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Kamran Fakhimzadeh - One of the best experts on this subject based on the ideXlab platform.

  • Physical control of varroa mites (Varroa destructor): the effects of various dust materials on varroa mite fall from adult honey bees (Apis mellifera) in vitro
    Journal of Apicultural Research, 2011
    Co-Authors: Kamran Fakhimzadeh, James D Ellis, Jerry Hayes
    Abstract:

    SummaryIn this study, we investigated the impact of ten different materials on the fall of varroa (Varroa destructor) mites from adult honey bees (Apis mellifera) in vitro. To do this, we confined ∼250 adult bees each to 144, 0.47 l glass jars, feeding them bee candy (honey/Powdered Sugar) ad lib. Seventy-two hours later, the bees were treated with 5 g of one of the ten materials and placed in one of two locations (in an incubator = 35°C, 70% RH, or in a dark room = 22°C, 50% RH; replicate schedule = 10 dusts and 2 controls x 6 jars/rep x 2 locations). Immediately after and 24 h after treatment application, we counted the number of mites that fell from the jar through the mesh lid when the jar was rolled for 30 s, inverted, and shaken for 30 s. Four days later, we estimated the number of bees alive in each jar and washed the bees to remove any remaining mites. With these data, we determined the mite drop efficacy of the materials and their acute impact on adult bee survivorship. In general, Powdered Sugar...

  • Does Powdered Sugar Work as a Varroa Control
    Bee World, 2010
    Co-Authors: Kamran Fakhimzadeh
    Abstract:

    During his placement at Florida University Dr Fakhimzadeh has been investigating the effectiveness of dusting bees with Sugar powder as a form of Varroa control. Here he attempts to resolve the beekeepers' dilemma of whether it is a feasible solution or not.

  • The effects of Powdered Sugar varroa control treatments on Apis mellifera colony development
    Journal of Apicultural Research, 2001
    Co-Authors: Kamran Fakhimzadeh
    Abstract:

    Dusting colonies with Powdered Sugar, wheat flour or other dust materials for the control of varroa have been reported in the apicultural literature. High levels of mite drop have been obtained by Sugar dusting adult bees. It was also shown that in dusting adult bees, no Sugar particles entered the T2 spiracles and their tracheal ducts. In the present study, the impact of Powdered Sugar dusting on the number of both capped brood cells and adult bees was studied. During July in a Finnish apiary, 12 honey bee (Apis mellifera) colonies were dusted with 10-20 g of Powdered Sugar at 3-, 7- and 14-day intervals. Another 6 colonies were left untreated as control colonies. An additional 6 colonies treated with fluvalinate (during the previous autumn) formed the chemically treated control. Number of capped brood and adult bees were recorded during the summer. There were no significant differences among the Sugar-treated and untreated control groups in both the number of capped brood cells (P = 0.168) and additional number of adult bees (population growth, P = 0.78), 18 days after the start of the experiment. Data analyses demonstrated that there is no obvious side effect of dusting on colony development. No queen supersedure occurred during July in the colonies under Sugar treatment.

Aleš Gregorc - One of the best experts on this subject based on the ideXlab platform.

  • Diagnosis of Varroa Mite (Varroa destructor) and Sustainable Control in Honey Bee (Apis mellifera) Colonies—A Review
    Diversity, 2019
    Co-Authors: Aleš Gregorc, Blair J. Sampson
    Abstract:

    Determining varroa mite infestation levels in honey bee colonies and the proper method and time to perform a diagnosis are important for efficient mite control. Performing a Powdered Sugar shake or counting mites that drop from combs and bees onto a hive bottom board are two reliable methods for sampling varroa mite to evaluate the efficacy of an acaricide treatment. This overview summarizes studies that examine the efficacy of organic acids and essential oils, mite monitoring, and brood interruption for integrated varroa mite control in organic beekeeping.

  • Powdered Sugar shake to monitor and oxalic acid treatments to control varroa mites (Varroa destructor Anderson and Trueman) in honey bee (Apis mellifera) colonies
    Journal of Apicultural Research, 2017
    Co-Authors: Aleš Gregorc, Patricia R. Knight, John J. Adamczyk
    Abstract:

    A practical method to control the destructive varroa mite, Varroa destructor Anderson and Trueman in honey bee colonies consisted of monitoring with Powdered Sugar and treatment with oxalic acid (OA). We tested mite populations within 28 honey bee colonies on adult bees by a using a Powdered Sugar shake method. Subsequently, the same colonies were treated with 2.9% oxalic acid and 31.9% sucrose-in-water solution (w/w) four times, with a final varroa treatment using Apistan strips. On average 66.10 ± 35.23% and 94.64 ± 9.56% mites were detected using this simple Sugar shake method in August and October, 2015. Furthermore, positive correlations were found between relative number of infested honey bees detected by Powdered Sugar shake and washing bees with alcohol plus soapy water (r = 0.90 and r = 0.62). Temperature and humidity seems to be crucial in determining the effectiveness of the Powdered Sugar shake method. For control of the mites, four consecutive OA treatments performed during the winter months ...

