The Experts below are selected from a list of 8964 Experts worldwide ranked by ideXlab platform
Daniel T Chiu - One of the best experts on this subject based on the ideXlab platform.
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high throughput counting and superresolution mapping of tetraspanins on exosomes using a single molecule sensitive flow technique and transistor like Semiconducting Polymer dots
Angewandte Chemie, 2021Co-Authors: Yifei Jiang, Haobin Chen, Bryant S Fujimoto, Luca A Andronico, Seungryoung Jung, Lucia Vojtech, Daniel T ChiuAbstract:Here, we describes a method for high-throughput counting and superresolution mapping of surface proteins on exosomes, using a combination of a single-molecule sensitive flow technique and an adaptive superresolution imaging method. Exosomes stained with membrane dye and dye-conjugated antibodies were analyzed using a microfluidic platform at a flow rate of 100 exosome/s to determine size and protein copy number. Superresolution mapping was performed with exosomes labeled with novel transistor-like, Semiconducting Polymer dots (Pdots), which exhibit spontaneous blinking with <5 nm localization error and a broad range of optical-adjustable duty cycles. Based on the copy numbers extracted from the flow analysis, the switch-on frequency of the Pdots were fine adjusted so that structures of hundreds of exosomes can be obtained within five minutes. The high throughput and high sensitivity of this method offer clear advantages for characterization of exosomes and similar biological vesicles.
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dual mode superresolution imaging using charge transfer dynamics in Semiconducting Polymer dots
Angewandte Chemie, 2020Co-Authors: Yifei Jiang, Haobin Chen, Daniel T Chiu, Jicheng Zhang, Jason McneillAbstract:In a conjugated Polymer-based single-particle heterojunction, stochastic fluctuations of the photogenerated hole population lead to spontaneous fluorescence switching. We found that 405 nm irradiation can induce charge recombination and activate the single-particle emission. Based on these phenomena, we developed a novel class of Semiconducting Polymer dots that can operate in two superresolution imaging modes. The spontaneous switching mode offers efficient imaging of large areas, with <10 nm localization precision, while the photoactivation/deactivation mode offers slower imaging, with further improved localization precision (ca. 1 nm), showing advantages in resolving small structures that require high spatial resolution. Superresolution imaging of microtubules and clathrin-coated pits was demonstrated, under both modes. The excellent localization precision and versatile imaging options provided by these nanoparticles offer clear advantages for imaging of various biological systems.
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purification of Semiconducting Polymer dots by size exclusion chromatography prior to cytotoxicity assay and stem cell labeling
Analytical Chemistry, 2018Co-Authors: Dandan Chen, Ye Yuan, Daniel T ChiuAbstract:Semiconducting Polymer dots (Pdots) as fluorescent probes have shown promising applications because of their excellent optical properties. However, apparent differences were observed in cytotoxicity assays, which might originate from impurities introduced in Polymer synthesis or nanoparticle preparation. A simple gel-filtration-based purification method was used to address this issue. Purified Pdots displayed obviously decreased cytotoxicity as compared with the same batch of unpurified Pdots. The purified Pdots were further examined in a cytotoxicity study on mesenchymal stem cells (MSCs), which are very sensitive to exogenous probes. The results indicated that purified Pdots did not affect the proliferation ability of MSCs, while unpurified Pdots could have obvious cytotoxicity. In addition, the purified Pdots did not show cytotoxicity even after 6 months of storage. Our results demonstrated that gel filtration is an effective method for obtaining Pdots with minimal cytotoxicity, which are more suitable...
