The Experts below are selected from a list of 9621 Experts worldwide ranked by ideXlab platform
Camilo Dias Seabra Pereira - One of the best experts on this subject based on the ideXlab platform.
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lead toxicity on a Sentinel Species subpopulation inhabiting mangroves with different status conservation
Chemosphere, 2020Co-Authors: Luis Duarte, Julian Blasco, M G M Catharino, Edson G Moreira, Chiara Trombini, Caio Rodrigues Nobre, Beatriz Barbosa Moreno, Denis Moledo De Souza Abessa, Camilo Dias Seabra PereiraAbstract:Abstract Lead is a priority pollutant introduced in the aquatic environment by different sources commonly located in estuarine regions, such as ports, marinas and industries. Environmental agencies around the world set the maximum allowable concentration of lead in effluents, surface water and sediment, but few studies reported its accumulation and chronic toxicity in mangrove benthic invertebrates using concentrations believed to be safe. In the case of Brazilian mangrove environments, Ucides cordatus is a crab Species of choice to be used in bioaccumulation studies. We have assessed biomarkers’ responses (DNA strand breaks, micronucleated cells, metallothioneins, enzymatic activity of aminolevulinic acid dehydratase and neutral red retention time) and the total bioaccumulation in six tissues of U. cordatus crabs resident to mangrove areas under different conservation status during a 28-day period bioassay. We also investigated Pb subcellular partition and biomarkers’ responses using a supposedly safe concentration (10 μg L−1). During the Pb exposure, the highest concentration of Pb was observed in crab gills. Crabs also showed a high ability to allocate Pb in detoxified forms. Multivariate analysis pointed out that bioaccumulation (total, active and detoxified) is linked to biomarkers. Even in supposedly safe dosage, U. cordatus triggered its defense mechanisms expressing more metallothioneins and presented relevant cyto-genotoxic damage. Our data suggest the development of biological tolerance to Pb in crabs from polluted areas. Our results provided a new insight about lead toxicity even at concentrations considered environmentally safe, which could support new strategies to manage estuarine areas considering their respective conservation status.
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mangrove metal pollution induces biological tolerance to cd on a crab Sentinel Species subpopulation
Science of The Total Environment, 2019Co-Authors: Luis Duarte, M G M Catharino, Edson G Moreira, Chiara Trombini, Julian Blasco Moreno, Camilo Dias Seabra PereiraAbstract:Abstract Metals are persistent pollutants, able to accumulate in the biota and magnify in trophic web. In the specific case of cadmium contamination, it has been the subject of considerable interest in recent years because of its biological effects and it is one of major pollutant in estuarine areas. Ucides cordatus is considered a mangrove local Sentinel crab Species in Brazil and there are previous studies reporting crab subpopulations living from pristine to heavily metal impacted areas in Sao Paulo coast (Southeastern Brazil). Taking into account the background knowledge about these subpopulations, we proposed the hypothesis that crabs from a highly polluted mangrove (Cubatao - CUB) have developed biological tolerance to cadmium compared to animals from an Environmental Protected Area (Jureia - JUR). Aiming to verify this hypothesis, we have investigated total bioaccumulation and subcellular partition of Cd, besides biomarkers' responses during a long-term exposure bioassay (28 days, with weekly sampling) using a supposedly safe Cd concentration (0.0022 mg L−1). Specimens from the pristine area (JUR) accumulated higher total Cd, as such as in its biologically active form in gills. Animals living in the polluted site (CUB) presented higher amounts of Cd in the mainly detoxifying tissue (hepatopancreas), which could be considered a pathway leading to tolerance for this metal. Multivariate analysis indicated that bioaccumulation (active, detoxified and total Cd) is linked to geno-cytotoxic damages. CUB subpopulation was considered more tolerant since it presented proportionally less damage and more capacity to allocate Cd in the main detoxifying forms and tissues.
