Scaffold Interaction

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A T Brini - One of the best experts on this subject based on the ideXlab platform.

  • human adipose derived stem cells isolated from young and elderly women their differentiation potential and Scaffold Interaction during in vitro osteoblastic differentiation
    Cytotherapy, 2009
    Co-Authors: L De Girolamo, M F Sartori, E Arrigoni, S Lopa, Franz Baruffaldi W Preis, A T Brini
    Abstract:

    BACKGROUND AIMS: Several authors have demonstrated that adipose tissue contains multipotent cells capable of differentiation into several lineages, including bone, cartilage and fat. METHODS: This study compared human adipose-derived stem cells (hASC) isolated from 26 female donors, under 35 and over 45 years old, showing differences in their cell numbers and proliferation, and evaluated their in vitro adipocytic and osteoblastic differentiation potential. RESULTS: The cellular yield of hASC from older donors was significantly greater than that from younger donors, whereas their clonogenic potential appeared slightly reduced. There were no significant discrepancies between hASC isolated from young and elderly women regarding their in vitro adipocytic differentiation, whereas the osteoblastic potential was significantly reduced by aging. We also assessed the influence of hydroxyapatite (HAP) and silicon carbide (SiC-PECVD) on hASC. Even when cultured on Scaffolds, hASC from younger donors had better differentiation into osteoblast-like cells than hASC from older donors; their differentiation ability was up-regulated by the presence of HAP, whereas SiC-PECVD produced no significant effect on hASC osteoblastic differentiation. CONCLUSIONS: The large numbers of hASC resident in adipose tissue and their differentiation features suggest that they could be used for a successful bone regeneration process in vivo. We have shown that age does not seem to affect cell viability and in vitro adipocytic differentiation significantly, whereas it does affects osteoblastic differentiation, in the absence and presence of two-dimensional and three-dimensional Scaffolds.

  • human adipose derived stem cells as future tools in tissue regeneration osteogenic differentiation and cell Scaffold Interaction
    International Journal of Artificial Organs, 2008
    Co-Authors: L De Girolamo, M F Sartori, E Arrigoni, Lia Rimondini, W Albisetti, R Weinstein, A T Brini
    Abstract:

    Tissue engineering is now contributing to new developments in several clinical fields, and mesenchymal stem cells derived from adipose tissue (hASCs) may provide a novel opportunity to replace, repair and promote the regeneration of diseased or damaged musculoskeletal tissue. Our interest was to characterize and differentiate hASCs isolated from twenty-three donors. Proliferation, CFU-F, cytofluorimetric and histochemistry analyses were performed. HASCs differentiate into os- teogenic, chondrogenic, and adipogenic lineages, as assessed by tissue-specific markers such as alkaline phosphatase, osteopontin expression and deposition of calcium matrix, lipid-vacuoles for- mation and Glycosaminoglycans production. We also compared osteo-differentiated hASCs cultured on monolayer and loaded on biomaterials routinely used in the clinic, such as hydroxyapatite, can- cellous human bone fragments, deproteinized bovine bone granules, and titanium. Scaffolds loaded with pre-differentiated hASCs do not affect cell proliferation and no cellular toxicity was observed. HASCs tightly adhere to Scaffolds and differentiated-hASCs on human bone fragments and bovine bone granules produced, respectively, 3.4- and 2.1-fold more calcified matrix than osteo-differentiat- ed hASCs on monolayer. Moreover, both human and deproteinized bovine bone is able to induce os- teogenic differentiation of CTRL-hASCs. Although our in vitro results need to be confirmed in in vivo bone regeneration models, our data suggest that hASCs may be considered suitable biological tools for the screening of innovative Scaffolds that would be useful in tissue engineering. (Int J Artif Organs 2008; 31: )

Ayyoob Arpanaei - One of the best experts on this subject based on the ideXlab platform.

L De Girolamo - One of the best experts on this subject based on the ideXlab platform.

