The Experts below are selected from a list of 993 Experts worldwide ranked by ideXlab platform
Rachna Singh - One of the best experts on this subject based on the ideXlab platform.
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decolourisation and detoxification of rayon grade pulp paper mill effluent by mixed bacterial culture isolated from pulp paper mill effluent polluted site
Biochemical Engineering Journal, 2012Co-Authors: Ram Chandra, Rachna SinghAbstract:Abstract This study deals with the decolourisation and detoxification of rayon grade pulp paper mill effluent by mixed culture of three bacterial strains having ligninolytic enzyme activity. These strains were identified as IITRP1 Pseudochrobactrum glaciale (FJ581024), IITRP2 Providencia rettgeri (GU193984) and RCT2 Pantoea sp. (FJ755943) based on 16S rRNA gene sequencing. The results showed that mixed culture effectively reduced colour 96.02%, chemical oxygen demand (COD) 91% and biological oxygen demand (BOD) 92.59% of the pulp paper mill effluent within 216 h of the incubation period. Maximum enzyme activity for lignin peroxidase (LiP), manganese peroxidase (MnP) and laccase were recorded at 48, 72 and 144 h of the incubation period, respectively. The high performance liquid chromatography (HPLC) analysis of bacterial degraded samples showed 90% reduction of lignin and chlorophenol compared to the untreated sample. Further, gas chromatography–mass spectroscopy (GC–MS) analysis revealed that majority of the compounds present in the untreated sample were completely removed and only few metabolites were generated after bacterial treatment. The toxicity assessment of the pulp paper mill effluent on Vicia faba L. showed 40% reduction after bacterial treatment. However, untreated effluent sample showed inhibition of seed germination and α-amylase activity.
Ligia Salgueiro - One of the best experts on this subject based on the ideXlab platform.
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chemical composition and antibacterial activity of lavandula coronopifolia essential oil against antibiotic resistant bacteria
Natural Product Research, 2015Co-Authors: Ait L Said, Kawtar Zahlane, I Ghalbane, El S Messoussi, Abderrahmane Romane, Carlos Cavaleiro, Ligia SalgueiroAbstract:The aim of this study was to analyse the composition of the essential oil (EO) of Lavandula coronopifolia from Morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. The antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: Klebsiella pneumoniae subsp. pneumoniae, Klebsiella ornithinolytica, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Providencia rettgeri, Citrobacter freundii, Hafnia alvei, Salmonella spp., Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus. The main compounds of the oil were carvacrol (48.9%), E-caryophyllene (10.8%) and caryophyllene oxide (7.7%). The oil showed activity against all tested strains with minimal inhibitory concentration (MIC) values ranging between 1% and 4%. For most of the strains, the MIC value was equivalent to the minimal bactericidal concentration value, indicatin...
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Chemical composition and antibacterial activity of Lavandula coronopifolia essential oil against antibiotic-resistant bacteria
2015Co-Authors: Ait L Said, Kawtar Zahlane, I Ghalbane, El S Messoussi, Abderrahmane Romane, Carlos Cavaleiro, Ligia SalgueiroAbstract:The aim of this study was to analyse the composition of the essential oil (EO) of Lavandula coronopifolia from Morocco and to evaluate its in vitro antibacterial activity against antibiotic-resistant bacteria isolated from clinical infections. The antimicrobial activity was assessed by a broth micro-well dilution method using multiresistant clinical isolates of 11 pathogenic bacteria: Klebsiella pneumoniae subsp. pneumoniae, Klebsiella ornithinolytica, Escherichia coli, Enterobacter cloacae, Enterobacter aerogenes, Providencia rettgeri, Citrobacter freundii, Hafnia alvei, Salmonella spp., Acinetobacter baumannii and methicillin-resistant Staphylococcus aureus. The main compounds of the oil were carvacrol (48.9%), E-caryophyllene (10.8%) and caryophyllene oxide (7.7%). The oil showed activity against all tested strains with minimal inhibitory concentration (MIC) values ranging between 1% and 4%. For most of the strains, the MIC value was equivalent to the minimal bactericidal concentration value, indicating a clear bactericidal effect of L. coronopifolia EO.
Ella Mendelson - One of the best experts on this subject based on the ideXlab platform.
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isolation of genetically unrelated blandm 1 positive Providencia rettgeri strains in israel
Journal of Clinical Microbiology, 2013Co-Authors: Shiraz Gefenhalevi, Musa Hindiyeh, Debby Bendavid, Gill Smollan, Ohad Galmor, Roberto Azar, Mariana Castanheira, Natasha Belausov, Galia Rahav, Ella MendelsonAbstract:The emergence of the novel carbapenem resistance mechanism New Delhi metallo-β-lactamase ( bla NDM-1) in Enterobacteriaceae has presented a new challenge to health care providers on how to deal with infected or colonized patients. In most cases, the bla NDM-1 gene is borne on large plasmids;
Maria Victoria Ovalleguerro - One of the best experts on this subject based on the ideXlab platform.
