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Cristina A Figueiredo - One of the best experts on this subject based on the ideXlab platform.
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bioactivity of ruta graveolens and Satureja montana essential oils on solanum tuberosum hairy roots and solanum tuberosum hairy roots with meloidogyne chitwoodi co cultures
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Jorge M S Faria, Ana Rodrigues, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:As a nematotoxics screening biotechnological system, Solanum tuberosum hairy roots (StHR) and S. tuberosum hairy roots with Meloidogyne chitwoodi co-cultures (StHR/CRKN) were evaluated, with and without the addition of the essential oils (EOs) of Satureja montana and Ruta graveolens. EOs nematotoxic and phytotoxic effects were followed weekly by evaluating nematode population density in the co-cultures as well as growth and volatile profiles of both in vitro cultures types. Growth, measured by the dissimilation method and by fresh and dry weight determination, was inhibited after EO addition. Nematode population increased in control cultures, while in EO-added cultures numbers were kept stable. In addition to each of the EOs main components, and in vitro cultures constitutive volatiles, new volatiles were detected by gas chromatography and gas chromatography coupled to mass spectrometry in both culture types. StHR with CRKN co-cultures showed to be suitable for preliminary assessment of nematotoxic EOs.
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nematotoxic and phytotoxic activity of Satureja montana and ruta graveolens essential oils on pinus pinaster shoot cultures and p pinaster with bursaphelenchus xylophilus in vitro co cultures
Industrial Crops and Products, 2015Co-Authors: Jorge M S Faria, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:Abstract Satureja montana (winter savory) and Ruta graveolens (rue) nematotoxic essential oils (EOs) (0.5 μl EO/ml culture medium) were assessed for the first time in Pinus pinaster in vitro shoot cultures (Ppi) and P . pinaster shoots with Bursaphelenchus xylophilus co-cultures (PpiBx). The EOs nematotoxic effect was evaluated on B . xylophilus population density in PpiBx co-cultures and the phytotoxic activity to the host was assessed by evaluating relative water content and volatile profiles both on Ppi cultures and on PpiBx co-cultures. Carvacrol-rich S . montana EO showed phytotoxicity, by inducing shoot chlorosis and drooping, whereas no major morphological changes were detected on R . graveolens EO-added Ppi and PpiBx in vitro cultures. Both EOs maintained the nematotoxicity during all experimental phases. R . graveolens EO proved to be an effective PWN antagonist to be further evaluated for pine wilt disease control, given its less phytotoxicity while maintaining nematoxicity.
Valerija Dunkić - One of the best experts on this subject based on the ideXlab platform.
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Glycosidically Bound Volatile Compounds of Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and Endemic S. visianii silic
Advances in Biological Chemistry, 2015Co-Authors: Valerija Dunkić, Nada Bezić, Ivana Radovanović, Elma VukoAbstract:In this paper, the glycoconjugated volatile compounds of four Croatian Satureja species (Satureja montana L., S. cuneifolia Ten., S. subspicata Vis. and endemic S. visianii Silic) were investigated. Content and composition of these compounds were examined depending on the stage of plant development. GC and GC–MS analysis of volatile aglycones revealed twenty-one compounds. Thymoquinone, geraniol and carvacrol were detected in all vegetative phases of the investigated plants. Other quantitatively important aglycones were eugenol and thymol of S. montana, phenyl ethyl alcohol, benzene acetaldehyde, borneol, α-terpineol, thymol and eugenol of S. cuneifolia, phenyl ethyl alcohol, benzene acetaldehyde, terpinen-4-ol, α-terpineol and β-ionone of S. subspicata and camphor, thymol and 8a-acetoxylemolol of S. visianii. Moderate similarity in the chemical composition of essential oils and volatile aglycones of investigated plant species indicate that many biologically active compounds are glycosylated and accumulate as non-volatile glycosides.
