The Experts below are selected from a list of 10233 Experts worldwide ranked by ideXlab platform

Bei Zhang - One of the best experts on this subject based on the ideXlab platform.

  • Saturated fatty acids activate the inflammatory signalling pathway in Schwann cells: Implication in Sciatic Nerve Injury.
    Scandinavian journal of immunology, 2020
    Co-Authors: Jie Liang, Mengli Cui, Li Zhang, Shurong Ren, Wenxiang Zheng, Xiaolei Dong, Bei Zhang
    Abstract:

    Sciatic Nerve Injury affects quality of life. Many immune cells and inflammatory cytokines have been reported to be involved in Sciatic Nerve Injury, but little is known about the ligands and receptors that trigger inflammatory responses. By using a modified Sciatic Nerve clamp Injury method, we found that the recruitment of Schwann cells and the inflammatory response were enhanced after Sciatic Nerve Injury. Toll-like receptor 4 (TLR4), one of the major members of the TLR family, is highly expressed in Schwann cells. Under certain conditions, myeloid differentiation protein 2 (MD2) binds to TLR4 on the membrane and plays important roles in the inflammatory response. The reductions in the recruitment of Schwann cells and the inflammatory response induced by the blockade of TLR4 or MD2 suggest that TLR4 and MD2 are involved in Sciatic Nerve Injury. What are the endogenous signals that activate the inflammatory response? A large number of free saturated fatty acids (SFAs) are released from Schwann cells, adipocytes and the blood after Sciatic Nerve Injury. Liang et al reported that Schwann cells can be stimulated by palmitic acid (PA). Here, we found that the expression and secretion of TNF-α and IL-6 were enhanced by PA treatment. Moreover, PA activated TLR4 signalling pathway-related proteins and stimulated a strong association between TLR4 and MD2. Blocking TLR4 or MD2 reversed the PA-induced inflammatory response and TLR4 downstream signalling pathway. Thus, we speculated that SFAs act as endogenous ligands that activate TLR4/MD2, thus triggering Schwann cell inflammation during Sciatic Nerve Injury.

  • The Role of NLRP3 in Repairing Sciatic Nerve Injury
    International journal of sciences, 2019
    Co-Authors: Zhu Yuzhen, Xiao, Jianzhao Deng, Xuan Yang, Bei Zhang
    Abstract:

    NLRP3 is a member of the NOD-like family of adaptive immune responses that recognize both exogenous damage-associated molecular patterns and endogenous damage-related molecular patterns. NLRP3 recognizes that PAMPS/DAMPS is activated, binds to ASC and Caspase-1 to form NLRP3 inflammatory vesicles, and exerts immunity in pathogenic infectious diseases and autoimmune diseases by controlling the release of IL-1β and IL-18, but The role of peripheral Nerve Injury is not clear. The objective of this study is to investigate the effect of NLRP3 on repair after Sciatic Nerve Injury. This study was divided into two parts. The first part established the Sciatic Nerve Injury model. In the second part, NLRP3 intervention was given on the basis of the Sciatic Nerve Injury model. The results showed that The model of Sciatic Nerve Injury in mice was established successfully. The Sciatic Nerve function of the MSU group and the MCC950 group recovered better than the control group, and the recovery of the MCC950 group was the best. It is concluded that moderate inhibition of nlrp3-mediated inflammatory response is helpful for peripheral Nerve repair after Injury.

  • Regional tissue immune responses after Sciatic Nerve Injury in rats
    International journal of clinical and experimental medicine, 2015
    Co-Authors: Yu-ming Chen, Bei Zhang, Ruo-wu Shen, Wei-ning Zhang
    Abstract:

    Inflammatory cells play a critical role during Nerve regeneration following peripheral Nerve Injury. In this study, we investigated immune responses in rat Sciatic Nerve after Injury. Wistar rats were randomly divided into the Sciatic Nerve Injury (model) group and control group. The right Sciatic Nerve of rats in the model group was transected and sutured end-to-end. Our results showed that rats in the model group functionally recovered following Sciatic Nerve Injury. We detected inflammatory cell infiltration in the remaining Sciatic Nerves following Injury. In addition, expression of interferon-γ (INF-γ), interleukin-10 (IL-10), and the INF-γ/IL-10 ratio was significantly elevated one week following Nerve Injury, but gradually decreased thereafter. Our findings demonstrate that immune responses and inflammatory cell activation are involved during recovery from Sciatic Nerve Injury.

  • Original Article Regional tissue immune responses after Sciatic Nerve Injury in rats
    2015
    Co-Authors: Yu-ming Chen, Bei Zhang, Ruo-wu Shen, Wei-ning Zhang
    Abstract:

    Inflammatory cells play a critical role during Nerve regeneration following peripheral Nerve Injury. In this study, we investigated immune responses in rat Sciatic Nerve after Injury. Wistar rats were randomly divided into the Sciatic Nerve Injury (model) group and control group. The right Sciatic Nerve of rats in the model group was transect- ed and sutured end-to-end. Our results showed that rats in the model group functionally recovered following Sciatic Nerve Injury. We detected inflammatory cell infiltration in the remaining Sciatic Nerves following Injury. In addition, expression of interferon-γ (INF-γ), interleukin-10 (IL-10), and the INF-γ/IL-10 ratio was significantly elevated one week following Nerve Injury, but gradually decreased thereafter. Our findings demonstrate that immune responses and inflammatory cell activation are involved during recovery from Sciatic Nerve Injury.

Daniel Offen - One of the best experts on this subject based on the ideXlab platform.