Neena Singla - One of the best experts on this subject based on the ideXlab platform.

  • effect of nifedipine on fertility of male lesser bandicoot rat bandicota bengalensis
    Applied Biological Research, 2017
    Co-Authors: Neena Singla, Parul Dhar, Kuldeep Gupta
    Abstract:

    Current study was designed to evaluate the antifertility effects of nifedipine, a pharmaceutical medicament for high blood pressure. Mature and healthy male lesser Bandicoot rats, Bandicota bengalensis Gray and Hardwicke, were fed on bait (cracked wheat, Powdered Sugar, groundnut oil 98:2:2) containing 0.1 and 0.05% nifedipine for 12 days in no-choice feeding test to record antifertility effects of nifedipine. The factors studied were the effect on weight of reproductive organs, sperm parameters in cauda epididymal fluid, breeding performance and histomorphology of testis. Nifedipine treatment significantly (P <0.05) reduced the weight of seminal vesicles and prostate gland in treated groups as compared to untreated group. Sperm motility, live sperm count, sperm density and sperm morphology differed significantly between untreated and treated groups. The breeding performance of treated male rats was found reduced by 40–60% as compared to untreated ones. A significant effect of nifedipine treatment was observed on the number of germinal cells in seminiferous tubules. The study indicates the effect of nifedipine on fertility of male rats.

  • Effect of nifedipine on fertility of male lesser Bandicoot rat (Bandicota bengalensis)
    Applied Biological Research, 2017
    Co-Authors: Neena Singla, Parul Dhar, Kuldeep Gupta
    Abstract:

    Current study was designed to evaluate the antifertility effects of nifedipine, a pharmaceutical medicament for high blood pressure. Mature and healthy male lesser Bandicoot rats, Bandicota bengalensis Gray and Hardwicke, were fed on bait (cracked wheat, Powdered Sugar, groundnut oil 98:2:2) containing 0.1 and 0.05% nifedipine for 12 days in no-choice feeding test to record antifertility effects of nifedipine. The factors studied were the effect on weight of reproductive organs, sperm parameters in cauda epididymal fluid, breeding performance and histomorphology of testis. Nifedipine treatment significantly (P

  • Anti-Reproductive effects of total glycosides of Tripterygium wilfordii in male house rat, Rattus rattus
    Applied Biological Research, 2015
    Co-Authors: Neena Singla
    Abstract:

    Present study was conducted to evaluate anti-reproductive effects of total glycosides of Tripterygium wilfordii (GTW) (Thunder God Vine), in male House rat, Rattus rattus, a predominant rodent pest species found in commensal situations in Punjab, India. Mature male rats were fed on bait (cracked wheat and Powdered Sugar, 98: 2) containing 0.1, 0.2 and 0.3% GTW for 10 days in no-choice feeding tests. Results revealed 67.285.2% acceptability of treated bait over untreated bait. Autopsy of rats after 30 days of treatment withdrawal revealed significant (P≤0.05) reduction in weights of testes and epididymides. The sperm motility, live sperm count and sperm concentration in the cauda epididymal fluid of treated rats was also reduced significantly compared to that in untreated rats. The major abnormality in sperm morphology was sperm head tail separation. Results suggest the use of GTW for reducing reproduction of R.rattus in integration with chemical control.

  • histomorphological and biochemical changes induced by triptolide treatment in male lesser bandicoot rat bandicota bengalensis
    Pesticide Biochemistry and Physiology, 2014
    Co-Authors: Parul Dhar, Neena Singla
    Abstract:

    Mature and healthy male lesser bandicoot rats, Bandicota bengalensis (n = 40) were fed on bait (mixture of cracked wheat and Powdered Sugar in 98:2) containing different concentrations of triptolide (0, 0.15, 0.20 and 0.25% w/w) for 15 days in two-choice trials. Results revealed no significant effect of triptolide treatment on weights of vital organs after 30 and 60 days of treatment withdrawal. A significant (P ≤ 0.05) increase in plasma levels of TP, ALP, ACP, ALT and AST in response to stress induced in groups of rats treated with 0.20 and 0.25% triptolide was observed after 30 days of treatment withdrawal. No significant effect of treatment was observed on histomorphology of liver. A significant (P ≤ 0.05) effect of triptolide treatment was, however, observed on testicular function in the form of reduced diameter of seminiferous tubules and number of various spermatogenic cells indicating effect on spermatogenesis and spermiogenesis. The cell stages affected did not recover fully within 60 days period following treatment withdrawal. The present study suggests the potential of triptolide in the reproductive management of B. bengalensis by way of affecting testicular function.

