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Bertil Romner - One of the best experts on this subject based on the ideXlab platform.
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traumatic brain damage serum s 100 Protein measurements related to neuroradiological findings
Journal of Neurotrauma, 2000Co-Authors: Bertil Romner, Tor Ingebrigtsen, Poul Kongstad, S E BorgesenAbstract:This study was designed to investigate the correlation between S-100 Protein serum measurements and neuroradiological findings in patients with head injury. We studied 278 patients with minor, moderate, and severe head injuries and 110 controls with no history of neurological disease. The study recruited patients from three Scandinavian neurotrauma centers. Serum levels of S-100 Protein were measured at admittance, and computed tomographic scans of the brain were obtained within 24 h postinjury in all patients. In a subgroup of 45 patients with minor head injuries, magnetic resonance imaging was also performed. Increased serum level of S-100 Protein was detected in 108 (39%) patients, and CT scan demonstrated intracranial pathology in 25 (9%) (brain contusion n = 13, subdural hematoma n = 6, epidural hematoma n = 2, traumatic subarachnoid hemorrhage n = 2, and brain edema n = 2). The proportion of patients with detectable serum level was significantly (p < 0.01) higher among those with intracranial pathol...
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the clinical value of serum s 100 Protein measurements in minor head injury a scandinavian multicentre study
Brain Injury, 2000Co-Authors: Tor Ingebrigtsen, Bertil Romner, S Marupjensen, M Dons, Christofer Lundqvist, Johan Bellner, Christer Alling, S E BorgesenAbstract:Purpose: This study of patients with minor head injury was designed to investigate the relation of S-100 Protein measurements to computed tomograpy (CT) findings and patients outcomes. Increased serum levels of this Protein were hypothetized to predict intracranial pathology and increased frequency of post-concussion symptoms. Methods: One hundred and eighty-two patients were studied with Glasgow Coma Scale scores of 13-15. The study recruited patients from three Scandinavian neurotrauma centres. Serum levels of S-100 Protein were measured at admittance and CT scans of the brain were obtained within 24 hours postinjury in all patients. Outcome was evaluated with the Rivermead Postconcussion Symptoms Questionnaire (RPQ) 3 months after the injury. Results: Increased serum level of S-100 Protein was detected in 69 (38%) patients, and CT scan demonstrated intracranial pathology in 10 (5%) (brain contusion in seven, epidural haematoma in two, traumatic subarachnoid haemorrhage in one). The proportion of patien...
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the clinical value of serum s 100 Protein measurements in minor head injury a scandinavian multicentre study
Brain Injury, 2000Co-Authors: Tor Ingebrigtsen, Bertil Romner, S Marupjensen, M Dons, Christofer Lundqvist, Johan Bellner, Christer Alling, S E BorgesenAbstract:PURPOSE: This study of patients with minor head injury was designed to investigate the relation of S-100 Protein measurements to computed tomograpy (CT) findings and patients outcomes. Increased serum levels of this Protein were hypothetized to predict intracranial pathology and increased frequency of post-concussion symptoms. METHODS: One hundred and eighty-two patients were studied with Glasgow Coma Scale scores of 13-15. The study recruited patients from three Scandinavian neurotrauma centres. Serum levels of S-100 Protein were measured at admittance and CT scans of the brain were obtained within 24 hours postinjury in all patients. Outcome was evaluated with the Rivermead Postconcussion Symptoms Questionnaire (RPQ) 3 months after the injury. RESULTS: Increased serum level of S-100 Protein was detected in 69 (38%) patients, and CT scan demonstrated intracranial pathology in 10 (5%) (brain contusion in seven, epidural haematoma in two, traumatic subarachnoid haemorrhage in one). The proportion of patients with detectable serum level was significantly (p < 0.01) higher among those with intracranial pathology (90%) compared to those without (35%). The negative predictive value of an undetectable S-100 level was 0.99. Sixty-two per cent reported one or more post-concussion symptoms at follow-up. A trend was observed towards an increased frequency of post-concussion symptoms among patients with detectable serum levels. CONCLUSIONS: Undetectable serum level of S-100 Protein predicts normal intracranial findings on CT scan. Determination of S-100 Protein in serum may be used to select patients for CT scanning. Increased S-100 serum levels may be more related to post-concussion symptoms caused by mild traumatic brain injury than to symptoms of psychological origin.