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Improving the Photostability of Semiconducting Polymer Dots Using Buffers
2018Co-Authors: Chunting Kuo, Lei Chen, Daniel T ChiuAbstract:The photostability of fluorescent probes is critical in biological imaging, especially for long-term observational analyses. Here, we describe a simple and universal method to improve the photostability of Semiconducting Polymer dots (Pdots) and other fluorescent probes by using buffers. Using Pdots as a model system, we found that HEPES or MES buffer can improve the photostability of Pdots by a factor of 20. Through a systematic study, we show that Pdot photobleaching is dominated by photoinduced radicals which can be quenched by the piperazine or morpholine structures of these buffers, which act as radical scavengers. For conditions where choice of buffer is limited, we designed fluorescent Polymers conjugated with radical scavengers to improve Pdot photostability. We then demonstrate a practical application in which HEPES buffer is used to improve the photostability of Pdots during cell imaging
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lanthanide coordinated Semiconducting Polymer dots used for flow cytometry and mass cytometry
Angewandte Chemie, 2017Co-Authors: Chunting Kuo, Quinn Degottardi, William W Kwok, Daniel T ChiuAbstract:Simultaneous monitoring of biomarkers as well as single-cell analyses based on flow cytometry and mass cytometry are important for investigations of disease mechanisms, drug discovery, and signaling-network studies. Flow cytometry and mass cytometry are complementary to each other; however, probes that can satisfy all the requirements for these two advanced technologies are limited. In this study, we report a probe of lanthanide-coordinated Semiconducting Polymer dots (Pdots), which possess fluorescence and mass signals. We demonstrated the usage of this dual-functionality probe for both flow cytometry and mass cytometry in a mimetic cell mixture and human peripheral blood mononuclear cells as model systems. The probes not only offer high fluorescence signal for use in flow cytometry, but also show better performance in mass cytometry than the commercially available counterparts.
Xu Zhen - One of the best experts on this subject based on the ideXlab platform.
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a Semiconducting Polymer nano prodrug for hypoxia activated photodynamic cancer therapy
Angewandte Chemie, 2019Co-Authors: Dong Cui, Xu Zhen, Jiaguo Huang, Yuyan JiangAbstract:Photodynamic therapy (PDT) holds great promise for cancer therapy; however, its efficacy is often compromised by tumor hypoxia. Herein, we report the synthesis of a Semiconducting Polymer nanoprodrug (SPNpd) that not only efficiently generates singlet oxygen (1 O2 ) under NIR photoirradiation but also specifically activates its chemotherapeutic action in hypoxic tumor microenvironment. SPNpd is self-assembled from a amphiphilic Polymer brush, which comprises a light-responsive photodynamic backbone grafted with poly(ethylene glycol) and conjugated with a chemodrug through hypoxia-cleavable linkers. The well-defined and compact nanostructure of SPNpd (30 nm) enables accumulation in the tumor of living mice. Owing to these features, SPNpd exerts synergistic photodynamic and chemo-therapy, and effectively inhibits tumor growth in a xenograft tumor mouse model. This study represents the first hypoxia-activatable phototherapeutic Polymeric prodrug system with a high potential for cancer therapy.
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metabolizable Semiconducting Polymer nanoparticles for second near infrared photoacoustic imaging
Advanced Materials, 2019Co-Authors: Yuyan Jiang, Xu Zhen, Chen Xie, Jiaguo Huang, Paul Kumar Upputuri, Ziling Zeng, Arunima Sharma, Manojit PramanikAbstract:Photoacoustic (PA) imaging in the second near-infrared (NIR-II) window (1000-1700 nm) holds great promise for deep-tissue diagnosis due to the reduced light scattering and minimized tissue absorption; however, exploration of such a noninvasive imaging technique is greatly constrained by the lack of biodegradable NIR-II absorbing agents. Herein, the first series of metabolizable NIR-II PA agents are reported based on Semiconducting Polymer nanoparticles (SPNs). Such completely organic nanoagents consist of π-conjugated yet oxidizable optical Polymer as PA generator and hydrolyzable amphiphilic Polymer as particle matrix to provide water solubility. The obtained SPNs are readily degraded by myeloperoxidase and lipase abundant in phagocytes, transforming from nonfluorescent nanoparticles (30 nm) into NIR fluorescent ultrasmall metabolites (≈1 nm). As such, these nanoagents can be effectively cleared out via both hepatobiliary and renal excretions after systematic administration, leaving no toxicity to living mice. Particularly these nanoagents possess high photothermal conversion efficiencies and emit bright PA signals at 1064 nm, enabling sensitive NIR-II PA imaging of both subcutaneous tumor and deep brain vasculature through intact skull in living animals at a low systematic dosage. This study thus provides a generalized molecular design toward organic metabolizable Semiconducting materials for biophotonic applications in NIR-II window.
