The Experts below are selected from a list of 44934 Experts worldwide ranked by ideXlab platform

Stephen F. Badylak - One of the best experts on this subject based on the ideXlab platform.

  • Host Response to Implanted Materials and Devices: An Overview
    The Immune Response to Implanted Materials and Devices, 2016
    Co-Authors: Michelle E. Scarritt, Ricardo Londono, Stephen F. Badylak
    Abstract:

    The host Response to implanted materials and devices is influenced not only by the design of the material itself, but also by the local and systemic environment of the host. Much of the early Response follows the well-described cascade of events of wound healing from hemostasis to scar formation. An implanted material can positively or negatively modulate this cascade of events, culminating in a constructive Remodeling Response, a persistent inflammatory Response, a foreign body Response with encapsulation, or an adaptive immune Response. An overview of these events, as well as the influence of biologic versus synthetic materials, is discussed in this chapter.

  • the effect of terminal sterilization on the material properties and in vivo Remodeling of a porcine dermal biologic scaffold
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Christopher A Carruthers, Luai Huleihel, Christian A Ranallo, Elizabeth W Kollar, Christopher L. Dearth, Stephen F. Badylak
    Abstract:

    Abstract Biologic scaffolds composed of extracellular matrix are commonly used in a variety of surgical procedures. The Food and Drug Administration typically regulates biologic scaffolds as medical devices, thus requiring terminal sterilization prior to clinical use. However, to date, no consensus exists for the most effective yet minimally destructive sterilization protocol for biologic scaffold materials. The objective of the present study was to characterize the effect of ethylene oxide, gamma irradiation and electron beam (e-beam) irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. Outcome measures included biochemical, structural, and mechanical properties as well as cytocompatibility in vitro. In vivo evaluation utilized a rodent model to examine the host Response to the materials following 7, 14, and 35 days. The host Response to each experimental group was determined by quantitative histologic methods and by immunolabeling for macrophage polarization (M1/M2). In vitro results show that increasing irradiation dosage resulted in a dose dependent decrease in mechanical properties compared to untreated controls. Ethylene oxide-treated porcine dermal ECM resulted in decreased DNA content, extractable total protein, and bFGF content compared to untreated controls. All ETO treated, gamma irradiated, and e-beam irradiated samples had similar cytocompatibility scores in vitro. However, in vivo results showed that increasing dosages of e-beam and gamma irradiation elicited an increased rate of degradation of the biologic scaffold material following 35 days. Statement of Significance The FDA typically regulates biologic scaffolds derived from mammalian tissues as medical devices, thus requiring terminal sterilization prior to clinical use. However, there is little data and no consensus for the most effective yet minimally destructive sterilization protocol for such materials. The present study characterized the effect of common sterilization methods: ethylene oxide, gamma irradiation and electron beam irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. The results of the study will aid in the meaningful selection of sterilization methods for biologic scaffold materials.

  • inhibition of cox1 2 alters the host Response and reduces ecm scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Ricardo Londono, Christopher L. Dearth, Peter F Slivka, Scott A Stewart, Francis X Pizza, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Inhibition of COX1/2 alters the host Response and reduces ECM scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury.
    Acta biomaterialia, 2015
    Co-Authors: Christopher L. Dearth, Timothy J Keane, Ricardo Londono, Peter F Slivka, Scott A Stewart, Francis X Pizza, Justin K. Tay, Qingnian Goh, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Decellularization of mammalian tissues: Preparing extracellular matrix bioscaffolds
    Characterisation and Design of Tissue Scaffolds, 2015
    Co-Authors: Timothy J Keane, Lindsey T. Saldin, Stephen F. Badylak
    Abstract:

    Abstract Biologic surgical mesh materials composed of mammalian extracellular matrix (ECM) have been successfully used to alter the host default Response to injury, and induce functional, site-appropriate tissue regeneration. The choice of decellularization agents, techniques, and post-processing terminal sterilization steps will depend on the intended application, tissue type, and decellularization criteria. An overview of biological, chemical, and physical decellularization methods are presented, with a description of their mechanism and effect upon the ECM. The objective is to achieve cellular removal and sterility while best preserving native ECM structure and function. This balance of factors will ultimately determine the host Remodeling Response to the material.

Christopher L. Dearth - One of the best experts on this subject based on the ideXlab platform.

