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J Chen - One of the best experts on this subject based on the ideXlab platform.
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a new Potyvirus from thunberg fritillary fritillaria thunbergii miq in zhejiang china
Archives of Virology, 2005Co-Authors: J Chen, Q Y Zhang, H Y Zheng, M J Adams, J P ChenAbstract:A Potyvirus causing mosaic symptoms in Thunberg fritillary (Fritillaria thunbergii) was found at two sites in Zhejiang province, China. The virus was readily mechanically transmitted to its original host but not to any of 17 other widely used plant virus indicators. A polyclonal antiserum raised to purified virus particles reacted with its homologous virus but not with a range of other viruses (including 16 Potyvirus species). In electron microscopy, virus particles and inclusion bodies typical of a Potyvirus were seen. The complete nucleotide sequence of an isolate from Ningbo was determined. It was 9723 nt long and sequence analyses predicted the standard Potyvirus organisation. The partial sequence (1664 nts at the 3′-terminus) of an isolate from Panan was also determined; the two sequences had 96.9% nt identity. In sequence comparisons and phylogenetic analyses with completely sequenced Potyviruses, the new virus was most closely related to Lily mottle virus (53.0% aa identity) and Leek yellow stripe virus. The most closely related incomplete sequence in the international databases was for Lycoris mild mottle virus (72.8% nt identity in their coat proteins). These results suggest that the virus studied is a new species in the genus Potyvirus, which we have tentatively named Thunberg fritillary mosaic virus.
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A new Potyvirus from tuberose ( Polianthes tuberosa) in China.
Archives of Virology, 2004Co-Authors: H Y Zheng, J Chen, Q Y Zhang, J P Chen, M.-f. Zhao, John F. Antoniw, M J AdamsAbstract:Tuberose plants with mild mottle symptoms, growing in a glasshouse in Hangzhou, China, contained virions and inclusion bodies typical of a Potyvirus. The virus was mechanically transmitted to tuberose but not to 14 other test plant species. A fragment of 4607 nucleotides, corresponding to the 3′-half of a typical Potyvirus was amplified by RT-PCR using degenerate primers and sequenced. The most similar sequence in the databases was that of Tuberose mild mosaic virus (TuMMV) from Taiwan and this was the only virus significantly related to it in phylogenetic analyses. The new sequence had 71.1% nt and 76.6% aa identity to TuMMV in the coat protein. Western blot analyses using antisera raised to expressed coat protein showed that the two viruses were serologically related. Although there are no substantial biological data to distinguish the Hangzhou isolate from TuMMV, the molecular difference between the two virus isolates is similar to, or slightly greater than, that between several pairs of well-established Potyvirus species. These results therefore suggest that the Hangzhou isolate should be regarded as a new member of the genus Potyvirus, and we have tentatively named it Tuberose mild mottle virus.
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molecular characterisation of an isolate of dasheen mosaic virus from zantedeschia aethiopica in china and comparisons in the genus Potyvirus
Archives of Virology, 2001Co-Authors: Jiong Chen, J Chen, Jishuang Chen, M J AdamsAbstract:The complete nucleotide sequence of an isolate of Dasheen mosaic virus from Zantedeschia aethiopica in Zhejiang Province, China, was determined. The 9991 nucleotide genome was typical of the genus Potyvirus and phylogenetic analysis showed it to be a member of the Bean common mosaic virus subgroup. The 3′-terminal sequence, including the coat protein region, was determined for three further isolates from China and Japan. Variations in the length and composition of the N-terminus of the coat protein were not related to geographic origin or plant host. An analysis of all Potyvirus cleavage sites revealed patterns related to phylogenetic groupings.
R A A Van Der Vlugt - One of the best experts on this subject based on the ideXlab platform.
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pepper yellow mosaic virus a new Potyvirus in sweetpepper capsicum annuum
Archives of Virology, 2002Co-Authors: Alice K Inouenagata, A. N. Dusi, M E N Fonseca, R O Resende, L S Boiteux, Damares C Monte, A C De Avila, R A A Van Der VlugtAbstract:A Potyvirus was found causing yellow mosaic and veinal banding in sweetpepper in Central and Southeast Brazil. The sequence analysis of the 3′ terminal region of the viral RNA revealed a coat protein of 278 amino acids, followed by 275 nucleotides in the 3′-untranslated region preceding a polyadenylated tail. The virus shared 77.4% coat protein amino acid identity with Pepper severe mosaic virus, the closest Potyvirus species. The 3′-untranslated region was highly divergent from other Potyviruses. Based on these results, the virus found in sweetpepper plants could be considered as a new Potyvirus. The name Pepper yellow mosaic virus (PepYMV) is suggested.
H Nakamae - One of the best experts on this subject based on the ideXlab platform.
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complete nucleotide sequence of the genomic rna of a japanese yam mosaic virus a new Potyvirus in japan
Archives of Virology, 1999Co-Authors: S Fuji, H NakamaeAbstract:We determined the complete nucleotide sequence of a Potyvirus purified from a Japanese yam plant. The genomic RNA of this virus is 9 757 nucleotides (nts) in length, excluding the 3′-terminal poly(A) tail. It contains a single open reading frame (ORF) encoding a polyprotein of 3130 amino acids (aa) with a calculated Mr of 356,793. The genomic organization of this Potyvirus is similar to that of other members of the genus Potyvirus and nine potential cleavage sites for the viral proteinase were found by comparison of its sequence with those available for other Potyviruses. The nucleotide sequence and genome characteristics show that this isolate is a new Potyvirus species. Its polyprotein differs substantially from Yam mosaic virus (YMV) (50% amino acid sequence identity) and fourteen other Potyvirus species examined (44–59% identity). Although this Potyvirus has been classified as YMV, our results suggest that the Potyvirus infectious to the Japanese yam plant in Japan is distinct from YMV. Therefore, we propose that the Japanese yam Potyvirus should be designated as Japanese yam mosaic virus (JYMV).
