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R K Kohli - One of the best experts on this subject based on the ideXlab platform.
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assessment of in vitro antioxidant activity of essential oil of eucalyptus citriodora lemon scented eucalypt myrtaceae and its major constituents
Lwt - Food Science and Technology, 2012Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Kirti Negi, Savita Kumari, Varinder Saini, R K KohliAbstract:Abstract We investigated the chemical composition and antioxidant activity of Eucalyptus citriodora (lemon-scented eucalyptus) leaf oil in terms of total antioxidant activity, ferric reducing antioxidant power (FRAP) assay, ferrous ion chelating activity, and scavenging of hydrogen peroxide (H 2 O 2 ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radicals and inhibition of lipid peroxidation. GC–MS analysis of essential oil revealed the presence of 43 components constituting 99.2% of oil. The oil was monoterpenoid (94.35% of oil) with citronellal (60.66%), β -citronellol (12.58%) and isopulegol (8.19%) as the major monoterpenoids. Oil and its major monoterpenes exhibited moderate to strong antioxidant activity in terms of TAA, FRAP and Fe +2 chelating, DPPH and H 2 O 2 scavenging, and lipid peroxidation inhibition. The study concludes that E. citriodora leaves contain monoterpenoid rich oil exhibiting antioxidant activity.
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chemical composition and antioxidant activity of essential oil from residues of artemisia scoparia
Food Chemistry, 2009Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Sunil Mittal, R K KohliAbstract:Abstract The chemical composition of hydrodistilled oil (yield ∼0.17%, w/v), from the residues of Artemisia scoparia Waldst. & Kit. (sagebrush or wormweed), was analysed for the first time by GC/GC–MS. Of the 49 compounds present in the oil, 48, accounting for 99.28% of the oil, were identified. The volatile oil contained 24 monoterpenoids (56.7%), 19 sesquiterpenoids (28.7%), 2 ketones (0.25%), 1 ester (1.87%), 1 chromene (precocene II, 0.65%) and a hydrocarbon compound. Citronellal (15.2%) followed by β-citronellol (11%) were the major monoterpene constituents of the oil. The residue essential oils (25–200 μg/ml) exhibited a strong antioxidant and radical scavenging activity against hydroxyl ion (OH ) and hydrogen peroxide (H 2 O 2 ). This study concludes that residues of A. scoparia could serve as an important bioresource for extraction of monoterpenoid-rich oil exhibiting antioxidant activity, and thus hold a good potential for use in the food and pharmaceutical industry.
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chemical composition and phytotoxicity of volatile essential oil from intact and fallen leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A total of 23 volatile constituents was identified and characterized by GC and GC-MS in the volatile essential oil extracted from intact (juvenile and adult) and fallen (senescent and leaf litter) leaves of lemon-scented eucalyptus (Eucalyptus citriodora Hook.). The leaves differed in their pigment, water and protein content, and C/N ratio. The oils were, in general, monoterpenoid in nature with 18 monoterpenes and 5 sesquiterpenes. However, a great variability in the amount of essential oils and their individual constituents was observed in different leaf tissues. The amount was maximum in the senescent leaves collected from the floor of the tree closely followed by that from juvenile leaves. In all, 19 constituents were identified in oil from juvenile and senescent leaves compared to 23 in adult leaves and 20 in leaf litter, respectively. Citronellal, a characteristic monoterpene of the oil reported hitherto was found to be more (77-78%) in the juvenile and senescent leaves compared to 48 and 54%, respectively, in the adult leaves and leaf litter. In the adult leaves, however, the content of citronellol--another important monoterpene-- was very high (21.9%) compared to other leaf types (7.8-12.2%). Essential oil and its two major monoterpenes viz. citronellal and citronellol were tested for their phytotoxicity against two weeds (Amaranthus viridis and Echinochloa crus-galli) and two crops (Triticum aestivum and Oryza sativa) under laboratory conditions. A difference in the phytotoxicity, measured in terms of seedling length and dry weight, of oil from different leaves and major monoterpenes was observed. Oil from adult leaves was found to be most phytotoxic although it occurs in smaller amount (on unit weight basis). The different toxicity of different oil types was due to the relative amount of individual monoterpenes present in the oil, their solubility and interactive action. The study concludes that oil from senescent and juvenile leaves being rich in citronellal could be used as commercial source of citronellal whereas that from adult leaves for weed management programmes as it was the most phytotoxic.