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metal toxicity assessment by Sentinel Species of mangroves in situ case study integrating chemical and biomarkers analyses
Ecotoxicology and Environmental Safety, 2017Co-Authors: Luis Duarte, Caroline Araujo De Souza, Camilo Dias Seabra Pereira, Marcelo Antonio Amaro PinheiroAbstract:Globally, there is a lack of knowledge about tropical ecotoxicology dealing with the potential impact of metal contamination in mangrove ecosystem. This habitat is considered a nursery for several animal Species, among them the "uca"-crab (Ucides cordatus), known as a key Species due to its biological and economical importance. This study evaluated the association involving metal contamination (Cd, Cu, Pb, Cr, Mn and Hg) in water, sediment, red-mangrove vegetation (Rhizophora mangle) and tissues of uca crab, together with its geno-cytotoxic responses, based on micronucleated hemocytes frequency and the retention time of neutral red in lysosomes. We assessed six mangrove areas with distinct pollution levels in Sao Paulo State, Brazil, where the water and sediment contamination by metals were associated with accumulation of these pollutants in biotic compartments (mangrove leaves and crab). In U. cordatus, metal accumulation was best explained by metal concentration found in leaves of R. mangle than in the water or sediment, indicating that feeding drives metal exposure in this organism. Mercury (Hg) concentration in sediment, copper (Cu) concentration in hepatopancreas of U. cordatus and lead (Pb) in water and green leaves of R. mangle showed a significant correlation with genotoxic impact in U. cordatus. However, copper concentration (in green/senescent leaves and hepatopancreas) and lead (in sediment), were the major metals affecting lysosomal membrane integrity. Therefore, representatives of all compartments were associated with cyto and genotoxicity in this Species, thus requiring a holistic approach to issues related to sublethal damage. Probability estimates of cytogenetic impacts related to metal concentration in abiotic compartments (significantly correlated with known biomarkers: Hg in sediment; and Pb in water and sediment) are also presented. Our results highlight the need for environmental restoration of mangroves areas contaminated with metals, responsible for cytogenetic injuries and revealing a pre-pathological condition in this Sentinel Species, in addition to ecological disturbances.
Luis Duarte - One of the best experts on this subject based on the ideXlab platform.
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lead toxicity on a Sentinel Species subpopulation inhabiting mangroves with different status conservation
Chemosphere, 2020Co-Authors: Luis Duarte, Julian Blasco, M G M Catharino, Edson G Moreira, Chiara Trombini, Caio Rodrigues Nobre, Beatriz Barbosa Moreno, Denis Moledo De Souza Abessa, Camilo Dias Seabra PereiraAbstract:Abstract Lead is a priority pollutant introduced in the aquatic environment by different sources commonly located in estuarine regions, such as ports, marinas and industries. Environmental agencies around the world set the maximum allowable concentration of lead in effluents, surface water and sediment, but few studies reported its accumulation and chronic toxicity in mangrove benthic invertebrates using concentrations believed to be safe. In the case of Brazilian mangrove environments, Ucides cordatus is a crab Species of choice to be used in bioaccumulation studies. We have assessed biomarkers’ responses (DNA strand breaks, micronucleated cells, metallothioneins, enzymatic activity of aminolevulinic acid dehydratase and neutral red retention time) and the total bioaccumulation in six tissues of U. cordatus crabs resident to mangrove areas under different conservation status during a 28-day period bioassay. We also investigated Pb subcellular partition and biomarkers’ responses using a supposedly safe concentration (10 μg L−1). During the Pb exposure, the highest concentration of Pb was observed in crab gills. Crabs also showed a high ability to allocate Pb in detoxified forms. Multivariate analysis pointed out that bioaccumulation (total, active and detoxified) is linked to biomarkers. Even in supposedly safe dosage, U. cordatus triggered its defense mechanisms expressing more metallothioneins and presented relevant cyto-genotoxic damage. Our data suggest the development of biological tolerance to Pb in crabs from polluted areas. Our results provided a new insight about lead toxicity even at concentrations considered environmentally safe, which could support new strategies to manage estuarine areas considering their respective conservation status.
