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Roy French - One of the best experts on this subject based on the ideXlab platform.
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses
2013Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74�2–76�2 % (nucleotide) and 79�2–81�0 % (amino acid
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Tritimovirus P1 functions as a suppressor of RNA silencing and an enhancer of disease symptoms.
Virus research, 2011Co-Authors: Brock A. Young, Satyanarayana Tatineni, Drake C Stenger, T. Jack Morris, Roy FrenchAbstract:Wheat streak mosaic virus (WSMV) is an eriophyid mite-transmitted virus of the genus Tritimovirus, family Potyviridae. Complete deletion of helper component-proteinase (HC-Pro) has no effect on WSMV virulence or disease synergism, suggesting that a different viral protein suppresses RNA silencing. RNA silencing suppression assays using Nicotiana benthamiana 16C plants expressing GFP were conducted with each WSMV protein; only P1 suppressed RNA silencing. Accumulation of GFP siRNAs was markedly reduced in leaves infiltrated with WSMV P1 at both 3 and 6 days post infiltration relative to WSMV HC-Pro and the empty vector control. On the other hand, helper component-proteinase (HC-Pro) of two species in the mite-transmitted genus Rymovirus, family Potyviridae was demonstrated to be a suppressor of RNA silencing. Symptom enhancement assays were conducted by inoculating Potato virus X (PVX) onto transgenic N. benthamiana. Symptoms produced by PVX were more severe on transgenic plants expressing WSMV P1 or potyvirus HC-Pro compared to transgenic plants expressing GFP or WSMV HC-Pro.
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triticum mosaic virus a distinct member of the family potyviridae with an unusually long leader sequence
Phytopathology, 2009Co-Authors: Satyanarayana Tatineni, Stephen N. Wegulo, Amy D Ziems, Roy FrenchAbstract:Tatineni, S., Ziems, A. D., Wegulo, S. N., and French, R. 2009. Triticum mosaic virus: A distinct member of the family Potyviridae with an unusually long leader sequence. Phytopathology 99:943-950. The complete genome sequence of Triticum mosaic virus (TriMV), a member in the family Potyviridae, has been determined to be 10,266 nucleotides (nt) excluding the 3′ polyadenylated tail. The genome encodes a large polyprotein of 3,112 amino acids with the “hall-mark proteins” of potyviruses, including a small overlapping gene, PIPO, in the P3 cistron. The genome of TriMV has an unusually long 5′ nontranslated region of 739 nt with 12 translation initiation codons and three small open reading frames, which resemble those of the internal ribosome entry site containing 5′ leader sequences of the members of Picornaviridae. Pairwise comparison of 10 putative mature proteins of TriMV with those of representative members of genera in the family Potyviridae revealed 33 to 44% amino acid identity within the highly conserved NIb protein sequence and 15 to 29% amino acid identity within the least conserved P1 protein, suggesting that TriMV is a distinct member in the family Potyviridae. In contrast, TriMV displayed 47 to 65% amino acid sequence identity with available sequences of mature proteins of Sugarcane streak mosaic virus (SCSMV), an unassigned member of the Potyviridae. Phylogenetic analyses of the complete polyprotein, NIa-Pro, NIb, and coat protein sequences of representative species of six genera and unassigned members of the family Potyviridae suggested that TriMV and SCSMV are sister taxa and share a most recent common ancestor with tritimoviruses or ipomoviruses. These results suggest that TriMV and SCSMV should be classified in a new genus, and we propose the genus Poacevirus in the family Potyviridae, with TriMV as the type member. Additional keyword: wheat. Viruses from several different families infect wheat (Triticum aestivum L.) in the Great Plains and other parts of the United States. These viruses include Agropyron mosaic virus (AgMV), Barley yellow dwarf virus, Soil-borne wheat mosaic virus, Triticum mosaic virus (TriMV), Wheat American striate mosaic virus, Wheat mosaic virus, and Wheat streak mosaic virus (WSMV) (6,20,21). Among these viruses, WSMV is an economically important virus causing significant yield losses in the United States (6). TriMV was recently reported from Kansas, naturally infecting WSMV-resistant wheat cultivars (20); however, the impact of this virus on yield losses in wheat remains to be known. The Potyviridae is the largest family of positive-stranded RNA viruses infecting plants, divided into six genera based on their genetic relatedness, vector transmission, and genome organization (2,5). The genus Potyvirus, with Potato virus Y (PVY) as the type member, contains numerous economically important aphidtransmitted virus species and is the most thoroughly characterized genus among the family Potyviridae. Other genera include Rymovirus, with Ryegrass mosaic virus (RGMV) as the type species, transmitted by Abacarus mites; Tritimovirus, with WSMV as the type member, vectored by wheat curl mites (Aceria tosichella); Ipomovirus, with Sweet potato mild mottle virus (SPMMV) as the type species, transmitted by whiteflies; and Macluravirus, with Maclura mosaic virus (MacMV) as the type species, with characteristic short virus particles transmitted by aphids. These five genera all contain monopartite viruses, whereas the genus Bymovirus, with Barley yellow mosaic virus (BaYMV) as the type member, contains bipartite viruses transmitted by plasmodio
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses.
