The Experts below are selected from a list of 1161 Experts worldwide ranked by ideXlab platform
Guoyu Huang - One of the best experts on this subject based on the ideXlab platform.
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SIRT4 is upregulated in breast cancer and promotes the proliferation, migration and invasion of breast cancer cells
International journal of clinical and experimental pathology, 2017Co-Authors: Guoyu Huang, Yao Lin, Guanbao ZhuAbstract:Background: Several members of the SIRT family (SIRT1-7), a highly conserved family of NAD+-dependent enzymes, play an important role in tumor formation. Recently, several studies have suggested that SIRT4 may function as both a tumor oncogene and a tumor suppressor. However, its relationship with breast cancer remains unclear. Methods: We investigated SIRT4 protein levels in breast cancer and its possible association with selected clinicopathological parameters by immunohistochemical staining of a tissue microarray that included samples from 94 breast cancer patients. We further invested the effect of SIRT4 on the proliferation, migration and invasion of breast cancer cells. Results: SIRT4 protein levels in breast were markedly higherthan their non-neoplastic tissue counterparts (P
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Decreased SIRT4 protein levels in endometrioid adenocarcinoma tissues are associated with advanced AJCC stage.
Cancer Biomarkers, 2017Co-Authors: Xiaoxi Chen, Xiangwen Lai, Qingxin Tian, Tingting Lei, Jingye Pan, Guoyu HuangAbstract:BACKGROUND Members of the SIRT family are a highly conserved family of NAD+-dependent enzymes, many of which (SIRT1-7) play an important role in tumor formation. Recently, several studies have suggested that SIRT4 not only regulates glutamine metabolism, but also serves as a tumor suppressor. There are no studies have assessed its clinical significance in endometrioid adenocarcinoma. METHODS We investigated SIRT4 protein levels in endometrioid adenocarcinoma and its possible association with selected clinico-pathological parameters by immunohistochemical staining of a tissue microarray that included 65 endometrioid adenocarcinoma patients. RESULTS SIRT4 protein levels in endometrioid adenocarcinoma were markedly lower than its non-neoplastic tissue counterpart (P< 0.001). Moreover, lower SIRT4 expression levels were observed in advanced AJCC stages of development (P= 0.002). CONCLUSIONS Our results indicated that SIRT4 may be involved in the development of endometrioid adenocarcinoma and is a promising target for both the diagnosis and potential therapy of endometrioid adenocarcinoma.
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SIRT4 is upregulated in breast cancer and promotes the proliferation migration and invasion of breast cancer cells
International Journal of Clinical and Experimental Pathology, 2017Co-Authors: Guoyu Huang, Yao Lin, Guanbao ZhuAbstract:Background: Several members of the SIRT family (SIRT1-7), a highly conserved family of NAD+-dependent enzymes, play an important role in tumor formation. Recently, several studies have suggested that SIRT4 may function as both a tumor oncogene and a tumor suppressor. However, its relationship with breast cancer remains unclear. Methods: We investigated SIRT4 protein levels in breast cancer and its possible association with selected clinicopathological parameters by immunohistochemical staining of a tissue microarray that included samples from 94 breast cancer patients. We further invested the effect of SIRT4 on the proliferation, migration and invasion of breast cancer cells. Results: SIRT4 protein levels in breast were markedly higherthan their non-neoplastic tissue counterparts (P<0.001). Additionally, SIRT4 promoted the proliferation, migration and invasion of breast cancer cells. Conclusions: Our results show that SIRT4 possess oncogenic properties at the human cancer cell level and indicate that SIRT4 may participate in the development of breast cancer.
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Sirtuin-4 (SIRT4) is downregulated and associated with some clinicopathological features in gastric adenocarcinoma
Biomedicine & Pharmacotherapy, 2015Co-Authors: Guoyu Huang, Feifei Cui, Meng Zhang, Huamei Tang, Zhihai PengAbstract:Abstract Background Several members of the SIRT family (SIRT1-7), which are a highly conserved family of NAD+-dependent enzymes, play an important role in tumor formation. Recently, several studies have suggested that SIRT4 can regulate glutamine metabolism yet have tumor suppressor function too. However, our understanding of SIRT4 expression and its association with the clinicopathological parameters remains poor. Method We evaluated SIRT4 protein expression levels in gastric adenocarcinoma and corresponding normal gastric tissue by immunohistochemical staining on a tissue microarray that included 75 gastric adenocarcinoma patients. We also determined the association between SIRT4 expression levels and selected clinicopathological parameters in gastric adenocarcinoma. Results We found that the expression level of SIRT4 in gastric adenocarcinoma was significantly lower than the corresponding normal tissue levels (P = 0.003). Besides, lower SIRT4 levels were observed in pathological grade (P = 0.002), depth of tumor invasion (P = 0.034), positive lymph node numbers (P = 0.005) and UICC stage (P = 0.002). Conclusions Our results support the notion that SIRT4 behaves as a tumor suppressor at the human tissue protein level. In addition, our data indicate that SIRT4 might be closely involved in the process of gastric adenocarcinoma development and it might potentially serve as a diagnostic biomarker and therapeutic target in gastric adenocarcinoma.