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Metabolizable Semiconducting Polymer nanoparticles for second near-infrared photoacoustic imaging
'Wiley', 2019Co-Authors: Jiang Yuyan, Xu Zhen, Upputuri, Paul Kumar, Xie Chen, Zeng Ziling, Sharma Arunima, Li Jingchao, Huang Jiaguo, Pramanik Manojit, Pu KanyiAbstract:Photoacoustic (PA) imaging in the second near‐infrared (NIR‐II) window (1000–1700 nm) holds great promise for deep‐tissue diagnosis due to the reduced light scattering and minimized tissue absorption; however, exploration of such a noninvasive imaging technique is greatly constrained by the lack of biodegradable NIR‐II absorbing agents. Herein, the first series of metabolizable NIR‐II PA agents are reported based on Semiconducting Polymer nanoparticles (SPNs). Such completely organic nanoagents consist of π‐conjugated yet oxidizable optical Polymer as PA generator and hydrolyzable amphiphilic Polymer as particle matrix to provide water solubility. The obtained SPNs are readily degraded by myeloperoxidase and lipase abundant in phagocytes, transforming from nonfluorescent nanoparticles (30 nm) into NIR fluorescent ultrasmall metabolites (≈1 nm). As such, these nanoagents can be effectively cleared out via both hepatobiliary and renal excretions after systematic administration, leaving no toxicity to living mice. Particularly these nanoagents possess high photothermal conversion efficiencies and emit bright PA signals at 1064 nm, enabling sensitive NIR‐II PA imaging of both subcutaneous tumor and deep brain vasculature through intact skull in living animals at a low systematic dosage. This study thus provides a generalized molecular design toward organic metabolizable Semiconducting materials for biophotonic applications in NIR‐II window.MOE (Min. of Education, S’pore)Accepted versio
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cell membrane coated Semiconducting Polymer nanoparticles for enhanced multimodal cancer phototheranostics
ACS Nano, 2018Co-Authors: Xu Zhen, Yuyan Jiang, Yan Lyu, Jiaguo HuangAbstract:Phototheranostic nanoagents are promising for early diagnosis and precision therapy of cancer. However, their imaging ability and therapeutic efficacy are often limited due to the presence of delivery barriers in the tumor microenvironment. Herein, we report the development of organic multimodal phototheranostic nanoagents that can biomimetically target cancer-associated fibroblasts in the tumor microenvironment for enhanced multimodal imaging-guided cancer therapy. Such biomimetic nanocamouflages comprise a near-infrared (NIR) absorbing Semiconducting Polymer nanoparticle (SPN) coated with the cell membranes of activated fibroblasts. The homologous targeting mechanism allows the activated fibroblast cell membrane coated SPN (AF-SPN) to specifically target cancer-associated fibroblasts, leading to enhanced tumor accumulation relative to the uncoated and cancer cell membrane coated counterparts after systemic administration in living mice. As such, AF-SPN not only provides stronger NIR fluorescence and photoacoustic signals to detect tumors but also generates enhanced cytotoxic heat and singlet oxygen to exert combinational photothermal and photodynamic therapy, ultimately leading to an antitumor efficacy higher than that of the counterparts. This study introduces an organic phototheranostic system that biomimetically targets the component in the tumor microenvironment for enhanced multimodal cancer theranostics.
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temperature correlated afterglow of a Semiconducting Polymer nanococktail for imaging guided photothermal therapy
Angewandte Chemie, 2018Co-Authors: Xu Zhen, Chen XieAbstract:Nanoparticles for photothermal therapy: Real-time temperature monitoring is critical to reduce the nonspecific damage during photothermal therapy (PTT); however, PTT agents that can emit temperature-related signals are rare and limited to few inorganic nanoparticles. We herein synthesize a Semiconducting Polymer nanococktail (SPNCT ) that can not only convert photo-energy to heat but also emit temperature-correlated luminescence after cessation of light excitation. Such an afterglow luminescence of the SPNCT detects tumors more sensitively than fluorescence as a result of the elimination of tissue autofluorescence, while its temperature-dependent nature allows tumor temperature to be optically monitored under near-infrared (NIR) laser irradiation. Thus, SPNCT represents the first organic optical nanosystem that enables optical-imaging guided PTT without real-time light excitation.
