.
    Virus Research, 2013
    Co-Authors: Xiaofeng Ren, Jin Cui, Ye Tao, Siqingaowa Suo, Yingying Cong, Peter Tijssen
    Abstract:

    Porcine Parvovirus (PPV), a member of the genus Parvovirus, family Parvoviridae, is a significant causative agent in Porcine reproductive failure, causing serious economic losses in the swine industry. Previous phylogenetic studies based on the NS1 or VP2 genes indicated that current PPV strains diverged 30 years ago and that VP2 was under neutral or positive selection. Our analysis of NS1, VP2 and complete ORFs indicated that the most recent common ancestor of PPV strains existed about 250 years ago and that the 127-nt repeat in the 3'NTR was present in viruses of some subclades that evolved about 80 years ago. Nucleotide substitution rates of NS1 and VP2 genes were 3.03 × 10(-5) and 1.07 × 10(-4), respectively. Both the NS1 and VP2 proteins were under purifying selection and recombination did not contribute to the genetic diversity of PPV. As expected, surface amino acids are hydrophilic and make up the majority of mutations in the VP2 protein; residues in VP2 interfaces were substituted gradually, often in conjunction with complementary substitutions in the neighboring VP2.

  • The structure of Porcine Parvovirus: comparison with related viruses
    Journal of molecular biology, 2002
    Co-Authors: Alan A. Simpson, Peter Tijssen, Zoltan Zadori, Benoı̂t Hébert, Gail M. Sullivan, Colin R. Parrish, Michael G. Rossmann
    Abstract:

    The structure of baculovirus-expressed Porcine Parvovirus (PPV) capsids was solved using X-ray crystallography and was found to be similar to the related canine Parvovirus (CPV) and minute virus of mice (MVM). The PPV capsid protein has 57 % and 49 % amino acid sequence identity with CPV and MVM, respectively, but the degree of conservation of surface-exposed residues is lower than average. Consequently, most of the structural differences are on the surface and are the probable cause of the known variability in antigenicity and host range. The NADL-2 and Kresse strains of PPV have distinct tissue tropisms and pathogenicity, which are mediated by one or more of the amino acid residues 381, 386, and 436. These residues are on or near the surface of the virus capsid, where they are likely to be associated with virus-cell interactions.

T W Molitor - One of the best experts on this subject based on the ideXlab platform.

  • A monoclonal antibody which recognizes cell surface antigen and inhibits Porcine Parvovirus replication
    Archives of Virology, 1992
    Co-Authors: M. J. Harding, T W Molitor
    Abstract:

    Monoclonal antibody technologies were applied to the study of early events in Porcine Parvovirus (PPV) infections in vitro. Balb/c mice were immunized with whole swine testicle cells and hybridomas were produced following fusion with myeloma cells. Resultant clones were screened firstly in an ELISA system, to detect monoclonal antibody recognition of swine testicle cells, and secondly, in a fluorescent antibody test to detect monoclonal antibody which inhibited production of PPV antigen. One clone, 1H11, which satisfied these screening requirements, recognized proteins present in cell lines both permissive and non-permissive for Porcine Parvovirus replication and inhibited the production of virus progeny of several PPV isolates. A linear staining pattern of cross-linked plasma membranes, indicative of monoclonal antibody binding at the cell membrane, was demonstrated by indirect immunofluorescence assays. In immunoblotting experiments, 1H11 recognized a polypeptide of approximately 40 kDa in size, present in both permissive and non-permissive cell lines.

Chong Wang - One of the best experts on this subject based on the ideXlab platform.

  • In vitro antiviral activity of germacrone against Porcine Parvovirus.
    Archives of virology, 2015
    Co-Authors: Ye Chen, Yunxia Dong, Yiren Jiao, Lianjie Hou, Yuzhen Shi, Pei Zhou, Shi Zhongyuan, Chong Wang
    Abstract:

    Porcine Parvovirus (PPV) infections can lead to significant losses to the swine industry by causing reproductive failure in pigs. Germacrone has been reported to efficiently suppress the replication of influenza virus. In this report, the antiviral activity of germacrone on PPV in swine testis (ST) cells was investigated. Here, we show for the first time that germacrone protects cells from PPV infection and suppresses the synthesis of viral mRNA and protein. Furthermore, we show that germacrone inhibits PPV replication at an early stage in a dose-dependent manner. These findings suggest that germacrone is a potential candidate for anti-PPV therapy.

Cui Bao-an - One of the best experts on this subject based on the ideXlab platform.

  • Development of SYBR Green I real-time PCR assay for detection of Porcine Parvovirus.
    2009
    Co-Authors: Li Mingfeng, Wang Xue-bin, Wei Zhanyong, Zhang Hongying, Wang Yabin, Cui Bao-an
    Abstract:

    A 194 bp region of the Porcine Parvovirus VP2 gene was amplified by PCR and cloned into pGEM T vector,named as pGEM-VP2.Serial dilutions of plasmid pGEM-VP2 were used as standard templates for PCR to quantify the virus genomic copy number.We developed a SYBR Green Ⅰreal-time PCR to detect Porcine Parvovirus.Sensitivity analysis showed that the developed SYBR Green I real-time PCR could detect 1.0×102 template/μL.The specificity assay exhibited that negative control and the other Porcine pathogens,such as PRRSV,PCV,JEV,PRV,HCV and SIV could not be detected by this PCR.It was showed that 10 positive results could be observed by the real-time PCR for 10 suspicious positive samples,but 7 positive results by normal PCR.The results suggested that as a result of the good sensitivity and specificity of the assay with a rapid and simple procedure,the real-time PCR method would be useful for the clinical diagnosis of Porcine Parvovirus infection.

  • Immune Efficiency of inactivated Vaccine of Porcine Parvovirus and Pseudorabies Virus
    Progress in Veterinary Medicine, 2003
    Co-Authors: Yang Mingfan, Cui Bao-an, Zhang Sumei, Wang Xue-bin, Xu Duanhong, Yang Jiangyuan
    Abstract:

    The immune efficiency test of inactivated vaccine of Porcine Parvovirus and Pseudorabies virus showed that PPV HI titers were over 1∶320(1∶320~1∶640 in)after sows were immunized and PRV Micro-neutralization indexs of antibody were over 1400.The result also proved that inactivated vaccine could protect sows from PPV and PRV infection.