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Graham J. Belsham - One of the best experts on this subject based on the ideXlab platform.

  • Comparison of complete nucleotide and deduced amino acid sequences between the Korean Porcine sapelovirus (PSV) strains and other known picornavirus strains.
    2014
    Co-Authors: Kyu-yeol Son, Graham J. Belsham, Deok-song Kim, Joseph Kwon, Jong-soon Choi, Mun-il Kang, Kyoung-oh Cho
    Abstract:

    1GenBank accession numbers of strains used are in Table S2.2The full-length nucleotide sequence identities among PSVs and other picornaviruses.3The full-length deduced amino acid sequence identities among PSVs and other picornaviruses.4ASV: avian sapelovirus.5SSV: simian sapelovirus.6PTV-1: Porcine Teschovirus serotype 1.7PEV-9: Porcine enterovirus serotype 9.8EMCV: encephalomyocarditis virus.9FMDV: foot-and-mouth disease virus type O.10ERBV-1: Equine rhinitis B virus serotype 1.11PV-1: Poliovirus serotype 1(Human enterovirus C serotype).12HAV: Hepatitis A virus.13AiV: Aichi virus.14HPeV-1: Human parechovirus serotype 1.15DHVA: Duck hepatitis A virus.16SVV: Seneca Valley virus.17AEV: Avian encephalomyelitis virus.Comparison of complete nucleotide and deduced amino acid sequences between the Korean Porcine sapelovirus (PSV) strains and other known picornavirus strains.

  • Divergent picornavirus IRES elements.
    Virus Research, 2008
    Co-Authors: Graham J. Belsham
    Abstract:

    Internal ribosome entry site (IRES) elements were first identified about 20 years ago within the 5' untranslated region of picornavirus RNAs. They direct a cap-independent mechanism of translation initiation on the viral RNA. Within the picornavirus family it is now known that there are four classes of IRES element which vary in size (450-270 nt), they also have different, complex, secondary structures and distinct requirements for cellular proteins to allow them to function. This review describes the features of each class of picornavirus IRES element but focuses on the characteristics of the most recently described group, initially identified within the Porcine Teschovirus-1 RNA, which has strong similarities to the IRES elements from within the genomes of hepatitis C virus and the pestiviruses which are members of the flavivirus family. The selection of the initiation codon by these distinct IRES elements is also discussed.

  • Hepatitis C virus-related internal ribosome entry sites are found in multiple genera of the family Picornaviridae.
    Journal of General Virology, 2006
    Co-Authors: Louisa S. Chard, Marie-eve Bordeleau, Jerry Pelletier, Junichi Tanaka, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) elements from Porcine enterovirus 8 and simian virus 2, two members of a proposed new genus within the family Picornaviridae, were characterized. These IRES elements, in common with the Porcine Teschovirus 1 IRES, were found to be related functionally and structurally to the IRES element from Hepatitis C virus, a member of the family Flaviviridae. Partial secondary structure predictions were derived and functional assays demonstrated that these IRES elements continued to be active when eIF4G was cleaved and when the activity of eIF4A was blocked.

  • functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus Porcine Teschovirus 1 talfan
    Journal of Virology, 2006
    Co-Authors: Louisa S. Chard, Y Kaku, Barbara Jones, Arabinda Nayak, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) of Porcine Teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

  • functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus Porcine Teschovirus 1 talfan
    Journal of Virology, 2006
    Co-Authors: Louisa S. Chard, Barbara Jones, Arabinda Nayak, Yoshihiro Kaku, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) of Porcine Teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

Y Kaku - One of the best experts on this subject based on the ideXlab platform.

  • pathological changes in pigs experimentally infected with Porcine Teschovirus
    Journal of Comparative Pathology, 2009
    Co-Authors: M. Yamada, Y Kaku, M Yoshii, R Kozakura, Hiroshi Tsunemitsu, Y Yamamoto, K. Nakamura, A Miyazaki, M. Narita
    Abstract:

    Summary Nonsuppurative encephalomyelitis with neurological signs expressed as flaccid paralysis of the hindlimbs was experimentally induced in three-week-old piglets by a single intravenous injection of the Toyama 2002 strain of Porcine Teschovirus (PTV) isolated from field pigs in Japan. Lesions characterized by perivascular cuffing of mononuclear cells, focal gliosis, neuronal necrosis and neuronophagia were observed, mainly in the ventral horn of the spinal cord. Nonsuppurative ganglionitis of the spinal ganglion and neuritis of the spinal root were also detected. PTV antigens were detected immunohistochemically and the distribution of these antigens corresponded closely with the distribution of brain lesions. PTV antigens were observed in the ganglion cells before the appearance of the inflammatory changes 3 days post-inoculation (dpi) and were present in the dorsal root and spinal cord on 9 dpi. No lesions of the central nervous system were induced in pigs by oral or intranasal inoculation of this strain of PTV.