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chemical composition and inhibitory activity of essential oil from decaying leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A study was undertaken to explore the content and composition of volatile oil from decaying leaves of lemon-scented eucalypt (Eucalyptus citriodora Hook.) not analyzed earlier. GC and GC-MS analysis of the oil (yield 0.6%) revealed the monoterpenoid nature with citronellal (52.2%), citronellol (12.3%) and isoisopulegol (11.9%) as the major constituents. Overall, 17 components were identified that accounted for over 94% of the decaying leaf oil. Surprisingly, the decaying leaf oil contained nearly 1.8% of trans-rose oxide, which is generally absent in eucalypt essential oil. Decaying leaf oil and its major 2 components (citronellal and citronellol) inhibited the germination and root elongation of two weeds--Cassia occidentalis (broad-leaved) and Echinochloa crus-galli (grassy weed). Based on the dose-response studies, I50 values were determined for decaying leaf oil and the effect was more on germination only of broad-leaved weed (C. occidentalis), whereas that of citronellal and citronellol were on germination as well as root length of E. crus-galli (grassy weed). Based on I50 values it was observed that citronellal was more phytotoxic and germination inhibiting in nature, whereas citronellol was a more potent root inhibitor, thereby indicating a possible different mode of action. The study concludes that decaying leaf oil hold a good commercial value for exploitation as weed management agent.
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comparative phytotoxicity of four monoterpenes against cassia occidentalis
Annals of Applied Biology, 2002Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, H Ramezani, R K KohliAbstract:The effect of four monoterpenes - citronellol, citronellal, cineole and linalool - on the germination, growth and physiology of Cassia occidentalis was investigated. All four monoterpenes reduced germination of C. occidentalis seeds but to varying extents. Citronellal and linalool completely inhibited germination beyond the concentrations of 55 and 110 μM, respectively, whereas in response to treatment of citronellol no germination was observed beyond 330 μM. Further, the growth of seedlings, measured in terms of seedling length and biomass, was also adversely affected. A reduction in chlorophyll content of the cotyledonary leaves of C. occidentalis was also noticed, indicating an adverse effect on photosynthesis. Likewise, respiratory ability of growing seeds was also impaired in response to all four monoterpenes, clearly indicating that monoterpenes affect energy metabolism. On the basis of overall phytotoxicity, potency of monoterpenes was in the order of citronellal > linalool > citronellol > cineole. The results from this study suggest that both citronellal and linalool possess strong phytotoxic potential and can thus serve as lead molecules for the synthesis of bioherbicides.
Krystyna Skalicka-woźniak - One of the best experts on this subject based on the ideXlab platform.
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Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt.