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traumatic brain damage in minor head injury relation of serum s 100 Protein measurements to magnetic resonance imaging and neurobehavioral outcome
Neurosurgery, 1999Co-Authors: Tor Ingebrigtsen, Knut Waterloo, E A Jacobsen, Bodil Langbakk, Bertil RomnerAbstract:OBJECTIVE: The present study was conducted to validate S-100 Protein as a marker of brain damage after minor head injury. METHODS: We studied 50 patients with minor head injuries and Glasgow Coma Scale scores of 13 to 15 in whom computed tomographic scans of the brain revealed no abnormalities. Serum levels of S-100 Protein were measured at admittance and hourly thereafter until 12 hours after injury. Magnetic resonance imaging and baseline neuropsychological examinations were performed within 48 hours, and neuropsychological follow-up was conducted at 3 months postinjury. RESULTS: Fourteen patients (28%) had detectable serum levels of S-100 Protein (mean peak value, 0.4 μg/L [standard deviation, ± 0.3]). The S-100 Protein levels were highest immediately after the trauma, and they declined each hour thereafter. At 6 hours postinjury, the serum level was below the detection limit (0.2 μg/L) in five (36%) of the patients with initially detectable levels. Magnetic resonance imaging revealed brain contusions in five patients, four of whom demonstrated detectable levels of S-100 Protein in serum. The proportion of patients with detectable serum levels was significantly higher when magnetic resonance imaging revealed a brain contusion. In patients with detectable serum levels, we observed a trend toward impaired neuropsychological functioning on measures of attention, memory, and information processing speed. CONCLUSION: Determination of S-100 Protein levels in serum provides a valid measure of the presence and severity of traumatic brain damage if performed within the first hours after minor head injury.
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Age- and Sex-Related Changes of S-100 Protein Concentrations in Cerebrospinal Fluid and Serum in Patients with No Previous History of Neurological Disorder
Clinical chemistry, 1997Co-Authors: Øystein P. Nygaard, Bodil Langbakk, Bertil RomnerAbstract:S-100 is a calcium-binding Protein synthesized in astroglial cells in all parts of the central nervous system (CNS). It is present in the body in different subchains, of which the beta form (96%) predominates in the brain (1). S-100 Protein is normally not detectable in serum (1), but previous studies have demonstrated that increased S-100 concentrations in cerebrospinal fluid (CSF) are an index of the active phase of cell injury in patients with acute multiple sclerosis exacerbations, intracranial tumors, acute encephalomyelitis, and spinal cord compression (2). High CSF concentrations of the S-100 Protein have also been demonstrated in patients with glioblastoma, cervical compression, polyneuropathy, hydrocephalus, subarachnoid hemorrhage, encephalitis, meningitis, and cerebral infarction (3)(4)(5)(6)(7)(8). A previous study demonstrated age-related reference values for S-100 Protein in CSF in children and adults with distinct neurological disorders (9). We sampled serum and CSF from 75 men and 35 women undergoing various surgical procedures in spinal anesthesia.The patients had no actual or previous history of neurological disease. The study was performed to establish reference intervals of S-100 Protein in CSF and serum. From August 1995 to June 1996, serum and CSF samples were obtained from 110 patients undergoing surgery in spinal anesthesia. Before inclusion in the study, the patients answered a questionnaire concerning known neurological symptoms or diseases, and their hospital records were investigated. The inclusion criteria were as follows: no history of previous neurological symptoms or disease, no previous investigation in a neurological department, no present symptoms indicating any neurological disease, no evidence of malignant disease, …
Richard A. Komorowski - One of the best experts on this subject based on the ideXlab platform.
