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Manoj Kumar Singh - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of Tobacco leaf curl Pusa virus, a new monopartite Begomovirus associated with tobacco leaf curl disease in India
Virus genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had
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molecular characterization of tobacco leaf curl Pusa virus a new monopartite begomovirus associated with tobacco leaf curl disease in india
Virus Genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had <89% sequence homology with DNA-A of all the known begomoviruses. The isolate is tentatively named as Tobacco leaf curl Pusa virus [India:Pusa:2010]. The betasatellite (HQ180395) associated with TbLCD in Pusa was identified as a variant of Tomato leaf curl Bangladesh betasatellite [IN:Raj:03], with which it shared 90.4% sequence identity. The alphasatellite (HQ180392) associated with the disease had highest 87% nucleotide sequence identity with Tomato leaf curl alphasatellite. The Begomovirus, betasatellite, and alphasatellite associated with TbLCD in Pusa, Bihar, India were found to be recombinants of extant begomoviruses, betasatellites and alphasatellites spreading in the Indian sub-continent and South-East Asia.
Anupam Varma - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of Tobacco leaf curl Pusa virus, a new monopartite Begomovirus associated with tobacco leaf curl disease in India
Virus genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had
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molecular characterization of tobacco leaf curl Pusa virus a new monopartite begomovirus associated with tobacco leaf curl disease in india
Virus Genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had <89% sequence homology with DNA-A of all the known begomoviruses. The isolate is tentatively named as Tobacco leaf curl Pusa virus [India:Pusa:2010]. The betasatellite (HQ180395) associated with TbLCD in Pusa was identified as a variant of Tomato leaf curl Bangladesh betasatellite [IN:Raj:03], with which it shared 90.4% sequence identity. The alphasatellite (HQ180392) associated with the disease had highest 87% nucleotide sequence identity with Tomato leaf curl alphasatellite. The Begomovirus, betasatellite, and alphasatellite associated with TbLCD in Pusa, Bihar, India were found to be recombinants of extant begomoviruses, betasatellites and alphasatellites spreading in the Indian sub-continent and South-East Asia.
Birendra Singh - One of the best experts on this subject based on the ideXlab platform.
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Integrated management of Fusarium wilt by combined soil application and seed dressing formulations of Trichoderma species to increase grain yield of chickpea
International Journal of Pest Management, 2013Co-Authors: Sunil C Dubey, Aradhika Tripathi, Birendra SinghAbstract:Wilt caused by the fungus Fusarium oxysporum f. sp. ciceris adversely affects the productivity of cultivated chickpea. For the management of this disease, seed and soil application formulations developed from another fungus, Trichoderma species, were evaluated. In pot experiments, T. harzianum-based formulations Pusa 5SD for seed dressing and Pusa Biopellet (PBP) 10G and Pusa Biogranule (PBG) 5 for soil application, and T. viride-based formulations Pusa 5SD for seed dressing and PBP 4G and PBG 4 for soil application, were found to be highly effective against the disease. A combination of PBP 4G (T. viride) for soil application and Pusa 5SD (T. harzianum) for seed treatment together with a fungicide, carboxin, provided the highest seed germination, shoot and root lengths and grain yield with the lowest incidence of wilt in chickpea under field conditions. Individually, soil application of PBP 4G, and seed treatment with Pusa 5SD were effective in reducing the incidence of wilt and increasing the grain yiel...
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evaluation of seed dressing and soil application formulations of trichoderma species for integrated management of dry root rot of chickpea
Biocontrol Science and Technology, 2011Co-Authors: Sunil C Dubey, Aradhika Tripathi, R Bhavani, Birendra SinghAbstract:Abstract The efficacy of the newly developed seed dressing and soil application formulations of Trichoderma viride, T. virens and T. harzianum were evaluated individually and in combinations under pot and field experiments for the management of dry root rot (Rhizoctonia bataticola) of chickpea (Cicer arientinum). In pot experiments, T. harzianum based seed dressing formulation, Pusa 5SD, and soil application formulations, Pusa Biogranule 5 (PBG 5) and Pusa Biopellet 10G (PBP 10G), were found to be effective in reducing dry root rot incidence in chickpea and increasing the seed germination, shoot and root lengths of the crop. Under field experiments, a combination of soil application of T. harzianum based PBP 10G and seed treatment with Pusa 5SD+carboxin was found to be the best by providing the highest seed germination, shoot and root lengths and grain yield and the lowest dry root rot incidence in chickpea.