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Chemotaxonomic and Micromorphological Traits of Satureja montana L. and S. subspicata Vis. (Lamiaceae)
Chemistry & biodiversity, 2012Co-Authors: Valerija Dunkić, Dario Kremer, Ivna Dragojević Müller, Edith Stabentheiner, Sunčica Kuzmić, Renata Jurišić Grubešić, Lovorka Vujić, Ivan Kosalec, Marko Randić, Siniša SrečecAbstract:Satureja montana and S. subspicata are used as spice, pepper substitute, for preparing tea, juice, and as a medicine. Fourteen populations (seven per species) of Satureja montana L. and S. subspicata Vis. growing in Croatia were examined to determine the chemical composition of the essential oil (analyzed by GC-FID and GC/MS), the content of macroelements (Na, K, Ca, Mg) and trace elements (B, Fe, Cu, Mn, Zn, Al, Pb, Cr, Cd, Ni, Hg, As) analyzed by ICP-AES, antioxidant compounds (analyzed by UV/VIS spectrophotometer), and the types and distribution of trichomes (analyzed by scanning electron microscopy). The main constituents of the essential oil were carvacrol and thymol in S. montana (all populations belong to one phenol chemotype), while α-eudesmol, β-eudesmol, and spathulenol dominated in S. subspicata (three chemotypes could be distinguished). Both species possess considerably higher quantities of Ca and Mg, and moderate concentrations of K and Na, while Hg and As levels were below the limit of quantification. Non-glandular trichomes, peltate trichomes, and three types of capitate trichomes were observed on leaves, stem, calyx, and corolla.
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Antiphytoviral Activity of Satureja montana L. ssp. variegata (Host) P. W. Ball Essential Oil and Phenol Compounds on CMV and TMV
Molecules, 2010Co-Authors: Valerija Dunkić, Nada Bezić, Elma Vuko, Dubravka CukrovAbstract:The essential oil of Satureja montana L. ssp. variegata (Host) P. W. Ball (Lamiaceae) was characterized by a high concentration of oxygenated monoterpenes (71.3%), among which carvacrol (19.4%) and thymol (16.6%) were the major compounds. When the essential oil was applied on local hosts Chenopodium amaranticolor Coste & Reyn. and Chenopodium quinoa Willd. simultaneously with the infecting virus, the number of local lesions on both Tobacco mosaic virus (TMV) and Cucumber mosaic virus (CMV) infected plants was reduced for 29.2% and 24.1%, respectively. When applied individually for each virus, thymol was more effective in reducing CMV infection (33.2%), while carvacrol was more effective in reducing the TMV infection (34.3%). No synergistic effect of both monoterpenes was observed in the antiviral activity of the oil.
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Phytochemical composition and antimicrobial activity of Satureja montana L. and Satureja cuneifolia Ten. essential oils
Acta Botanica Croatica, 2005Co-Authors: Nada Bezić, Mirjana Skočibušić, Valerija DunkićAbstract:The phytochemical composition and the antibacterial activity of the essential oils obtained from the aerial parts of two Lamiaceae species, winter savory (Satureja montana L.) and wild savory (Satureja cuneifolia Ten.) were evaluated. Gas chromatography-mass spectrometry (GC-MS) analysis of the isolated oils resulted in the identification of twenty compounds in the oil of S. montana representing 97% of the total oil and 25 compounds of S. cuneifolia, representing 80% of the total oil. Carvacrol was the major constituent of the S. montana oil (45.7%). Other important compounds were the monoterpenic hydrocarbons p-cymene, -terpinene and the oxygenated compounds carvacrol methyl ether, borneol and thymol. Conversely, the oil of S. cuneifolia contained a low percentage of carvacrol and thymol. The major constituents of wild savory oil were sesquiterpenes -cubebene (8.7%), spathulenol, -caryophyllene, followed by the monoterpenic hydrocarbons limonene and -pinene. The screening of the antimicrobial activities of essential oils were individually evalated against nine microorganisms, using a disc diffusion metod. The oil of S. montana exhibited greater antimicrobial activity than the oil of wild savory. Maximum activity of winter savory oil was observed against Escherichia coli, the methicillin-resistant Staphylococcus aureus and against yeast (Candida albicans). The essential oil of S. cuneifolia was also found to inhibit the growth of pathogens such as S. aureus and E. coli. A fungicidal activity against C. albicans and Saccharomyces cerevisiae was also found in both oils.