BMC complementary and alternative medicine, 2018Co-Authors: Mohamed Z. M. Salem, Hosam O. Elansary, Hayssam M. Ali, Ahmed A. El-settawy, Mohamed Soliman Elshikh, Eslam M. Abdel-salam, Krystyna Skalicka-woźniakAbstract:Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson, widely grown in many subtropical areas, are used for commercial purposes, such as in perfumery, cosmetics, and room fresheners. Their potential as a source of antimicrobial compounds may be useful in different applications. The chemical composition of essential oils (EOs) from C. macrocarpa branchlets and C. citriodora leaves was analyzed by using gas chromatography–mass spectrometry (GC/MS). Antibacterial and antifungal activities were assessed by the micro-dilution method to determine the minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs), and minimum bactericidal concentrations (MBCs). Further, the antioxidant capacity of the EOs was determined via 2,2′-diphenypicrylhydrazyl (DPPH) and β-carotene-linoleic acid assays. Terpinen-4-ol (23.7%), α-phellandrene (19.2%), α-citronellol (17.3%), and citronellal were the major constituents of EO from C. macrocarpa branchlets, and α-citronellal (56%), α-citronellol (14.7%), citronellol acetate (12.3%), isopulegol, and eucalyptol were the primary constituents of EO from C. citriodora leaves. Antibacterial activity with MIC values of EO from C. citriodora leaves was ranged from 0.06 mg/mL to 0.20 mg/mL, and MBC from 0.12 mg/mL against E. coli to 0.41 mg/mL. EO from C. macrocarpa branchlets showed less activity against bacterial strains. The MIC values against tested fungi of the EO from C. citriodora ranged from 0.11 to 0.52 mg/mL while for EO from C. macrocarpa from 0.29 to 3.21 mg/mL. The MIC and MFC values of EOs against P. funiculosum were lower than those obtained from Ketoconazole (KTZ) (0.20; 0.45; 0.29 and 0.53 mg/mL, respectively, vs 0.21 and 0.41 mg/mL. Antioxidant activity of the EO from C. citriodora was higher than that of the positive control but lower than that of the standard butylhydroxytoluene (BHT) (IC50 = 5.1 ± 0.1 μg/mL). The results indicate that the EO from Egyptian trees such as C. citriodora leaves may possesses strong bactericidal and fungicidal activities and can be used as an agrochemical for controlling plant pathogens and in human disease management which will add crop additive value.
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Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt
BMC Complementary and Alternative Medicine, 2018Co-Authors: Mohamed Z. M. Salem, Hosam O. Elansary, Hayssam M. Ali, Ahmed A. El-settawy, Mohamed Soliman Elshikh, Eslam M. Abdel-salam, Krystyna Skalicka-woźniakAbstract:Background Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson, widely grown in many subtropical areas, are used for commercial purposes, such as in perfumery, cosmetics, and room fresheners. Their potential as a source of antimicrobial compounds may be useful in different applications. Methods The chemical composition of essential oils (EOs) from C. macrocarpa branchlets and C. citriodora leaves was analyzed by using gas chromatography–mass spectrometry (GC/MS). Antibacterial and antifungal activities were assessed by the micro-dilution method to determine the minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs), and minimum bactericidal concentrations (MBCs). Further, the antioxidant capacity of the EOs was determined via 2,2′-diphenypicrylhydrazyl (DPPH) and β -carotene-linoleic acid assays. Results Terpinen-4-ol (23.7%), α-phellandrene (19.2%), α -citronellol (17.3%), and citronellal were the major constituents of EO from C. macrocarpa branchlets, and α -citronellal (56%), α -citronellol (14.7%), citronellol acetate (12.3%), isopulegol, and eucalyptol were the primary constituents of EO from C. citriodora leaves. Antibacterial activity with MIC values of EO from C. citriodora leaves was ranged from 0.06 mg/mL to 0.20 mg/mL, and MBC from 0.12 mg/mL against E. coli to 0.41 mg/mL. EO from C. macrocarpa branchlets showed less activity against bacterial strains. The MIC values against tested fungi of the EO from C. citriodora ranged from 0.11 to 0.52 mg/mL while for EO from C. macrocarpa from 0.29 to 3.21 mg/mL. The MIC and MFC values of EOs against P. funiculosum were lower than those obtained from Ketoconazole (KTZ) (0.20; 0.45; 0.29 and 0.53 mg/mL, respectively, vs 0.21 and 0.41 mg/mL. Antioxidant activity of the EO from C. citriodora was higher than that of the positive control but lower than that of the standard butylhydroxytoluene (BHT) (IC_50 = 5.1 ± 0.1 μg/mL). Conclusion The results indicate that the EO from Egyptian trees such as C. citriodora leaves may possesses strong bactericidal and fungicidal activities and can be used as an agrochemical for controlling plant pathogens and in human disease management which will add crop additive value.