The Journal of general virology, 2002Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74.2-76.2% (nucleotide) and 79.2-81.0% (amino acid) identity. Thus, ONMV should be removed from the genus Rymovirus and designated a definitive member of the genus Tritimovirus. Phylogenetic analyses further suggest that Sugarcane streak mosaic virus is not a tritimovirus, and may represent a new genus within the family Potyviridae.
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molecular cloning sequencing and phylogenetic relationships of a new potyvirus sugarcane streak mosaic virus and a reevaluation of the classification of the potyviridae
Molecular Phylogenetics and Evolution, 1998Co-Authors: Jeffrey S Hall, Roy French, Byron J Adams, Thomas J Parsons, Leslie C Lane, Stanley G JensenAbstract:Abstract The nucleic acid of a serologically distinct potyvirus, originally isolated out of sugar cane from Pakistan, was reverse transcribed and the 3′ terminal 2000 bp was PCR amplified, cloned, and sequenced. Phylogenetic comparisons of viruses representing each genus of the Potyviridae show that the Pakistani isolate is most closely related to the Rymoviruses wheat streak mosaic virus (WSMV) and brome streak mosaic virus. We therefore propose that this new virus species be named sugar cane streak mosaic virus to reflect its similarity to WSMV. The phylogenetic data also show that the genus Rymovirus contains at least two unique evolutionary lineages. Thus the current taxonomy, based on transmission vector, is paraphyletic. We present an analysis of the taxonomic relationships among members of the family and propose a classification that both resolves the paraphyly and more accurately represents the evolutionary history of the Potyviridae.
Drake C Stenger - One of the best experts on this subject based on the ideXlab platform.
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ICTV Virus Taxonomy Profile: Potyviridae.
The Journal of general virology, 2017Co-Authors: Stephen J. Wylie, Frank Rabenstein, Drake C Stenger, Michael J. Adams, Jan Kreuze, Celia Chalam, Juan José López-moya, Kazusato Ohshima, Shelly Praveen, Aiming WangAbstract:The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide. There are eight genera, distinguished by the host range, genomic features and phylogeny of the member viruses. Genomes range from 8.2 to 11.3 kb, with an average size of 9.7 kb. Most genomes are monopartite but those of members of the genus Bymovirus are bipartite. Some members cause serious disease epidemics in cultivated plants. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Potyviridae, which is available at www.ictv.global/report/potyviridae.
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses
2013Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74�2–76�2 % (nucleotide) and 79�2–81�0 % (amino acid
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Tritimovirus P1 functions as a suppressor of RNA silencing and an enhancer of disease symptoms.
Virus research, 2011Co-Authors: Brock A. Young, Satyanarayana Tatineni, Drake C Stenger, T. Jack Morris, Roy FrenchAbstract:Wheat streak mosaic virus (WSMV) is an eriophyid mite-transmitted virus of the genus Tritimovirus, family Potyviridae. Complete deletion of helper component-proteinase (HC-Pro) has no effect on WSMV virulence or disease synergism, suggesting that a different viral protein suppresses RNA silencing. RNA silencing suppression assays using Nicotiana benthamiana 16C plants expressing GFP were conducted with each WSMV protein; only P1 suppressed RNA silencing. Accumulation of GFP siRNAs was markedly reduced in leaves infiltrated with WSMV P1 at both 3 and 6 days post infiltration relative to WSMV HC-Pro and the empty vector control. On the other hand, helper component-proteinase (HC-Pro) of two species in the mite-transmitted genus Rymovirus, family Potyviridae was demonstrated to be a suppressor of RNA silencing. Symptom enhancement assays were conducted by inoculating Potato virus X (PVX) onto transgenic N. benthamiana. Symptoms produced by PVX were more severe on transgenic plants expressing WSMV P1 or potyvirus HC-Pro compared to transgenic plants expressing GFP or WSMV HC-Pro.
