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Mohamed N. Rahaman - One of the best experts on this subject based on the ideXlab platform.

  • evaluation of borate bioactive glass scaffolds as a controlled delivery system for copper ions in stimulating osteogenesis and angiogenesis in Bone healing
    Journal of Materials Chemistry B, 2014
    Co-Authors: Hui Wang, Mohamed N. Rahaman, Wenhai Huang, Changqing Zhang, Shichang Zhao, Jie Zhou, Youqu Shen, Deping Wang
    Abstract:

    Biocompatible synthetic scaffolds with enhanced osteogenic and angiogenic capacity are of great interest for the repair of large (critical size) Bone defects. In this study, we investigated an approach based on the controlled delivery of copper (Cu) ions from borate bioactive glass scaffolds for stimulating angiogenesis and osteogenesis in a rodent calvarial defect model. Borate glass scaffolds (pore size = 200–400 μm) doped with varying amounts of Cu (0–3.0 wt% CuO) were created using a polymer foam replication technique. When immersed in simulated body fluid (SBF) in vitro, the scaffolds released Cu ions into the medium at a rate that was dependent on the amount of Cu in the glass and simultaneously converted to hydroxyapatite (HA). At the concentrations used, the Cu in the glass was not cytotoxic to human Bone marrow derived stem cells (hBMSCs) cultured on the scaffolds and the alkaline phosphatase activity of the hBMSCs increased with increasing Cu in the glass. When implanted in rat calvarial defects for 8 weeks, the scaffolds doped with 3 wt% CuO showed a significantly better capacity to stimulate angiogenesis and Regenerate Bone when compared to the undoped glass scaffolds. Together, these results indicate that the controlled delivery of Cu ions from borate bioactive glass implants is a promising approach in healing Bone defects.

  • a novel injectable borate bioactive glass cement for local delivery of vancomycin to cure osteomyelitis and Regenerate Bone
    Journal of Materials Science: Materials in Medicine, 2014
    Co-Authors: Xu Cui, Cunju Zhao, Hui Wang, Wenhai Huang, Nai Zhou, Deping Wang, Yi Zhu, Shihua Luo, Changqing Zhang, Mohamed N. Rahaman
    Abstract:

    Osteomyelitis (Bone infection) is often difficult to cure. The commonly-used treatment of surgical debridement to remove the infected Bone combined with prolonged systemic and local antibiotic treatment has limitations. In the present study, an injectable borate bioactive glass cement was developed as a carrier for the antibiotic vancomycin, characterized in vitro, and evaluated for its capacity to cure osteomyelitis in a rabbit tibial model. The cement (initial setting time = 5.8 ± 0.6 min; compressive strength = 25.6 ± 0.3 MPa) released vancomycin over ~25 days in phosphate-buffered saline, during which time the borate glass converted to hydroxyapatite (HA). When implanted in rabbit tibial defects infected with methicillin-resistant Staphylococcus aureus (MRSA)-induced osteomyelitis, the vancomycin-loaded cement converted to HA and supported new Bone formation in the defects within 8 weeks. Osteomyelitis was cured in 87 % of the defects implanted with the vancomycin-loaded borate glass cement, compared to 71 % for the defects implanted with vancomycin-loaded calcium sulfate cement. The injectable borate bioactive glass cement developed in this study is a promising treatment for curing osteomyelitis and for regenerating Bone in the defects following cure of the infection.

  • effect of bioactive borate glass microstructure on Bone regeneration angiogenesis and hydroxyapatite conversion in a rat calvarial defect model
    Acta Biomaterialia, 2013
    Co-Authors: Mohamed N. Rahaman, Xin Liu, Delbert E Day, Zackary Brown, Christopher Samujh, Ali Mohammadkhah, Vladimir Dusevich, David J Eick, Lynda F. Bonewald
    Abstract:

    Abstract Borate bioactive glasses are biocompatible and enhance new Bone formation, but the effect of their microstructure on Bone regeneration has received little attention. In this study scaffolds of borate bioactive glass (1393B3) with three different microstructures (trabecular, fibrous, and oriented) were compared for their capacity to Regenerate Bone in a rat calvarial defect model. 12 weeks post-implantation the amount of new Bone, mineralization, and blood vessel area in the scaffolds were evaluated using histomorphometric analysis and scanning electron microscopy. The amount of new Bone formed was 33%, 23%, and 15%, respectively, of the total defect area for the trabecular, oriented, and fibrous microstructures. In comparison, the percent new Bone formed in implants composed of silicate 45S5 bioactive glass particles (250–300 μm) was 19%. Doping the borate glass with copper (0.4 wt.% CuO) had little effect on Bone regeneration in the trabecular and oriented scaffolds, but significantly enhanced Bone regeneration in the fibrous scaffolds (from 15 to 33%). The scaffolds were completely converted to hydroxyapatite within the 12 week implantation. The amount of hydroxyapatite formed, 22%, 35%, and 48%, respectively, for the trabecular, oriented, and fibrous scaffolds, increased with increasing volume fraction of glass in the as-fabricated scaffold. Blood vessels infiltrated into all the scaffolds, but the trabecular scaffolds had a higher average blood vessel area compared with the oriented and fibrous scaffolds. While all three scaffold microstructures were effective in supporting Bone regeneration, the trabecular scaffolds supported more Bone formation and may be more promising in Bone repair.

  • Enhanced Bone regeneration in rat calvarial defects implanted with surface-modified and BMP-loaded bioactive glass (13-93) scaffolds.
    Acta biomaterialia, 2013
    Co-Authors: Xin Liu, Mohamed N. Rahaman, Yongxing Liu, B. Sonny Bal, Lynda F. Bonewald
    Abstract:

    The repair of large Bone defects, such as segmental defects in the long Bones of the limbs, is a challenging clinical problem. Our recent work has shown the ability to create porous scaffolds of silicate 13-93 bioactive glass by robocasting which have compressive strengths comparable to human cortical Bone. The objective of this study was to evaluate the capacity of those strong porous scaffolds with a grid-like microstructure (porosity=50%; filament width=330μm; pore width=300μm) to Regenerate Bone in a rat calvarial defect model. Six weeks post-implantation, the amount of new Bone formed within the implants was evaluated using histomorphometric analysis. The amount of new Bone formed in implants composed of the as-fabricated scaffolds was 32% of the available pore space (area). Pretreating the as-fabricated scaffolds in an aqueous phosphate solution for 1, 3 and 6days to convert a surface layer to hydroxyapatite prior to implantation enhanced new Bone formation to 46%, 57% and 45%, respectively. New Bone formation in scaffolds pretreated for 1, 3 and 6days and loaded with Bone morphogenetic protein-2 (BMP-2) (1μg per defect) was 65%, 61% and 64%, respectively. The results show that converting a surface layer of the glass to hydroxyapatite or loading the surface-treated scaffolds with BMP-2 can significantly improve the capacity of 13-93 bioactive glass scaffolds to Regenerate Bone in an osseous defect. Based on their mechanical properties evaluated previously and their capacity to Regenerate Bone found in this study, these 13-93 bioactive glass scaffolds, pretreated or loaded with BMP-2, are promising in structural Bone repair.

  • Bone regeneration in strong porous bioactive glass 13 93 scaffolds with an oriented microstructure implanted in rat calvarial defects
    Acta Biomaterialia, 2013
    Co-Authors: Xin Liu, Mohamed N. Rahaman
    Abstract:

    There is a need for synthetic Bone graft substitutes to repair large Bone defects resulting from trauma, malignancy and congenital diseases. Bioactive glass has attractive properties as a scaffold material but factors that influence its ability to Regenerate Bone in vivo are not well understood. In the present work, the ability of strong porous scaffolds of 13-93 bioactive glass with an oriented microstructure to Regenerate Bone was evaluated in vivo using a rat calvarial defect model. Scaffolds with an oriented microstructure of columnar pores (porosity=50%; pore diameter=50-150 μm) showed mostly osteoconductive Bone regeneration, and new Bone formation, normalized to the available pore area (volume) of the scaffolds, increased from 37% at 12 weeks to 55% at 24 weeks. Scaffolds of the same glass with a trabecular microstructure (porosity=80%; pore width=100-500 μm), used as the positive control, showed Bone regeneration in the pores of 25% and 46% at 12 and 24 weeks, respectively. The brittle mechanical response of the as-fabricated scaffolds changed markedly to an elastoplastic response in vivo at both implantation times. These results indicate that both groups of 13-93 bioactive glass scaffolds could potentially be used to repair large Bone defects, but scaffolds with the oriented microstructure could also be considered for the repair of loaded Bone.