  • the effect of terminal sterilization on the material properties and in vivo Remodeling of a porcine dermal biologic scaffold
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Christopher A Carruthers, Luai Huleihel, Christian A Ranallo, Elizabeth W Kollar, Christopher L. Dearth, Stephen F. Badylak
    Abstract:

    Abstract Biologic scaffolds composed of extracellular matrix are commonly used in a variety of surgical procedures. The Food and Drug Administration typically regulates biologic scaffolds as medical devices, thus requiring terminal sterilization prior to clinical use. However, to date, no consensus exists for the most effective yet minimally destructive sterilization protocol for biologic scaffold materials. The objective of the present study was to characterize the effect of ethylene oxide, gamma irradiation and electron beam (e-beam) irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. Outcome measures included biochemical, structural, and mechanical properties as well as cytocompatibility in vitro. In vivo evaluation utilized a rodent model to examine the host Response to the materials following 7, 14, and 35 days. The host Response to each experimental group was determined by quantitative histologic methods and by immunolabeling for macrophage polarization (M1/M2). In vitro results show that increasing irradiation dosage resulted in a dose dependent decrease in mechanical properties compared to untreated controls. Ethylene oxide-treated porcine dermal ECM resulted in decreased DNA content, extractable total protein, and bFGF content compared to untreated controls. All ETO treated, gamma irradiated, and e-beam irradiated samples had similar cytocompatibility scores in vitro. However, in vivo results showed that increasing dosages of e-beam and gamma irradiation elicited an increased rate of degradation of the biologic scaffold material following 35 days. Statement of Significance The FDA typically regulates biologic scaffolds derived from mammalian tissues as medical devices, thus requiring terminal sterilization prior to clinical use. However, there is little data and no consensus for the most effective yet minimally destructive sterilization protocol for such materials. The present study characterized the effect of common sterilization methods: ethylene oxide, gamma irradiation and electron beam irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. The results of the study will aid in the meaningful selection of sterilization methods for biologic scaffold materials.

  • inhibition of cox1 2 alters the host Response and reduces ecm scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Ricardo Londono, Christopher L. Dearth, Peter F Slivka, Scott A Stewart, Francis X Pizza, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Inhibition of COX1/2 alters the host Response and reduces ECM scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury.
    Acta biomaterialia, 2015
    Co-Authors: Christopher L. Dearth, Timothy J Keane, Ricardo Londono, Peter F Slivka, Scott A Stewart, Francis X Pizza, Justin K. Tay, Qingnian Goh, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Preparation and Characterization of a Biologic Scaffold from Esophageal Mucosa
    Biomaterials, 2013
    Co-Authors: Timothy J Keane, Janet Reing, Christopher A Carruthers, Ricardo Londono, Christopher L. Dearth, Christopher J Medberry, Ryan M. Carey, Antonio D'amore, Stephen F. Badylak
    Abstract:

    Biologic scaffolds composed of extracellular matrix (ECM) are commonly used to facilitate a constructive Remodeling Response in several types of tissue, including the esophagus. Surgical manipulation of the esophagus is often complicated by stricture, but preclinical and clinical studies have shown that the use of an ECM scaffold can mitigate stricture and promote a constructive outcome after resection of full circumference esophageal mucosa. Recognizing the potential benefits of ECM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo Remodeling properties of ECM from porcine esophageal mucosa. The developed protocol for esophageal ECM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a basement membrane complex. Perivascular stem cells remained viable when seeded upon the esophageal ECM scaffold in-vitro, and the in-vivo host Response showed a pattern of constructive Remodeling when implanted in soft tissue.

Timothy J Keane - One of the best experts on this subject based on the ideXlab platform.

  • the effect of terminal sterilization on the material properties and in vivo Remodeling of a porcine dermal biologic scaffold
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Christopher A Carruthers, Luai Huleihel, Christian A Ranallo, Elizabeth W Kollar, Christopher L. Dearth, Stephen F. Badylak
    Abstract:

    Abstract Biologic scaffolds composed of extracellular matrix are commonly used in a variety of surgical procedures. The Food and Drug Administration typically regulates biologic scaffolds as medical devices, thus requiring terminal sterilization prior to clinical use. However, to date, no consensus exists for the most effective yet minimally destructive sterilization protocol for biologic scaffold materials. The objective of the present study was to characterize the effect of ethylene oxide, gamma irradiation and electron beam (e-beam) irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. Outcome measures included biochemical, structural, and mechanical properties as well as cytocompatibility in vitro. In vivo evaluation utilized a rodent model to examine the host Response to the materials following 7, 14, and 35 days. The host Response to each experimental group was determined by quantitative histologic methods and by immunolabeling for macrophage polarization (M1/M2). In vitro results show that increasing irradiation dosage resulted in a dose dependent decrease in mechanical properties compared to untreated controls. Ethylene oxide-treated porcine dermal ECM resulted in decreased DNA content, extractable total protein, and bFGF content compared to untreated controls. All ETO treated, gamma irradiated, and e-beam irradiated samples had similar cytocompatibility scores in vitro. However, in vivo results showed that increasing dosages of e-beam and gamma irradiation elicited an increased rate of degradation of the biologic scaffold material following 35 days. Statement of Significance The FDA typically regulates biologic scaffolds derived from mammalian tissues as medical devices, thus requiring terminal sterilization prior to clinical use. However, there is little data and no consensus for the most effective yet minimally destructive sterilization protocol for such materials. The present study characterized the effect of common sterilization methods: ethylene oxide, gamma irradiation and electron beam irradiation on the material properties and the elicited in vivo Remodeling Response of a porcine dermal biologic scaffold. The results of the study will aid in the meaningful selection of sterilization methods for biologic scaffold materials.

  • inhibition of cox1 2 alters the host Response and reduces ecm scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Ricardo Londono, Christopher L. Dearth, Peter F Slivka, Scott A Stewart, Francis X Pizza, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Inhibition of COX1/2 alters the host Response and reduces ECM scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury.
    Acta biomaterialia, 2015
    Co-Authors: Christopher L. Dearth, Timothy J Keane, Ricardo Londono, Peter F Slivka, Scott A Stewart, Francis X Pizza, Justin K. Tay, Qingnian Goh, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Decellularization of mammalian tissues: Preparing extracellular matrix bioscaffolds
    Characterisation and Design of Tissue Scaffolds, 2015
    Co-Authors: Timothy J Keane, Lindsey T. Saldin, Stephen F. Badylak
    Abstract:

    Abstract Biologic surgical mesh materials composed of mammalian extracellular matrix (ECM) have been successfully used to alter the host default Response to injury, and induce functional, site-appropriate tissue regeneration. The choice of decellularization agents, techniques, and post-processing terminal sterilization steps will depend on the intended application, tissue type, and decellularization criteria. An overview of biological, chemical, and physical decellularization methods are presented, with a description of their mechanism and effect upon the ECM. The objective is to achieve cellular removal and sterility while best preserving native ECM structure and function. This balance of factors will ultimately determine the host Remodeling Response to the material.

  • Decellularization of mammalian tissues: Preparing extracellular matrix bioscaffolds
    Characterisation and Design of Tissue Scaffolds, 2015
    Co-Authors: Timothy J Keane, Lindsey T. Saldin
    Abstract:

    Abstract Biologic surgical mesh materials composed of mammalian extracellular matrix (ECM) have been successfully used to alter the host default Response to injury, and induce functional, site-appropriate tissue regeneration. The choice of decellularization agents, techniques, and post-processing terminal sterilization steps will depend on the intended application, tissue type, and decellularization criteria. An overview of biological, chemical, and physical decellularization methods are presented, with a description of their mechanism and effect upon the ECM. The objective is to achieve cellular removal and sterility while best preserving native ECM structure and function. This balance of factors will ultimately determine the host Remodeling Response to the material.

Ricardo Londono - One of the best experts on this subject based on the ideXlab platform.

  • Host Response to Implanted Materials and Devices: An Overview
    The Immune Response to Implanted Materials and Devices, 2016
    Co-Authors: Michelle E. Scarritt, Ricardo Londono, Stephen F. Badylak
    Abstract:

    The host Response to implanted materials and devices is influenced not only by the design of the material itself, but also by the local and systemic environment of the host. Much of the early Response follows the well-described cascade of events of wound healing from hemostasis to scar formation. An implanted material can positively or negatively modulate this cascade of events, culminating in a constructive Remodeling Response, a persistent inflammatory Response, a foreign body Response with encapsulation, or an adaptive immune Response. An overview of these events, as well as the influence of biologic versus synthetic materials, is discussed in this chapter.