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genome sequences of agropyron mosaic virus and hordeum mosaic virus support reciprocal monophyly of the genera potyvirus and Rymovirus in the family potyviridae
Archives of Virology, 2005Co-Authors: Roy C French, Drake C StengerAbstract:Assignment of mite-transmitted species to the genus Rymovirus (family Potyviridae) has changed several times, and the status of the genus has been questioned. To address this issue, complete genome sequences of the Rymoviruses Agropyron mosaic virus (AgMV) and Hordeum mosaic virus (HoMV) were determined. AgMV (9540 nucleotides) and HoMV (9463 nucleotides) each encode a single polyprotein with proteinase cleavage sites demarcating protein products characteristic of monopartite species of the family Potyviridae. Of the described species of Potyviridae, AgMV and HoMV are most closely related to each other (68.5% nucleotide and 71.6% amino acid sequence identity) and equidistant (about 53% nucleotide and about 49% amino acid sequence identity) from a third Rymovirus, Ryegrass mosaic virus (RGMV). Phylogenetic analyses by neighbor joining, maximum parsimony, and Bayesian inference each grouped the three Rymovirus species in an exclusive clade distinct from a clade containing 34 species of the genus Potyvirus. Because AgMV, HoMV, and RGMV share a reciprocal monophyletic relationship with species of the genus Potyvirus and are divergent in sequence and type of vector, the genus Rymovirus should be retained as a taxonomic unit within the family Potyviridae.
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses.
The Journal of general virology, 2002Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74.2-76.2% (nucleotide) and 79.2-81.0% (amino acid) identity. Thus, ONMV should be removed from the genus Rymovirus and designated a definitive member of the genus Tritimovirus. Phylogenetic analyses further suggest that Sugarcane streak mosaic virus is not a tritimovirus, and may represent a new genus within the family Potyviridae.
Frank Rabenstein - One of the best experts on this subject based on the ideXlab platform.
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Plant virome reconstruction and antiviral RNAi characterization by deep sequencing of small RNAs from dried leaves.
Scientific reports, 2019Co-Authors: Victor Golyaev, Thierry Candresse, Frank Rabenstein, Mikhail M PoogginAbstract:In plants, RNA interference (RNAi) generates small interfering (si)RNAs from entire genomes of viruses, satellites and viroids. Therefore, deep small (s)RNA sequencing is a universal approach for virome reconstruction and RNAi characterization. We tested this approach on dried barley leaves from field surveys. Illumina sequencing of sRNAs from 2 plant samples identified in both plants Hordeum vulgare endornavirus (HvEV) and barley yellow mosaic bymovirus (BaYMV) and, additionally in one plant, a novel strain of Japanese soil-borne wheat mosaic furovirus (JSBWMV). De novo and reference-based sRNA assembly yielded complete or near-complete genomic RNAs of these viruses. While plant sRNAs showed broad size distribution, viral sRNAs were predominantly 21 and 22 nucleotides long with 5'-terminal uridine or adenine, and were derived from both genomic strands. These bona fide siRNAs are presumably processed from double-stranded RNA precursors by Dicer-like (DCL) 4 and DCL2, respectively, and associated with Argonaute 1 and 2 proteins. For BaYMV (but not HvEV, or JSBWMV), 24-nucleotide sRNAs represented the third most abundant class, suggesting DCL3 contribution to anti-bymovirus defence. Thus, viral siRNAs are well preserved in dried leaf tissues and not contaminated by non-RNAi degradation products, enabling both complete virome reconstruction and inference of RNAi components mediating antiviral defense.
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ICTV Virus Taxonomy Profile: Potyviridae.