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evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma higher diagnostic accuracy with melan a and mart 1 compared with s 100 Protein and hmb 45
The American Journal of Surgical Pathology, 2001Co-Authors: Vinod B. Shidham, Scott Acker, Bal Kampalath, Chung Che Chang, Varghese George, Dan Yi Qi, Richard A. KomorowskiAbstract:Accurate diagnosis of micrometastases in sentinel lymph nodes of cutaneous melanoma is critical for proper clinical management. S-100 Protein and HMB-45 are the traditional immunomarkers widely used for this purpose. However, the interpretation of micrometastases by these markers is difficult with s
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Evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma: higher diagnostic accuracy with Melan-A and MART-1 compared with S-100 Protein and HMB-45.
The American journal of surgical pathology, 2001Co-Authors: Vinod B. Shidham, Scott Acker, Bal Kampalath, Chung Che Chang, Varghese George, Richard A. KomorowskiAbstract:Accurate diagnosis of micrometastases in sentinel lymph nodes of cutaneous melanoma is critical for proper clinical management. S-100 Protein and HMB-45 are the traditional immunomarkers widely used for this purpose. However, the interpretation of micrometastases by these markers is difficult with significant reduction in the diagnostic accuracy. S-100 Protein demonstrates immunoreactivity for other nonmelanoma cells and obscures nuclear details, which are crucial for the interpretation of single cell metastases. We compared the new melanoma markers, Melan-A (clone A103) and MART-1 (clone M2-7C10), with S-100 Protein and HMB-45, by examining 77 formalin-fixed paraffin-embedded sections of sentinel lymph nodes from 13 cases of primary cutaneous melanoma. CD68 (PG-M1) and hematoxylin-eosin-stained sections were also studied. Four pathologists interpreted the staining pattern after concealing the identity of each immunomarker. Az values (area under receiver operating characteristic curve) with receiver operating characteristic curve were higher with Melan-A (0.9742) and MART-1 (0.9779) compared with S-100 Protein (0.8034) and HMB-45 (0.8651), demonstrating a higher diagnostic accuracy with Melan-A and MART-1 with superior detection of melanoma micrometastases. Melan-A and MART-1 showed sharp cytoplasmic immunoreactivity, almost exclusively restricted to the melanoma cells. Therefore, Melan-A and MART-1 are recommended for the evaluation of micrometastases in sentinel lymph nodes of cutaneous melanoma as a routine alternative to S-100 Protein and HMB-45.
K J B Lamers - One of the best experts on this subject based on the ideXlab platform.
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intraoperative values of s 100 Protein myelin basic Protein lactate and albumin in the csf and serum of neurosurgical patients
Journal of Neurology Neurosurgery and Psychiatry, 2001Co-Authors: J De Vries, W A M H Thijssen, S E Snels, T Menovsky, N G M Peer, K J B LamersAbstract:OBJECTIVES To assess the concentrations of S-100 Protein, myelin basic Protein (MBP), and lactate, and the (CSF)/serum albumin ratio (Qalb) during intracranial neurosurgical procedures. METHODS Samples of CSF from 91 patients with various CNS diseases were obtained by aspiration of cisternal CSF at the beginning of surgery (before starting surgical manipulation of the brain) and concentrations of S-100 Protein, MBP, and lactate, and Qalb were determined. At the same time blood was sampled for determination of serum S-100 Protein concentration. Patients were divided into three groups according to the aetiology of their CNS disease (intracranial haemorrhage, n=11; benign intracranial mass lesion, n=52; malignant neoplastic disease, n=28). Radiological and intraoperative characteristics were documented. RESULTS In each of these three groups median values of all four CSF variables measured were raised. The occurrence of brain oedema and a midline shift correlated significantly with raised concentrations of MBP and Qalb. Breaching of the arachnoid layer, documented at surgery for benign lesions, correlated with higher concentrations of MBP, lactate, CSF S-100 Protein, and Qalb. CONCLUSIONS Intraoperative values of S-100 Protein, MBP, lactate, and Qalb are increased in patients with intracranial haemorrhage, benign intracranial mass lesion, and malignant neoplastic disease. Breaching of the arachnoid layer and oedema is associated with higher concentrations of some of the aforementioned Proteins. These biochemical data can serve as a basis for further research into CSF specific Proteins.
