The Experts below are selected from a list of 216 Experts worldwide ranked by ideXlab platform
Felix Zulhendri - One of the best experts on this subject based on the ideXlab platform.
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optimizing metabolic stress disinfection and disinfestation components to control Pseudococcus longispinus
Journal of Economic Entomology, 2012Co-Authors: Felix Zulhendri, Nihal H De Silva, Lisa E. Jamieson, Siew Young Quek, Conrad O Perera, Allan B. WoolfAbstract:Metabolic stress disinfection and disinfestation (MSDD) has been demonstrated to effectively control longtailed mealybug, Pseudococcus longispinus (Targioni Tozzetti). Standard components previously used for testing MSDD system included a 30-min physical phase of short cycles pressure changes followed by a 60-min chemical phase using ethanol vapor at 10 kPa. This study investigated the effect of varying the following MSDD components on mealybug mortality: duration of the physical and chemical phases, ethanol concentration, and extent of vacuum during the chemical phase. Mealybug mortality responses were analyzed, and the components were optimized using binary logistical regression to achieve 99% mortality of three life stages of the longtailed mealybug (adults, second- and third-instar nymphs and crawlers). Data indicated that the optimal components to achieve 99% mortality of all life stages were a 30-min physical phase and a 45-min chemical phase with 275 mg/liter ethanol at 30 kPa. Optimized components were obtained using binary logistical regression models. These optimized components yielded a 15-min reduction in total treatment time and a 20-kPa decrease in pressure during the chemical phase. Achievement of optimal insecticidal efficacy required all four MSDD components. Nevertheless, optimization and validation achieved 17 and 22% reductions in duration of treatment time and extent of vacuum, respectively.
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the effect of metabolic stress disinfection and disinfestation msdd on hass avocado fruit physiology and mortality of longtailed mealybug Pseudococcus longispinus
Postharvest Biology and Technology, 2012Co-Authors: Felix Zulhendri, Lisa E. Jamieson, R.m. Mcdonald, Siew Young Quek, Conrad O Perera, P G Connolly, Allan B. WoolfAbstract:Abstract Metabolic stress disinfection and disinfestation (MSDD) is a potential quarantine treatment in which a combination of cycles of rapid decompression and compression are followed by exposure to ethanol vapour under decompression. The response of ‘Hass’ avocado ( Persea americana Mill., cv. Hass) to MSDD treatment for control of longtailed mealybug ( Pseudococcus longispinus ) was investigated. The best treatment for the most resistant life stage (2nd/3rd instars) was 90-min MSDD treatment with 371 mg L −1 ethanol. Early and late season ‘Hass’ avocados were subjected to MSDD treatments (with 371 mg L −1 ethanol), or in air (control). Following the treatments, early season fruit were ripened at 20 °C and 25 °C. Half of the late season fruit were ripened at either 20 °C or 25 °C, and the remainder were stored at 5.5 °C for 6 weeks, then ripened at 20 °C. There were no significant difference in quality and rot incidence between non-treated controls and MSDD-treated fruit. The main disorders found were stem-end and body rots, vascular browning and flesh greying for the stored fruit. There were also no significant differences in fruit respiration rate or ethylene production. Thus, MSDD was shown to be a potentially ‘soft’ disinfestation treatment for surface pests of avocado.
Allan B. Woolf - One of the best experts on this subject based on the ideXlab platform.
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optimizing metabolic stress disinfection and disinfestation components to control Pseudococcus longispinus
Journal of Economic Entomology, 2012Co-Authors: Felix Zulhendri, Nihal H De Silva, Lisa E. Jamieson, Siew Young Quek, Conrad O Perera, Allan B. WoolfAbstract:Metabolic stress disinfection and disinfestation (MSDD) has been demonstrated to effectively control longtailed mealybug, Pseudococcus longispinus (Targioni Tozzetti). Standard components previously used for testing MSDD system included a 30-min physical phase of short cycles pressure changes followed by a 60-min chemical phase using ethanol vapor at 10 kPa. This study investigated the effect of varying the following MSDD components on mealybug mortality: duration of the physical and chemical phases, ethanol concentration, and extent of vacuum during the chemical phase. Mealybug mortality responses were analyzed, and the components were optimized using binary logistical regression to achieve 99% mortality of three life stages of the longtailed mealybug (adults, second- and third-instar nymphs and crawlers). Data indicated that the optimal components to achieve 99% mortality of all life stages were a 30-min physical phase and a 45-min chemical phase with 275 mg/liter ethanol at 30 kPa. Optimized components were obtained using binary logistical regression models. These optimized components yielded a 15-min reduction in total treatment time and a 20-kPa decrease in pressure during the chemical phase. Achievement of optimal insecticidal efficacy required all four MSDD components. Nevertheless, optimization and validation achieved 17 and 22% reductions in duration of treatment time and extent of vacuum, respectively.