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emergencia de Providencia rettgeri ndm 1 en dos departamentos de colombia 2012 2013
Enfermedades Infecciosas Y Microbiologia Clinica, 2017Co-Authors: Sandrayamile Saavedrarojas, Carolina Duartevalderrama, Marianilse Gonzalezdearias, Maria Victoria OvalleguerroAbstract:Resumen Introduccion En Colombia, entre 2012 y 2013 se identificaron 19 aislamientos con NDM, de los cuales 14 correspondian a Providencia rettgeri. Metodos Los aislamientos se identificaron con Vitek-2, y la sensibilidad antimicrobiana se evaluo por microdilucion en caldo. Las carbapenemasas se determinaron fenotipicamente con test de Hodge modificado y pruebas de sinergia con EDTA/SMA y APB, genotipicamente por PCR usando iniciadores especificos para KPC, GES, IMP, VIM, OXA-48 y NDM, y las relaciones geneticas se establecieron con Diversilab. Resultados Los aislamientos fueron resistentes a carbapenemicos, cefalosporinas de tercera generacion, piperacilina-tazobactam, amicacina, gentamicina y tigeciclina, excepto a aztreonam. Todos los aislamientos fueron positivos para EDTA/SMA y NDM-1 y negativos para APB y otras carbapenemasas. Se definieron 2 grupos geneticos: grupo 1 (n = 9 aislamientos), grupo 2 (n = 4 aislamientos), y un aislamiento no relacionado geneticamente. Conclusion Este trabajo describe la circulacion de grupos de P. rettgeri productores de NDM-1 en Colombia.
Tatsuya Tada - One of the best experts on this subject based on the ideXlab platform.
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emergence of carbapenem resistant Providencia rettgeri and Providencia stuartii producing imp type metallo β lactamase in japan
Antimicrobial Agents and Chemotherapy, 2020Co-Authors: Shu Iwata, Tatsuya Tada, Tomomi Hishinuma, Mari Tohya, Satoshi Oshiro, Kyoko Kuwaharaarai, Miho Ogawa, Masahiro Shimojima, Teruo KirikaeAbstract:Four Providencia rettgeri isolates and one Providencia stuartii isolate were obtained from urine samples of five patients in 2018 in Japan. All of the isolates were resistant to imipenem and meropenem, and three were highly resistant to both carbapenems, with MICs of 512 μg/ml. The three highly carbapenem-resistant isolates harbored bla IMP-70, encoding a variant of IMP-1 metallo-β-lactamase with two amino acid substitutions (Val67Phe and Phe87Val), and the other two harbored bla IMP-1 and bla IMP-11, respectively. Whole-genome sequencing revealed that an isolate harbored two copies of bla IMP-1 on the chromosome and that the other four harbored a copy of bla IMP-11 or bla IMP-70 in a plasmid. Expression of bla IMP-70 conferred carbapenem resistance in Escherichia coli Recombinant IMP-70 and an IMP-1 variant with Val67Phe but without Phe87Val had significant higher hydrolytic activities against meropenem than recombinant IMP-1, indicating that an amino acid substitution of Val67Phe affects increased activities against meropenem in IMP-70. These results suggest that Providencia spp. become more highly resistant to carbapenems by acquisition of two copies of bla IMP-1 or by mutation of bla IMP genes with amino acid substitutions, such as bla IMP-70.
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ndm 1 metallo β lactamase and arma 16s rrna methylase producing Providencia rettgeri clinical isolates in nepal
BMC Infectious Diseases, 2014Co-Authors: Tatsuya Tada, Teruo Kirikae, Tohru Miyoshiakiyama, Rajan K Dahal, Hiroshi Ohara, Kayo Shimada, Bharat Mani PokhrelAbstract:Drug-resistant Providencia rettgeri producing metallo-β-lactamase and 16S rRNA methylase has been reported in several countries. We analyzed P. rettgeri clinical isolates with resistance to carbapenems and aminoglycosides in a hospital in Nepal. Five clinical isolates of multidrug-resistant P. rettgeri were obtained in a hospital in Nepal. Antimicrobial susceptibilities were determined using the microdilution method and entire genomes were sequenced to determine drug-resistant genes. Epidemiological analysis was performed by pulsed-field gel electrophoresis. Four of the 5 isolates were resistant to carbapenems (imipenem and meropenem), with MICs ≥16 mg/L, with the remaining isolate showing intermediate resistance to imipenem, with an MIC of 2 mg/L and susceptibility to meropenem with an MIC ≤1 mg/L. All 5 isolates had bla VEB-1. Of the 4 carbapenem-resistant strains, 3 had bla NDM-1 and 1 had bla OXA-72. All isolates were highly resistant to aminoglycosides (MICs ≥1,024 mg/L) and harbored armA. As the result of pulsed-field gel electrophoresis pattern analysis in the 5 P. rettgeri isolates, 4 had identical PFGE patterns and the fifth showed 95.7% similarity. This is the first report describing multidrug-resistant P. rettgeri strains harboring bla NDM-1 or bla OXA-72 and armA isolated from patients in Nepal.