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age related changes of neuron specific enolase s 100 Protein and myelin basic Protein concentrations in cerebrospinal fluid
Clinical Chemistry, 1992Co-Authors: B G M Van Engelen, K J B Lamers, F J M Gabreels, Ron A Wevers, W J A Van Geel, George F BormAbstract:Studies on cerebrospinal fluid (CSF) concentrations of neuron-specific enolase (NSE), S-100 Protein, and myelin basic Protein (MBP) in patients with neurological lesions indicate a quantitative relation between the degree of cell damage in the central nervous system (CNS) and the concentration of these CNS-specific Proteins in CSF. Thus NSE, S-100, and MBP could be of use as markers for destructive processes in the CNS. We collected 937 specimens of CSF from children and adults (from newborns to age 91 years) who were undergoing a diagnostic lumbar puncture for several clinical indications. Of these, 79 samples from subjects ranging in age from 0.7 to 66 years could be used retrospectively to construct a reference interval according to our criteria. In these 79 samples no sex dependency existed. The relative increase of NSE, S-100, and MBP with age was similar (1% per year), suggesting a common underlying mechanism. These results emphasize the necessity of using age-matched reference values when the CNS-specific Proteins are to be evaluated in neurological diseases. We also present three case histories to discuss the possible clinical relevance of the measurement of NSE, S-100, and MBP in children and adults.
Tor Ingebrigtsen - One of the best experts on this subject based on the ideXlab platform.
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traumatic brain damage serum s 100 Protein measurements related to neuroradiological findings
Journal of Neurotrauma, 2000Co-Authors: Bertil Romner, Tor Ingebrigtsen, Poul Kongstad, S E BorgesenAbstract:This study was designed to investigate the correlation between S-100 Protein serum measurements and neuroradiological findings in patients with head injury. We studied 278 patients with minor, moderate, and severe head injuries and 110 controls with no history of neurological disease. The study recruited patients from three Scandinavian neurotrauma centers. Serum levels of S-100 Protein were measured at admittance, and computed tomographic scans of the brain were obtained within 24 h postinjury in all patients. In a subgroup of 45 patients with minor head injuries, magnetic resonance imaging was also performed. Increased serum level of S-100 Protein was detected in 108 (39%) patients, and CT scan demonstrated intracranial pathology in 25 (9%) (brain contusion n = 13, subdural hematoma n = 6, epidural hematoma n = 2, traumatic subarachnoid hemorrhage n = 2, and brain edema n = 2). The proportion of patients with detectable serum level was significantly (p < 0.01) higher among those with intracranial pathol...
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the clinical value of serum s 100 Protein measurements in minor head injury a scandinavian multicentre study
Brain Injury, 2000Co-Authors: Tor Ingebrigtsen, Bertil Romner, S Marupjensen, M Dons, Christofer Lundqvist, Johan Bellner, Christer Alling, S E BorgesenAbstract:Purpose: This study of patients with minor head injury was designed to investigate the relation of S-100 Protein measurements to computed tomograpy (CT) findings and patients outcomes. Increased serum levels of this Protein were hypothetized to predict intracranial pathology and increased frequency of post-concussion symptoms. Methods: One hundred and eighty-two patients were studied with Glasgow Coma Scale scores of 13-15. The study recruited patients from three Scandinavian neurotrauma centres. Serum levels of S-100 Protein were measured at admittance and CT scans of the brain were obtained within 24 hours postinjury in all patients. Outcome was evaluated with the Rivermead Postconcussion Symptoms Questionnaire (RPQ) 3 months after the injury. Results: Increased serum level of S-100 Protein was detected in 69 (38%) patients, and CT scan demonstrated intracranial pathology in 10 (5%) (brain contusion in seven, epidural haematoma in two, traumatic subarachnoid haemorrhage in one). The proportion of patien...