The Journal of general virology, 2017Co-Authors: Stephen J. Wylie, Frank Rabenstein, Drake C Stenger, Michael J. Adams, Jan Kreuze, Celia Chalam, Juan José López-moya, Kazusato Ohshima, Shelly Praveen, Aiming WangAbstract:The Potyviridae is the largest family of RNA plant viruses, members of which have single-stranded, positive-sense RNA genomes and flexuous filamentous particles 680–900 nm long and 11–20 nm wide. There are eight genera, distinguished by the host range, genomic features and phylogeny of the member viruses. Genomes range from 8.2 to 11.3 kb, with an average size of 9.7 kb. Most genomes are monopartite but those of members of the genus Bymovirus are bipartite. Some members cause serious disease epidemics in cultivated plants. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the taxonomy of the Potyviridae, which is available at www.ictv.global/report/potyviridae.
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Main winter barley production areas in France, sampling sites used in the present study.
2017Co-Authors: Mathieu Rolland, Frank Rabenstein, Julie Villemot, Armelle Marais, Sébastien Theil, Chantal Faure, Valérie Cadot, Romain Valade, Cindy Vitry, Thierry CandresseAbstract:Barley production areas are colour-coded according to barley acreage in each department: below 12500ha, white; from 12500 to 25000ha, pale grey; above 25000ha, dark grey (data: Agreste 2014). Each collection site of the survey is represented, and the differential-host experimental sites are shown as black stars. The Bymovirus diversity from these 11 sites is indicated as follows: BaYMV-1 as a black dot, BaYMV-2 as a white dot, BaMMV as a black square and rym5-breaking BaMMV as a white square.
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses
2013Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74�2–76�2 % (nucleotide) and 79�2–81�0 % (amino acid
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Phylogenetic relationships, strain diversity and biogeography of tritimoviruses.
The Journal of general virology, 2002Co-Authors: Frank Rabenstein, Dallas L Seifers, Jörg Schubert, Roy French, Drake C StengerAbstract:North American and Eurasian isolates of Wheat streak mosaic virus (WSMV; genus Tritimovirus) and Oat necrotic mottle virus (ONMV; genus Rymovirus) were examined. Nine WSMV isolates differentially infected oat, barley, inbred maize line SDp2 and sorghum line KS56. The WSMV isolates clustered into groups based on phylogenetic analyses of the capsid protein (CP) cistron and flanking regions. WSMV isolates from the United States (US) and Turkey were closely related, suggesting recent movement between continents. Although more divergent, WSMV from Iran (WSMV-I) also shared a most recent common ancestor with the US and Turkish isolates. Another group of WSMV isolates from central Europe and Russia may represent a distinct Eurasian population. Complete genome sequences of WSMV from the Czech Republic (WSMV-CZ) and Turkey (WSMV-TK1) were determined and comparisons based on complete sequences yielded relationships similar to those based on partial sequences. ONMV-Pp recovered from blue grass (Poa pratensis L.) in Germany displayed the same narrow host range as ONMV-Type from Canada. Western blots revealed a heterologous relationship among CP of WSMV and ONMV. Phylogenetic analyses of the capsid protein cistron and flanking genomic regions indicated that WSMV and ONMV are related species sharing 74.2-76.2% (nucleotide) and 79.2-81.0% (amino acid) identity. Thus, ONMV should be removed from the genus Rymovirus and designated a definitive member of the genus Tritimovirus. Phylogenetic analyses further suggest that Sugarcane streak mosaic virus is not a tritimovirus, and may represent a new genus within the family Potyviridae.
Jeffrey S Hall - One of the best experts on this subject based on the ideXlab platform.
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molecular cloning sequencing and phylogenetic relationships of a new potyvirus sugarcane streak mosaic virus and a reevaluation of the classification of the potyviridae
Molecular Phylogenetics and Evolution, 1998Co-Authors: Jeffrey S Hall, Roy French, Byron J Adams, Thomas J Parsons, Leslie C Lane, Stanley G JensenAbstract:Abstract The nucleic acid of a serologically distinct potyvirus, originally isolated out of sugar cane from Pakistan, was reverse transcribed and the 3′ terminal 2000 bp was PCR amplified, cloned, and sequenced. Phylogenetic comparisons of viruses representing each genus of the Potyviridae show that the Pakistani isolate is most closely related to the Rymoviruses wheat streak mosaic virus (WSMV) and brome streak mosaic virus. We therefore propose that this new virus species be named sugar cane streak mosaic virus to reflect its similarity to WSMV. The phylogenetic data also show that the genus Rymovirus contains at least two unique evolutionary lineages. Thus the current taxonomy, based on transmission vector, is paraphyletic. We present an analysis of the taxonomic relationships among members of the family and propose a classification that both resolves the paraphyly and more accurately represents the evolutionary history of the Potyviridae.