Harminder Pal Singh - One of the best experts on this subject based on the ideXlab platform.
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assessment of in vitro antioxidant activity of essential oil of eucalyptus citriodora lemon scented eucalypt myrtaceae and its major constituents
Lwt - Food Science and Technology, 2012Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Kirti Negi, Savita Kumari, Varinder Saini, R K KohliAbstract:Abstract We investigated the chemical composition and antioxidant activity of Eucalyptus citriodora (lemon-scented eucalyptus) leaf oil in terms of total antioxidant activity, ferric reducing antioxidant power (FRAP) assay, ferrous ion chelating activity, and scavenging of hydrogen peroxide (H 2 O 2 ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radicals and inhibition of lipid peroxidation. GC–MS analysis of essential oil revealed the presence of 43 components constituting 99.2% of oil. The oil was monoterpenoid (94.35% of oil) with citronellal (60.66%), β -citronellol (12.58%) and isopulegol (8.19%) as the major monoterpenoids. Oil and its major monoterpenes exhibited moderate to strong antioxidant activity in terms of TAA, FRAP and Fe +2 chelating, DPPH and H 2 O 2 scavenging, and lipid peroxidation inhibition. The study concludes that E. citriodora leaves contain monoterpenoid rich oil exhibiting antioxidant activity.
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chemical composition and antioxidant activity of essential oil from residues of artemisia scoparia
Food Chemistry, 2009Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Sunil Mittal, R K KohliAbstract:Abstract The chemical composition of hydrodistilled oil (yield ∼0.17%, w/v), from the residues of Artemisia scoparia Waldst. & Kit. (sagebrush or wormweed), was analysed for the first time by GC/GC–MS. Of the 49 compounds present in the oil, 48, accounting for 99.28% of the oil, were identified. The volatile oil contained 24 monoterpenoids (56.7%), 19 sesquiterpenoids (28.7%), 2 ketones (0.25%), 1 ester (1.87%), 1 chromene (precocene II, 0.65%) and a hydrocarbon compound. Citronellal (15.2%) followed by β-citronellol (11%) were the major monoterpene constituents of the oil. The residue essential oils (25–200 μg/ml) exhibited a strong antioxidant and radical scavenging activity against hydroxyl ion (OH ) and hydrogen peroxide (H 2 O 2 ). This study concludes that residues of A. scoparia could serve as an important bioresource for extraction of monoterpenoid-rich oil exhibiting antioxidant activity, and thus hold a good potential for use in the food and pharmaceutical industry.
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chemical composition and phytotoxicity of volatile essential oil from intact and fallen leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A total of 23 volatile constituents was identified and characterized by GC and GC-MS in the volatile essential oil extracted from intact (juvenile and adult) and fallen (senescent and leaf litter) leaves of lemon-scented eucalyptus (Eucalyptus citriodora Hook.). The leaves differed in their pigment, water and protein content, and C/N ratio. The oils were, in general, monoterpenoid in nature with 18 monoterpenes and 5 sesquiterpenes. However, a great variability in the amount of essential oils and their individual constituents was observed in different leaf tissues. The amount was maximum in the senescent leaves collected from the floor of the tree closely followed by that from juvenile leaves. In all, 19 constituents were identified in oil from juvenile and senescent leaves compared to 23 in adult leaves and 20 in leaf litter, respectively. Citronellal, a characteristic monoterpene of the oil reported hitherto was found to be more (77-78%) in the juvenile and senescent leaves compared to 48 and 54%, respectively, in the adult leaves and leaf litter. In the adult leaves, however, the content of citronellol--another important monoterpene-- was very high (21.9%) compared to other leaf types (7.8-12.2%). Essential oil and its two major monoterpenes viz. citronellal and citronellol were tested for their phytotoxicity against two weeds (Amaranthus viridis and Echinochloa crus-galli) and two crops (Triticum aestivum and Oryza sativa) under laboratory conditions. A difference in the phytotoxicity, measured in terms of seedling length and dry weight, of oil from different leaves and major monoterpenes was observed. Oil from adult leaves was found to be most phytotoxic although it occurs in smaller amount (on unit weight basis). The different toxicity of different oil types was due to the relative amount of individual monoterpenes present in the oil, their solubility and interactive action. The study concludes that oil from senescent and juvenile leaves being rich in citronellal could be used as commercial source of citronellal whereas that from adult leaves for weed management programmes as it was the most phytotoxic.