  • inhibition of cox1 2 alters the host Response and reduces ecm scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury
    Acta Biomaterialia, 2016
    Co-Authors: Timothy J Keane, Ricardo Londono, Christopher L. Dearth, Peter F Slivka, Scott A Stewart, Francis X Pizza, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Inhibition of COX1/2 alters the host Response and reduces ECM scaffold mediated constructive tissue Remodeling in a rodent model of skeletal muscle injury.
    Acta biomaterialia, 2015
    Co-Authors: Christopher L. Dearth, Timothy J Keane, Ricardo Londono, Peter F Slivka, Scott A Stewart, Francis X Pizza, Justin K. Tay, Qingnian Goh, Stephen F. Badylak
    Abstract:

    Abstract Extracellular matrix (ECM) has been used as a biologic scaffold material to both reinforce the surgical repair of soft tissue and serve as an inductive template to promote a constructive tissue Remodeling Response. Success of such an approach is dependent on macrophage-mediated degradation and Remodeling of the biologic scaffold. Macrophage phenotype during these processes is a predictive factor of the eventual Remodeling outcome. ECM scaffolds have been shown to promote an anti-inflammatory or M2-like macrophage phenotype in vitro that includes secretion of downstream products of cycolooxygenases 1 and 2 (COX1/2). The present study investigated the effect of a common COX1/2 inhibitor (Aspirin) on macrophage phenotype and tissue Remodeling in a rodent model of ECM scaffold treated skeletal muscle injury. Inhibition of COX1/2 reduced the constructive Remodeling Response by hindering myogenesis and collagen deposition in the defect area. The inhibited Response was correlated with a reduction in M2-like macrophages in the defect area. The effects of Aspirin on macrophage phenotype were corroborated using an established in vitro macrophage model which showed a reduction in both ECM induced prostaglandin secretion and expression of a marker of M2-like macrophages (CD206). These results raise questions regarding the common peri-surgical administration of COX1/2 inhibitors when biologic scaffold materials are used to facilitate muscle repair/regeneration. Statement of significance COX1/2 inhibitors such as nonsteroidal anti-inflammatory drugs (NSAIDs) are routinely administered post-surgically for analgesic purposes. While COX1/2 inhibitors are important in pain management, they have also been shown to delay or diminish the healing process, which calls to question their clinical use for treating musculotendinous injuries. The present study aimed to investigate the influence of a common NSAID, Aspirin, on the constructive Remodeling Response mediated by an ECM scaffold (UBM) in a rat skeletal muscle injury model. The COX1/2 inhibitor, Aspirin, was found to mitigate the ECM scaffold-mediated constructive Remodeling Response both in an in vitro co-culture system and an in vivo rat model of skeletal muscle injury. The results presented herein provide data showing that NSAIDs may significantly alter tissue Remodeling outcomes when a biomaterial is used in a regenerative medicine/tissue engineering application. Thus, the decision to prescribe NSAIDs to manage the symptoms of inflammation post-ECM scaffold implantation should be carefully considered.

  • Preparation and Characterization of a Biologic Scaffold from Esophageal Mucosa
    Biomaterials, 2013
    Co-Authors: Timothy J Keane, Janet Reing, Christopher A Carruthers, Ricardo Londono, Christopher L. Dearth, Christopher J Medberry, Ryan M. Carey, Antonio D'amore, Stephen F. Badylak
    Abstract:

    Biologic scaffolds composed of extracellular matrix (ECM) are commonly used to facilitate a constructive Remodeling Response in several types of tissue, including the esophagus. Surgical manipulation of the esophagus is often complicated by stricture, but preclinical and clinical studies have shown that the use of an ECM scaffold can mitigate stricture and promote a constructive outcome after resection of full circumference esophageal mucosa. Recognizing the potential benefits of ECM derived from homologous tissue (i.e., site-specific ECM), the objective of the present study was to prepare, characterize, and assess the in-vivo Remodeling properties of ECM from porcine esophageal mucosa. The developed protocol for esophageal ECM preparation is compliant with previously established criteria of decellularization and results in a scaffold that maintains important biologic components and an ultrastructure consistent with a basement membrane complex. Perivascular stem cells remained viable when seeded upon the esophageal ECM scaffold in-vitro, and the in-vivo host Response showed a pattern of constructive Remodeling when implanted in soft tissue.

Wilfried Mullens - One of the best experts on this subject based on the ideXlab platform.