  • Human adipose-derived stem cells isolated from young and elderly women: Their differentiation potential and Scaffold Interaction during in vitro osteoblastic differentiation
    Cytotherapy, 2020
    Co-Authors: L De Girolamo, M F Sartori, S Lopa, Franz Baruffaldi W Preis, Elena Arrigoni, Anna T. Brini
    Abstract:

    Background aimsSeveral authors have demonstrated that adipose tissue contains multipotent cells capable of differentiation into several lineages, including bone, cartilage and fat.MethodsThis study compared human adipose-derived stem cells (hASC) isolated from 26 female donors, under 35 and over 45 years old, showing differences in their cell numbers and proliferation, and evaluated their in vitro adipocytic and osteoblastic differentiation potential.ResultsThe cellular yield of hASC from older donors was significantly greater than that from younger donors, whereas their clonogenic potential appeared slightly reduced. There were no significant discrepancies between hASC isolated from young and elderly women regarding their in vitro adipocytic differentiation, whereas the osteoblastic potential was significantly reduced by aging. We also assessed the influence of hydroxyapatite (HAP) and silicon carbide (SiC–PECVD) on hASC. Even when cultured on Scaffolds, hASC from younger donors had better differentiatio...

  • human adipose derived stem cells isolated from young and elderly women their differentiation potential and Scaffold Interaction during in vitro osteoblastic differentiation
    Cytotherapy, 2009
    Co-Authors: L De Girolamo, M F Sartori, E Arrigoni, S Lopa, Franz Baruffaldi W Preis, A T Brini
    Abstract:

    BACKGROUND AIMS: Several authors have demonstrated that adipose tissue contains multipotent cells capable of differentiation into several lineages, including bone, cartilage and fat. METHODS: This study compared human adipose-derived stem cells (hASC) isolated from 26 female donors, under 35 and over 45 years old, showing differences in their cell numbers and proliferation, and evaluated their in vitro adipocytic and osteoblastic differentiation potential. RESULTS: The cellular yield of hASC from older donors was significantly greater than that from younger donors, whereas their clonogenic potential appeared slightly reduced. There were no significant discrepancies between hASC isolated from young and elderly women regarding their in vitro adipocytic differentiation, whereas the osteoblastic potential was significantly reduced by aging. We also assessed the influence of hydroxyapatite (HAP) and silicon carbide (SiC-PECVD) on hASC. Even when cultured on Scaffolds, hASC from younger donors had better differentiation into osteoblast-like cells than hASC from older donors; their differentiation ability was up-regulated by the presence of HAP, whereas SiC-PECVD produced no significant effect on hASC osteoblastic differentiation. CONCLUSIONS: The large numbers of hASC resident in adipose tissue and their differentiation features suggest that they could be used for a successful bone regeneration process in vivo. We have shown that age does not seem to affect cell viability and in vitro adipocytic differentiation significantly, whereas it does affects osteoblastic differentiation, in the absence and presence of two-dimensional and three-dimensional Scaffolds.

  • human adipose derived stem cells as future tools in tissue regeneration osteogenic differentiation and cell Scaffold Interaction
    International Journal of Artificial Organs, 2008
    Co-Authors: L De Girolamo, M F Sartori, E Arrigoni, Lia Rimondini, W Albisetti, R Weinstein, A T Brini
    Abstract:

    Tissue engineering is now contributing to new developments in several clinical fields, and mesenchymal stem cells derived from adipose tissue (hASCs) may provide a novel opportunity to replace, repair and promote the regeneration of diseased or damaged musculoskeletal tissue. Our interest was to characterize and differentiate hASCs isolated from twenty-three donors. Proliferation, CFU-F, cytofluorimetric and histochemistry analyses were performed. HASCs differentiate into os- teogenic, chondrogenic, and adipogenic lineages, as assessed by tissue-specific markers such as alkaline phosphatase, osteopontin expression and deposition of calcium matrix, lipid-vacuoles for- mation and Glycosaminoglycans production. We also compared osteo-differentiated hASCs cultured on monolayer and loaded on biomaterials routinely used in the clinic, such as hydroxyapatite, can- cellous human bone fragments, deproteinized bovine bone granules, and titanium. Scaffolds loaded with pre-differentiated hASCs do not affect cell proliferation and no cellular toxicity was observed. HASCs tightly adhere to Scaffolds and differentiated-hASCs on human bone fragments and bovine bone granules produced, respectively, 3.4- and 2.1-fold more calcified matrix than osteo-differentiat- ed hASCs on monolayer. Moreover, both human and deproteinized bovine bone is able to induce os- teogenic differentiation of CTRL-hASCs. Although our in vitro results need to be confirmed in in vivo bone regeneration models, our data suggest that hASCs may be considered suitable biological tools for the screening of innovative Scaffolds that would be useful in tissue engineering. (Int J Artif Organs 2008; 31: )