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chemical composition and inhibitory activity of essential oil from decaying leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A study was undertaken to explore the content and composition of volatile oil from decaying leaves of lemon-scented eucalypt (Eucalyptus citriodora Hook.) not analyzed earlier. GC and GC-MS analysis of the oil (yield 0.6%) revealed the monoterpenoid nature with citronellal (52.2%), citronellol (12.3%) and isoisopulegol (11.9%) as the major constituents. Overall, 17 components were identified that accounted for over 94% of the decaying leaf oil. Surprisingly, the decaying leaf oil contained nearly 1.8% of trans-rose oxide, which is generally absent in eucalypt essential oil. Decaying leaf oil and its major 2 components (citronellal and citronellol) inhibited the germination and root elongation of two weeds--Cassia occidentalis (broad-leaved) and Echinochloa crus-galli (grassy weed). Based on the dose-response studies, I50 values were determined for decaying leaf oil and the effect was more on germination only of broad-leaved weed (C. occidentalis), whereas that of citronellal and citronellol were on germination as well as root length of E. crus-galli (grassy weed). Based on I50 values it was observed that citronellal was more phytotoxic and germination inhibiting in nature, whereas citronellol was a more potent root inhibitor, thereby indicating a possible different mode of action. The study concludes that decaying leaf oil hold a good commercial value for exploitation as weed management agent.
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comparative phytotoxicity of four monoterpenes against cassia occidentalis
Annals of Applied Biology, 2002Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, H Ramezani, R K KohliAbstract:The effect of four monoterpenes - citronellol, citronellal, cineole and linalool - on the germination, growth and physiology of Cassia occidentalis was investigated. All four monoterpenes reduced germination of C. occidentalis seeds but to varying extents. Citronellal and linalool completely inhibited germination beyond the concentrations of 55 and 110 μM, respectively, whereas in response to treatment of citronellol no germination was observed beyond 330 μM. Further, the growth of seedlings, measured in terms of seedling length and biomass, was also adversely affected. A reduction in chlorophyll content of the cotyledonary leaves of C. occidentalis was also noticed, indicating an adverse effect on photosynthesis. Likewise, respiratory ability of growing seeds was also impaired in response to all four monoterpenes, clearly indicating that monoterpenes affect energy metabolism. On the basis of overall phytotoxicity, potency of monoterpenes was in the order of citronellal > linalool > citronellol > cineole. The results from this study suggest that both citronellal and linalool possess strong phytotoxic potential and can thus serve as lead molecules for the synthesis of bioherbicides.
Mohamed Z. M. Salem - One of the best experts on this subject based on the ideXlab platform.
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Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt.