Sudha Kadiyala - One of the best experts on this subject based on the ideXlab platform.

  • allogeneic mesenchymal stem cells Regenerate Bone in a critical sized canine segmental defect
    Journal of Bone and Joint Surgery American Volume, 2003
    Co-Authors: Treena Livingston Arinzeh, Susan Peter, Michael P Archambault, Christian Van Den Bos, Steve Gordon, Karl H Kraus, Alan Smith, Sudha Kadiyala
    Abstract:

    Background: Mesenchymal stem cells from adult Bone marrow are multipotent cells capable of forming Bone, cartilage, and other connective tissues. In a previous study, we demonstrated that autologous mesenchymal stem cells could repair a critical-sized Bone defect in the dog. The objective of this study was to determine whether the use of allogeneic mesenchymal stem cells could heal a critical-sized Bone defect in the femoral diaphysis in dogs without the use of immunosuppressive therapy. Methods: A critical-sized segmental Bone defect, 21 mm in length, was created in the mid-portion of the femoral diaphysis of twelve adult dogs that weighed between 22 and 25 kg. Each defect was treated with allogeneic mesenchymal stem cells loaded onto a hollow ceramic cylinder consisting of hydroxyapatite-tricalcium phosphate. A complete mismatch between donor stem cells and recipient dogs was identified by dog leukocyte antigen typing prior to implantation. The healing response was evaluated histologically and radiographically at four, eight, and sixteen weeks after implantation. The radiographic and histological results at sixteen weeks were compared with the historical data for the control defects, which included defects that had been treated with a cylinder loaded with autologous mesenchymal stem cells, defects treated with a cylinder without mesenchymal stem cells, and defects that had been left untreated (empty). The systemic immune response was evaluated by the analysis of recipient serum for production of antibodies against allogeneic cells. Results: For defects treated with allogeneic mesenchymal stem cell implants, no adverse host response could be detected at any time-point. Histologically, no lymphocytic infiltration occurred and no antibodies against allogeneic cells were detected. Histologically, by eight weeks, a callus spanned the length of the defect, and lamellar Bone filled the pores of the implant at the host Bone-implant interface. Fluorescently labeled allogeneic cells were also detected. At sixteen weeks, new Bone had formed throughout the implant. These results were consistent with those seen in implants loaded with autologous cells. Implants loaded with allogeneic or autologous stem cells had significantly greater amounts of Bone within the available pore space than did cell-free implants at sixteen weeks (p < 0.05). Conclusions: The results of this study demonstrated that allogeneic mesenchymal stem cells loaded on hydroxyapatite-tricalcium phosphate implants enhanced the repair of a critical-sized segmental defect in the canine femur without the use of immunosuppressive therapy. No adverse immune response was detected in this model. Clinical Relevance: The use of allogeneic mesenchymal stem cells for the repair of large defects may be an alternative to autologous and allogeneic Bone-grafting procedures. An allogeneic approach would enable mesenchymal stem cells to be isolated from any donor, expanded, and cryopreserved, providing a readily available source of cells for Bone tissue engineering.

  • investigation of allogeneic mesenchymal stem cell based alveolar Bone formation preliminary findings
    Clinical Oral Implants Research, 2003
    Co-Authors: Ingeborg J De Kok, Christian Van Den Bos, Sudha Kadiyala, Susan J Peter, Michael Archambault, Lkramuddin Aukhil, Lyndon F Cooper
    Abstract:

    This study was designed to evaluate mesenchymal stem cell (MSC)-based alveolar Bone regeneration in a canine alveolar saddle defect model. MSCs were loaded onto hydroxyapatite/tricalcium phosphate (HA/TCP) matrices. Scanning electron microscopic (SEM) evaluation demonstrated greater than 75% MSC coverage of the HA/TCP porous surface prior to placement regardless of MSC donor. Matrices, 6 mm x 6 mm x 20 mm, with and without cells, were implanted for 4 and 9 weeks, then removed for histological evaluation of Bone formation. Cell-free control matrices were compared with MSC-loaded matrices post implantation. Histomorphometrical analysis showed that equivalent amounts of new Bone were formed within the pores of the matrices loaded with autologous MSCs or MSCs from an unrelated donor. Bone formation in the cell-free HA/TCP matrices was less extensive. There was no histologic evidence of an immunological response to autologous MSCs. Surprisingly, allogeneic MSC implantation also failed to provoke an immune response. Analysis of circulating antibody levels against MSCs supported the hypothesis that neither autologous nor allogeneic MSCs induced a systemic response by the host. Analysis of dye-labelled MSCs in histological sections confirmed that the MSCs persisted in the implants throughout the course of the experiment. At 9 weeks, labelled cells were present within the lacunae of newly formed Bone. We conclude that autologous and allogeneic MSCs have the capacity to Regenerate Bone within craniofacial defects.