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phylogenetic relationships within the family potyviridae wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus
Phytopathology, 1998Co-Authors: Drake C Stenger, Jeffrey S Hall, Ilryong Choi, Roy FrenchAbstract:Stenger, D. C., Hall, J. S., Choi, I.-R., and French, R. 1998. Phylogenetic relationships within the family Potyviridae: Wheat streak mosaic virus and brome streak mosaic virus are not members of the genus Rymovirus. Phytopathology 88:782-787. The complete nucleotide sequence of wheat streak mosaic virus (WSMV) has been determined based on complementary DNA clones derived from the 9,384-nucleotide (nt) RNA of the virus. The genome of WSMV has a 130-nt 5′ leader and 149-nt 3′-untranslated region and is polyadenylated at the 3′ end. WSMV RNA encodes a single polyprotein of 3,035 amino acid residues and has a deduced genome organization typical for a member of the family Potyviridae (5′-P1/HC-Pro/P3/6K1/CI/6K2/VPg-NIa/NIb/CP-3′). Because WSMV shares with ryegrass mosaic virus (RGMV) the biological property of transmission by eriophyid mites, WSMV has been assigned to the genus Rymovirus, of which RGMV is the type species. Phylogenetic analyses were conducted with complete polyprotein or NIb protein sequences of 11 members of the family Potyviridae, including viruses of monocots or dicots and viruses transmitted by aphids, whiteflies, and mites. WSMV and the monocot-infecting, mite-transmitted brome streak mosaic virus (BrSMV) are sister taxa and share a most recent common ancestor with the whitefly-transmitted sweet potato mild mottle virus, the type species of the proposed genus “Ipomovirus.” In contrast, RGMV shares a most recent common ancestor with aphid-transmitted species of the genus Potyvirus. These results indicate that WSMV and BrSMV should be classified within a new genus of the family Potyviridae and should not be considered species of the genus Rymovirus.
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Molecular cloning, sequencing, and phylogenetic relationships of a new potyvirus: sugarcane streak mosaic virus, and a reevaluation of the classification of the Potyviridae. Mol Phylogenet Evol 10: 323–332
1998Co-Authors: Jeffrey S Hall, Roy French, Thomas J Parsons, Leslie C Lane, Byron Adams, Stanley G JensenAbstract:The nucleic acid of a serologically distinct potyvirus, originally isolated out of sugar cane from Pakistan, was reverse transcribed and the 38 terminal 2000 bp was PCR amplified, cloned, and sequenced. Phyloge-netic comparisons of viruses representing each genus of the Potyviridae show that the Pakistani isolate is most closely related to the Rymoviruses wheat streak mosaic virus (WSMV) and brome streak mosaic virus. We therefore propose that this new virus species be named sugar cane streak mosaic virus to reflect its similarity to WSMV. The phylogenetic data also show that the genus Rymovirus contains at least two unique evolutionary lineages. Thus the current taxonomy, based on transmission vector, is paraphyletic. We pre-sent an analysis of the taxonomic relationships among members of the family and propose a classification that both resolves the paraphyly and more accurately represents the evolutionary history of the Potyviridae. r 1998 Academic Pres
Hanshenning Steinbiss - One of the best experts on this subject based on the ideXlab platform.
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Downloaded from www.microbiologyresearch.org by
2016Co-Authors: Short Communication, Christoph Reichel, C. Maas, Jeff Schell, S. Schulze, Hanshenning SteinbissAbstract:Cooperative binding to nucleic acids by barley yellow mosaic bymovirus coat protein and characterization of a nucleic acid-binding domai
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a large duplication in the 3 untranslated region of a subpopulation of rna2 of the uk m isolate of barley mild mosaic bymovirus
Virus Research, 1997Co-Authors: Ellen Peerenboom, Volker Jacobi, Ewen J Cartwright, Hanshenning Steinbiss, M J Adams, John F. AntoniwAbstract:The UK-M isolate of the bipartite barley mild mosaic bymovirus (BaMMV UK-M) cannot be fungally transmitted, and has previously been shown to have a 1092 nt deletion in the coding region of RNA2. We now report, using sequence and reverse transcriptase-polymerase chain reaction (RT-PCR) data, that a subpopulation of BaMMV UK-M RNA2 contains a direct imperfect sequence repeat of 552 nt in the 3' untranslated region. The secondary structure of the 3' end of RNA2, and its possible effects on replication of the virus, are also discussed.