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mangrove metal pollution induces biological tolerance to cd on a crab Sentinel Species subpopulation
Science of The Total Environment, 2019Co-Authors: Luis Duarte, M G M Catharino, Edson G Moreira, Chiara Trombini, Julian Blasco Moreno, Camilo Dias Seabra PereiraAbstract:Abstract Metals are persistent pollutants, able to accumulate in the biota and magnify in trophic web. In the specific case of cadmium contamination, it has been the subject of considerable interest in recent years because of its biological effects and it is one of major pollutant in estuarine areas. Ucides cordatus is considered a mangrove local Sentinel crab Species in Brazil and there are previous studies reporting crab subpopulations living from pristine to heavily metal impacted areas in Sao Paulo coast (Southeastern Brazil). Taking into account the background knowledge about these subpopulations, we proposed the hypothesis that crabs from a highly polluted mangrove (Cubatao - CUB) have developed biological tolerance to cadmium compared to animals from an Environmental Protected Area (Jureia - JUR). Aiming to verify this hypothesis, we have investigated total bioaccumulation and subcellular partition of Cd, besides biomarkers' responses during a long-term exposure bioassay (28 days, with weekly sampling) using a supposedly safe Cd concentration (0.0022 mg L−1). Specimens from the pristine area (JUR) accumulated higher total Cd, as such as in its biologically active form in gills. Animals living in the polluted site (CUB) presented higher amounts of Cd in the mainly detoxifying tissue (hepatopancreas), which could be considered a pathway leading to tolerance for this metal. Multivariate analysis indicated that bioaccumulation (active, detoxified and total Cd) is linked to geno-cytotoxic damages. CUB subpopulation was considered more tolerant since it presented proportionally less damage and more capacity to allocate Cd in the main detoxifying forms and tissues.
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metal toxicity assessment by Sentinel Species of mangroves in situ case study integrating chemical and biomarkers analyses
Ecotoxicology and Environmental Safety, 2017Co-Authors: Luis Duarte, Caroline Araujo De Souza, Camilo Dias Seabra Pereira, Marcelo Antonio Amaro PinheiroAbstract:Globally, there is a lack of knowledge about tropical ecotoxicology dealing with the potential impact of metal contamination in mangrove ecosystem. This habitat is considered a nursery for several animal Species, among them the "uca"-crab (Ucides cordatus), known as a key Species due to its biological and economical importance. This study evaluated the association involving metal contamination (Cd, Cu, Pb, Cr, Mn and Hg) in water, sediment, red-mangrove vegetation (Rhizophora mangle) and tissues of uca crab, together with its geno-cytotoxic responses, based on micronucleated hemocytes frequency and the retention time of neutral red in lysosomes. We assessed six mangrove areas with distinct pollution levels in Sao Paulo State, Brazil, where the water and sediment contamination by metals were associated with accumulation of these pollutants in biotic compartments (mangrove leaves and crab). In U. cordatus, metal accumulation was best explained by metal concentration found in leaves of R. mangle than in the water or sediment, indicating that feeding drives metal exposure in this organism. Mercury (Hg) concentration in sediment, copper (Cu) concentration in hepatopancreas of U. cordatus and lead (Pb) in water and green leaves of R. mangle showed a significant correlation with genotoxic impact in U. cordatus. However, copper concentration (in green/senescent leaves and hepatopancreas) and lead (in sediment), were the major metals affecting lysosomal membrane integrity. Therefore, representatives of all compartments were associated with cyto and genotoxicity in this Species, thus requiring a holistic approach to issues related to sublethal damage. Probability estimates of cytogenetic impacts related to metal concentration in abiotic compartments (significantly correlated with known biomarkers: Hg in sediment; and Pb in water and sediment) are also presented. Our results highlight the need for environmental restoration of mangroves areas contaminated with metals, responsible for cytogenetic injuries and revealing a pre-pathological condition in this Sentinel Species, in addition to ecological disturbances.
Karine Le Ménach - One of the best experts on this subject based on the ideXlab platform.
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Integrated monitoring of chemicals and their effects on four Sentinel Species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring
Marine Environmental Research, 2017Co-Authors: Thierry Burgeot, Farida Akcha, Dominique Menard, Craig Robinson, Véronique Loizeau, Christophe Brach-papa, Concepción Martínez-gòmez, Jérémie Le Goff, Hélène Budzinski, Karine Le MénachAbstract:The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine Sentinel Species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four Species in 2008–2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
Sahana Kuthyar - One of the best experts on this subject based on the ideXlab platform.