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the effect of metabolic stress disinfection and disinfestation msdd on hass avocado fruit physiology and mortality of longtailed mealybug Pseudococcus longispinus
Postharvest Biology and Technology, 2012Co-Authors: Felix Zulhendri, Lisa E. Jamieson, R.m. Mcdonald, Siew Young Quek, Conrad O Perera, P G Connolly, Allan B. WoolfAbstract:Abstract Metabolic stress disinfection and disinfestation (MSDD) is a potential quarantine treatment in which a combination of cycles of rapid decompression and compression are followed by exposure to ethanol vapour under decompression. The response of ‘Hass’ avocado ( Persea americana Mill., cv. Hass) to MSDD treatment for control of longtailed mealybug ( Pseudococcus longispinus ) was investigated. The best treatment for the most resistant life stage (2nd/3rd instars) was 90-min MSDD treatment with 371 mg L −1 ethanol. Early and late season ‘Hass’ avocados were subjected to MSDD treatments (with 371 mg L −1 ethanol), or in air (control). Following the treatments, early season fruit were ripened at 20 °C and 25 °C. Half of the late season fruit were ripened at either 20 °C or 25 °C, and the remainder were stored at 5.5 °C for 6 weeks, then ripened at 20 °C. There were no significant difference in quality and rot incidence between non-treated controls and MSDD-treated fruit. The main disorders found were stem-end and body rots, vascular browning and flesh greying for the stored fruit. There were also no significant differences in fruit respiration rate or ethylene production. Thus, MSDD was shown to be a potentially ‘soft’ disinfestation treatment for surface pests of avocado.
J. G. Charles - One of the best experts on this subject based on the ideXlab platform.
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transmission of grapevine leafroll associated closteroviruses by Pseudococcus longispinus and p calceolariae
Plant Pathology, 1997Co-Authors: C. L. Petersen, J. G. CharlesAbstract:The transmission of two closteroviruses associated with grapevine leafroll, GLRaV-1 and GLRaV-3, from grapevine to grapevine by the mealybugs, Pseudococcus longispinus and P. calceolariae (Homoptera: Pseudococcidae) was studied. Controlled transmission experiments using the first and third instars of each insect were conducted twice during the 1993–94 growing season to investigate the consequence of virus accumulation within the donor vine leaf tissue on the incidence of virus transmission to healthy recipient vines. Transmission of GLRaV-1 and GLRaV-3 was determined by ELISA testing recipient vines in July 1994 and March 1995. GLRaV-3 was transmitted to recipient vines by P. longispinus and P. calceolariae first instars only. An increase in virus titre within the season did not significantly alter the transmission rate of GLRaV-3 by either P. longispinus or P. calceolariae first instars. P. longispinus and P. calceolariae failed to transmit GLRaV-1 to recipient vines.
Valdir Atsushi Yuki - One of the best experts on this subject based on the ideXlab platform.
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transmissao experimental do grapevine virus b pela cochonilha Pseudococcus longispinus targioni tozzetti hemiptera pseudococcidae
Summa Phytopathologica, 2006Co-Authors: Hugo Kuniyuki, Cristina Granara De Willink, Ricardo Gioria, Jose Polese Soares Novo, Jorge Alberto Marques Rezende, Valdir Atsushi YukiAbstract:In the State of Sao Paulo, Brazil, there are two isolates of Grapevine virus B (GVB) associated with grapevine corky bark disease (GCB). Although serologically similar, they induce distinct reaction on some grape varieties. They are called GVB-C for common isolate and GVB-I for isolate obtained from the variety Italia. The objective of this work was to verify the transmission of both GVB isolates from infected to healthy plants by the mealybug Pseudococcus longispinus. The transmission of the virus was determined by visual analysis of symptoms, ELISA and RT-PCR. In all transmission experiments, grape indicator plants that had been exposed to presumably viruliferous mealybugs reacted in 8-12 months with a typical symptoms of GCB. Healthy LN-33 plants, maintained around one GCB-C affected LN-33 plant, highly infested by the mealybug, became infected with incidence of 54.2% after four years. Experimental inoculation of healthy LN-33 plants with viruliferous mealybugs resulted in infection rates of 46.2% for GVB-C and 40.0% for GVB-I, after three years. Although P. longispinus occurs eventually in Sao Paulo State vineyards, preventive control measures for this insect must be taken on areas where healthy clones of scion and rootstock varieties are maintained.