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COOPERATIVE BINDING TO NUCLEIC ACIDS BY BARLEY YELLOW MOSAIC BYMOVIRUS COAT PROTEIN AND CHARACTERIZATION OF A NUCLEIC ACID-BINDING DOMAIN
Journal of General Virology, 1996Co-Authors: Christoph Reichel, C. Maas, S. C. Schulze, Jeff Schell, Hanshenning SteinbissAbstract:The capacity of several coat protein (CP) mutants of a German isolate of barley yellow mosaic bymovirus (BaYMV) to bind to nucleic acids was studied in vitro. Recombinant CP, produced by overexpression in Escherichia coli, was purified from inclusion bodies and subsequently renatured. Binding to single-stranded (ss) RNA and ssDNA oligonucleotides was found to be cooperative and sequence non-specific. By deletion mutagenesis, several truncated CP derivatives were created and their nucleic acid-binding capacity was investigated in order to define a protein domain responsible for RNA- and DNA-binding. The nucleic acid-binding domain consists of a core which was located to an internal 23 amino acid peptide (aa 125–147) and an adjacent domain (aa 148–184) which stimulates binding.
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the complete nucleotide sequence of rna 2 of a fungally transmitted uk isolate of barley mild mosaic bymovirus and identification of amino acid combinations possibly involved in fungus transmission
Virus Research, 1996Co-Authors: Ellen Peerenboom, Volker Jacobi, John F. Antoniw, Ursula Schlichter, Ewen J Cartwright, Hanshenning Steinbiss, M J AdamsAbstract:Abstract The complete nucleotide sequence of RNA-2 of a fungally-transmitted UK isolate of barley mild mosaic bymovirus (BaMMV isolate UK-F) was determined and compared with other published sequences, particularly UK-M, an isolate derived from the same source but which has been mechanically passaged for several years, has a deletion of about 1 kb and cannot be fungally transmitted. From an alignment of the BaMMV RNA-2 encoded protein with that for barley yellow mosaic bymovirus (BaYMV), several regions of consistent homology were identified and extensive searches made for similarities with the proteins of other fungally-transmitted viruses, especially amongst the furovirus capsid readthrough proteins which seem especially prone to deletion and which have already been implicated in fungus transmission. The amino acid combinations ER (glutamic acid-arginine) or QR (glutamine-arginine) were found consistently in all of the viruses. They occurred in positions predicted to be on the outside of the protein, and therefore available for interaction with the fungus vector, and were also within the regions prone to spontaneous deletion. In view of the lack of other structural or sequence homologies, it is suggested that these motifs are strong candidates for involvement in fungus transmission.
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cloning and sequence analysis of rna 2 of a mechanically transmitted uk isolate of barley mild mosaic bymovirus bammv
Virus Research, 1995Co-Authors: Volker Jacobi, Ellen Peerenboom, John F. Antoniw, Hanshenning Steinbiss, Peer M Schenk, M J AdamsAbstract:A mutant of the 'Streatley' isolate of barley mild mosaic bymovirus was selected from the original field isolate by repeated mechanical incoulation. Unlike the wild-type barley mild mosaic virus, which is transmitted by the soilborne fungus Polymyxa graminis, the mutant could not be transmitted by this vector. RNA-2 of the mutant virus was shorter than that of the wild-type virus suggesting that a deletion of part of the genome segment had occurred. The nucleotide sequence of the mutant RNA-2 was determined and revealed a high degree of homology with the RNA-2 of a German BaMMV field isolate. The deletion comprises 1092 nucleotides and is located in the 3′-terminal part of the coding region. The 34-kDa truncated form of the C-terminal protein is less than half the size of the corresponding protein of full-length BaMMV RNA-2. Taken together, the sequence data and results of biological experiments suggest an essential role of the C-terminal protein for fungus transmission of BaMMV.