Guanbao Zhu - One of the best experts on this subject based on the ideXlab platform.
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SIRT4 is upregulated in breast cancer and promotes the proliferation, migration and invasion of breast cancer cells
International journal of clinical and experimental pathology, 2017Co-Authors: Guoyu Huang, Yao Lin, Guanbao ZhuAbstract:Background: Several members of the SIRT family (SIRT1-7), a highly conserved family of NAD+-dependent enzymes, play an important role in tumor formation. Recently, several studies have suggested that SIRT4 may function as both a tumor oncogene and a tumor suppressor. However, its relationship with breast cancer remains unclear. Methods: We investigated SIRT4 protein levels in breast cancer and its possible association with selected clinicopathological parameters by immunohistochemical staining of a tissue microarray that included samples from 94 breast cancer patients. We further invested the effect of SIRT4 on the proliferation, migration and invasion of breast cancer cells. Results: SIRT4 protein levels in breast were markedly higherthan their non-neoplastic tissue counterparts (P
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SIRT4 is upregulated in breast cancer and promotes the proliferation migration and invasion of breast cancer cells
International Journal of Clinical and Experimental Pathology, 2017Co-Authors: Guoyu Huang, Yao Lin, Guanbao ZhuAbstract:Background: Several members of the SIRT family (SIRT1-7), a highly conserved family of NAD+-dependent enzymes, play an important role in tumor formation. Recently, several studies have suggested that SIRT4 may function as both a tumor oncogene and a tumor suppressor. However, its relationship with breast cancer remains unclear. Methods: We investigated SIRT4 protein levels in breast cancer and its possible association with selected clinicopathological parameters by immunohistochemical staining of a tissue microarray that included samples from 94 breast cancer patients. We further invested the effect of SIRT4 on the proliferation, migration and invasion of breast cancer cells. Results: SIRT4 protein levels in breast were markedly higherthan their non-neoplastic tissue counterparts (P<0.001). Additionally, SIRT4 promoted the proliferation, migration and invasion of breast cancer cells. Conclusions: Our results show that SIRT4 possess oncogenic properties at the human cancer cell level and indicate that SIRT4 may participate in the development of breast cancer.
Bo Liu - One of the best experts on this subject based on the ideXlab platform.
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molecular and functional characterization of SIRT4 and sirt6 in megalobrama amblycephala under high glucose metabolism
Comparative Biochemistry and Physiology B, 2019Co-Authors: Shanting Lin, Linzhe Teng, Yan Lin, Linghong Miao, Jingyuan Hao, Xin Huang, Bo LiuAbstract:We analyzed the sequences of SIRT4 and sirt6 and their changes in expression after oral glucose administration in blunt snout bream (Megalobrama amblycephala). We cloned SIRT4 and sirt6 and found that their full-length cDNA sequences were 1530 bp and 1723 bp, respectively; their amino acid sequences were 93% and 92% identical to Danio rerio. Megalobrama amblycephala were fed a high glucose solution (3 g/kg). Normally, SIRT4 expression is higher in spleen, intestine, and gill (P < .05), and sirt6 expression is higher in intestine and gill (P < .05). After oral glucose administration, SIRT4 and sirt6 expression increased in liver and gill, and SIRT4 expression increased in intestine at 0.5 h (P < .05). In contrast, SIRT4 in kidney and sirt6 in head kidney were downregulated at 1 h (P < .05). Expression of SIRT4 was upregulated in brain, head kidney, spleen, muscle, and liver 2 h, 4 h, 4 h/24 h, 8 h, and 24 h, respectively (P < .05). Expression of SIRT4 was downregulated in kidney at 8 h-48 h (P < .05). Expression of sirt6 was upregulated in intestine, liver, muscle, kidney, and spleen at 4 h/24 h, 8 h/24 h, 12 h, 12 h, and 24 h, respectively (P < .05). We report that SIRT4 and sirt6 are highly conserved in evolution and exhibit tissue-specific expression profiles. We demonstrate that the expression of SIRT4 and sirt6 are tissue-specific, and depend upon tissue-specific responses to glucose metabolism.