BMC complementary and alternative medicine, 2018Co-Authors: Mohamed Z. M. Salem, Hosam O. Elansary, Hayssam M. Ali, Ahmed A. El-settawy, Mohamed Soliman Elshikh, Eslam M. Abdel-salam, Krystyna Skalicka-woźniakAbstract:Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson, widely grown in many subtropical areas, are used for commercial purposes, such as in perfumery, cosmetics, and room fresheners. Their potential as a source of antimicrobial compounds may be useful in different applications. The chemical composition of essential oils (EOs) from C. macrocarpa branchlets and C. citriodora leaves was analyzed by using gas chromatography–mass spectrometry (GC/MS). Antibacterial and antifungal activities were assessed by the micro-dilution method to determine the minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs), and minimum bactericidal concentrations (MBCs). Further, the antioxidant capacity of the EOs was determined via 2,2′-diphenypicrylhydrazyl (DPPH) and β-carotene-linoleic acid assays. Terpinen-4-ol (23.7%), α-phellandrene (19.2%), α-citronellol (17.3%), and citronellal were the major constituents of EO from C. macrocarpa branchlets, and α-citronellal (56%), α-citronellol (14.7%), citronellol acetate (12.3%), isopulegol, and eucalyptol were the primary constituents of EO from C. citriodora leaves. Antibacterial activity with MIC values of EO from C. citriodora leaves was ranged from 0.06 mg/mL to 0.20 mg/mL, and MBC from 0.12 mg/mL against E. coli to 0.41 mg/mL. EO from C. macrocarpa branchlets showed less activity against bacterial strains. The MIC values against tested fungi of the EO from C. citriodora ranged from 0.11 to 0.52 mg/mL while for EO from C. macrocarpa from 0.29 to 3.21 mg/mL. The MIC and MFC values of EOs against P. funiculosum were lower than those obtained from Ketoconazole (KTZ) (0.20; 0.45; 0.29 and 0.53 mg/mL, respectively, vs 0.21 and 0.41 mg/mL. Antioxidant activity of the EO from C. citriodora was higher than that of the positive control but lower than that of the standard butylhydroxytoluene (BHT) (IC50 = 5.1 ± 0.1 μg/mL). The results indicate that the EO from Egyptian trees such as C. citriodora leaves may possesses strong bactericidal and fungicidal activities and can be used as an agrochemical for controlling plant pathogens and in human disease management which will add crop additive value.
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Bioactivity of essential oils extracted from Cupressus macrocarpa branchlets and Corymbia citriodora leaves grown in Egypt
BMC Complementary and Alternative Medicine, 2018Co-Authors: Mohamed Z. M. Salem, Hosam O. Elansary, Hayssam M. Ali, Ahmed A. El-settawy, Mohamed Soliman Elshikh, Eslam M. Abdel-salam, Krystyna Skalicka-woźniakAbstract:Background Cupressus macrocarpa Hartw and Corymbia citriodora (Hook.) K.D. Hill & L.A.S. Johnson, widely grown in many subtropical areas, are used for commercial purposes, such as in perfumery, cosmetics, and room fresheners. Their potential as a source of antimicrobial compounds may be useful in different applications. Methods The chemical composition of essential oils (EOs) from C. macrocarpa branchlets and C. citriodora leaves was analyzed by using gas chromatography–mass spectrometry (GC/MS). Antibacterial and antifungal activities were assessed by the micro-dilution method to determine the minimum inhibitory concentrations (MICs), and minimum fungicidal concentrations (MFCs), and minimum bactericidal concentrations (MBCs). Further, the antioxidant capacity of the EOs was determined via 2,2′-diphenypicrylhydrazyl (DPPH) and β -carotene-linoleic acid assays. Results Terpinen-4-ol (23.7%), α-phellandrene (19.2%), α -citronellol (17.3%), and citronellal were the major constituents of EO from C. macrocarpa branchlets, and α -citronellal (56%), α -citronellol (14.7%), citronellol acetate (12.3%), isopulegol, and eucalyptol were the primary constituents of EO from C. citriodora leaves. Antibacterial activity with MIC values of EO from C. citriodora leaves was ranged from 0.06 mg/mL to 0.20 mg/mL, and MBC from 0.12 mg/mL against E. coli to 0.41 mg/mL. EO from C. macrocarpa branchlets showed less activity against bacterial strains. The MIC values against tested fungi of the EO from C. citriodora ranged from 0.11 to 0.52 mg/mL while for EO from C. macrocarpa from 0.29 to 3.21 mg/mL. The MIC and MFC values of EOs against P. funiculosum were lower than those obtained from Ketoconazole (KTZ) (0.20; 0.45; 0.29 and 0.53 mg/mL, respectively, vs 0.21 and 0.41 mg/mL. Antioxidant activity of the EO from C. citriodora was higher than that of the positive control but lower than that of the standard butylhydroxytoluene (BHT) (IC_50 = 5.1 ± 0.1 μg/mL). Conclusion The results indicate that the EO from Egyptian trees such as C. citriodora leaves may possesses strong bactericidal and fungicidal activities and can be used as an agrochemical for controlling plant pathogens and in human disease management which will add crop additive value.