Yang-hsiang Chan - One of the best experts on this subject based on the ideXlab platform.
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polymethine based Semiconducting Polymer dots with narrow band emission and absorption emission maxima at nir ii for bioimaging
Angewandte Chemie, 2020Co-Authors: Ming-ho Liu, Zhe Zhang, Yu-chi Yang, Yang-hsiang ChanAbstract:Deep-penetration fluorescence imaging in the second near-infrared (NIR-II) window heralds a new era of clinical surgery, in which high-resolution vascular/lymphatic anatomy and detailed cancerous tissues can be visualized in real time. Although several types of fluorescent agents, including inorganic nanostructures and small organic dyes have been extensively developed recently, only a few successful examples of NIR-II fluorescing Polymers with much enhanced fluorescence brightness and photo/colloidal stability have been reported. Here we describe a series of polymethine-based Semiconducting Polymers with intrinsic emission maxima in the NIR-IIa (1300-1400 nm) window and absorption maxima ranging from 1082 to 1290 nm. We prepared these Polymers as Semiconducting Polymer dots (Pdots) in aqueous solutions with fluorescence quantum yields of 0.05-0.18 %, and demonstrated their promising applications in noninvasive through-skull brain imaging in live mice with remarkable spatial resolution as well as signal-to-background contrast. This study offers a universal platform for future designing NIR-IIa or even NIR-IIb emitting Pdots.
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molecular engineering and design of Semiconducting Polymer dots with narrow band near infrared emission for in vivo biological imaging
ACS Nano, 2017Co-Authors: Chiachia Fang, Xuanjun Zhang, Po-jung Tseng, Jiaying Yan, Joseph R Pyle, Chuanpin Chen, Shuyi Lin, Jixin Chen, Yang-hsiang ChanAbstract:This article describes the design and synthesis of donor–bridge–acceptor-based Semiconducting Polymer dots (Pdots) that exhibit narrow-band emissions, ultrahigh brightness, and large Stokes shifts in the near-infrared (NIR) region. We systematically investigated the effect of π-bridges on the fluorescence quantum yields of the donor–bridge–acceptor-based Pdots. The Pdots could be excited by a 488 or 532 nm laser and have a high fluorescence quantum yield of 33% with a Stokes shift of more than 200 nm. The emission full width at half-maximum of the Pdots can be as narrow as 29 nm, about 2.5 times narrower than that of inorganic quantum dots at the same emission wavelength region. The average per-particle brightness of the Pdots is at least 3 times larger than that of the commercially available quantum dots. The excellent biocompatibility of these Pdots was demonstrated in vivo, and their specific cellular labeling capability was also approved by different cell lines. By taking advantage of the durable brig...
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Dual Colorimetric and Fluorescent Authentication Based on Semiconducting Polymer Dots for Anticounterfeiting Applications
2017Co-Authors: Wei-kai Tsai, Yung-sheng Lai, Po-jung Tseng, Chia-hsien Liao, Yang-hsiang ChanAbstract:Semiconducting Polymer dots (Pdots) have recently emerged as a novel type of ultrabright fluorescent probes that can be widely used in analytical sensing and material science. Here, we developed a dual visual reagent based on Pdots for anticounterfeiting applications. We first designed and synthesized two types of photoswitchable Pdots by incorporating photochromic dyes with multicolor Semiconducting Polymers to modulate their emission intensities and wavelengths. The resulting full-color Pdot assays showed that the colorimetric and fluorescent dual-readout abilities enabled the Pdots to serve as an anticounterfeiting reagent with low background interference. We also doped these Pdots into flexible substrates and prepared these Pdots as inks for pen handwriting as well as inkjet printing. We further applied this reagent in printing paper and checks for high-security anticounterfeiting purposes. We believe that this dual-readout method based on Pdots will create a new avenue for developing new generations of anticounterfeiting technologies
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Semiconducting Polymer Nanoparticles as Fluorescent Probes for Biological Imaging and Sensing
Particle & Particle Systems Characterization, 2014Co-Authors: Yang-hsiang ChanAbstract:In recent years, Semiconducting Polymer nanoparticles have emerged as a new class of extraordinarily bright fluorescent probes. These Polymer nanoparticles, which are primarily composed of π-conjugated Polymers, exhibit a variety of outstanding features, including exceptional fluorescence brightness, fast radiative rate, good photostability, facile surface functionalization, and low cytotoxicity. These advantageous characteristics make Polymer nanoparticles highly promising for applications in biological imaging and sensing. This progress report highlights recent advances in the synthesis, characterization, and applications as bio-labels or sensors of these highly emissive organic nanoparticles.