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Molecular and functional characterization of SIRT4 and sirt6 in Megalobrama amblycephala under high glucose metabolism.
Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 2019Co-Authors: Shanting Lin, Linzhe Teng, Yan Lin, Linghong Miao, Jingyuan Hao, Xin Huang, Bo LiuAbstract:We analyzed the sequences of SIRT4 and sirt6 and their changes in expression after oral glucose administration in blunt snout bream (Megalobrama amblycephala). We cloned SIRT4 and sirt6 and found that their full-length cDNA sequences were 1530 bp and 1723 bp, respectively; their amino acid sequences were 93% and 92% identical to Danio rerio. Megalobrama amblycephala were fed a high glucose solution (3 g/kg). Normally, SIRT4 expression is higher in spleen, intestine, and gill (P
Marie-pierre Junier - One of the best experts on this subject based on the ideXlab platform.
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Sirtuin-2 Activity is Required for Glioma Stem Cell Proliferation Arrest but not Necrosis Induced by Resveratrol
Stem Cell Reviews and Reports, 2014Co-Authors: Salwa Sayd, Cecile Thirant, Elias A. El-habr, Nadia Tahiri-jouti, Alexandra Bogeas, Luiz Gustavo Dubois, Herve Chneiweiss, Joanna Lipecka, Marie-pierre JunierAbstract:Glioblastomas, the most common form of primary brain tumors, are the fourth cause of death by cancer in adults. Increasing evidences suggest that glioblastoma resistance to existing radio- and chemotherapies rely on glioblastoma stem cells (GSCs). GSCs are endowed with a unique combination of stem-like properties alike to normal neural stem cells (NSCs), and of tumor initiating properties. The natural polyphenol resveratrol is known to exert opposite actions on neural cells according to their normal or cancerous status. Here, we used resveratrol to explore the molecular mechanisms differing between GSCs and NSCs. We observed a dual action of resveratrol on GSCs: resveratrol blocked GSC proliferation up to 150 mu M and induced their necrosis at higher doses. On the opposite, resveratrol had no effect on NSC behavior. To determine the mechanisms underlying resveratrol effects, we focused our attention on the family of NAD-dependent deacetylases sirtuins (SIRT). A member of this family, SIRT1, has been repetitively shown to constitute a preferential resveratrol target, at least in normal cells. Western blot analysis showed that SIRT1 and SIRT3 were expressed by both GSCs and NSCs whereas SIRT2 expression was restricted to GSCs. Pharmacological blockade of SIRT2 activity or down-regulation of SIRT2 expression with siRNAs counteracted the inhibitory effect of resveratrol on cell proliferation. On the contrary, inhibition of SIRT2 activity or expression did not counteract GSC necrosis observed in presence of high doses of resveratrol. Our results highlight SIRT2 as a novel target for altering GSC properties.
E Verdin - One of the best experts on this subject based on the ideXlab platform.
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Sirtuins: critical regulators at the crossroads between cancer and aging
Oncogene, 2007Co-Authors: L R Saunders, E VerdinAbstract:Sirtuins (SIRTs 1−7), or class III histone deacetylases (HDACs), are protein deacetylases/ADP ribosyltransferases that target a wide range of cellular proteins in the nucleus, cytoplasm, and mitochondria for post-translational modification by acetylation (SIRT1, -2, -3 and -5) or ADP ribosylation (SIRT4 and -6). The orthologs of sirtuins in lower organisms play a critical role in regulating lifespan. As cancer is a disease of aging, we discuss the growing implications of the sirtuins in protecting against cancer development. Sirtuins regulate the cellular responses to stress and ensure that damaged DNA is not propagated and that mutations do not accumulate. SIRT1 also promotes replicative senescence under conditions of chronic stress. By participating in the stress response to genomic insults, sirtuins are thought to protect against cancer, but they are also emerging as direct participants in the growth of some cancers. Here, we review the growing implications of sirtuins both in cancer prevention and as specific and novel cancer therapeutic targets.