  • Bone regeneration by implantation of purified culture expanded human mesenchymal stem cells
    Journal of Orthopaedic Research, 1998
    Co-Authors: Scott P Bruder, A A Kurth, Marie Shea, Wilson C Hayes, Neelam Jaiswal, Sudha Kadiyala
    Abstract:

    Bone marrow contains a population of rare progenitor cells capable of differentiating into Bone, cartilage, tendon, and other connective tissues. These cells, referred to as mesenchymal stem cells, can be purified and culture-expanded from animals and humans and have been shown to Regenerate functional tissue when delivered to the site of musculoskeletal defects in experimental animals. To test the ability of purified human mesenchymal stem cells to heal a clinically significant Bone defect, mesenchymal stem cells isolated from normal human Bone marrow were culture-expanded, loaded onto a ceramic carrier, and implanted into critical-sized segmental defects in the femurs of adult athymic rats. For comparison, cell-free ceramics were implanted in the contralateral limb. The animals were euthanized at 4, 8, or 12 weeks, and healing Bone defects were compared by high-resolution radiography, immunohistochemistry, quantitative histomorphometry, and biomechanical testing. In mesenchymal stem cell-loaded samples, radiographic and histologic evidence of new Bone was apparent by 8 weeks and histomorphometry demonstrated increasing Bone formation through 12 weeks. Biomechanical evaluation confirmed that femurs implanted with mesenchymal stem cell-loaded ceramics were significantly stronger than those that received cell-free ceramics. These studies demonstrate that human mesenchymal stem cells can Regenerate Bone in a clinically significant osseous defect and may therefore provide an alternative to autogenous Bone grafts.

Xin Liu - One of the best experts on this subject based on the ideXlab platform.

  • effect of bioactive borate glass microstructure on Bone regeneration angiogenesis and hydroxyapatite conversion in a rat calvarial defect model
    Acta Biomaterialia, 2013
    Co-Authors: Mohamed N. Rahaman, Xin Liu, Delbert E Day, Zackary Brown, Christopher Samujh, Ali Mohammadkhah, Vladimir Dusevich, David J Eick, Lynda F. Bonewald
    Abstract:

    Abstract Borate bioactive glasses are biocompatible and enhance new Bone formation, but the effect of their microstructure on Bone regeneration has received little attention. In this study scaffolds of borate bioactive glass (1393B3) with three different microstructures (trabecular, fibrous, and oriented) were compared for their capacity to Regenerate Bone in a rat calvarial defect model. 12 weeks post-implantation the amount of new Bone, mineralization, and blood vessel area in the scaffolds were evaluated using histomorphometric analysis and scanning electron microscopy. The amount of new Bone formed was 33%, 23%, and 15%, respectively, of the total defect area for the trabecular, oriented, and fibrous microstructures. In comparison, the percent new Bone formed in implants composed of silicate 45S5 bioactive glass particles (250–300 μm) was 19%. Doping the borate glass with copper (0.4 wt.% CuO) had little effect on Bone regeneration in the trabecular and oriented scaffolds, but significantly enhanced Bone regeneration in the fibrous scaffolds (from 15 to 33%). The scaffolds were completely converted to hydroxyapatite within the 12 week implantation. The amount of hydroxyapatite formed, 22%, 35%, and 48%, respectively, for the trabecular, oriented, and fibrous scaffolds, increased with increasing volume fraction of glass in the as-fabricated scaffold. Blood vessels infiltrated into all the scaffolds, but the trabecular scaffolds had a higher average blood vessel area compared with the oriented and fibrous scaffolds. While all three scaffold microstructures were effective in supporting Bone regeneration, the trabecular scaffolds supported more Bone formation and may be more promising in Bone repair.