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Cytotoxic and mutagenic in vitro effect of the Satureja montana L. essential oils
2002Co-Authors: Mirjana Skočibušić, Nada Bezić, Valerija DunkićAbstract:The essential oils have been widely used as fragrances in cosmetics, as flavouring food additives, as scenting agents in a variety of household products, and as active ingredients in some old drugs. The genus Satureja (Lamiaceae) is represented in Croatia by nine species and among them Winter Savory (Satureja montana L.) is economically the most important. Essential oils from investigated species have several biological activities which include antibacterial, antifungal and antiviral effect. Volatile oils are very complex mixture of compounds. The biological activity is the result of the combined effects of both their active and inactive componds. Those molecules are formed in glandular cells by various pathways. A large number of oils have a moderate irritant risk, including the essential oils of S. montana. The genotoxicity data show that frequently used herbs may also be strongs mutagens and thus potential carcinogens. Genotoxic potental of investigated essential oils is unknow. The aim of the present study is the evaluation of cytotoxic and mutagenic effect of the essential oils extracted from Winter Savory. The volatile components of the investigated species have been analysed using GC and GC-MS. Essential oils of S. montana showed high content of carvacrol (45, 7%) and p-cymene (12, 6%). The in vitro genotoxicity of essential oils was assessed in short-term tests systems, in the Salmonella thyphimurium mutagenicity assay and oxidative mutagenesis with Escherichia coli. The essential oil of S. montana was not mutagenic in S. thyphimurium strains TA1535 and TA1537 but a weak response was observed in TA98 and TA100 tester strain both without and with metabolic activation (rat liver S9 mix). A negative control corresponding to solvent was also used. Cytotoxic effect is demonstrated by a reduction or elimination of the background lawn without metabolic activation in strains TA98 and TA100 at a concentration of 0, 4 % essential oils, but the effect was reduced when S9 was present. Screening of oxidative mutagenesis with Escherichia coli strains IC188 and IC 203 gave negative results.
Jorge M S Faria - One of the best experts on this subject based on the ideXlab platform.
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bioactivity of ruta graveolens and Satureja montana essential oils on solanum tuberosum hairy roots and solanum tuberosum hairy roots with meloidogyne chitwoodi co cultures
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Jorge M S Faria, Ana Rodrigues, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:As a nematotoxics screening biotechnological system, Solanum tuberosum hairy roots (StHR) and S. tuberosum hairy roots with Meloidogyne chitwoodi co-cultures (StHR/CRKN) were evaluated, with and without the addition of the essential oils (EOs) of Satureja montana and Ruta graveolens. EOs nematotoxic and phytotoxic effects were followed weekly by evaluating nematode population density in the co-cultures as well as growth and volatile profiles of both in vitro cultures types. Growth, measured by the dissimilation method and by fresh and dry weight determination, was inhibited after EO addition. Nematode population increased in control cultures, while in EO-added cultures numbers were kept stable. In addition to each of the EOs main components, and in vitro cultures constitutive volatiles, new volatiles were detected by gas chromatography and gas chromatography coupled to mass spectrometry in both culture types. StHR with CRKN co-cultures showed to be suitable for preliminary assessment of nematotoxic EOs.
