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Elżbieta Fugas - One of the best experts on this subject based on the ideXlab platform.
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
Halina Ekiert - One of the best experts on this subject based on the ideXlab platform.
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Coumarins and alkaloids in shoot culture of Ruta graveolens L.
Acta Societatis Botanicorum Poloniae, 2014Co-Authors: Halina Ekiert, Wanda KisielAbstract:A shoot culture of Ruta graveolens L. ( Rutaceae ) was maintained in the stationary liquid phase. From the cultured shoots seven compounds were isolated and identified as psoralen, bergapten, xanthotoxin, isopimpinellin (linear furanocoumarins), Rutamarin (linear dihydrofuranocoumarin), kokusaginine and skimmianine (furanoquinoline alkaloids) by spectral methods. The compounds are known as secondary metabolites of the intact plant, as well as its cell and tissue cultures.
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
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Arbutin production in Ruta graveolens L. and Hypericum perforatum L. in vitro cultures
Acta Physiologiae Plantarum, 2010Co-Authors: Agata Piekoszewska, Halina Ekiert, Szymon ZubekAbstract:Optimization of conditions for hydroquinone biotransformation into its β- d -glucoside, arbutin, in agitated shoot cultures of Ruta graveolens L. and Hypericum perforatum L. allowed us to obtain a maximum content of this important therapeutic and cosmetic product of 7.8 and 7.2% (dry weight), respectively. These contents are higher than respective values required for standardization of known arbutin-containing plant raw materials according to the European Pharmacopoeia and national pharmacopoeias of European countries.
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Accumulation of biologically active furanocoumarins in agitated cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams.
Die Pharmazie, 2005Co-Authors: Halina EkiertAbstract:This study was designed to investigate the dynamics of accumulation of linear furanocoumarins (psoralen, bergapten, xanthotoxin, isopimpinellin, imperatorin) and their biogenetic precursor, umbelliferone, in agitated cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams during 6-week growth cycles. The metabolites under study were almost exclusively accumulated in the cultured biomass. The total content of all metabolites increased 4.8- and 2.0-fold, in R. graveolens and R. graveolens ssp. divaricata cultures, respectively. Xanthotoxin and bergapten, which are the most important therapeutic compounds, were the dominating metabolites in cultures of both plants. The maximum content of xanthotoxin (25.0 mg/100g dry wt.) and bergapten (18.4mg/100g dry wt) and the maximum content of all metabolites (64.0 mg/100 g dry wt) in R. graveolens ssp. divaricata callus obtained on the 35 t h culture day were relatively low. However, maximum contents of xanthotoxin (136.8 mg/100 g dry wt), bergapten (210.4 mg/100 g dry wt.) and isopimpinellin (96.7 mg/100 g dry wt), and total content of all metabolites in R. graveolens shoots (520.8 mg/100 g dry wt) obtained on the 42 n d culture day are interesting from a practical point of view.
Agnieszka Szopa - One of the best experts on this subject based on the ideXlab platform.
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
Agnieszka Szewczyk - One of the best experts on this subject based on the ideXlab platform.
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
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Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions
Plant Cell Tissue and Organ Culture (PCTOC), 2012Co-Authors: Agnieszka Szopa, Halina Ekiert, Agnieszka Szewczyk, Elżbieta FugasAbstract:Extracts from the biomass of Ruta graveolens and Ruta graveolens ssp. divaricata cultured in vitro under different light conditions (far-red, red and blue light, UV-A irradiation, in darkness and white light) were tested for the amounts of free phenolic acids and cinnamic acid (twelve compounds) as well as furanocoumarins and umbelliferone (seven compounds) using HPLC methods. Total amounts of the investigated groups of compounds in the cultures of both plants increased from 2.6 to 6.7 times, depending on light quality, and the maximum values reached were 106.50 and 1,276.74 mg 100 g^−1 DW (in R. graveolens ), and 106.97 and 262.54 mg 100 g^−1 DW (in the subspecies), respectively. Both white light and blue light were equally beneficial for the total production of phenolic acids in cultures of both plants, whereas the total production of furanocoumarins was clearly better stimulated by blue light in R. graveolens and by darkness in the subspecies (i.e. the amounts were respectively 1.44 and 1.7 times higher than in the biomass cultivated under white light). The amounts of individual compounds in both plant cultures increased from about 2.2 to 26.3 times depending on light quality. The following bioactive compounds were obtained in quantities which are of interest from a practical perspective: in R. graveolens culture—protocatechuic acid (45 mg 100 g^−1 DW), isopimpinellin (about 500 mg 100 g^−1 DW) and bergapten (about 270 mg 100 g^−1 DW), and in the subspecies culture: p -coumaric acid (70 mg 100 g^−1 DW) and isopimpinellin (about 210 mg 100 g^−1 DW).