  • effect of crude cottonseed oil containing gossypol on fertility of male and estrous cycle of female bandicota bengalensis gray and hardwicke
    Journal of Applied Animal Research, 2013
    Co-Authors: Neena Singla, Meenakshi Garg
    Abstract:

    Crude cottonseed oil (CCSO) containing 0.01% gossypol was evaluated in the present studies to record its effect on fertility of male and estrous cycle of female lesser bandicoot rat, Bandicota bengalensis Gray and Hardwicke. Laboratory feeding of bait (cracked wheat and Powdered Sugar in 98:2) containing 5 and 10% CCSO to female B. bengalensis for 13 days in no-choice feeding test, had no significant effect on the duration of the estrous cycle. However, laboratory feeding of bait containing 10% CCSO to male B. bengalensis for 15 and 30 days duration in a no-choice feeding test, significantly (P≤0.05) reduced sperm motility (%) and viability (%) in cauda epididymal fluid of treated rats compared to untreated ones. The weight of seminal vesicles of rats treated for 30 days was found to be significantly less (P≤0.05) than that for rats treated for 15 days. No significant effect of treatment was observed on the breeding success of rats, diameter of seminiferous tubules, cell count and association of various g...

Marion D Ellis - One of the best experts on this subject based on the ideXlab platform.

  • only large amounts of Powdered Sugar applied directly to brood cells harms immature honey bees
    Journal of Apicultural Research, 2005
    Co-Authors: Nicholas P Aliano, Marion D Ellis
    Abstract:

    SUMMARYWe applied measured amounts of Powdered Sugar directly to honey bee brood combs containing eggs and larvae of known age groups. 24 h later, we compared Powdered Sugar-treated brood with similarly aged cohorts of immature bees that did not receive Powdered Sugar. Low and high doses of Powdered Sugar (0.3 and 0.6 g per 151.5 ± 1.0 cells, respectively) caused significant egg removal of 62.2 ± 5.1% and 86.1± 5.1%, respectively, when compared to eggs that received no Powdered Sugar (t = 16.91; df = 29; P = 0.0001). Powdered Sugar had no effect on 5-day-old honey bee larvae (t = 0.74; df = 29; P = 0.4668), but caused a significant percentage of 8-day-old larvae (18.5 ± 4.5%) to be removed when applied at the high dose (t = 4.10; df = 29; P = 0.0003).

  • A strategy for using Powdered Sugar to reduce varroa populations in honey bee colonies
    Journal of Apicultural Research, 2005
    Co-Authors: Nicholas P Aliano, Marion D Ellis
    Abstract:

    We developed a technique to remove varroa (Varroa destructor) from adult honey bees (Apis mellifera) using Powdered Sugar. Our technique requires isolating a colony's adult bee population in a deta...

  • Using inert dusts to detect and assess varroa infestations in honey bee colonies
    Journal of Apicultural Research, 2002
    Co-Authors: P. A. Macedo, Marion D Ellis
    Abstract:

    SUMMARYDetection and assessment of varroa infestations in honey bee colonies are important for successful beekeeping. We examined the use of inert dusts to dislodge mites from adult honey bees that were isolated from their nest. Six dusts (Powdered Sugar, fine Sugar, wheat flour, talcum powder, corn starch and baking soda) were evaluated for their ability to dislodge mites from adult bees collected in jars. We obtained the highest recovery rate with Powdered Sugar (92.9 ± 5.5%) and talcum powder (84.0 ± 5.6%). We also examined mite survival after recovery with inert dusts and compared it to mite survival after recovery from brood. After 24 h mite survival was significantly greater when mites were recovered with corn starch, Powdered Sugar, and from brood (F = 22.88, d.f. = 6,35, P < 0.0001). Finally, ether and Powdered Sugar were compared as tools for detecting and assessing the degree of infestation. Powdered Sugar did not differ from ether in detecting or assessing low (1–5 mites per sample) infestation...

John J. Adamczyk - One of the best experts on this subject based on the ideXlab platform.

  • Powdered Sugar shake to monitor and oxalic acid treatments to control varroa mites (Varroa destructor Anderson and Trueman) in honey bee (Apis mellifera) colonies
    Journal of Apicultural Research, 2017
    Co-Authors: Aleš Gregorc, Patricia R. Knight, John J. Adamczyk
    Abstract:

    A practical method to control the destructive varroa mite, Varroa destructor Anderson and Trueman in honey bee colonies consisted of monitoring with Powdered Sugar and treatment with oxalic acid (OA). We tested mite populations within 28 honey bee colonies on adult bees by a using a Powdered Sugar shake method. Subsequently, the same colonies were treated with 2.9% oxalic acid and 31.9% sucrose-in-water solution (w/w) four times, with a final varroa treatment using Apistan strips. On average 66.10 ± 35.23% and 94.64 ± 9.56% mites were detected using this simple Sugar shake method in August and October, 2015. Furthermore, positive correlations were found between relative number of infested honey bees detected by Powdered Sugar shake and washing bees with alcohol plus soapy water (r = 0.90 and r = 0.62). Temperature and humidity seems to be crucial in determining the effectiveness of the Powdered Sugar shake method. For control of the mites, four consecutive OA treatments performed during the winter months ...