Xiaojun Zhao - One of the best experts on this subject based on the ideXlab platform.

  • a 3d model of ovarian cancer cell lines on peptide nanofiber Scaffold to explore the cell Scaffold Interaction and chemotherapeutic resistance of anticancer drugs
    International Journal of Nanomedicine, 2011
    Co-Authors: Zehong Yang, Xiaojun Zhao
    Abstract:

    RADA16-I peptide hydrogel, a type of nanofiber Scaffold derived from self-assembling peptide RADA16-I, has been extensively applied to regenerative medicine and tissue repair in order to develop novel nanomedicine systems. In this study, using RADA16-I peptide hydrogel, a three-dimensional (3D) cell culture model was fabricated for in vitro culture of three ovarian cancer cell lines. Firstly, the peptide nanofiber Scaffold was evaluated by transmission electron microscopy and atom force microscopy. Using phase contrast microscopy, the appearance of the representative ovarian cancer cells encapsulated in RADA16-I peptide hydrogel on days 1, 3, and 7 in 24-well Petri dishes was illustrated. The cancer cell–nanofiber Scaffold construct was cultured for 5 days, and the ovarian cancer cells had actively proliferative potential. The precultured ovarian cancer cells exhibited nearly similar adhesion properties and invasion potentials in vitro between RADA16-I peptide nanofiber and type I collagen, which suggested that RADA16-I peptide hydrogel had some similar characteristics to type I collagen. The precultured ovarian cancer cells had two-fold to five-fold higher anticancer drug resistance than the conventional two-dimensional Petri dish culture. So the 3D cell model on peptide nanofiber Scaffold is an optimal type of cell pattern for anticancer drug screening and tumor biology.

  • A 3D model of ovarian cancer cell lines on peptide nanofiber Scaffold to explore the cell–Scaffold Interaction and chemotherapeutic resistance of anticancer drugs
    International Journal of Nanomedicine, 2011
    Co-Authors: Zehong Yang, Xiaojun Zhao
    Abstract:

    RADA16-I peptide hydrogel, a type of nanofiber Scaffold derived from self-assembling peptide RADA16-I, has been extensively applied to regenerative medicine and tissue repair in order to develop novel nanomedicine systems. In this study, using RADA16-I peptide hydrogel, a three-dimensional (3D) cell culture model was fabricated for in vitro culture of three ovarian cancer cell lines. Firstly, the peptide nanofiber Scaffold was evaluated by transmission electron microscopy and atom force microscopy. Using phase contrast microscopy, the appearance of the representative ovarian cancer cells encapsulated in RADA16-I peptide hydrogel on days 1, 3, and 7 in 24-well Petri dishes was illustrated. The cancer cell–nanofiber Scaffold construct was cultured for 5 days, and the ovarian cancer cells had actively proliferative potential. The precultured ovarian cancer cells exhibited nearly similar adhesion properties and invasion potentials in vitro between RADA16-I peptide nanofiber and type I collagen, which suggested that RADA16-I peptide hydrogel had some similar characteristics to type I collagen. The precultured ovarian cancer cells had two-fold to five-fold higher anticancer drug resistance than the conventional two-dimensional Petri dish culture. So the 3D cell model on peptide nanofiber Scaffold is an optimal type of cell pattern for anticancer drug screening and tumor biology.

Fatemeh Ajalloueian - One of the best experts on this subject based on the ideXlab platform.

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