The Experts below are selected from a list of 1902 Experts worldwide ranked by ideXlab platform
Koki Taniguchi - One of the best experts on this subject based on the ideXlab platform.
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Porcine Rotavirus closely related to novel group of human Rotaviruses
Emerging Infectious Diseases, 2011Co-Authors: Mitsutaka Wakuda, Satoshi Komoto, Junichi Ishii, Jun Sasaki, Takeshi Sanekata, Koki TaniguchiAbstract:We determined nucleotide sequences and inferred amino acid sequences of viral protein (VP) 4, VP6, VP7, and nonstructural protein 4 genes of a Porcine Rotavirus strain (SKA-1) from Japan. The strain was closely related to a novel group of human Rotavirus strains (B219 and J19).
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New P serotype of group A human Rotavirus closely related to that of a Porcine Rotavirus.
Journal of Medical Virology, 2000Co-Authors: Jun-ichi Okada, Koki Taniguchi, Tomoko Urasawa, N. Kobayashi, Ayako Hasegawa, Keiji Mise, Shozo UrasawaAbstract:The VP7 and VP4 genes of two human group A Rotavirus strains Mc323 and Mc345 with unique serologic and genomic properties, and isolated in Chiang Mai, Thailand, in 1989 [Urasawa et al. (1992) Journal of Infectious Diseases 166:227-234] were further characterized. The nucleotide and deduced amino acid sequences of the VP7 genes allowed the classification of both strains as serotype G9. The VP4 genes of both strains are 2,359 nucleotides in length and encode a protein of 775 amino acids like in most human Rotaviruses. A comparison of the VP4 amino acid sequence of strain Mc323 with those of strain Mc345 and 24 human and animal Rotaviruses representing 20 distinct VP4 genotypes reported to date showed that VP4 of Mc323 and Mc345 belong to genotype 19 previously reported for Porcine Rotavirus [Burke et al. (1994) Journal of General Virology 75:2205-2212]. To investigate the serological type (P serotype) of these VP4s, six reassortant viruses each containing a distinct VP4 gene characteristic of human Rotaviruses and the VP7 gene of Porcine Rotavirus strain Gottfried (G4) were prepared, and antisera to these reassortants produced in rabbits. In neutralization tests, the P serotype of Mc323 was clearly differentiated from the five major P serotypes reported previously for human Rotaviruses, suggesting that Mc323 and Mc345 represent a new human Rotavirus P serotype tentatively called P11.
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Similarity of the outer capsid protein VP4 of the Gottfried strain of Porcine Rotavirus to that of asymptomatic human Rotavirus strains.
Journal of Virology, 1990Co-Authors: Mario Gorziglia, Kazuo Nishikawa, Y Hoshino, Koki TaniguchiAbstract:Abstract Genomic segment 4 of the Porcine Gottfried strain (serotype 4) of Porcine Rotavirus, which encodes the outer capsid protein VP4, was sequences, and its deduced amino acid sequence was analyzed. Amino acid homology of the Porcine Rotavirus VP4 to the corresponding protein of asymptomatic or symptomatic human Rotaviruses representing serotypes 1 to 4 ranged from 87.1 to 88.1% for asymptomatic strains and from 77.5 to 77.8% for symptomatic strains. Amino acid homology of the Gottfried strain to simian rhesus Rotavirus, simian SA11 virus, bovine Nebraska calf diarrhea virus, and Porcine OSU strains ranged from 71.5 to 74.3%. Antigenic similarities of VP4 epitopes between the Gottfried strain and human Rotaviruses were detected by a plaque reduction neutralization test with hyperimmune antisera produced against the Gottfried strain or a Gottfried (10 genes) x human DS-1 Rotavirus (VP7 gene) reassortant which exhibited serotype 2 neutralization specificity. In addition, a panel of six anti-VP4 monoclonal antibodies capable of neutralizing human Rotaviruses belonging to serotype 1, 3, or 4 was able to neutralize the Gottfried strain. These observations suggest that the VP4 outer capsid protein of the Gottfried Rotavirus is more closely related to human Rotaviruses than to animal Rotaviruses.