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transmissao do grapevine leafroll associated virus 3 pela cochonilha Pseudococcus longispinus targioni tozetti hemiptera pseudococcidae
Summa Phytopathologica, 2005Co-Authors: Hugo Kuniyuki, Cristina Granara De Willink, Jose Polese Soares Novo, Jorge Alberto Marques Rezende, Valdir Atsushi YukiAbstract:This work reports the transmission of the Grapevine leafroll-associated virus 3 (GLRaV-3) from infected to healthy plants by the mealybug Pseudococcus longispinus. Mixed stages of mealybugs were left to feed on the vines infected with GLRaV-3 for an acquisition access time of 10 days. After this period, 10 to 15 insects were transferred to healthy grapevines for an inoculation access period of 7 days, after which plants were sprayed with insecticide. The transference of mealybugs was made by placing leaf pieces of infected vines on healthy test plants. The transmission rate was 64% for the indicator variety LN-33 and 60% for Mission. The transmission of the virus was determined by symptoms and ELISA. Nimphs of the mealybug, fed for ten days on leafroll-infected grapevine in groups of 1, 2, 3, 4, 5, and 10 individuals, showed GLRaV-3 concentrations sufficient for detection by ELISA in all groups, but at different percentages. The species P. longispinus occurs occasionally in Sao Paulo State vineyards, but precautions must be taken on areas where healthy clones of scion and rootstock varieties are maintained.
Michael J.w. Copland - One of the best experts on this subject based on the ideXlab platform.
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defensive behaviour of the longtailed mealybug Pseudococcus longispinus targioni tozzetti hemiptera pseudococcidae against the brown lacewing sympherobius fallax navas neuroptera hemerobiidae
Entomologica, 2016Co-Authors: W A Gillani, Michael J.w. CoplandAbstract:DEFENSIVE BEHAVIOUR OF THE LONGTAILED MEALYBUG Pseudococcus longispinus (TARGIONI TOZZETTI) (HEMIPTERA: PSEUDOCOCCIDAE) AGAINST THE BROWN LACEWING SYMPHEROBIUS FALLAX NAVAS (NEUROPTERA: HEMEROBIIDAE). The defensive tactics of 2nd- and 3rd-instar nymphal and adult Pseudococcus longispinus (Targioni Tozzetti) against larval Sympherobius fallax Navas were studied. When attacked by 1st- and 2nd-instar predators, these three mealybug stages were able to secrete ostiolar fluids which spread and hardened on the mouthparts of the predators; 1st-instar mealybugs appeared to be unable to secrete ostiolar fluids. Adult mealybugs also successfully defended themselves against 1st-instar predators by simply pushing them away with their cerarial wax fringe. Of the lacewing stages, 3rd-instar S. fallax larvae were the most successful at overcoming the defense tactics of P. longispinus. Key words: lacewings, Chrysopidae, Chrysoperla carnea, behavioural defence, morphological defence, potato sprouts, haemolymph.
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studies on the feeding preference of brown lacewing sympherobius fallax navas larvae for different stages of long tailed mealy bug Pseudococcus longispinus targioni and tozzetti
Pakistan Entomologist, 2009Co-Authors: W A Gillani, Michael J.w. Copland, Shazia RajaAbstract:Pseudococcus longispinus (Targioni-Tozzetti) has a wide distribution in tropical and subtropical regions of the world. It attacks many fruit trees, crop plants, vegetables and ornamentals as well as greenhouse plants. Experiments were done to determine the feeding preference of brown lacewing Sympherobius fallax (Navas) larvae of different stages on long-tailed mealy bug Pseudococcus longispinus . It was found that in the no choice experiment the first-instar Sympherobius fallax (Navas) consumed second-stage mealy bug more than any other stages and did not eat fourth (adult) stage while second-stage S. fallax preferred third-stage mealybugs. Thirdstage S. fallax also preferred third-stage mealybugs. In the choice experiment the first-stage larval predators preferred second-stage mealybugs significantly more than the other two stages while the second- and third-stage predators preferred third-stage mealybug significantly more than the second and the fourth stages. It was also determined that darkness has a marked effect on the feeding efficiency of all stages of S. fallax . The number of mealy bugs eaten in the light was significantly greater than in the darkness in all the stages of S. fallax .