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multicolor fluorescent Semiconducting Polymer dots with narrow emissions and high brightness
ACS Nano, 2013Co-Authors: Yu Rong, Yang-hsiang Chan, Maria Elena Gallina, Xuanjun Zhang, Kajsa Uvdal, Maxwell Zeigler, Yong Zhang, Wei Sun, Daniel T ChiuAbstract:Fluorescent Semiconducting Polymer dots (Pdots) have attracted great interest because of their superior characteristics as fluorescent probes, such as high fluorescence brightness, fast radiative rates, and excellent photostability. However, currently available Pdots generally exhibit broad emission spectra, which significantly limit their usefulness in many biological applications involving multiplex detections. Here, we describe the design and development of multicolor narrow emissive Pdots based on different boron dipyrromethene (BODIPY) units. BODIPY-containing Semiconducting Polymers emitting at multiple wavelengths were synthesized and used as precursors for preparing the Pdots, where intraparticle energy transfer led to highly bright, narrow emissions. The emission full width at half-maximum of the resulting Pdots varies from 40 to 55 nm, which is 1.5–2 times narrower than those of conventional Semiconducting Polymer dots. BODIPY 520 Pdots were about an order of magnitude brighter than commercial Q...
Jianguo Mei - One of the best experts on this subject based on the ideXlab platform.
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Semiconducting Polymer blends that exhibit stable charge transport at high temperatures
Science, 2018Co-Authors: Aristide Gumyusenge, Yan Zhao, Alexander L Ayzner, Dung T Tran, Xuyi Luo, Gregory M Pitch, Kaelon A Jenkins, Tim J Dunn, Brett M Savoie, Jianguo MeiAbstract:Although high-temperature operation (i.e., beyond 150°C) is of great interest for many electronics applications, achieving stable carrier mobilities for organic semiconductors at elevated temperatures is fundamentally challenging. We report a general strategy to make thermally stable high-temperature Semiconducting Polymer blends, composed of interpenetrating semicrystalline conjugated Polymers and high glass-transition temperature insulating matrices. When properly engineered, such Polymer blends display a temperature-insensitive charge transport behavior with hole mobility exceeding 2.0 cm2/V·s across a wide temperature range from room temperature up to 220°C in thin-film transistors.
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Semiconducting Polymer nanoparticles for centimeters deep photoacoustic imaging in the second near infrared window
Advanced Materials, 2017Co-Authors: Liya You, Saadia T Chaudhry, Jixi Cheng, Hyeon-jeong Lee, Jianguo MeiAbstract:Thienoisoindigo-based Semiconducting Polymer with a strong near-infrared absorbance is synthesized and its water-dispersed nanoparticles (TSPNs) are investigated as a contrast agent for photoacoustic (PA) imaging in the second near-infrared (NIR-II) window (1000–1350 nm). The TSPNs generate a strong PA signal in the NIR-II optical window, where background signals from endogenous contrast agents, including blood and lipid, are at the local minima. By embedding a TSPN-containing tube in chicken-breast tissue, an imaging depth of more than 5 cm at 1064 nm excitation is achieved with a contrast-agent concentration as low as 40 µg mL−1. The TSPNs under the skin or in the tumor are clearly visualized at 1100 and 1300 nm, with negligible interference from the tissue background. TSPN as a PA contrast in the NIR-II window opens new opportunities for biomedical imaging of deep tissues with improved contrast.
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diketopyrrolopyrrole based Semiconducting Polymer nanoparticles for in vivo photoacoustic imaging
Advanced Materials, 2015Co-Authors: Jianguo Mei, Jesse V Jokerst, Adam J Shuhendler, Guosong Hong, Alexander L Antaris, Niladri Chattopadhyay, Tadanori Kurosawa, Yan Zhou, Sanjiv S GambhirAbstract:Diketopyrrolopyrrole-based Semiconducting Polymer nanoparticles with high photostability and strong photoacoustic brightness are designed and synthesized, which results in 5.3-fold photoacoustic signal enhancement in tumor xenografts after systemic administration.