Daizy R. Batish - One of the best experts on this subject based on the ideXlab platform.
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assessment of in vitro antioxidant activity of essential oil of eucalyptus citriodora lemon scented eucalypt myrtaceae and its major constituents
Lwt - Food Science and Technology, 2012Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Kirti Negi, Savita Kumari, Varinder Saini, R K KohliAbstract:Abstract We investigated the chemical composition and antioxidant activity of Eucalyptus citriodora (lemon-scented eucalyptus) leaf oil in terms of total antioxidant activity, ferric reducing antioxidant power (FRAP) assay, ferrous ion chelating activity, and scavenging of hydrogen peroxide (H 2 O 2 ) and 2,2-diphenyl-1-picrylhydrazyl (DPPH ) radicals and inhibition of lipid peroxidation. GC–MS analysis of essential oil revealed the presence of 43 components constituting 99.2% of oil. The oil was monoterpenoid (94.35% of oil) with citronellal (60.66%), β -citronellol (12.58%) and isopulegol (8.19%) as the major monoterpenoids. Oil and its major monoterpenes exhibited moderate to strong antioxidant activity in terms of TAA, FRAP and Fe +2 chelating, DPPH and H 2 O 2 scavenging, and lipid peroxidation inhibition. The study concludes that E. citriodora leaves contain monoterpenoid rich oil exhibiting antioxidant activity.
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chemical composition and antioxidant activity of essential oil from residues of artemisia scoparia
Food Chemistry, 2009Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, Sunil Mittal, R K KohliAbstract:Abstract The chemical composition of hydrodistilled oil (yield ∼0.17%, w/v), from the residues of Artemisia scoparia Waldst. & Kit. (sagebrush or wormweed), was analysed for the first time by GC/GC–MS. Of the 49 compounds present in the oil, 48, accounting for 99.28% of the oil, were identified. The volatile oil contained 24 monoterpenoids (56.7%), 19 sesquiterpenoids (28.7%), 2 ketones (0.25%), 1 ester (1.87%), 1 chromene (precocene II, 0.65%) and a hydrocarbon compound. Citronellal (15.2%) followed by β-citronellol (11%) were the major monoterpene constituents of the oil. The residue essential oils (25–200 μg/ml) exhibited a strong antioxidant and radical scavenging activity against hydroxyl ion (OH ) and hydrogen peroxide (H 2 O 2 ). This study concludes that residues of A. scoparia could serve as an important bioresource for extraction of monoterpenoid-rich oil exhibiting antioxidant activity, and thus hold a good potential for use in the food and pharmaceutical industry.