A. Helen - One of the best experts on this subject based on the ideXlab platform.
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Oral administration of alkaloid fraction from Ruta graveolens inhibits oxidative stress and inflammation in hypercholesterolemic rabbits
Pharmaceutical Biology, 2013Co-Authors: M. Ratheesh, A. HelenAbstract:AbstractContext: The anti-atherogenic effect of alkaloid fraction from Ruta graveolens Linn (Rutaceae) extract is suspected to be related to its activities of antioxidation and anti-inflammation.Objective: This study investigated the efficacy of alkaloid fraction isolated from Ruta graveolens (AFR) in reducing oxidative damage and inflammation in hypercholesteremic rabbits.Materials and methods: The New Zealand white rabbits were randomly divided into three groups: Group I rabbits were fed with normal chow diet for 90 d. Group II rabbits were fed with 1% cholesterol-enriched diet. Group III rabbits were fed with 1% cholesterol-enriched diet together with AFR (10 mg/kg/daily for 90 d).Results and discussion: The results showed that on treatment with AFR significantly lowered the level of total cholesterol and LDL-C and showed an increment in the level of HDL-C. LD50 of the AFR in rats is greater than 525 mg/kg. Activities of antioxidant enzymes such as superoxide dismutase, catalase and glutathione peroxid...
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Anti-inflammatory effect of quinoline alkaloid skimmianine isolated from Ruta graveolens L.
Inflammation Research, 2013Co-Authors: M. Ratheesh, G. Sindhu, A. HelenAbstract:Objective The present study evaluates the anti-inflammatory effect of the quinoline alkaloid skimmianine (SKM), isolated from Ruta graveolens L., against carrageenan-induced acute inflammation.
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Protective Effects of Isolated Polyphenolic and Alkaloid Fractions of Ruta graveolens L. on Acute and Chronic Models of Inflammation
Inflammation, 2010Co-Authors: M. Ratheesh, G. Sindhu, G. L. Shyni, A. HelenAbstract:Ruta graveolens L. (Rutaceae) are traditionally used for the treatment of rheumatism, arthritis and other inflammatory conditions in the traditional medicine of India, were evaluated for their protective effect in acute and chronic models of inflammation. Carrageenan induced rat paw edema and adjuvant induced arthritis were employed as the experimental models of acute and chronic inflammation respectively. Isolated polyphenolic and alkaloid fraction ( AFR) from Ruta graveolens and evaluated its anti inflammatory activity in carrageenan induced acute model. AFR with a dose 10 mg/kg showed higher anti inflammatory effect than polyphenols and standard drug diclofenec. AFR significantly decreased the paw edema in arthritic rats. TBARS, COX-2, 5-LOX and MPO level were decreased and the levels of antioxidant enzymes and GSH level were increased on treatment with AFR . The increment in CRP level and ceruloplasmin level observed in arthritic animals were also found to be significantly restored in AFR treated rats. The results demonstrated the potential beneficiary effect of isolated polyphenolic and alkaloid fraction of Ruta graveolens L. on acute and chronic models of inflammation in rats.
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Methanolic extract of Ruta graveolens L. inhibits inflammation and oxidative stress in adjuvant induced model of arthritis in rats.
Inflammopharmacology, 2009Co-Authors: M. Ratheesh, G. L. Shyni, A. HelenAbstract:Ruta graveolens L. (Rutaceae) are traditionally used for the treatment of rheumatism, arthritis and other inflammatory conditions in the traditional medicine of India. The purpose of this study was to investigate anti-inflammatory and anti-oxidant effects of methanolic extract of Ruta graveolens L. in adjuvant induced arthritis in rats. Methanolic extract of Ruta graveolens (MER) exhibited maximum percentage of oedema inhibition at a dose of 20 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug indomethacin. The activities of cycloxygenase-2 and myeloperoxidase and concentration of thiobarbituric acid reactive substance (TBARS) were decreased and the activities of antioxidant enzymes, vitamins C & E and reduced glutathione level were increased on treatment with MER. The increment in ESR and total WBC, reduction in RBC count and haemoglobin and aberrant changes to the C-reactive protein (CRP) and ceruloplasmin levels observed in the arthritic animals were also found to be significantly restored in MER treated rats. Histopathology of paw tissue showed decreased oedema formation and cellular infiltration on supplementation with MER. Thus the results demonstrated the potential beneficiary effect of methanolic extract of Ruta graveolens on adjuvant induced arthritis in rats.
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Research Article Methanolic extract of Ruta graveolens L. inhibits inflammation and oxidative stress in adjuvant induced model of arthritis in rats
2009Co-Authors: M. Ratheesh, G. L. Shyni, A. HelenAbstract:Ruta graveolens L. (Rutaceae) are traditionally used for the treatment of rheumatism, arthritis and other in- flammatory conditions in the traditional medicine of India. The purpose of this study was to investigate anti-inflamma - tory and anti-oxidant effects of methanolic extract of Ruta graveolens L. in adjuvant induced arthritis in rats. Methanolic extract of Ruta graveolens (MER) exhibited maximum per- centage of oedema inhibition at a dose of 20 mg/kg on 21st day of adjuvant arthritis. The effect was higher than that of standard drug indomethacin. The activities of cycloxygen- ase-2 and myeloperoxidase and concentration of thiobarbitu- ric acid reactive substance (TBARS) were decreased and the activities of antioxidant enzymes, vitamins C & E and reduced glutathione level were increased on treatment with MER. The increment in ESR and total WBC, reduction in RBC count and haemoglobin and aberrant changes to the C-reactive pro- tein (CRP) and ceruloplasmin levels observed in the arthritic animals were also found to be significantly restored in MER treated rats. Histopathology of paw tissue showed decreased oedema formation and cellular infiltration on supplementation with MER. Thus the results demonstrated the potential benefi - ciary effect of methanolic extract of Ruta graveolens on adju- vant induced arthritis in rats.