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development of Pusa 5sd for seed dressing and Pusa biopellet 10g for soil application formulations of trichoderma harzianum and their evaluation for integrated management of dry root rot of mungbean vigna radiata
Biological Control, 2009Co-Authors: Sunil C Dubey, R Bhavani, Birendra SinghAbstract:Various seed dressing and soil application formulations were developed from Trichoderma viride, T. virens and T. harzianum to increase the shelf life of bio-formulations used to manage dry root rot (Rhizoctonia bataticola) of mungbean (Vigna radiata), a major yield limiting factor in mungbean production. The shelf life of the formulations developed in the present study was monitored by counting colony forming units (cfu) up to 25 months of storage at room temperature (26 ± 8 °C). A newly developed seed dressing formulation, Pusa 5SD based on peat powder (47.5%), Sabudana powder (Manihot esculenta) (47.5%) and carboxymethyl cellulose (5%) and a newly developed soil application formulation, Pusa Biopellet (PBP) based on sodium alginate, aluminium silicate, Sabudana powder and tap water (1:5:5:100 w/w/w/v) exhibited longer shelf life. Another formulation Pusa Biogranule (PBG) based on wheat and pulse brans varied in cfu counts during different periods of storage. Pusa 5SD could be used up to 25 months of storage while PBP 10G and PBG 5 could be used up to 15 months of storage (>105 cfu). The efficacy of the formulations was evaluated in pot experiments against the disease. In these experiments, T. harzianum based PBP 10G and PBG 5 for soil application, and Pusa 5SD for seed treatment were found to be superior to others in reducing the dry root rot incidence, and increasing the seed germination and shoot and root lengths. However, a combination of soil application of PBP 10G (T. harzianum) and seed treatment with T. harzianum based Pusa 5SD + carboxin was found superior to the use of any of these formulations alone in reducing the dry root rot incidence (87.2%) and increasing the seed germination (43.0%), shoot length (40.3%), root length (37.0%) and grain yield (54.6%) of mungbean crop over those of untreated control under sick field conditions.
K. Singh - One of the best experts on this subject based on the ideXlab platform.
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Molecular characterization of Tobacco leaf curl Pusa virus, a new monopartite Begomovirus associated with tobacco leaf curl disease in India
Virus genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had
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molecular characterization of tobacco leaf curl Pusa virus a new monopartite begomovirus associated with tobacco leaf curl disease in india
Virus Genes, 2011Co-Authors: Manoj Kumar Singh, K. Singh, Q. M. R. Haq, B. Mandal, Anupam VarmaAbstract:Leaf curl disease of tobacco (TbLCD) is endemic in India. A monopartite Begomovirus, a betasatellite and an alphasatellite were found associated with the disease in Pusa, Bihar. The DNA-A of the Begomovirus associated with TbLCD in Pusa, Bihar was found to comprise of 2707 nt with a typical Old World begomovirus-like genome organization. The full-length sequence of DNA-A [HQ180391] showed that the Pusa isolate is a newly described member of the genus Begomovirus, as it had <89% sequence homology with DNA-A of all the known begomoviruses. The isolate is tentatively named as Tobacco leaf curl Pusa virus [India:Pusa:2010]. The betasatellite (HQ180395) associated with TbLCD in Pusa was identified as a variant of Tomato leaf curl Bangladesh betasatellite [IN:Raj:03], with which it shared 90.4% sequence identity. The alphasatellite (HQ180392) associated with the disease had highest 87% nucleotide sequence identity with Tomato leaf curl alphasatellite. The Begomovirus, betasatellite, and alphasatellite associated with TbLCD in Pusa, Bihar, India were found to be recombinants of extant begomoviruses, betasatellites and alphasatellites spreading in the Indian sub-continent and South-East Asia.
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Forty years of papaya research at Pusa, Bihar, India.