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chemical composition and phytotoxicity of volatile essential oil from intact and fallen leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A total of 23 volatile constituents was identified and characterized by GC and GC-MS in the volatile essential oil extracted from intact (juvenile and adult) and fallen (senescent and leaf litter) leaves of lemon-scented eucalyptus (Eucalyptus citriodora Hook.). The leaves differed in their pigment, water and protein content, and C/N ratio. The oils were, in general, monoterpenoid in nature with 18 monoterpenes and 5 sesquiterpenes. However, a great variability in the amount of essential oils and their individual constituents was observed in different leaf tissues. The amount was maximum in the senescent leaves collected from the floor of the tree closely followed by that from juvenile leaves. In all, 19 constituents were identified in oil from juvenile and senescent leaves compared to 23 in adult leaves and 20 in leaf litter, respectively. Citronellal, a characteristic monoterpene of the oil reported hitherto was found to be more (77-78%) in the juvenile and senescent leaves compared to 48 and 54%, respectively, in the adult leaves and leaf litter. In the adult leaves, however, the content of citronellol--another important monoterpene-- was very high (21.9%) compared to other leaf types (7.8-12.2%). Essential oil and its two major monoterpenes viz. citronellal and citronellol were tested for their phytotoxicity against two weeds (Amaranthus viridis and Echinochloa crus-galli) and two crops (Triticum aestivum and Oryza sativa) under laboratory conditions. A difference in the phytotoxicity, measured in terms of seedling length and dry weight, of oil from different leaves and major monoterpenes was observed. Oil from adult leaves was found to be most phytotoxic although it occurs in smaller amount (on unit weight basis). The different toxicity of different oil types was due to the relative amount of individual monoterpenes present in the oil, their solubility and interactive action. The study concludes that oil from senescent and juvenile leaves being rich in citronellal could be used as commercial source of citronellal whereas that from adult leaves for weed management programmes as it was the most phytotoxic.
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chemical composition and inhibitory activity of essential oil from decaying leaves of eucalyptus citriodora
Zeitschrift für Naturforschung C, 2006Co-Authors: Daizy R. Batish, Shalinder Kaur, Harminder Pal Singh, N Setia, R K KohliAbstract:A study was undertaken to explore the content and composition of volatile oil from decaying leaves of lemon-scented eucalypt (Eucalyptus citriodora Hook.) not analyzed earlier. GC and GC-MS analysis of the oil (yield 0.6%) revealed the monoterpenoid nature with citronellal (52.2%), citronellol (12.3%) and isoisopulegol (11.9%) as the major constituents. Overall, 17 components were identified that accounted for over 94% of the decaying leaf oil. Surprisingly, the decaying leaf oil contained nearly 1.8% of trans-rose oxide, which is generally absent in eucalypt essential oil. Decaying leaf oil and its major 2 components (citronellal and citronellol) inhibited the germination and root elongation of two weeds--Cassia occidentalis (broad-leaved) and Echinochloa crus-galli (grassy weed). Based on the dose-response studies, I50 values were determined for decaying leaf oil and the effect was more on germination only of broad-leaved weed (C. occidentalis), whereas that of citronellal and citronellol were on germination as well as root length of E. crus-galli (grassy weed). Based on I50 values it was observed that citronellal was more phytotoxic and germination inhibiting in nature, whereas citronellol was a more potent root inhibitor, thereby indicating a possible different mode of action. The study concludes that decaying leaf oil hold a good commercial value for exploitation as weed management agent.
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comparative phytotoxicity of four monoterpenes against cassia occidentalis
Annals of Applied Biology, 2002Co-Authors: Harminder Pal Singh, Shalinder Kaur, Daizy R. Batish, H Ramezani, R K KohliAbstract:The effect of four monoterpenes - citronellol, citronellal, cineole and linalool - on the germination, growth and physiology of Cassia occidentalis was investigated. All four monoterpenes reduced germination of C. occidentalis seeds but to varying extents. Citronellal and linalool completely inhibited germination beyond the concentrations of 55 and 110 μM, respectively, whereas in response to treatment of citronellol no germination was observed beyond 330 μM. Further, the growth of seedlings, measured in terms of seedling length and biomass, was also adversely affected. A reduction in chlorophyll content of the cotyledonary leaves of C. occidentalis was also noticed, indicating an adverse effect on photosynthesis. Likewise, respiratory ability of growing seeds was also impaired in response to all four monoterpenes, clearly indicating that monoterpenes affect energy metabolism. On the basis of overall phytotoxicity, potency of monoterpenes was in the order of citronellal > linalool > citronellol > cineole. The results from this study suggest that both citronellal and linalool possess strong phytotoxic potential and can thus serve as lead molecules for the synthesis of bioherbicides.