  • immunohistochemical distribution of viral antigens in pigs naturally infected with Porcine Teschovirus
    Journal of Veterinary Medical Science, 2008
    Co-Authors: Manabu Yamada, Y Kaku, M Yoshii, R Kozakura, Hiroshi Tsunemitsu, Y Yamamoto, K. Nakamura, A Miyazaki, M. Narita
    Abstract:

    A distribution of Porcine Teschovirus (PTV) antigens in pigs naturally infected with PTV is presented using the method of immunohistochemical examination. In the nervous system, PTV antigens were found in the cytoplasm of neuronal cells and glial cells distributed in the spinal ventral horn and brain stem, and also in the cytoplasm of ganglion cells in the spinal ganglion. No antigens were seen in the cerebral hemisphere. In the nervous system, the distribution of PTV antigens was consistent with lesions characteristic of nonsuppurative encephalomyelitis. In the other examined organ, PTV antigens were observed in bronchiolar epithelial cells in the lung, hepatocytes in the liver, epithelial cells in the tonsils and the myenteric nerve plexus in the small and large intestine.

  • immunohistochemical detection of Porcine Teschovirus antigen in the formalin fixed paraffin embedded specimens from pigs experimentally infected with Porcine Teschovirus
    Journal of Veterinary Medicine Series A-physiology Pathology Clinical Medicine, 2007
    Co-Authors: M. Yamada, Y Kaku, M Yoshii, Hiroshi Tsunemitsu, Y Yamamoto, K. Nakamura, A Miyazaki, M. Narita
    Abstract:

    Summary Porcine Teschovirus (PTV) antigens were detected by a streptavidin-biotin complex method in formalin-fixed paraffin-embedded tissues of 3-week-old pigs that had been inoculated intravenously with PTV Talfan strain. PTV antigens were detected in cytoplasm of nerve cells, glial cells and endothelial cells in the cerebellar nuclei, the grey matter of the midbrain, pons and medulla oblongata and the ventral horn of the spinal cord and of ganglion cells in the spinal ganglion corresponding to those lesions characterized as non-suppurative encephalomyelitis and ganglionitis. The results of this study suggest that nerve cells of the brain stem and spinal cord and ganglion cells of the spinal ganglion permit PTV replication and represent the main target cell population of PTV. This is the first study to demonstrate PTV antigen by immunohistochemistry in formalin-fixed paraffin-embedded tissue specimens from pigs infected with PTV.

  • Antigenic properties of Porcine Teschovirus 1 (PTV-1) Talfan strain and molecular strategy for serotyping of PTVs
    Archives of Virology, 2007
    Co-Authors: Y Kaku, Ai Sarai, Y Murakami, Y. Wang, S. Ohashi
    Abstract:

    For reliable diagnosis of Porcine Teschovirus (PTV) infection we created an RT-PCR-based molecular strategy for serotyping that encompassed the dominant neutralizing antigenic site of PTV, followed by phylogenetic analyses of amplicons. We identified neutralizing antigenic sites of PTV-1 Talfan strain through epitope mapping of neutralizing monoclonal antibodies (MAbs), using synthetic peptides spanning the capsid proteins. All 11 MAbs obtained recognized peptides in the EF loop (“puff” of VP2 protein. Two MAbs concurrently reacted to peptides, one in the GH loop of VP1 and one in the VP1 C terminus. Three-dimensional modeling of Talfan capsid protein predicted exposure of all these sites on the virion surface in a close line centered around puff. We then designed a single pair of degenerate primers to VP2 and amplified the region of ∼320 bp encompassing puff in 8 PTV prototype strains and 6 field isolates. Phylogenetic analyses of the puff sequences of 11 prototype strains and 34 field isolates obtained from databanks showed that all homotypic strains (both field and prototype) were always monophyletic, except for one ‘untypable’ Japanese strain. This RT-PCR-based strategy appears to be a reliable surrogate for serotyping and could facilitate the diagnosis and epidemiological study of PTV infection.

  • functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus Porcine Teschovirus 1 talfan
    Journal of Virology, 2006
    Co-Authors: Louisa S. Chard, Y Kaku, Barbara Jones, Arabinda Nayak, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) of Porcine Teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

Roland Zell - One of the best experts on this subject based on the ideXlab platform.