Chen Shu-hong - One of the best experts on this subject based on the ideXlab platform.
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Isolation and characterization of Porcine Rotavirus
Chinese Journal of Preventive Veterinary Medicine, 2004Co-Authors: Chen Shu-hongAbstract:Porcine Rotavirus was successfully isolated from diarrheal piglet fecal samples from Jilin Province. MA104 cells were used for the replication and isolation of the virus. After four generations , the strain could produce obvious cytopathic effects (CPE). Typical Rotavirus particles were seen with direct electron microscope. Its RNA electropherotype was 4:2:3:2 which accords with the electropherotype of group A Rotavirus. Its characteristics of hemagglutination and plague were studied tentatively. The strain of Rotavirus was designated JL94.
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Cloning of VP6 gene from Porcine Rotavirus JL94 isolate and homologous comparison with reference strains
Chinese Journal of Veterinary Medicine, 2003Co-Authors: Chen Shu-hongAbstract:The Porcine Rotavirus JL94 isolate was propagated in MA104 cells and its dsRNA was extracted.Its cDNA segments of complete gene 6 were obtaied from RT-PCR and cloned into pMD18-Tvector and sequenced.The results of sequencing showed that JL94 complete gene 6 cDNA is 1356bp.The sequence was analyzed homologously in comparison with the VP6 nucleotide sequences of two reference Porcine Rotavirus OSU(subgroup Ⅰ)and Gottfried(subgroup Ⅱ).Nucleotide sequence comparison indicated that JL94 shared 86.85% and 82.41% identities with OSU and Gottfried respectively.The deduced animo acid sequences were 97.48% and 93.20% correspondingly.The result showed that JL94 isolate is more similar to OSU in gene type.
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Cloning and sequence analysis of gene encoding outer capsid protein VP7 of Porcine Rotavirus(RV) JL94 strain isolated in China
Chinese Journal of Veterinary Medicine, 2003Co-Authors: Chen Shu-hongAbstract:The native JL94 strain of Porcine Rotavirus was propagated and harvested on MA104 cell. Using a pair of primer designed according to the published cDNA sequence of RV's VP7 gene , the full length gene encoding outer capsid protein VP7 was amplified from JL94 strain by reverse transcription-polymerase chain reaction (RT-PCR). The cDNA of VP7gene was inserted into pMD18-T vector and was identified by sequencing, single and double digestion using HindⅢ, HindⅢ and BamHⅠ.The result of its homology comparison with C134/VP7, OSU/VP7, ICB2185/VP7 and YM/VP7 is 87%, 99%, 74% and 83% respectively.
Li Yi-jing - One of the best experts on this subject based on the ideXlab platform.
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Development of colloidal gold immunochromatographic test strip for detection of Porcine Rotavirus Group A
Chinese Veterinary Science, 2008Co-Authors: Li Yi-jingAbstract:To develop a rapid and simple assay for detection of Porcine Rotavirus(PRV),colloidal gold particles labeled with purified monoclonal antibody against PRV VP6 protein were prepared by reduction of sodium citrate.The purified polyclonal antibodies against PRV VP6 protein and goat anti-mouse IgG were solidified to nitrocellulose filter to prepare the colloidal gold immunochromatographic strip.MA104 cell cultures infected with PRV and non-infected were detected by the strips,respectively.The results showed that there was a red reaction strip at the test line only when detecting the infected cells,and the red reaction strips all appeared at the control line whatever infected.The test strips detecting virus culture(TCID50:10-6.25/0.1 mL) had a dilution 1∶32,which did not react with transmissible gastroenteritis virus of swine(TGEV) and Porcine epidemic diarrhea virus(PEDV),and the results of different batches of test strips were the same.These results revealed that the test strip has good sensitivity,reproducibility,and specificity.