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Semiconducting Polymer nanoparticles as photoacoustic molecular imaging probes in living mice
Nature Nanotechnology, 2014Co-Authors: Adam J Shuhendler, Jianguo Mei, Jesse V Jokerst, Sanjiv S Gambhir, Zhenan Bao, Jianghong RaoAbstract:Photoacoustic imaging holds great promise for the visualization of physiology and pathology at the molecular level with deep tissue penetration and fine spatial resolution. To fully utilize this potential, photoacoustic molecular imaging probes have to be developed. Here, we introduce near-infrared light absorbing Semiconducting Polymer nanoparticles as a new class of contrast agents for photoacoustic molecular imaging. These nanoparticles can produce a stronger signal than the commonly used single-walled carbon nanotubes and gold nanorods on a per mass basis, permitting whole-body lymph-node photoacoustic mapping in living mice at a low systemic injection mass. Furthermore, the Semiconducting Polymer nanoparticles possess high structural flexibility, narrow photoacoustic spectral profiles and strong resistance to photodegradation and oxidation, enabling the development of the first near-infrared ratiometric photoacoustic probe for in vivo real-time imaging of reactive oxygen species--vital chemical mediators of many diseases. These results demonstrate Semiconducting Polymer nanoparticles to be an ideal nanoplatform for developing photoacoustic molecular probes.
Yuyan Jiang - One of the best experts on this subject based on the ideXlab platform.
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transformable hybrid Semiconducting Polymer nanozyme for second near infrared photothermal ferrotherapy
Nature Communications, 2020Co-Authors: Yuyan Jiang, Hongwei Duan, Yansong Miao, Jiaguo Huang, He Sun, Xuhui Zhao, Paul Kumar Upputuri, Xiao Han, Manojit Pramanik, Ruiping ZhangAbstract:Despite its growing promise in cancer treatment, ferrotherapy has low therapeutic efficacy due to compromised Fenton catalytic efficiency in tumor milieu. We herein report a hybrid Semiconducting nanozyme (HSN) with high photothermal conversion efficiency for photoacoustic (PA) imaging-guided second near-infrared photothermal ferrotherapy. HSN comprises an amphiphilic Semiconducting Polymer as photothermal converter, PA emitter and iron-chelating Fenton catalyst. Upon photoirradiation, HSN generates heat not only to induce cytotoxicity but also to enhance Fenton reaction. The increased ·OH generation promotes both ferroptosis and apoptosis, oxidizes HSN (42 nm) and transforms it into tiny segments (1.7 nm) with elevated intratumoral permeability. The non-invasive seamless synergism leads to amplified therapeutic effects including a deep ablation depth (9 mm), reduced expression of metastasis-related proteins and inhibition of metastasis from primary tumor to distant organs. Thereby, our study provides a generalized nanozyme strategy to compensate both ferrotherapy and phototherapeutics for complete tumor regression.
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near infrared photoactivatable Semiconducting Polymer nanoblockaders for metastasis inhibited combination cancer therapy
Advanced Materials, 2019Co-Authors: Dong Cui, Yuyan Jiang, Jiaguo Huang, Penghui ChengAbstract:Inhibition of protein biosynthesis is a promising strategy to develop new therapeutic modalities for cancers; however, noninvasive precise regulation of this cellular event in living systems has been rarely reported. In this study, a Semiconducting Polymer nanoblockader (SPNB ) is developed that can inhibit intracellular protein synthesis upon near-infrared (NIR) photoactivation to synergize with photodynamic therapy (PDT) for metastasis-inhibited cancer therapy. SPNB is self-assembled from an amphiphilic Semiconducting Polymer which is grafted with poly(ethylene glycol) conjugated with a protein biosynthesis blockader through a singlet oxygen (1 O2 ) cleavable linker. Such a designed molecular structure not only enables generation of 1 O2 under NIR photoirradiation for PDT, but also permits photoactivation of blockaders to terminate protein translation. Thereby, SPNB exerts a synergistic action to afford an enhanced therapeutic efficacy in tumor ablation. More importantly, SPNB -mediated photoactivation of protein synthesis inhibition precisely and remotely downregulates the expression levels of metastasis-related proteins in tumor tissues, eventually contributing to the complete inhibition of lung metastasis. This study thus proposes a photoactivatable protherapeutic design for metastasis-inhibited cancer therapy.