  • Single virus detection by means of atomic force microscopy in combination with advanced image analysis.
    Journal of Structural Biology, 2014
    Co-Authors: Thomas Bocklitz, Evelyn Kämmer, Stephan Stöckel, Karina Weber, Roland Zell, Volker Deckert, Dana Cialla, Jürgen Popp
    Abstract:

    Abstract In the present contribution virions of five different virus species, namely Varicella-zoster virus , Porcine Teschovirus , Tobacco mosaic virus , Coliphage M13 and Enterobacteria phage PsP3 , are investigated using atomic force microscopy (AFM). From the resulting height images quantitative features like maximal height, area and volume of the viruses could be extracted and compared to reference values. Subsequently, these features were accompanied by image moments, which quantify the morphology of the virions. Both types of features could be utilized for an automatic discrimination of the five virus species. The accuracy of this classification model was 96.8%. Thus, a virus detection on a single-particle level using AFM images is possible. Due to the application of advanced image analysis the morphology could be quantified and used for further analysis. Here, an automatic recognition by means of a classification model could be achieved in a reliable and objective manner.

  • phylogeny and evolution of Porcine Teschovirus 8 isolated from pigs in china with reproductive failure
    Archives of Virology, 2012
    Co-Authors: Roland Zell
    Abstract:

    A Porcine Teschovirus (PTV) was isolated from a dead piglet from a herd of 200 sows showing reproductive failure in Fuyu, Heilongjiang Province, China. Sequencing of most of the genome of this isolate, designated Fuyu/2009, revealed that is was a PTV-8 isolate, closely related to a previously identified Chinese PTV-8 strain (Jilin/2003). Both Chinese strains vary from the European PTV-8 strains by different clustering of the 3CD sequences. Infection with these viruses may be associated with pronounced diseases.

  • isolation and characterization of the first chinese strain of Porcine Teschovirus 8
    Journal of Virological Methods, 2010
    Co-Authors: Chaofan Zhang, Shouping Hu, Zhuo Zhang, Roland Zell
    Abstract:

    Investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in Jilin Province, northern China. Only Porcine Teschovirus (PTV, designated as PTV-8 Jilin/2003) was isolated from samples of organs. The presence of PTV was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (IPMA), polymerase chain reaction, and electron microscopy. Other pathogenic agents causing diarrhea, respiratory distress, and death (including Porcine rotavirus, transmissible gastroenteritis virus of swine, Porcine epidemic diarrhea virus, classical swine fever virus, pseudorabies virus, Porcine circovirus, Porcine reproductive and respiratory syndrome virus, Japanese encephalitis virus, Mycoplasma, Leptospira, Streptococcus, Listeria, and Brucella species) were excluded as possible causal agents because they were not associated consistently with the disease of the pigs. PTV-8 Jilin/2003 was adapted to grow in swine primary kidney (PK-15) cells and in a swine testicular cell line (ST cells). When inoculated into healthy pigs, PTV-8 Jilin/2003 caused the same symptoms as those observed in the affected herd. It is concluded that PTV-8 Jilin/2003 was the causal agent of this disease.

  • linkage map of protein protein interactions of Porcine Teschovirus
    Journal of General Virology, 2005
    Co-Authors: Roland Zell, Simone Seitz, Andreas Henke, Thomas Munder, Peter Wutzler
    Abstract:

    A yeast two-hybrid study was conducted to catalogue the protein–protein interactions of the Porcine Teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as homo- and heterodimeric interactions of the 2B, 3CD and 3D proteins, several were not found previously. Among these is the binding of the leader protein L to the proteinases 3C and 3CD. Unlike the poliovirus 3C, the Teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L–3C, L–3CD and 3C–3CD.

  • Linkage map of protein-protein interactions of Porcine Teschovirus
    2005
    Co-Authors: Roland Zell, Simone Seitz, Andreas Henke, Thomas Munder, Peter Wutzler
    Abstract:

    A yeast two-hybrid study was conducted to catalogue the protein–protein interactions of the Porcine Teschovirus non-structural proteins. Five homodimer, three reciprocal heterodimer and four unidirectional heterodimer interactions were observed. While several interactions are similar to those described in previous studies using enteroviruses, such as homo- and heterodimeric interactions of the 2B, 3CD and 3D proteins, several were not found previously. Among these is the binding of the leader protein L to the proteinases 3C and 3CD. Unlike the poliovirus 3C, the Teschovirus 3C proteinase dimerizes and interacts with 2BC, 3CD and 3D. The strongest interactions were observed for L–3C, L–3CD and 3C–3CD. Picornaviruses comprise the genera Aphthovirus, Cardiovirus, Enterovirus, Erbovirus, Hepatovirus, Kobuvirus, Parechovirus, Rhinovirus and Teschovirus (Stanway et al., 2005). In addition, Porcine enterovirus 8 and some simian enteroviruses have recently been suggested to be closely related species of another new picornavirus genus (Krumbholz et al., 2002; Oberste et al., 2003). Picornaviruses are small nonenveloped

Louisa S. Chard - One of the best experts on this subject based on the ideXlab platform.

  • Hepatitis C virus-related internal ribosome entry sites are found in multiple genera of the family Picornaviridae.
    Journal of General Virology, 2006
    Co-Authors: Louisa S. Chard, Marie-eve Bordeleau, Jerry Pelletier, Junichi Tanaka, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) elements from Porcine enterovirus 8 and simian virus 2, two members of a proposed new genus within the family Picornaviridae, were characterized. These IRES elements, in common with the Porcine Teschovirus 1 IRES, were found to be related functionally and structurally to the IRES element from Hepatitis C virus, a member of the family Flaviviridae. Partial secondary structure predictions were derived and functional assays demonstrated that these IRES elements continued to be active when eIF4G was cleaved and when the activity of eIF4A was blocked.

  • functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus Porcine Teschovirus 1 talfan
    Journal of Virology, 2006
    Co-Authors: Louisa S. Chard, Y Kaku, Barbara Jones, Arabinda Nayak, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) of Porcine Teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

  • functional analyses of rna structures shared between the internal ribosome entry sites of hepatitis c virus and the picornavirus Porcine Teschovirus 1 talfan
    Journal of Virology, 2006
    Co-Authors: Louisa S. Chard, Barbara Jones, Arabinda Nayak, Yoshihiro Kaku, Graham J. Belsham
    Abstract:

    The internal ribosome entry site (IRES) of Porcine Teschovirus 1 (PTV-1), a member of the Picornaviridae family, is quite distinct from other well-characterized picornavirus IRES elements, but it displays functional similarities to the IRES from hepatitis C virus (HCV), a member of the Flaviviridae family. In particular, a dominant negative mutant form of eIF4A does not inhibit the activity of the PTV-1 IRES. Furthermore, there is a high level (ca. 50%) of identity between the PTV-1 and HCV IRES sequences. A secondary-structure model of the whole PTV-1 IRES has been derived which includes a pseudoknot. Validation of specific features within the model has been achieved by mutagenesis and functional assays. The differences and similarities between the PTV-1 and HCV IRES elements should assist in defining the critical features of this type of IRES.

  • functional and structural similarities between the internal ribosome entry sites of hepatitis c virus and Porcine Teschovirus a picornavirus
    Journal of Virology, 2004
    Co-Authors: Andrey V Pisarev, Louisa S. Chard, Y Kaku, Ivan N. Shatsky, Helen L Johns, Graham J. Belsham
    Abstract:

    Initiation of protein synthesis on picornavirus RNA requires an internal ribosome entry site (IRES). Typically, picornavirus IRES elements contain about 450 nucleotides (nt) and use most of the cellular translation initiation factors. However, it is now shown that just 280 nt of the Porcine Teschovirus type 1 Talfan (PTV-1) 5′ untranslated region direct the efficient internal initiation of translation in vitro and within cells. In toeprinting assays, assembly of 48S preinitiation complexes from purified components on the PTV-1 IRES was achieved with just 40S ribosomal subunits plus eIF2 and Met-tRNAiMet. Indeed, a binary complex between 40S subunits and the PTV-1 IRES is formed. Thus, the PTV-1 IRES has properties that are entirely different from other picornavirus IRES elements but highly reminiscent of the hepatitis C virus (HCV) IRES. Comparison between the PTV-1 IRES and HCV IRES elements revealed islands of high sequence identity that occur in regions critical for the interactions of the HCV IRES with the 40S ribosomal subunit and eIF3. Thus, there is significant functional and structural similarity between the IRES elements from the picornavirus PTV-1 and HCV, a flavivirus.