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effects of anthropogenic habitat disturbance and giardia duodenalis infection on a Sentinel Species gut bacteria
Ecology and Evolution, 2021Co-Authors: Sahana Kuthyar, Martin M Kowalewski, Dawn M Roellig, Elizabeth K Mallott, Yan Zeng, Thomas R Gillespie, Katherine R AmatoAbstract:Habitat disturbance, a common consequence of anthropogenic land use practices, creates human-animal interfaces where humans, wildlife, and domestic Species can interact. These altered habitats can influence host-microbe dynamics, leading to potential downstream effects on host physiology and health. Here, we explored the effect of ecological overlap with humans and domestic Species and infection with the protozoan parasite Giardia duodenalis on the bacteria of black and gold howler monkeys (Alouatta caraya), a key Sentinel Species, in northeastern Argentina. Fecal samples were screened for Giardia duodenalis infection using a nested PCR reaction, and the gut bacterial community was characterized using 16S rRNA gene amplicon sequencing. Habitat type was correlated with variation in A. caraya gut bacterial community composition but did not affect gut bacterial diversity. Giardia presence did not have a universal effect on A. caraya gut bacteria across habitats, perhaps due to the high infection prevalence across all habitats. However, some bacterial taxa were found to vary with Giardia infection. While A. caraya's behavioral plasticity and dietary flexibility allow them to exploit a range of habitat conditions, habitats are generally becoming more anthropogenically disturbed and, thus, less hospitable. Alterations in gut bacterial community dynamics are one possible indicator of negative health outcomes for A. caraya in these environments, since changes in host-microbe relationships due to stressors from habitat disturbance may lead to negative repercussions for host health. These dynamics are likely relevant for understanding organism responses to environmental change in other mammals.
Thierry Burgeot - One of the best experts on this subject based on the ideXlab platform.
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Integrated monitoring of chemicals and their effects on four Sentinel Species, Limanda limanda, Platichthys flesus, Nucella lapillus and Mytilus sp., in Seine Bay: A key step towards applying biological effects to monitoring
Marine Environmental Research, 2017Co-Authors: Thierry Burgeot, Farida Akcha, Dominique Menard, Craig Robinson, Véronique Loizeau, Christophe Brach-papa, Concepción Martínez-gòmez, Jérémie Le Goff, Hélène Budzinski, Karine Le MénachAbstract:The International workshop on Integrated Assessment of CONtaminants impacts on the North sea (ICON) provided a framework to validate the application of chemical and biological assessment thresholds (BACs and EACs) in the Seine Bay in France. Bioassays (oyster larval anomalies, Corophium arenarium toxicity assay and DR Calux) for sediment and biomarkers: ethoxyresorufin-O-deethylase (EROD) activity, acetylcholinesterase (AChE) activity, lysosomal membrane stability (LMS), DNA strand breaks using the Comet assay, DNA adducts, micronucleus (MN), PAH metabolites, imposex, intersex and fish external pathologies were analysed in four marine Sentinel Species (Platichthys flesus, Limanda limanda, Mytilus sp. and Nucella lapilus). Polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs) and heavy metals were analysed in biota and sediment. Results for sediment and four Species in 2008–2009 made it possible to quantify the impact of contaminants using thresholds (Environmental Assessment Criteria/EAC2008: 70% and EAC2009: 60%) and effects (EAC2008: 50% and EAC2009: 40%) in the Seine estuary. The Seine estuary is ranked among Europe's most highly polluted sites.
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concentrations of pahs polycyclic aromatic hydrocarbons in various marine organisms in relation to those in sediments and to trophic level
Marine Pollution Bulletin, 1998Co-Authors: P Baumard, Thierry Burgeot, Hélène Budzinski, P Garrigues, J C Sorbe, J BellocqAbstract:Sediments and marine organisms such as mussels (Mytilus galloprovincialis), fish (Mullus barbatus, Serranus scriba), crabs (Polybius henslowi) and shrimp-like crustaceans (mysids, euphausiids) were sampled on European coasts, France, Spain) and analysed for their PAH content by gas chromatography/mass spectrometry (GC-MS). The PAH concentrations determined in the organisms were significantly negatively correlated with logKow values, but to different extents according to the Species. This was explained by the greater exposure of the organisms to the lower molecular weight compounds with differences resulting from different feeding habits, habitat, and biotransformation capacities of the organisms in relation to trophic levels. The influence of these observations on the use of different Sentinel Species in biomonitoring programmes is discussed.