  • Enhanced Bone regeneration in rat calvarial defects implanted with surface-modified and BMP-loaded bioactive glass (13-93) scaffolds.
    Acta biomaterialia, 2013
    Co-Authors: Xin Liu, Mohamed N. Rahaman, Yongxing Liu, B. Sonny Bal, Lynda F. Bonewald
    Abstract:

    The repair of large Bone defects, such as segmental defects in the long Bones of the limbs, is a challenging clinical problem. Our recent work has shown the ability to create porous scaffolds of silicate 13-93 bioactive glass by robocasting which have compressive strengths comparable to human cortical Bone. The objective of this study was to evaluate the capacity of those strong porous scaffolds with a grid-like microstructure (porosity=50%; filament width=330μm; pore width=300μm) to Regenerate Bone in a rat calvarial defect model. Six weeks post-implantation, the amount of new Bone formed within the implants was evaluated using histomorphometric analysis. The amount of new Bone formed in implants composed of the as-fabricated scaffolds was 32% of the available pore space (area). Pretreating the as-fabricated scaffolds in an aqueous phosphate solution for 1, 3 and 6days to convert a surface layer to hydroxyapatite prior to implantation enhanced new Bone formation to 46%, 57% and 45%, respectively. New Bone formation in scaffolds pretreated for 1, 3 and 6days and loaded with Bone morphogenetic protein-2 (BMP-2) (1μg per defect) was 65%, 61% and 64%, respectively. The results show that converting a surface layer of the glass to hydroxyapatite or loading the surface-treated scaffolds with BMP-2 can significantly improve the capacity of 13-93 bioactive glass scaffolds to Regenerate Bone in an osseous defect. Based on their mechanical properties evaluated previously and their capacity to Regenerate Bone found in this study, these 13-93 bioactive glass scaffolds, pretreated or loaded with BMP-2, are promising in structural Bone repair.

  • Bone regeneration in strong porous bioactive glass 13 93 scaffolds with an oriented microstructure implanted in rat calvarial defects
    Acta Biomaterialia, 2013
    Co-Authors: Xin Liu, Mohamed N. Rahaman
    Abstract:

    There is a need for synthetic Bone graft substitutes to repair large Bone defects resulting from trauma, malignancy and congenital diseases. Bioactive glass has attractive properties as a scaffold material but factors that influence its ability to Regenerate Bone in vivo are not well understood. In the present work, the ability of strong porous scaffolds of 13-93 bioactive glass with an oriented microstructure to Regenerate Bone was evaluated in vivo using a rat calvarial defect model. Scaffolds with an oriented microstructure of columnar pores (porosity=50%; pore diameter=50-150 μm) showed mostly osteoconductive Bone regeneration, and new Bone formation, normalized to the available pore area (volume) of the scaffolds, increased from 37% at 12 weeks to 55% at 24 weeks. Scaffolds of the same glass with a trabecular microstructure (porosity=80%; pore width=100-500 μm), used as the positive control, showed Bone regeneration in the pores of 25% and 46% at 12 and 24 weeks, respectively. The brittle mechanical response of the as-fabricated scaffolds changed markedly to an elastoplastic response in vivo at both implantation times. These results indicate that both groups of 13-93 bioactive glass scaffolds could potentially be used to repair large Bone defects, but scaffolds with the oriented microstructure could also be considered for the repair of loaded Bone.

Lynda F. Bonewald - One of the best experts on this subject based on the ideXlab platform.

  • effect of bioactive borate glass microstructure on Bone regeneration angiogenesis and hydroxyapatite conversion in a rat calvarial defect model
    Acta Biomaterialia, 2013
    Co-Authors: Mohamed N. Rahaman, Xin Liu, Delbert E Day, Zackary Brown, Christopher Samujh, Ali Mohammadkhah, Vladimir Dusevich, David J Eick, Lynda F. Bonewald
    Abstract:

    Abstract Borate bioactive glasses are biocompatible and enhance new Bone formation, but the effect of their microstructure on Bone regeneration has received little attention. In this study scaffolds of borate bioactive glass (1393B3) with three different microstructures (trabecular, fibrous, and oriented) were compared for their capacity to Regenerate Bone in a rat calvarial defect model. 12 weeks post-implantation the amount of new Bone, mineralization, and blood vessel area in the scaffolds were evaluated using histomorphometric analysis and scanning electron microscopy. The amount of new Bone formed was 33%, 23%, and 15%, respectively, of the total defect area for the trabecular, oriented, and fibrous microstructures. In comparison, the percent new Bone formed in implants composed of silicate 45S5 bioactive glass particles (250–300 μm) was 19%. Doping the borate glass with copper (0.4 wt.% CuO) had little effect on Bone regeneration in the trabecular and oriented scaffolds, but significantly enhanced Bone regeneration in the fibrous scaffolds (from 15 to 33%). The scaffolds were completely converted to hydroxyapatite within the 12 week implantation. The amount of hydroxyapatite formed, 22%, 35%, and 48%, respectively, for the trabecular, oriented, and fibrous scaffolds, increased with increasing volume fraction of glass in the as-fabricated scaffold. Blood vessels infiltrated into all the scaffolds, but the trabecular scaffolds had a higher average blood vessel area compared with the oriented and fibrous scaffolds. While all three scaffold microstructures were effective in supporting Bone regeneration, the trabecular scaffolds supported more Bone formation and may be more promising in Bone repair.

  • Enhanced Bone regeneration in rat calvarial defects implanted with surface-modified and BMP-loaded bioactive glass (13-93) scaffolds.
    Acta biomaterialia, 2013
    Co-Authors: Xin Liu, Mohamed N. Rahaman, Yongxing Liu, B. Sonny Bal, Lynda F. Bonewald
    Abstract:

    The repair of large Bone defects, such as segmental defects in the long Bones of the limbs, is a challenging clinical problem. Our recent work has shown the ability to create porous scaffolds of silicate 13-93 bioactive glass by robocasting which have compressive strengths comparable to human cortical Bone. The objective of this study was to evaluate the capacity of those strong porous scaffolds with a grid-like microstructure (porosity=50%; filament width=330μm; pore width=300μm) to Regenerate Bone in a rat calvarial defect model. Six weeks post-implantation, the amount of new Bone formed within the implants was evaluated using histomorphometric analysis. The amount of new Bone formed in implants composed of the as-fabricated scaffolds was 32% of the available pore space (area). Pretreating the as-fabricated scaffolds in an aqueous phosphate solution for 1, 3 and 6days to convert a surface layer to hydroxyapatite prior to implantation enhanced new Bone formation to 46%, 57% and 45%, respectively. New Bone formation in scaffolds pretreated for 1, 3 and 6days and loaded with Bone morphogenetic protein-2 (BMP-2) (1μg per defect) was 65%, 61% and 64%, respectively. The results show that converting a surface layer of the glass to hydroxyapatite or loading the surface-treated scaffolds with BMP-2 can significantly improve the capacity of 13-93 bioactive glass scaffolds to Regenerate Bone in an osseous defect. Based on their mechanical properties evaluated previously and their capacity to Regenerate Bone found in this study, these 13-93 bioactive glass scaffolds, pretreated or loaded with BMP-2, are promising in structural Bone repair.

Biju Parekkadan - One of the best experts on this subject based on the ideXlab platform.

  • orthogonal potency analysis of mesenchymal stromal cell function during ex vivo expansion
    Experimental Cell Research, 2018
    Co-Authors: Danika Khong, Amy Singleton, Ling Yee Chin, Shilpaa Mukundan, Biju Parekkadan
    Abstract:

    Abstract Adult Bone marrow mesenchymal stromal cells (MSCs) have cross-functional, intrinsic potency that is of therapeutic interest. Their ability to Regenerate Bone, fat, and cartilage, modulate the immune system, and nurture the growth and function of other Bone marrow hematopoietic stem/progenitor cells have all been evaluated by transplant applications of MSCs. These applications require the isolation and expansion scaled cell production. To investigate biophysical properties of MSCs that can be feasibly utilized as predictors of bioactivity during biomanufacturing, we used a low-density seeding model to drive MSCs into proliferative stress and exhibit the hallmark characteristics of in vitro aging. A low-density seeding method was used to generate MSCs from passages 1–7 to simulate serial expansion of these cells to maximize yield from a single donor. MSCs were subjected to three bioactivity assays in parallel to ascertain whether patterns in MSC age, size, and shape were associated with the outcomes of the potency assays. MSC age was found to be a predictor of adipogenesis, while cell and nuclear shape was strongly associated to hematopoietic-supportive potency. Together, these data evaluate morphological changes associated with cell potency and highlight new strategies for purification or alternatives to assessing MSC quality.