  • functional and structural similarities between the internal ribosome entry sites of hepatitis c virus and Porcine Teschovirus a picornavirus
    Journal of Virology, 2004
    Co-Authors: Andrey V Pisarev, Louisa S. Chard, Y Kaku, Ivan N. Shatsky, Helen L Johns, Graham J. Belsham
    Abstract:

    Initiation of protein synthesis on picornavirus RNA requires an internal ribosome entry site (IRES). Typically, picornavirus IRES elements contain about 450 nucleotides (nt) and use most of the cellular translation initiation factors. However, it is now shown that just 280 nt of the Porcine Teschovirus type 1 Talfan (PTV-1) 5′ untranslated region direct the efficient internal initiation of translation in vitro and within cells. In toeprinting assays, assembly of 48S preinitiation complexes from purified components on the PTV-1 IRES was achieved with just 40S ribosomal subunits plus eIF2 and Met-tRNAiMet. Indeed, a binary complex between 40S subunits and the PTV-1 IRES is formed. Thus, the PTV-1 IRES has properties that are entirely different from other picornavirus IRES elements but highly reminiscent of the hepatitis C virus (HCV) IRES. Comparison between the PTV-1 IRES and HCV IRES elements revealed islands of high sequence identity that occur in regions critical for the interactions of the HCV IRES with the 40S ribosomal subunit and eIF3. Thus, there is significant functional and structural similarity between the IRES elements from the picornavirus PTV-1 and HCV, a flavivirus.

Jana Prodelalova - One of the best experts on this subject based on the ideXlab platform.

  • Congenital Microphthalmic Syndrome in a Swine.
    Case reports in Veterinary Medicine, 2018
    Co-Authors: Radka Andrysikova, Dolf Kümmerlen, Wolfgang Pendl, Robert Graage, Romana Moutelikova, Jana Prodelalova, Titus Sydler, Katrin Voelter
    Abstract:

    A 17-week-old crossbred finishing pig was presented for lameness of approximately one week. Clinical evaluation, including ophthalmologic examination, revealed ataxia, partial flaccid paresis of the pelvic limbs, skin lesions at feet and claws, and severely reduced vision/blindness. Both eyes had multiple persistent pupillary membranes (iris-to-iris and iris-to-lens) and hypermature cataracts. Histopathological examination of the eyes revealed microphthalmia, microphakia with cataract formation, myovascularised membrane in the vitreous, retinal detachment, and retinal dysplasia. Microscopic examination of tissues collected postmortem demonstrated nonsuppurative polioencephalomyelitis with the most prominent inflammatory lesions in the lumbar spinal cord. Subsequently, presumed Teschen/Talfan disease was confirmed by Porcine Teschovirus identification in the spinal cord using the reverse transcription-polymerase chain reaction (RT-PCR). To the authors’ knowledge, this is the first case report describing in detail histopathological changes in the Porcine congenital microphthalmic syndrome.

  • genotyping of Porcine Teschoviruses isolated from 1960 to 1980 in the former czechoslovakia and new Porcine Teschovirus isolates obtained from piglets with diarrhoea
    Veterinarni Medicina, 2018
    Co-Authors: Jana Prodelalova, H Malenovska, L Valicek
    Abstract:

    The Porcine enteroviruses (family Picornaviridae) were described as causative agents of neurological disorders known as Teschen/Talfan disease, reproductive failure, dermal lesions, enteric disease and pneumonia of swine. Recently, Porcine enteroviruses were reclassified on the basis of genome sequencing data. A new Picor- navirus genus named Teschovirus (formerly PEV CPE group I.) was established. The aim of the study was retyping and reclassification of the 27 strains of Porcine enteroviruses deposited in the Collection of Animal Pathogenic Microorganisms (CAPM). Viral strains were isolated over the period 1960-1980 predominantly from pigs with encephalomyelitis. Twenty-four of 27 isolates were classified as Porcine enterovirus serotype 1 (PEV-1) based on physicochemicals properties of their virions and growth characteristics. The viral strains were reclassified using RT-PCR protocol that allowed detection of the genus Teschovirus and the Porcine Teschovirus serotype 1 (PTV-1). Two PCR amplifications with cDNA were performed for detection of the genus Teschovirus and PTV-1. Amplification of fragment characteristic for the genus eschovirus was succesful for all tested viral strains. The fragment characteristic for PTV-1 was detected with the exception of three strains (Kr69TK, 95 and 172). Used RT-PCR method was subsequently applied to the detection of Porcine Teschovirus in pig fecal samples. Ten of 22 faecal samples were found to be Porcine Teschovirus positive, however none of 22 samples generated amplicon specific for the PTV-1.