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nematotoxic and phytotoxic activity of Satureja montana and ruta graveolens essential oils on pinus pinaster shoot cultures and p pinaster with bursaphelenchus xylophilus in vitro co cultures
Industrial Crops and Products, 2015Co-Authors: Jorge M S Faria, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:Abstract Satureja montana (winter savory) and Ruta graveolens (rue) nematotoxic essential oils (EOs) (0.5 μl EO/ml culture medium) were assessed for the first time in Pinus pinaster in vitro shoot cultures (Ppi) and P . pinaster shoots with Bursaphelenchus xylophilus co-cultures (PpiBx). The EOs nematotoxic effect was evaluated on B . xylophilus population density in PpiBx co-cultures and the phytotoxic activity to the host was assessed by evaluating relative water content and volatile profiles both on Ppi cultures and on PpiBx co-cultures. Carvacrol-rich S . montana EO showed phytotoxicity, by inducing shoot chlorosis and drooping, whereas no major morphological changes were detected on R . graveolens EO-added Ppi and PpiBx in vitro cultures. Both EOs maintained the nematotoxicity during all experimental phases. R . graveolens EO proved to be an effective PWN antagonist to be further evaluated for pine wilt disease control, given its less phytotoxicity while maintaining nematoxicity.
José S. Urieta - One of the best experts on this subject based on the ideXlab platform.
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Phytochemical profile and anticholinesterase and antimicrobial activities of supercritical versus conventional extracts of Satureja montana.
Journal of agricultural and food chemistry, 2009Co-Authors: Filipa V.m. Silva, Nuno R. Neng, José M.f. Nogueira, Clara Grosso, Alice Martins, Joana Salta, Delfina Mira, Natália Gaspar, Jorge Justino, José S. UrietaAbstract:Winter savory Satureja montana is a medicinal herb used in traditional gastronomy for seasoning meats and salads. This study reports a comparison between conventional (hydrodistillation, HD, and Soxhlet extraction, SE) and alternative (supercritical fluid extraction, SFE) extraction methods to assess the best option to obtain bioactive compounds. Two different types of extracts were tested, the volatile (SFE-90 bar, second separator vs HD) and the nonvolatile fractions (SFE-250 bar, first and second separator vs SE). The inhibitory activity over acetyl- and butyrylcholinesterase by S. montana extracts was assessed as a potential indicator for the control of Alzheimer's disease. The supercritical nonvolatile fractions, which showed the highest content of (+)-catechin, chlorogenic, vanillic, and protocatechuic acids, also inhibited selectively and significantly butyrylcholinesterase, whereas the nonvolatile conventional extract did not affect this enzyme. Microbial susceptibility tests revealed the great potential of S. montana volatile supercritical fluid extract for the growth control and inactivation of Bacillus subtilis and Bacillus cereus, showing some activity against Botrytis spp. and Pyricularia oryzae. Although some studies were carried out on S. montana, the phytochemical analysis together with the biological properties, namely, the anticholinesterase and antimicrobial activities of the plant nonvolatile and volatile supercritical fluid extracts, are described herein for the first time.
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Antioxidant activities of the supercritical and conventional Satureja montana extracts.
Journal of food science, 2009Co-Authors: Clara Grosso, Ariclécio Cunha De Oliveira, Ana M. Mainar, José S. Urieta, José G. Barroso, António F. PalavraAbstract:The antioxidant activities of the volatile and the nonvolatile fractions from Satureja montana obtained by supercritical fluid extraction (SFE) and by conventional techniques, hydrodistillation (HD) and soxhlet extraction (SE), were compared. A good agreement between DPPH and rancimat methods was obtained showing that the extracts were able to scavenge free radicals and to inhibit lipid oxidation. The volatile oil (obtained by SFE at 90 bar/40 degrees C) was the most effective extract, presenting the lowest EC(50) (0.06 g/L) and the highest protector factor (PF = 2.03). These results demonstrated the advantages of SFE over conventional techniques by avoiding thermodegradation and hydrolysis reactions. Furthermore, volatile oil is 15 times richer in thymoquinone than the essential oil (HD). This compound is of great importance due to its antioxidant, neuroprotective, and anti-cancer activities. The combination of carvacrol + thymol + thymoquinone in volatile oil may be responsible for the increase in the antioxidant activity when compared to HD, which demonstrates that, in this case, SFE improves value to the final product.
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Enrichment of the thymoquinone content in volatile oil from Satureja montana using supercritical fluid extraction.