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the clinical value of serum s 100 Protein measurements in minor head injury a scandinavian multicentre study
Brain Injury, 2000Co-Authors: Tor Ingebrigtsen, Bertil Romner, S Marupjensen, M Dons, Christofer Lundqvist, Johan Bellner, Christer Alling, S E BorgesenAbstract:PURPOSE: This study of patients with minor head injury was designed to investigate the relation of S-100 Protein measurements to computed tomograpy (CT) findings and patients outcomes. Increased serum levels of this Protein were hypothetized to predict intracranial pathology and increased frequency of post-concussion symptoms. METHODS: One hundred and eighty-two patients were studied with Glasgow Coma Scale scores of 13-15. The study recruited patients from three Scandinavian neurotrauma centres. Serum levels of S-100 Protein were measured at admittance and CT scans of the brain were obtained within 24 hours postinjury in all patients. Outcome was evaluated with the Rivermead Postconcussion Symptoms Questionnaire (RPQ) 3 months after the injury. RESULTS: Increased serum level of S-100 Protein was detected in 69 (38%) patients, and CT scan demonstrated intracranial pathology in 10 (5%) (brain contusion in seven, epidural haematoma in two, traumatic subarachnoid haemorrhage in one). The proportion of patients with detectable serum level was significantly (p < 0.01) higher among those with intracranial pathology (90%) compared to those without (35%). The negative predictive value of an undetectable S-100 level was 0.99. Sixty-two per cent reported one or more post-concussion symptoms at follow-up. A trend was observed towards an increased frequency of post-concussion symptoms among patients with detectable serum levels. CONCLUSIONS: Undetectable serum level of S-100 Protein predicts normal intracranial findings on CT scan. Determination of S-100 Protein in serum may be used to select patients for CT scanning. Increased S-100 serum levels may be more related to post-concussion symptoms caused by mild traumatic brain injury than to symptoms of psychological origin.
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traumatic brain damage in minor head injury relation of serum s 100 Protein measurements to magnetic resonance imaging and neurobehavioral outcome
Neurosurgery, 1999Co-Authors: Tor Ingebrigtsen, Knut Waterloo, E A Jacobsen, Bodil Langbakk, Bertil RomnerAbstract:OBJECTIVE: The present study was conducted to validate S-100 Protein as a marker of brain damage after minor head injury. METHODS: We studied 50 patients with minor head injuries and Glasgow Coma Scale scores of 13 to 15 in whom computed tomographic scans of the brain revealed no abnormalities. Serum levels of S-100 Protein were measured at admittance and hourly thereafter until 12 hours after injury. Magnetic resonance imaging and baseline neuropsychological examinations were performed within 48 hours, and neuropsychological follow-up was conducted at 3 months postinjury. RESULTS: Fourteen patients (28%) had detectable serum levels of S-100 Protein (mean peak value, 0.4 μg/L [standard deviation, ± 0.3]). The S-100 Protein levels were highest immediately after the trauma, and they declined each hour thereafter. At 6 hours postinjury, the serum level was below the detection limit (0.2 μg/L) in five (36%) of the patients with initially detectable levels. Magnetic resonance imaging revealed brain contusions in five patients, four of whom demonstrated detectable levels of S-100 Protein in serum. The proportion of patients with detectable serum levels was significantly higher when magnetic resonance imaging revealed a brain contusion. In patients with detectable serum levels, we observed a trend toward impaired neuropsychological functioning on measures of attention, memory, and information processing speed. CONCLUSION: Determination of S-100 Protein levels in serum provides a valid measure of the presence and severity of traumatic brain damage if performed within the first hours after minor head injury.