  • comparative inactivation of aujeszky s disease virus Porcine Teschovirus and vesicular stomatitis virus by chemical disinfectants
    Veterinarni Medicina, 2018
    Co-Authors: H. Dvorakova, Jana Prodelalova, M. Reichelova
    Abstract:

    We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (car - rier test). The agents of the Porcine encephalomyelitis ( Porcine Teschovirus, strains CAPM V-86, CAPM V-37), Aujeszky's disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the Porcine Teschovirus was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard. The other disinfectants decreased the viral titre insufficiently. Under the same conditions, Aujeszky's disease virus was inactivated by at least 4 lg by all the tested disinfectants except for Chloramin BM which decreased the titre of CAPM V-166 only by 3.75 lg in the carrier test. For the inactivation of Vesicular stomatitis virus Chloramin BM and Mikasept KP were tested. Both the disinfectants reliably decreased the viral titre in both the suspension and carrier tests. Our results show that the inactivation of a surface-bound virus is more difficult than its inactivation in suspension. We confirm the high resistance of non-enveloped viruses ( Porcine tescho- virus) to chemical inactivation.

  • the survey of Porcine Teschoviruses sapeloviruses and enteroviruses b infecting domestic pigs and wild boars in the czech republic between 2005 and 2011
    Infection Genetics and Evolution, 2012
    Co-Authors: Jana Prodelalova
    Abstract:

    This study presents results of epidemiological survey and genetic characterisation of Porcine enteric picornaviruses belonging to the genera Teschovirus, Sapelovirus, and Porcine enterovirus B. Faecal or gut content samples from domestic pigs (Sus scrofa f. domestica) and the cecal content of wild boars (Sus scrofa) of different ages (collected between 2005 and 2011) were analysed by molecular methods. Porcine enterovirus B was the most prevalent virus detected in both domestic pigs and wild boars (50.2% and 69.4%, respectively), followed by Porcine Teschovirus and Porcine sapelovirus. The majority of positive domestic pigs (69.4%) and wild boars (64.3%) were infected with two or three tested viruses. There was no significant difference in prevalences of Teschoviruses, sapeloviruses, and enteroviruses among healthy and diarrhoeic pigs. Results of epidemiological survey demonstrated that all target viral genera are common in Czech farms producing pigs and wild boars. Amplified nucleotide fragments of VP2 region obtained from randomly selected both historical and recent Teschovirus isolates were sequenced. Based on sequence data, historical Porcine Teschovirus isolate CAPM V-180, previously determined as serotype 1 was reclassified into serotype 11. Moreover, another recent Porcine Teschovirus isolate OH264/2010 was described and classified into serotype 11. Four nontypeable PTV strains (historical isolate CAPM V-182/1976 and recent isolates JA247/2010, NI429/2010, and BR1576/2007) identified in this study might represent novel serotypes. To the best of our knowledge, our study represents the first description of this serotype in the Czech Republic.

  • Comparative inactivation of Aujeszky’s disease virus, Porcine Teschovirus and Vesicular stomatitis I by chemical disinfectants
    Veterinární Medicína, 2008
    Co-Authors: H. Dvorakova, Jana Prodelalova, M. Reichelova
    Abstract:

    We tested the germicide activity of 1% Chloramin BM, 1% Incidin Plus, 1% Lysoformin 3000, 0.2% Mikasept KP, and 2% Sekusept Forte against viruses in suspension (suspension test) and dried onto a surface (carrier test). The agents of the Porcine encephalomyelitis (<I>Porcine Teschovirus</I>, strains CAPM V-86, CAPM V-37), Aujeszky’s disease (strains CAPM V-166, CAPM V-327) and vesicular stomatitis (strains CAPM V-499, CAPM V-331) were used as model viruses. After 30 min contact time in both the suspension and carrier tests, the <I>Porcine Teschovirus</I> was 4 lg inactivated only by Mikasept, which was thus the only disinfectant to meet the standard. The other disinfectants decreased the viral titre insufficiently. Under the same conditions, <I>Aujeszky’s disease virus</I> was inactivated by at least 4 lg by all the tested disinfectants except for Chloramin BM which decreased the titre of CAPM V-166 only by 3.75 lg in the carrier test. For the inactivation of <I>Vesicular stomatitis virus </I>Chloramin BM and Mikasept KP were tested. Both the disinfectants reliably decreased the viral titre in both the suspension and carrier tests. Our results show that the inactivation of a surface-bound virus is more difficult than its inactivation in suspension. We confirm the high resistance of non-enveloped viruses (<I>Porcine Teschovirus</I>) to chemical inactivation.