Rebecca Grumet - One of the best experts on this subject based on the ideXlab platform.
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biological and molecular characterization of moroccan watermelon mosaic virus and a Potyvirus isolate from eastern sudan
Plant Disease, 2001Co-Authors: H Lecoq, Cécile Desbiez, G Dafalla, C Wipfscheibel, B Delecolle, Tanya Lanina, Zakir Ullah, Rebecca GrumetAbstract:A Potyvirus (Su-94-54) was isolated from a naturally infected snake cucumber (Cucumis melo var. flexuosus) plant with severe mosaic and leaf deformation symptoms collected in Eastern Sudan. This isolate has a host range limited to cucurbits and is serologically distantly related to Moroccan watermelon mosaic virus (MWMV) and to Papaya ringspot virus (PRSV). Coat protein sequence analysis of Su-94-54 and MWMV and comparison with other Potyviruses indicate that Su-94-54 is more closely related to MWMV than to any other Potyvirus. Based on the amino acid sequence identity in the core part of the coat protein with MWMV (86%), this isolate could be regarded as a distinct species. However, because of biological, cytological, and serological affinities with MWMV, we propose that this isolate be considered as a strain of MWMV, possibly an evolutionary intermediate between MWMV and PRSV, until more is known on the structure of the PRSV subgroup within the genus Potyvirus.
Dharma D. Shukla - One of the best experts on this subject based on the ideXlab platform.
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Coat protein sequence of Krish-infecting strain of Johnsongrass mosaic Potyvirus
Archives of Virology, 1998Co-Authors: Suranto, Dharma D. Shukla, K. H. Gough, C. K. PallaghyAbstract:The morphology of an Australian strain of Johnsongrass mosaic Potyvirus (JGMV Krish-infecting strain), capable of infecting sorghums carrying the Krish Potyvirus resistance gene, was investigated and the base sequence of the coat protein region determined. Under the electron microscope the virus was indistinguishable from the wild type prevalent in Australia, JGMV-Jg. However, there were some significant changes in the inferred amino acid sequence in both the N-terminus and the core regions of the coat protein. Some of these amino acid changes may be responsible for breaking the resistance of sorghums carrying the Krish virus resistance gene. In the discussion mention is made of a preliminary result with a mutated in vitro transcript which supports this suggestion.
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Chimeric Potyvirus-like particles as vaccine carriers.
Intervirology, 1996Co-Authors: Mittur N. Jagadish, Dharma D. Shukla, S. J. Edwards, M. B. Hayden, R.c. Hamilton, Julian Grusovin, Kirsten Vandenberg, Peter Schoofs, H. Kalnins, M. McnamaraAbstract:Presentation of subunit vaccines in a highly ordered aggregate form can result in enhanced immune responses. Coat protein (CP) monomers of a Potyvirus (Johnsongrass mosaic virus) when produced in hete
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Watermelon mosaic virus-Morocco is a distinct Potyvirus.
Archives of Virology, 1993Co-Authors: Neil M. Mckern, P. M. Strike, O. W. Barnett, Colin W. Ward, Dharma D. ShuklaAbstract:The relationship of the Morocco isolate of watermelon mosaic virus (WMV) to WMV 2, soybean mosaic virus (a virus closely related to WMV 2) and the W strain of papaya ringspot virus (PRSV-W), formerly WMV 1, was examined by comparing tryptic peptide profiles using high performance liquid chromatography. The profiles indicated that the coat protein sequence of WMV-Morocco differed substantially from those of the other Potyviruses. This conclusion was supported by sequence data from five tryptic peptides from the coat protein of WMV-Morocco, which showed only 61–68% identity to equivalent sequences in PRSV-W, WMV 2 and zucchini yellow mosaic, another Potyvirus infecting cucurbits. Based on the above data, and on known correlations between coat protein sequence similarities and Potyvirus relationship, it is concluded that WMV-Morocco should be regarded as a distinct Potyvirus.
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Strains of Peanut Stripe Potyvirus Rapidly Identified by Profiling Peptides of the Virion Proteins
Journal of Phytopathology, 1993Co-Authors: Kruapan Kittipakorn, Neil M. Mckern, Adrian J. Gibbs, Dharma D. ShuklaAbstract:Virion proteins of five Potyvirus isolates from groundnut in Thailand which induce a range of symptoms were compared with those from the type strains of peanut stripe Potyvirus (PStV) and peanut mottle Potyvirus (PeMoV). Profiles obtained by high performance liquid chromatography (HPLC) of tryptic peptide digests of the virion proteins of all five Thai isolates and PStV were very similar, whereas that of PeMoV was quite different. The results indicate that the Thai isolates are strains of PStV, and reaffirm the value of HPLC peptide profiling for rapidly identifying Potyviruses.