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Analysis of Capsid Protein Gene of Porcine Rotavirus JL94 Isolate in China
Progress in Biotechnology, 2005Co-Authors: Li Yi-jingAbstract:Three pairs of primers designed according to the published conservative sequence of Porcine Rotavirus vp4 gene 、 vp6 gene and vp7 gene were used in cloning and sequencing analysis, respectively. The results of PRV/VP4 homology comparison with CRW-8 strain、 OSU strain 、 Gottfried strain、 4F strain and 4S strain were 97.16% , 95% , 71.88% , 73.45% and 70.88% respectively. The results of PRV/VP6 homology comparison with CRW-8 strain、OSU strain、 Gottfried strain、4F strain and 4S strain were 96.98% 、97.48% 、93 .20% 、97.48% and 93% respectively. The results of PRV/VP7 homology comparison with CRW-8 strain、OSU strain、Gottfried strain、4F strain and 4S strain were 98% 、99.90% 、75.40% 、84.66% and 80% respectively. The homology between JL94 and CRW-8 strain, OSU strain were higher. The vp4 gene and vp7 gene is highly conserved between isotypes, and different significantly between variant isotypes. The vp6 gene is not obviously different between isotypes and variant isotypes. PRV/JL94 belongs to I subgroup of A group and G5P7 serotype. Researching the feature of capsid protein gene form Porcine Rotavirus JL94 strain in China is to lay a foundation for developing concentrated antivirotic vaccine.
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Cloning of VP4 gene from Porcine Rotavirus JL94 isolate and homologous comparison with reference strains
Chinese Journal of Preventive Veterinary Medicine, 2004Co-Authors: Li Yi-jingAbstract:A pair of primers according to the published cDNA sequence of Porcine Rotavirus VP4 gene were designed, which were used in cloning and sequence analysis of VP4 gene from Porcine Rotavirus JL94 isolated in China. The full length gene encoding outer capid protein VP4 was amplified from the native JL94 isolate of Porcine Rotavirus by reverse transcription polymerase chain reaction(RTPCR). The cDNA of VP4 gene was inserted into pMD-18T vector and identified by sequencing. The result of its homology comparison with CRW-8 strain and BEN-307 strain is 96.98 % and 98.05 % respectively.
S González - One of the best experts on this subject based on the ideXlab platform.
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VP6 from Porcine Rotavirus strain CN86: amino acid sequence divergence with conservation of subgroup II specificity
Journal of General Virology, 1995Co-Authors: M. Alejandra Tortorici, L. Tomasini, J. Affranchino, S GonzálezAbstract:A Porcine Rotavirus strain, CN86, originally isolated from Rotavirus-infected piglets in Argentina, has been shown to possess unique characteristics. It was the first animal strain described to be antigenically related to human serotype G1 and the standard counterpart of another Porcine strain showing rearrangement of genome segment 11. Owing to these features, molecular characterization of this virus seemed relevant. The gene encoding the major inner capsid protein, VP6, was cloned and its nucleotide sequence was determined. Comparative analysis of the deduced amino acid sequence of CN86 VP6 with those representing the four different subgroups showed that it is more closely related to subgroup II human Wa and Porcine Gottfried strains, albeit to a lesser extent than they are to each other. Despite exhibiting sequence divergence, CN86 VP6 has 12 out of the 14 residues expected to be conserved in strains bearing subgroup II specificity. Interestingly, CN86 VP6 shows a high degree of homology with VP6 of Porcine strain YM Rotavirus which, although being closely related to subgroup II strains, has been serologically characterized as subgroup I. Subgroup II reactivity of CN86 strain, predicted by sequence analysis, was confirmed by ELISA with subgroup-specific monoclonal antibodies. Taken together, our results provide evidence for the existence of a human-pig lineage for Rotavirus gene 6.
Mitsutaka Wakuda - One of the best experts on this subject based on the ideXlab platform.
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Porcine Rotavirus closely related to novel group of human Rotaviruses
Emerging Infectious Diseases, 2011Co-Authors: Mitsutaka Wakuda, Satoshi Komoto, Junichi Ishii, Jun Sasaki, Takeshi Sanekata, Koki TaniguchiAbstract:We determined nucleotide sequences and inferred amino acid sequences of viral protein (VP) 4, VP6, VP7, and nonstructural protein 4 genes of a Porcine Rotavirus strain (SKA-1) from Japan. The strain was closely related to a novel group of human Rotavirus strains (B219 and J19).