Journal of separation science, 2009Co-Authors: Clara Grosso, Ana M. Mainar, José S. Urieta, José G. Barroso, Ana Cristina Figueiredo, Jesus Burillo, José A. Coelho, António F. PalavraAbstract:Supercritical fluid extraction (SFE) of the volatile oil from Satureja montana L. was performed under different conditions of pressure (90 and 100 bar), temperature (40 and 50 degrees C), mean particle sizes (0.4, 0.6 and 0.8 mm) and CO(2) flow rate (0.8, 1.1 and 1.3 kg/h) to understand the influence of these parameters on the composition and yield of this oil. The results were compared with those obtained for the essential oil isolated by hydrodistillation (HD). The volatile and the essential oil were analysed by GC and GC-MS. The main compounds are carvacrol (52.2-62.0% for HD vs. 41.7-64.5% for SFE), thymol (8.6-11.0% for HD vs. 6.0-11.3% for SFE), p-cymene (6.9-12.8% for HD vs. 6.0-17.8% for SFE), gamma-terpinene (6.4-9.4% for HD vs. 2.3-6.0% for SFE) and beta-bisabolene (2.0-2.7% for HD vs. 2.2-3.5% for SFE). The major difference between SFE and HD was the relative amount of thymoquinone, an oxygenated monoterpene with important biological activities, which can be ten-fold higher in volatile oil (1.6-3.0 for SFE vs. 0.2% for HD). The morphology of the glandular trichomes of S. montana and the effect of the grinding process on them was also evaluated by SEM.
M Mota - One of the best experts on this subject based on the ideXlab platform.
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bioactivity of ruta graveolens and Satureja montana essential oils on solanum tuberosum hairy roots and solanum tuberosum hairy roots with meloidogyne chitwoodi co cultures
Journal of Agricultural and Food Chemistry, 2016Co-Authors: Jorge M S Faria, Ana Rodrigues, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:As a nematotoxics screening biotechnological system, Solanum tuberosum hairy roots (StHR) and S. tuberosum hairy roots with Meloidogyne chitwoodi co-cultures (StHR/CRKN) were evaluated, with and without the addition of the essential oils (EOs) of Satureja montana and Ruta graveolens. EOs nematotoxic and phytotoxic effects were followed weekly by evaluating nematode population density in the co-cultures as well as growth and volatile profiles of both in vitro cultures types. Growth, measured by the dissimilation method and by fresh and dry weight determination, was inhibited after EO addition. Nematode population increased in control cultures, while in EO-added cultures numbers were kept stable. In addition to each of the EOs main components, and in vitro cultures constitutive volatiles, new volatiles were detected by gas chromatography and gas chromatography coupled to mass spectrometry in both culture types. StHR with CRKN co-cultures showed to be suitable for preliminary assessment of nematotoxic EOs.
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nematotoxic and phytotoxic activity of Satureja montana and ruta graveolens essential oils on pinus pinaster shoot cultures and p pinaster with bursaphelenchus xylophilus in vitro co cultures
Industrial Crops and Products, 2015Co-Authors: Jorge M S Faria, Ines Sena, Cristina Moiteiro, Richard N Bennett, M Mota, Cristina A FigueiredoAbstract:Abstract Satureja montana (winter savory) and Ruta graveolens (rue) nematotoxic essential oils (EOs) (0.5 μl EO/ml culture medium) were assessed for the first time in Pinus pinaster in vitro shoot cultures (Ppi) and P . pinaster shoots with Bursaphelenchus xylophilus co-cultures (PpiBx). The EOs nematotoxic effect was evaluated on B . xylophilus population density in PpiBx co-cultures and the phytotoxic activity to the host was assessed by evaluating relative water content and volatile profiles both on Ppi cultures and on PpiBx co-cultures. Carvacrol-rich S . montana EO showed phytotoxicity, by inducing shoot chlorosis and drooping, whereas no major morphological changes were detected on R . graveolens EO-added Ppi and PpiBx in vitro cultures. Both EOs maintained the nematotoxicity during all experimental phases. R . graveolens EO proved to be an effective PWN antagonist to be further evaluated for pine wilt disease control, given its less phytotoxicity while maintaining nematoxicity.