Acta Horticulturae, 2010Co-Authors: K. Singh, M. Ram, A. KumarAbstract:Systematic research work on papaya was started during 1966-67 at Indian Agricultural Research Institute Regional Station, Pusa, Bihar, India with an objective to breed the varieties of uniform, high yielding with better quality for wider adaptability. Four varieties viz., Pusa Delicious, Pusa Majesty (Gynodioecious), Pusa Giant and Pusa Dwarf (Dioecious) were developed through continuous sibmating and selection by conventional breeding. Pusa Nanha (Mutant dwarf) was developed through mutation breeding by treating the seeds of papaya strain Pusa 1-15 with 15 Kr gamma rays. Comprehensive studies on sex inheritance in inter and intra-varietal crosses of papaya were attempted and a significant deviation of male and hermaphrodite from the expected ratio was observed. Genetic constitution of sex reversing male in papaya was established. Standardized the production technology of papaya in respect to planting density, time, nutrition and papaya based cropping system under North Bihar conditions. A marked reduction in viral infection was found by transplanting the papaya in month of October with heavy manurial doses. A new fungal disease "bud and fruit stalk rot of papaya" was discovered and standardized its control measure. Seed production of papaya under controlled pollination and isolation were done and found that seed produced under controlled pollination maintains maximum varietal purity with very high cost and less seed where as seed produced in isolation is more with low cost.
Jos T. Puthur - One of the best experts on this subject based on the ideXlab platform.
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Seed Hydropriming Enhances Osmotic Stress Tolerance Potential in Vigna radiata
Agricultural Research, 2018Co-Authors: K. C. Jisha, Jos T. PuthurAbstract:Green gram (Vigna radiata (L.) Wilczek) is a widely cultivated pulse crop in India, and it holds a key importance in food supplement due to its protein-rich edible seeds. This crop usually experiences abiotic stresses like drought and salinity during the cultivation period. The investigation was carried out to study the effect of hydropriming on osmotic stress tolerance potential of three Vigna radiata varieties, with varied abiotic stress tolerance potential. Hydropriming enhances the photosynthetic activities of the seedlings. Moreover, hydropriming resulted in reduction of proline content in Pusa Ratna and Pusa 9531 seedlings, whereas the salt-tolerant variety Pusa Vishal showed increase in proline content, especially under stressed conditions. Osmotic stress caused increase in MDA content in all the varieties studied, and this increase was significantly minimized by the influence of hydropriming. Hydropriming also enhanced the activities of antioxidant enzymes in the seedlings. From the results of this study, it is concluded that hydropriming of seeds improved the drought and salinity stress tolerance potential of all the three green gram varieties and it was significantly evident in the abiotic stress-sensitive variety Pusa Ratna as compared to Pusa 9531 (drought tolerant) and Pusa Vishal (NaCl tolerant).
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halopriming of seeds imparts tolerance to nacl and peg induced stress in vigna radiata l wilczek varieties
Physiology and Molecular Biology of Plants, 2014Co-Authors: K. C. Jisha, Jos T. PuthurAbstract:The investigation was carried out to study the effect of halopriming on NaCl and polyethylene glycol-6000 (PEG-6000) induced stress tolerance potential of three Vigna radiata (L.) Wilczek varieties, with varied abiotic stress tolerance potential. Halopriming is a seed priming technique in which the seeds were soaked in various salt solutions (in this study NaCl was used). The results of the study indicated that the application of stresses (both NaCl and PEG) induced retardation of growth attributes (measured in terms of shoot length, fresh weight, dry weight) and decrease in physiological attributes like total chlorophyll content, metabolites, photosynthetic and mitochondrial activity of the seedlings in all three V. radiata (L.) varieties. However, halopriming of the seeds could reduce the extent of decrease in these biological attributes. NaCl and PEG stress also caused increase in MDA content (a product of membrane lipid peroxidation) in all the varieties studied and this increase was significantly minimized under halopriming. From the present investigation it was evident that among the green gram varieties studied, Pusa Vishal, a NaCl tolerant variety showed enhanced tolerance to NaCl and PEG induced stress, when the seeds were subjected to halopriming followed by Pusa Ratna (stress sensitive variety). Pusa 9531 (drought tolerant variety) also showed positive halopriming effects but it was less significant when compared to other two varieties. It could be concluded that halopriming improved the drought and salinity stress tolerance potential of all varieties and it was significantly higher in the Pusa Vishal as compared to Pusa 9531 and Pusa Ratna.