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serial s 100 Protein serum measurements related to early magnetic resonance imaging after minor head injury
Journal of Neurosurgery, 1996Co-Authors: Tor Ingebrigtsen, Bertil RomnerAbstract:✓ The authors studied 24 patients with a Glasgow Coma Scale score of 14 or 15 and normal computerized tomography scans after minor head injury. The study protocol included obtaining serial measurements of S-100 Protein in serum during the first 12 hours after injury and early magnetic resonance (MR) imaging. Four patients (17%) had detectable levels of S-100 Protein in serum. The S-100 Protein levels were highest immediately after trauma, declining hour by hour. In two patients, MR imaging revealed intracranial contusion. Levels of S-100 Protein were not detectable in serum in one patient with MR-verified cerebral contusion, but the first measurements were made late, 6 hours after trauma. The highest serum level of S-100 Protein (0.9 µg/L) was seen in a 73-year-old man 2 hours after injury. Magnetic resonance imaging revealed a contusion of the left cerebellar hemisphere, and the patient suffered permanent sequelae of impaired posture and dizziness.
Achim A Jungbluth - One of the best experts on this subject based on the ideXlab platform.
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analysis of microphthalmia transcription factor expression in normal tissues and tumors and comparison of its expression with s 100 Protein gp100 and tyrosinase in desmoplastic malignant melanoma
The American Journal of Surgical Pathology, 2001Co-Authors: Klaus J Busam, Kristin Iversen, Keren Coplan, Achim A JungbluthAbstract:Microphthalmia transcription factor (Mitf) is a nuclear Protein involved in the development of melanocytes and the regulation of melanin synthesis. Recent studies have suggested that Mitf may be a more sensitive and specific melanocyte marker than S-100 Protein and gp100. However, there is insufficient knowledge on the specificity of Mitf, and a systematic examination of its use for the recognition of desmoplastic melanoma has not yet been performed. In this study, we compared the expression of Mitf with S-100 Protein, gp100, and tyrosinase in 20 desmoplastic melanomas by using the antibodies D5 (anti-Mitf), anti-S100P, HMB-45 (anti-gp100), and T311 (anti-tyrosinase). All 20 melanomas were positive for S-100 Protein, 7 were positive for Mitf, 6 for gp100, and 11 for tyrosinase. To examine the specificity of Mitf, a panel of normal tissue and 386 samples of miscellaneous tumors, including dermal and subcutaneous spindle cell lesions relevant for the differential diagnosis of desmoplastic melanoma, were examined by immunohistochemistry. Furthermore, normal tissue samples were tested for Mitf mRNA by reverse transcriptase polymerase chain reaction (rt-PCR). Immunoreactivity for Mitf was seen not only in melanocytes of normal skin, but also in macrophages, lymphocytes, fibroblasts, Schwann cells, and smooth muscle cells at various sites, and tumors derived thereof. Our results indicate that the antibody D5 lacks sufficient sensitivity and specificity for widespread diagnostic use. Especially in re-excisions, when immunohistochemistry is often needed to distinguish an inflamed scar tissue from tumor, the presence of immunopositive inflammatory cells and fibroblasts limits the diagnostic use of D5.
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analysis of microphthalmia transcription factor expression in normal tissues and tumors and comparison of its expression with s 100 Protein gp100 and tyrosinase in desmoplastic malignant melanoma
The American Journal of Surgical Pathology, 2001Co-Authors: Klaus J Busam, Kristin Iversen, Keren Coplan, Achim A JungbluthAbstract:Microphthalmia transcription factor (Mitf) is a nuclear Protein involved in the development of melanocytes and the regulation of melanin synthesis. Recent studies have suggested that Mitf may be a more sensitive and specific melanocyte marker than S-100 Protein and gp100. However, there is insuffici