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a Semiconducting Polymer nano prodrug for hypoxia activated photodynamic cancer therapy
Angewandte Chemie, 2019Co-Authors: Dong Cui, Xu Zhen, Jiaguo Huang, Yuyan JiangAbstract:Photodynamic therapy (PDT) holds great promise for cancer therapy; however, its efficacy is often compromised by tumor hypoxia. Herein, we report the synthesis of a Semiconducting Polymer nanoprodrug (SPNpd) that not only efficiently generates singlet oxygen (1 O2 ) under NIR photoirradiation but also specifically activates its chemotherapeutic action in hypoxic tumor microenvironment. SPNpd is self-assembled from a amphiphilic Polymer brush, which comprises a light-responsive photodynamic backbone grafted with poly(ethylene glycol) and conjugated with a chemodrug through hypoxia-cleavable linkers. The well-defined and compact nanostructure of SPNpd (30 nm) enables accumulation in the tumor of living mice. Owing to these features, SPNpd exerts synergistic photodynamic and chemo-therapy, and effectively inhibits tumor growth in a xenograft tumor mouse model. This study represents the first hypoxia-activatable phototherapeutic Polymeric prodrug system with a high potential for cancer therapy.
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metabolizable Semiconducting Polymer nanoparticles for second near infrared photoacoustic imaging
Advanced Materials, 2019Co-Authors: Yuyan Jiang, Xu Zhen, Chen Xie, Jiaguo Huang, Paul Kumar Upputuri, Ziling Zeng, Arunima Sharma, Manojit PramanikAbstract:Photoacoustic (PA) imaging in the second near-infrared (NIR-II) window (1000-1700 nm) holds great promise for deep-tissue diagnosis due to the reduced light scattering and minimized tissue absorption; however, exploration of such a noninvasive imaging technique is greatly constrained by the lack of biodegradable NIR-II absorbing agents. Herein, the first series of metabolizable NIR-II PA agents are reported based on Semiconducting Polymer nanoparticles (SPNs). Such completely organic nanoagents consist of π-conjugated yet oxidizable optical Polymer as PA generator and hydrolyzable amphiphilic Polymer as particle matrix to provide water solubility. The obtained SPNs are readily degraded by myeloperoxidase and lipase abundant in phagocytes, transforming from nonfluorescent nanoparticles (30 nm) into NIR fluorescent ultrasmall metabolites (≈1 nm). As such, these nanoagents can be effectively cleared out via both hepatobiliary and renal excretions after systematic administration, leaving no toxicity to living mice. Particularly these nanoagents possess high photothermal conversion efficiencies and emit bright PA signals at 1064 nm, enabling sensitive NIR-II PA imaging of both subcutaneous tumor and deep brain vasculature through intact skull in living animals at a low systematic dosage. This study thus provides a generalized molecular design toward organic metabolizable Semiconducting materials for biophotonic applications in NIR-II window.
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Multimodal Biophotonics of Semiconducting Polymer Nanoparticles.
Accounts of chemical research, 2018Co-Authors: Yuyan JiangAbstract:ConspectusBiophotonics as an interdisciplinary frontier plays an increasingly important role in modern biomedical science. Optical agents are generally involved in biophotonics to interpret biomolecular events into readable optical signals for imaging and diagnosis or to convert photons into other forms of energy (such as heat, mechanical force, or chemical radicals) for therapeutic intervention and biological stimulation. Development of new optical agents including metallic nanoparticles, quantum dots, up-conversion nanoparticles, carbon dots, and silica nanoparticles has contributed to the advancement of this field. However, most of these agents have their own merits and demerits, making them less effective as multimodal biophotonic platforms.In this Account, we summarize our recent work on the development of near-infrared (NIR) Semiconducting Polymer nanoparticles (SPNs) as multimodal light converters for advanced biophotonics. SPNs are composed of π-electron delocalized Semiconducting Polymers (SPs) a...