  • Stem cells treatment for Sciatic Nerve Injury
    Expert opinion on biological therapy, 2011
    Co-Authors: Michal Dadon-nachum, Eldad Melamed, Daniel Offen
    Abstract:

    Introduction: Sciatic Nerve Injury is common and usually results in degeneration of the distal axons and muscle denervation. Chronic muscle atrophy and fibrosis limit the recovery of muscle functio...

  • Differentiated Mesenchymal Stem Cells for Sciatic Nerve Injury
    Stem cell reviews and reports, 2011
    Co-Authors: Michal Dadon-nachum, Eldad Melamed, Ofer Sadan, I. Srugo, Daniel Offen
    Abstract:

    Sciatic Nerve Injury is common and may cause neurological deficits. Previous studies showed that administration of neurotrophic factors (NTFs), naturally occurring proteins that support the development and survival of neurons, preserved and protected damaged motor neuron in the injured Sciatic Nerve. We have been successful in converting bone marrow-derived mesenchymal stem cells into astrocyte-like cells that produce and secrete NTFs (NTF(+) cells). These cells demonstrate typical astrocyte morphology, express characteristic astrocyte markers and secrete high levels of NTFs. We have already shown that these cells and their conditioned media can protect neurons in culture and in animal models of neurodegenerative diseases. In the current study we examined whether NTF(+) cells are capable of rescuing motor neurons in a rat Sciatic Nerve Injury model, where the right hind limb Sciatic Nerve was crushed. Rats were transplanted with NTF(+) cells, MSCs or PBS into the lesion site. In rats injected with the NTF(+) cells motor function was markedly preserved. Moreover, NTF(+) cells significantly inhibited the degeneration of the neuromuscular junctions and preserved the myelinated motor axons. Our findings suggest that autologous therapeutic approach can alleviate signs of Sciatic Nerve Injury and probably other neurological disorders.

Hiroshi Ueda - One of the best experts on this subject based on the ideXlab platform.

M A Bisby - One of the best experts on this subject based on the ideXlab platform.

  • increase of preprotachykinin mrna and substance p immunoreactivity in spared dorsal root ganglion neurons following partial Sciatic Nerve Injury
    European Journal of Neuroscience, 1998
    Co-Authors: M A Bisby
    Abstract:

    Complete Sciatic Nerve Injury reduces substance P (SP) expression in primary sensory neurons of the L4 and L5 dorsal root ganglia (DRG), due to loss of target-derived Nerve growth factor (NGF). Partial Nerve Injury spares a proportion of DRG neurons, whose axons lie in the partially degenerating Nerve, and are exposed to elevated NGF levels from Schwann and other endoneurial cells involved in Wallerian degeneration. To test the hypothesis that SP is elevated in spared DRG neurons following partial Nerve Injury, we compared the effects of complete Sciatic Nerve transection (CSNT) with those of two types of partial Injury, partial Sciatic Nerve transection (PSNT) and chronic constriction Injury (CCI). As expected, a CSNT profoundly decreased SP expression at 4 and 14 days postInjury, but after PSNT and CCI the levels of preprotachykinin (PPT) mRNA, assessed by in situ hybridization, and the SP immunoreactivity (SP-IR) of the L4 and L5 DRGs did not decrease, nor did dorsal horn SP-IR decrease. Using retrograde labelling with fluorogold to identify spared DRG neurons, we found that the proportion of these neurons expressing SP-IR 14 days after Injury was much higher than in neurons of normal DRGs. Further, the highest levels of SP-IR in individual neurons were detected in ipsilateral L4 and L5 DRG neurons after PSNT and CCI. We conclude that partial Sciatic Nerve Injury elevates SP levels in spared DRG neurons. This phenomenon might be involved in the development of neuropathic pain, which commonly follows partial Nerve Injury.

Michal Dadon-nachum - One of the best experts on this subject based on the ideXlab platform.

  • Stem cells treatment for Sciatic Nerve Injury
    Expert opinion on biological therapy, 2011
    Co-Authors: Michal Dadon-nachum, Eldad Melamed, Daniel Offen
    Abstract:

    Introduction: Sciatic Nerve Injury is common and usually results in degeneration of the distal axons and muscle denervation. Chronic muscle atrophy and fibrosis limit the recovery of muscle functio...

  • Differentiated Mesenchymal Stem Cells for Sciatic Nerve Injury
    Stem cell reviews and reports, 2011
    Co-Authors: Michal Dadon-nachum, Eldad Melamed, Ofer Sadan, I. Srugo, Daniel Offen
    Abstract:

    Sciatic Nerve Injury is common and may cause neurological deficits. Previous studies showed that administration of neurotrophic factors (NTFs), naturally occurring proteins that support the development and survival of neurons, preserved and protected damaged motor neuron in the injured Sciatic Nerve. We have been successful in converting bone marrow-derived mesenchymal stem cells into astrocyte-like cells that produce and secrete NTFs (NTF(+) cells). These cells demonstrate typical astrocyte morphology, express characteristic astrocyte markers and secrete high levels of NTFs. We have already shown that these cells and their conditioned media can protect neurons in culture and in animal models of neurodegenerative diseases. In the current study we examined whether NTF(+) cells are capable of rescuing motor neurons in a rat Sciatic Nerve Injury model, where the right hind limb Sciatic Nerve was crushed. Rats were transplanted with NTF(+) cells, MSCs or PBS into the lesion site. In rats injected with the NTF(+) cells motor function was markedly preserved. Moreover, NTF(+) cells significantly inhibited the degeneration of the neuromuscular junctions and preserved the myelinated motor axons. Our findings suggest that autologous therapeutic approach can alleviate signs of Sciatic Nerve Injury and probably other neurological disorders.